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2

Proposal to Release TX02A0252 as TAM 113 Hard Red Winter Wheat

Jackie Rudd, Ravindra Devkota, Jason Baker, Gary Peterson, Amir Ibrahim, David

Worrall, Mark Lazar, Russell Sutton, Lloyd Rooney, Lloyd Nelson, Brent Bean, Robert

Duncan, Brad Seabourn, Robert Bowden, Yue Jin, and Robert Graybosch

Introduction

TX02A0252, developed by Texas AgriLife Research, is an awned, semi-dwarf,

hard red winter what (Triticum aestivum L.) that is white glumed, medium height and

medium maturing. It is adapted to the High Plains of Texas and similar areas in adjacent

states. TX02A0252 has been extensively tested throughout the Great Plains and is

resistant to the prevalent races of leaf and stripe rust. It has good milling and baking

characteristics with a standard test weight of around 60 lb/bu and relatively strong dough

characteristics. TX02A0252 has a similar area of adaptation and grain yield potential as

TAM 111 and TAM 112. In comparison to TAM 111, it has better bread-making quality

and better leaf rust resistance. In comparison to TAM 112, it has better leaf rust and stripe

rust resistance.

Authorized seed classes of TX02A0252 in the U.S. will be Breeder,

Foundation, Registered, and Certified. An application for Plant Variety Protection

will be made.

Breeding History

TX02A0252 is an F5 derived line from the cross TX90V6313/TX94V3724 made

in 1995 at the Texas AgriLife Research and Extension Center in Vernon. The pedigree of

3

TX90V6313 is TAM 200”S”/TX78A3345-V34 (MV61-06/TAM 105”S”). The pedigree

of TX94V3724 is U1254-1-8-1-1 (TAM 200*4/TA2460, T. tauschii)/TAM 202. The F3 -

F5 generations were grown as bulk populations on the Texas AgriLife Research farm at

Bushland in 1998 (year of harvest), 1999, and 2000. Random heads were harvested from

the F5 population and were planted as head-rows at Bushland in the fall of 2001. The line

that became TX02A0252 was visually selected for its agronomic characteristics and was

grown as a single plot in 2002 and in replicated trials thereafter. Grain yield, test weight,

end-use quality, and disease resistance were the primary selection criteria. It was tested in

Texas Elite trials (TXE) 2006-2007, Southern Regional Performance Nursery (SRPN)

2007-2008, the Texas Uniform Variety Trial (UVT) 2008-2010, and the Wheat Quality

Council (WQC) 2009.

Seed increase started in the fall of 2006 by planting 48 head-rows in Yuma,

Arizona. These were visually evaluated for uniformity and 4 were eliminated because

they were 6 inches taller. The remaining 44 rows were harvested in bulk. This seed was

used to plant one acre in the fall of 2007 and this harvest was used to plant 10 acres in

2008. A late spring freeze in the Rolling Plains devastated the majority of the 2008-2009

wheat crop, including the TX02A0252 increase, so remnant seed from the 2007 planting

was used to plant a one-acre increase in Yuma, Arizona in the fall of 2009. Texas

Foundation Seed Service will be producing Foundation seed during the 2010-2011

season.

Variants observed in the 2009-2010 seed increase included plants that were 4-6

inches taller with white glumes (0.01% frequency) and plants 4-6 inches taller with red

4

glumes (0.001% frequency). The variants were rogued but may occur at a low percentage

(less than 0.01%) in future generations.

Yield

Four years (2007-2010) of grain yield data from Texas AgriLife Research trials in

the High Plains are presented in Table 1 (irrigated) and Table 2 (dryland). Grain yield

from individual location-years and a four-year summary are on each table. TX02A0252

had a similar grain yield as TAM 111 and TAM 112 in irrigated (Table 1) and dryland

(Table 2) trials. TAM 111 and TAM 112 are the two most widely grown cultivars in the

Texas High Plains and they are almost always among the top yielding cultivars. During

the same 4 years, TX02A0252 was tested at other locations in Texas, where the grain

yield was average in the Rolling Plains and below average in northeast, central, and south

Texas locations (UVT data available at http://varietytesting.tamu.edu/wheat/index.htm).

Forage trials conducted at 5 locations across Texas in 2010 indicate that

TX02A0252 has average to good forage production and re-growth after clipping (data

available at

http://varietytesting.tamu.edu/wheat/docs/forageTrials/2010/Forage%20Pub.pdf) and

dual purpose trials conducted in Oklahoma indicate good grain yield after grazing

(unpublished data from Brett Carver, Oklahoma State U.); thus, TX02A0252 can be used

in a dual-purpose (grazing plus grain) system.

