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HLA-G 2015

7th INTERNATIONAL CONFERENCE ON HLA-G

July 6 - 8, 2015

Ministère des Affaires sociales, de la

Santé et des Droits des femmes

PROGRAM

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PRESIDENT Edgardo D. CAROSELLA (France)

INTERNATIONAL SCIENTIFIC ADVISORY BOARD

Laurence AMIOT (France) Ofer MANDELBOIM (Israel) Antonio ARNAIZ-VILLENA (Spain) James McCLUSKEY (Australia) Diego DELGADO (Canada) Fabio MORANDI (Italy) Eduardo A. DONADI (Brazil) José PEÑA-MARTINEZ (Spain) Enrico FAINARDI (Italy) Vera REBMANN (Germany) Soldano FERRONE (USA) Roberta RIZZO (Italy) Álvaro GONZÁLEZ-HERNANDEZ (Spain) Michel ROGER (Canada) Silvia GREGORI (Italy) Barbara SELIGER (Germany) Anatolij HORUZSKO (USA) Nicole SUCIU-FOCA (USA) Thomas HVIID (Denmark) Heinz WIENDL (Germany) Katsumi MAENAKA (Japan) Wei-Hua YAN (P.R.China)

SCIENTIFIC ORGANIZING COMMITTEE

Chairman Edgardo D. CAROSELLA (France)

Co-Chairs François DESGRANDCHAMPS (France) Joël LeMAOULT (France) Philippe MOREAU (France) Nathalie ROUAS-FREISS (France) Diana TRONIK-LE ROUX (France)

Paris, July 6, 2015

Dear Colleagues and Friends, Welcome to Paris where once again for the seventh time since 1998 I organize this international conference dedicated to our research on the HLA-G molecule. I notice with great pleasure that new teams around the world join us to contribute to the progress of our work. Over the last seventeen years we have come a long way from feto-maternal tolerance to oncology, transplantation, autoimmunity and infectious diseases. The HLA-G molecule is today considered a major immune checkpoint in the anti-tumour immune response. Its role in malignant tumours has been widely developed by different teams, over 6000 patients have been studied worldwide and the HLA-G expression is correlated to advanced disease stage or poor prognosis. We did a great job; we still have much to do but the results are very promising, especially in human clinical application. I wish you all a very pleasant and fruitful stay in Paris.

E.D. Carosella

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PLENARY SESSIONS

SPEAKERS

AMIOT L Institute of Research in Environmental and Occupational Health (IRSET), UMR INSERM 1085, University of Rennes 1, France

ARNAIZ-VILLENA A Department of Immunology, The Madrid Regional Blood Center, University Complutense, Madrid, Spain

BORTOLOTTI D Section of Microbiology and Medical Genetics, Department of Medical Sciences, University of Ferrara, Ferrara, Italy

BRUGIÈRE O Pneumology B and Lung Transplantation department, University Hospital Center (CHU) Bichat-Claude Bernard, Paris, France

CAROSELLA ED Hemato-Immunology Research Department (SRHI), CEA Institute of Emerging Diseases and Innovative Therapies (iMETI), Institut Universitaire d’Hématologie, Saint-Louis Hospital, Paris, France

DELGADO D Division of Cardiology and Cardiac Transplantation, University Health Network, Toronto General Hospital, Toronto, Canada

DESGRANDCHAMPS F Department of urology and kidney/pancreas transplantation in Saint-Louis Hospital, Paris, France

DI CRISTOFARO J Geographic immunohematology team of UMR 7268 ADES (Anthropologie bio-culturelle, Droit, Ethique et Santé), French Blood Center - Alpes Méditerranée, Aix-Marseille University-CNRS-EFS, France

DONADI EA Division of Clinical Immunology, Department of Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil

FAINARDI E Neuroradiology Unit, Department of Neurosciences and Rehabilitation, Azienda Ospedaliero-Universitaria of Ferrara, Ferrara, Italy

FERNANDEZ N School of Biological Sciences, University of Essex, Colchester, U.K.

GONZÁLEZ Á Department of Biochemistry, University Clinic of Navarra, Pamplona, Spain

GREGORI S Tolerogenic Dendritic Cells Unit, San Raffaele Institute for Gene Therapy (HSR-TIGET), Milan, Italy

HAN ZC National Engineering Research Center of Stem Cells, Tianjin, P.R. China

HORUZSKO A Cancer Center, Georgia Regents University, Augusta, USA

HVIID TVF Centre for Immune Regulation and Reproductive Immunology (CIRRI), Department of Clinical Biochemistry, Roskilde Hospital, Roskilde, Denmark

KÖNIG L Department of Transfusion Medicine, University Hospital of Essen, Essen, Germany

LeMAOULT J Basic and applied immunobiology laboratory, Hemato-Immunology Research Department (SRHI), CEA Institute of Emerging Diseases and Innovative Therapies (iMETI), Institut Universitaire d’Hématologie, Saint-Louis Hospital, Paris, France

