Supplemental Information

Plant cytokinesis: terminology for structures and processesAndrei Smertenko1*, Farhah Assaad2, František Baluška3, Magdalena Bezanilla4#, Henrik Buschmann5, Georgia Drakakaki6, Marie-Theres Hauser7, Marcel Janson8, Yoshinobu Mineyuki9, Ian Moore10, Sabine Müller11, Takashi Murata12, Marisa S. Otegui13, Emmanuel Panteris14, Carolyn Rasmussen15, Anne-Catherine Schmit16, Jozef Šamaj17, Lacey Samuels18, Andrew Staehelin19, Daniel Van Damme20,21, Geoffrey Wasteneys18, Viktor Žárský22

 1Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA2Botany Department, WZW, Technische Universität München, Freising, 85354 Germany3Department of Plant Cell Biology, IZMB, University of Bonn, Kirschallee 1, D-53115, Bonn, Germany4Biology Department, University of Massachusetts, Amherst, MA 01003, USA5Botany Department, School of Biology and Chemistry, Osnabrück University, Barbarastraße 11, 49076 Osnabrück, Germany6Department of Plant Sciences, University of California Davis, Davis, CA 95616, USA7Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria8Laboratory of Cell Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, the Netherlands9Department of Picobiology, Graduate School of Life Science, University of Hyogo, Shosha 2167, Himeji 671-2201, Japan10Department of Plant Sciences, University of Oxford, South Parks Rd., Oxford, OX1 3RB, UK11Center for Plant Molecular Biology, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany12National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan13Departments of Botany and Genetics, Laboratories of Cell and Molecular Biology, University of Wisconsin-Madison14Department of Botany, School of Biology, Aristotle University of Thessaloniki, Thessaloniki GR-541 24, Macedonia, Greece15Center for Plant Cell Biology, Institute of Integrative Genome Biology, Department of Botany and Plant Sciences, University of California, Riverside, California, USA, ORC-ID 0000-0002-4354-629516Institut de biologie moléculaire des plantes, Centre National de La Recherche Scientifique, Université de Strasbourg, F67084, Strasbourg-cedex, France17Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic18Department of Botany, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada19Department of Molecular, Cellular and Developmental Biology, University of Colorado, UCB 347, Boulder, CO 80309-0347, USA20Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; 21Center for Plant Systems Biology, VIB, Technologiepark 927, 9052 Ghent, Belgium

22Department of Experimental Plant Biology, Charles University and Institute of Experimental Botany, ASCR, Prague, Czech Republic#Current address: Department of Biological Sciences, Dartmouth College, Hanover, NH 03755-3529, USA*Correspondence: andrei.smertenko@wsu.edu InventoryFigure S1. Relates to Figure 1 and 2 and shows cartoon of key stages of the plant cell division and microscopy images of the preprophase band. Figure S2. Relates to Table 1 and shows several representative examples of the localization of cytokinetic markers. Figure S3. Relates to Figure 1 and shows immunostaining of the phragmoplast and cartoons of three-dimensional phragmoplast morphology at the successive stages of cytokinesis.  

 Figure S1. Preprophase band formation.A, PPB forms during G2 and overlaps with the beginning of prophase. Phragmoplast forms during telophase and disappears at the end of cytokinesis. In large plant cells cytokinesis can continue into G1 phase.B, PPB in onion apical root meristem cells appears as a wide array of aligned microtubules and actin filaments. As chromosomes condense, PPB narrows down to form a tight band of microtubules and actin filaments. Then actin filaments become depleted from the PPB region forming actin depletion zone (marked with the arrowhead). Immunolabelling of microtubules with anti-tubulin (green), actin filaments with anti-actin (red), and staining of nucleus with Hoechst 33258 (blue; modified from Takeuchi, M., and Mineyuki, Y. 2014. Actin-microtubule interaction during preprophase band formation in onion root tips visualized by immuno-fluorescence microscopy. In: Atlas of Plant Cell Structure, Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.(eds), Springer Japan, Tokyo, 132-133.).

 Figure S2 . Localization of fluorescent markers for different cytokinetic regions and structures.Arrows indicate: cortical division zone in GFP-TPLATE, YFP-POK1; cell plate fusion site in GFP-AIR9 image; and phragmoplast midzone in GFP-katanin images.  

 Figure S3. Stages of phragmoplast expansion.A, Staining of phragmoplast dwith anti-tubulin (green) and nuclei with DAPI (blue) in tobacco BY-2 cells. Scale bar, 5 um. Arrowheads indicate phragmoplast attachment site to the plasma membrane. Note depolymerization of microtubules at the attachment sites.B, Cartoons showing three-dimensional phragmoplast morphology at the stages shown in A.