PACEapplication Maitra

8/14/2019 PACEapplication Maitra

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Dr. Radhashree Maitra,1580 Pelham Parkway SApt # 1HBronx, NY-10461

Biology and Health Sciences Search Committee

Dean, Dyson College of Arts and Sciences

Pace University

One Pace Plaza

New York, NY .

15th December 2009

Ref: Application for the position of Assistant Professor Molecular and Cellular Biology.

Dear Sir,

Please find complied in .pdf format my complete application for your kind

consideration for the position of Assistant Professor in Molecular and Cellular Biology. My

Ph.D. degree is in Biophysics, Molecular Biology and Genetics and I have several years of

research experience Molecular Biology and Cell Biology with publications as indicated in my

attached Curriculum Vitae.

At Present I am an Assistant Professor at Albert Einstein College of Medicine

of Yeshiva University where I perform only hardcore scientific research and I am in search of a

position, which would give me the opportunity to teach undergraduate and graduate students

along with ample scope of scientific research. The multidisciplinary experience that I have

gathered over the years gives me confidence that if given a chance I would become a valuable

member of the department of Biology.Although I had no formal teaching experience except for my curriculum bound

Teaching assistantship during my graduate studies I have started teaching as adjunct Assistant

Professor at Lehman College CUNY to gather experience and develop expertise.

If my application gathers a favorable consideration I shall be happy to deliver a

talk and discuss my research potentials.

Kindly feel free to contact me for any additional information.

Thank you for your kind consideration.

Sincerely Yours

Radhashree Maitra

.


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RADHASHREE MAITRA

1580 Pelham Parkway South, # 1H, Bronx, New York 10461.: (718) 801-1359e-mail: [email protected]

Career Objective

To secure a position with potential of advancement where I can effectively utilize my expertise as

a scientist and educator to work through positive interaction with students to attain globalstandards in performance.

Summary of Education

Yea

r

Institution level Subject Grade

1996 Calcutta University Ph.D Biophysics, Molecular Biology & Genetics

1989 Calcutta University MS Biochemistry I

1987 Calcutta University BS Chemistry honors Physics & Mathematics I

Professional Experience

Assistant Professor , Department of Surgery and

Department of Pathology, Albert Einstein College of Medicine,

Bronx, NY

2006-2008 NIH training Research Fellow Department of Pathology,

Albert Einstein College of Medicine, Bronx, NY2002- 2006 Research Associate, Department of Pathology, Albert Einstein College

of Medicine, Bronx NY

2001-2002 Research Associate in Department of Biotechnology, Jadavpur

University, Calcutta, India

1999-2001 Research Scientist in Bioinformatics Calcutta University, Calcutta,India.

1996.1999 Research Associate Department of Biology Washington University,St.Louis MO.

Teaching Experience

1990-1996 Graduate Student Department of Biophysics and MolecularBiology, University of Calcutta, India.

Teaching three courses of Biochemistry and three courses of Molecular

Biology per semester to undergraduate classes as Teaching Assistant as a stipulated part of the

Ph.D. curriculum of Calcutta University.

Research support NIH training grant NS 07098
mailto:[email protected]:[email protected]:[email protected]


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Publications

1.Maitra R, Clement CC, Scharf B, Crisi GM, Chitta S, Paget D, Purdue PE, Cobelli N,

Santambrogio L.Endosomal damage and TLR2 mediated inflammasome activation by alkane

particles in the generation of aseptic osteolysis. Mol Immunol. 2009 Oct 3. [Epub ahead of

print]

2. Maitra R, Sadofsky M.J. "A WW-like module in the RAG1 N-terminal domain contributes to

previously unidentified protein-protein interactions. "Nucleic Acids Res.2009 Jun; 37 (10):3301-

9.

3. Maitra, R., Clement, C.C., Crisi, G.M., Cobelli, N., Santambrogio L.Immunogenecity of

modified alkane polymers is mediated through TLR1/2 activation. PLoS ONE. 2008 Jun 18;3

(6):e2438

4.. Bunbury, A., Potolicchio, I., Maitra, R., Santambrogio L. Functional analysis of monocyte

MHC class II compartments. FASEB J. 2009 Jan;23(1):164-71

5..Zak, E.A.., Norling, B., Maitra, R., Huang F., Anderson, B., and Pakrasi, H.B. (2001) The

initial steps of biogenesis of cyanobacterial photosystems occur in plasma membranes.Proc NatlAcad Sci U S A. 98, 13443-8.