LE PAGE M School of pathology and laboratory medicine, University of Western Australia, Crawley, Australia

MAENAKA K Center for Research and Education for Drug Discovery, Faculty of Pharmaceutical Sciences, Hokkaido University, Hokkaido, Japan

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MANDELBOIM O Immunology and cancer research, Institute for Medical Research Israel-Canada (IMRIC), The Hebrew University, Jerusalem, Israel

MORANDI F Laboratory of Oncology, Giannina Gaslini Institute, Genoa, Italy

MOREAU P Regulation of Expression of Immune Response Genes team, Hemato-Immunology Research Department (SRHI), CEA Institute of Emerging Diseases and Innovative Therapies (iMETI), Institut Universitaire d’Hématologie, Saint-Louis Hospital, Paris, France

PANKRATZ S Department of Neurology, University Hospital Münster, Münster, Germany

REBMANN V Department of Transfusion Medicine, University Hospital of Essen, Essen, Germany

RIZZO R Section of Microbiology and Medical Genetics, Department of Medical Sciences, University of Ferrara, Ferrara, Italy

ROGER M Molecular biology laboratory, Centre Hospitalier de l’Université de Montréal, Hôpital Notre-Dame, Montreal, Canada

ROUAS-FREISS N Laboratory of Transplantation Immunology, Hemato-Immunology Research Department (SRHI), CEA Institute of Emerging Diseases and Innovative Therapies (iMETI), Institut Universitaire d’Hématologie, Saint-Louis Hospital, Paris, France

SELIGER B Institute for Medical Immunology, Martin-Luther University Halle-Wittenberg, Halle Saale, Germany

SIGAUX F French National Cancer Institute (INCa), Cancer Multi-organization thematic institutes of the French National Alliance for Life Sciences and Health (ITMO Cancer – Aviesan), Paris, France

WIENDL H Department of Neurology, University of Münster, Münster, Germany

YAN WH Medical Research Center, Taizhou Hospital of Zhejiang province, Linhai, Zhejiang, P.R. China

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SYMPOSIA SPEAKERS

d'ALMEIDA T Université Pierre et Marie Curie, Paris VI; UMR 216-MERIT, Institut de Recherche pour le Développement; Faculté de Pharmacie - Université Paris Descartes, Sorbonne Paris-Cité, Paris, France

AMODIO G San Raffaele Telethon Institute for Gene Therapy (TIGET), Division of Regenerative Medicine, Stem Cells and Gene Therapy, IRCCS San Raffaele Scientific Institute, Milan; Vita-Salute San Raffaele University, Milan, Italy

CZARNECKI C Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba ; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada

DA SILVA NARDI F Institute for Transfusion Medicine, University Hospital Essen, Essen, Germany; Laboratory of Immunogenetics and Histocompatibility (LIGH), Federal University of Paraná, Curitiba, Brazil

GRAMIGNOLI R Karolinska Institute, Department of Laboratory Medicine, Stockholm, Sweden

HAGHI M Department of Genetics, Faculty of Biological Sciences, Tarbiat Modarres University, Tehran; Department of Genetics, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

HORUZSKO A Cancer Center, Georgia Regents University, Augusta, USA

KANGA U Department of Transplant Immunology and Immunogenetics, All India Institute of Medical Sciences, New Delhi, India

LAZARTE J Division of Cardiovascular Surgery, Toronto General Hospital, University Health Network, University of Toronto, Canada

LÉGER L UMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Biopôle, Université de Lorraine Nancy, France

NASCIMENTO WGA Center for Immune Regulation and Reproductive Immunology (CIRRI), Copenhagen University Hospital (Roskilde), Køgevej 7-13, 4000 Roskilde, Denmark

PANCOTO JAT Department of Health Sciences, UFES, Brazil

PARK Y Department of Laboratory Medicine, Catholic Kwandong University College of Medicine, Incheon, Korea

PUPPO F Clinical Immunology Unit, Department of Internal Medicine, University of Genoa, Genoa, Italy

QUACH K CReATe Fertility Centre, Toronto, Canada

SAYED D Flow Cytometry Lab, Department of Clinical pathology, South Egypt Cancer Institute, Assiut University, Egypt

SENEBY L Department of Surgery and of Immunohematology, Leiden University Medical Center, Leiden, The Netherlands

SGORLA B Universidade Federal de Santa Catarina, Florianópolis, Brazil

STAMOU P Bone Marrow Transplantation laboratory, Department of Medicine, University of Patras, Greece

SWETS M Department of Surgery and Oncology, Leiden University Medical Centre, Leiden, The Netherlands

TAKAHASHI A Graduate School of Pharmaceutical Science, Hokkaido University, Sapporo, Japan

