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1 Proposal for the Release of “Rampur Hybrid-2Developed from RML-4 and NML-2 Submitted by National Maize Research Program (NMRP), Rampur Proposed by DB Gurung § , SR Upadhyay § , TR Rijal § , CB Kunwar § , BR Pandey § , JB Kshetri § , DC Paudel § , BH Adhikary § , G KC § , P Thakur § , IP Upadhyay § , BB Pokharel § , J Shrestha § , B Bhandari § , N Dhakal § , GS Bhandari § , KP Dhital § , RK Rajak £ , YP Sah , CL Chaudahry , S Poudel , N Dhami , KP Bhurer £ and MP Tripathi § A Joint Accomplishment of: § National Maize Research Program (NMRP), Rampur £ Regional Agriculture Research Station (RARS), Parwanipur Regional Agriculture Research Station (RARS), Nepalgunj Agriculture Research Station (ARS), Belachapi Regional Agriculture Research Station (RARS), Tarahara Agriculture Botany Division (ABD), Khumaltar July, 2011 Draft copy

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Page 1: Proposal for the Release of Hybrid Maize Variety - 2011

1

Proposal for the Release of “Rampur Hybrid-2” Developed

from RML-4 and NML-2

Submitted by

National Maize Research Program (NMRP), Rampur

Proposed by

DB Gurung

§, SR Upadhyay

§, TR Rijal

§, CB Kunwar

§, BR Pandey

§, JB Kshetri

§, DC

Paudel§, BH Adhikary

§, G KC

§, P Thakur

§, IP Upadhyay

§, BB Pokharel

§,

J Shrestha§, B Bhandari

§, N Dhakal

§, GS Bhandari

§, KP Dhital

§, RK Rajak

£,

YP Sah₫, CL Chaudahry

€, S Poudel

†, N Dhami

‡, KP Bhurer

£ and MP Tripathi

§

A Joint Accomplishment of:

§National Maize Research Program (NMRP), Rampur

£Regional Agriculture Research Station (RARS), Parwanipur

€Regional Agriculture Research Station (RARS), Nepalgunj

₫ Agriculture Research Station (ARS), Belachapi

†Regional Agriculture Research Station (RARS), Tarahara

‡Agriculture Botany Division (ABD), Khumaltar

July, 2011

Draft copy

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Application to be made for release of new maize varieties

Government of Nepal

Ministry of Agriculture and Cooperatives

National Seed Board

Format for submission of proposal for the release of “Rampur Hybrid-2” maize

variety

The Variety Approval, Release and Registration Sub-committee (VARRC)

National Seed Board Secretariat

Seed Quality Control Centre

Harihar Bhawan, Lalitpur

Tel. +977-01-5521359

Sub: Application for the release of maize variety “Rampur Hybrid-2”

As, we have bred the seeds of new variety described below, we hereby make this application for

the release of the seeds of the variety.

Original entry name Suggested name

English Nepali

RML4/NML2 Rampur Hybrid 2 Rampur Barnashankar -2

RML4 (CA00326) NML3

NML2 ( CML-430) NML2

1. Background information

In Nepal, maize is the second most important crop after rice in terms of area and production. It

occupies a momentous position because of the multiple uses of this crop. It can be used as food,

feed, fodder, fuel and raw materials for various industrial purposes. Maize is the main ingredient

for poultry and animal feeds. The exponential growth of the human population and change of

food habit in the recent years, people are consuming more milk, meat, fish and eggs because of

increasing trends of income every year. The increased demand of those items needs more maize

grains for poultry and animals feeds. Maize has low reserve in the international market compared

to other cereal crops and has not a market problem worldwide because of the high demand of

milk and meat products. Farmers in maize growing countries including Nepal have not problem

of marketing of maize grains. In developed countries, maize is considered as an industrial crop

whereas in Nepal, it is emerging as cash crop in the areas where green cobs are sold. In addition,

Maize grain produced in many terai and in some mid hill districts can sell their maize

immediately after harvesting and thus emerging maize as cash crop. The ever increasing demand

of maize cannot be met easily simply cultivating with open pollinated improved varieties. The

productivity of OPVs cannot be increased from a certain level even if provided with more

fertilizers and improved crop management. The productivity of maize can be increased by

growing with different types of hybrids particularly single cross hybrids. In the recent years,

there is overwhelming demand of hybrid seeds and hybrid technology mostly in terai and inner

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terai where hybrids are grown after rice in winter and spring seasons. The demand is also there in

some mid hill districts and in valleys of foot hills and river basin areas.

Of the 23 maize cultivars released in Nepal, Gaurav, single cross hybrid maize, which was

released in 2003 in the history of hybrid maize breeding efforts initiated since 1987. This

historical hybrid could not be popularized in hybrid maize growing areas because of the

difficulty in its seed production particularly in private sector. This situation has left no varietal

options for growing hybrid maize in Nepal and farmers have to rely on seeds of multi-national

hybrid companies based mostly in India. In many cases, farmers are paying higher prices for

hybrid seeds and in some cases; they are also cheated both in price and quality. The available

options for the hybrid maize growing farmers are limited. Either they should grow improved

OPVs developed by NMRP or go through the Indian hybrids sold by private seed entrepreneurs.

They grow Indian hybrids simply because of unavailability of seeds of our Nepalese hybrids,

higher yields of hybrids compared to OPVs, uniformity in maturity, and tolerance to biotic and

abiotic stresses.

To address this real situation, development of high yielding as well as competitive hybrids and

releasing hybrids in a regular basis is the sustainable way of increasing production and

productivity of maize in order to meet the ever increasing demand of maize for feeds in

accessible areas and human consumption for mid hills. Releasing hybrid varieties in a cyclic

order will create more varietal options and make breakthrough in the history of maize production

in Nepal with hybrids and hybrid technology, which ultimately help to improve food security and

reduce poverty and hunger in the country. Keeping all these in consideration, NMRP has thus

prepared and submitted a proposal to the National Seed Board (NSB) for release a single cross

maize hybrid with proposed name is Rampur hybrid-2 in English and Rampur Barnashankar -2

in Nepali.

2. General Information

2.1. Common name: Maize

2.2. Botanical name: Zea mays L

2.3. Original designation/Symbolic name used in testing: RML4/NML2

2.4. Pedigree: RML4 (CA00326)/NML2 (CML430)

2.5. Cross (parents): RML4/NML2

2.6. Country of Origin: Nepal

2.7. Source of material (farmer/community, name of the experiment and the year in which

it was first introduced): CIMMYT

2.8. Years and location of testing of the variety in Nepal

Table 1. Summary of the trials conducted through NMRP, Rampur in 2005-2011 Year Experiments Locations References1

2005 Inbred line Observation nursery Khumaltar Annexure 1

2005 Diallel crosses Rampur, Parwanipur Annexure 2- 3

2006/07 Observation nursery Rampur Annexure 4- 5

2010W Agro-morphological characterization Rampur Annexure 6

2006/07 CVT Rampur Annexure 7

2007W CVT Rampur Annexure 8

2007W CVT Rampur Annexure 9-10

2010-11W Multi-location testing of hybrids Rampur, Parwanipur, Tarahara Annexure 12

1 Please, find the details of experimental results referred as Annexure numbers at the end.

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2009 Front line demonstration Rampur Annexure 11

2007 Agronomic trials Rampur Annexure 20

2008W Agronomic trials Rampur Annexure 21-25

2009W Agronomic trials Rampur Annexure 26-28

2007W CFFT Rampur Annexure 17

2008 CFFT Rampur, Parwanipur, Belachapi Annexure 18

2007w CFFT Rampur Annexure 19

2010S Heat stress screening nursery Parwanipur, Rampur, Nepalganj Annexure 13-16

2006S Disease screening nursery Rampur Annexure 29

2009S Disease screening nursery Rampur Annexure 30

2009 Insect screening nursery Rampur Annexure 31-32

2.9. Farmers Field Trial (FFT), participatory trial data/information and stakeholders’ preferences

Days to 50% silking ranged from 71 in early winter to 119 days in late winter in the farmers field

condition (Table 2). The yield performance of this genotype is highest (8183 kg/ha) when

planted in early winter.

Table 2. Performance of RML4/NML2 under farmers condition (Annexure 17-19) Year Experiments Location Days to

50%

silking

Plant

height

(cm)

Ear

height

(cm)

Grain

yield

(kg/ha)

2007EW CFFT Rampur 71 210 110 8183

2008LW CFFT Rampur, Parwanipur,

Belachapi

111 133 52 5677

2007LW CFFT Pithuwa 119 170 95 5701 EW=Early winter, and LW= Late winter

2.10. Others, if any:

Figure 1: Joint regression analysis of grain yield (kg ha

-1) of RML4/NML2 maize

hybrid over standatrd check (n=16)

Rampur Composite is well known for its grain yield stability over the location and years among

the maize growers. Probably, it is the most adaptive maize variety ever released by the National

Maize Research Program (NMRP). Joint regression analysis of the proposed hybrids with

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Rampur Composite (Figure 1 and Table 3) showed that RML4/NML2 is more stable (b=0.91 i.e,

b= nearly unity) and mean grain yield 36.7% higher than standard check (Rampur Composite).

Table 3. Joint regression analysis parameters of maize hybrids and Rampur Composite Genotypes Mean±SEm Range b SEb T value

RML4/NML2 6393±530 2308-9255 0.9124 0.2369* 3.85

Rampur Composite 4675±266 3262-6307 0.4810 0.1122* 4.29

3. Summary of Varietal Characteristics

3.1 Agronomic

This is a full-season hybrid and has yield potential up to 10.5 tones/ha. Kernel is yellow and flint

type. Plant stature is similar to Gaurav hybrid. Its erect leaves and medium plant height offers

high density planting. Stem is robust and has no lodging problem. This hybrid can be grown in

the winter, spring and summer seasons in terai and inner terai regions. However, main growing

seasons are winter and summer seasons. It performs well in winter as compared to spring and

summer seasons.The difference between days to tasseling and silking is 3-5 days but tasseling

days ranged from 64 days in summer to 103 days in late winter. Silking days ranged from 67 in

summer to 107 days in late winter (Table 4 and Annexure 3-15). The hybrid produced higher

grain yield in early winter planting (7005 kg ha-1

) followed by late winter (5579 kg ha-1

) and

summer (3555 kg ha-1

). The major morphological traits of the inbred lines are presented in the

Table 5.

Table 4. Major agro-morphological traits of RML4/NML2 maize hybrids (Annexure 4-16) Parameters Winter Summer

Early Late

Days to 50% anthesis 66 (63-75)§ 103 (91-119) 64 (58-68)

Days to 50% silking 71 (68-78) 107 (95-122) 67 (59-71)

Plant height (cm) 203 (153-225) 126 (95-176) 166 (153-181)

Ear height (cm) 113 (72-137) 53 (37-80) 78 (68-91)

Grain yield (kg ha-1) 7005 (5224-8694) 5579 (4395-7188) 3555 (2308-4802) §Figures in the parenthesis shows the range

Varietal characteristics of parental lines (Inbred lines)

Seed parent (RML-4) is short and has cylindrical ears with irregular row arrangement and

produces a good seed yield 1.5- 3 t ha-1

. Pollen parent (NML-2) has medium plant height with

erect leaves and glabrous leaf sheath. Pollen parent takes 69 GDU to shed pollen of seed parent

when the seeds are planted at the same time. Parents are easy for crossing and varietal

maintenance is not so difficult.

Table 5. Agro-morphological traits of RML4 and NML2 inbred lines (Source: Annexure 6)

Parameters RML4 NML2

Days to 50% Tasseling 59 71

Days to 50% silking 62 75

Plant height (cm) 120 190

Ear height (cm) 60 80

Grain yield (kg/ha) 1250 1500

GDU for Tasseling 953 1036

GDU for Silking 967 1050

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Fig 2. RML-4 x NML-2 in field at

NMRP, Rampur

Fig 3. Ears of RML-4 x NML-2

Fig 4. Seed Parent (RML-4) Fig 5. Pollen parent (NML-2) Fig 6. Ears of RML-4

3.2. Response to stresses (please specify):

3.2.1 Biotic stresses

(a) Insects: Among the 32 tested genotypes including OPVs, QPM, Hybrids and Local

check in Rampur. It is apparent from the results that RML4/NML2 showed minimum

infestation of maize stem borer. The exits holes formed by the pests were also found

minimum in this genotype (Annexure 31).

(b) Diseases: Hybrid RML-4/NML-2 has shown resistant to moderately resistant reaction

against leaf blight diseases (Northern leaf blight, Southern leaf blight and Banded leaf

and sheath blight (BLSB) in natural condition (Annexure 29 and 30), though it’s

inbred exhibited moderately susceptible reaction against NLB under artificially

inoculated condition (Annexure 4 and 30).

(c) Weeds: No specific association of weeds with this variety and does not require any special

care for weed management. However, the field should be free from weed to attain

higher grain yield and crop management practices applied to other maize hybrids or

OPVs is also sufficient for this variety.

3.2.2 Abiotic stresses

(a) Adverse climatic conditions: The hybrid maize lines along with other promising lines

were evaluated under heat screening nursery at NMRP, Rampur; RARS, Parwanipur;

and RARS, Tarahara in summer season of 2010 (Table ). The results showed that it

prefer favorable environment rather than heat stress condition for higher yield

performance. The crop could not perform well under any type of stress condition such

as water stagnation, drought, heat and cold stresses. The genotypes can flourish its

potentiality under optimum condition.