5

Agronomic data

The test weight and kernel size of TX02A0252 is similar to TAM 111 and TAM

112 (Tables 1-2). Days-to-heading is similar to TAM 111 and 3-4 days later than TAM

112. Plant height is about 3 cm shorter than TAM 111 and about the same as TAM 112.

Significant lodging and shattering occurred on some cultivars at 3 of the High Plains

irrigated location-years, but almost none were observed on TX02A0252, TAM 111, or

TAM 112 (Table 1). Anecdotal observations indicate that TX02A0252 has a straw

strength similar to TAM 111 and stronger than TAM 112. We normally get an estimate of

winter hardiness from northern state participants in the SRPN, but no differential winter-

kill was reported in either 2007 or 2008. No winter injury was observed on TX02A0252

or on any of the check cultivars during the 4 years of yield trials in Texas.

Disease Resistance

TX02A0252 has good resistance to leaf rust, stripe rust, and stem rust (Tables 3

and 5). USDA-ARS testing postulated that it has the gene Lr24 for leaf rust (Puccinia

triticina) resistance (Table 5); but since it has good adult plant resistance at locations

throughout Texas (Table 3), where Lr24 virulence is known to occur, it must have

additional seedling or adult plant resistance genes (SRPN molecular marker data

indicates Lr34 might be present, data not presented). TX02A0252 was resistant to the

prevalent races of stripe rust (Puccinia striiformis f. sp. tritici) during natural epidemics

in 2007 and 2010. Tests have shown that the 2010 epidemics observed in Castroville and

College Station were due to a new race that had not previously been detected in the Great

Plains. Many wheat cultivars that were resistant in previous years were susceptible to this

6

new race. Both TX02A0252 and TAM 111 were resistant to this new race as well as to

the prevalent races of 2007 and 2009 (Table 3). TX02A0252 is highly resistant to the

most prevalent race, QFCS, of stem rust pathogen (Puccinia graminis f. sp. tritici) in

Texas and the U.S. In seedling tests with multiple stem rust races, TX02A0252 exhibited

resistance to all races of U.S. origin, and race TTKSK (or Ug99 of Kenyan origin) (Table

5). Moderately high infection types were observed when TX02A0252 was tested against

race TTKST, a race in the TTKS lineage that is virulent on Sr24, indicating that Sr24 is

present. TX02A0252 was highly resistant in field stem rust nursery in St. Paul, MN that

was inoculated with a composite of U.S. stem rust races (QFCS, QTHJ, RCRSC, RKQQ,

and TPMK) (Table 5).

SRPN data indicates that TX02A0252 might have some tolerance to acid soils but

it is susceptible to soil borne mosaic wheat virus. Data on wheat streak mosaic virus

resistance is not available. Like TAM 111, it is susceptible to greenbug, Hessian fly, and

Russian wheat aphid.

Quality

TX02A0252 has been tested for 4 years by the Texas AgriLife Research Wheat

Quality Laboratory, 3 years by the USDA-ARS Hard Winter Wheat Quality Laboratory

(Table 4 and 6), and in the 2009 Wheat Quality Council (WQC) testing program (see

attached Supplemental data). Direct comparison with TAM 111 indicates that it is similar

in test weight, seed size, hardness, and protein content. It has significantly stronger

mixing and baking strength compared to TAM 111 as measured by longer mix times,

longer stability, and larger loaf volumes. In the 2009 WQC trials, TX02A0252 was

7

compared to TAM 111 and had a significantly longer bake mix time, higher crumb grain

scores, finer crumb texture, improved crumb color scores, and larger loaf volume

(Attached Supplemental, page 3-7). The overall baking quality scores of TAM 111 and

TX02A0252 were 3.17 and 4.21, where 3 was average and 4 was good (Attached

Supplemental, page 7).

Summary

TX02A0252 is a medium maturing hard red winter wheat with excellent grain

yield potential; resistance to leaf, stripe, and stem rust; strong straw; and good hard red

winter wheat quality. It has excellent yield records in both irrigated and dryland locations

in the High Plains.

Grain yields have been similar to TAM 111 and TAM 112. In comparison to

TAM 111, it has better bread-making quality and better leaf rust resistance. In

comparison to TAM 112, it has better leaf rust and stripe rust resistance.

Release proposal for TX02A0252Data Table Contents

Compiled data from Texas AgriLife Research trials

Table 1. High Plains Irrigated data from 2007 through 2010.

Table 2. High Plains Dryland data from 2007 through 2010.Table 3. Leaf and Stripe Rust Scores from 2007 through 2010.

