Embed Size (px)
Citation preview
Lactic acid fermentation
based on residues from
a sugar mill
Joachim Venus
Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V.
Max-Eyth-Allee 100, D-14469 Potsdam, GERMANY
Fon: +49(331)5699-112, email: [email protected]
http://de.linkedin.com/pub/joachim-venus/15/276/3b2/
2
Converting European Sugar Beets into High-Value products
Dr. Timo Koch, Pfeifer & Langen KG
Emil Pfeifer
1806 - 1889
Valentin Pfeifer
1837 - 1909
Eugen Langen
1833 - 1895
Pfeifer & Langen …
… an independent family business since 140 years
3
P&L in Europe
Sugar Production (12 units)
sales office (9 units)
Lage
Appeldorn
Jülich
Könnern
Glinojeck
Sroda
Gostyn
Mieska Gorka
Oradea
Euskirchen
Elsdorf
Radechiv
1927 Experimental farm of the Agricultural University Berlin
1933 Independent research center on agricultural mechanization
1952 Central institute of agricultural engineering of East Germany
1992 Reestablished after the reunification of Germany
Today: Leibniz Institute for Agricultural Engineering Potsdam-Bornim
- member of the Leibniz Association
History
1927 Experimental farm of the Agricultural University Berlin
1933 Independent research center on agricultural mechanization
1952 Central institute of agricultural engineering of East Germany
1992 Reestablished after the reunification of Germany
Today: Leibniz Institute for Agricultural Engineering Potsdam-Bornim
- member of the Leibniz Association
History
Technology
assessment in
agricultural
systems
Technologies and processes for crop
production and livestock management
Research structure
15.10.2014
15.10.2014 6
March 2014 May 2012 2010/2011_en
White Biotechnology - Using renewable resources for industry
Biobased products and processes from renewable resources not only help preserve
the environment and climate,
but also make a significant contribution to the structural change from a petrochemical
to a biobased industry, with related opportunities for growth and employment.
Industrial biotechnology, also known as white biotechnology, is an important
driving force in this transition.
15.10.2014 7
Trends in Biotechnology April 2013, Vol. 31, No. 4
SpecialChem - Aug 20, 2014 http://www.specialchem4bio.com/news/2014/08/20/lactic-acid-market-estimated-to-reach-usd-3577-5-mn-by-2019-marketsandmarkets
The market for lactic acid is growing as it is largely used in various industrial applications such as in
biodegradable polymers, food & beverages, personal care products, and pharmaceutical industries.
The lactic acid market is mainly driven by its end-use industries.
In 2013, Biodegradable polymers formed the largest application for lactic acid, followed by food
and beverages. The lactic acid market is estimated to grow at a CAGR of 18.8% from 2014 to
reach $3,577.5 million by 2019.
15.10.2014 8
Biorefinery-concept for (1st, 2nd, 3rd…?) biomass feedstocks
- BIOCONVERSION -
Vodnar, D.C.; Venus, J.; Schneider, R.; Socaciu, C.:
Chem. Eng. & Technol. 33(2010) 468-474
15.10.2014 9
Top Value Added Chemicals from Biomass
Volume I — Results of Screening for Potential
Candidates from Sugars and Synthesis Gas (August 2004)
15.10.2014 10
Table 1: Overview of chemicals that are currently
produced, or could be produced, from biomass
together with their respective market type, size of
the market, and potential biomass feedstock.
Major players involved are also given.
M.A. Abdel-
Rahman et al.
