Skin Moon®

Proven Skin-Brightening Effect From Plant Extracts

INCI Name: Citrus Sinensis (Orange) Fruit Extract, Oryza Sativa (Rice) Extract, Capparus Spinosa Fruit Extract, Olea Europaea (Olive) Leaf Extract, Maltodextrin

BackgroundAn umbrella can protect skin against sunlight-induced pigmentation, but it is not enough because hyperpigmentation is often produced by several factors, including aging, hormonal changes, chronic skin irritation, inflammation or other diseases. In many parts of the world, such as Asia, “moon-like” face skin means beauty and charm. Skin MoonSkin Moon®® is a new combination of extracts with a high skin brightening activity. It is a natural ingredient designed to safely lighten skin tone.

What it DoesSkin MoonSkin Moon®® is able to inhibit skin melanogenesis, showing an activity similar to hydroquinone and higher

than kojic acid and arbutin, which are ingredients currently used in skin-brightening products. Skin Moon® is also well-tolerated by the skin and does not cause skin sensitivity, unlike other agents commonly used in skin de-pigmentation(1).

Skin MoonSkin Moon®® can be used for skin-brightening and anti-aging products. In contrast with other agents (hydroquinone and kojic acid), Skin MoonSkin Moon®® can be applied during sun exposure because it does not have a cutaneous photo-sensitizing effect.

Skin Moon is a registered trademark of Bionap srl.

References1. Rizza L, et al., Skin-whitening effects of Mediterranean herbal extracts by in vitro and in viva models. J Cosmet Sci 63 (5): 311 -320 (2012).

www.BotanicalsPlus.com

Key Benefits:• Inhibits melanogenesis• Skin brightener• Non-photosensitizing effect

Product Applications:• Skin care creams, gels, and lotions• Sensitive skin care products• Sun and after sun care products• Anti-aging products• Skin brightening products

TECHNICALBULLETIN

COMPOSITION Skin Moon®

(% W/W)Capparis spinosa extract 15 – 20Olea europaea extract 15 – 20Red orange extract 10Oryza sativa extract 10Total polyphenols (HPLC method) 2 – 3

Typical PropertiesForm SolidOdor CharacteristicColor Yellow-BrownpH (2.5 aqueous solution) 3.5 - 6.5

Solubility Moderately soluble in water

Total polyphenols 2 - 3Recommended Use Level 2 - 3% w/w

Clinical StudiesIn vitro In vitro Evaluation of Anti-Tyrosinase ActivityEvaluation of Anti-Tyrosinase ActivityThe experimental protocol was as follows: Spectrophotometric determination of dopachrome produced from L-DOPA + tyrosinase. Determination of the IA% (Inhibitory activity percentage of tyrosinase) of Capparis spinosa, Olea Europaea, Citrus sinensis, and Oryza sativa extracts and their blend in comparison to kojic acid and hydroquinone.

ResultsAll herbal extracts showed tyrosinase inhibitory activity. A synergistic effect was observed when they were combined in the Skin MoonSkin Moon® ® ingredient, with higher activity than kojic acid and hydroquinone.

Evaluation of Skin Whitening EffectEvaluation of Skin Whitening EffectThe experimental protocol was as follows: 15 healthy volunteers (aged 30-45 years). Induction of skin tanning and application of skin whitening formulations for 4 weeks. Evaluation of skin melanin index (M.I.) by reflectance spectrophotometry. Depiction of method:

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0Kojic acid Hydroquinone Capparis Citrus Oryza Olea Skin Moon®

spinosa sinensis sativa europaea

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Anti-Tyrosinase ActivityAnti-Tyrosinase Activity

2. Application of skin-whitening formulations: HY: 3% Hydroquinone KJ: 3% Kojic acid SM: 3% Skin Moon®

3. Evaluation of Melanin Index by reflectance spectrophotometry for 4 weeks on each skin site.

1. Skin tanning induced sun lamp exposure

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Trend of Melanin Index (M.I.)During Skin-Whitening Treatments

Hydroquinone Kojic acid Skin Moon®

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Skin-Whitening Effect

ResultsSkin MoonSkin Moon® ® showed a skin-whitening effect similar to hydroquinone and higher than kojic acid.

Evaluation of Skin Photosensitizing EffectEvaluation of Skin Photosensitizing EffectThe experimental protocol was as follows: 15 healthy volunteers (aged 30-45 years). Application of tested formulations once daily for 4 weeks. Induction of skin erythema by UV lamp exposure and evaluation of erythema index (E.I.) by reflectance spectrophotometry. Depiction of method:

1. Treatment with tested formulations (once a day for 4 weeks); HY: 3% Hydroquinone KJ: 3% Kojic acid SM: 3% Skin Moon®

2. UV Lamp TreatmentEach skin site was treated with a UV lamp emitting a range of 290-320 nm for double of the minimal erythema dose (MED) of each subject.

3. UV-Induced Erythema Evaluation For each skin site UV-induced erythema was quantified by reflectance spectrophotometry.

HY KJ SMHY KJ SM

HY KJ SM

Results SummarySkin MoonSkin Moon®® provided similar or better efficacy results as hydroquinone and kojic acid with much lower photosensitization.

Hydroquinone Kojic acid Skin Moon®

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PhotosensitivityResultsSkin MoonSkin Moon® ® was shown to induce much reduced photosensitizing effects compard to hydroquinone and kojic acid.

163 East Main Street, Suite #273Little Falls, New Jersey 07424 ─ USAtel: (973) 439-1417 info@BotanicalsPlus.com www.BotanicalsPlus.com

Skin Moon TB ver 1.1