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OIE RL for « Foot and Mouth Disease » – « Dr Jef Hammond » – « United Kingdom »

OIE Reference Laboratory ReportsActivities in 2012

Name of disease (or topic) for which you are a designated OIE Reference Laboratory:

FMD

Address of laboratory The Pirbright Institute

Ash Road, Pirbright, Surrey GU24 0NF

United Kingdom

Tel.: +44 1483 231211

Fax: +44 1483 232621

e-mail address: [email protected]

website: http://www.pirbright.ac.uk

Name (including Title) of Head of Laboratory (Responsible Official):

Director: Professor John K Fazakerley

Name (including Title and Position) of OIE Reference Expert:

Dr Jef Hammond- Head Reference Laboratories for FMD and SVD

Date of submission to the OIE 25-01-13

Instructions

This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.

Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at:http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/

Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.

Please note the red italicised text is given as guidance and should be deleted from your report and substitute with your data. Examples are based on past Annual Reports or have been invented.

The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.

This annual report will remain available for consultation on the OIE web site:(http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):

Annual reports of OIE Reference Centres, 2012 1

http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/

http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/

http://www.pirbright.ac.uk/

mailto:[email protected]


ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards

Test For Specificity Total

Cell culture Virus isolation Group 685

RT-PCR Virus genome detection Group 2872

ELISA Antigen Serotype 900

VP1 sequencing Phylogeny Strain 380

Serology Vaccine Matching ’r’ values 1192

VNT Antibody Serotype 3435

ELISA Structural Protein Antibody Serotype 4383

ELISA Non-structural protein Antibody Non-serotype 1433

ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.

To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.

2. Did your laboratory produce or store imported standard reference reagents officially recognised by the OIE or other international bodies?

Yes

3. Did your laboratory supply standard reference reagents to OIE Member Countries?

Yes

2 Annual reports of OIE Reference Centres, 2012


Type of reagent available

Related diagnostic test

Produced/stored

Supplied nationally

Supplied to EU Member States

Supplied to Other Countries

Name of recipient OIE Member Countries and of institutions

Rabbit and Guinea Pig Antisera

Ag and Ab ELISA Produced None 13ml 141ml Canada NCFAD, Winnipeg

Israel Kimron Veterinary InstituteKenya SAHSP, Nairobi

Iraq Vet. Central Lab, BagdadTunisia Institut Pasteur de Tunis

Turkey SAP Institute, AnkaraVietnam National Centre Vet. Diag., Ha Noi

Uganda NADDEC, Entebe

FMD Reference sera

Ag and Ab ELISA Produced None 24ml 37ml Iraq Vet. Central Lab, Bagdad

Romania Inst. Diagn. Animal HealthSouth Korea Animal Plant & Fish. Q&I Agency, Gyeonggi

Province

Vietnam National Centre Vet. Diag.

Inactivated virus Ag and Ab ELISA Produced None 70ml 87ml Iraq Vet. Central Lab, BagdadKenya SAHSP, Nairobi

Romania Inst. Diagn. Animal HealthTurkey SAP Institute, Ankara

Vietnam National Centre Vet. Diag.

Live virus VNT Produced 38ml 30ml 50ml The Netherlands- Central Veterinary InstituteMerial-UKIntervet/MSD- Germany

Antigen ELISA kits Ag ELISA Produced None 2 20 Bulgaria Bulgarian Food Safety AgencyCroatia Serology Lab. Viral disease, Zagreb

Egypt Animal Health Research Institute, GizaEstonia Estonian Veterinary & Food Lab, TartuEthiopia Nat. Animal Health Diagnostic & Investigation

Centre, SebetaHongKong Agricult. Fish. & Conserv. Dept.,HKSAR

New Zealand Animal Health Laboratory, Upper HuttOman Vet. Research Laboratory, Muscat

South Korea Animal Plant & Fish. Q&I Agency, Gyeonggi



ProvinceUAE Dept. of President’s Affairs, Abu Dhabi

Uganda NADDEC, EntebeJapan Animal Quarantine Service, Kanagawa

Kazakhstan Research Institute for Biol. SafetyMongolia State Central Veterinary Laboratory

Kuwait Central Vet. Lab. & Animal Research

Antibody ELISA kits

Ab ELISA Produced None 3 82 Algeria Inst. National de la Med. Veterinaire

Bangladesh Livestock Research Institute, DhakaBelarus Belarusian State Vet. Centre, Minsk

Bulgaria Bulgarian Food Safety AgencyCroatia Serology Lab. Viral disease, Zagreb

Estonia Estonian Veterinary & Food Lab, TartuIndonesia Veterinary Biological Centre, Surabaya

Iraq Veterinary Central Laboratory, BagdadJapan Animal Quarantine Service, Kanagawa

Kazakhstan Research Institute for Biol. SafetyKenya SAHSP, NairobiKuwait Central Vet. Lab. & Animal Research

Kyrgyzstan State Veterinary Laboratory, BishkekLAO PD Dept. of Livestock & Fisheries, Vientiane

Malaysia Dept. Veterinary Services, PutrajayaMongolia State Central Veterinary Laboratory

Mozambique Regional Animal Health Dept., Kan ThoPakistan Animal Sciences Institute, Islamabad

Russia Federal Centre Animal Health, VladimirRwanda National Veterinary Laboratory, RubiriziSouth Korea Animal Plant & Fish. Q&I Agency, Gyeonggi

ProvinceSudan Institute Endemic Diseases, Khartoum

Turkey SAP Institute, AnkaraVietnam National Centre Vet. Diag.Zambia Central Vet. Res. Inst., Lusaka



4. Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?