TRONIK-LE ROUX D High-throughput analysis team, Hemato-Immunology Research Department (SRHI), CEA Institute of Emerging Diseases and Innovative Therapies (iMETI), Institut Universitaire d’Hématologie, Saint-Louis Hospital, Paris, France

VEIT TD Laboratório de Imunogenética, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

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POSTER SESSIONS

ABBAS A Department of Biological Sciences, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND, USA

AFKHAMI F Department of Medical Genetic, Medical faculty; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

BORTOLOTTI D Section of Microbiology and Medical Genetics, Department of Medical Sciences, University of Ferrara, Ferrara, Italy

CARLINI F Aix-Marseille Université, CNRS, EFS, ADES UMR 7268, Marseille, France

CHIES JAB Laboratory of Immunogenetics, Department of Genetics, Federal University of Rio Grande do Sul, Porto Alegre, Brazil

CRISPIM-FREITAS J Department of Clinical Analysis, Federal University of Rio Grande do Norte, Natal, Brazil

GINEAU L Institut de Recherche pour le Développement, UMR216 MERIT, Mère et enfant face aux infections tropicales, Paris; Faculté de Pharmacie, Université Paris Descartes, Sorbonne Paris Cité, Paris, France

KANGA U Department of Transplant Immunology and Immunogenetics, All India Institute of Medical Sciences, New Delhi, India

KÖNIG L Department of Gynecology and Obstetrics; Institute for Transfusion Medicine, University of Duisburg-Essen, Essen, Germany

LAZARTE J Division of Cardiovascular Surgery, Toronto General Hospital, University Health Network, University of Toronto, Canada

LUCENA-SILVA N Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz - FIOCRUZ, Recife; Oncologia Pediátrica, Instituto de Medicina Integral Professor Fernando Figueira, Recife, Brazil

LYNGE-NILSSON L Centre for Immune Regulation and Reproductive Immunology (CIRRI), Dept. of Clinical Biochemistry, Copenhagen University Hospital (Roskilde), Denmark

MITRA DK Department of Transplant Immunology and Immunogenetics, All India Institute of Medical Sciences, New Delhi, India

MOHANTY S Stem Cell Facility, DBT-Center for Excellence for Stem Cell Research, All India Institute of Medical Science, New Delhi, India

MUNIZ YCN Universidade Federal de Santa Catarina, Florianópolis, Brazil

RIZZO R Section of Microbiology and Medical Genetics, Department of Medical Sciences, University of Ferrara, Ferrara, Italy

SÁEZ GUTIÉRREZ B Instituto Investigación Aragon, Laboratorio de Inmunología y Cáncer, Hospital Clínico universitario "Lozano Blesa", Zaragoza, Spain

SBARSI I SIMT, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy

SIMÕES RT Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte, Minas Gerais, Brazil

SVENDSEN SG Department of Immunology and Microbiology, University of Copenhagen, Denmark

WAGNER B Institute for Transfusion Medicine, University Hospital Essen, Essen, Germany

WASTOWSKI IJ Department of Biology, State University of Goiás, Morrinhos, Goiás, Brazil

YAN WH Medical Research Center, Taizhou Hospital of Zhejiang Province, Linhai, Zhejiang, P.R. China

ZAMBRA FMB Department of Genetics, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil

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JULY 6, 2015

PLENARY SESSION 1

HLA-G Immune Functions Chairmen: Edgardo D. CAROSELLA, Ofer MANDELBOIM

08:30 REGISTRATION 9:30 - 11:45 _______________________________________________________________________________________________ 09:30 Opening Address and Tribute to Pr. Hans Grosse-Wilde E. D. CAROSELLA (France) _______________________________________________________________________________________________ 09:40 New insights on the immune modulatory activity of DC-10 S. GREGORI (Italy) _______________________________________________________________________________________________ 10:05 The immune regulatory role of human HLA-G expressing S. PANKRATZ (Germany)

Regulatory CD4+ T cells in vivo _______________________________________________________________________________________________ 10:30 KIR2DL4 and controversial activity in HLA-G responses M. LE PAGE (Australia) _______________________________________________________________________________________________ 10:55 The odd relationship between HLA-G function and J. LeMAOULT (France)

ILT2 expression _______________________________________________________________________________________________ 11:20 Experimental models for study function of HLA-G in vivo A. HORUZSKO (USA) _______________________________________________________________________________________________

COFFEE BREAK 11:45 - 12:00 _______________________________________________________________________________________________

