Upload
trinhdan
View
217
Download
2
Embed Size (px)
Citation preview
Supplemental Material:
Supplement table 1: Physico-chemical parameters of untreated and treated wastewaters were analysed according to APHA standard methods
protocol.
Parameters Petrochemical
inlet to ETP
Petrochemical
outlet from ETP
Petrol refinery
Inlet to ETP
Petrol refinery
Outlet from
ETP
Coke
oven Inlet
to ETP
Coke oven
outlet from
ETP
Tolerance limit
for Effluent std,
BIS
Tolerance limit
for Drinking
water
pH 9.1 6.6 9.1 6.8 8.50 7.10 5.5-9.0 6.5-8.5
Temperature [°C] 33.0 31.0 36.0 31.0 32.00 28.70 40 -
Dissolved oxygen [mg/l] 3.3 1.2 3.3 1.8 3.17 1.42 - -
Theoretical COD [mg/l] 3240 180 4540 261.0 810.30 225.39 250 -
HeadspaceBOD [mg/l] 55 10 200 31.0 92.30 21.90 30 -
Oil and grease [mg/l] 2.2 2 34.3 28.4 2.23 1.02 10 .01
Dissolved solids [mg/l] 3243.45 2062 3400 2100 1287.82 945.00 2100 500
Ammonical nitrogen [mg/l] 533.90 48.2 658.0 98.0 562.30 89.93 50 100
ParaNitrate nitrogen [mg/l] 56.16 10.7 66.16 46.0 55.39 23.21 - 45
Total hardness [mg/l] 518.19 272.87 650.19 305.0 495.39 140.21 - 300
S Phenol(mg/l) 128.47 0.95 142.47 1.05 123.60 0.60 1.0 0.001
Cyanide [mg/l] 1.3 0.18 1.9 0.2 20.70 0.20 0.2 0.05
A
B
Supplement Figure 1: A) Schematic of the studied petrochemical industry effluent treatment plant. B) Schematic of the studied steel industry effluent treatment plant.
1 2 3 4 5
Supplement Figure 2: DNA fragmentation of genomic DNA isolated from cultured cells
treated with the Petrochemical sludge extract. A representative result of three sets of
experiments is shown. Lane 1: Positive control benzo[a]pyrene; Lane 2: sludge extract 200 μl;
Lane 3: sludge extract 100 μl; Lane 4: sludge extract 50 μl; Lane 5: negative control (DMSO);
Lane 6: Molecular weight marker.
Supplement Figure 3: Immunoblot analysis showing P53 protein accumulation in cell
cultures exposed to petrochemical sludge extract. Lane 1: negative control; Lane 2: sludge
extract 50 μl; Lane 3: sludge extract 100 μl; Lane 4: sludge extract 200 μl; Lane 5: positive
control benzo[a]pyrene
Supplement Figure 4: DNA fragmentation of genomic DNA isolated from cultured cells
treated with the Petrochemical effluent water extract. A representative result of three sets of
experiments is shown. Lane 1: Molecular weight marker; Lane 2: Control (DMSO); Lane 3:
effluent extract 50 μl; Lane 4: effluent extract 100 μl; Lane 5: effluent extract 200 μl and Lane
6: Positive control benzo[a]pyrene
Supplement Figure 5: Immunoblot analysis showing P53 protein accumulation in cell
cultures exposed to petrochemical effluent water extract Lane 1: negative control; Lane 2:
effluent extract 50 μl; Lane 3: effluent extract 100 μl; Lane 4: effluent extract 200 μl; Lane 5:
positive control benzo[a]pyrene
1 2 3 4 5
Supplement Figure 6: DNA fragmentation of genomic DNA isolated from cultured cells
treated with the petroleum refinery sludge extract. A representative result of three sets of
experiments is shown. Lane 1: Molecular weight marker; Lane2: Control (DMSO); Lane 3:
sludge extract 50 μl; Lane 4: sludge extract 100 μl, Lane 5: sludge extract 200 μl; Lane 6:
Positive control benzo[a]pyrene
Supplement Figure 7: Immunoblot analysis showing P53 protein accumulation in cell
cultures exposed to petroleum refinery sludge extract. Lane 1: negative control; Lane 2:
sludge extract 50 μl; Lane 3: sludge extract 100 μl; Lane 4: sludge extract 200 μl; Lane 5:
positive control benzo[a]pyrene
Supplement Figure 8: DNA fragmentation of genomic DNA isolated from cultured cells
treated with the petroleum refinery effluent water extract. A representative result of three sets
of experiments is shown. Lane 1: Molecular weight marker; Lane 2: Control (DMSO); Lane
3: effluent extract 50 μl; Lane 4: effluent extract 100 μl; Lane 4: effluent extract 100 μl, Lane
5: effluent extract 200 μl and Lane 6: Positive control benzo[a]pyrene
Supplement Figure 9: Immunoblot analysis of P53 expression in cell cultures exposed to
petroleum refinery effluent water extracts. Lane 1: negative control; Lane 2: effluent extract
50 μl; Lane 3: effluent extract 100 μl; Lane 4: effluent extract 200 μl; Lane 5: positive control
benzo[a]pyrene
Supplement Figure 10: DNA fragmentation of genomic DNA isolated from cultured cells
treated with coke oven wastewater extract before treatment. A representative result of three
sets of experiments is shown. Lane 1: Positive control benzo[a]pyrene; Lane 2: water extract
50 μl; Lane 3: water extract 25 μl; Lane 4: water extract 10 μl, Lane 5: Control (DMSO) and
Lane 6: Molecular weight marker
Supplement Figure 11: Immunoblot analysis of P53 expression in cell cultures exposed to
coke oven wastewater extract before treatment. Lane 1: positive control benzo[a]pyrene; Lane
2: water extract 50μl; Lane 3: water extract 25 μl; Lane 4: water extract 10μl; Lane 5:
negative control.
Supplement Figure 12: DNA fragmentation of genomic DNA isolated from cultured cells
treated with the coke oven effluent water extract after treatment. A representative result of
three sets of experiments is shown. Lane 1: Molecular weight marker; Lane 2: effluent extract
10 μl; Lane 3: effluent extract 25 μl; Lane 4: effluent extract 50 μl, Lane 5: Control (DMSO)
and Lane 6: Positive control benzo[a]pyrene
Supplement Figure 13: Immunoblot analysis of P53 expression in cell cultures exposed to coke
oven effluent water extract after treatment. Lane 1: negative control; Lane 2: effluent extract 10
μl; Lane 3: effluent extract 25 μl; Lane 4: effluent extract 50 μl; Lane 5: positive control
Benzo[a]pyrene.
Supplement Figure 14: Cytotoxicity assay A. Sludges Extract (Petrochemical industy) B. Watewater Extract (Petrochemical industry) C. Sludges Extract (Petro refinery) D. ETP Sludges Extract (Petro refinery) E. Watewater Extract (Petro refinery) F. Watewater Extract (Steel industry before treatment) G. Watewater Extract (Steel industry after treatment)