Journal of
Biotechnology
156 (2011) 286–
301
15.10.2014
Starchy materials (cereals, industrial grade corn/potatoe starch, tapioca)
Green biomass (alfalfa, grass juice, lupine, sweet sorghum, forage rye, silage, coco juice)
Lignocellulosics (wood/straw hydrolysates, 2ndG sugars)
Residues & By-products (bagasse, oilseed cake/meal, thick juice, molasses, whey, coffee residues, waste bread,
waffle residues, algae biomass, fruit residues, meat & bone meal…)
tapioca
bagasse
waste bread
pine
coco juice
2G sugars 2G sugars
1G/2G sugars
green biomass
several residues…
lupine
cereals,
straw
sorghum
Fermentation feedstocks already tested:
silage
algae
biomass
Coffee
residues
The conventional processes for producing lactic acid from
(lignocellulosic) biomass include the following 4 main steps:
15.10.2014 12
(1) Pretreatment—breaking down the structure of the (lignocellulosic) matrix
(2) Enzymatic hydrolysis—depolymerizing lignocellulose to fermentative sugars,
such as glucose (C6) and xylose (C5), by means of hydrolytic enzymes
(3) Fermentation—metabolizing the sugars to lactic acid, generally by LAB
(4) Separation and purification of lactic acid—purification of lactic acid to meet
the standards of commercial applications
Pilot plant facility for lactic acid fermentation at Leibniz-Institute for Agricultural Engineering Potsdam-Bornim / ATB
13
On the Road to Industrial Sustainability
Pfeifer & Langen invests in BioIndustry Research
Converting European Sugar Beets in High-Value Products
Thick juice from sugar beets
Production of thick juice on
industrial scale
All Season available
Sustainable value chain:
„From Field to Factory“
Potential feedstock for chemical
buidling blocks
Lactic acid
Succinic acid
1,4-BDO
…
Thick Juice at a glance
•P&L and ATB evaluate potential of sugar beet thick juice as feedstock for the bioindustry
•P&L is an European sugar producer, ATB is a member of the Leibniz Society
Batch Fermentation set-up
Conditions : Vessel volume: 3 Litre
Temperature: 52°C pH value: 6,0
pH correction: 20% NaOH
Broth composition:
Yeast extract (15 g/L) K2HPO4 (2 g/L) MgSO4 (0,1 g/L) MnSO4 (0,05 g/L)
Carbon sources:
Thick juice (sugar beets) Sucrose (cane-sugar) Maize starch
BIOSTAT®B plus/B/MD equipped with a digital control unit DCU
15.10.2014 15
Comparison of several sugar-based feedstocks
Performance on different carbohydrates (Parallel trials SF1159/1160/1161 – starting from the same inoculum)
0
10
20
30
40
50
60
70
80
90
100
110
0 10 20 30 40 50
Lacta
te [
g/L]
time [hours]
cane-sugar
maize starch
thick juice
0
2
4
6
8
0 10 20 30 40 50
pro
ducti
vit
y [
g·L
-1·h
-1]
time [hours]
cane-sugar
thick juice
maize starch
0
20
40
60
80
100
120
140
0 10 20 30 40 50
Subst
rate
[g/L]
time [hours]
cane-sugar
maize starch
thick juice
Example sugar beets:
e.g. molasses, thick juice
Koch, T.J.; Venus, J.; Bruhns, M.: Sugar beet syrups in lactic
acid fermentation – Part I. Sugar Industry 139(2014) No. 8, 495–
502
Exp. No SF1167 SF1166 SF1168
Substrate Code SB-006 SB-002 SB-005
Total turnover 93% 91% 86%
Yield 77% 74% 74%
Max. Productivity
[g L-1 h-1]
4.90 5.50 4.93
Results of repeating fermentation experiments with
different thick juice sample 0
10
20
30
40
50
60
70
80
90
100
0 10 20 30 40 50
Lacta
te [
g/L]
time [hours]
SF 1167SF 1166SF 1168
Fermentation with
cell retention
Pilot fermentor Type P, 450 L
(Bioengineering AG)
MOLSEP®Hollow fibre PES membrane
(FS10-FC-FUS50E2, MICRODYN-NADIR
GmbH/Daicen Membrane Systems
Ltd.)
Softening 2 x 135 L PUROLITE, 1,5 m³/h (UIT
GmbH Dresden)
Monopolar/Bipolar
Electrodialysis
FT–EDR/ ED4–15; 7,68 m2
monopolar/3,2 m2 bipolar (FuMA-Tech
GmbH Vaihingen)
Ion exchange Cationic resin, 50 L; Anionic resin,
2 x 90 L (UIT GmbH Dresden)
Decolorization Activated carbon, several specific
resins
Evaporation
«chemReactor» CR15 (Büchi AG
Uster/Switzerland);
Rotary Evaporator LABOROTA 20 S
(Heidolph Instruments)
18
Dow
nst
ream
pro
cess
ing o
f ra
w lacta
tes
& lacti
c a
cid
Enantiopurity
of strain A35
@ 52°C
0
20
40
60
80
100
120
ThJ Sugar ThJ Sugar ThJ Sugar ThJ Sugar ThJ Sugar
g/L
97,0
97,5
98,0
98,5
99,0
99,5
100,0
L/D
[%]
L(+)D(-)L/D [%]L/D [ee]
15.10.2014 20
What’s coming next…?