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(b) Adverse soil conditions: The crop prefers loam to sandy loam soil for better yield

performance. It requires frequent irrigation and heavy dose of manure and fertilizer

than OPVs to obtain expected yield.

(c ) Other stresses, if any:

3.3. Grain/leaf/root/curd/bulb/seed/fruit/tuber qualities:

3.3.1 Nutritional quality (if available):

Table 6. Nutritional analysis of RML4/NML2

Traits Value

Ash (%) 1.29

Moisture (%) 14.73

Fat (%) 3.06

Protein (%) 7.59

Crude fiber (%) 2.02

Carbohydrates (%) 71.31

Thousand grains weight (gm) 287

Bulk density (gm/litre) 816

Source: Food Research Unit, 2011

3.3.2 Processing quality: 69% grit, 24.1% flour, 6.9% bran and 4:1 grit:flour ratio

3.3.3 Organoleptic test (cooking quality, taste, aroma/flavour, etc): % popping=15.5%

(machine), 60% (earthen pot), 45% (metal pot)

3.3.4 Other specific qualities (if any): Stay Green characters

3.4. Other characteristics (threshing, storability, market potential etc.):

4. Morphological characteristics (size, shape, colour, etc of various plant parts):

Table 7. Major morphological traits of proposed hybrids and its inbred lines Traits RML-4/NML-2 RML4 NML 2

Stem color Green Green Green

Plant height Medium Medium Tall

Leaf color Green Green Green

mid rib color White White White

Leaf sheath Green Green Green

Leaf orientation semi erect Semi erect Semi erect

No. of leaves below ear 8

No. of leaves above ear 5

Tassel type Semi Open Semi Open Semi Open

Tassel color purple white white

Tassel branch 11-12

Silk color pink white Semi purple

Grain color Yellow Yellow Yellow

Grain type Semi flint Semi dent Flint

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4.1. Major identifying characteristics of a crop variety for cultivar authenticity:

The genotype can be distinguished from others maize genotypes by following traits.

RML4/NML2: Kernel is yellow and flint type. Erect and stay green leaves, robust stem

and medium plant height.

RML4: Seed parent is short plant height, cylindrical ears with irregular row arrangement

NML2: Pollen parent has medium plant height with erect leaves and glabrous leaf sheath

4.2. Molecular characteristics (Not available)

5. Recommendation domain:

5.1 Geographical area (altitude, latitude and longitude): The hybrid was tested at many locations

from east to mid-western terai in the various experimental trials i.e. screening nursery, observation

nursery, CVT and FFT (Table 1). Therefore, this variety is proposed to recommend for commercial cultivation from east terai to mid-western Terai, and inner Terai of Nepal.

5.2 Moisture regime: partially irrigated and irrigated. Soil moisture as well atmospheric

moisture is critical at 7 days before and after flowering.

5.3 Climatic conditions: If the atmospheric temperature rises more than 38oc pollen grains start

to die by appearing tassel firing symptoms and atmospheric temperature below 8o C is not

also suitable during flowering time.

5.4 Cropping pattern:

The variety is suitable under both maize and rice based cropping system. However, higher

level of production can be obtained when the crop is grown in winter rather than summer

planting.

a. Maize based cropping system:

Maize-Soybean-Vegetables

Maize-Rajma-Vegetables

Maize-Sesame-Maize

Maize-Black gram-tori etc.

b. Rice based cropping system:

Rice-Maize-Mungbean

Rice-Maize-Cowpea

Rice-Maize-Vegetables

Rice-Wheat-Maize

Maize-Maize-Rice etc.

5.5 Socioeconomic conditions: The farmers who are growing maize in terai and inner terai

condition can easily grow this variety. However, this variety requires special care and

attention than OPVs but similar level of management as given to Indian hybrids is required.

Large scale seed production of inbred lines and hybrid seed production can be done by

private sectors with qualified manpower and by an enthusiastic people who can bear the risk

of handling large scale seed production and can get benefits from this technology. Since the

variety has stay green character, farmers can use as fodder after harvesting and get more

benefits from this variety than others from milk and milk products. So, the variety is friendly

even to resource poor farmers, who can get higher yield per unit area.

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5.6 Economic analysis and benefit: Hybrid seed production could be a very remunerative seed

business in Nepal. Modern inbred lines give at least 3 t ha-1

of seeds. Seed parent covers

about 75% of total area if we plant in a ratio of 3:1. If we assume only 80% pollination

success, hybrid seed yield from 1 ha would be 3000 x 0.8 x 0.75 = 1800 kg of seeds. Retail

price of different categories of hybrids in market is ranged from NRs 85- 500/kg. If we sell at

whole sale rate of NRs. 150/kg, 1 ha will generate a total income of 1800 x 150 = NRs. 270,

000. Cost involved in seed production depends on the localities where the seed production is

done. Based on the rough estimation, Rs.100, 000/ha would be sufficient to cover all the

production costs. Thus, net profit would be Rs. 170,000 per ha. This clearly shows that

hybrid maize seed production is a highly profitable business. Private seed companies should

come forward to commercialize the hybrid seed production as their role is crucial in

commercialization of hybrid seed and hybrid technology.

6 Production and management aspect (Please add detailed information, if necessary, on the

general cultivation practices; appropriate input and moisture regimes): Package of practice is

similar to the Gaurav, released in 2003 and other hybrids of multi-national companies, which

are growing by farmers in hybrid maize growing districts.

6.1 Agronomic Practices:

2-3 ploughings are needed for land preparation. First ploughing should be carried out 30

days prior to maize sowing.

The appropriate date of maize sowing in Terai and inner Terai of Nepal ranges from 15th

August to15th October, respectively. However, it can be grown before 15

th November.

The recommended dose of fertilizer 160:60:40 N:P2O5:K2O kg ha-1

along with 10-15 tons

of farm yard manure. However, it gives highest grain yield upto 180 K2O kg ha-1

nitrogen

(Annexure 28). FYM and full dose of P205 and K20 along with 50% nitrogen should be

applied during last ploughing (before crop sowing). 25% Urea should be top-dressed

during knee high stage and 25% just prior to tasseling and silking.

Seed rate 25 kg ha-1

(66666 plants/ha) for general grain production.

The recommended planting space for hybrid variety is given in (Annexure 21, Annexure

23 and Annexure 24):

Row-to-Row distance: 75 cm

Plant to plant distance: 20 cm

6.1.1 Weeding: First weeding should be done within 20-25 days and second weeding (earthing

up) within 40-45 days after the germination. Priority should be given to manual weeding.

However, Glyphosate @ 5ml/litre as pre-planting and Pendimethylene @ 5.5 ml/litre can be

applied as pre-emergence. Post emergence can be applied with 2-4-D @ 3ml/litre of water.

6.1.2 Irrigation: Knee high stage, tasseling and silking, and grain filling stages are most critical

for moisture stress. Light irrigation should be applied to the maize crop at these stages depending

on the soil moisture status and irrigation facilities.

6.1.3 Detasseling: If weather condition is normal (high humidity and temperature less than 38oc

),

30% plants can be detasseled, which helps to increase maize production up to 7%.

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6.1.4 Harvesting: Crop should be harvested after physiological maturity. Maize can be

harvested when black layer is formed on maize grains.

6.1.5 Drying: Maize ears should be sun dried for 3-4 days and before shelling. Grains should be

dried on the tarpaulin and covered during the night. When the grain moisture is reduced down

to 12%, then grains are either stored in metal bins or kept in sealed bags after processing of

seeds.

7. Reasons for release

There are very limited hybrid varietal options for growing hybrids in Nepal. A single

cross hybrid “Gaurav “ was released in 2003 but it could not be popularized among the

farmers due to difficulty in its seed production particularly in private sector. NMRP could

not release hybrids in a regular basis due to low investments of resources in hybrid

breeding program. Due to unavailability of hybrid seeds within the country, farmers

mostly in terai, inner terai and in road accessible areas of foot hills started to buy Indian

multi- national hybrid seeds due to high yield potentiality and uniformity of hybrids over

OPVs. However, farmers in those areas had been growing maize hybrids since many

years. Nowadays, there is overwhelming demand of hybrid seeds due to high demand of

maize grains for poultry, animal feeds and other industrial purposes.

Farmers in hybrid maize growing areas in Terai and in some mid hill districts are using

hybrid seeds mostly from multi-national seed companies based mostly in India and seeds

are expensive ranging from NRs, 85 to 500/kg. Huge amount of money is spent to

purchase maize seeds from India. This investment can be minimized and outgoing money

can be minimized and saved. Seeds will be cheaper as we produced within the country

and quality is assured and there is no chance cheating farmers in the name of fake hybrid

seeds.

Releasing this variety creates a better opportunity for commercialization in hybrid maize

seed production in private sector and could benefit the farmers, seed growers and others,

who are involved in seed production and distribution chains. Country will be benefit in

the long run with improved food security through increased production.

This variety is moderately resistant to resistant against foliar diseases especially Northern

leaf blight, Southern leaf blight, and Banded leaf and sheath blight. It gives stable and

higher grain yields up to 8.6 t ha-1, uniformity in plant, ear height and in maturity.

As it creates more varietal options and farmers will have choices for growing maize

hybrids. Dependency in importing maize grains required for poultry and animal industries

will be reduced and steps towards self-sufficiency of maize in the country will be kicked

off.

8. Seed supply situation NMRP has been maintaining numbers of inbred lines and many released and pre –released maize

varieties since many years. Although the available seed of these inbred lines and hybrids are not

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sufficient for selling in the market but it can provide sufficient amount of source seed for seed

multiplication purpose as demanded by the interested stakeholders.

Table 8. Seed supply situation of inbred lines and hybrids at NMRP, Rampur SN Category of seed Amount available (kg) Where available Person handling

1 RML4 5 NMRP, Rampur B Bhandari

2 NML2 3 NMRP, Rampur B Bhandari

3 RML4/NML2 20 NMRP, Rampur B Bhandari

9. Acknowledgements

The present work is an outcome of a team and collaborative work in varietal development, which

is a part of regular research activities of National Maize Research Program (NMRP), Rampur.

We are deeply indebted to the NMRP staffs and collaborators, farmers and others who are

directly or indirectly involved in the development and evaluation phases of this hybrid. We are

thankful to CIMMYT for providing inbred lines during the initial stage and other NARC

collaborating stations in providing support in evaluation process. Hybrid maize breeding team is

grateful to the NARC management for over all support in hybrid maize breeding program.

10. References NMRP, 2007. Proceedings of the 25

th National Summer Crops Research Workshop on Maize Research

and Production in Nepal, June 21-23.

NMRP, 2008. Annual Report 2063/64 (2006/07). National Maize Research Program (NMRP), Rampur

NMRP, 2009. Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur, Chitwan, Nepal

NMRP, 2010. Annual Report 2065/66 (2008/09). National Maize Research Program (NMRP), Rampur,

Chitwan, Nepal

NMRP, 2011. Annual Report 2066/67(2009/10). National Maize Research Program (NMRP), Rampur

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Proposed by

Signature:

Name: Dr. Dil Bahadur Gurung

Main proposing organization: National Maize Research Program (NMRP), Rampur

Designation: National Maize Coordinator /Plant Breeder

Corresponding address: National Maize Research Program (NMRP), Rampur,

Chitwan

Tel. +977-56-591001

Fax: +977-56-592548

Mobile: 9851074849

Email: [email protected]

Website if applicable:

Page 13: Proposal for the Release of Hybrid Maize Variety - 2011

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Annexes

Annexure 1: Inbred lines Observation Nursery, Khumaltar in 2005 SN Inbred lines Plant

height

(cm)

Ear

height

(cm)

Turcicum

Leaf Blight

(TLB)

Husk

cover

Ear

Aspect

Grain yield

(kg ha-1)

1 RML-59 177 95 2 3.5 1 6840

2 RML-37 176 81 2 1.2 1.3 4869 3 RML-17 194 102 1.5 1 2 4213

4 RML-54 211 106 2 1.8 1.5 3795

5 RL-57 174 77 1.5 1 1.8 3779

6 RML-3 208 118 1.5 1 1.5 3515

7 RL-41 164 74 2 1 1.3 3453

8 RL-31 180 98 2 1 1.6 3109

9 RML-8 142 67 2 1.2 2.8 2573

10 RML-5 170 76 2 1 1.2 2504

11 RL-35 160 66 1.5 1 2 2389

12 RML-18 155 61 1.5 1 2.8 2235

13 NML-1 190 90 1 1.2 2.2 1867

14 RML-24 178 79 2 1 1.6 1840 15 RL-1 160 81 2.5 1.2 2.8 1699

16 RML-51 135 67 1.5 1 1.5 1491

17 RML-4 129 62 2.5 1.2 2.5 1427

18 RML-57 137 71 1.5 1 3 1360

19 NML-2 182 101 2 1 2.5 1203

20 RL-30 157 66 1.5 1 2.8 1173

21 RML-6 140 73 2.5 1.2 2 1115

Mean 168 81 1.8 1.2 2 2688

Annexure 2: Mean grain yield (kg ha-1

) over locations of F1 hybrids Parents NML2 RML3 RML4 RML5 RML6 RML7 RML8 xi. Mean