No

5. Did your laboratory produce vaccines?

No

6. Did your laboratory supply vaccines to OIE Member Countries?

No

ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases

7. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?

No

8. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?

No

ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries

9. Did your laboratory carry out diagnostic testing for other OIE Member Countries?

Yes

In total the Pirbright Institute reference laboratory for FMD received 764 samples from 27 countries in 2012, 509 were collected in 2012, 230 were collected in 2011, 21 were collected in 2010, 2 were collected in 2009 and 2 were collected in 2008.

Name of OIE Member Country

seeking assistance

Date of collection (dd/mm)

No. samples received for provision of diagnosis and characterisation of FMD

AFGHANISTAN 07-Apr-1107-Apr-1108-Apr-1117-Apr-1109-May-1128-May-1129-May-1129-May-1130-May-1130-May-1130-May-1130-May-1130-May-1130-May-1130-May-1131-May-1131-May-1131-May-1114-Jun-1116-Aug-11

20



BAHRAIN 05-Mar-1205-Mar-1205-Mar-1205-Mar-1205-Mar-1205-Mar-1205-Mar-1205-Mar-1206-Mar-1206-Mar-1213-Mar-1220-Mar-1221-Mar-1221-Mar-1221-Mar-1226-Mar-1205-Mar-1205-Mar-1205-Mar-1228-Mar-1228-Mar-1228-Mar-1228-Mar-1228-Mar-1228-Mar-1228-Mar-1228-Mar-1205-Apr-1208-Apr-1218-Apr-1219-Apr-1219-Apr-1219-Apr-1222-Apr-1224-Apr-1224-Apr-1225-Apr-1225-Apr-1225-Apr-1225-Apr-1225-Apr-1225-Apr-1225-Apr-1225-Apr-1225-Apr-1225-Apr-1225-Apr-1225-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1226-Apr-1229-Apr-1229-Apr-1229-Apr-1229-Apr-1229-Apr-1229-Apr-1229-Apr-12

129



29-Apr-1229-Apr-1229-Apr-1229-Apr-1229-Apr-1230-Apr-1230-Apr-1230-Apr-1230-Apr-1230-Apr-1230-Apr-1230-Apr-1230-Apr-1230-Apr-1230-Apr-1230-Apr-1230-Apr-1230-Apr-1207-May-1207-May-1207-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1208-May-1209-May-1209-May-1209-May-1209-May-1209-May-1209-May-1209-May-1209-May-1209-May-1209-May-1209-May-1209-May-1209-May-1209-May-1209-May-1224-May-12

BHUTAN 26-Jun-1226-Jun-1226-Jun-1226-Jun-1226-Jun-1226-Jun-1226-Jun-1226-Jun-1226-Jun-12

9

BOTSWANA 21

EGYPT 14-Oct-1015-Oct-1015-Oct-1006-Jan-1109-Jan-1113-Feb-1121-Feb-1111-Apr-1121-Apr-1121-Apr-11

49

Annual reports of OIE Reference Laboratories, 2012 7


19-May-1114-Jun-1113-Jul-1108-Feb-1217-Feb-1218-Feb-1227-Feb-1227-Feb-1227-Feb-1228-Feb-1229-Feb-1229-Feb-1229-Feb-1229-Feb-1229-Feb-1201-Mar-1203-Mar-1204-Mar-1208-Mar-1208-Mar-1209-Mar-1228-Feb-1209-Mar-1213-Mar-1213-Mar-1222-Mar-1202-Apr-1203-Apr-1225-Apr-1208-May-1217-May-1219-May-1219-May-1221-May-1222-May-1222-May-1203-Jun-1204-Jul-1205-Aug-12

ERITREA 16-Nov-1117-Nov-1117-Nov-1117-Nov-1127-Nov-1127-Nov-1105-Dec-1105-Dec-1105-Dec-1105-Dec-1105-Dec-1105-Dec-1105-Dec-1105-Dec-1105-Dec-1105-Dec-1105-Dec-1105-Dec-11

18

ETHIOPIA 15-Jul-1115-Jul-1129-Jul-1129-Jul-1129-Jul-1129-Jul-1119-Sep-1119-Sep-1119-Sep-1104-Oct-1104-Oct-1108-Oct-1108-Oct-1108-Oct-1108-Oct-1108-Oct-1108-Oct-1108-Oct-11

51



14-Oct-1114-Oct-1114-Oct-1114-Oct-1114-Oct-1125-Nov-1125-Nov-1125-Nov-1125-Nov-1129-Nov-1129-Nov-1129-Nov-1129-Nov-1129-Nov-1101-Dec-1101-Dec-1101-Dec-1101-Dec-11

14-Jan-201214-Jan-201203-Feb-201209-Feb-201209-Feb-201209-Feb-201217-Feb-201217-Feb-201220-Mar-201220-Mar-201220-May-201220-May-201229-May-201220-Jun-201220-Jun-2012

HONG KONG, SAR OF PRC

28-Mar-1228-Mar-1228-Mar-12

3

IRAN 19-Sep-1108-Oct-1113-Oct-1115-Oct-1129-Oct-1131-Oct-1108-Nov-1109-Nov-1112-Nov-1113-Nov-1118-Nov-1118-Nov-1119-Nov-1120-Nov-1121-Nov-1121-Nov-1123-Nov-1128-Nov-1111-Dec-1125-Dec-1125-Dec-1126-Dec-1127-Dec-1127-Dec-1131-Dec-1101-Jan-1202-Jan-1203-Jan-1203-Jan-1203-Jan-1210-Jan-1212-Jan-1212-Jan-1214-Jan-1217-Jan-1226-Jan-1226-Jan-12