SYMPOSIUM I

Chairpersons: Diego DELGADO, Nathalie ROUAS-FREISS 12:00 - 13:30 _______________________________________________________________________________________________ Hypomethylating Agents Induce HLA-G+ Immunoregulatory T Cells in vitro Panagiota Stamou, Dimitra Marioli, Vasiliki Goudogianni, Efthymia Theofani, Argyro Sgourou, Chryso Pierides, Paul A. Costeas, Panagiotis Zikos, Alexandros Spyridonidis _______________________________________________________________________________________________ HLA-G Shapes the Ability of DC-10 to Promote T Regulatory Type 1 (TR1) Cells Giada Amodio, Michela Comi, Daniela Tomasoni, Monica Emma Gianolini, Roberta Rizzo, Joël LeMaoult, Maria-Grazia Roncarolo, and Silvia Gregori _______________________________________________________________________________________________ Investigation of Regulatory T Cells in co-Culture with the JEG-3 Cell Line: a Role for HLA-G? Nascimento W.G.A., Andersen M.H. and Hviid T.V.F. _______________________________________________________________________________________________ Mechanisms Reprogramming B Cell by HLA-G and their Signature in Kidney Transplant Patients Maureen Ezeakile, Vera Portik-Dobos, Daniel D. Horuzsko, Rajan Kapoor, Katsumi Maenaka, Carlos F. Zayas, Laura L. Mulloy, Anatolij Horuzsko _______________________________________________________________________________________________

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______________________________________________________________________________________________ Evaluation of Soluble HLA-G Antigens in Systemic Sclerosis Paola Contini, Matteo Borro, Simone Negrini, Francesca Spanò, Giuseppe Murdaca, Francesco Puppo _______________________________________________________________________________________________ HLA-G in Neutrophils of SLE Patients: Correlation with Adhesion Molecules and Apoptosis Markers João a. T. Pancoto, Everton o. L. Santos, Yara m. Lucisano-valim, Marcelo Dias-Baruffi, Eduardo A. Donadi _______________________________________________________________________________________________

LUNCH BREAK 13:30 - 14:30 _______________________________________________________________________________________________

PLENARY SESSION 2

HLA-G in Autoimmunity Chairwoman: Roberta RIZZO

14:30 - 14:45 _______________________________________________________________________________________________ 14:30 HLA-G in the nervous system: state of knowledge H. WIENDL (Germany) _______________________________________________________________________________________________ 14:55 HLA-G in different brain disorders including MS E. FAINARDI (Italy) _______________________________________________________________________________________________ 15:20 HLA-G in diabetes E.A. DONADI (Brazil) _______________________________________________________________________________________________

COFFEE BREAK 15:45 - 16:00 _______________________________________________________________________________________________

SYMPOSIUM II

Chairmen: Joël LeMAOULT, Wei-Hua YAN 16:00 - 18:00 _______________________________________________________________________________________________ Human Leukocyte Antigen-G Polymorphisms as Predictors of Cardiac Allograft Vasculopathy Julieta Lazarte, Livia Goldraich, Hiroyuki Kawajiri, Arash Ghashghai, Liza Grosman-Rimon, Vivek Rao, Diego Delgado _______________________________________________________________________________________________ HLA-G 3’ UTR Polymorphism Regulates HLA-G Expression: Immunomodulation in HSCT Uma Kanga, Manish Mourya, Tulika Seth, Manoranjan Mahapatra, Poonam Coshcic _____________________________________________________________________________________________ Soluble HLA-G in Synovial Fluid from Patients with Rheumatoid Arthritis and Juvenile Idiopathic Arthritis Tiago Degani Veit, Vera Rebmann, Bettina Wagner, Peter Horn, Claiton Viegas Brenol, João Carlos Tavares Brenol, Ilóite Maria Scheibel, Markus Bredemeier, Ricardo Machado Xavier, José Artur Bogo Chies _____________________________________________________________________________________________ The Immunosuppressive Effect of HLA-G2/G6 Homodimer in Collagen-Induced Arthritis Mice Ami Takahashi, Kimiko Kuroki, Katsumi Maenaka _____________________________________________________________________________________________ Cellular Barcoding Strategy for Simultaneous Clonal Analysis of Tumour Cells Diana Tronik-Le Roux, Julie Renard, Aurore Poux, Edgardo D. Carosella _____________________________________________________________________________________________

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_____________________________________________________________________________________________ Association of HLA-G Polymorphisms and Psoriasis Bibiana Sgorla de Almeida, Renata Toscano Simões, Sandra Regina Rachadel Torres, Daniel Holthausen Nunes, Andrea Rita Marrero, Ilíada Rainha de Souza, Yara Costa Netto Muniz _____________________________________________________________________________________________

POSTER SESSION I

09:30 - 18:00

1. HLA-G and CD83 Expressing DC-10 Accumulate in Patients with Gastric Cancer Dan-Ping Xu, Aifen Lin, Wei-Hua Yan

2. The Prognostic Impact of Soluble HLA-G and its Relationship to Circulating Tumour Cells in Neoadjuvant

Treated Breast Cancer Patients Lisa König, Sabine Kasimir-Bauer, Oliver Hoffmann, Ann-Kathrin Bittner, Sabine Schramm, Agnes Bankfalvi, Rainer Kimmig, Peter A. Horn, Vera Rebmann

