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HPA Culture CollectionsA strategic business unit within the Health Protection Agency.
www.hpacultures.org.uk Tel: +44 (0) 1980 612512 email: [email protected]
HPA Culture Collections News & Product Update...
Topics include:• The cell culture laboratory and equipment overview• Locker area and entry into the laboratory• The microbiological safety cabinet and its use• Revival of cells from frozen• Cell growth to confl uence• Subculture• Counting cells (Trypan blue)• Cell freezing & banking• Envrionmental and routine sterility testing
The fi lm can be viewed from start to fi nish and then individual sections can be re-visited separately as required. Subtitles are available in French and German.
To apply for a free copy of the DVD (rrp £30) – please submit your request on our website, visit page: www.hpacultures.org.uk/promotions/cellculturedvd.jsp
Cell Culture Training Courses at ECACC
In recognition that there is no substitute for practical cell culture experience ECACC offers specially developed cell culture courses consisting of a mixture of lectures and practical training. The Level 1 Cell Culture Training Course is designed to introduce newcomers to cell culture and the Level 2 Course caters for the experienced cell biologist with more specialised requirements.
New Training DVD Released • Back-Up Your Stocks - Storage Offer • New Cell Lines • Genetic Interventions Meeting • NCPV Awarded Funding
WIN!an i-pod shuffl e
NEW - Cell Culture Training DVD – Free to fi rst 100 applicants
The European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich has produced a cell culture training DVD ‘A Guide to Successful Cell Culture’. The DVD is a 30 minute fi lm demonstrating and describing key areas of cell culture fi lmed on
location at ECACC.
Spring 2009
For more information on the next available dates, prices and course content – visit
www.hpacultures.org.uk
Back-Up Your Stocks – Secure Storage
Second site Ultra Low Temperature storage (below -135oC) for your valuable research or production cell banks. Take advantage of ECACC’s newly launched storage format - individual safe deposit box service.
Benefi ts:• Only your cells stored in your own personal
confi dential safe box • Each box holds up to 100 x 1ml cryovials • Larger vials can be stored in the box but
capacity will be reduced • Service includes 2 free transactions per
year i.e. addition or removal of any number of ampoules up to the capacity of the box*
• Security guaranteed through restricted access site
• Full monitoring service including annual report of the testing of 3 monitor cell lines
• Human Tissue Authority (HTA) licensed cGMP compliant validated tank service also available
Price of Service:£750 per box per year for the fi rst 4 boxes £500 per box for 5+ boxes
Introductory Offer Get a 30% discount on the above for the fi rst year if you reply by 30th April 2009 using the code ‘Boxit0409’. If you are a new customer we will also waive our new customer set up fee of £225**
For £500 per year secure your valuable cell stocks now before it’s too late………..
* delivery and packing charges excluded
** cGMP service set-up fee excluded from this offer
ETCS One Day Meeting - Genetic Interventions in Cell Culture; Technologies & Applications
Date: 1st April 2009 Venue: University College London
The European Collection of Cell Cultures (ECACC) in collaboration with the European Tissue Culture Society (ETCS) is presenting a one-day meeting on the recent developments in the technologies in genetic modifi cation of cells in culture and their application. Target audience: This one-day course is aimed at scientists in academic and commercial organisations who require an updated review of techniques for genetic manipulation of mammalian and insect cell lines in culture and their current application.
Content:The content will provide attendees with a current overview of gene transfer technologies in development and their use in drug discovery, clinical applications or studies in basic cell biology. Topics include:
• New transfection technologies (cationic liposomes and electroporation)
• Viral delivery technologies (Retrovirus, Lentivirus & Adenovirus) • New advances in Baculovirus systems• Genetic Knock-ins, Knock-outs and animal stem cells systems• Post-transcriptional silencing (siRNA) in cancer research• Precise genetic manipulation and regulation of transgene expression • Cell Immortalization• Cell line modifi cation for assay development for Drug discovery
(High-throughput screening)
Price & How to Register: Visit the HPA Culture Collections website www.hpacultures.org.uk/events/ETCSGeneticInterventionsApril09.jsp
NCPV Awarded European Funding to Expand Virus Collection
The National Collection of Pathogenic Viruses (NCPV) has been established for 10 years and has steadily built-up a good reputation as an internationally recognised Biological Resource Centre (BRC). The virus collection is primarily based on viruses sourced from within the UK and is focussed
on human pathogens.