17-Jun-201223-Jun-2012

48



24-Jun-201224-Jun-201226-Jun-201228-Jun-201230-Jun-201230-Jun-201202-Jul-201209-Jul-201214-Jul-2012

ISRAEL20-Mar-1221-Mar-12 2

JAPAN 17-Apr-101

KENYA 17-Jan-1130-Jan-1102-Feb-1115-Feb-1119-Feb-1119-Feb-1101-Mar-1101-Mar-1113-Mar-11

Apr-1106-Jul-1105-Sep-1105-Sep-1118-Sep-1122-Sep-1123-Sep-1123-Sep-1123-Sep-1127-Oct-1127-Oct-11Mar-2011Mar-2011Mar-2011

23

KUWAIT 20-Dec-1120-Dec-1120-Dec-1120-Dec-1120-Dec-1120-Dec-1127-Dec-1127-Dec-1127-Dec-1107-Jan-1209-Feb-1209-Feb-12

12

LIBYA 18-Dec-1120-Dec-1121-Dec-1121-Dec-1121-Dec-1121-Dec-1121-Dec-1121-Dec-1121-Dec-1121-Dec-1121-Dec-1124-Dec-1124-Dec-1125-Dec-1125-Dec-1125-Dec-1125-Dec-1127-Dec-1129-Dec-1130-Dec-1130-Dec-1130-Dec-1101-Jan-1201-Jan-1201-Jan-1201-Jan-1212-Jan-12

125



12-Jan-1217-Jan-1217-Jan-1218-Jan-1218-Jan-1218-Jan-1218-Jan-1218-Jan-1218-Jan-1218-Jan-1218-Jan-1218-Jan-1215-Feb-1215-Feb-1215-Feb-1215-Feb-1215-Feb-1215-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1216-Feb-1217-Feb-1217-Feb-1217-Feb-1217-Feb-1217-Feb-1217-Feb-1218-Feb-1218-Feb-1218-Feb-1218-Feb-1218-Feb-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1219-Apr-1222-Apr-1222-Apr-1223-Apr-1223-Apr-1223-Apr-1202-May-1202-May-1202-May-1202-May-1202-May-12



02-May-1202-May-1202-May-1202-May-1202-May-1202-May-1202-May-1202-May-1202-May-1202-May-1203-May-1203-May-1203-May-1203-May-1203-May-1203-May-1203-May-1203-May-1203-May-1203-May-1203-May-1203-May-1203-May-12

MALAYSIA 31-Jan-1231-Jan-1231-Jan-1205-Feb-1221-Feb-1228-Mar-1202-Apr-1203-Apr-1203-Apr-1212-Apr-1213-Apr-1213-Apr-1217-Apr-1217-Apr-1218-Apr-1223-Apr-1228-Apr-1230-Apr-12

18

PAKISTAN 23-Dec-1123-Dec-1108-Jan-1208-Jan-1208-Jan-1214-Jan-1214-Jan-1216-Jan-1216-Jan-1216-Jan-1216-Jan-1207-Feb-1209-Feb-1221-Feb-1224-Feb-1220-Mar-1220-Mar-1206-Apr-1207-Apr-1208-Apr-1208-Apr-1211-Apr-1212-Apr-1226-Apr-1227-Apr-1227-Apr-1227-Apr-1227-Apr-1208-May-1209-May-1214-May-1214-May-1230-May-1220-Jun-12

43



20-Jun-1221-Jun-1221-Jun-1230-Jun-1230-Jun-1228-Sep-1205-Nov-1223-Nov-1223-Nov-12

PALESTINIAN AUTONOMOUS TERRITORIES

16-Apr-1216-Apr-12

2

KINGDOM OF SAUDI ARABIA

02-Jul-201207-Jul-201207-Jul-201207-Jul-2012

4

SRI LANKA 09-Sep-0913-Jan-1013-Jan-1018-Jan-1015-Feb-1031-Mar-1031-Mar-1021-Apr-1021-Apr-1002-Jun-1004-Jun-1009-Jun-1002-Dec-1003-Jun-1114-Mar-1215-Jun-12

16

SUDAN 13-Dec-200911-Jan-201012-Jan-201009-Feb-201009-Feb-201009-Feb-201028-Feb-201128-Feb-201128-Feb-201101-Mar-201101-Mar-201101-Mar-201101-Mar-201103-Mar-201103-Mar-201103-Mar-201103-Mar-201103-Mar-201103-Mar-201114-Mar-201114-Mar-201102-May-201102-May-201102-May-201124-Nov-2011

25

TAIWAN, PROVINCE OF CHINA

30-Jan-121

TANZANIA 20-Aug-1120-Aug-1127-Nov-1127-Nov-1127-Nov-1108-Feb-1108-Feb-1103-Feb-1203-Feb-1204-Feb-1204-Feb-1229-Feb-1229-Feb-1201-Mar-1201-Mar-12

59



02-Mar-1202-Mar-1225-Mar-1225-Mar-1217-Apr-1217-Apr-1218-Apr-1218-Apr-1218-Apr-1228-Apr-1228-Apr-1229-Apr-1229-Apr-1230-Apr-1230-Apr-1201-May-1201-May-1202-May-1202-May-1203-May-1203-May-1204-May-1204-May-1205-May-1205-May-1225-May-1227-May-1227-May-1227-May-1231-May-1231-May-1201-Jun-1201-Jun-1202-Jun-1202-Jun-1212-Jul-1212-Jul-1212-Jul-12

THAILAND

29-Aug-1121-Oct-1124-Oct-1124-Oct-1124-Oct-1124-Oct-1124-Oct-1112-Nov-1112-Nov-1123-Nov-1123-Nov-1114-Dec-1118-Jan-1218-Jan-1226-Mar-1226-Mar-1226-Mar-1230-Apr-1202-May-1204-May-1208-May-1218-May-1218-Jan-1230-Apr-12