3. Potential Role for Omega-3 Fatty Acid in the Inhibition of Breast Cancer Metastasis Mediated by Modulation

of Human Leukocyte Antigen-G (HLA-G) Expression Ata Abbas, Humaira Qureshi, Nii K-K. Koney, Niraj Nepal, Sergei Nechaev, Archana Dhasarathy, Philippe T. Georgel

4. Lack of Association of the Polymorphisms HLA-G and IL-17A in Tacrolimus-Treated Renal Transplanted

Patients Antonnyo Palmielly Diógenes Lima, Luan Fonseca, Julieta Genre, Kleyton Thiago Costa de Carvalho, Renata Gomes Alves, Francisco Pignataro Lima, Luiz Reginaldo Menezes da Rocha, Maurício Galvão Pereira, Janaina Cristiana de Oliveira Crispim Freitas

5. Comparative Analysis of HLA-G Expression in Mesenchymal Stem Cells Obtained from Various Tissues

Sujata Mohanty, Pallavi Srivastava, Punit Prabha, Manisha Singh, Anupama Kakkar, V.Seenu, O.P.Kharbanda, Balram Airan

6. The HLA-G 14-bp Insertion/Deletion Polymorphism in Type 1 Diabetes Patients

Janaina C. O. C. Freitas, Heglayne P. V. Silva, Marcela A. G. Ururahy, Karla S. C. Souza, Melina B. Loureiro, Yonara M. C. Oliveira, Gustavo H. M. Oliveira, André D. Luchessi, Kleyton T. C. Carvalho, Eduardo A. Donadi, Rosario D. C. Hirata, Maria das G. Almeida, Ricardo F. Arrais, Mario H. Hirata, Adriana A. Rezende

7. Differential Expression of Soluble and Cell Surface HLA-G among Tuberculosis Patients

DK Mitra, Abhinav Saurabh, Deepshi Thakral, Manish Mourya, Anant Mohan, Uma Kanga 8. Down Regulation of HLA-G Receptor (ILT2) Expression at the Local Disease Site in Tuberculous Patients

Uma Kanga, Abhinav Saurabh, Amar Singh, Deepshi Thakral, Anant Mohan, DK Mitra 9. Expression of HLA-G in the Retinal Pigment Epithelial Cells and its Importance for the Development of Age-

Related MacularDegeneration Signe Goul Svendsen, Marina Daouya, Ching-Lien Wu, Carsten Faber, Mads Krüger Falk, Amardeep Singh, Torben Lykke Sørensen, Thomas Vauvert Faurschou Hviid, Edgardo D. Carosella, Joël LeMaoult, Mogens Holst Nissen

10. Expression of HLA-G Molecule in Lip Carcinogenesis

Andréia Souza Gonçalves, Jéssica Petini de Oliveira, Elaine Nayara Gomes Santana, Tarcília Aparecida Silva, Elismauro Francisco Mendonça, Isabela Jubé Wastowski, Aline Carvalho Batista

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11. Correlation of HLA-G, IL-10 and TNF Gene Expression with the Degree of Intestinal Lesion in Biopsies of Brazilian Patients with Inflammatory Bowel Disease Renan Garcia Gomes, Carlos Alexandre de Brito, Valéria Martinelli, Laís Baracho da Costa, Eduardo Antônio Donadi, Norma Lucena-Silva

12. The HLA-G 14-bp Insertion/Deletion Polymorphism in Recurrent Spontaneous Abortion among Iranian

Women Fatemeh Afkhami, Mahmoud Shekari Khaniani, Layia Farzadi, Zeynab Paknejad, Sima Mansoori Derakhshan

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JULY 7, 2015

PLENARY SESSION 3

HLA-G in Cancer Chairmen: François DESGRANDCHAMPS, Soldano FERRONE

9:00 - 11:05 _______________________________________________________________________________________________ 09:00 HLA-G dependent tumour development B. SELIGER (Germany) _______________________________________________________________________________________________ 09:25 HLA-G / ILT4 in bladder cancer F. DESGRANDCHAMPS (France) _______________________________________________________________________________________________ 09:50 HLA-G in malignant lesions W.H. YAN (P.R. China) _______________________________________________________________________________________________ 10:15 HLA-G in normal and neoplastic B-cells N. ROUAS-FREISS (France) _______________________________________________________________________________________________ 10:40 Checkpoints in anti-tumour immune response F. SIGAUX (France) _______________________________________________________________________________________________

COFFEE BREAK 11:05 - 11:20 _______________________________________________________________________________________________

