Hepatoprotective Activity of Aqueous Extract of Channa striatus (Haruan) on Co-Amoxiclav-Induced Liver Damage in Adult Male Sprague-Dawley Rats

Hepatoprotective Activity of Aqueous Extract of Channa striatus (Haruan) on Co-Amoxiclav-Induced Liver Damage in Adult Male Sprague-Dawley RatsKaila Donica T. Abeleda, Angela Beatrice L. Arevalo, Kristel Dana A. Badua, Elaine Mae F. Bautista, Bernadette C. Boholano, Marilet T. Borlagdan, Kristine Xandra D. Bunuan University of Santo TomasFaculty of Pharmacy

IntroductionObjectivesResearch ImpedimentsChanna striatus commonly known as Haruan in Malaysia or Dalag in the Philippines an obligate air-breathing freshwater fish which inhabits all types of water bodies across tropical and subtropical Asian countries dark brown in colour with faint black bands adult Dalag typically measures 15-30 cm

Taxonomic Classification of Channa striatus Kingdom: Animalia Subkingdom: Bilateria Phylum: Chordata Class: Actinopterygii Order: Perciformes Family: Channidae Genus: Channascopoli Species: striata Scientific name: Channa striatus Constituents responsible for the Hepatoprotective Activity of Channa striatusGlycine has the highest concentration among the amino acids present in the Haruan extract Arginine also neutralizes ammonia, which helps in liver detoxification and regenerationGlutamine in decreasing the serum levels of aspartate aminotransferase and total bilirubin MethodologyResearch DesignExperimental study will be used as the research design in order to determine the necessary data.Standard Reagents and ChemicalsThe reagents and chemicals to be used are distilled water, 20% physiological saline, potassium phosphate buffer, and formaldehyde.

Dalag will be gathered from Tarlac City, Tarlac. The fish gathered will be processed for identification at the Bureau of Fisheries and Aquatic Resources afterwards.

Preparation of Aqueous Extract from Channa striatus (Dalag)

Acute Toxicity Study Using Limit TestTo determine doses of Channa striata extract to be administered.Based on OECD 425 guidelines10Acute Toxicity Test for Aqueous Extract of Channastriatus using Limit Test

Hepatoprotective Activity Test using Sprague-Dawley Rats

Determination of ALT, AST and ALP concentrations

Determination of GSH, Lipid Peroxide, and Catalase Levels

Histopathological Examination of Livers

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