24

TURKEY 20-Oct-1117-Nov-1124-Nov-1101-Dec-1106-Dec-1107-Dec-1108-Dec-1112-Dec-1116-Dec-1119-Dec-1127-Dec-1127-Dec-1127-Dec-11

20



29-Dec-1129-Dec-1102-Jan-1205-Jan-1206-Jan-1210-Jan-1211-Jan-12

UNITED ARAB EMIRATES

23-Dec-111

VIETNAM, SOCIALIST REPUBLIC OF

04-Feb-0818-Jul-0822-Feb-1110-Mar-1121-Mar-1128-Sep-1128-Sep-1101-Dec-1101-Dec-1105-Jan-1216-Jan-1203-Feb-1204-Feb-1206-Feb-1222-Feb-1214-Mar-1214-Mar-1214-Mar-1214-Mar-1214-Mar-1224-Mar-1224-Mar-1216-Apr-1216-Oct-1216-Oct-1216-Oct-1216-Oct-1216-Oct-1216-Oct-1205-Nov-1205-Nov-12

31

ZAMBIA 9

Additional Serotyping data for samples received to Pirbright Reference Laboratory for FMD 2012

Country No. Virus isolation in cell culture/ELISART-PCR for FMD (or

SVD)

of FMD virus serotypes SVD NVD NTvirus (where appropriate)

samples O A CSAT

1SAT

2SAT

3Asia

1 virus Positive Negative NT

AFGHANISTAN 20 3 4 - - - - 5 - 8 - 20 - -

BAHRAIN 129 2 - - - 5 - - - 73 53 9 109 11

BHUTAN 9 - - - - - - - - 9 - - 9 -

BOTSWANA 21 - - - - 21 - - - - - 21 - -

EGYPT 49 7 11 - - 18 - - - 13 - 45 3 1

ERITREA 18 15 - - - - - - - 3 - 16 2 -

ETHIOPIA 51 19 - - - - - - - 32 - 43 7 1



HONG KONG, SAR OF PRC 3 1 - - - - - - - 2 - 3 - -

IRAN 48 9 15 - - - - 20 - 4 - 45 3 -

ISRAEL 2 2 - - - - - - - - - 2 - -

JAPAN 1 1 - - - - - - - - - 1 - -

KENYA a 23 9 - - 1 10 - - - 6 - 18 5 -

KUWAIT 12 12 - - - - - - - - - 12 - -

LIBYA 125 15 - - - 3 - - - 107 - 47 78 -

MALAYSIA 18 6 3 - - - - - - 9 - 17 1 -

PAKISTAN 43 - 3 - - - - 7 - - - 10 - -PALESTINIAN AUTONOMOUS TERRITORIES 2 - - - - 1 - - - 1 - 2 - -

SAUDI ARABIA 4 4 - - - - - - - - - 4 - -

SRI LANKA 16 6 - - - - - - - 10 - 11 5 -

SUDAN 25 6 4 - - 1 - - - 14 - 22 3 -TAIWAN, PROVINCE OF CHINA 1 1 - - - - - - - - - 1 - -

TANZANIA 59 2 2 - 8 23 - - - 24 - 48 11 -

THAILAND 24 4 19 - - - - - - 1 - 24 - -

TURKEY 20 1 6 - - - - 12 - 1 - 20 - -

UNITED ARAB EMIRATES 1 1 - - - - - - - - - 1 - -VIETNAM, SOCIALIST REPUBLIC OF 31 20 6 - - - - - - 5 - 31 - -

ZAMBIA 9 - - - 4 2 - - - 3 - 9 - -TOTAL 764 146 73 - 13 84 - 44 - 325 53 482 236 13

VI/ELISA FMD (or SVD) virus serotype identified following virus isolation in cell culture and antigen detection ELISA

FMD foot-and-mouth disease

SVD swine vesicular diseaseNVD no FMD, SVD or vesicular stomatitis virus detected

RT-PCR reverse transcription polymerase chain reaction for FMD (or SVD) viral genome

NT not tested

a 3 samples from Kenya contained a mixture of FMDVs of types O and SAT2



10. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?

Yes

Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was

provided

Dr Soheir Hassan Abd El Kader Under Secretary of centeral adminstration of preventive medicineGeneral Organization for Veterinary ServicesMinistry of AgricultureCairo,

EGYPT

Disease Control Advice, vaccine choice advice during the outbreaks of FMD in North Africa first half of 2012

Results of laboratory tests, serotyping, sequencing and vaccine matching.Telephone, fax and email

Attended workshop in Cairo to present WRLFMD® results and advice

Numerous countries receive ad hoc advice on a weekly basis

FMD reporting queries, vaccine matching advice and disease control advice

In person at meetings, telephone, email and fax

ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations

11. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?

Yes

Title of the study Duration Purpose of the study Partners (Institutions)

OIE Member Countries involved other than

your country

Combating Infectious Diseases in Livestock

for International Development

(CIDLID)

3 years to improve FMD control in East and Southern Africa.

BBSRC/DfID/ Scottish Executive and the

Welcome Trust (Southern African

Centre for Infectious Disease Surveillance)

TanzaniaIndia

RAPIDIA-FIELD (http://rapidia.eu/)

develop and evaluate new diagnostic tools that can be

used in the field and in simple laboratories;

EU collaborative research projects:

EU member States

Epi-Seq develop new approaches for molecular epidemiology

using next-generation sequencing platforms.