We have long recognised that there are good virus collections held by many European institutions that are not well catalogued and are not generally available to the scientifi c community. In light of this, NCPV has recently
started to work with a range of European
www.hpacultures.org.uk Tel: +44 (0) 1980 612512 email: [email protected]
partners with the aim of establishing a European Virus Archive (EVA), and has obtained EU funding to enable this process.
The initial ‘kick-off’ meeting for EVA was in February 2009 and, over the next few years, it is hoped that an extensive range of interesting viruses will become available to researchers. NCPV operations will continue and expand as before, but if customers are unable to obtain viruses required via the NCPV catalogue, then hopefully by utilising the EVA network then these omissions can be fulfi lled.
Visit www.hpacultures.org.uk to view the NCPV Virus collection. If you can’t fi nd the virus you are looking for – contact us so we can look to obtain a deposit.
New Operations Manager at NCTC
In September 2008 Steven Brimble was appointed Operations Manager for the National Collection of Type Cultures (NCTC), part of the Health Protection Agency Culture Collections.
Previously Steve worked as Quality Manager at the Health Protection Agency, Centre for Infections, Colindale liaising with staff in the Department of Healthcare Associated Infections and Antibiotic Resistance, Molecular Identifi cation Service Unit, Applied and Functional Genomics Unit and the NCTC. Before this Steve worked for the Food and Environmental Profi ciency Testing Unit, FEPTU, producing freeze dried bacterial cultures for EQA schemes aimed at food and water testing laboratories.
The NCTC has over 5000 bacterial cultures, together with over 100 mycoplasmas, more than 500 plasmids, host strains, bacteriophages and transposons available for supply. NCTC also provides a range of associated services, which include freeze-drying of customers’ own strains. Furthermore, the NCTC is an International Depository Authority (IDA) patent depository for bacterial cultures. Visit www.hpacultures.org.uk to view the complete listing of products and services available from NCTC.
See back cover for your chance to win one of 3 i-pod shuffl es! > > > >
WIN!
Want to save time? Use Assay Ready Cells to Accelerate Drug Discovery
ECACC supplies frozen “Assay Ready” cells for drug discovery applications as part of our Cell Culture Management Services. This allows customers to decouple cell culture preparation from high throughput screening programmes, providing cells “off the shelf” to customer specifi cations when they are needed, and in the quantities required. Benefi ts include:
• No more need for continuous cell culture• Screen 5 days a week• Run consecutive primary and secondary
screening• Return to the same, standardized cellular
material whenever you want• Fully confi dential service
Provide us with a fl ask or an ampoule of the cell line you wish to use, e.g. cells expressing your protein of interest, and we will manufacture cell banks to your specifi cation, using one of a range of large scale cell culture systems.
A typical Assay Ready Bank consists of 100 to 500 ampoules, each containing 1 x 107 or more cells, cryopreserved in a programmable rate controlled freezer and certifi ed free of microbial (including mycoplasma) contamination.
Contact us NOW to discuss your needs.
New Primary Cell Types Available!
Over 100 Primary cell types plus specialised media are available, recent additions include:
Primary Cell TypesCell Line Name Description Cat. No.HBcAEC (3012-05a) Human Brachiocephalic Artery Endothelial Cells; adult NEW 07073103HCtAEC (3014-05a) Human Carotid Artery Endothelial Cells; adult NEW 07073104HBcASMC (3512-05a) Human Brachiocephalic Artery Smooth Muscle Cells; adult NEW 07073106HCtASMC (3514-05a) Human Carotid Artery Smooth Muscle Cells; adult NEW 07073107HPAd (S802s-05a) Human Preadipocytes; subcutaneous; pre-screened NEW 09010907HORSC (606-05a) Human Hair Follicle Outer Root Sheath Cells NEW 07073109 HNSC (HS820-20f) Human Neural Stem Cells; foetal NEW 07073111HCF (306-05a) Human Cardiac Fibroblasts; adult NEW 09010901HMSC (492-05f) Human Marrow Stromal Cells; foetal NEW 07073108More………………………………… visit www.hpacultures.org.uk to view the entire Primary Cell Collection
HPA Culture CollectionsFour Collections - ONE SOURCE
www.hpacultures.org.uk
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www.hpacultures.org.uk Tel: +44 (0) 1980 612512 email: [email protected]
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WIN!New Cell Lines – ECACC’s Cell Line Collection Continues to Expand…………
Several hundred new cell lines have been added to our cell line collections since the publication of the last ECACC hardcopy catalogue (dated 2005/06). Here we list a selection of these new products for your reference. Our collection is continually expanding; visit www.hpacultures.org.uk for the most up-to-date listings.
