Highly sensitive UV-visible detectionat a wide range of needs
Agilent 1100 Series UV-visible Detectors
A choice of three detectors with high sensitivity ...
Whether you want a standalone UV-visible detector
that extends your analytical range or an automated
HPLC system with UV-visible detection for maximum
productivity, Agilent’s 1100 Series high performance
liquid chromatography (HPLC) system offers the
UV-detector that best suits your needs.
The Agilent 1100 Seriesvariable wavelengthdetector
lowers your detectionlimits with a simplifieddesign optimized forhigh sensitivity and lowbaseline drift.
The Agilent 1100 Seriesmultiwavelengthdetector
lets you detect up tofive different wave-lengths simultaneouslywithout sacrificingsensitivity.
The Agilent 1100 Seriesdiode array detector
gives you the full powerof multiwavelengthdetection and spectralinformation with thehighest resolution andsensitivity.
... that increase laboratory productivity
Configure your own Agilent 1100 Series
HPLC system from individual modules that
fit together to form an integrated system.
All detectors are designed to achieve high
sensitivity and outstanding productivity:
State-of-the-art optics.
These optics give high signal level, lowbaseline noise, low drift and minimumrefractive-index effects. A range of flowcells allows you to adapt to changingapplication needs such as columndiameter.
Convenient instrument control.
The Agilent 1100 Series handheld controlmodule offers intuitive, single-point instru-ment control and signal display in the palmof your hand. Or use the multitechniqueAgilent ChemStation for instrument controland convenient data evaluation, storageand retrieval.
Built-in GLP/GMP compliance.
Such features as automated wavelengthverification and built-in tracking of systemperformance and maintenance automati-cally document GLP compliance.
Early maintenance feedback (EMF).
Timely maintenance warnings at your ownpredefined limits help you keep yoursystem operating at maximum productivityand lowest cost.
Simple and fast maintenance.
Front access to higher-maintenance partssuch as lamps and flow cell cartridgesminimizes instrument downtime. You canadapt your methods and instrumentationin seconds.
The Agilent 1100 Series VWD provides high sensitivity and
improves productivity in routine work. This detector offers:
• Minimum baseline noise for lowest detection limits.
• Highest baseline stability and a wide linear range for reliable
sensitivity.
• Programmable wavelength switching for sensitivity and
selectivity optimized to each analyte’s elution.
• Stop-flow wavelength scanning for quick wavelength
optimization.
You achieve high sensitivity and a stable baseline.
Reengineered optics give high signal level, lowbaseline noise, low drift and minimum refractiveindex effects.
Deuterium lamp
Lens
Cut-off filterHolmiumoxide filter
Slit
Grating
Flow cell
Sample diode
Reference diode
Beamsplitter
10 pg AnthraceneBaseline noise < 1*10-5 AU
0 1 2 3 4 5Time [min]
0.30
0.25
0.20
0.15
0.10
0.05
0
mAU
Mirror 1
Mirror 2
Agilent 1100 Series variable wavelength detector –for high sensitivity and stability
You can verify wavelengthaccuracy for GLP compliance
– automatically.
The Holmium oxide filter swingsinto the light path to verify that
your wavelength is indeed whatthe setpoint says.
This is the first dedicated multiwavelength detector that offers the same high
sensitivity for both single- and multiwavelength detection based on a diode
array design from 190 – 950 nm. A temperature management system make the
optical design now even more tolerant to ambient temperature changes.
• Accelerate your analytical observations by monitoring analytes
and impurities simultaneously.
• Acquire up to 5 signals at different wavelengths during a single analysis -
each optimized for a specific analyte and therefore highest sensitivity.
• Optimize selectivity and sensitivity with the programmable slit.
• Upgrade later to full spectral capability.
You can perform sensitivequantitation of active compoundsand impurities in a single runwithout wavelength switching.
1024-elementdiode-array
Tungsten lamp
Deuterium lamp
Acromaticlens
Holmiumoxide filter
Standardflow cell
Programmable slit
190 nm
950 nm
Time [min]2 4 6 8 10 12 14 16 18
mAU
0
500
1000
1500
2000
2500
3000
254 nm
370 nm
204 nm
Quercetin
Isorhamnetin
Kaempferol
Active compoundsGinko Biloba extract
The longlife(> 2000 hours)
deuterium lampsaves operating
costs !