EU collaborative research projects:

EU member States

ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases

12. Did your Laboratory collect epizootiological data relevant to international disease control?

Yes

13. Did your laboratory disseminate epizootiological data that had been processed and analysed?

Yes

14. What method of dissemination of information is most often used by your laboratory?(Indicate in the appropriate box the number by category)

a) Articles published in peer-reviewed journals:................. 30


https://owa.iah.ac.uk/owa/redir.aspx?C=o9xsMY89k06fdDk5UVtgm7FNoiw1088IqRMKwFzIcs5l7oBKVxWWTn2M6i88XC2QbKYmVTOeDeI.&URL=http%3A%2F%2Frapidia.eu%2F


b) International conferences:.............................................. 50+

c) National conferences:...................................................... 20

d) Other:.............................................................................. internet website, disease reports, annual, quarterly reports on global disease status

*Publications list attached below

ToR: To provide scientific and technical training for personnel from OIE Member Countries

To recommend the prescribed and alternative tests or vaccines as OIE Standards15. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE

Member Countries?

Yes

If the answer is yes, please provide the total number of trained persons for each of the following categories: This section is difficult for me to interpret

WRLFMD carries out extensive training throughout the year to a number of countries including technical and strategic visits, presentations, our 2 week training course and internships. Specific details for some activities are below.

a) Technical visits:................................................................ 10

b) Seminars:.........................................................................50

c) Hands-on training courses:.............................................. 2

d) Internships (>1 month):...................................................6

Type of technical training provided (a, b, c or d)

Country of origin of the expert(s) provided with training

No. participants from the corresponding country

d Dr Christopher Kasanga from Sokoine University of Agriculture Tanzania visited the Pirbright Institute for 2 months (November-December 2012) to undertake training in FMDV sequencing.

1

c the annual 2-week FMDV diagnostics training course was held at Pirbright for overseas scientists between 30/04/12-11/05/12

Diagnostic Course Attendees:Adriana leite : BrazilRichard Clough : New ZealandRuben Villalba Martinez : Spain Robert Mwebe : UgandaMartin Esau : UgandaJessica Dawson : ZimbabweMoses Dhikusooka : Uganda

Diagnostic Course Attendees:1: Brazil1: New Zealand1: Spain3: Uganda1: Zimbabwe

a Dr Yanmin Li provided a week on site training and consultancy programme on serology diagnostic technologies in The National Veterinary Laboratory, Gaborone, Botswana 19th-23rd November 2012

20:The National Veterinary Laboratory, Gaborone, Botswana

a Raphael Sallu from the visited the group on the 1 : Central Veterinary Laboratory,



1st February Dar-es-salaam, Tanzania

a/c Dr Tiziana Lembo from the is visiting the group to participate in testing of samples collected in Tanzania (as part of the UoG BBSRC CIDLID project)

1 : Tanzania/University of Glasgow

a Dr Guido Konig from visited the group on 26th February

1 : INTA, Argentina

ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned

16. Does your laboratory have a Quality Management System certified according to an International Standard?

Yes

Quality management system adopted

ISO 17025

17. Is your laboratory accredited by an international accreditation body?

Yes

Test for which your laboratory is accredited Accreditation body

WRL 002 Processing field samples for diagnosis and growth of vesicular diseases WRL 006 FMDV and SVDV antigen detection by ELISA WRL 033 Svanova 1F10 lateral flow device for FMDV antigen detection WRL 026* One step TaqMan® RT-PCR for diagnosis of FMDV and related vesicular

diseases (*plus supporting documents) SAU 004 Detection of antibodies against vesicular and related viruses by the virus

neutralisation test (VNT) SAU 005 Liquid Phase Blocking ELISA (LPBE) for detection of antibodies against

Foot-and-Mouth disease virus (FMDV) SAU 010 Detection of Antibodies against the Non Structural Protein of Foot-and-

Mouth disease virus (FMDV) using Ceditest® FMDV-NS (PrioCHECK® FMDV –NS) kits

SAU 011 Detection of Antibodies against the Structural Protein of Foot-and-Mouth disease virus (FMDV) by solid-phase competition ELISA (SPCE)

SAU 012 Detection of Antibodies against Foot and Mouth disease virus (FMDV) using PrioCHECK® FMDV type O kits

SVD 008 5B7-Monoclonal Antibody Competition (MAC) ELISA for the detection of antibodies against SVD virus

UKAS

18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)

Yes

ToR: To organise and participate in scientific meetings on behalf of the OIE19. Did your laboratory organise scientific meetings on behalf of the OIE?

Yes

National/International

Title of event Co-organiser Date (mm/yy)

Location No. Participants



International hosted, chaired, presented and organised with Dr Yanmin Li OIE/FAO FMD reference laboratories network meeting

OIE and FAO 1st-2nd

NovemberJerez, Spain. 35

20. Did your laboratory participate in scientific meetings on behalf of the OIE? Representatives from the FMD reference laboratory visit many countries and attend many meetings and always they proudly represent the OIE interests at such meetings. See attached publications and presentations list.

Yes

Title of event Date (mm/yy)

Location Role (speaker, presenting poster, short communications)

Title of the work presented

SEACFMD 2nd – 5th

MarchYunnan, P.R.

ChinaSpeaker FMD Global Situation

ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results

21. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?

Yes

22. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?

Yes

Purpose of the proficiency tests: (validation of a diagnostic protocol: specify the test; quality control of

vaccines: specify the vaccine type, etc.)

Role of your Reference Laboratory (organiser/

participant)No.

participants

Participating OIE Ref. Labs/

organising OIE Ref. Lab.

To complete a proficiency testing study for virology and serology diagnosis for FMD and

SVD during 2012.

Organiser 59 countries For details see Q.24

23. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?

