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HPA Culture Collections News & Product Update...
Spring/Summer 2011
WIN!
HPA Culture CollectionsA strategic business unit within the Health Protection Agency.
www.hpacultures.org.uk Tel: +44 (0) 1980 612512 email: [email protected] k
HPA Culture CollectionsA strategic business unit within the Health Protection Agency.
www.hpacultures.org.uk Tel: +44 (0) 1980 612512 email: [email protected] k
Products:
• Cell Lines and Hybridomas• Primary Cells• Neuron Culture Kits• DNA and RNA Products• Bacteria, Plasmids, Transposons• Mycoplasmas• Fungi• Lenticule Discs• Viruses
• Contract Freeze-Drying• Mycoplasma Testing and
Eradication• NCPV Contract Services• Patent Deposits• Safe Deposits• Sterility Testing• Training
Services:
• Cell Culture Management Services
• Contract Cell Culture• Custom Genetic
(Cell Lines)• Assay Ready Cells• Cell Line Identity • DNA Extraction• Genetic Support Services
Products and Services available from the HPA Culture Collections: Visit www.hpacultures.org.uk
WIN!Another chanceto win an iPodshuffle in your choiceof colour!
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Are you
testingfor it?
New Cell Culture Training Course • Mycoplasma and Authentication Workshops • EVA Free Virus Sample Initiative • 2nd Edition Laboratory Handbook Out Now
New ECACC Fundamentals of Cell Culture Training CourseSee inside for more...
Electron micrograph
of M
ycop
lasm
a
Down1- Narcotic painkiller (8)3 - A surface on which an organism grows (9)4 - Hot, used to spice up curries (6, 6)6 - RNA and DNA (7, 4)8 - Disease characterised by reduced bone density (12)
Across2 - Death of cells caused by external factors (8)4 - Final section of the digestive tract (5)5 - Skin cancer (8)7 - The place where something begins (6)9 - Maintains pH balance (6)10 - These like it hot! (12)13 - Cells, allow perception of colour (5)17 - Expressed region of a gene (4)18 - Inadequate oxygen supply (7)19 - Chewing (11)20 - Proteins around which DNA winds to become compact (8)
of colour:Use the letters in the orange squares to form a ten letter word (clue: the word is related to a testing service available from the Culture Collections):
_ _ _ _ _ _ _ _ _ _
Then visit our website at the crossword entry page www.hpacultures.org.uk/promotions/crossword.jsp and submit your answer. Closing date 31st July 2011
12 - Some use Viagra to get one of these (8)14 - Exocrine gland of the male reproductive system (8)15 - Undergo a change (6) 16 - Back bone (5)
11 - Loves water (11)
New Edition Out NowFundamental Techniques
Laboratory2nd Edition
To receive your www.hpacultures.org.uk/
in Cell Culture
Handbook
FREE copy, please visit
promotions/labhandbook.jsp
Workshop Success: Misidentified Cell Lines
Mycoplasma and
Are you testing for it? Oesophageal Cancer Cell LinesNEW
A series of (o)esophageal adenocarcinoma (EAC) cell lines are in the process of being added to the ECACC catalogue. This follows the publication of a study by Boonstra et al., 2010, who in a world-wide effort sought to verify the authenticity of all available EAC cell lines by comparing the cell lines to the original primary tumour tissue from the donors.
Boonstra et al. discovered that cell lines SEG-1, BIC-1, and SK-GT-5 are not EAC cell lines but large cell lung cancer cell line H460, colorectal adenocarcinoma cell line SW620, and gastric fundus carcinoma cell
that cell lines FLO-1, KYAE-1, SK-GT-4, OE19, OE33, JH-EsoAd1, OACP4C, OACM5.1, ESO26, and ESO51 are derived from human EACs. All of these 10
information, have been deposited with ECACC.
Cell Line FLO-1
Used Human Esophageal Adenocarcinoma Cell Lines. J Natl Cancer Inst. Feb 24;102(4):271-4.
Limited O�er!
FREE access to virus strains
Enabled by European Virus Archive
To apply, please visit www.hpacultures.org.uk/ncpv/evao�er.jsp
Snapshot presentations of novel Mycoplasma detection techniques from Lonza (MycoAlert™) and Life Technologies’ (Myco Seq™) were given. An extended lunch break included a tour of the ECACC Mycoplasma and Molecular Biology laboratories.
The requirement for biological standards in Mycoplasma testing and cell line authentication, particularly with regard to the manufacture of novel cell based therapeutics was discussed. A specialist responsible for the culturing of newly acquired cell cultures, gave a fascinating insight into
contaminated and Mycoplasma infected cell lines. This included some thought provoking data from experiments that simulated accidental low dose inoculation of cells into typical cell culture reagents, the surprising longevity of these cells and their ability to be recovered and cultured from these reagents after weeks of cold storage.
The workshop closed with a summary of conclusions taken from the discussions. These conclusions are well worth reading and can be viewed as part of the detailed write up of the proceedings on our website at: www.hpacultures.org.uk/services/training/
View the full write up of the proceedings, speakers and summary conclusions on our website!
mycoplasmaonedayworkshop.jsp.