Table 4. Baking and mixograph data of grain harvested from the 2007 and 2009 Texas AgriLife Research High Plains Trials.

USDA Regional data

Table 5. Seedling leaf rust and stem rust scores of entries in the 2007 and 2008 Southern Regional Performance Nursery (SRPN).Table 6. Baking and mixograph data from 2007 and 2008 Southern Regional Performance Nursery (SRPN).

Supplemental data

60th Report of Wheat Quality, Hard Winter Wheat Technical Board of the Wheat Quality Council (selected pages)

Bushland

Etter

Bushland

Etter

Dalhart

Lubb

ock

Bushland

Etter

Perryton

Clovis

Bushland

Etter

Castro

Perryton

Clovis

TAM�W

�101

7160

2426

6378

4025

5377

7659

5251

91TA

M�111

9164

2528

7581

6238

5687

8860

6253

118

TAM�112

7868

3141

8479

4433

5187

8564

5958

105

TX02

A02

5210

065

2735

7565

6533

5876

8659

4554

96

Mean

8455

2431

7077

4929

5678

8060

5553

97CV

�(%)

5.8

11.6

10.1

13.9

9.8

9.4

10.2

7.5

10.8

8.4

4.7

7.2

11.6

8.3

4.6

LSD�(5

%)

7.9

10.4

4.0

7.0

11.2

10.1

8.21

3.57

9.8

10.6

6.2

7.1

10.3

7.1

7.2

entries�in�trial

4040

4040

4040

4040

4040

4040

4040

40

NAME

Grain

Yield

Test

Weight

Kernal

Weight

Flou

r�Protein�

14%mb

Hardn

ess�

Inde

xHeading

Height

Shatter

Lodging

bu/a

lb/bu

mg

%>50=hard

day�of�

year

cm%

%TA

M�W

�101

56.4

58.1

34.1

15.5

5912

7.3

710.9

1.0

TAM�111

65.9

58.8

30.9

14.8

5912

8.8

761.5

1.0

TAM�112

64.6

58.5

30.1

15.1

6512

4.6

742.0

0.0

TX02

A02

5262

.558

.329

.114

.763

129.2

732.4

1.0

locatio

n�years

1520

1212

126

132

3

High�Plains�Irrigated�data�from

�200

7�through�20

10

����������������������������������������Grain�Yield�(b

u/a)���������������������������������������

Table�1.�High�Plains�Irrigated�data�from

�200

7�through�20

10.�200

7�data�is�from

�the�TXE.�200

8�20

10�data�are�from

�the�UVT

�(com

plete�dataset�is�available�at�

<http://varietytestin

g.tamu.ed

u/whe

at/ind

ex.htm

>).

2007

2008

2009

2010

NAME

TX02

A02

52 P

ropo

sal T

able

sTa

ble

1. H

P-IR

R

Bushland

Etter

Claude

Sherman

Bushland

Etter

Claude

Canadian

Bushland

EtterClaude

Sherman

Perryton

Bushland

EtterGroom

Canadian

Herford

Perryton

Clovis

TAM�W

�101

4348

6458

1314

1062

1611

4516

4834

4242

4830

4855

TAM�111

5156

8453

1311

873

1919

5218

6136

5154

3934

5572

TAM�112

4756

8057

2112

1569

2021

5222

5740

4551

4037

5273

TX02

A02

5244

4892

7013

1513

7818

1855

2058

3645

4843

3451

73

Mean

4852

7455

1312

967

1616

4616

5436

4648

3931

4963

CV�(%

)8.2

14.2

8.7

17.3

14.6

26.9

21.4

7.1

9.8

18.3

6.0

16.7

7.1

5.9

9.9

11.8

19.7

18.2

4.2

5.4

LSD�(5

%)

6.4

12.1

10.4

15.3

3.0

5.1

3.1

7.8

2.6

4.9

4.5

4.5

6.2

3.5

7.4

9.1

12.5

9.2

3.4

5.5

n40

4040

4040

4040

4040

4040

4040

4040

4040

4040

40

NAME

Grain

Yield

Test

Weight

Kernal

Weight

Flou

r�Protein�

14%mb

Hardn

ess�

Inde

xHeading

Height

bu/a

lb/bu

mg

%>50=hard

day�of�

year

cm

TAM�W

�101

37.4

58.4

32.6

15.9

6012

6.2

60TA

M�111

43.0

58.4

28.2

15.5

6012

7.7

65TA

M�112

43.3

58.9

27.9

15.5

7012

4.9

64TX

02A02

5243

.659

.127

.415

.164

128.1

62

locatio

n�years

2023

1212

127

14

High�Plains�Dryland

�data�from

�200

7�through�20

10Across�Location

s�an

d�Ye

ars

Table�2.�High�Plains�Dryland

�data�from

�200

7�through�20

10.�200

7�data�is�from

�the�TXE.�200

8�20

10�data�are�from

�the�UVT

�(com

plete�dataset�is�available�at�

<http://varietytestin

g.tamu.ed

u/whe

at/ind

ex.htm

>).