NML1 7277 8693 7240 9673 7136 5361 7570 52950 7564 NML2 6705 7861 8728 9058 6786 6848 53263 7609

RML3 8409 7529 8230 7772 8646 55984 7998

RML4 7901 6326 8519 7698 53954 7708

RML5 7625 7465 8139 57060 8151

RML6 8309 8011 54695 7814

RML7 7401 51613 7373

RML8 54313 7759

Grand mean 7747

Source: Proceedings of the 25th National Summer Crops Research Workshop on Maize Research and

Production in Nepal, June 21-23, 2007

Annexure 3: Mean grain yield of 28 Diallel crosses (Hybrids) from 8 maize inbred lines and checks at Rampur and Parwanipur in winter season of 2005

SN Cross Mean grain yield (kg ha-1)

Rampur Parwanipur Combined

1 NML2/NML1 6719 7835 7277 2 RML3/NML1 8582 8803 8693 3 NML1/RML4 7557 6923 7240 4 RML5/NML1 9321 10025 9673 5 RML6/NML1 7939 6334 7136 6 RML7/NML1 5357 5366 5361 7 RML8/NML1 7351 7789 7570 8 NML2/RML3 5577 7832 6705

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9 RML4/NML2 7890 7831 7861 10 NML2/RML5 8614 8842 8728 11 NML2/RML6 9327 8789 9058 12 NML2/RML7 7197 6376 6786 13 NML2/RML8 6633 7064 6848 14 RML4/CRML3 8792 8025 8409 15 RML30/RML5 7952 7105 7529 16 RML3/RML6 8306 8154 8230 17 RML3/RML7 6476 9068 7772 18 RML3/RML8 8914 8378 8646 19 RML4/RML5 7855 7946 7901 20 RML4/RML6 5239 7413 6326 21 RML4/RML7 9118 7919 8519 22 RML4/RML8 8812 6584 7698 23 RML5/RML6 7839 7411 7625 24 RML5/RML7 6942 7988 7465 25 RML5/RML8 8317 7961 8139 26 RML6/RML7 9392 7226 8309 27 RML6/RML8 8200 7822 8011 28 RML7/RML8 6915 7886 7401 29 JKMH1001 5824 6604 6214 30 GAURAV 6235 9530 7883 Grand mean 7640 7761 7700 Location (L) NS Genotypes (G) ** L x G NS CV, % 16.6 SE (Loc) 281.1 SE (G) 639 SE (L x G) 904 Source: Proceedings of the 25th National Summer Crops Research Workshop on Maize Research and Production in

Nepal, June 21-23, 2007

Annexure 4: Mean grain yield and other yield attributing characters of maize hybrids in observation

nursery at NMRP, Rampur in 2006/07 Cross 50 %

anthesis

50 %

silking

Plant

height

(cm)

Ear

height

(cm)

Grain

yield

(kg ha-1)

Plant

aspect

(1-5)

Ear

aspect

(1-5)

Helminthosporium

leaf blight (HLB)

RML4/RML3 89 94 118 67 9077 1.5 1.5 8.7

RML73/RML72 104 106 143 58 8189 1 1.5 18.3

NML2/RL12 100 107 127 58 7848 1.5 2.5 11.7

NML1/RL17 93 102 135 62 7592 2 1.5 18.3

RML52/RML51 93 100 137 70 7087 1.5 2 12.3

RL17/P145-16-1-BBBB 93 97 118 53 6912 2 3 26.7

RC/RL189 83 86 143 68 6805 2 2 10

RL30/RML6 93 97 117 48 6701 1.5 1.5 15

RML3/NML2 94 102 133 68 6588 1.5 2 9.3

RML54/RL51 86 93 140 57 6586 1.5 1.5 15

RL14/RL35 102 104 118 53 6578 1.5 3 14.3 GAURAV 99 104 128 58 6508 1.5 2 23.3

RML5/NML2 99 104 127 53 6483 1.5 2.5 25

RML51/RML53 85 93 140 68 6461 2.5 2 18.3

RL17/ARUN2 85 87 137 57 6453 1.5 1.5 25

RML17/ARUN2 91 93 167 83 6393 2 1.5 28.3

RC/RML4 86 88 105 48 6367 2 2 31.7

RC/RML17 90 93 140 58 6334 1.5 2.5 13.3

NML2/RL17 105 104 122 53 6324 1.5 2 15

RC/NML1 84 86 130 60 6322 2 2 25

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RML4/ARUN2 85 88 117 47 6049 2 1 38.3

RC/RML6 83 87 155 60 6014 1.5 2 16.7

RML7/RML8 86 97 138 63 5906 1 2 26.7

RL49/RL85 81 81 157 70 5849 1.5 2 43.3

NML1/ARUN2 84 85 117 50 5801 2 1 23.3

PIONEER30G10 86 88 128 48 5725 1.5 2 23.3

RML4/NML2 99 101 132 65 5672 1.5 1.5 18.3 RC/NML3 76 82 140 65 5646 1.5 2.5 13.3

RL30/RL35 89 91 117 52 5611 1.5 2 26.7

RML6/NML2 96 98 138 55 5579 1.5 1.5 13.3

RC/NML18 83 87 148 62 5466 2 2 31.7

RL7/RL90 87 91 115 45 5454 1.5 2 48.3

RL19/RL177 81 85 173 80 5383 2 2 33.3

RC/RML5 77 82 138 67 5260 1 2 18.3

RML18/RML17 99 98 110 38 5192 1.5 2 25

RL7/RL94 86 93 117 52 5161 2 2 43.3

RL19/RL161 83 85 150 62 5147 1.5 3 26.7

RL19/RL166 85 87 165 78 5085 2 2.5 25

NML1/RML7 89 97 108 43 5014 2 2 31.7 RL14/RML8 88 97 127 53 4894 1 2.5 11

RML18/ARUN2 81 84 128 53 4869 2.5 1.5 38.3

RL14/MUSTANG 66 68 110 37 4852 2 2 36.7

RC/RML7 84 79 138 67 4823 2 3 43.3

RL21/RL35 100 105 108 52 4798 2.5 3 35

RL19/RL167 85 88 139 53 4795 2 2 26.7

RC/RL149 81 86 148 53 4696 2 3.5 35

RC/RML10 84 87 120 58 4508 1.5 2.5 33.3

RML8/MUSTANG 65 68 127 41 3913 2 2.5 41.7

RC/ARUN2 68 89 140 55 3887 2 2 46.7

RML4/MUSTANG 66 68 93 30 3853 2 2 40 RC/RL192 74 82 152 67 3817 1.5 4 45

RC/RML8 83 85 145 60 3678 1.5 3 21.7

RL30/MUSTANG 64 64 114 44 3657 1.5 2 40

RC 76 83 125 50 3650 2 3 41.7

RL35/MUSTANG 64 66 118 46 3611 1.5 2 41.7

RL35/RML8 93 98 125 60 3529 1.5 2.5 25

NML2/MUSTANG 68 69 99 47 3452 2 2 45

RL19/RL133 81 84 128 50 3431 2.5 4 41.7

RML6/MUSTANG 64 65 106 29 3315 2.5 2.5 41.7

RML5/MUSTANG 68 69 102 28 3210 2.5 2 33.3

NML1/MUSTANG 63 66 106 34 3031 2 2 48.3 RC/MUSTANG 64 67 128 48 2857 2 2 38.3

RML18/MUSTANG 65 68 105 33 2243 3 2.5 35

ARUN2/MUSTANG 63 66 118 45 2065 2 2 46.7

Grand mean 129 55 5282

F test ** ** **

CV, % 11.57 19.18 20.78

LSD0.05 21.07 16.96 1773

Notes: RL=Rampur lines, RC= Rampur composite, CV=Coefficient of variation

Source: Annual Report 2063/64 (2006/07). National Maize Research Program (NMRP), Rampur

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Annexure 5: Mean performance of maize hybrids evaluated on Hybrid Observation Nursery (OBN) in 2067 winter at NMRP, Rampur Trt Genotypes Male

Flowering

Days

Female

Flowering

days

Plant

Height

(cm)

Ear

height

(cm)

No of

plant/plot

No. of

ear/plot

Rotten

ears

Grain yield

(kg/10m2)

Husk

covers

scores

Plant aspects

score

Ear aspects

scores

Insect

1 RML-4/NML-2 103 107 95 37 10.0 11.0 0.0 5.692 1.7 1.5 1.7 0.3

10 RML-55/RML-40 103 107 98 37 3.5 5.7 0.3 3.357 2.0 2.3 2.3 0.3

13 RL-99/RL-161 100 103 87 27 3.0 5.7 0.3 1.144 2.7 2.7 2.5 1.3

14 RML-40/RL-168 101 105 97 37 4.5 4.3 0.0 1.200 2.2 2.3 2.2 0.3

16 RL-106/RL-161 102 105 70 23 10.5 9.3 0.3 3.735 2.0 2.8 2.5 0.7

17 L-4/L-2 103 107 100 27 6.5 9.0 0.3 4.039 1.8 2.2 2.3 0.3

18 PUTU-17/L-1 105 106 90 35 3.0 3.7 2.7 1.350 1.7 2.2 1.8 0.3

19 PUTU-17/AG-27 104 107 95 37 8.5 12.7 0.0 3.077 1.7 1.7 1.8 0.3

20 ARUN-2/RML-8 95 98 95 37 8.0 8.3 0.3 2.565 2.0 3.0 2.5 1.7

21 PUTU-13/L-20 102 105 102 42 8.0 9.3 0.0 4.281 2.2 1.2 1.8 0.3

30 L-19/L-20 106 109 80 29 3.0 4.0 0.5 1.384 2.0 1.7 1.8 0.7

32 RML-4/RML-17 111 114 93 30 11.0 12.3 0.0 5.101 1.5 1.5 1.5 0.3

33 PUTU-20/AG-27 102 103 100 29 10.5 12.7 0.0 6.081 1.8 1.8 1.8 0.3

35 RL-182/RL-174 102 106 113 39 8.5 7.0 0.3 1.877 1.7 1.8 1.8 0.0

36 RC/RML-8 97 100 108 40 6.5 7.7 0.0 3.887 2.0 2.7 2.7 1.3

37 RL-105/RL-197 108 112 120 43 5.0 6.3 0.0 2.402 1.7 1.7 1.7 0.3

38 RAJKUMAR 97 99 108 40 11.5 9.0 0.0 4.984 2.0 2.0 2.0 2.0

39 PIONEER 30V92 101 105 95 34 10.5 9.0 1.7 4.612 2.5 2.0 1.8 2.3

40 PIONEER 30B07 100 103 92 35 12.5 13.7 0.0 7.961 1.7 1.5 1.5 0.7

41 Rampur composite 94 97 112 45 10.0 13.0 0.0 3.938 2.2 1.8 2.3 0.7

Mean 102 105 98 35 7.7 8.7 0.3 3.633 1.9 2.0 2.0 0.7

F test ** ** * NS ** ** NS ** NS ** ** NS

LSD0.05 5.32 6.09 23.53 17.30 2.55 4.17 1.87 2.56 0.69 0.69 0.56 1.54

CV, % 3.2 3.5 14.6 29.9 19.4 29.1 332.5 42.7 21.7 20.9 17.0 127.2

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Annexure 6: Agro-morphological characterization of 62 maize inbred lines in winter, 2010 at

NMRP, Rampur SN Name of

inbred lines

Days

to 50

%

tasselin

g

Days

to 50%

silking

ASI No. of

leaf

Leaf

orientati

on

(score

1-3)

Plant

height

(cm)

Plant

Aspec

t

(score

1-5)

Ear

Aspect

(score

1-5)

Ear

position

(cm)

Tassel

structu

re

(score

1-3)

Tassel

branch

Number

(No.)