Agilent 1100 Series multiwavelength detector–for more information and flexibility without any loss in sensitivity
The Agilent 1100 Series diode-array detector is designed for highest optical
performance and reliability, more than 10,000 users have decided on a detector that
offers an astonishing set of features.
• Expand your detection range with the deuterium and tungsten lamps, offering
190 to 950 nm with highest intensity and lowest detection limit.
• Use the power of 1024 diodes and the 1-nm slit for high spectral resolution.
• Optimize sensitivity, linearity and spectral resolution rapidly with the programmable
slit (1 to 16 nm). A motor allows you to program the slit as part of your method for
optimum incident light conditions. There is no need for separate documentation.
• Verify separation quality with peak purity, multiple signal and spectral libraries—
interactively or automatically in the same run as your analytical quantification.
• Use a reference wavelength to eliminate background interferences.
Agilent 1100 Series diode array detector–for complete confidence from spectral data
Do capillary HPLC with the500-nanoliter flow cell to improvedetection limits 50 to 100-foldcompared to standard-bore HPLC
Twin lamps design lets youovercome the sensitivity limitsof conventional detectors in thevisible range.
Independent control of lampsensures maximum lifetime.
Time [min]20 30 40 50 60 70
mAU
0
100
200
300
400
500
600 Sample: 10 pmol of tryptic digest of horseskeletal muscle myoglobin
Column: 300 µm id, 25 cm length,ZORBAX 300SB-C18, 5 µm
Flow rate: 5.5 µl/minSolvent: A: 0.05 % TFA in water
B: 0.045 % TFA in acetonitrileGradient: 1 – 61 % B in 120 minutes
Typical peak width
0.025 min
0.05 min
0.1 min
0.2 min
Typical column length
T <= 5 cm
10 cm
20 cm
>= 40 cm
Recommended flow cell
Typical flow rate 0.01 – 0.2 ml/min 0.2 – 0.4 ml/min 0.4 – 0.4 ml/min 1– 2 ml/min 0.01 – 5 ml/min
High-pressureflow cell
For pressures above 100 bar
Internal column diameter 0.3 – 1 mm 2.1 mm 3.0 mm 4.6 mm
500 nl flow cell
Semi-microflow cell
Standard flow cell
The 500 nanoliterflow cell assuresminimized dispersionthrough its optimizedgeometry
Quantitative and qualitative information intrace analysis.
The optics are designed to provide highestsignal-to-noise ratios and a large dynamicrange. Together with sophisticated spectralsearch and compare algorithms, match factorsare excellent—even for trace-level spectrawith less than 1 mAUFS.
You can focus on fine bands withthe narrow slit setting.
Spectral resolution depends on slitwidth, here just 1 nm. At this settingthe fine structure of benzene can berevealed—even at 0.7 mAUFS.
240 320260 280 300 340 360 380
Wavelength [nm]
Benzo(g,h,i)peryleneStandard, 725 mAUFS
DAD 1, 21.801Sample, 1.1 mAUFS
Match factor: 988
Absorbance[normalized]
1 nm slit
0.60.50.40.30.20.1
0
1 nm
2 nm
4 nm
8 nm
0.7 mAU
230 240 250 260 270 280 nm
A temperaturemanagement system
makes the proven optical designnow even more tolerantto ambient temperatures
changes.
Built-in tools that makeyour work easier and faster
Setting up the analytical method
System features such as an intuitivegraphical user interface, context-sensitivehelp and an online tutorial save operatortraining time. You can use the AgilentChemStation’s color-coded system displayor the cue card-like screens of thehandheld control module to enter alldetector parameters easily. You can storemethod information on a PC card in thehandheld controller or on the computer’shard disk for GLP compliance. Thisincludes all instrument parameters, evenslit settings.
Validating the HPLC detector
Built-in automated tools for installationand performance verification prove thesuitability of the complete Agilent 1100Series HPLC system or the standalonedetector. This makes it easy to complywith regulatory and quality standards thatrequire routine verification of wavelengthaccuracy for UV-visible detectors. Resultscan be stored on a disk for easy review.A qualification workbook and standardoperating procedures for such activitiesas wavelength verification are available.
1. 2.