2007

2008

2009

2010

�����������������������������������������������������������������������������Grain�Yield�(b

u/a)������������������������������������������������������������������

NAME

TX02

A02

52 P

ropo

sal T

able

sTa

ble

2. H

P-D

RY

2009

2010

2007

2009

Bushland

Castroville

Ellis

Luling

Castroville

McG

regorCastroville

Castroville

Bushland

Rossville,�KS†

Castroville

College�Statio

nTA

M�W

�101

20S

20MS

20MS

20MS

40S

10MR

30S

40SM

S70S

95S

20MS‡

50S

TAM�111

40S

30MS

10MR

20S

40S

15MR

40S

100S

tR2R

tRR

TAM�112

tStM

S�

�40

StR

30�80S

100S

80S

90MS

70S

60S

TX02

A02

52tS

tR5M

R5;

5MR�

20MS

tR5R

10R

10MR

15R

tRtR

Stripe

�Rust

2010

Table�3.�Leaf�a

nd�Stripe�Ru

st�Scores�from

�200

7�through�20

10.

Leaf�Rust

2007

2008

NAME

Fiel

d R

ust s

ores

: Sev

erity

(% in

fect

ed a

rea

on fl

ag le

af) a

nd re

actio

n in

th

e fie

ld a

t sof

t dou

gh s

tage

whe

re ‘S

’ = s

usce

ptib

le (l

arge

pus

tule

s w

ith

little

or n

o ch

loro

sis;

‘MS’

= m

oder

atel

y su

scep

tible

(med

ium

-siz

e pu

stul

es ty

pica

lly w

ith c

hlor

osis

; ‘M

R’ =

mod

erat

ely

resi

stan

t (sm

all

pust

ules

typi

cally

with

chl

oros

is o

r nec

rosi

s); '

R’ =

resi

stan

t (no

pus

tule

s or

min

ute

pust

ules

with

nec

rosi

s); a

nd ';

'= c

hlor

otic

hyp

erse

nsiti

ve

resp

onse

. t=

only

a tr

ace

was

obs

erve

d.

†Rossville,�KS:�inoculated

�(race�PST�100)�field�trial�con

ducted

�by�Dr.�Bob

�Bo

wde

n,�USD

A�ARS,�M

anhattan,�KS

TX02

A02

52 P

ropo

sal T

able

sTa

ble

3. L

R Y

R

Fiel

d R

ust s

ores

: Sev

erity

(% in

fect

ed a

rea

on fl

ag le

af) a

nd re

actio

n in

th

e fie

ld a

t sof

t dou

gh s

tage

whe

re ‘S

’ = s

usce

ptib

le (l

arge

pus

tule

s w

ith

little

or n

o ch

loro

sis;

‘MS’

= m

oder

atel

y su

scep

tible

(med

ium

-siz

e pu

stul

es ty

pica

lly w

ith c

hlor

osis

; ‘M

R’ =

mod

erat

ely

resi

stan

t (sm

all

pust

ules

typi

cally

with

chl

oros

is o

r nec

rosi

s); '

R’ =

resi

stan

t (no

pus

tule

s or

min

ute

pust

ules

with

nec

rosi

s); a

nd ';

'= c

hlor

otic

hyp

erse

nsiti

ve

resp

onse

. t=

only

a tr

ace

was

obs

erve

d.

†Rossville,�KS:�inoculated

�(race�PST�100)�field�trial�con

ducted

�by�Dr.�Bob

�Bo

wde

n,�USD

A�ARS,�M

anhattan,�KS

‡TAM�W

�101

�was�particularly�late�m

aturing�in�th

is�trial�and

�the�stripe

�rust�h

ad�

not�fully�develop

ed�at�the

�time�readings�were�taken.�

TX02

A02

52 P

ropo

sal T

able

sTa

ble

3. L

R Y

R

Pro

tein

14%

mb

Mill

ing

Yie

ldA

sh14

%m

bP

rote

in14

%m

b(%

)(%

)(%

)(%

)(%

)(m

in)

(%)

(min

)(c

c)

TX02

A02

5216

.072

.60.

4714

.868

.74.