Tassel

Length

(cm)

Tassel

color

(score

1-3)

Silk

color

(score

1-3)

1 RML-19 72 76 4 12.33 3 149.5 2 2 45.5 3 8.66 42.66 1 1

2 NML-2 71 75 4 12.33 2 191.5 1.5 1.5 80 3 10.33 38 3 2

3 RL-137 54 58 4 10.33 3 148.5 2 2 62.5 3 14 29.33 3 3

4 RML-4 59 62 3 11 1 120 1.5 2 55 2 7 35.33 3 3

5 RML-32 58 61 3 11.33 1 135.5 1.5 1.5 70.5 2 13.66 38.66 1 2

6 RML-17 74 77 3 12.33 2 162 3 2.5 67.5 2 9.66 33.33 3 2

7 RML-55 53 57 4 11.66 3 172 2.5 2.5 105.5 3 18 36.33 3 3

8 RL-29 53 55 2 11.33 3 147.5 1.5 2 82 3 13.33 28.66 3 3

9 RL-99 58 61 3 11 1 140.5 2.5 2.5 61 2 10.66 32 3 3

10 RL-72 57 62 5 10 3 99.5 2.5 2.5 60 2 5.33 38 3 2

11 RML-18 48 54 6 12.66 1 185 1.5 1.5 90.5 2 17 42 3 2

12 RML-6 71 75 4 12.33 3 165.5 1.5 2 65 3 8 40.66 3 1

13 RL-111 62 67 5 12.33 1 175 1.5 2 85.5 1 7 39 3 2

14 RL-189 71 74 3 13.33 3 203.5 2.5 2 100 2 13.66 45.33 3 2

15 RL-195 54 58 4 12 2 150 2.5 2.5 95.5 2 19.66 30.33 3 2

16 PUTU-17 58 62 4 11.66 1 131.5 2 2 62.5 2 13.33 41.66 3 2

17 PUTU-20 59 62 3 10.66 3 115 1.5 2 45 1 8.66 38.66 3 2

18 AG-27 62 66 4 12 3 170 3 3 75 3 11.66 42 3 2

19 RL-182 70 73 3 11.33 3 100 3 2.5 50.5 3 8 37.33 3 2

20 RL-174 70 74 4 10.66 1 140 2 2 55 3 10.66 35.33 2 2

21 RL-36 70 74 4 9.66 3 101.5 3 2.5 30 3 7.33 37.66 3 1

22 RML-36 68 72 4 10 1 99 2 2 50 2 7 36.33 2 2

23 RL-197 69 72 3 10 1 149.5 2.5 2.5 70 2 9 40.66 2 2

24 RL-180 53 58 5 12.33 3 211 3 2.5 91.5 3 16 43.33 3 2

25 RML-55 58 62 4 12.33 1 120 1.5 2 42 2 10.33 31 3 2

26 RL-5 59 61 2 12.33 1 150 1.5 2 75 2 14.66 32.33 3 1

27 RML-57 62 67 5 10 3 100 2.5 2.5 30.5 3 6 34.66 3 2

28 RML-6 62 66 4 12.66 3 107.5 1.5 2 25 3 8 36.66 3 2

29 RL-25 49 54 5 11.66 1 180 1.5 2 85.5 2 8.33 42.33 3 2

30 RL-170 61 55 -6 11.33 3 191 2 2 100 3 7 40.33 3 2

31 RL-154 61 66 5 10.66 1 143 1.5 2 60 3 10.33 34.66 3 1

32 RL-96 60 63 3 9.33 1 137 3 2.5 67.5 2 10 39.33 3 2

33 RL-102 62 65 3 9 1 100 2.5 2.5 45.5 2 6.66 36.33 3 2

34 RL-114 71 75 4 11.33 1 150 1.5 1.5 70 3 12 39.33 3 2

35 RL-153 61 65 4 11 1 140 1.5 2 66 2 9.33 35.33 3 2

36 RL-186 61 64 3 11.66 2 160 1.5 2 65.5 3 16 32.33 2 1

37 RL-150 62 66 4 11 1 136.5 3 2.5 55.5 3 14.33 31.66 3 2

38 RL-151 54 57 3 10.33 1 150 2 2 70 3 17.66 35.66 3 2

39 RL-86 57 61 4 11.33 3 160 1.5 2 80.5 3 4.33 42.66 2 2

40 RL-183 61 65 4 9 1 120 3 2.5 35 2 7 35.66 2 3

41 L-20 49 53 4 10.66 1 160.5 1.5 1.5 91.5 3 9.33 35 1 3

42 PUTU-22 61 64 3 10.33 3 140 1.5 2 55 3 9.66 36.33 3 1

43 PUTU-12 60 64 4 12 2 120 1.5 1.5 50 2 9 34 3 3

44 RL-85 49 53 4 11 1 152 1.5 1.5 60 3 14 46 3 1

45 L-10 71 76 5 11.33 3 95.5 2.5 2.5 35.5 3 6 30.33 2 1

46 L-3 59 62 3 12.33 2 110 1.5 2 55 2 7.33 37.33 3 3

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47 PUTU-21 69 72 3 10.66 2 122 1.5 2 52.5 2 13.33 37.66 3 3

48 RML-62 72 74 2 12.33 3 150 2 2 111 3 11.66 35.33 3 2

49 RL-105 56 50 -6 10 3 170 2 2 85 2 10 38 3 2

50 RML-8 47 50 3 10.66 3 166.5 1.5 1.5 80.5 3 11.33 45.66 3 2

51 RL-165 58 62 4 10.66 3 140 2.5 2.5 65 3 9.66 40.33 2 3

52 RL-94 62 66 4 10.33 3 150 2 2 75 2 18 40.66 1 1

53 RL-17 52 56 4 10 2 190 2 2 90 2 6.33 30.33 1 2

54 RL-19 53 56 3 10.66 3 140 3 2.5 50 3 10.33 44 3 3

55 RL-106 58 62 4 9.33 3 110.5 2.5 2.5 43.5 3 6.33 37.66 3 1

56 RL-84 54 57 3 10.66 3 165 2.5 2 70 3 7.33 41.66 1 1

57 RML-55 58 62 4 8.66 3 161.5 3 3 75.5 3 6.66 34 3 1

58 L-17 55 64 9 8.33 3 95 3 2.5 45 3 7.66 27.66 3 3

59 RL-194 58 62 4 10.33 3 120 2.5 3 60 3 9 33.66 1 3

60 RML-62 64 68 4 10.66 3 170 1.5 2 85 3 8.33 37 3 3

61 RC 68 72 4 11 2 169.5 1.5 1.5 90.5 2 9.33 45 1 1

62 Arun-2 64 68 4 10.66 2 154 2 2 80 3 9.33 42.33 1 1

Annexure 7: Mean performance of single cross maize hybrids in CVT conducted at NMRP, Rampur in winter 2006/07

Cross Days to 50%

anthesis

Days to 50%

silking

Plant height

(cm)

Ear height

(cm)

Grain yield (kg

ha-1)

RML4/NML2 67 68 153 72 7639

RML4/RML5 68 71 142 67 6567

RML4/RML8 64 67 142 73 6553

RML8/RL30-1 64 68 147 75 6043

NML1/RML8 66 72 157 77 7057 RL12/RML8 62 66 156 73 5671

RML6/RML8 66 71 167 78 7471

NML1/RML6 67 74 148 65 5265

RML18/RL17 69 74 142 57 6731

RL17/RL30-1 66 70 132 57 6641

RL29/RL30-1 61 64 153 73 5359

RML41/RL30-1 61 66 157 78 5803

GAURAV 64 70 155 78 6136

BIOSEED 9681 61 65 167 77 4091

PIONEER 30G10 64 70 142 68 5069

RAMPUR COMPOSITE 57 62 155 82 5497

Grand mean 64 69 151 72 6097 F test ** ** NS NS *

CV, % 3.5 3.8 10 14 17.4

LSD0.05 3.8 4.4 1841

Source: Annual Report 2063/64 (2006/07). National Maize Research Program (NMRP), Rampur

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Annexure 8: Grain yield and other important traits of hybrids evaluated in CVT at Rampur in

winter 2007 SN Genotypes Days to

50%

anthesis

Days to

50%

silking

Plant

height

(cm)

Ear

height

(cm)

Plant

aspect

(1-5)

TLB

(1-5)

HC

(1-5)

Grain

yield

(kg ha-

1)

1 RML4/NML2 75 78 172 98 1.5 1.8 1.7 8694

2 RML3/NML2 77 80 223 113 2.0 2.0 1.5 6774

3 NML1/RML8 72 75 200 97 2.0 2.0 2.0 6863

4 NML1/RML17 71 74 203 103 2.5 2.3 2.2 7551

5 RL125/RL169 67 70 212 105 2.2 1.7 2.3 5838

6 RML18/RML17 80 83 202 95 1.7 2.2 2.3 8548

7 RC/NML1 68 71 247 113 1.8 2.2 1.8 7461

8 RC/RML4 68 71 195 113 1.7 2.0 1.7 8097

9 RC/RML17 76 80 208 102 1.5 2.2 1.8 6518 10 RC/RML5 68 71 207 103 1.5 2.2 2.2 6318

11 RL30-1/RC 68 71 202 107 2.2 2.2 2.0 6002

12 RML4/ARUN2 70 73 182 92 2.2 2.0 2.2 7388

13 GAURAV 75 78 198 113 2.3 2.2 2.0 8577

14 PIO30G10 79 83 205 103 2.5 2.2 1.8 6630

15 BIOSEED9681 67 70 190 82 2.5 2.2 2.5 4169

16 Rampur composite 66 69 200 97 2.0 2.5 2.2 6257

Grand mean 72 75 203 102 2.0 2.1 2.0 6982

F test ** ** NS NS ** NS NS **

LSD0.05 5.92 5.84 42.91 25.91 0.39 0.53 0.67 1320

CV, % 5.0 4.7 12.7 15.2 11.8 15.1 19.9 11.3

RC=Rampur composite Source: Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur,

Chitwan, Nepal

Annexure 9: Grain yield and other traits of Indian and NMRP developed hybrids evaluated at

Rampur in winter 2007 SN Genotypes Days to

50%

anthesis

Days to

50%

silking

Plant

height

(cm)

Ear

height

(cm)

Plant

aspect

(1-5)

Ear

aspect

(1-5)

Husk

cover

Grain

yield

(kg ha-

1)

1 ADITYA 98 101 123 58 2.5 2.0 3.0 6011

2 BADSHAH 97 100 128 45 2.8 2.3 2.5 5465 3 BIOSEED 9681 96 100 130 50 2.8 1.8 2.8 4100

4 KESHAR KINGS 95 98 105 45 2.8 2.5 2.0 4099

5 SHUBHAM2 84 87 133 43 2.5 2.8 2.0 4805

6 SOW101 99 102 118 43 2.3 2.8 2.3 5348

7 RAJKUMAR 93 97 140 55 1.5 1.5 1.5 5136

8 PIONEER HYBRID 93.5 97 145 63 1.5 1.8 1.3 7269

9 NML1/RML8 102 109 115 43 1.3 1.3 1.5 6052

10 NML1/RML6 102 106 140 53 1.5 1.3 2.3 7195

11 PIONEER/RML8 94 97 115 43 1.8 2.0 1.0 6011

12 NML1/RML5 106 110 103 38 1.3 1.3 2.0 6997

13 NML4/NML2 105 108 130 60 1.5 1.3 1.8 7040 14 RML3/NML2 107 110 123 48 1.8 2.0 1.3 5379

15 GAURAV 104 107 110 45 1.5 1.3 1.3 7620

16 Rampur composite 91 94 120 45 2.3 2.5 1.3 3785

Source: Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur,

Chitwan, Nepal

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Annexure 10: Mean performance of hybrid maize genotypes on different traits evaluated in

Coordinated Varietal Trial (CVT) at NMRP, Rampur in 2010 EN Genotypes M

ale flo

wering da

ys

Female

flowering days

Anthesis silking interval (ASI)

Plant height (cm)

Ear height (cm)

No. of

plant/plot

No. of

ear/plot

Rotten ears

Grain yield

(kg ha-

1)

Husk cover

Plant

aspect

Ear aspects

Insects

1 RML-4/NML-1 107

110 3.7 90 35 11 10 1 4663 2 2 2 1 2 RL-111/RL-105 1

04

108 3.7 110

38 11 12 0 5190 2 2 2 0 3 RC/RML-8 9

0 94 4.0 10

8 47 10 11 1 4025 2 2 2 1

4 RL-111/RL-197 10

5

108 3.3 110

42 12 12 0 2758 2 2 2 0 5 CML-169/CML-167 1

03

106 2.3 87 32 11 12 2 4299 3 2 2 6 6 RML-4/NML-2 1

0

6

110 4.0 98 38 13 11 0 4607 2 2 2 2 7 CML-168/CML-187 1

00

103 3.7 108

52 11 12 3 3181 3 2 2 4 8 GRGTS1-7-1-3-

1/RL-84 100

103 3.3 100

38 11 13 1 4009 3 2 2 2 9 CML-312/CML-172 1

01

105 3.7 113

42 12 15 1 6439 2 2 2 6 10 ARUN-2-S1-10-1-2-

1/RL-84 93

97 3.7 103

43 12 15 1 4416 3 3 2 5 11 ARUN-2 S1-10-1-2-

2/RL-84 94

97 3.0 115

49 11 16 1 4707 2 3 2 4 12 RL-105/RL-197 1

03

107 3.7 140

52 11 15 3 4598 2 2 2 5 13 RC-S1-9-4-2-1/RL-

84 93

96 3.0 83 28 11 14 2 4769 2 2 2 5 14 RC-S1-9-4-3-1/RL-

84 99

102 3.7 85 30 12 13 0 4410 2 2 2 2 15 RC-S1-2-2-5-2/RL-

84 91

95 3.7 120

53 11 11 0 3889 2 2 2 1 16 RC-S1-4-1-5-2/RL-

84 99

103 4.0 108

38 9 10 0 1740 2 3 3 1 17 RC-S1-9-4-4-2/RL-

84

9

2

94 2.0 10

7

40 11 10 1 3856 2 2 2 3 18 CLQ-6602/CML-172 1

03

107 3.7 11

0

38 11 15 2 5172 3 2 2 3 19 CML-493/CML-172 1

01

105 4.3 88 25 10 10 0 2832 2 2 2 1 20 CML-168/CML-167 1

00

104 3.3 108

40 11 10 1 2663 2 3 3 3 21 CML-205/CML-172 1

01

105 3.7 100

33 12 14 0 5527 3 2 2 2 22 CLRCYQ-07/CML-

172 102

106 3.7 108

40 12 11 1 4092 2 2 2 1 23 CML-493/CML-167 9

6 99 3.3 10

7 40 10 12 2 4585 3 2 2 6

24 PIONEER-S1-5-2-1-2/RL-84

95

99 4.7 107

43 10 12 1 4843 2 2 2 2 25 PIONEER-S1-9-2-4-

1/RL-84 97

100 3.7 95 33 11 11 1 4270 3 3 3 2 26 PIONEER-S1-5-3-3-

2/RL-84 93

97 3.3 88 30 11 11 0 4270 2 3 3 1 27 GAURAV/P45-9 1

05

109 4.0 95 32 11 16 1 6082 2 2 2 2 28 PIONEER-S1-1-1-2-

2/RL-84 97

101 3.7 83 28 10 11 0 4339 2 2 2 3 29 RAJKUMAR 9

8

101 3.3 11

2

42 11 14 1 4458 3 2 2 5 30 30V92 1

00

104 4.0 107

40 11 13 1 4825 3 2 2 4 31 30B07 1

04

108 4.0 95 37 11 12 0 6228 2 2 2 2 32 Rampur composite 9

1 95 4.0 92 35 11 10 1 3523 2 2 2 1

Mean 99

102 3.59 103

39 11 12 1 4352 2 2 2 3 F test *

* ** NS * NS NS * * ** * * * *

LSD0.05 5.24

5.22 1.17 22.1

16.82

2.18 3.38

1.49

1.40 0.66

0.70

0.58

3.51 CV, % 0

.9

0.7 6.1 7.1 10.4

2.0 5.0 28.1

19.7 3.3 3.1 4.8 7.0

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Annexure 11: Performance of different maize varieties grown under varietal frontline demonstration

at NMRP, Rampur, Chitwan in winter season of 2009

SN Varieties Days to

50 % tasselling

Days to 50 % silking

Plant height (cm)