Yes

Title of the project or contact Scope Name(s) of relevant OIE Reference Laboratories



Continued collaboration with ISZLER, Brescia, Italy,

Developing new FMD diagnostic kits for Antigen and antibody detection and validation of the SPCE kit for FMDV type O, A and Asia 1.

ISZLER, Brescia, Italy,WRLFMD at the Pirbright Institute.

Validation of Diagnostic assay for vesicular diseases:

Development of in-house FMDV Asia I strip test and SVD empty capsid cELISA”

The NCFAD, Canadian Food Inspection Agency and the WRLFMD at the Pirbright Institute.

SADC TADs Surveillance campaign of buffalo from Malawi, Tanzania and Zambia

OIE RL for FMD, South Africa and OIE RL for FMD, BotswanaWRLFMD at the Pirbright Institute.

EPIZONE Develop a full genome sequence approach within OIE RLs

OIE RL for FMD, Russia and WRLFMD at the Pirbright Institute.

ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results.

24. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?

Yes

Purpose for inter-laboratory test comparisons1 No. participating laboratories

Participating OIE Member Countries

To complete a proficiency testing study for virology and serology diagnosis for FMD and SVD during 2012. Particular tests were not specified, but labs were invited to select tests and interpret results based upon:

a. Virus detection – as if samples were from suspected FMD/SVD outbreak cases.

b. Serology - as if these serum samples were from FMD/SVD suspected cases with possible O1 Manisa or Asia 1 Shamir vaccination history.

59

Austria, Belgium, Bulgaria, CyprusCzech Republic, Denmark, Estonia, Finland, France, Germany, GreeceHungary, Ireland, Italy, Latvia, Lithuania, Malta, Netherlands, Poland, Portugal, Romania, Slovak Republic, Slovenia, Spain, Sweden, UK AHVLA – Lynne Hendry(FMD)/Sonjia Kelly(SVD)UK IAH Pirbright, Total (labs/countries)Algeria, Armenia, AzerbaijanBelarus, Bosnia (Velic), Bosnia (Maric)Botswana, Brazil, China(3 labs), CroatiaEgypt, Georgia, India, Iran, IsraelMacedonia, Moldova, Morocco (Zro)Morocco (Harrak), Norway, Russia, Serbia, South Africa, SwitzerlandSyria, Tunisia, Turkey, UkraineTotal (labs/countries)Albania, Argentina, AustraliaCanada, Chile, Ethiopia, Hong Kong, Iraq, Ivory Coast, Japan, Kenya, KosovoLebanon, Libya, South Korea, NigeriaPakistan, Thailand, Taiwan, U A EUganda, U S A-

ToR: To place expert consultants at the disposal of the OIE25. Did your laboratory place expert consultants at the disposal of the OIE?

Yes

1 See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3


http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing%20see%20point%201.3


Kind of consultancy Location Subject (facultative )

ad hoc Group Paris FMD country Freedom

Provision of comments on OIE Standards Pirbright Comments to FMD Chapter OIE Terrestrial Manual

Chair of the OIE/FAO FMD Reference Laboratory Network

Pirbright To Chair, coordinate, manage workplan and produce annual report

Publications and Presentations 2012

Attendance at International Meetings

Dr Jef Hammond - Head OIE Reference Laboratory for FMD.

25th-27th January - EFSA SVD meeting Parma Italy 16th February SCAD OIE Paris presentation entitled ‘FMD Global update and network activities’. 28th February-2nd March Bangkok International Congress of Virology; 2 presentations entitled ‘ FMD :

Infectivity, Transmission and Pathogenicity’ ICVS, Bangkok, March 1st 2012and ‘ FMD Global Situation- Vaccine Recommendations and Prospects for Improved Vaccines’ ICVS 1st March 2012.

2nd – 5th March-congress and SEACFMD; presentation entitled ‘FMD Global Situation’ 26th -29th March EuFMD Istanbul WestEurasia; 3 presentations entitled ‘FMD Global update and network

activities’, ‘FMD Vaccine Selection’ and ‘The Combined FMD and SVD Proficiency Test Scheme (PTS) Exercise 2011’

11th – 14th April- 83rd Executive Committee of the EuFMD, Bucharest, Hungary. 20th April- Prionics Geneva, Switzerland. 19th-22nd May- OIE General Session Paris, France. 25th- 29th June -FAO/OIE Global Conference on Foot and Mouth Disease Control, Bangkok, Thailand. 20th – 24th August Attended Australian Industry forum on FMD, Sydney Australia. 4th-5th October hosted EuFMD Executive meeting at Pirbright. Presented several papers. 13th-19th October attended meeting at Lanzhou China discussions on closer partnerships and collaboration

with Chinese FMD research and diagnostics team. 28th 31st October EuFMD meeting in Jerez, Spain. Gave presentation on global FMD incidence and chaired

an evening session. 1st-2nd November hosted, chaired, presented and organised with Dr Yanmin Li OIE/FAO FMD reference

laboratories network meeting in Jerez, Spain. 12th-15th November visited USDA in Baltimore, USA hosted by the US CVO Dr John Clifford and his

team to present current FMD situation, control strategies and vaccine bank information. 27th November teleconference with International FMD strategic reserves network (FMD vaccine bank

network)

Published Reports

OIE/FAO Quarterly reports for 2012 were produced and widely circulated1. January-March2. April-June3. July-September4. October- December

The 2011 annual report of the OIE/FAO FMD Reference Laboratory Network was compiled, edited and distributed by Dr Jef Hammond.