PLENARY SESSION 4

HLA-G and Infectious Diseases Chairman: José PEÑA-MARTINEZ

11:20 - 13:00 _______________________________________________________________________________________________ 11:20 HLA-G molecules and microbial infections R. RIZZO (Italy) _______________________________________________________________________________________________ 11:45 HLA-G in human liver diseases L. AMIOT (France) _______________________________________________________________________________________________ 12:10 Natural immunity/resistance to HIV-1 infection is associated with a M. ROGER (Canada)

genital mucosal HLA-G tolerogenic/regulatory microenvironment _______________________________________________________________________________________________ 12:35 Clinical and functional aspects of HLA-G bearing extracellular L. KÖNIG (Germany)

vesicles in tumour context _______________________________________________________________________________________________

LUNCH BREAK 13:00 - 14:00 _______________________________________________________________________________________________

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PLENARY SESSION 5

HLA-G Gene Expression and Structure Chairwomen: Barbara SELIGER, Nicole SUCIU-FOCA

14:00 - 15:40 _______________________________________________________________________________________________ 14:00 Structural aspects of HLA-G isoforms and receptor binding K. MAENAKA (Japan) _______________________________________________________________________________________________ 14:25 Structures of circulating HLA-G. Methodological consideration A. GONZÁLEZ (Spain)

for HLA-G measurement _______________________________________________________________________________________________ 14:50 Control of classical and non-classical MHC class I expression O. MANDELBOIM (Israel)

by RNA binding proteins _______________________________________________________________________________________________ 15:15 HLA-G gene regulation of expression through its 3’UTR P. MOREAU (France) _______________________________________________________________________________________________

COFFEE BREAK 15:40 - 16:00 _______________________________________________________________________________________________

SYMPOSIUM III

Chairmen: Antonio ARNAIZ-VILLENA, Philippe MOREAU 16:00 - 17:00 _______________________________________________________________________________________________ HLA-G Expression in Primary Colorectal Cancer and Associated Liver Metastases Marloes Swets, Liz Lieverse, Lina Seneby, Hans Gelderblom, Gerrit Jan Liefers, Cornelis J.H. van de Velde, Peter J.K. Kuppen ______________________________________________________________________________________________ Promoter Methylation and HLA-G Expression in Colon Cancer Lina Seneby, Marloes Swets, Cornelis JH van de Velde, Peter J. van den Elsen, Peter J.K. Kuppen _______________________________________________________________________________________________ MDR-1 Expression and Soluble HLA-G Levels in B-Chronic Lymphocytic Leukemia: an Impact on Prognosis Douaa Sayed, Abeer Ibrahim, Alia M. A. Attia, Amira H. Soliman _______________________________________________________________________________________________ Increment of HLA-G Expression are Affected by the HLA-G 14-bp Polymorphism in Acute Leukemia Younhee Park, Hae Jin Kim, Hyon-Suk Kim _______________________________________________________________________________________________ 14-bp Insertion/Deletion Polymorphism of HLA-G Gene and Progression of Breast Cancer Mehdi Haghi, Mohammad Ali Hosseinpour Feizi, Majid Sadeghizadeh, Abbas Sahebghadam Lotfi _______________________________________________________________________________________________

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POSTER SESSION II

09:00 - 17:00

1. Elevated Levels of Soluble HLA-E and the Presence of HLA-E*01:03 Predict Disease Progression in CLL

Bettina Wagner, Fabiola da Silva Nardi, Sabine Schramm, Thomas Kraemer, Christina Bade-Döding, Jan Dürig, Peter A. Horn, Ulrich Dührsen, Holger Nückel, Vera Rebmann

2. UTR-4 Haplotype and +3003C Polymorphism of the HLA-G Gene as Risk Factors to Prostate Cancer

Francis Maria Báo Zambra, Vanderlei Biolchi, Caio Cesar Silva de Cerqueira, Ilma Simoni Brum da Silva, Erick da Cruz Castelli, José Artur Bogo Chies

3. The Expression of HLA-G and HLA-E in Oral Squamous Cell Carcinoma

Millena Carolina Pereira Silva Sousa, Isabela Jubé Wastowski, Kênia Cristina Soares Fonseca de Magalhães, Fabiana Alves Miranda de Camargo, Ênio Ferreira, Istéfani Luciene Dayse da Silva, Edson Garcia Soares, Renata Toscano Simões

4. Differences on the HLA-G 3’UTR Genetic Variability and Bone Marrow sHLA-G Levels at Diagnosis and

after Induction Therapy in Childhood Acute Leukemia Renata Dos Santos Almeida, Alessandra Maria De Luna Ramos, Ester Augusta Vinhas Marques, Francisco Pedrosa, Eduardo Antônio Donadi, Norma Lucena-Silva

5. The +3003 TT and +3035 TT Genotypes are Associated with High Risk of Childhood Burkitt’s Lymphoma

Márcia F. Pedrosa, Laís Baracho, Renata S. Almeida, Francisco Pedrosa, Eduardo A. Donadi, Rocio Hassan, Norma Lucena-Silva