Ear height (cm)

Maturity date (days)

Plant aspect (1-5)

Ear aspect (1-5)

Grain yield (kg ha-1)

1 Gaurav 92 95 160 90 165 1.5 1.5 5324

2 RML-4/NML-2 91 95 140 80 154 2 2 7188

3 RML-3/NML-2 89 93 180 110 156 1.5 2 3557

4 Arun-2/RML-8 62 66 200 110 164 3 3 4708

5 C-1950 80 84 220 100 165 3.5 3 3393

6 C-1921 79 83 190 98 167 3 3.5 5142

7 C-6485 80 84 220 116 167 2.5 2 6293

8 Poshilo Makai 85 88 210 105 164 2.5 2.5 5731

9 Shitala 79 83 200 105 155 2 2 5857

10 Narayani 71 74 180 115 145 3 4 3959

11 Upahar 69 72 230 130 151 2.5 2 6749

12 Ramur Composite 70 74 200 130 149 2 2 4710

13 Arun-2 55 58 230 120 130 2 2 3759

14 Pool-17 50 53 180 70 128 3 3 3473

15 Arun-1 EV 54 58 230 122 125 3 3.5 3543

16 Manakamana-1 56 58 210 98 133 2.5 2 3801

17 Rampur SO3 FO6 76 80 200 110 135 2 2 5323 18 Across 9942/Across 9944 76 80 190 118 154 2 1.5 7721

19 Khumal yellow 65 69 200 110 150 2 3 3970

20 Deuti 75 79 205 102 158 1.5 2 4402

21 PoP 445 C1 55 59 210 99 137 3 2.5 2670

22 Pool-16 62 65 230 130 139 2 2.5 2403

23 S99TLYQ-A 74 78 220 130 152 2 2 4690

24 SOOTLYQ-1AB 75 78 170 110 155 1.5 1.5 5287

25 Rampur SO3 FO2 82 86 170 100 149 2.5 3 4758

26 Arun-4 65 69 200 108 135 2.5 3 3525

27 Gulmi-2 81 85 180 100 160 3 2.5 2129

28 CP 666 79 83 182 97 146 2 2 4282

29 CP 808 78 81 142 70 148 1.5 2 5101

30 RC/RML-8 72 76 190 98 154 2.5 3 4930

31 RL-176/RL-84 77 81 170 99 159 2 2.5 3893

32 RL-111/RL-189 86 90 154 80 161 1.5 1.5 5911

33 RML-62/RL-36 84 88 160 80 160 1.5 1.5 4253

34 RML-55/RML-40 69 74 198 107 164 2.5 2.5 4051

35 RC/RL-30-1 74 78 190 88 166 3 2.5 4642

36 Bioseed 9681 70 74 150 80 155 1.5 3 5751

37 Bioseed 76 80 172 92 158 2 2 6154

38 Manakamana-4 75 78 170 81 159 2 2 4796

39 Rampur-2 61 64 160 92 142 3.5 3 3851

40 S99TLYQ-A 76 79 198 92 149 2 2.5 3152

41 Across 9331 67 71 210 108 153 2.5 3 5429

42 Arun-1 59 63 250 70 138 2.5 3 2673

43 Manakamana-3 88 91 210 87 150 2 2 5036

44 Rampur SO3 FO4 84 88 135 82 156 1.5 1.5 4156 45 S99 TLYGHAYB (3) 78 84 175 100 149 2 2.5 4307

46 PoP 45/Pool-17 67 70 160 80 130 3 3 3291

47 Gaurav 89 92 140 82 165 1.5 1.5 5250

48 CP 666 82 85 150 81 152 1.5 1.5 7090

49 Bioseed 85 89 150 70 152 2 1.5 5293

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Annexure 12: Adjusted mean grain yield (kg ha-1) of multinational hybrids evaluated with collaboration of NMRP,

Rampur on three different locations in 2010-11 SN Genotypes Parwanipur Rampur Tarahara Mean

1 10V10 8607 5253 8560 7473 2 10V20 8284 6852 9769 8302 3 30B11 6935 10553 10411 9300 4 30V92 8772 7935 8671 8459 5 900M gold 8147 7572 10126 8615 6 9220 7729 7074 9705 8169 7 9681 7483 8544 9963 8664 8 Aditya-929 9041 6239 6982 7421 9 All rounder 8457 8037 8032 8175 10 Arun-2 6455 3854 4002 4770 11 Badshah gold 6367 8773 8347 7829 12 Big Boss 8711 8295 8213 8406 13 Bisco Bumper 9463 4641 7883 7329 14 Bisco Heera 8373 7050 4734 6719 15 Bisco Prince 10779 7198 8867 8948 16 Bisco x 81 8042 7945 7917 7968 17 Bisco x 92 8813 5599 8187 7533 18 Biscox 97 gold 7284 10179 9434 8966 19 C-1921 9619 5133 8009 7587 20 C-1945 9167 2735 9221 7041 21 C-1946 7969 7960 11720 9216 22 C-1950 9791 7646 8090 8509 23 C-6485 8947 6510 8107 7855 24 Challenge-1 5841 6586 8384 6937 25 Chhabili 7749 7045 7770 7521 26 Commando 8160 8969 10052 9061 27 CP-666 8431 7843 6296 7523 28 CP-808 8173 8453 5866 7497 29 CP-828 8508 5848 5688 6681 30 CP-838 8795 6945 5762 7168 31 Dekalb DK 984 10319 7639 5113 7690 32 Dekalb double 8107 5757 6572 6812 33 DKC 9081 9185 7969 8765 8640 34 DMH-7314 8447 6272 7014 7244 35 DMH-849 8689 6562 5563 6938 36 Don 1588 7797 6898 6604 7100 37 Early-2 8415 4807 7658 6960 38 Godavari-989 8382 5022 8082 7162 39 Hi-Shell 7647 8737 8105 8163 40 Kesherking-919 8219 6443 8423 7695 41 LY-558 7568 8821 6877 7755 42 LY-597 9972 7768 7761 8501 43 Madhur 9427 4384 5398 6403 44 MM-1107 9610 11562 6546 9239 45 MM-1109 10049 9222 5609 8293 46 MM-7705 7006 10151 7633 8264 47 Naya 940 10458 5983 6692 7711 48 NMH 1242 7952 9754 10775 9494 49 NMH 666 (Sandhya) 6652 7705 12185 8847 50 NMH 777 (Sunny) 8299 8162 9779 8747 51 NMH 731 9307 7128 9932 8789 52 NMH 909 4890 7365 9069 7108 53 P 3404 8898 8037 9969 8968 54 P 3522 9448 7370 8457 8425 55 P 3540 8922 7940 9085 8649 56 P 3785 8224 9892 7607 8575 57 P 3856 9612 11195 9607 10138 58 Pinnacle 9539 7067 10782 9129 59 Prabal 8277 10250 5288 7938 60 Proagro 4640 7799 6425 8292 7505 61 Proagro 4642 9633 7556 7745 8311 62 Proagro 4794 8146 6415 7635 7399 63 Proagro Sampanna 8972 8444 8019 8478 64 Rajkumar 9509 8233 8542 8762 65 Rampur composite 6307 5083 5850 5747

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66 RML-4/NML-2 6412 9155 9255 8274 67 Samrat 1133 5600 6587 7966 6718 68 Samrat 1144 7633 5486 7027 6715 69 Shaktiman 10223 8636 9261 9374 70 Super 900M 7997 6984 7564 7515 71 T.C.S.-9696 9545 5596 10581 8574 72 Tx 369 8506 9622 9626 9251 Mean 8397 7408 8100 7968 Replication * NS ** NS Genotypes NS ** ** ** Location ** Location x Genotypes * R2,% 45.9 51.4 68.4 53.3 CV,% 23.7 28.4 21.3 25.7

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Annexure 13: Combined analysis for grain yield and agronomic traits of maize genotypes under

heat stress screening trials conducted at three different locations (Rampur, Nepalgunj and Parwanipur) in 2010 summer

SN Genotypes Days to 50 % ASI (days)

Plant height (cm)

Ear ht. (cm)

Ear Len. (cm)

Ear dia. (cm)

Pl. Asp. (1-5)

Ear Asp. (1-5)

Grain Yield (kg ha-

1)

tasseling silking

1 RML-7 59 64 4 188 89 11 4 3 4 2719 2 RL---84 56 59 3 166 79 13 3 3 4 3675 3 RL--30-1 57 61 3 165 89 14 4 3 4 2890 4 PUTU-13 61 67 3 139 66 11 3 4 4 2671 5 Pool-17 45 47 3 161 70 11 3 3 4 3221 6 Arun-1EV 47 50 3 167 79 13 4 2 3 2943 7 Arun-4 57 62 3 171 75 15 4 2 3 3564

8 POOl -16 51 55 3 165 78 13 4 3 3 2927 9 Narayani 50 52 2 170 86 14 4 2 2 4110 10 Upahar 61 62 5 190 90 14 4 2 2 5228 11 Across-9331 53 56 2 173 90 14 4 2 2 3888 12 S 97 TLYHGA YB (3) 52 54 2 173 77 12 4 2 3 3069 13 Rampur composite 55 57 3 186 88 13 4 2 3 4086 14 Mana-4 56 57 4 182 87 14 4 3 3 5299 15 Arun-2 49 53 1 173 85 13 4 2 3 3409

16 OEHPW 58 61 2 203 97 15 4 2 2 4451 17 P501/RCO/P 502 SRCO 60 64 3 172 69 13 4 2 3 3419 18 BGBY POP 60 65 6 184 93 16 4 2 2 5110 19 S99TYQ-HGB 56 58 1 175 84 13 4 2 2 4194 20 Posilo Makai-1 59 64 4 187 90 15 4 2 2 4636 21 SO 1 SIWQ=3 64 66 3 164 67 13 4 3 3 3498 22 S 99 TLYQ-B 61 66 2 177 78 13 4 2 3 3419 23 Rampur SO 3 FO2 56 59 3 190 96 15 4 2 2 4345

24 Rampur SO 3 FO4 56 59 3 169 78 14 4 2 3 3982 25 Rampur SO3 FO6 57 60 4 192 92 14 4 2 2 4069 26 Rampur SO3 FQ-02 62 65 4 183 82 14 4 2 3 3910 27 BLSB SO7 F 10 54 56 5 174 87 14 4 2 2 4104 28 BLSB SO 7 F12 57 60 3 183 89 14 4 2 2 4466 29 TLBRS SO 7 F 14 55 58 2 190 87 14 4 1 2 4727 30 TLBRS SO 7 F16 56 60 1 188 102 14 4 1 2 5144 31 R POP-1 55 57 2 191 93 13 4 1 2 4411

32 R POP-2 50 53 2 196 101 13 4 2 3 4453 33 R POP-3 53 56 4 178 93 14 4 2 3 4194 34 R POP-4 51 53 3 191 99 14 4 1 2 4799 35 NML-1/NML-2 56 57 1 187 97 15 4 2 3 4780

36 RML-4/NML-2 64 67 2 166 78 14 4 3 3 3555 37 RML-8/RC 49 51 2 184 86 14 6 3 3 4958 38 Mana-3 58 60 4 191 98 15 4 2 2 4206 39 Rampur2 51 53 3 171 76 14 4 3 2 3375 40 Deeuti 57 60 5 192 98 16 4 1 3 4185

41 Pop-446 50 53 3 154 62 12 4 3 4 1883 42 Khumal Yellow 51 55 4 177 86 13 4 2 2 4366 43 Gulmi 54 56 2 234 122 15 4 2 3 3734 44 Resunga Composite 54 57 4 200 94 14 4 2 2 4631

Grand mean 55 58 3 180 86 14 4 2 3 3971 CV (%) 5.40 5.70 44.02 9.56 14.05 13.32 20.7 42.86 27.86 28.70 SD 4.39 4.83 1.12 15.29 11.25 1.19 0.40 0.61 0.66 771

LSD (0.05) 3.36 3.74 1.52 20.63 14.33 2.12 0.93 1.06 0.83 1299 F-test Genotypes (G) ** ** ** ** ** ** NS ** ** ** Location (L) ** ** * NS * * NS NS * ** Interaction (G × L) ** ** NS NA NA * NA * ** NS

ASI: anthesis-silking interval, pl:plant, ht: height, Len.: length,dia: diameter,Asp.:aspect and

Gr.:grain, NS=Non significant, NA:Not available, * significant at 1% level and **significant at 5%

level.