634

66.9

6.63

1060

TAM

W-1

0116

.670

.60.

4316

.169

.83.

381

65.7

3.50

1100

TAM

110

14.8

70.1

0.46

14.4

66.9

3.88

464

.95.

7510

45TA

M 1

1116

.369

.00.

4315

.668

.93.

503

68.8

5.25

990

TAM

112

16.6

68.3

0.48

15.9

69.4

6.00

668

.18.

0011

95Ja

gger

17.2

71.6

0.49

16.3

68.1

3.50

267

.84.

8811

25

TX02

A02

5213

.570

.10.

4612

.261

.33.

133

61.3

4.50

920

TAM

110

13.7

66.9

0.38

12.2

62.8

3.25

362

.84.

0092

5TA

M 1

1113

.967

.80.

3712

.763

.53.

502

63.5

4.50

850

TAM

203

15.3

65.0

0.43

14.2

65.4

4.13

265

.44.

7595

0

TX02

A02

5213

.469

.80.

4211

.761

.43.

382

61.4

4.83

915

TAM

110

13.2

68.5

0.38

11.9

62.8

2.84

262

.83.

8390

5TA

M 1

1113

.169

.70.

3511

.763

.53.

032

63.5

4.12

820

TAM

203

15.9

65.3

0.45

14.6

66.5

3.50

166

.54.

0092

5

2009

Bus

hlan

d Irr

igat

ed

Wat

er A

bsor

p.M

ix ti

me

Loaf

Vol

ume

2007

Bus

hlan

d D

ryla

nd

2009

Bus

hlan

d D

ryla

nd

Tabl

e 4.

Bak

ing

and

mix

ogra

ph d

ata

of g

rain

har

vest

ed fr

om th

e 20

07 a

nd 2

009

Texa

s A

griL

ife R

esea

rch

Hig

h Pl

ains

Tria

ls. U

SD

A-A

RS

Har

d W

inte

r W

heat

Qua

lity

Labo

rato

ry, M

anha

ttan,

KS

.

Nam

e

Che

mic

alM

ixog

raph

Bak

ew

heat

flour

Wat

erA

bsor

p.P

eak

time

Tole

ranc

e

TX02

A02

52 P

ropo

sal T

able

sTa

ble

4. T

exas

Bak

e M

ixo

Pos

tula

ted

Pos

tula

ted

NA

ME

MC

RK

THB

JM

JBJ

TGB

GM

HD

SK

FBJ

TNR

JM

FPS

CM

LDS

BG

enes

QFC

SQ

THJ

RC

RS

R

KQ

QTP

MK

TTTT

TTK

SK

(Ug9

9)G

enes

St P

aul M

N

Fiel

dTX

02A

0252

0;;

3+;

0;33

+3+

3+;

Lr24

22/

S;1

/2-

22

;2;1

+Sr

24+

5RK

hark

of3+

3+3+

3+3+

3+3+

3+3+

-S

SS

SS

/;S

S-

40S

Sco

ut 6

63+

3+3+

;12-

;23+

3+3+

3+?

2+S

SS

SS

2+/S

-20

MR

-MS

TAM

107

3+3+

3+;/;

2/3+

33+

3+3+

3+Lr

14a

2/2+

22/

2++

2/;1

2/S

2/S

21A

.1R

TMR

Treg

o0;

;;1

-;

0;;1

-;

3+0;

Lr17

,Lr

242-

2;1

2/S

;2/S

2/S

224

5MR

Pos

tula

ted

Pos

tula

ted

NA

ME

MLD

STH

BJ

MJB

JM

FPS

TDB

JTD

BG

MH

DS

KFB

JG

enes

QFC

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24;1

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3+3

3+3+

-3/

2S

SS

SS

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10M

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ut 6

62+

33

3+3+

3+X

3+3+

Lr14

a2+

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SS

SS

-20

MS

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107

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+3+

3+3+

3+3+

3+-

2-2

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fect

ion

type

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mun

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o si

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ectio

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mal

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nded

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necr

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by

chlo

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=mod

erat

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with

out n

ecro

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or c

hlor

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; 4=l

arge

ure

dini

a w

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t nec

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s or

chl

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is; +

=ure

dini

a la

rger

than

nor

mal

; -=u

redi

nia

smal

ler t

han

norm

al. A

rang

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infe

ctio

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pes

is in

dica

ted

by m

ore

than

one

infe

ctio

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pe, w

ith th

e pr

edom

inan

t typ

e lis

ted

first

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ostu

late

d ge

nes:

+ =

not

abl

e to

iden

tify

Lr g

enes

with

race

s us

ed in

this

test

.

