1. 1. SPRING TERM 2015 AMELIA PAYNES EXIBITION
2. 2. INTRODUCTION Weiss Lab Classes Visualizing Algebra Chemistry Italian Crash Course World History Q & A
3. 3. THIS IS NOT A GATEWAY EXHIBITION
4. 4. WEISS LAB 2015
5. 5. AMAZING PEOPLE Thank you to all the amazing people who gave me this opportunity, and helped me learn
6. 6. PROJECTS HARVESTING MOUSE LUNGS PHOTOGRAPHING MICROSCOPY SLIDES PH TESTING SODIUM DEOXYCHOLATE MYSTERY STORAGE SOLUTIONS
7. 7. HARVESTING MOUSE LUNGS To be used in experiments involving the decellularization and recellularization of lungs Lung Cannula in tracia Lung being ventilated during recelurazation WHY Reacherch involving the removal of cells(decelurazation) and re-growing cells(recellurazation) may eventually help us to grow human lungs for transplant
8. 8. PHOTOGRAPHING SLIDES Of Decellurised and Recellurised lung and assembling PowerPoints of the slides Recellurised mouse lung slide ventilated with 150ml of airThe microscope used to photograph slides Native pig lung slide
9. 9. PH TESTING WHY: Ph. tested washes to insure that the PH would not effect the decal process unduly HOW: a Ph. testing system. Small amounts of each weak acids or bases and added to neutralize the Ph. The small amounts( > 35 l in most cases demonstrated) that the Ph was close to neutral The Ph. was close to neutral and will not impact the decell process
10. 10. DATA Average Ph. Of washes Di Triton NaCl DNase 6.11 6.42 4.9 6.28 DI Triton NaCl Dnase 7.35 7.39 7.35 7.79 DI Triton NaCl Dnase 20.5 29.4 25.4 1.25 Average Ph. ReachedAverage NaOH to neutral UL
11. 11. CHEMISTRY CONNECTION I learned about chemical reactions and writing formulas in chemistry class When doing the PH neutralizing experiment a interesting reaction occurred. Adding the soluble hydrochloric acid to the soluble Sodium deoxycholate solution caused a insoluble participate to form, I set out to find out why The Plot Thickens
12. 12. THEORY'S I suspected a chemical reaction like the ones I had been learning about in chemistry class I wrote a formula like I had and researched the solubility of possible products All the possible outcomes were soluble but a precipitate had formed. Why? The formula HCl(aq) + C24H39NaO4(aq) x(aq) + y(s)
13. 13. LIKE ANYONE EVER GOT A GOOD ANSWER ON YAHOO
14. 14. CASE CLOSED It was my former mentor Darcy who eventually solved the riddle It wasn't a chemical reaction at all Sodium Dexoycolate is soluble in water under a certain set of conditions It is soluble when the PH is above 5 The added hydrochloric acid locally dropped the Ph. below 5 causing the Sodium Dexoycolate to leave solution Product information sheets contain useful data, like solubility conditions. Always check the product information sheet, and the material safety data sheet for any chemical you are using HCl(aq) + C24H39NaO4(aq) HCl(aq) + C24H39NaO4 (s) The formula
15. 15. STORING LUNGS We can not store decellurised lungs for longer then 3 months Enzymes may digest proteins, making the lungs unviable for recellurization Water crystals are sharp and can damage cells and matrix. Water also expands and bursts cells when it freezes. Cryogenic chemicals that lower the freezing point are often dangerous to cells I researched methods used to store skin grafts
16. 16. SOLUTIONS Freezing at a controlled rate in a controlled rate freezer in a cryogenic solution is another In the future I will help to create a protocol for preserving the decellurised lung matrix Deep freezing in liquid nitrogen may be a possible method propylene glycol a cryogenic solution
17. 17. WHAT I LEARNED To work hard How to take clear miscopy photos How tissue is stored How to test for Ph. How to interact with coworkers How to win sparkle quizzes when One person cant spell and the other only knows the answers in germen
18. 18. Q & A