6.Inagaki, N.,Maitra, R.,Satoh, K. and Pakrasi, H.B. (2001) Amino acid residues that are

critical forin vivo catalytic activity of CtpA, the carboxyl-terminal processing protease forthe D1 protein of photosystem II. J Biol Chem, 276, 30099-105.

7.Maitra, R. and Thakur, A.R. (1993) Multiple fragment ligation on glass surface: A

novel approach. Indian Journal of Biochemistry and Biophysics, 31, 97-99.

8. Maitra, R. and Thakur, A.R.(1993) Hydroxyl radical induced DNA damage: choice of

an in vitro model.International Journal of Toxicology, Occupational and EnvironmentalHealth. 56, 6-7.

9. Maitra, R. and Thakur, A.R. (1992) Silanisation of glass bound baked DNA permits

enhanced polymerization by DNA polymerase. Current Science62, 586-588.
http://www.ncbi.nlm.nih.gov/pubmed/19804908?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSumhttp://www.ncbi.nlm.nih.gov/pubmed/19804908?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSumhttp://www.ncbi.nlm.nih.gov/pubmed/18560588?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSumhttp://www.ncbi.nlm.nih.gov/pubmed/18560588?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSumhttp://www.ncbi.nlm.nih.gov/pubmed/18815360?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSumhttp://www.ncbi.nlm.nih.gov/pubmed/19804908?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSumhttp://www.ncbi.nlm.nih.gov/pubmed/19804908?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSumhttp://www.ncbi.nlm.nih.gov/pubmed/18560588?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSumhttp://www.ncbi.nlm.nih.gov/pubmed/18815360?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum


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Scientific meetings

Endosomal damage and TLR2 mediated inflammasome activation by alkane particles in

the generation of aseptic osteolysisRadhashree Maitra, Cristina C. Clement, Brian Scharf,Giovanna M, Crisi, Sriram Chitta Daniel Paget, P. Edward Purdue, Neil Cobelli, Laura

Santambrogio. Albert Einstein College of Medicine (AECOM), Bronx, NY, USA. Oral

Presentation. 4th International Meeting on UHMWPE for arthroplasty from powder to debris,16th -18th September 2009 . Turin, Italy.

Self-Peptidomic Repertoire of the human pre-nodal lymph.

Clement, Cristina C.; Maitra, Radhashree, Sahu, Ranjit, Santambrogio, Laura. Pathology,

Albert Einstein College of Medicine (AECOM), Bronx, NY, USA. Young Investigator Award

talk, 21st American Peptide Symposium, Bloomington, Indiana-July 2009.

Binding of modified alkane polymers to human recombinant TLR-2 receptor monitored

by intrinsic Tyr fluorescence. Clement, Cristina C.; Maitra, Radhashree Santambrogio,Laura. Pathology, Albert Einstein College of Medicine (AECOM), Bronx, NY, USA.

Abstracts of Papers, 236th ACS National Meeting, Philadelphia, PA, United States, August 17-

21, 2008 (2008), BIOL-178.

Binding of Modified Alkane Polymers to Human Recombinant TLR-2 Receptor

Monitored by Intrinsic Tyr Fluorescence. Clement, Cristina C., Maitra, RadhashreePathology, Albert Einstein College of Medicine (AECOM), Bronx, NY, USA. Abstracts,

40th Middle Atlantic Regional Meeting of the American Chemical Society, Queens, NY, United

States, May 17-21 (2008), MRM-259. Publisher: American Chemical Society, Washington,

D. C

Immunogenecity of modified alkane polymers is mediated through TLR1/2 activation.

Clement, Cristina C.; Maitra, Radhashree; Crisi, Giovanna M.; Cobelli, Neil; Santambrogio,Laura. Pathology, Albert Einstein College of Medicine (AECOM), Bronx, NY, USA.

Abstracts. 48th American Cell Biology (ACB) National Meeting, San Francisco, December 13-17, 2008.

Carboxyl terminal protease ofsynechocystis 6803 and their role in photosynthesis.

Maitra, R., Ivleva, N.B., Inagaki, N., Satoh, K., Shestakov, S.V. and Pakrasi H.B.The plant retreat meeting of the Department of Biology Washington University, St.Louis,

Missouri on 2nd May 1998 at trout lodge, Missouri, USA.

Ctpa a c-terminal processing protease inviolved in the biogenesis of photosystem II

complex.

Maitra, R., Inagaki, N.,Anbudurai, P.R. and Pakrasi H.B.The plant retreat meeting of the Department of Biology Washington University,

St.Louis,Missouri on 17th April 1997 at Meramec, Missouri, USA.

Biochemical activities of glass bound DNA.

Maitra, R. and Thakur, A.R.