New Neuronal Cell Lines
More than 50 New Neuronal cell lines recently added to the collection, examples include:Cell Line Cat no. Species Tissue CommentsANGM-CSS 08040401 Human Brain Human glioblastoma
NH15-CA2 08062525Mouse x rat
BrainHybrid of mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid line 108CC15 and the mutant mouse neuroblastoma line N115-BU-8
C6-2-3 08062528 Rat BrainA pseudotetraploid cell line generated by fusion of 8-bromodeoxyuridine-resistant rat glioma C6-BU-1 and the 6-thioguanine-resistant rat glioma C6-TG-1
C6-4-2 08062524 Rat BrainPseudohexadecaploid cell line generated by fusion of the 8-bromodeoxyuridine resistant rat glioma C6-3-BU-8 and the 6-thioguanine-resistant rat glioma C6-3-TG-3
C6-BU-1 08062563 Rat Brain A 8-bromodeoxybromidine resistent mutant of rat glioma cell line C6C17.2 07062902 Mouse Cerebellum Mouse multipotent neural progenitor or stem-like cellsF11 08062601 Rat Embryo, DRG Rat embryonic dorsal root ganglionCHP-134 06122002 Human Neuroblastoma Human neuroblastoma tumour mass of left adrenal glandLA1-55n 06041203 Human Neural Human NeuroblastomaLA1-5s 06041204 Human Neural Human Neural Crest-Derived Non-Neuronal ProgenitorLA-N-1 06041201 Human Neuroblastoma Human Neuroblastoma Bone Marrow MetastasisLA-N-2 06041202 Human Neuroblastoma Human NeuroblastomaN115-BU-1 08062547 Mouse Neuroblastoma Bromodeoxyuridine-resistant mutant of mouse neuroblastoma N1E-115
More clones related to N115-BU-1 available e.g. N115-BU-2, N115-BU-7, N115-BU-8, N115-BU-9, N115-BU-10
N18 08062527 Mouse Neuroblastoma A clone of mouse neuroblastoma C1300 cell lineN18TG2 08062523 Mouse Neuroblastoma A 6-thioguanine resistant clone of N18N1E-115-1 08062511 Mouse Neuroblastoma Mouse (A/jax) neuroblastomaN4TG3 08062515 Mouse Neuroblastoma Mouse (A/jax) neuroblastomaNS20Y 08062517 Mouse Neuroblastoma Mouse (A/jax) neuroblastomaNS20Y-BU-2 08062502 Mouse Neuroblastoma Mutant of cholinergic cell line NS20Y derived from neuroblastoma C1300
More clones related to NS20Y-BU-2 available e.g. NS20Y-BU-2, NS20Y-BU-4, NS20Y-BU-5, NS20Y-BU-6, NS20Y-BU-7
NS20Y-TG 08062512 Mouse Neuroblastoma Mouse (A/jax) neuroblastomaCHP-100 06122001 Human Neuroblastoma Human neuroblastoma epidural tumour of spineMore available……visit www.hpacultures.org.uk to view the complete listing…….
New Urology Related Cell Lines
14 New Urology cell lines recently added to the collection, examples include:Cell Line Cat no. Species Tissue CommentsU-BLC1 06013102 Human Bladder Human bladder transitional cell carcinomaUM-UC-1 06080301 Human Bladder Human bladder transitional cell carcinomaUM-UC-13 08090508 Human Bladder Human transitional cell carcinoma lymphatic metastasisUM-UC-4 08090501 Human Bladder Human adenocarcinoma lymph node metastasisUM-UC-5 08090502 Human Bladder Human squamous cell carcinoma of the bladder
UM-RC-2 08090511 HumanKidney-renal gland
Human renal carcinoma
UM-UC-14 08090509 Human Renal pelvis Human transitional cell carcinoma of the renal pelvis
More available……visit www.hpacultures.org.uk to view the complete listing…….