Publications

1. Knowles N. J., He J., Shang Y., Wadsworth J., Valdazo-González B., Onosato H., Fukai K., Morioka K., Yoshida K., Cho I.-S., Kim S.-M., Park J.-H., Lee K.-N., Luk G., Borisov V., Scherbakov A., Timina A., Bold D., Nguyen T., Paton D. J., Hammond J. M., Liu X. and King D. P. Emergence of



Southeast Asian Foot-and-mouth disease viruses in East Asia. Emerging Infectious Diseases. (IN PRESS) DOI: 10.3201/eid1803.110908. Expedited for publication (article featured on website)

2. Mahapatra M, Hamblin P, Paton DJ. Foot-and-mouth disease virus epitope dominance in the antibody response of vaccinated animals. J Gen Virol. 2012 Mar;93(Pt 3):488-93. Epub 2011 Dec 7. PubMed PMID: 22158876.

3. Ferris NP, Clavijo A, Yang M, Velazquez-Salinas L, Nordengrahn A, Hutchings GH, Kristersson T, Merza M. Development and laboratory evaluation of two lateral flow devices for the detection of vesicular stomatitis virus in clinical samples. J Virol Methods. 2012 Mar;180(1-2):96-100. Epub 2011 Dec 29. PubMed PMID: 22230813.

4. Ko YJ, Lee HS, Park JH, Lee KN, Kim SM, Cho IS, Joo HD, Paik SG, Paton DJ, Parida S. Field application of a recombinant protein-based ELISA during the 2010 outbreak of foot-and-mouth disease type A in South Korea. J Virol Methods. 2012 Jan;179(1):265-8. Epub 2011 Oct 5. PubMed PMID: 22001272.

5. Howey R, Bankowski B, Juleff N, Savill NJ, Gibson D, Fazakerley J, Charleston B, Woolhouse ME. Modelling the within-host dynamics of the foot-and-mouth disease virus in cattle. Epidemics. 2012 Jun;4(2):93-103. Epub 2012 Apr 26. PubMed PMID: 22664068.

6. Fowler V, Robinson L, Bankowski B, Cox S, Parida S, Lawlor C, Gibson D, O'Brien F, Ellefsen B, Hannaman D, Takamatsu HH, Barnett PV. A DNA vaccination regime including protein boost and electroporation protects cattle against foot-and-mouth disease. Antiviral Res. 2012 Apr;94(1):25-34. Epub 2012 Feb 11. PubMed PMID: 22330893.

7. Seago J, Jackson T, Doel C, Fry E, Stuart D, Harmsen MM, Charleston B, Juleff, N. Characterization of epitope-tagged foot-and-mouth disease virus. J Gen Virol. 2012 Nov;93(Pt 11):2371-81. doi: 10.1099/vir.0.043521-0. Epub 2012 Jul 18. PubMed PMID: 22815275.

8. Juleff N, Valdazo-González B, Wadsworth J, Wright CF, Charleston B, Paton DJ, King DP, Knowles NJ. Accumulation of Nucleotide Substitutions Occurring During Experimental Transmission of Foot-and-Mouth Disease Virus. J Gen Virol. 2012 Oct 3. [Epub ahead of print] PubMed PMID: 23034594.

9. Carr BV, Lefevre EA, Windsor MA, Inghese C, Gubbins S, Prentice H, Juleff ND, Charleston B. CD4+ T-Cell Responses to Foot-and-Mouth Disease Virus in Vaccinated Cattle. J Gen Virol. 2012 Oct 3. [Epub ahead of print] PubMed PMID: 23034593.

10. Ahmed HA, Salem SA, Habashi AR, Arafa AA, Aggour MG, Salem GH, Gaber AS, Selem O, Abdelkader SH, Knowles NJ, Madi M, Valdazo-González B, Wadsworth J, Hutchings GH, Mioulet V, Hammond JM, King DP. Emergence of Foot-and-Mouth Disease Virus SAT 2 in Egypt During 2012. Transbound Emerg Dis. 2012 Oct 1. doi: 10.1111/tbed.12015. [Epub ahead of print] PubMed PMID: 23025522.

11. Berryman S, Brooks E, Burman A, Hawes P, Roberts R, Netherton C, Monaghan P, Whelband M, Cottam E, Elazar Z, Jackson T, Wileman T. FMDV induces autophagosomes during cell entry via a class III PI3K-independent pathway. J Virol. 2012 Sep 19.[Epub ahead of print] PubMed PMID: 22993157.

12. Madhanmohan M, Nagendrakumar SB, Manikumar K, Yuvaraj S, Parida S, Srinivasan VA. Development and evaluation of a real-time reverse transcription-loop-mediated isothermal amplification assay for rapid serotyping of foot-and-mouth disease virus. J Virol Methods. 2012 Aug 30. pii: S0166-0934(12)00294-7. doi:10.1016/j.jviromet.2012.08.015. [Epub ahead of print] PubMed PMID: 22960423.

13. Valdazo-González B, Knowles NJ, Hammond J, King DP. Genome sequences of SAT 2 foot-and-mouth disease viruses from Egypt and Palestinian Autonomous Territories (Gaza Strip). J Virol. 2012 Aug;86(16):8901-2. PubMed PMID: 22843860; PubMed Central PMCID: PMC3421706.



14. Li Y, Swabey KG, Gibson D, Keel PJ, Hamblin P, Wilsden G, Corteyn M, Ferris NP. Evaluation of the solid phase competition ELISA for detecting antibodies against the six foot-and-mouth disease virus non-O serotypes. J Virol Methods. 2012 Aug;183(2):125-31. Epub 2012 Apr 25. PubMed PMID: 22561986.

15. Juleff ND, Maree FF, Waters R, Bengis RG, Charleston B. The importance of FMDV localisation in lymphoid tissue. Vet Immunol Immunopathol. 2012 Jul 15;148(1-2):145-8. Epub 2011 May 7. PubMed PMID: 21616546.

