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Tulip Biolabs, Inc.PO Box 334, West Point, PA 19486 USA

Phone/Fax: 610.584.2706info@tulipbiolabs.comwww.tulipbiolabs.com

©2009 Tulip Biolabs, Inc.

Proteins andAntibodies for

Poly(ADP-ribose)Research

Special standard!PARP1 Automodified,

human protein

Detect cleavage!Anti-PARP1, whole protein

IgY polyclonal

Great Sensitivity!Anti-poly(ADP-ribose)

IgY polyclonal

Classic antibody!Anti-poly(ADP-ribose)

Clone 10H

Gotowww.tulipbiolabs.com

formoreinfo!

Purified, lyophilized!PARP1, human,

highly active enzyme

NEW

PRODUCTS!

NEW!

NEW!

Anti-PARP1, whole proteinChicken polyclonal antibodyDeveloped at Tulip Biolabs using highly purified, recombinant full-length human PARP1 as immunogen. Recognizes intact PARP1 MW=113kDa, the 89kDa apoptosis-induced cleavage product, and other lower molecular weight fragments of PARP1 (see datasheet). Useful for detecting apoptotic and necrotic fragments of PARP1 by WB. Recognizes human, rat, and mouse.Order Catalog #105150 µg/$60 200 µg/$175 1 mg/$700

Anti-pADPr, clone 10HMouse monoclonal antibodyRecognizes poly(ADP-ribose), developed and originally described by H. Kawamitsu et al. (1984) Biochemistry 23, 3771. Uses for the clone 10H monoclonal antibody have been described extensively in the literature, and include WB, immunostaining, ELISA-based PARP activity screen, and immunodot blot.Order Catalog #102050 µg/$60 200 µg/$175 1 mg/$700

Anti-pADPr, IgYChicken polyclonal antibodyDeveloped at Tulip BioLabs using purified poly(ADP-ribose) polymer as immunogen. Useful for WB, ELISA, and immunohistochemistry (see publications listed on the datasheet). This antibody has great sensitivity.Order Catalog #102350 µg/$60 200 µg/$175 1 mg/$700

Now Available fromTulip BioLabs...

Proteins and Antibodies for Poly(ADP-ribose) Research!

Ordering Information

EASY-TO-ORDERPlease phone, fax, e-mail, or mail your order to the attention of customer service. Please supply your name, purchase order number, shipping address, billing address and telephone number. Order by credit card online at www.tulipbiolabs.com!

LIMITATIONSAll products are available for research use only and are not approved for use in humans or for clinical diagnosis.

Please contact us for special orders and for pricing on bulk quantities. Discount of 20% for orders of 5 or more of a single size and item.

Tulip BioLabs, Inc.PO Box 334, West Point, PA 19486 USA

Phone/Fax: 610.584.2706info@tulipbiolabs.comwww.tulipbiolabs.com

PARP1 Enzyme, humanHighly active, lyophilized proteinPoly(ADP-ribose) polymerase-1 (PARP1) catalyzes the NAD(+)-dependent addition of ADP-ribose polymers on a variety of nuclear proteins. The Tulip Biolabs, Inc. PARP1 Enzyme, Cat. #2090, is highly purified human PARP1, expressed in a baculovirus expression system. The PARP1 has a high specific activity, ~20000 U/mg (1U=10 fmol ADP-ribose incorporated into immobilized histone in 30 min @ RT). Each vial contains 50 µg PARP1 (approx. 1000 Units). Use in high-throughput enzyme activity/inhibition assays, poly-ADP-ribosylation of target proteins, and other assays.Order Catalog #2090 50 µg/$200

NEW! ANTIBODIESPROTEINS

PARP1 Automodified, humanLyophilized proteinPARP1 Automodified is highly purified human PARP1, expressed in a baculovirus expression system, to which NAD+ and cofactors have been added to allow complete poly ADP-ribosylation of PARP1. The automodified PARP1 is lyophilized from its native state. Useful as a standard in several assay types.Order Catalog #2095 5 µg/$90

NEW!

SDS-PAGE/Coomassie Blue staining of poly(ADP-ribose) automodified PARP1. PARP1 was incubated with 0, 50µM, or 1mM NAD for 10 min@RT in reaction buffer (see pro toco l ) . Samples (0.625µg/lane) were run on SDS-PAGE and stained with Coomassie Blue. With increasing NAD concentrations, the 113kDa PARP1 band decreases and there is a shift to a higher MW smear, indicating poly(ADP-ribose) PARP1 modified to various extents. At longer incubation times, there is a complete conversion from native to poly ADP-ribosylated PARP1 (not shown).