New Human Head & Neck Cancer Cell Lines
17 New Head & Neck Cancer cell lines recently added to the collection, examples include:Cell Line Cat no. Species Tissue CommentsBICR 10 04072103 Human Buccal mucosa Buccal mucosa squamous carcinomaBICR 16 06031001 Human Tongue Tongue squamous carcinomaBICR 18 06051601 Human Larynx Larynx squamous cell carcinoma (lymph node metastasis)BICR 22 04072106 Human Tongue Tongue squamous carcinoma (lymph node metastasis)BICR 82 04072112 Human Jaw (maxilla) Human Upper Jaw (Maxilla) CarcinomaH314 06092003 Human Mouth, floor of Human oral squamous cell carcinoma, floor of mouthH357 06092004 Human Tongue Human oral squamous cell carcinoma, tongueH400 06092006 Human Alveolar process Human oral squamous cell carcinoma, alveolar processH413 06092007 Human Buccal mucosa Human oral squamous cell carcinoma, buccal mucosa
www.hpacultures.org.uk Tel: +44 (0) 1980 612512 email: [email protected]
More available.........visit www.hpacultures.org.uk to view the complete listing.......
www.hpacultures.org.uk Tel: +44 (0) 1980 612512 email: [email protected]
More New Cell Lines added to the ECACC General Cell Collection - since last hardcopy catalogue, visit our website for the most up-to-date listings
Cell Line Cat no. Species Tissue CommentsCP5 08052101 Bovine Articular cartilage Bovine, Bos taurus (Holstein-Fresian), articular cartilage progenitorKARPAS 231 06120601 Human B cell leukemia Human B-cell leukemiaKARPAS 422 06101702 Human B cell lymphoma Human B cell non-Hodgkin lymphomaKARPAS 384 06120602 Human Blood Human T-cell non-Hodgkin lymphomaKARPAS 1106P 06072607 Human Blood Human B-Cell Non-Hodgkin’s LymphomaKARPAS 45 06072602 Human Bone marrow Human T-cell LeukaemiaKARPAS 299 06072604 Human Lymphoma Human Non-Hodgkin’s Ki-positive Large Cell LymphomaKARPAS 417 06100302 Human Myeloma Human myelomaVP229 05092804 Human Breast Human Breast CancerVP267 05092805 Human Breast Human Breast CancerVP303 05092806 Human Breast Human Breast CancerHCA-2 06061901 Human Colon Human sigmoid colon adenocarcinomaHCA-24 06061903 Human Colon Human colon adenocarcinomaHCA-46 07031601 Human Colon Human colon adenocarcinomaHCA-7 06061902 Human Colon Human colon adenocarcinomaCGR8 07032901 Mouse Embryo Mouse embryonic stem cellSNL 76/7 07032801 Mouse Embryo Mouse SIM strain embryonic fibroblast
1156QE 06072609 HumanEmbryonal carcinoma
Human embryonal carcinoma
833KE 06072611 HumanEmbryonal carcinoma
Human embryonal carcinoma
577MF 06011802 HumanForehead, teratoma
Human forehead metastasis of malignant teratoma
1411H 06011805 Human Germ cell tumour Human yolk sac carcinoma1777N Rpmet 06011801 Human Germ cell tumour Human embryonal carcinomaN2102Ep Clone 2/A6
06011803 Human Germ cell tumour Human embryonal carcinoma
MDCK-SIAT1 05071502 Dog Kidney Canine Cocker Spaniel Kidney Sialic Acid Over ExpressionA9 08062526 Mouse L cell fibroblast Mouse C3H/An fibroblasts, 6-thioguanine resistant B82 08062522 Mouse L cell fibroblast Mouse C3H/An fibroblasts, 8-bromodeoxyuridine resistantC22 (Clara) 07021401 Mouse Lung Mouse lung epithelial progenitor cellT7 07021402 Mouse Lung Mouse Type-II lung epithelial cellMT-2 08081401 Human Lymphocyte Human T cellMT-4 08081402 Human Lymphocyte Human T cellREM 885 06070401 Human Lymphocyte Human EBV transformed lymphocytes Alzheimer
COV413A 07071905 HumanOvarian metastasis
Human ovarian epithelial-serous carcinoma, sigmoid
COV413B 07071906 HumanOvarian metastasis
Human ovarian epithelial-serous carcinoma, bladder dome