Reviewing results immediately
You can monitor separation progressonline with the handheld control moduleor the Agilent ChemStation. Moreover, theAgilent ChemStation lets you view onlineplots of multiple signals and spectral dataor take snapshots for evaluation while therun is underway.
Combine the ChemStation with Agilent'sSecurity Pack and you get FDA 21 CFRPart 11 compliance for electronicsignatures and records.
Automating quality assurance
You can preset chromatographic qualitycontrol criteria for automated qualityassurance. If the system does not passyour preset criteria, the built-in systemsuitability software alerts you and can stopthe system before more time and samplesare wasted. You can alsodefine a lamp burn-timelimit to prevent qualitycontrol failures.
4.
5.Saving costs of ownership
Time-programming, early maintenancefeedback (EMF) and diagnostic softwarehelp to prevent costly instrument down-time. You can preserve the lamp for alonger productive life with automaticshutdown after analysis, or use clocktimeprogramming to switch on the lamp andeliminate waiting periods at the start of theday. Pre-aligned for fast, error-freeexchange, the lamp is easy to install fromthe front.
The built-in diagnostic software lets youidentify sources of chromatographicproblems, such as a contaminated flowcell or failing lamp.
3.
Spectral landscapes that enable faster sample characterization–routinely
The Agilent 1100 Series industry-standard
diode array detector has made its way
into routine laboratories because of its
sensitivity, ease of use and automated
features for spectral analysis. Now you
can quantify and confirm the identity of
analytes at the lowest trace-level amounts
in a single analysis.
A bird’s eye view speeds up wavelength optimizationand extraction of additional signals.
You can view the data from above and follow thecontours of an analyte’s isoabsorbance plot. Slidingthe cursor through the spectral landscape lets youfind the best signal response.
With the Agilent 1100 Series diode arraydetector:• A single method encompasses both
quantitative and spectral routines forpeak purity and peak confirmation.
• A variety of automatic spectralacquisition modes (all-spectra-in-run,peak-controlled, slopes and apex) areoptimized for minimum file size. Collectonly the wavelength range you wantand it prevents your PC’s hard diskfrom overflowing during unattendedoperation, even in high-throughputapplications.
• Spectral libraries and sophisticatedsearch routines support interactive orautomatic compound confirmation.
• Impurity detection routines, such aspeak purity algorithms, are advancedenough to detect irregularities at thefront and tail of a peak—interactively orautomatically, in research and qualitycontrol.
• Report templates let you present signaland spectral data tailored to corporate,regulatory or client standards, automati-cally.
Additional signals can be extractedand added to the existing method forquantitative data analysis.
An interactive online Show-me tutorialdemonstrates step-by-step the simple routeto peak purity.
You can take the simple route, or explore thedepths with spectra similarity routines.The Agilent 1100 Series diode array detectorcombined with the Agilent ChemStation canreveal impurities as small as 0.1 percent—evenwhen coeluting at the beginning or end of anotherwise well-resolved peak.
You can create your own library ofspectral standards and rapidly map anunknown compound’s spectrum.
The Agilent ChemStation lets you screenunknowns automatically. Interactively orunattended.
Various tools give you fast setup.
The Agilent ChemStation’s graphical userinterface makes it fast and simple to set upand run standards and store method-specificspectra together with search criteria. OnlineShow-me tutorials can greatly reduceoperator training time.
Combining search criteria enhances certainty.
Applying reference spectra, averaged referencespectra, retention time windows, smooth factorsand absorbance thresholds refines your searchprocedure, for the best match.
Copyright © 2001 Agilent Technologies
The information in this publication is subject tochange without notice.
All Rights Reserved. Reproduction, adaptation ortranslation without prior written permission isprohibited, except as allowed under the copyrightlaws.