Diamond Jubilee Meeting of the SOCIETY OF BIOLOGICAL CHEMISTS (SBC) INDIA on28th December 1991 at Calcutta.


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Honors

Winner of "SHANTI BHAKTA MEMORIAL AWARD" for the best speaker at the student

seminar held between 15th-21st January 1990 in the Department of Biochemistry ,Calcutta

University.

Qualified at the "ALL INDIA NATIONAL ELIGIBILITY TEST (NET)"of 1989 in LIFE

SCIENCES and was awarded scholarship and eligibility to carry out research for theattainment of doctorate degree from any Indian University or Institute. The scholarship was

valid for five years.

Qualified for Senior Research Fellowship at all India level in the examinations conducted by

"COUNCIL FOR SCIENTIFIC AND INDUSTRIAL RESEARCH (CSIR) of 1993. The

fellowship was valid for three years.

Membership

.Member of the "SOCIETY OF BIOLOGICAL CHEMISTS (INDIA)".

Member of "INDIAN BIOPHYSICAL SOCIETY".

. Member of NEW YORK ACADEMY OF SCIENCE

Scientific Review

Invited reviewer of Journal of Clinical and developmental Immunology.

Technical skills

Cell biology and Immunology: Tissue culture, tissue specific typing, isotyping, purification ofmonocytes, dendritic cells and macrophages from blood, bone marrow, as well as spleen, thymus

and lymph node. Cell marker specific staining, FACS analysis, ELISA, sandwich ELISA,

immuno-histochemical staining, confocal microscopy, animal immunization, cell proliferation

assay, peptide elution. Molecular biology: PCR. RT-PCR, DNA cloning vector construction, site directed

mutagenesis, southern blotting, c-DNA library construction and screening, northern blotting, S1

analysis, DNA sequencing, EMSA, Chip Assay, primer extension, tissue culture, transfection,

western blotting. Immunoblotting. Yeast two hybrid assay.

Biophysics: Spectroscopy, flourometry, circular dichroism, mass spectroscopy, electron

microscopy and HPLC.

Protein chemistry: Over-expression of proteins, protein purification by various methods

including FPLC, electrophoresis including SDS-PAGE, native gradient and two dimensional gels,

immunoblotting and ELISA. In vitro biochemical analysis of the proteases by

extensive use of HPLC using different synthetic peptides and organic compounds.

Computer skill: Have used Machintosh and IBM based programs such as microsoft office,

Endnote Plus, Canvas, Adobe photoshop, photo deluxe, Flash, DNA star, sigma plot, GCG blast

and Entrez software. Also have knowledge of Web page designing by HTML, DHTML, Visual

basic, Visual C++, Front Page, Java and Java script.

Legal Status: Permanent Resident.


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LIST OF REFERENCES:

1. Dr. Rachel Hazan,

Associate Professor

Albert Einstein College of Medicine1300 Morris Park Avenue

Forchheimer Building, Room 529SBronx, NY 10461

Tel: (718) 430-3349

Fax: (718) 430-8541

Email: [email protected]

2. Dr. M.J. Sadofsky,

Associate Professor

Department of Pathology,

Albert Einstein College of Medicine,

1300, Morris Park Avenue

Bronx, NY 10461

Tel: (718) 430-2222Email: [email protected]

3. Prof. Ashoke Ranjan Thakur

Vice ChancellorWest Bengal State University Barasat

Berunantukuria, Mallikapur, pin-700126

Tel: 011913325241977 (Off)

011919831179909 (Cell)

011913325241977 (Fax)

Email: [email protected] and [email protected]
mailto:[email protected]:[email protected]:[email protected]:[email protected]:[email protected]:[email protected]:[email protected]:[email protected]:[email protected]


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Scientific Interest/ Research Plan

My scientific interest is focused on different aspects of molecular recognition processes

especially those that can explain, at the molecular level the pathological conditions in certain

autoimmune diseases like rheumatoid arthritis and artificial implant related osteolysis.

My primary investigations at Albert Einstein College of Medicine-Pathology Department,

conducted in the laboratory of Professor Laura Santambrogio, are focused primarily on the role of

immature dendritic cells (DCs) in the development of osteolytic necrosis post artificial prosthesis

implantation. We used the mouse model of Ultra High Molecular weight polyethylene

(UHMWPE) induced calvarial osteolysis to follow the precise role played by DCs. We

investigated even further the critical contribution of the circulating DCs in csf-1r -/- knockout

mice with severely compromised macrophages and osteoclasts. The findings are already under

journal review. The project was initiated with in vitro analysis of the effect of modified and

unmodified as well as patient retrieved UHMWPE in DC activation. The effects were confirms

by electron microscopy ,immuno-histochemical staining, surface marker staining followed by

flow cytometry, genechip analysis, luciferase assay as well as by FTIR and mass spectroscopy.

