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Utilization of waste bread for
lactic acid fermentation
Joachim Venus
Leibniz-Institut für Agrartechnik Potsdam-Bornim e.V. Max-Eyth-Allee 100, D-14469 Potsdam Fon: 0331/5699-112, E-mail: [email protected]
BIOREFINERY FOR THE PRODUCTION OF ENERGY AND
BIO-BASED PRODUCTS
Conference Centre Torino Incontra, Torino, Italy
29-30 October, 2013
1927 Experimental farm of the Agricultural University Berlin
1933 Independent research center on agricultural mechanization
1952 Central institute of agricultural engineering of East Germany
1992 Reestablished after the reunification of Germany
Today: Leibniz Institute for Agricultural Engineering Potsdam-Bornim
- member of the Leibniz Association
History
Technology
assessment in
agricultural
systems
Technologies and processes for crop
production and livestock management
Research structure
08.11.2013 4
Biomass conversion into high-value chemical products and fuels
Biorefineries in theory would use multiple forms of biomass to produce a flexible mix of
products, including fuels, power, heat, chemicals and materials. In a biorefinery, biomass would be
converted into high-value chemical products and fuels (both gas and liquid). Byproducts and
residues, as well as some portion of the fuels produced, would be used to fuel on-site power
generation or cogeneration facilities producing heat and power.
Biotechnological
Venus, J.: Feedstocks and (Bio)Technologies for Biorefineries. – In: G.E. Zaikov, F. Pudel, G. Spychalski (Eds.),
Renewable Resources and Biotechnology for Material Applications (pp. 299-309), Nova Science Publishers, 2011
(ISBN: 978-1-61209-521-9)
08.11.2013 5
Starchy materials (cereals, industrial grade corn/potatoe starch, tapioca)
Green biomass (alfalfa, grass juice, lupine, sweet sorghum, forage rye, silage, coco juice)
Lignocellulosics (wood/straw hydrolysates, 2ndG sugars)
Residues & By-products (bagasse, oilseed cake/meal, thick juice, molasses, whey, coffee residues, waste bread,
waffle residues, algae biomass, fruit residues, meat & bone meal…)
tapioca
bagasse
waste bread
pine
coco juice
2G sugars 2G sugars
1G/2G sugars
green biomass
several residues…
lupine
cereals,
straw
sorghum
Fermentation feedstocks already tested:
silage
algae
biomass
Coffee
residues
08.11.2013 6
Table 1: Overview of chemicals that are currently
produced, or could be produced, from biomass
together with their respective market type, size of
the market, and potential biomass feedstock.
Major players involved are also given.
M.A. Abdel-
Rahman et al.
Journal of
Biotechnology
156 (2011) 286–
301
08.11.2013 7 http://www.bread4pla-life.eu
BREAD4PLA is a LIFE demonstrative European project.
It will demonstrate to the European countries the feasibility of an innovative, user
friendly and sustainable environmental solution which promotes the waste recovery in
the specific agro-food sector of the bakery industry
08.11.2013 8
htt
p:/
/ww
w.b
read4pla
-life.e
u
Example food waste: Bakery industry
1.Bakery waste raw materials
Collection & Characterization
3.Obtaining Polylactic Acid (PLA)
4.Material Development:
Compounding, Film
extrusion (blown film/cast)
5.Characterization of
the material developed
6. Industrial Validation:
Bakery packaging
7: INTERNATIONAL COMMUNICATION AND DISSEMINATION & EXPLOITATION MANAGEMENT
8: PROJECT MANAGEMENT.
2.Obtaining Lactic Acid (LA)
2.Obtaining Lactic Acid (LA)
9
Steps of the demonstration process
BIOSTAT®Bplus (Sartorius BBI Systems GmbH, Germany) equipped with a digital control unit DCU
This European Project is funded by LIFE+ Programme, under grant agreement LIFE 10 ENV ES 479. This ppt reflects the consortium´s opinion and the European Community is not liable for the use of the information contained herein.