COV318 07071903 Human Ovary Human ovarian epithelial-serous carcinoma, peritoneal ascitesCOV362 07071910 Human Ovary Human ovarian epithelial-endometrioid carcinomaCOV362.4 07071904 Human Ovary Human ovarian epithelial-endometrioid carcinoma, pleural effusionCOV434 07071909 Human Ovary Human ovarian granulosa tumourCOV504 07071902 Human Ovary Human ovarian epithelial-serous carcinoma, pleural effusionCOV644 07071908 Human Ovary Human ovarian epithelial-mucinous carcinoma, primary tumourAVG-16 06062701 Pig Ovary follicle Porcine Reversibly Immortalised Primary Granulosa Cells22Rv1 05092802 Human Prostate Human Xenograft Prostate
VCaP 06020201 HumanProstate metastasis
Human Prostate Cancer Metastasis
HRA-16 06061905 Human Rectum Human rectal adenocarcinomaRB247C 06070601 Human Retina Human RetinoblastomaWERI 06070602 Human Retina Human RetinoblastomaFTC-236 06030202 Human Thyroid Human Thyroid Cancer Neck Lymph Node MetastasisTEC61 06031505 Human Thyroid Human thyroid, endothelial cell strain
New Serum Free Cell Lines – available from ECACC
Serum/Protein Free Adapted Cell LinesCell Line Cat no. Cell Line Cat no.293 GTP-AC-free 05011003 MDCK-Protein Free 02050101293-Hektor 05030204 NS0-Serum-Free 03061601BHK-InVitrus 05062302 NS0-Turbodoma * 06020202CHO (PROTEIN FREE) 00102307 PNT1A (SERUM FREE)* 07052901CHO/dhFr-AC-free * 05011002 PNT2 (SERUM FREE)* 07042701COS-1-InVitrus 04092902 Sf21 TitreHigh AC free 05030202COS-7-InVitrus * 05063001 Sf9 TitreHigh AC free 05011001HeLa (AC-Free) * 08011102 SP2/0-Ag14 (AC-free)* 08060101L929S 86032004 Sp2/0-Ag14-TurboDoma 03092501L-M 87032401 Vero (AC-free)* 08011101
*New since last edition of ECACC hardcopy catalogue
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Note: Listing ordered by ‘Tissue’ i.e. the tissue the cell lines have been derived from.
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Congratulations to the winners of our Winter 2008 crossword competition: Dr. David Jones, PICR, Manchester • Mr Simone Weidlich, Ninewells Hospital & Medical School, Dundee • Mr Hiro Yoshida, MRC LMB, Cambridge
Across
1 Positively charged amino acid (6)4 Virus infection, causes genital sores (6)6 Canine (3)7 Salt solution (6)8 Postulated how to prove cause
of infectious disease (4)11 Ornamental fi sh (4)13 Extra-chromosomal bacterial DNA, vector
used in genetic engineering (7)14 At the end of a pipette (3)15 Bacterial sexually transmitted disease (9)
W
Remember visit www.hpacultures.org.uk/promotions/crossword.jsp to enter the competition.
Good Luck!
Another chance to win one of three i-pod shuffles in your choice of colour!WIN!WIN!
Down
1 Detoxifying organ (5)2 A cycle, aka citric acid cycle,
energy producing, anagram of breaks without a. (5)
3 Acetic acid, good on chips (6)4 Hates water (11)5 Of a low pH (6)9 Three copies of a chromosome (7)10 Oral fl uid (6)12 String of amino acids (6)
For another chance to win one of three i-Pod Shuffl es in your preferred colour:
Use the letters in the orange squares of your completed crossword to form three fi ve letter words (clue: describes a product or service available from ECACC):
Products and Services available from the HPA Culture Collections:
ll Cell Lines and Hybridomas
ll Primary Cells
ll DNA Products
ll Bacteria, Plasmids, Transposons
ll Mycoplasmas
ll Fungi
ll LENTICULE Discs
ll Viruses
Visit www.hpacultures.org.uk for more information!
ll Cell Culture Management Services
ll Contract Cell Culture
ll Assay Ready Cells
ll Cell Line Identity Verifi cation
ll DNA Extraction
ll Genetic Support Services
ll Contract Freeze-Drying
l Mycoplasma Testing &
Eradication
l NCPV Contract Services
l Patent Deposits
l Safe Deposits
l Sterility Testing
l Training
Products: Services
HPA Culture CollectionsA strategic business unit within the Health Protection Agency.
www.hpacultures.org.uk Tel: +44 (0) 1980 612512 email: [email protected]
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Then visit our website at the crossword entry page www.hpacultures.org.uk/promotions/crossword.jsp and submit your answer. Closing date: 31st May 2009.
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