16. Madi M, Hamilton A, Squirrell D, Mioulet V, Evans P, Lee M, King DP. Rapid detection of foot-and-mouth disease virus using a field-portable nucleic acid extraction and real-time PCR amplification platform. Vet J. 2012 Jul;193(1):67-72. Epub 2011 Nov 23. PubMed PMID: 22115952.

17. Berryman S, Brooks E, Burman A, Hawes P, Roberts R, Netherton C, Monaghan P, Whelband M, Cottam E, Elazar Z, Jackson T, Wileman T. Foot-and-mouth disease virus induces autophagosomes during cell entry via a class III phosphatidylinositol 3-kinase-independent pathway. J Virol. 2012 Dec;86(23):12940-53. doi: 10.1128/JVI.00846-12. Epub 2012 Sep 19. PubMed PMID: 22993157; PubMed Central PMCID: PMC3497631.

18. Ahmed HA, Salem SA, Habashi AR, Arafa AA, Aggour MG, Salem GH, Gaber AS, Selem O, Abdelkader SH, Knowles NJ, Madi M, Valdazo-González B, Wadsworth J, Hutchings GH, Mioulet V, Hammond JM, King DP. Emergence of foot-and-mouth disease virus SAT 2 in Egypt during 2012. Transbound Emerg Dis. 2012 Dec;59(6):476-81.doi: 10.1111/tbed.12015. Epub 2012 Oct 1. PubMed PMID: 23025522.

19. Porta C, Xu X, Loureiro S, Paramasivam S, Ren J, Al-Khalil T, Burman A, Jackson T, Belsham GJ, Curry S, Lomonossoff GP, Parida S, Paton D, Li Y, Wilsden G, Ferris N, Owens R, Kotecha A, Fry E, Stuart DI, Charleston B, Jones IM. Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity. J Virol Methods. 2012 Nov 19. doi:pii: S0166-0934(12)00391-6. 10.1016/j.jviromet.2012.11.011. [Epub ahead of print] PubMed PMID: 23174161.

20. Seago J, Jackson T, Doel C, Fry E, Stuart D, Harmsen MM, Charleston B, Juleff N. Characterization of epitope-tagged foot-and-mouth disease virus. J Gen Virol. 2012 Nov;93(Pt 11):2371-81. doi: 10.1099/vir.0.043521-0. Epub 2012 Jul 18. PubMed PMID: 22815275.

21. Morelli MJ, Thébaud G, Chadœuf J, King DP, Haydon DT, Soubeyrand S. A bayesian inference framework to reconstruct transmission trees using epidemiological and genetic data. PLoS Comput Biol. 2012 Nov;8(11):e1002768. doi:10.1371/journal.pcbi.1002768. Epub 2012 Nov 15. PubMed PMID: 23166481; PubMed Central PMCID: PMC3499255

22. Kasanga C. J., Sallu R., Kivaria F., Mkama M., Masambu J., Yongolo M., Das S., Mpelumbe-Ngeleja C., Wambura P. N., King D. P., and Rweyemamu M. M. (2012). Foot-and-mouth disease virus serotypes detected in Tanzania from 2003-2010: conjectured status and future prospects. Onderstepoort Journal of Veterinary Research. 79 (2): 80-83.

23. King D. P., Madi M., Mioulet V., Wadsworth J., Wright C. F., Valdazo-González B., Ferris N. P., Knowles N. J. and Hammond J. (2012) New technologies to diagnose and monitor infectious diseases of livestock: challenges for sub-Saharan Africa. Onderstepoort Journal of Veterinary Research. 79 (2): 43-48.

24. Valdazo-González B., Polihronova L., Alexandrov T., Normann P., Knowles N. J., Hammond J. M., Georgiev G. K., Özyörük F., Sumption K. J. Belsham G. J. and King D. P. (2012) Reconstruction of the transmission history of RNA virus outbreaks using full genome sequences: foot-and-mouth disease virus in Bulgaria in 2011. PLoS ONE 7 (11) e49650.

25. Bhatta D., Marti Villalba M., Johnson C. L., Emmerson G. D., Ferris N. P., King D. P. and Lowe C. R. (2012). Rapid detection of foot-and-mouth disease virus with optical microchip sensors. Procedia Chemistry 6: 2-10.



26. Juleff N., Valdazo-González B., Wadsworth J., Wright C. F., Charleston B., Paton D. J., King D. P. and Knowles N. J. (2013) Accumulation of nucleotide substitutions occurring during experimental transmission of foot-and-mouth disease virus. Journal of General Virology 94: 108-119.

27. Paton D. J. and King D. P. (2013) Diagnosis of Foot-and-mouth disease. Developments in Biologicals (Basel) 135: 117-123.

28. Xu L., Hurtle W., Rowland J. M., Casteran K. A., Bucko S. M., Grau F. R., Valdazo-González B., Knowles N. J., King D. P., Beckham T. R. and McIntosh M. T. Development of a universal RT-PCR for amplifying and sequencing the leader a capsid coding region of foot-and-mouth disease virus. Journal of Virological Methods (IN PRESS).

29. Morelli M. J., Wright C. F., Knowles N. J., Juleff N., Paton D. J. King D. P. and Haydon D. T. Evolution of foot-and-mouth disease virus samples sequence diversity during serial transmission in bovine hosts. Veterinary Research (IN PRESS).

30. Orton R. J., Wright C. F., Morelli M. J., Juleff N. Thébaud G., Knowles N. J., Valdazo-González B., Paton D. J., King D. P. and Haydon D. T. Observing micro-evolutionary processes of viral populations at multiple scales. Philosophical Transactions of the Royal Society B (IN PRESS).


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