ATB´s Activities in the project ACTION 2: OPTIMIZATION OF LACTIC ACID PRODUCTION FROM SELECTED WASTE BY FERMENTATION PROCESS AT PILOT PLANT LEVEL. [Demonstration action]
2.1. Up-stream processing (pre-treatment & hydrolysis of biogenic raw material/waste) 2.2. Lactic acid fermentation
2.3. Down-stream processing (separation & purification of products)
10
0
10
20
30
40
50
60
70
80
90
100
PCP-01 PBV-02 PCT-01 PCT-01plus
SF 1569 SF 1575 SF 1652 SF 1653
[g/L]
Lactate 24hLactate 48hStarch 48hZucker 48h
ACTION 2.2. Comparison of waste bread types
ACTION 2.2. Progress (in bench- and pilot-scale fermentation)
Project Meeting
Bangor (UK) 2013-07-03 11
Fermentation equipment for the scale-up of the process up to 55 (left) and 600 litres (right) of broth liquid, respectively
ACTION 2.2. Progress (in bench-scale fermentation)
12
PCB-01 (crust), enzymatic pre-hydrolysis
0
10
20
30
40
50
60
70
80
90
100
110
0 10 20 30 40 50 60 70
Lacta
te [
g/L]
time [hours]
1 L
55 L
0
5
10
15
20
25
30
0 10 20 30 40 50 60 70Sugars
[g/L]
time [hours]
1 L
55 L
This European Project is funded by LIFE+ Programme, under grant agreement LIFE 10 ENV ES 479. This ppt reflects the consortium´s opinion and the European Community is not liable for the use of the information contained herein.
ACTION 2.2. Progress (in lab-scale fermentation)
13
BIOSTAT® Bplus (Sartorius BBI Systems GmbH, Germany) equipped with a digital control unit DCU
0
20
40
60
80
100
120
0 10 20 30 40 50 60
con
cen
trat
ion
[g/
L]
time [hours]
without nutrients 15 g/L yeast extract
300 mL alfalfa juice 600 mL alfalfa juice
Bread PCP-01: Influence of (green) alfalfa juice
ACTION 2.2. Progress (in pilot-scale fermentation)
14
Comparison of Sugar Bread (PBV-02, 280312) in 600-L- and 1-L-Bioreactor
00
20
40
60
80
100
120
600-L-scale 1-L-scale
SFP 33 SF 1678
con
cen
trat
ion
[g/
L]
Lactate 24h Lactate 48h Lactate End
Starch End Sugars End
This European Project is funded by LIFE+ Programme, under grant agreement LIFE 10 ENV ES 479. This ppt reflects the consortium´s opinion and the European Community is not liable for the use of the information contained herein.
15
Fermentation
Biomass separation
Raw material storage Hydrolysis,
Pre-treatment Pre-, Microfiltration;
Sterilization of nutrient
broth & additives
Softening
Monopolar/Bipolar
Electrodialysis
Ion exchange
Decolorization
Evaporation
Pro
ce
ss
ste
ps
fo
r th
e m
an
ufa
ctu
re o
f la
cti
c a
cid
0
100
200
300
400
500
600
700
800
900
1000
0
500
1.000
1.500
2.000
2.500
End_Ferm MF NF Soft Ed_m Ed_b IC/K IC/A Decol NF_2 IC/K_2 Evap
lacta
te [
g/L]
ion
s [
mg
/L]
SFP 33, without sodium
ACTION 2.3. Progress (in DSP)
16
DSP (small scale) of Sugar Bread (PBV-02, 280312) Lactate from SFP 33
LA: Lactic Acid / (chiral) HPLC
MF/UF/NF: Micro-/Ultra-/Nanofiltration
Soft: Softening
ED: Electrodialysis
(m_monopolar; b_bipolar)
IC/K: Ion exchange/cations
IC/A: Ion exchange/anions
Decol: Decolorization
Evap: Evaporation
08.11.2013 17
Starbucks food waste transformed into bioplastics By Joe Whitworth, 23-Aug-2012
http://www.foodproductiondaily.com/Supply-Chain/Starbucks-food-waste-transformed-into-bioplastics
Starbucks’ food waste sent to a ‘food biorefinery’ has been turned into bioplastics that can
be used in packaging thanks to Hong Kong researchers.
The biorefinery changed food waste such as spent coffee grounds and stale bakery goods from
the retail chain in Hong Kong into succinic acid for making plastics. Carol S. K. Lin led the
research team who developed successful laboratory testing of a biorefinery intended to change
food waste into plastics and other everyday products. Their report on the project came at the
244th National Meeting & Exposition of the American Chemical Society.
Photo credit:
flickr/manuel I MC
Thank you very much for your attention!
With the support of:
Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V.
Max-Eyth-Allee 100, D-14469 Potsdam, GERMANY
Fon: +49(331)5699-112
email: [email protected]
http://de.linkedin.com/pub/joachim-venus/15/276/3b2/
