Osteoblast UTM
1) Culture Osteoblast
a) Cell line and mediumItemsProduct details
NHOst-Osteoblasts OGM, cryo amp,CC-2538Lonza(Choice Care)
One Normal Human Osteoblast Cell Medium BulletKit 500 ml
Clonetics OGM BulletKit (CC-3207) contains one 500 ml bottle of
Osteoblast Basal Medium (OBM) and OGM SingleQuots containing the
following growth supplements: FBS, 50 ml; Ascorbic Acid, 0.5 ml;
GA-1000, 0.5 ml.CC3207Lonza
(Choice Care)
TrypLE Express Enzyme (1X), no phenol red100ml12604-013Gibco
Life Tech
b) Cryopreservation ItemsProduct details
Phosphate Buffered Saline tablet
Product Number:E404-100TABS3-4 weeks
Dimethylsulfoxide
Product Number: 0231-500ML3-4 weeks
Sodium Bicarbonate0865-500G3-4 weeks
Trypan Blue stain 0.4% 100ml15250-061Gibco Life Tech
2) Osteoblast Test a) ResazurinResazurin Test materialProduct
Details
Resazurin stockIn VitroToxicology Assay Kit, Resazurin
basedTOX8-1KT
b) ALPALP assayProduct Details
M-PER Mamalian protein extractionReagent250 mL0078501Life
Technologies
P-nitrophenyl phosphate tablet-lkit105
tablets(includeDiethanolamide 5*Buffer225 mL)37620
c) FESEMFESEMProduct Details
Glucoaldehyde0875-100ML3-4 weeks
3) Consumable itemsConsumables and materialsProduct Details
serological pipettecapacity 10mL, individually wrapped,
paper/poly, sterile
SP8100-12-3 weeks
cryogenic vialssize 2.0mL, sterile, external threads
SPL 430122-3 weeks
culture flasks, tissue culture treatedsurface area 75cm2, canted
neck
SPL 701752-3 weeks
tissue culture plates96 well plate, flat bottom, polystyrene,
0.34cm2, sterile
SPL 300962-3 weeks
tissue culture plates24 wells, flat bottom, polystyrene,
1.86cm2, sterile, 64/cs
SPL 300242-3 weeks
Freezing container, Mr. Frosty
KR-FB-184-8 weeks
pipette tipsvolume range 1000microliter, universal, bulk
packTR-1000-B*1-2 weeks
pipette tipsvolume range 200microliter, universal, bulk pack
TR-200-Y*1-2 weeks
microcentrifuge tube, 1.5 mL with lid, PPtransparent, pack of
500
CT0150-B-N*1-2 weeks
Transfer pipette, polyethylenegeneral purpose, standard, bulb
draw 3.2mL, sterile
PP300-12-3 weeks