6. High Tissue Expression of HLA-G by Homozygote Haplotypes of 14-bp / +3142 Polymorphisms in Patients

with Oral Cancer Millena Carolina Pereira Silva Sousa, Kênia Cristina Soares Fonseca de Magalhães, Isabela Jubé Wastowski, Fabiana Alves Miranda de Camargo, Istéfani Luciene Dayse da Silva, Edson Garcia Soares, Renata Toscano Simões

7. HLA-G and E-Cadherin Expression and its Prognostic Value in Renal Cell Carcinoma Sáez Gutiérrez B., Tobeña Puyal M., Segura Gil I., Gimeno Pelegrin J., Sota Ochoa P., Segura I., Navarro Gil J., Capapé Poves V., Lambea Sorrosal JJ., Sánchez Zalabardo JM., Larrad Mur L., Tres Sánchez A.

8. Significance of Lesion HLA-G5/-G6 Isoform Expression in Ovarian and Lung Cancer Patients Aifen Lin, Xia Zhang, Wei-Hua Yan

9. Association of Soluble HLA-G Levels and Immunosuppressive Treatment

Julieta Lazarte, Laura Tumiati, Hiroyuki Kawajiri, Arash Ghashghai, Liza Grosman-Rimon, Vivek Rao, Diego Delgado

19:00 – 21:00 COCKTAIL

(by invitation only)

HENJO Houseboat (55 quai de la Tournelle 75005 Paris)

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JULY 8, 2015

PLENARY SESSION 6

HLA-G in Transplantation Chairmen: Christophe HENNEQUIN, Anatolij HORUZSKO

9:00 - 11:30 _______________________________________________________________________________________________ 09:00 New insights on HLA-G and vascular diseases D. DELGADO (Canada) _______________________________________________________________________________________________ 09:25 Roles of mesenchymal stem cells in immunomodulation and Z.C. HAN (P.R. China)

tumour growth _______________________________________________________________________________________________ 09:50 HLA-G and HLA-E in transplantation V. REBMANN (Germany) _______________________________________________________________________________________________ 10:15 Interactions between HLA-G, HLA-E and HLA-F in physiological F. MORANDI (Italy)

and pathological conditions _______________________________________________________________________________________________ 10:40 Role of HLA-G as a predictive marker of low risk of chronic O. BRUGIÈRE (France)

rejection in lung transplant recipients _______________________________________________________________________________________________ 11:05 HLA-G genetic polymorphisms and lung transplantation J. DI CRISTOFARO (France) _______________________________________________________________________________________________

COFFEE BREAK 11:30 - 11:45 _______________________________________________________________________________________________

SYMPOSIUM

IV Chairpersons: Laurence AMIOT, Diana TRONIK-LE ROUX

11:45 - 13:15 _______________________________________________________________________________________________ KIR2DL4 Polymorphism and Epistatic Interactions with HLA-G in Perinatal HIV-1 Transmission Chris Czarnecki, Emma Rossnagel, Sue Ramdahin, Joanne Embree, Francis Plummer, Ma Luo _______________________________________________________________________________________________ A Combination of Single Nucleotide Polymorphisms in the 3’Untranslated Region of HLA-G Reduces HLA-G Expression and is Associated with Preeclampsia Quach K, Behrouzi B, Hahn K, and Librach _______________________________________________________________________________________________ Differential Expression Profile of Free HLA-G and Extracellular Vesicles Bound Molecules in Implantation Failure Patients and Pregnant Women Fabiola da Silva Nardi, Geórgia Fernanda Gelmini, Renata Slowik, Tatiana Ferreira Michelon, Cynthia Hernandes Costa, Jorge Milton Neumann, Peter A. Horn, Maria da Graça Bicalho, Vera Rebmann _______________________________________________________________________________________________ Soluble HLA-G Levels during Pregnancy and Infancy: Mother-Child Resemblance Tania d’Almeida, Ibrahim Sadissou, Jacqueline Milet, Gilles Cottrell, Philippe Moreau, Benoit Favier, Kabirou Moutairou, ED Carosella, Nathalie Rouas-Freiss, André Garcia, David Courtin _______________________________________________________________________________________________

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_______________________________________________________________________________________________ HLA-G Expression in Human Amnion Epithelial Cells and their Therapeutic Use to Correct Acute Liver Failure and Metabolic Liver Disease Roberto Gramignoli, Stephen C Strom _______________________________________________________________________________________________ Evolution of HLA-G Expression by Wharton’s Jelly’s Mesenchymal Stromal Cells during Chondrogenic Differentiation in two Different Culture Methods Léonore Léger, Loïc Reppel, Céline Huselstein, Danièle Bensoussan

_______________________________________________________________________________________________

_________LUNCH BREAK 13:15 - 14:15 _______________________________________________________________________________________________

