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- Perform a first-hand investigation to model the selectively permeable nature of a cell membrane Beakers A and B The dialysis tubing has small pores which allow certain substances of appropriate size to pass through. Allows iodine and water to pass through but stops large starch molecules Analysis Increase of mass of bag in BEAKER A indicates osmosis occurring, through tubing into the bag (WATER) Colour of Iodine: BROWN —> BLUE-BLACK by presence of starch BLUE-BLACK colour of bag indicates that iodine has diffused through the tubing and since the Iodine solution in the beaker did not change, no starch diffused out In BEAKER B, mass of bag decreases indicates diffusion through the bag into starch solution Iodine solution diffuses through the bag into the beaker, causing the Blue-Black change No starch has diffused into the bag, hence no change of Brown colour The tubing acts as a semi permeable membrane, allowing water and iodine solutions to pass through pores, but not allow starch - Perform a first-hand investigation to demonstrate the difference between osmosis and diffusion BEAKERS C AND D The mvt of water from areas of low(glucose) concentration to high(glucose) concentration Beaker Mass of tubing bag (g) Colour after a day in beaker Colour after a day in bag A Initial: 24.6 Final: 30.2 Brown Blue-black B Initial: 22.4 Final: 18.6 Blue-Black Brown

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  • - Perform a first-hand investigation to model the selectively permeable nature of a cell membrane

    Beakers A and B

    The dialysis tubing has small pores which allow certain substances of appropriate size to pass through.

    Allows iodine and water to pass through but stops large starch molecules

    Analysis Increase of mass of bag in BEAKER A indicates osmosis occurring, through

    tubing into the bag (WATER) Colour of Iodine: BROWN > BLUE-BLACK by presence of starch BLUE-BLACK colour of bag indicates that iodine has diffused through the tubing

    and since the Iodine solution in the beaker did not change, no starch diffused out In BEAKER B, mass of bag decreases indicates diffusion through the bag into

    starch solution Iodine solution diffuses through the bag into the beaker, causing the Blue-Black

    change No starch has diffused into the bag, hence no change of Brown colour The tubing acts as a semi permeable membrane, allowing water and iodine

    solutions to pass through pores, but not allow starch

    - Perform a first-hand investigation to demonstrate the difference between osmosis and diffusion

    BEAKERS C AND D

    The mvt of water from areas of low(glucose) concentration to high(glucose) concentration

    Beaker Mass of tubing bag (g) Colour after a day in beaker

    Colour after a day in bag

    A Initial: 24.6Final: 30.2

    Brown Blue-black

    B Initial: 22.4Final: 18.6

    Blue-Black Brown

  • The mvt of glucose is in the opposite direction, from high concentration to low concentration

    Analysis In BEAKER C it is clear that the water has diffused into the bag through the

    tubing, from low glucose to high glucose concentration In BEAKER D it is clear that the water has diffused out the bag through the tubing

    into the glucose solution which is much higher concentration Testing with Benedicts Solution it is clear that the GLUCOSE has moved from

    areas of to low concentration(water) Water and Glucose move in opposite directions Water diffuses from a low to high concentration Starch turns from white to purple Glucose from aqua to orange

    Beaker Mass of dialysis tubing bag (G)

    Colour of solution in beaker after a day

    Colour of solution after a day in bag

    C Initial: 23.5Final: 28.2

    Blue to Orange Blue to Orange

    D Initial: 19.6Final: 14.7

    Blue to Orange Blue to Orange