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Annexure 14: Analysis for grain yield and agronomic traits of maize genotypes under heat stress

screening trials conducted at Parwanipur in 2010 summer SN Genotypes Days to

50 % tasselling

Days to

50 % silking

Plant

height (cm)

Ear

height (cm)

Ear

length (cm)

Ear

diameter (cm)

Plant

aspect (1-5)

Ear

aspect (1-5)

Grain

yield (kg ha-

1) 1 RML-7 58 63 179 92 9 3 2 4 1839

2 RL-84 64 70 154 79 10 3 3 5 1426

3 RL-30-1 62 64 161 90 13 3 4 3 1889

4 PUTU-13 57 65 139 71 10 3 3 3 3834

5 Pool-17 46 47 150 65 12 3 2 4 2599

6 Arun-1EV 49 52 170 79 12 4 2 3 2739

7 Arun-4 48 50 175 84 13 4 2 3 2972

8 POOl -16 51 53 150 70 11 3 2 4 2046

9 Narayani 55 60 175 92 11 4 3 3 2847

10 Upahar 56 60 179 85 12 3 3 3 3509

11 Across-9331 52 55 185 101 13 4 1 2 3990

12 S 97 TLYHGA YB (3) 54 57 152 70 10 3 3 4 2014

13 Rampur composite 54 56 192 101 11 4 3 3 3262

14 Mana-4 58 61 177 102 12 3 3 2 3287

15 Arun-2 51 55 157 81 12 4 2 3 3666

16 OEHPW 56 59 213 108 15 4 1 2 3958

17 P 501/RCO/P 502 SRCO 59 64 157 60 10 3 3 4 1482

18 BGBY POP 60 65 178 98 16 4 2 2 4265

19 S 99 TYQ-HGB 56 58 156 80 10 3 3 3 2311

20 Posilo Makai-1 58 63 174 91 13 4 2 3 3265

21 SO 1 SIWQ=3 66 68 148 59 10 4 4 3 1881

22 S 99 TLYQ-B 64 69 177 79 10 3 2 4 1943

23 Rampur SO 3 FO2 57 60 188 87 15 3 2 3 3213

24 Rampur SO 3 FO4 58 61 154 75 12 3 3 3 2715

25 Rampur SO 3 FO6 58 61 192 96 12 4 2 3 3079

26 Rampur SO 3 FQ-02 68 71 164 75 11 3 3 4 2004

27 BLSB SO7 F 10 54 56 185 96 14 4 3 2 3908

28 BLSB SO 7 F12 58 59 181 90 13 3 2 2 3459

29 TLBRS SO 7 F 14 55 58 185 91 12 4 2 2 3629

30 TLBRS SO 7 F 16 57 60 205 114 16 4 1 2 4036

31 R POP-1 54 56 188 103 11 4 1 2 2339

32 R POP-2 53 54 198 110 13 4 1 2 4318

33 R POP-3 53 56 180 94 11 4 1 3 3845

34 R POP-4 52 53 201 113 13 3 1 2 3741

35 NML-1/NML-2 57 58 192 98 13 4 2 3 4400

36 RML-4/NML-2 68 71 153 75 13 4 3 4 2308

37 RML-8/RC 49 52 188 91 13 4 3 3 4336

38 Mana-3 58 60 195 110 14 3 2 2 4133

39 Rampur-2 51 54 165 76 11 3 2 3 2876

40 Deeuti 56 59 204 116 14 4 1 3 2960

41 Pop-446 50 53 139 62 9 3 3 5 1644

42 Khumal Yellow 51 55 159 83 12 4 1 2 3986

43 Gulmi 54 56 243 137 13 4 2 4 2880 44 Resunga Composite 52 56 193 95 11 4 2 3 3159

Grand mean 56 59 176 89 12 4 2 3 3045

CV (%) 6.75 7.40 11.50 15.95 14.99 510.11 42.27 31.23 28.84

SD 4.99 5.63 21.39 16.57 1.68 0.34 0.75 0.74 874

LSD (0.05) 7.56 8.75 40.78 28.60 3.65 79.54 1.81 1.79 1771

F-test ** ** ** ** * NS NS NS *

NS=Non significant, NA: Not available, * significant at 1% level and **significant at 5% level.

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Annexure 15: Analysis for grain yield and agronomic traits of maize genotypes under heat stress

screening trials conducted at Nepalgunj in 2010 summer SN Genotypes Days to

50 % tasselling

Days

to 50 % silking

Plant

height (cm)

Ear

height (cm)

Ear

length (cm)

Ear

diameter (cm)

Plant

aspect (1-5)

Ear

aspect (1-5)

Grain yield

(kg ha-1)

1 RML-7 60 66 191 78 11 3 3 3 2781

2 RL-84 56 60 171 73 13 4 4 4 3523

3 RL-30-1 62 64 155 73 16 4 3 4 4120

4 PUTU-13 64 72 126 54 10 4 4 4 2507

5 Pool-17 46 47 157 63 7 2 4 4 3882

6 Arun-1EV 49 52 169 72 14 4 2 4 4263

7 Arun-4 49 51 161 67 17 4 2 2 4453

8 POOl -16 56 58 174 82 15 4 3 3 4407

9 Narayani 54 58 181 73 16 4 1 2 5718

10 Upahar 56 60 190 85 17 4 1 1 7376

11 Across-9331 55 58 170 78 15 4 1 2 5163

12 S 97 TLYHGA YB (3) 54 57 173 65 15 4 1 2 4590

13 Rampur composite 54 56 184 79 14 4 1 1 5209

14 Mana-4 62 63 176 72 16 4 2 2 6377

15 Arun-2 52 55 181 78 13 4 2 2 3734

16 OEHPW 56 59 189 82 16 4 2 1 5921

17 P 501/RCO/P 502 SRCO 58 64 178 66 15 4 1 2 5362

18 BGBY POP 58 63 190 86 17 4 1 2 7370

19 S 99 TYQ-HGB 57 58 192 85 14 4 1 2 5642

20 Posilo Makai-1 58 63 189 78 17 5 2 2 5118

21 SO 1 SIWQ=3 66 68 170 63 17 4 2 3 7174

22 S 99 TLYQ-B 61 66 171 69 14 4 1 2 4619

23 Rampur SO 3 FO2 57 60 179 84 16 4 1 1 6379

24 Rampur SO 3 FO4 59 63 173 70 16 4 1 3 5574

25 Rampur SO 3 FO6 59 64 190 82 17 5 3 2 6127

26 Rampur SO 3 FQ-02 68 71 190 74 16 4 1 2 6305

27 BLSB SO7 F 10 53 56 160 76 15 4 2 2 4482

28 BLSB SO 7 F12 58 59 172 77 14 4 1 2 6188

29 TLBRS SO 7 F 14 55 59 192 79 16 4 1 2 5594

30 TLBRS SO 7 F 16 56 60 182 85 15 4 1 2 6158

31 R POP-1 54 56 180 79 15 4 2 2 6203

32 R POP-2 52 55 200 90 11 4 3 4 4371

33 R POP-3 53 56 173 90 16 4 3 3 5202

34 R POP-4 52 53 184 82 15 4 2 2 6339

35 NML-1/NML-2 57 58 185 95 18 4 1 3 6318

36 RML-4/NML-2 66 70 164 68 16 4 4 3 4802

37 RML-8/RC 49 52 184 81 16 4 1 2 6356

38 Mana-3 58 60 185 81 15 4 2 3 5134

39 Rampur-2 51 54 171 72 16 4 3 1 3880

40 Deeuti 56 60 185 87 18 4 2 3 5788

41 Pop-446 50 53 150 51 11 4 3 4 2840

42 Khumal Yellow 50 55 181 80 14 4 1 1 5439

43 Gulmi 54 58 221 98 17 4 3 2 5309

44 Resunga Composite 52 55 214 90 17 4 3 3 5734

Grand Mean 56 59 178 77 15 13 2 2 5223

CV (%) 5.7 5.8 6.2 12.1 13.7 462.2 19.9 24 14.8

SD 4.88 5.49 16.07 9.76 2.21 0.458 1.00 0.94 1175

LSD (0.05) 6.43 6.85 22.17 18.80 4.14 120.53 0.76 1.03 1562

F-test ** ** ** ** ** NS ** ** **

NS=Non significant, NA: Not available, * significant at 1% level and **significant at 5% level.

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Annexure 16: Analysis for grain yield and agronomic traits of maize genotypes under heat stress

screening trials conducted at Rampur in 2010 summer SN Genotypes Days to

50 % tasselling

Days to

50 % silking

Plant

height (cm)

Ear

height (cm)

Ear length

(cm)

Ear

diameter (cm)

Plant

aspect (1-5)

Ear

aspect (1-5)

Grain yield

(kg ha-1)

1 RML-7 54 56 194 97 13 4 3 4 3538

2 RL-84 51 54 173 87 15 4 2 3 6075

3 RL-30-1 60 60 179 104 13 4 3 4 2663

4 PUTU-13 62 65 153 74 14 4 4 4 1671

5 Pool-17 44 47 176 83 13 4 4 4 3181

6 Arun-1EV 44 47 162 85 13 4 3 3 1826

7 Arun-4 43 43 179 74 14 4 3 3 3269

8 POOl -16 44 46 171 82 12 4 4 4 2329

9 Narayani 43 47 155 92 15 4 1 3 3766

10 Upahar 55 58 201 101 14 5 3 2 4799

11 Across-9331 53 55 165 93 13 4 3 3 2510

12 S 97 TLYHGA YB (3) 48 50 196 97 12 4 3 3 2603

13 Rampur composite 58 60 182 84 14 4 3 5 3786

14 Mana-4 49 50 193 87 14 4 3 4 6231

15 Arun-2 44 49 181 95 12 4 4 4 2827

16 OEHPW 61 65 208 103 15 4 3 4 3476

17 P 501/RCO/P 502 SRCO 62 64 180 80 13 4 3 4 3414

18 BGBY POP 63 66 184 96 14 4 2 3 3694

19 S 99 TYQ-HGB 55 55 177 88 15 4 1 2 4629

20 Posilo Makai-1 61 65 199 101 15 4 2 2 5527

21 SO 1 SIWQ=3 60 62 173 80 11 4 4 4 1439

22 S 99 TLYQ-B 59 63 185 86 14 4 2 4 3694

23 Rampur SO 3 FO2 56 59 204 118 15 4 3 2 3443

24 Rampur SO 3 FO4 51 55 181 88 14 4 2 2 3657

25 Rampur SO 3 FO6 54 56 193 97 13 4 2 2 3001

26 Rampur SO 3 FQ-02 51 54 195 99 14 4 2 3 3421

27 BLSB SO7 F 10 54 56 178 88 14 4 2 2 3922

28 BLSB SO 7 F12 57 61 197 102 14 4 2 3 3750

29 TLBRS SO 7 F 14 54 56 195 91 15 4 1 1 4958

30 TLBRS SO 7 F 16 57 60 177 108 13 4 2 1 5239

31 R POP-1 57 60 205 99 14 5 1 1 4693

32 R POP-2 46 49 191 102 15 4 2 3 4672

33 R POP-3 54 56 181 97 17 5 2 3 3535

34 R POP-4 50 53 188 102 14 4 2 2 4315

35 NML-1/NML-2 54 56 185 99 14 4 3 3 3623

36 RML-4/NML-2 58 59 181 91 14 4 2 3 3554

37 RML-8/RC 49 51 180 85 14 5 4 4 4183

38 Mana-3 58 60 192 104 15 4 1 1 3350

39 Rampur-2 51 52 178 81 16 4 3 3 3369

40 Deeuti 59 62 187 92 15 4 2 2 3807

41 Pop-446 51 53 172 74 15 4 3 4 1164

42 Khumal Yellow 52 54 190 97 13 4 3 2 3673

43 Gulmi 54 56 237 132 15 4 2 3 3013

44 Resunga Composite 57 61 195 97 16 4 1 2 5000

Grand mean 53 56 185 93 14 4 2 3 3643

CV (%) 6.8 7.1 9.7 16.3 17.4 6.28 48.5 35.5 39.2

SD 5.65 5.76 14.77 11.27 1.12 0.24 0.93 0.93 1111

LSD (0.05) 4.23 4.67 19.85 16.01 2.70 0.49 1.18 1.04 1769

F-test ** ** ** ** * NS ** ** *

NS=Non significant, NA: Not available, * significant at 1% level and **significant at 5% level.