PLENARY SESSION 7

HLA-G Polymorphism and Pregnancy Chairmen: Álvaro GONZÁLEZ HERNÁNDEZ, Vito PISTOIA

14:15 - 15:35 _______________________________________________________________________________________________ 14:15 HLA-G in reproduction T. V. HVIID (Denmark) _______________________________________________________________________________________________ 14:35 Significance of differential null HLA-G alleles distribution in world A. ARNAIZ-VILLENA (Spain) _______________________________________________________________________________________________ 14:55 HLA-G and other HLA Class I subsets on human embryos: N. FERNANDEZ (U.K.)

development of methods to quantify co-localisation _______________________________________________________________________________________________ 15:15 HLA-G, infertility and congenital infections D. BORTOLOTTI (Italy) _______________________________________________________________________________________________________

15:55 CLOSING REMARKS

Silvia GREGORI _________________________________________________________________________________________________________

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POSTER SESSION III

09:00 - 15:55 1. Association of Asthma Pathogenesis with HLA-G Genetic Polymorphisms and Differential HLA-G Isoforms

Expression: a Central Role of Human Bronchial Epithelial Cells Federico Carlini, Céline Garulli, Pierre Roubertoux, Christophe Picard, Pascal Chanez, Delphine Gras, Julie Di Cristofaro

2. Association between Soluble HLA-G Level and Stages of Human African Trypanosomiasis

Gineau Laure, Courtin David, Jamonneau Vincent, Dias Fabricio, Tokplonou Leonidas, Ilboudo Hamidou, Castelli Eric, Milet Jacqueline, Mamadou Camara, Rouas-Freiss Nathalie, Moreau Philippe, Donadi Eduardo A, Bucheton Bruno, Sabbagh Audrey, Garcia André

3. rs1233334 (HLA-G -725 G/C/A) and rs1063320 (HLA-G +3142 C/G) Seem not to Be Associated with sHLA-G

Levels in Cord Blood Units (CBU) I. Sbarsi, P. Bergamaschi, S. Ferrari, M. Zorzetto, A. Cremaschi, V. Genovese, I. Ferrarotti, C. Tinelli, M. Guarene, B. Romano, C. Badulli, C. Monti, C. Pizzochero, A. Pasi, L. Salvaneschi

4. Distribution of HLA-G Extended Haplotypes and One Non-Synonymous HLA-E Allele in a Large-Scale

Study of Offspring Cases of Severe Preeclampsia Line Lynge Nilsson, Snezana Djurisic, Anne-Marie Nybo Andersen, Mads Melbye, Ditte Bjerre, Laura Ferrero-Miliani, Daniel E. Geraghty and Thomas Vauvert F. Hviid

5. Polymorphisms Analysis of the 3 'Untranslated Region HLA-G Gene in Santa Catarina Patients with History

of Rheumatoid Arthritis and Breast Cancer Leili Daiane Hausmann, Gabriela Duarte Karasiak, Bibiana Sgorla de Almeida, Sandra Regina Rachadel Torres, Andrea Rita Marrero, Ilíada Rainha de Souza, Yara Costa Netto Muniz

6. HLA-G 3’UTR Haplotypes in Women with Recurrent Pregnancy Losses: a Case-Control Study

Rafael Tomoya Michita, Francis Maria Báo Zambra, Lucas Rosa Fraga, Maria Teresa Sanseverino, Priscila Vianna and José Artur Bogo Chies

7. Heart Transplantation and Microbial Infections: Soluble HLA-G and CD80 Molecule Expression

Roberta Rizzo, Daria Bortolotti, Luciano Potena, Laura Borgese, Valentina Manfredini, Evangelia Petrisli, Marco Masetti, Niccolò Gazzieri, Valentina Gentili, Liliana Gabrielli, Antonella Rotola, Tiziana Lazzarotto, Dario Di Luca

8. Elevation of HLA-G+ MDSCS in Patients with Chronic HBV Infection

Hai-Yan Lu, Aifen Lin, Wei-Hua Yan 9. Soluble HLA-G in Congenital Human Cytomegalovirus Infection

Daria Bortolotti, Valentina Gentili, Tiziana Lazzarotto, Liliana Gabrielli, Silvia Bolzani, Brunella Guerra, Maria Grazia Capretti, Maria Paola Landini, Dario Di Luca, Roberta Rizzo

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GENERAL INFORMATION

Location

Ministère des Affaires sociales, de la Santé et des Droits des femmes 14 avenue Duquesne

75007 Paris

Ecole Militaire

Saint-François Xavier Ségur

85 avenue Bosquet

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COCKTAIL : July the 7th

(by invitation only)

HENJO Houseboat (55 quai de la Tournelle – under the Archevêché bridge)

Maubert Mutualité

Pont Marie

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SPONSORS

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