Tabl

e 5.

Seed

ling

leaf

rust

and

ste

m ru

st s

core

s of

ent

ries

in th

e 20

07 a

nd 2

008

Sou

ther

n R

egio

nal P

erfo

rman

ce N

urse

ry. T

ests

co

nduc

ted

by U

SD

A-A

RS

Cer

eal D

isea

se L

ab, S

t. P

aul,

MN

. Com

plet

e da

tase

t can

be

foun

d at

<h

ttp://

ww

w.a

rs.u

sda.

gov/

Res

earc

h/do

cs.h

tm?d

ocid

=119

32>.

2007

Ste

m ru

st

2008

Leaf

rust

isol

ates

isol

ates

Leaf

rust

isol

ates

Ste

m ru

stis

olat

es

TX02

A02

52 P

ropo

sal T

able

sTa

ble

5. S

RP

N s

eedl

ing

rust

Pro

tein

14%

mb

Mill

ing

Yie

ldA

sh14

%m

bP

rote

in14

%m

bP

rote

in14

%m

bM

illin

g Y

ield

Ash

14%

mb

Pro

tein

14%

mb

(%)

(%)

(%)

(%)

(%)

(min

)(%

)(m

in)

(cc)

(%)

(%)

(%)

(%)

(%)

(min

)(%

)(m

in)

(cc)

TX02

A02

5212

.473

.00.

4810

.961

.23.

174

61.0

4.58

855

12.1

70.1

0.40

10.7

60.8

3.71

462

.44.

8791

0K

hark

of16

.068

.70.

5414

.265

.42.

632

63.7

4.75

990

13.6

65.8

0.51

12.6

63.0

4.00

461

.85.

0095

5S

cout

66

14.7

73.2

0.45

13.2

64.8

2.63

263

.73.

6395

512

.770

.60.

4111

.562

.12.

713

62.0

3.18

890

TAM�107

12.3

71.5

0.43

11.2

60.7

2.62

461

.13.

6496

512

.369

.20.

3811

.061

.33.

103

62.1

4.09

900

Trego

12.4

71.7

0.42

10.9

61.0

2.49

158

.23.

0292

012

.069

.00.

3710

.659

.62.

492

59.9

3.32

840

TX02

A02

5214

.471

.60.

4913

.365

.23.

634

62.0

4.50

1040

14.5

69.4

0.45

12.8

64.2

3.13

259

.63.

2510

10K

hark

of17

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.30.

5315

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.93.

500

64.7

3.75

1000

15.2

64.9

0.41

13.5

63.4

4.13

361

.84.

7510

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cout

66

15.9

72.9

0.48

14.2

65.6

2.38

061

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0010

1014

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4213

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633

62.8

3.50

1010

TAM�107

14.2

71.0

0.44

13.1

63.7

3.13

360

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6310

1514

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4212

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.82.

630

59.1

2.75

960

Trego

14.7

71.9

0.45

13.2

64.9

3.38

160

.03.

5097

514

.069

.00.

4212

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380

59.0

2.75

915

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A02

5213

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4812

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251

59.8

3.50

950

14.4

70.6

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0010

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131

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13.7

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3.63

262

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14.9

72.4

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13.9

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162

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593

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4113

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251

63.5

3.50

925

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70.4

0.49

13.1

63.9

2.50

159

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5093

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4413

.563

.92.

380

62.9

3.63

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14.4

71.2

0.45

13.1

64.8

2.25

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3887

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4113

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130

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880

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posi

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f Sou

ther

n H

igh

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ns

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posi

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ther

n H

igh

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ater

Abs

orp.

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tim

eLo

afV

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posi

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f Sou

th C

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lain

s

Mix

time

Loaf

Vol

ume

whe

atflo

urW

ater

Abs

orp.

Pea

k tim

eW

ater

Abs

orp.

Tabl

e 6.

Bak

ing

and

mix

ogra

ph d

ata

from

200

7 an

d 20

08 S

outh

ern

Reg

iona

l Per

form

ance

Nur

sery

. US

DA

-AR

S H

ard

Win

ter W

heat

Qua

lity

Labo

rato

ry, M

anha

ttan,

KS

. Com

plet

e da

tase

t and

eva

luat

ion

prot

ocol

s ca

n be

foun

d at

<ht

tp://

hww

ql.g

mpr

c.ks

u.ed

u>.

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e

2007

2008

Che

mic

alM

ixog

raph

Bak

eC

hem

ical

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ogra

phB

ake

whe

atflo

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ater

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orp.