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Annexure 17: Grain yield and other traits of hybrids evaluated in CFFT in 2007 winter Genotypes Days to

50%

anthesis

Days to

50%

silking

Plant

height

(cm)

Ear

height

(cm)

Ear

aspect

(1-5) TLB (1-

5

Husk

cover (1-5

Grain yield

(kg ha-1)

RML4/NML2 68 71 210 110 1.5 1.5 1.5 8183 NML1/RML8 78 81 175 70 2 2 2 6705 RML3/NML2 80 83 210 110 2 1.5 2 6215 RL30-1/RC 66 69 190 105 1.5 2 2 5395 GAURAV 72 75 190 95 2 2 2 6577 PIO30G10 81 84 190 90 2 2.5 2 5276 Rampur composite 73 76 215 105 2 2.5 2 5235 Source: Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur, Chitwan

Annexure 18: Mean grain yield and other characters of hybrids tested in CFFT at Rampur, Parwanipur and Belachapi in 2008

SN Genotypes 50% silking days Plant height (cm) Ear height (cm) Grain yield (kg ha-1)

1 Gaurav 107 130 55 6368 2 Pioneer3087 106 135 46 6198 3 Rajkumar 107 134 48 5817 4 RML4/NML2 111 133 52 5677 5 RML3/NML2 110 137 57 5178 6 RML8/RC 102 136 52 5129 7 Aditya 929 102 119 42 4493 Grand mean 107 132 50 5214 F test NS NS NS NS LSD0.05 5.9 20 15.4 2642 CV,% 3.7 10 20 32 Source: Annual Report 2065/66 (2008/09). National Maize Research Program (NMRP), Rampur, Chitwan

Annexure 19: Performance of different Indian and NWRP maize hybrids tested at Pithuwa,

Chitwan in 2007 SN Genotypes Text

color

Grain

texture

Silking

days

Maturity

days

Plant

height

(cm)

Ear

height

(cm)

Grain

yield (kg

ha-1)

Husk

cover

(1-5)

1 Aditya Yellow SF 116 156 175 90 6886 1

2 Badshah Yellow SF 108 153 165 80 7256 3

3 Bioseed 9681 Yellow SF 111 154 165 62 6660 2.5 4 Keshar king Yellow SF 106 151 170 100 7626 2.5

5 Shubham 2 Yellow SF 108 153 168 70 7589 2.5

6 SOW 101 Yellow SF 106 151 190 85 8070 2

7 Rajkumar Yellow F 109 153 180 80 8301 1

8 Pioneer 30G10 Yellow SD 111 155 175 90 7885 1

9 NML1/RML8 Yellow F 118 155 175 75 6664 1

10 NML1/RML6 Yellow F 115 159 178 85 6293 2

11 Pioneer 30G10/RML8 Yellow SF 108 153 180 85 6979 1

12 NML1/RML5 Yellow F 123 160 165 80 6367 1

13 RML4/NML2 Yellow F 119 157 170 95 5701 1

14 RML3/NML2 Yellow F 124 160 166 80 4776 1

15 Gaurav Yellow F 121 157 170 100 5183 1 16 Rampur composite Yellow F 114 156 180 95 4035 1

17 Raja NMH 909 Yellow F 120 158 165 70 6590 2

18 Hira Deluxe Yellow SD 120 158 160 85 6960 2.5

19 Bisko Bumper Yellow SF 122 159 178 75 6182 1

20 Makka 940 Yellow SD 120 158 185 110 6664 2

21 Kanak Super Deluxe Yellow SD 119 158 175 85 6553 1

22 Pioneer 30G07 Yellow SF 121 160 170 90 7330 1 Source: Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur, Chitwan, Nepal

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Annexure 20: Response of hybrid maize (RML4/NML2) to different plant densities and nitrogen

levels on growth and grain yield at Rampur in 2007 winter Density

(D)

Nitrogen

(N)

Plant

height

(cm)

Ear

height

(cm)

Ear

length

(cm)

Kernel/row

(number)

Kernel

rows/ear

(number)

Thousands

grains

weight (g)

Grain

yield

(kg ha-1

)

30 x 60 60 142 52 14.1 32.6 14.1 312.9 3130

30 x 60 90 144 56 15.8 31.8 13.1 417.6 4520

30 x 60 120 149 53 15.8 30.6 13.9 414.9 4030

25 x 60 60 148 56 14.6 29.4 14.3 329.3 3890

25 x 60 90 145 55 16.2 33.5 13.5 415.0 4770

25 x 60 120 150 62 16.2 33.0 13.1 394.7 4840

20 x 60 60 132 48 14.0 29.9 13.1 399.5 4770

20 x 60 90 146 49 16.3 31.3 13.6 400.1 4980

20 x 60 120 149 57 16.1 32.4 13.7 423.5 4420

Grand mean 145 54.1 15.4 31.6 13.5 389.7 4360 Density NS NS NS NS NS NS NS

Nitrogen NS NS * NS NS * *

Density x Nitrogen NS NS NS NS NS NS NS

LSD0.05 for D

LSD0.05 for D 0.8 43.4 0.63

LSD0.05 for D x N

CV, % 6.09 12.53 5.57 8.05 5.79 11.18 14.47

Source: Annual Report 2064/65 (2007/08). National Maize Research Program (NMRP), Rampur, Chitwan

Annexure 21: Mean grain yield of different hybrids with different plant spacing in winter 2008 Genotypes Spacing (cm) Grain yield

(kg ha-1) 75 x 25 75 x 20 60 x 25 60 x 20 50 x 20

NML1/NML2 4235 4565 6438 6657 5603 5499

NML1/RML8 3791 4245 5707 5431 5374 4909

RML4/NML2 5224 5296 6516 7460 7056 6304

Mean 4416 4702 6220 6516 6001 5571

Source: Annual Report 2065/66 (2008/09). National Maize Research Program (NMRP), Rampur, Chitwan

Annexure 22: Effects of different density and nitrogen levels on hybrid maize (RML4/NML2) at Rampur in 2008

Treatments Plant

height

(cm)

Ear

height

(cm)

Ear

length

(cm)

Kernels/rows

(no.)

Kernel

rows/ear

(number)

Thousands

grains

weight (g)

Stover

yield (kg

ha-1)

Grain

yield (kg

ha-1)

30 x 60 cm 168 78 13.7 29.3 15.1 460 12250 8310 25 x 60 cm 168 79 13.1 28.2 15.0 459 12320 8510 20 x 60 cm 172 82 12.4 26.9 15.5 458 13230 9100 F test NS NS NS * NS NS * * LSD0.05 2.01 0.61 660 N=60 kg/ha 169 81 13.1 28.1 15.0 456 12180 8030 N=90 kg/ha 168 78 13.0 28.3 15.3 453 12800 8900 N=120 kg/ha 167 80 13.1 28.1 15.3 470 13820 8740 F test NS NS NS NS NS * * * LSD0.05 18.3 980 610 CV, % 3.91 3.88 5.92 8.13 4.14 5.05 16.3 13.31 Source: Annual Report 2065/66 (2008/09). National Maize Research Program (NMRP), Rampur, Chitwan,

Nepal

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30

Annexure 23: Details of the plant density study on hybrid maize in winter 2008 at Rampur Genotypes Plant

spacing

(cm)

50%

anthesis

50%

silking

Plant

height

(cm)

Ear

height

(cm)

Root

lodging

(%)

Shoot

lodging

(%)

Grain

yield

(kg ha-1)

NML1/NML2 75 x 25 65 73 209 110 3 1 4235 75 x 20 64 72 226 124 1 2 4565

60 x 25 66 74 223 132 1 2 6438

60 x 20 66 72 227 124 0 6 6657

50 x 20 67 74 223 124 3 3 5603

NML1/RML8 75 x 25 66 74 197 117 1 1 3791

75 x 20 65 72 216 114 0 0 4245

60 x 25 66 72 201 115 2 1 5707

60 x 20 66 74 206 119 1 2 5431

50 x 20 66 73 217 120 1 3 5374

RML4/NML2 75 x 25 64 71 219 137 0 0 5224

75 x 20 63 70 217 118 0 1 5296

60 x 25 65 71 208 123 0 2 6516 60 x 20 64 70 225 131 1 0 7460

50 x 20 63 69 216 118 0 0 7026

Grand mean 65 72 215 122 5571

Variety (A) * * NS NS **

Population (B) NS NS NS NS **

A x B NS NS NS NS NS

LSD0.05 3 4 19 298 1119

CV, % 4.2 5.2 6.0 8.1 12

Source: Annual Report 2065/66 (2008/09). National Maize Research Program (NMRP), Rampur,

Chitwan, Nepal

Annexure 24: Interaction effect of Hybrid and spacing on grain yield (kg ha-1

)

Factor A (Hybrid)

Factor B (Population levels)

Average (Hybrids)

75 × 15 cm (88,888 plant/ha)

75 × 20 cm (66,666 plant/ha)

75 × 25 cm (53,333 plant/ha)

60 × 20 cm (83,333 plant/ha)

NML-1/NML-2 4590 5490 5.43 5060 5140

RML-8 /RC 4350 5390 5.05 3750 4640

RML-4 /NML-2 6190 6077 6.05 6030 6240

Average (spacing) 5040 5860 5.51 4950 5340

Annexure 25: Interaction effect of inbred and nitrogen on grain yield (kg ha-1

)

Factor A (Inbred line)

Factor B(Nitrogen level) Average

(Inbred lines) 120:60:40 NPK (kg ha-1)

150:60:40 NPK (kg ha-1)

200:60:40 NPK (kg ha-1)

NML-2 1420 1250 1440 1370 RL-105 1190 820 1170 1060

RL-111 1380 1650 1040 1360

RML-4 1220 800 1200 1070

Average (Nitrogen level)

1300 1130 1220 1220

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Annexure 26: Treatment combination for N x D experiment SN Treatments Nitrogen (N)

kg ha-1 Plant population

(ha) Spacing

(cm) Remarks

1 2 3 4

5 6 7 8 9

D1N1 D1N2 D1N3 D2N1

D2N2 D2N3 D3N1 D3N2 D3N3

60 90 120 60

90 120 60 90 120

55,555 plants 55,555 plants 55,555 plants 66,666 plants

66,666 plants 66,666 plants 83,383 plants 83,383 plants 83,383 plants

30 x 60 30 x 60 30 x 60 25 x 60

25 x 60 25 x 60 20 x 60 20 x 60 20 x 60

10 t of compost and 40:30 kg/ha of P2O5 and K2O were applied basally in all the plots.

Annexure 27: Effect of nitrogen rates on the growth and yield characters of maize planted at

varying densities (N x D) in the years 2009/10 at NMRP farmland, Rampur Treatments Treatment

combination Plant ht, cm

Ear ht, cm

Ear length

(cm)

No. of kernel

rows/ ear

No. of kernel/

row

Stover weight

(kg ha-1)

1000 grain

weight (g)

Grain yield (kg

ha-1)

T1 T2 T3 T4 T5 T6

T7 T8 T9

D1N1 D1N2 D1N3 D2N1 D2N2 D2N3

D3N1 D3N2 D3N3

148.66 168.00 168.33 162.66 174.66 173.00

165.33 170.66 177.66

59.66 70.43 78.00 67.33 79.66 73.33

68.33 74.00 79.66

12.26 14.80 14.06 13.33 13.53 14.33

12.80 13.53 14.06

13.33 16.13 15.86 15.00 16.13 16.53

15.60 16.00 16.40

27.53 32.53 32.80 30.13 32.20 32.60

29.06 30.60 33.06

6060 8760 8700 6830 9100 8900

6900 8800 8660

410.66 445.33 456.33 425.33 456.66 477.00

410.66 448.33 456.33

8980 11010 10990 10170 12100 11470

9130 12160 12040

Grand mean 167.66 72.27 13.63 15.66 31.17 8080 442.96 10890 CV (%) 4.61 9.46 2.51 3.89 5.86 5.42 2.44 8690 F-test Density, a

Nitrogen, b

a x b

* **

NS

NS *

NS

NS **

*

* **

*

NS **

NS

NS **

-

** **

NS

NS **

NS LSD (0.05) a

b a x b

7.76 8.46 -

- 7.00 -

- 0.355 0.512

0.678 0.626 1.085

- 1.875 -

- 448 -

10.06 10.06 -

- 0.846 -

**=Highly significant, *= significant and ns= non-significant.

Annexure 28: Effect of nitrogen rates on the growth and yield characters of maize planted at

varying densities (N x D) in the years 2009/10 at NMRP farmland, Rampur.

