Uses of the CCD

Image fluorescence - living or fixed, multiple colors

Image phase or DIC - living or fixed

Elements of the CCD workstation (located in Rm 012)

Microscope CCD camera Computers for image aquisition/analysis Filter wheels Acquisition/analysis software- Openlab CO2 incubator and heating stage Focus drive

Elements of the CCD workstationLeica DMIRB - inverted microscope

20X phase - adjustable focal length - to be used with anything from thick petri plastic to thin (0.17um) coverslips

40X phase- adjustable focal length - 63X phase- adjustable focal length - 63X water immersion (no phase) Oil immersion objectives can be shared with the

confocal

Elements of the CCD workstationCCD camera

Princeton Instruments MicroMax 12-16 bit, 1000X800 pixels Back illuminated, cooled CCD High resolution/high sensitivity

Elements of the CCD workstationComputers for image acquisition and analysis

Acquisition computer (Mac G3, 256 MB RAM, 4GB) is located in room with camera - data should be transferred to CD or the analysis computer soon after acquisition because files are large.

Analysis computer is located in Room 123

Elements of the CCD workstationFilter Wheels

Emission/Excitation Filter Wheels Filters Include: FITC CY3/Texas Red GFP BFP

Elements of the CCD workstationMicroscope-attached CO2 incubator and heating stage

Allows long-term experiments at constant temperature and % CO2

Elements of the CCD workstationAcquisition and Analysis Software - Openlab

Macros control camera/focus drive/filter wheels for long-term acquisition

Analysis package allows manipulation of images for presentation and quantitation

Elements of the CCD workstationAcquisition and Analysis Software - Openlab

Elements of the CCD workstationFocus Drive

Allows acquisition from multiple focal planes - can compensate for movement of living structures

Can be used to obtain confocal images in conjunction with the Silicon Graphics program Imaris

Elements of the CCD workstationFocus Drive

QuickTime™ and aVidéo decompressor

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QuickTime™ and aVideo decompressor

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