Pea

k tim

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lera

nce

TX02

A02

52 P

ropo

sal T

able

sTa

ble

6. S

RP

N B

ake

Mix

o

Texas-Amarillo: 2009 (Small-Scale) Samples a

as.d.= standard deviation; skcs = Single Kernel Characterization System 4100.

Test entry number 09-2417 09-2418 Sample identification TAM 111 (check) TX02A0252

Wheat Data FGIS classification 1 HRW 1 HRW Test weight (lb/bu)

Hectoliter weight (kg/hl)60.679.7

61.080.2

1000 kernel weight (gm) NIR hardness

26.764

25.577

Wheat kernel size (Rotap) Over 7 wire (%) Over 9 wire (%)

Through 9 wire (%)

56.742.80.5

33.364.72.0

Single kernel (skcs) Hardness (avg /s.d)

Weight (mg) (avg/s.d) Diameter (mm)(avg/s.d)

SKCS distribution Classification

73.3/15.8 26.8/7.7

2.51/0.30 01-02-15-82

Hard

80.2/17.0 28.6/8.8

2.61/0.30 01-02-10-87

Hard

Wheat moisture (%) Wheat protein (12% mb)

Wheat ash (12% mb)

9.614.41.44

9.613.81.53

Milling and Flour Quality Data Flour yield (%, str. grade)

Miag Multomat Mill Quadrumat Sr. Mill

69.767.4

69.568.1

NIR Flour moisture (%) NIR Flour protein (14% mb)

Flour ash (14% mb)

13.312.80.47

12.612.10.47

Glutomatic Wet gluten (%) Dry gluten (%) Gluten index

37.813.688.5

33.511.896.4

Rapid Visco-Analyser Peak time (min)

Peak viscosity (RVU) Breakdown (RVU)

Final viscosity at 13 min (RVU)

6.3222.378.8262.8

6.3203.466.2256.3

Minolta color meter L*a*b*

92.66-1.508.55

92.71-1.729.49

Falling number (sec) 416 453 Damaged Starch

(AI%) (AACC76-31)

95.625.98

96.616.76

158

Wheat Quality Council, 2009 harvest, TAM 111 vs TX02A0252.Selected pages from "60th Report of Wheat Quality, Hard Winter Wheat Technical Board of theWheat Quality Council".

<http://www.wheatqualitycouncil.org/>

TX02A0252 ProposalSupplemental Page 1

Texas-Amarillo: Physical Dough Tests and Gluten Analysis For 2009 (Small-Scale) Samples

Test Entry Number 09-2417 09-2418 Sample Identification TAM 111 (check) TX02A0252

MIXOGRAPHFlour Abs (% as-is) 68.1 66.2 Flour Abs (14% mb) 67.3 64.6

Mix Time (min) 3.38 3.88 Mix tolerance (0-6) 2 3

FARINOGRAPHFlour Abs (% as-is) 61.6 60.4 Flour Abs (14% mb) 60.8 58.8

Development time (min) 8.7 9.5 Mix stability (min) 31.0 41.0

Mix Tolerance Index (FU) 12 11 Breakdown time (min) 32.3 39.9

ALVEOGRAPHP(mm. H2O): Tenacity 76 93 L(mm): Extensibility 95 126

G(mm0.5): Swelling index 21.7 25.0 W(10-4 J): strength (curve area) 253 390

P/L: curve configuration ratio 0.80 0.74 Ie(P200/P): elasticity index 60.6 61.5

EXTENSIGRAPHResist (BU at 30/60/90 min) 327/481/515 424/582/670

Extensibility (mm at 30/60/90 min) 169/168/176 164/154/156 Energy (cm2 at 30/60/90 min) 105/159/186 129/174/194

Resist max (BU at 30/60/90 min) 466/734/851 600/951/999 Ratio (at 30/60/90 min) 1.9/2.9/2.9 2.6/3.8/4.3

PROTEIN ANALYSISHMW-GS Composition 2*, 7+9, 2+12 2*, 7+8, 5+10

Glu/Gli 2.52 2.33

HMW/LMW 0.34 0.34

%IPP 45.05 46.97

SEDIMENTATION TEST Volume (ml) 66.4 66.9

159

Wheat Quality Council, 2009 harvest, TAM 111 vs TX02A0252.Selected pages from "60th Report of Wheat Quality, Hard Winter Wheat Technical Board of theWheat Quality Council".

<http://www.wheatqualitycouncil.org/>

TX02A0252 ProposalSupplemental Page 2

0 1 2 3 4 5 6

Variety order by rank sum.

ncoop=chisq=

177.12

chisqc= 11.00cvchisq= 3.84

crdiff= 2.27Samples with the same letter not different at 5.0% level of significance.