The two major risks facing any practitioner of cell
of cell cultures with Mycoplasma were explored in a new one day workshop hosted at ECACC this March. Due to the success of the workshop, attracting delegates from academic and commercial organisations from
.tneve launna na emoceb ot sesimorp ti ,eporuE ssorca
ECACC would like to thank all those who presented which included internal specialists from the HPA Culture Collections and external speakers such as Professor Glyn Stacey and Lyn Healy from the UK National Stem Cell Bank (NSCB), Ross Hawkins from NIBSC and representatives from Lonza and Life Technologies.
The day began with a key note introduction to Good Cell Culture Practice (GCCP) guidelines, the history of cell
portfolio of cell line authentication techniques has evolved
Subsequently a focused appraisal of Short Tandem
including how to select a set of primers and the issues
laboratory. Developments at ECACC were presented such as the recent introduction of DNA bar-coding to replace
The emphasis then shifted to Mycoplasma beginning with a detailed review of the biology and pathology of the Mycoplasma genus from the perspective of a microbiologist.
FREE Sample
To receive a free sample submit your request online
at: www.hpacultures.org.uk/products/lenticulediscs/
freelenticulediscsample.jsp
HPA Culture CollectionsA strategic business unit within the Health Protection Agency.
www.hpacultures.org.uk Tel: +44 (0) 1980 612512 email: [email protected]
Key Benefi ts of NCTC LENTICULE® Discs:Quantitative NCTC LENTICULE® discs are an easy to use source of reproducibly quantifi ed, biologically active microorganisms (bacteria & fungi) in a solid, water-soluble matrix.
To view the complete listing & order online – visit www.hpacultures.org.uk
FREE SAMPLE To receive a free sample submit your request online at: www.hpacultures.org.uk/products/lenticulediscs/freelenticulediscsample.jsp
Quantitative reference material for microbiology NCTC LENTICULE® discs Easy to use reference materials for your routine internal quality control systems such as water, food and environmental testing. Registered to ISO 9001: 2008
Original NCTC strains traceable, a single subculture from source
Cost effective25 discs per pack, each disc in an individual vial
Convenient take a new disc each time, avoid the pitfalls of serial propagation and burden of maintaining your own stocks
Easy to Use apply direct to solid or liquid media
Key quality control strains for water, food and environmental testing
Reference Standard suitable to aid compliance with ISO 17025
High & low count formats available
NCTC Discs
Quantitative reference material for microbiology
Lenticule
NCTC LENTICULE® discsQuantitative reference material
for microbiology
HPA Culture CollectionsA strategic business unit within the Health Protection Agency.
www.hpacultures.org.uk
• Original NCTC strains
• Convenient
• High & low count formats
• Reference Standard
• Cost Effective
E.coli
HPA Culture CollectionsA strategic business unit within the Health Protection Agency.
www.hpacultures.org.uk Tel: +44 (0) 1980 612512 email: [email protected]
Key Benefi ts of NCTC LENTICULE® Discs:Quantitative NCTC LENTICULE® discs are an easy to use source of reproducibly quantifi ed, biologically active microorganisms (bacteria & fungi) in a solid, water-soluble matrix.
To view the complete listing & order online – visit www.hpacultures.org.uk
FREE SAMPLE To receive a free sample submit your request online at: www.hpacultures.org.uk/products/lenticulediscs/freelenticulediscsample.jsp
Quantitative reference material for microbiology NCTC LENTICULE® discs Easy to use reference materials for your routine internal quality control systems such as water, food and environmental testing. Registered to ISO 9001: 2008
Original NCTC strains traceable, a single subculture from source
Cost effective25 discs per pack, each disc in an individual vial
Convenient take a new disc each time, avoid the pitfalls of serial propagation and burden of maintaining your own stocks
Easy to Use apply direct to solid or liquid media
Key quality control strains for water, food and environmental testing
Reference Standard suitable to aid compliance with ISO 17025
High & low count formats available
Fundamentals of Cell Culture 18th - 21st October 2011
ECACC Training Course
• Solutions for dealing with cellcultures at various scales
• Problems and trouble-shooting
• Cell culture application
• Application based practicalexperiments
• Cell banking subculture• Selective culture and cloning• Primary cell culture techniques• Cell line • Strategies of identity testing• Detection of microbial
contaminants inc. Mycoplasma
NEW!
• Origins and principles of cell culture
• Qualitative characteristics of cell cultures
• Good Cell Culture Practice (GCCP)
• Cell counting and analysis• Cryopreservation
ECACC are pleased to announce a new course for autumn 2011: ‘The Fundamentals of Cell Culture’. This 4 day course has a balance of cell culture theory, classrooom based workshops together with a strong emphasis on practical skills. For more than twenty years ECACC has been providing cell culture training; the content and format has been continually adapted and improved in response to the needs of end users. This new course includes the following:
We are pleased to announce that the renowned author and cell culture expert R. Ian Freshney PhD will be delivering the foundation lectures on this course.
Course price: £1,680 per delegate or £1,400 (20% early bird discount) for registering on or before the 31st August 2011. To book, please contact +44 (0)1980 612 773 or at [email protected].