In the previous work (Part I) the use of sugar beet thick juice in lactic acid fermentation was
reported. The discussed studies showed the general benefits of choosing thick juice as a substrate in
lactic acid in comparison with crystalline sucrose or corn starch. In the present work the
investigations are expanded to raw juice, another intermediate product in sugar beet
processing. As raw juice is generated earlier in the value chain than thick juice its use in
fermentation processes is also, from an economic aspect, of great interest.
15.10.2014 21
The Copenhagen Declaration
for a Bioeconomy in Action
…
9. The conference also underlined the
need for new pilot and demonstration
plants and scaling up facilities, in
particularly biorefineries. It was stressed,
that the development of these facilities requires
smart integration of various funding sources,
including the Common Agricultural Policy, the
Common Fisheries Policy, the Cohesion Policy,
the Renewable Energy Policy, Horizon 2020, and
private investments.
…
Copenhagen conference “Bioeconomy in Action” on 26 March - 28 March 2012
Universities, Research Institutes, SMEs
Applied & basic research
Industry Industrial application
Large-scale production
Carus/Carrez/Kaeb/Ravenstijn/Venus: Level Playing Field for Bio-based Chemistry
and Materials. – bioplastics MAGAZINE [03/11] Vol. 6, 52-55
Scale-up of bioprocesses
15.10.2014 23
Pilot plant facility • pilot facility for production of lactic acid at the ATB consequently fills a gap in the
various phases of bioprocess engineering
• provision of product samples is intended to open up
the possibility of interesting partners in industry with
specific product requirements in various applications
BIOSTAT® Bplus (Sartorius BBI Systems GmbH, Germany)
equipped with a digital control unit DCU for the
continuous fermentation with cell recycling
scale up
Pilot fermentor Type P, 450 L (Bioengineering AG) Venus, J.; Richter, K.: Eng. Life Sci. 2007, 7, No. 4, 395-402
15.10.2014 24
Pilot plant for „Fermentation Process Improvement“
Opportunities & Challenges …
starchy materials,
lignocellulosics, residues & by-
products, green biomass
feedstock
sugars, hydrolyzates
press juice…
fermentation, down-stream
pretreatment bioconversion
(raw)lactate, lactic
acid, biomass...
…bioplastics
products
Venus, J.: Biotechnol. J. 1(2006) No. 12, 1428–1432
15.10.2014 25
Projects & Partners: FNR joint project SynRg (FKZ 22023008)
AiF ZIM/KF (FKZ 2050812ST0 & FKZ 2050813SB1)
International cooperation in education & research with Brazil (DLR BRA 08/A02 & FKZ 01DN12053)
International cooperation in education & research with Russia (DLR RUS 10/128)
COST-Aktion TD1203 "Food waste valorisation for sustainable chemicals, materials & fuels (EUBis,
http://costeubis.org/)" – MC Member
EU-Project BREAD4PLA, LIFE10 ENV/ES/479 (http://www.bread4pla-life.eu/index.php)
EU-Project CELLULAC, CIP-EIP-Eco-Innovation-2011 (GA304523,
http://cordis.europa.eu/projects/rcn/108946_en.html)
Scientific exchange programme BMBF/DLR with Colombia (CACHIPLA / FKZ 01DN13056 &
CAFELACTIC / FKZ 01DN13057)
Contract research / bilateral assignments (among others UIT GmbH Dresden; NordBioChem Tallinn;
Sustainable BioPoloymers Ltd.& NUI Galway; HF Biotec Berlin GmbH; American Science and
Technology Chicago; Stenger Waffelfabrik GmbH; Cenicafe-FNC Colombia; Hermetia Baruth GmbH)
Thank you very much for your attention!
With the support of:
Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V.
Max-Eyth-Allee 100, D-14469 Potsdam, GERMANY
Fon: +49(331)5699-112
email: [email protected]
http://de.linkedin.com/pub/joachim-venus/15/276/3b2/