0 1 2 3 4 5 6

Variety order by rank sum.

ncoop=chisq=

161.00

chisqc= 2.00cvchisq= 3.84

crdiff=No samples different at 5.0% level of significance.

mean=r sum=

3.6820.00

09-2417 TAM 111 (check)

09-2417 TAM 111 (check) mean=r sum=

3.9422.00

09-2418 TX02A0252 mean=r sum=

4.2826.00

mean=r sum=

4.4131.00

09-2418 TX02A0252

Cooperator Means

Cooperator Means

a

b

169

Wheat Quality Council, 2009 harvest, TAM 111 vs TX02A0252.Selected pages from "60th Report of Wheat Quality, Hard Winter Wheat Technical Board of theWheat Quality Council".

<http://www.wheatqualitycouncil.org/>

TX02A0252 ProposalSupplemental Page 3

0 1 2 3 4 5 6

Variety order by rank sum.

ncoop=chisq=

174.76

chisqc= 7.36cvchisq= 3.84

crdiff= 2.88Samples with the same letter not different at 5.0% level of significance.

mean=r sum=

3.0921.00

09-2417 TAM 111 (check)a

mean=r sum=

3.9930.00

09-2418 TX02A0252b

Cooperator Means

Frequency Table

09-2417TAM 111 (check)

09-2418TX02A0252

13 4 0

8 9 0

Open Fine Dense

172

Wheat Quality Council, 2009 harvest, TAM 111 vs TX02A0252.Selected pages from "60th Report of Wheat Quality, Hard Winter Wheat Technical Board of theWheat Quality Council".

<http://www.wheatqualitycouncil.org/>

TX02A0252 ProposalSupplemental Page 4

0 1 2 3 4 5 6

Variety order by rank sum.

ncoop=chisq=

178.47

chisqc= 9.60cvchisq= 3.84

crdiff= 2.71Samples with the same letter not different at 5.0% level of significance.

mean=r sum=

3.2619.50

09-2417 TAM 111 (check)a

mean=r sum=

4.1931.50

09-2418 TX02A0252b

Cooperator Means

Frequency Table

09-2417TAM 111 (check)

09-2418TX02A0252

6 10 1

3 7 7

Harsh Smooth Silky

174

Wheat Quality Council, 2009 harvest, TAM 111 vs TX02A0252.Selected pages from "60th Report of Wheat Quality, Hard Winter Wheat Technical Board of theWheat Quality Council".

<http://www.wheatqualitycouncil.org/>

TX02A0252 ProposalSupplemental Page 5

0 1 2 3 4 5 6

Variety order by rank sum.

ncoop=chisq=

177.12

chisqc= 8.64cvchisq= 3.84

crdiff= 2.80Samples with the same letter not different at 5.0% level of significance.

mean=r sum=

2.8720.00

09-2417 TAM 111 (check)a

mean=r sum=

3.8831.00

09-2418 TX02A0252b

Cooperator Means

09-2417TAM 111 (check)

09-2418TX02A0252

Frequency Table

1 1 4 6 5

0 0 0 4 10

GrayDark

Yellow Yellow Dull Creamy

0

3

White

0

0

BrightWhite

175

Wheat Quality Council, 2009 harvest, TAM 111 vs TX02A0252.Selected pages from "60th Report of Wheat Quality, Hard Winter Wheat Technical Board of theWheat Quality Council".

<http://www.wheatqualitycouncil.org/>

TX02A0252 ProposalSupplemental Page 6

0 1 2 3 4 5 6

Variety order by rank sum.

ncoop=chisq=

1711.53

chisqc= 14.00cvchisq= 3.84

crdiff= 1.81Samples with the same letter not different at 5.0% level of significance.

0 1 2 3 4 5 6

Variety order by rank sum.

ncoop=chisq=

179.94

chisqc= 9.94cvchisq= 3.84

crdiff= 3.07Samples with the same letter not different at 5.0% level of significance.

mean=r sum=

2.7518.50

09-2417 TAM 111 (check)

09-2417 TAM 111 (check)a mean=r sum=

3.1719.00

09-2418 TX02A0252b mean=r sum=

4.2132.00

mean=r sum=

4.5032.50

09-2418 TX02A0252

Cooperator Means

Cooperator Means

a

b

178

Wheat Quality Council, 2009 harvest, TAM 111 vs TX02A0252.Selected pages from "60th Report of Wheat Quality, Hard Winter Wheat Technical Board of theWheat Quality Council".

<http://www.wheatqualitycouncil.org/>

TX02A0252 ProposalSupplemental Page 7