Printed in Germany, October 1, 2001Publication Number 5980-1532E
SpecificationsAgilent 1100 Series UV-visible Detectors
www.agilent.com/chem
Detector type:
Light source:
Short-term noise:*
Drift:*
Linearity:
Wavelength range:
Wavelength accuracy:
Slit width:
Diode width:
Wavelength bunching:
Flow cells: • Standard
• Semi-micro
• Micro
• Nano
� High pressure
Time programmable:
Spectral tools:
Analog output:
Specials:
Variable wavelength detector
Double beam
Deuterium lamp
± 0.75 × 10-5AU at 254 nm
1 × 10-4 AU/h at 254 nm
> 2 AU upper limit
190 – 600 nm
±1 nm, self-calibration with deuteriumlines, verification with Holmium oxidefilter
6.5 nm typical
not applicable
not applicable
14 µl, volume, 10 mm cell path length40 bar (588 psi) pressure maximum5 µl, volume, 6 mm cell path length40 bar (588 psi) pressure maximum1 µl, volume, 5 mm cell path length40 bar (588 psi) pressure maximumThird party, contact Agilent for details
14 µl, volume, 10 mm cell path length400 bar (5880 psi) pressure maximum
Wavelength, polarity, peak width,lamp on/off
Stop-flow wavelength scan
Recorder/Integrator 100 mV or 1 V,1 output
Optional cuvette holder
Multiwavelength detector Diode array detector
1024-element diode array 1024-element diode array
Deuterium and tungsten lamp Deuterium and tungsten lamp
±1.0 x 10-5 AU at 254 nm and at 750 nm ±1.0 × 10-5 AU at 254 nm and at 750 nm
2 x 10-3 AU/h at 254 nm 2 × 10-3 AU/h at 254 nm
> 2 AU upper limit > 2 AU upper limit
190 – 950 nm 190 – 950 nm
±1 nm, self-calibration with deuterium lines, ±1 nm, self-calibration with deuterium lines,verification with Holmium oxide filter verification with Holmium oxide filter
Programmable: 1, 2, 4, 8, 16 nm Programmable: 1, 2, 4, 8, 16 nm
< 1 nm < 1 nm
Programmable: 2 to 400 nm, in steps of 1nm Programmable: 2 to 400 nm, in steps of 1nm
13 µl, volume, 10 mm cell path length 13 µl, volume, 10 mm cell path length120 bar (1760 psi) pressure maximum 120 bar (1760 psi) pressure maximum5 µl, volume, 6 mm cell path length 5 µl, volume, 6 mm cell path length120 bar (1760 psi) pressure maximum 120 bar (1760 psi) pressure maximum1.7 µl, volume, 6 mm cell path length 1.7 µl, volume, 6 mm cell path length400 bar (5880 psi) pressure maximum 400 bar (5880 psi) pressure maximum500 nanoliter, volume, 10 mm cell path length 500 nanoliter, volume, 10 mm cell path length40 bar (588 psi) pressure maximum 40 bar (588 psi) pressure maximum1.7 µl, volume, 6 mm cell path length 1.7 µl, volume, 6 mm cell path length400 bar (5880 psi) pressure maximum 400 bar (5880 psi) pressure maximum
Wavelength, polarity, peak width, Wavelength, polarity, peak width, lamp on/offlamp on/off bandwidth, autobalance bandwidth, autobalance
WL range, threshold, spectra storage mode
Stop-flow wavelength scan Data analysis software for spectra evaluation,including spectral libraries and peak purityfunctions
Recorder/Integrator 100 mV or 1 V, 2 outputs Recorder/Integrator 100 mV or 1 V, 2 outputs
Specifications common to all detectors
Control: Parameter entry, multi-signal display, online help and diagnostics on the Agilent 1100Series control module, optional PC (PCMCIA) card for method, sequence and logbookstorage. Agilent ChemStation for HPLC as computer platform for control and dataevaluation.
Communications: Controller-area network (CAN), HP-IB, RS-232C, APG.Remote outputs: ready signal. In- and outputs: start, stop and shutdown signals
Safety and Extensive diagnostics, error detection and display (through control module andmaintenance: Agilent ChemStation), leak detection, safe leak handling, leak output signal for
shutdown of pumping system. Low voltages in major maintenance areas.GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in
terms of lamp burn time with user-settable limits and feedback messages. Electronicrecords of maintenance and errors. Verification of wavelength accuracy with built-inHolmium oxide filter.
Housing: All materials recyclableEnvironment: 0 to 55 °C constant temperature at < 95 % humidity (non-condensing)Dimensions: (h × w × d) =140 mm × 345 mm × 435 mm, (5.5 × 13.5 × 17 inches)Weight: 11.5 kg (25.5 lbs)
*According to ASTM E1657-96