Present Vidas

8/13/2019 Present Vidas

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vidas system10/05/2005 - p 1

bioMrieux

ImmunoassaySystems


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V ITEKI MMUNO

DIAGNOSTIC

A SSAYS YSTEM

- 1-1


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VIDAS - The Concept


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SPR coated with antibodies

or antigens, serving also as

pipeting device

strip containing all the

ready-to-use reagents

UNIQUE and original

unitaryconcept, avoidingany cross contamination.

1 SPR + 1 strip = 1 patient

VIDAS - Disposables


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VIDAS : an easy loading


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VIDAS : a dynamic process

Strip

SPR

Time & delays Movements Volumes

}depend on the PROTOCOL


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Photodiode

Washingbuffers Substrate

Sample

Diluent

(Pre-wash) Conjugate

Example of a reaction


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ECHANTILLON TAMPON CONJUGUE SUBSTRAT

DE LAVAGE

E

E

E

E

E

E

E

Antigen detection : example of procedure

Cycledin and out

SPR

Target

organism

sample washing conjugate substratebuffer

IMMUNOLOGICAL REACTION ENZYMATIC REACTION


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Principle for an antigen detection test

sandwich principle

Bound Ab

Ag to be detected

Alkaline phosphatase-

labeled Ab

4-methyl umbelliferyl phosphate


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Filters

Lenses

PhotodiodeXenonFlashLamp

VIDAS : reading in fluorescence


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labeled hapten

Hapten to be detected

Bound Ab

COMPETITION

Detection of haptens



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labeled Ab

Ab to be

detected

Bound Ag

ELISA INDIRECT

Serologies


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Ab to be

detected

Ag in liquid medium

Anti-IgM

IMMUNOCAPTURE

Detection of specific IgM


alkaline phosphatase-

labeled Ab


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A memorized calibration


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Calibration (of quantitative tests)

1 - The Master Curve (reference curve)

is performed by QC Lab

0

1000

2000

3000

4000

5000

6000

0 6 12 18 24 30 36 42 48 54

R

FV

Concentration

+++ ++++

++++++++

++++++

++++

++++

+++++

+++++


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1 - Results are summarized on a card

07714_A30406

VIDAS

LH

1 AQ8TE5K2 J21QY3Y

3 6VO6HJ6

4 N65CHD

5 JFK355

6 4

7 4G54

8

9

10

11

12

13

14 15

16 C

Assay:LH

Striplot:030929-0

StandardR

FVValues:

149,635,2215,5061

Calibration

method:

7

Calibration

(S1)DoseValue:

48.2mUI/ml

ControlC1

DoseValueRange:

5.27

--

7.37mUI/ml

Calibrator

(S1)RFVrange:

1858--3318

Max.CV%:

10.0

bioMrieu

xsa

69280

Marc

yl'EtoileFrance


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MLE card description

2 The MLE is to be read by VIDAS


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2

The MLE is to be read by VIDAS,to store the master curve

0 7 714

_A

30406 VIDAS LH

1

AQ8TE5K

2

J21QY3Y

3

6VO6HJ6

4

N65CHD

5

JFK355

6

4

7

4G54

8

9

10

11

12

13

14

15

16

C

Assay : LH Strip lot : 030929-0

S ta nd ard RF V Va lu es : 1 49 , 63 5, 22 15 ,5 06 1

Calibration method : 7

Calibration(S1) Dose Value : 48.2 mUI/ml

Control C1 Dose Value Range : 5.27 -- 7.37mUI/ml

Calibrator (S1) RFV range : 1858--3318 Max. CV%:10.0

bioMrieux sa 69280 Marcy l'Etoile France

0 7 714

_A

30406 VIDAS LH

1

AQ8TE5K

2

J21QY3Y

3

6VO6HJ6

4

N65CHD

5

JFK355

6

4

7

4G54

8

9

10

11

12

13

14

15

16

C

Assay : LH Strip lot : 030929-0

S ta nd ard RF V Va lu es : 1 49 , 63 5, 22 15 ,5 06 1

Calibration method : 7

Calibration(S1) Dose Value : 48.2 mUI/ml

Control C1 Dose Value Range : 5.27 -- 7.37mUI/ml

Calibrator (S1) RFV range : 1858--3318 Max. CV%:10.0

bioMrieux sa 69280 Marcy l'Etoile France

The theoretical calibration

curve is stored in memory


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Calibration When to perform what ?

New Lot of Reagent :1. MLE card2. Adjustment with S1 (or S2)

Every 14 Days :

1. Adjustment with S1 (or S2)


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THE CURRENT VIDAS MENU

Immunochemistry

Infectious diseases

Immunocoagulation

Antigen detection

Industry


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VIDAS : more than 90 parameters

Infectious diseases

HIV : early screening and confirmation: HIV duo ,Ag P24...

Hepatitis diagnosis : HAV IgM, Ag HBs, HBc IgM, anti HBs...

Toxoplasmosis, Rubella , CMV...and confirmation withavidity

Antigen detection : Chlamydia, clostridium difficile toxin A...

Immunochemistry

Fertility : hCG, Estradiol, FSH, LH, Prolactine, Progesteron, Testo..

Thyroid :TSH, T3, T4...

Tumor markers : ACE, AFP, TPSA, CA...

Allergy : IgE, Stallertest, Stallertroph, Stallergy

Immunocoagulation

exclusion of deep venous thrombosis :D Dimer


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VIDAS and mini VIDAS :

2nd immunoassay system

sold in the world

Documents

Present Vidas