The interesting observation that surface oxidized UHMWPE is by far a stronger immunogen and

that Toll like receptor 1/2 play a critical role in the recognition of the non biologic organicpolymer were published in 2008 and was the basis of the previously mentioned in vivo studies.

I was also actively involved in the peptidomics and proteomics analysis, utilizing high resolution

mass spectroscopy ESI-LTQ and MALDI-Tof-Tof MS/MS to sequence peptides isolated from the

human lymph and determine, for the first time in the field, the self-peptidomic carrying ability of

the lymph. This investigation allowed the discovery of a new set of peptides that may have

immuno-dominant potential, and therefore might be involved in the maintenance of peripheral

self tolerance or, might stimulate autoimmune diseases, such as rheumatoid arthritis.

Previous to my work on osteo-immunology, I worker with Prof. Sadofsky at the same institution

where I was involved in elucidating the precise role of recombinase proteins RAG1 and RAG2 in

V(D)J recombination. During the studies I have identified and characterized a WW like domain

in the N-terminal region (NTD) of RAG1 protein that has high propensity to bind to proline rich

region. This might explain the biological role of the evolutionarily conserved NTD even when it

does not directly contribute to the recombination reaction. During Yeast-2-Hybrid analysis using

NTD as bait few preys were identified and transcription factor GMEB-1 was one of them. I have

cloned purified characterized GMEB-1 and compared its characteristics with well characterized

transcription factor Pax5. The RAG-1 NTD binds strongly to Pax5 and the interactions have

been well characterized.

During my research at Washington University I isolated cloned and characterized CtpA a C-terminal specific protease found in Synecocystis 6803. Site directed mutagenesis was utilized to

identify the amino acids critical for the protease activity. Several temperature sensitive mutants

were generated in the process. Furthermore the membrane biogenesis of Photosystem 1 was also

elucidated.

My graduate studies were done on different structural forms of DNA. The behavior of each

structural form of DNA to processing enzymes like topoisomerase, Ligase , polymerases andrecombinases were extensively studied. SSB or single stranded DNA binding protein was utilized

to evaluate the extent of single strand region present in DNA which was further confirmed by

fluorescence and fluorescence acrylamide quenching studies.

The scientific experience and technical expertise that I have gathered over the years gives me the

confidence to explore and answer any biological question. All the same I would prefer to do

translational research where I would find the cause and cure of specific pathological conditions

using different biophysical and biotechnological techniques. Given a chance I would like to

continue my investigations in the field of osteo-immunology.


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TEACHING PHILOSOPHY

My teaching philosophy is based on a fundamentally optimistic outlook. I believe thateducation is a solution to many problems. To educate is to become better educated. It is a

chained process that improves with time. As a teacher I would encourage excellence in

my students and myself. I would urge myself to develop course material that ischallenging and interactive. I strongly believe that using a variety of multimedia

including videos, slides and the Internet is an excellent way to compliment traditional

course lectures and laboratory exercise. Nonetheless reading coupled with traditionallectures remains the foundation of the educational experience.

Students learn best when they construct their own knowledge through exploration and

discussion. I wish to use student exploration as an instructional method as it will allow

students to be active contributors in the classroom and take responsibility for their ownlearning. Classroom discussion is an essential part of student education as this is the

space where students use language to synthesize their knowledge and make their work

public. I feel I can encourage students to seek new knowledge and explore their own

ideas by an enthusiastic presentation of both what is, and what is not known about thesubject.

I believe that all of my students, with the help of the right tools, will be able to overcomeany difficulties that the understanding and analysis of science might present. I do not

mean that every student who enters my class will be able to understand by him or herself

equally simply as a result of how I present the lesson, but rather that each of my student

will be guaranteed the opportunity to learn and analyze by him or herself to the best ofhis or her abilities. I believe that one of my best qualities, as a teacher is my ability to

inspire confidence in my students so that they feel comfortable expressing themselves

regardless of their level of abilityIn my interaction with students, I will always be respectful, especially when dealing with

differing opinion that may arise as a result of scientific thinking. I have to constantly

realize that as a teacher I am a facilitator of the process. I want my students to learn tothink critically and not merely accept my ideas and opinion. My drive to pursue higher

education and become a scientist was due in part to several teachers who inspired me and

helped me learn the skills necessary to accomplish these goals. I would like to be thecatalyst for other people to pursue their dreams.

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PACEapplication Maitra