Density Nitrogen Grain yield (kg ha-1

)

60 x 30 60:60:40 2747

60 x 30 120:60:40 3857

60 x 30 180:60:40 3870 60 x 25 60:60:40 2790

60 x 25 120:60:40 4437

60 x 25 180:60:40 4697

60 x 20 60:60:40 3113 60 x 20 120:60:40 4480

60 x 20 180:60:40 4360

Grand mean 3816 CV, % 18.6

LSD0.05 1.2 Source: Annual Report 2067/68 (2010/11). National Maize Research Program (NMRP), Rampur, Chitwan,

Nepal

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Annexure 29: Screening of maize inbreds against Northern Leaf Blight (NLB) at Rampur in

2006 SN Inbred 1st score (%) blighted 2nd score (%) blighted 3rd score (%) blighted 1 RL163 21.5 40 47.5 2 RML50 15 47.5 47.5 3 RL100 15 37.5 47.5 4 RL41 12.5 40 45 5 RL162 10 42.5 52.5 6 RML12 10 35 37.5 7 RML4 17.5 40 40 8 RL141 22.5 45 55 9 RL143 15 45 50 10 RL47 8 20 35 11 RL169 15 40 40 12 RML18 12.5 32.5 35 13 RL98 20 42.5 55 14 RL19 17.5 40 52.5 15 RML9 9 20 40 16 RL134 8.5 37.5 42.5 17 RL9 10 35 42.5 18 RML13 17.5 35 35 19 RL35A 16.5 37.5 37.5 20 RL106 12 27.5 32.5 21 RML36 6.5 15 27.5 22 RML5 7.5 25 32.5 23 RL194 7.5 22.5 32.5 24 RL168 11 37.5 45 25 RML41 17.5 42.5 55 26 RML11 21.5 40 45 27 RL171 18 37.5 40 28 RL167 9.5 27.5 35 29 RL116 13.5 35 42.5 30 RML3 5 32.5 32.5 31 Yellow pop corn 16 47.5 62.5 32 RML46 12.5 45 52.5 33 RL140 13.5 42.5 50 34 RL95 17.5 45 40 35 RL118 20 42.5 42.5 36 NML1 6.5 22.5 27.5 37 RL84 8 35 40 38 RL111 11.5 35 47.5 39 RL177 6.5 35 40 40 RL99 9 37.5 40 41 RL14 5 32.5 37.5 42 RL57 10 30 37.5 43 NML2 12 37.5 45 44 RML2 10 40 45 45 RML59 10 35 40 46 RML40 6.5 32.5 37.5 47 RML6 5.5 45 47.5 48 RL31 10 40 40 49 RL161 7.5 22.5 35 50 RL110 5 27.5 35 51 RML19 8.5 35 37.5 52 RML55 5 30 35 53 RL17 6.5 27.5 37.5 54 RL30-1 5 25 37.5 Grand mean 11.7 35.2 41.6 F test ** ** ** LSD0.05 6.5 10.6 12.8 CV, % 28 15 15.4

Source: Annual Report 2063/64 (2006/07). National Maize Research Program (NMRP), Rampur

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Annexure 30: Response of maize genotypes against major diseases at NMRP, Rampur in 2009

summer SN Genotypes BLSB Score (1-5) % rotten

ears

H.

maydis

(1-5)

Late

wilted

plants

(%)

1st

score

2nd score 3rd

score 1 NML-1 3.3 3.8 4 52 2.3 50 2 NML-2 2.5 2.8 3.3 38 1.8 56 3 RML-3 2.3 2.8 2.8 10 2.3 40 4 RML-4 3.3 3.8 3.8 7 1.8 56 5 RML-5 2 2.5 2.5 8 2 51 6 RML-8 1.5 2 2.5 82 2 32 7 RL-105 2.8 3 3.5 54 2.3 54 8 RL-84 2.5 2.8 3.3 31 2.5 40 9 RML-17 3 3.3 3.8 67 2.5 28 10 RML-18 2.5 3.3 3.8 63 2.3 58 11 PUTU-13 1.5 2.5 2.8 48 2 35 12 RL-30-1 2.5 2.8 3.3 44 3 67 13 CML-161 3.3 3.8 4.8 60 3 56 14 CML-187 3 3.5 4 50 2 50 15 CML-172 3.5 3.8 4.8 23 3.3 47 16 AG-27 2 2.3 3 6 2 45 17 L-1 2 2.3 2.5 21 2.3 78 18 L-7 1.8 2.3 2.3 37 2.3 44 19 POOL-17 3.5 4 4.3 88 2.3 44 20 ARUN-1EV 3.3 3.5 4.3 85 3.3 47 21 ARUN-4 2.8 3.3 4.3 37 3.3 28 22 POOL-15 3 3.8 4.3 111 1.8 38 23 POOL-16 2.8 3.3 4.3 53 2.3 38 24 NARAYANI 3 3.3 3.3 43 2.5 58 25 UPAHAR 2 2.8 3 41 2.5 49 26 ACROSS-9331 2.5 2.5 3.5 47 2.5 47 27 S97TLYGHAYB(3) 3 3.5 3.5 64 2 60 28 RAMPUR COMPOSITE 2.8 3.8 3.8 28 2.3 55 29 MANAKAMANA-4 3 3.5 3.8 74 2.3 63 30 ARUN-2 3.8 3.5 4.3 66 2.5 38 31 AC 9942/AC 9944 2.3 2.8 3 26 3 31 32 OEHPW 2 3 3.3 21 2.3 42 33 P501SRCO/P502SR 3.3 3.5 4 39 2 43 34 BGBYPOP 2.5 2.8 3 26 2 59 35 S99TLYQ-AB 2.8 2.5 3 41 2.3 49 36 S99TLYQ-HG-B 2.3 3 3.5 53 2.8 46 37 POSHILO MAKAI-1 2.5 3 3.3 48 2.8 74 38 SO1SIWQ-3 2.8 3 3.3 16 2.3 73 39 S99TLYQ-B 3.3 3.3 3.8 29 2.8 61 40 RAMPUR SO3FO2 2 2.5 2.8 23 1.8 61 41 RAMPUR SO3FO4 2.3 2.5 2.8 22 1.5 46 42 RAMPUR SO3FO6 2.5 2.8 3 33 2.5 51 43 RAMPUR SO3FQ02 2 2.8 2.8 42 2.5 54 44 BLSBRSO7F10 2 2.5 2.5 31 1.8 44 45 BLSBRSO7F12 2 2.3 2.5 24 2 38 46 TLBRSSO7F14 2.3 2.5 3 22 2 42 47 TLBRSSO7F16 2.3 2.5 3 28 2 44 48 R POP-1 3.3 3.5 3.8 33 2.3 50 49 R PO-4 2 2.8 3.5 6 2.5 57 50 R POP-2 2.5 3.3 3.5 24 2.5 39 51 R POP-3 2 2.3 3.5 34 1.8 39 52 NML-1/NML-2 1.8 2.3 2.5 15 1.8 18 53 RML-4 /NML-2 2 2.3 2.3 29 1.8 35

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54 RML-3 /NML-2 2.5 2.8 3 25 2 26 55 RML-8 / RC 2.8 3 3.5 8 2 37 56 MANAKAMANA-3 2.3 2.5 3 20 2 48 57 MANAKAMANA-1 3 3.5 4.3 65 2.5 51 58 DEUTI 2.8 3 3.8 44 2.3 54 59 POP-446 2.8 3.5 3.8 50 3.3 47 60 KHUMAL YELLOW 3.5 4 4.5 27 2.8 61 61 GULMI-2 2.8 3.5 3.8 36 3.3 47 62 RESUNGA COMPOSITE 2.8 3.5 3.8 35 3.3 43 63 L-13 1.8 2.3 2.5 8 1.8 62 Grand mean 2.6 2.9 3.4 38.4 2.3 48 F-test ** ** ** ** ns ns CV (%) 19.96 17.6 18.78 58.9 27.9 46.8 LSD (0.05) 1.02 1.05 1.27 45.25 1.29 45.8

Annexure 31: Combined analysis on pest infestation of maize genotypes under heat stress

screening trials conducted at three different locations (Rampur, Nepalgunj and

Parwanipur) in 2010 summer SN Genotypes Wilt % Stem borer

% S N Genotypes Wilt % Stem

borer %

1 RML-7

20.17 3.83 26 Rampur SO 3 FQ-

02 2.33 0.0

2 RL-84 6.0 0.00 27 BLSB SO7 F 10 3.17 1.50 3 RL-30-1 1.17 5.0 28 BLSB SO 7 F12 6.0 0.83

4 PUTU-13 11.0 2.17 29 TLBRS SO 7 F 14 2.0 0.0 5 Pool-17 12.83 8.33 30 TLBRS SO 7 F 16 7.17 0.0 6 Arun-1EV 6.17 1.67 31 R POP-1 8.33 0.0 7 Arun-4 2.0 3.17 32 R POP-2 9.0 0.0 8 POOl -16 9.0 2.67 33 R POP-3 18.33 1.17 9 Narayani 3.83 0.83 34 R POP-4 8.33 0.83 10 Upahar 1.67 6.83 35 NML-1/NML-2 14.50 2.50 11 Across-9331 2.50 0.0 36 RML-4/NML-2 4.17 8.0

12 S 97 TLYHGA YB (3)

7.0 1.83 37

RML-8/RC 3.0 0.0

13 RC 8.50 0.0 38 Mana-3 7.0 0.0 14 Manakamana-4 1.50 7.17 39 Rampur-2 4.0 2.17 15 Arun-2 6.33 1.17 40 Deeuti 4.67 0.0 16 OEHPW 7.0 0.72 41 Pop-446 10.33 0.0 17 P 501/RCO/P

502 SRCO 8.83 5.83

42 Khumal Yellow

6.33 0.0

18 BGBY POP 6.00 0.83 43 Gulmi 1.50 2.0 19 S 99 TYQ-

HGB 1.83 2.0

44 Resunga Composite

11.17 0.0

20 Posilo Makai-1 5.50 4.0 Grand mean 7.11 1.94 21 SO 1 SIWQ-3 11.17 0.0 CV (%) 131.67 266.64 22 S 99 TLYQ-B 19.0 0.83 SD 4.687 2.313 23 Rampur SO 3

FO2 6.33 2.33

LSD (0.05) 10.89 5.827

24 Rampur SO 3 FO4

10.83 2.33

F-test Genotypes (G) * NS

25 Rampur SO 3 FO6

5.17 2.67 Location (L) NA NS

Interaction (G × L) NA NA

NS=Non significant, NA: Not available, * significant at 1% level

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Annexure 32: Response of maize genotypes to maize stem borer, C. partellus Swinhoe. SN Designations Insect score(0-9

scale)*

Percent

insects

damage**

Tunnel

length*

Exit holes*

OPV

1 S99TLYQ-AB 4.0 27.7(5.31) 2.2(1.64) 0.3(0.89)

2 R.POP-2 3.7 26.4(5.19) 2.8(1.82) 0.1(0.77)

3 R.POP-1 2.3 31.6(5.66) 5.1(2.37) 0.7(1.09)

4 EEYC1 2.0 23.6(4.91) 0.7(1.09) 0.8(1.14)

5 ARUN 4 3.0 27.9(5.33) 1.1(1.26) 0.3(0.89)

6 S97TLYGH'A×B'(3) 3.7 26.9(5.23) 0.3(0.89) 0.3(0.89)

7 S97TEYGH'A×B'(3) 3.7 21.6(4.70) 1.3(1.34) 0.6(1.05)

8 POP45/POOL 17 4.7 29.5(5.48) 0.7(1.09) 0.2(0.84)

9 RAMPUR SO3FO4 2.7 24.1(4.96) 3.1(1.90) 0.3(0.89)

10 POP44/POOL 15 2.3 21.6(4.70) 1.8(1.52) 0.3(0.89)

11 ARUN-2 3.3 25.6(5.11) 2.0(1.58) 0.7(1.09)

12 POP 446C1 4.3 40.3(6.39) 1.3(1.34) 0.0(0.71)

13 KHUMAL YELLOW/POOL 17 5.7 40.5(6.40) 2.7(1.79) 0.4(0.95)

14 UPAHAR 3.3 32.0(5.70) 1.8(1.52) 0.5(1.00)

15 ACROSS 9331 3.0 20.9(4.63) 0.1(0.77) 0.2(0.84)

16 POP 445C1 3.3 26.9(5.23) 3.3(1.95) 0.5(1.00)

17 POOL 17 4.3 37.3(6.15) 4.5(2.24) 0.5(1.00)

18 RAMPUR SO3FO2 4.0 42.5(6.55) 2.8(1.82) 0.1(0.77)

19 NARAYANI 2.3 20.3(4.56) 1.4(1.38) 0.1(0.77)

20 O2SAVDIF 2 4.3 31.0(5.61) 0.4(0.95) 0.1(0.77)

21 ACROSS 9942/ACROSS 9944 3.0 21.7(4.71) 3.5(2.00) 0.3(0.89)

22 RAMPUR COMPOSITE 3.0 35.0(5.96) 1.3(1.34) 0.1(0.77)

QPM

23 SOOTLYQ-AB 6.7 39.7(6.34) 1.0(1.22) 0.0(0.71)

24 SOISIWQ-1 4.3 36.9(6.11) 8.6(3.02) 0.1(0.77)

25 SO3TLYQ-AB-2 4.0 21.2(4.66) 4.5(2.24) 0.2(0.84)

26 SO3TLYQ-AB-O1 3.7 32.9(5.78) 0.07(0.75) 0.0(0.71)

27 SOISIWQ-3 3.3 23.1(4.86) 0.00(0.71) 0.1(0.77)

28 SOOTLYQ-B 2.0 29.9(5.51) 0.1(0.77) 0.0(0.71)

Hybrids

29 Rampur composite / RML-8 1.3 14.2(3.83) 8.4(2.98) 0.4(0.95)

30 BIOSEED 3.0 26.0(5.15) 12.2(3.56) 0.5(1.00)

31 RML 4 / NML 2 2.3 20.9(4.63) 0.5(1.00) 0.0(0.71)

32 GAURAV 4.7 34.9(5.95) 1.2(1.30) 0.1(0.77)

F test

CV%

LSD at 0.5 level

*

43.54%

2.472

*

19.92

1.719

**

41.26

1.024

**

18.49

0.269

*Means of three replications.

** Mean of three replications and two observations.

-Figure in the parentheses is the transformed value of original value by Square root of (X

+0.5).