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Principles of Biology, Lab 2 and summative assessment.

Bachelor of Applied Science

NSCI 5104, Lab 2, Lab assessments 2011. Unitec Institute of Technology, March 2011. 2

Principles of Biology

Course NSCI 5104

FormativeLaboratory

Assignment 1

TranscriptionTranslation

Submission Date:Tuesday April 5th, 2011

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Assessment Brief

Tutor: Steve ChambersCovers: Learning outcomes 2-4Value: If this exercise is chosen for grading it will count for 10% of the final course gradeDate due: Tuesday April 5th, 2011

The aim of this assignment is for students to:

Become familiar with base pairing that compose DNA and RNA

Understand transcription & translation

Understand the triplet code

Observe the effects of changes in the DNA sequence

Submission Requirements:

Submit your assignment in electronic copy to the assignment drop-box in Moodle, or hardcopy to the assignment drop-box on level 3, building 115.

The work submitted must be your own work with no plagiarism.

Marking schedule is available for each course assessment to assist you with assignmentcontent.

Resources Available.

Information should be gathered from a number of sources: Books, papers and journals

Course material

Web

Lectures

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Assignment 1 Transcription Translation

You are provided with several pages of sequence data from the Human Genome.Select a pair of sequences (e.g. 1a and 1b) from this page then:

1. Write out the other sequences (anti-sense) needed to complete the double strandedDNA molecule, for both sequences in the pair.

2. When transcription starts the strands separate to allow RNA to make a copy. Write outthe corresponding RNA base for each DNA base (sense strand) to make RNA strands forboth sequences.

3. Examine the RNA strands and look for the correct starting position for the translationprocess. This will correspond to the Met amino acid (use the universal genetic code chart

provided). Highlight an appropriate Start triplet on your sequence. Make sure you alsoidentify and highlight a suitable end code (Ter/terminator).

4. Write out the amino acid sequences that forms polypeptides, between the start andstop triplets

5. Show how your amino acid sequence corresponds to your RNA sequence.

6. Continue the process until you have accounted for you entire original paragraphs ofDNA sequences

7. Compare the 2 sequences in your pair and try to work out what is the change betweenthe 2 sequences, and what effect this had on the peptides.

NB- these sequences are available on Moodle if you wish to directly work on a computer.

For those interested the following is the summary information from which thesesequences were derived from GenBank (prior to being altered for this lab).

1: >gi|284181769|gb|GU454848.1| Influenza A virus (A/swine/Thailand/CU-

CB1/2006(H1N1)) segment 4 hemagglutinin (HA) gene, complete cds

2: >gi|310884785|gb|CY076717.1| Influenza A virus

(A/ferret/Washington/WADDL13230/2009(H1N1)) segment 4 sequence

3: >gi|308742922|gb|CY075840.2| Influenza A virus (A/Thailand/CU-

H88/2009(H1N1)) segment 6 sequence

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Sequence 1a

TGAGTAAAATGAAGGCAATACTATTAGTCTTGCTATGCACACTTGCAGCAGCAAAT

GCAGACACACTGTGTATAGGTTATCATGCAAATAATTCAACTGACACTGTTGATAC

AGTATTAGAAAAGAATGTTACAGTAACACACTCTGTTAACCTTTTAGAAGACAGAC

ACAATGGAAAACTATGTAACCTAAGGGGGAAGGCCCCACTGCATTTGGGTAAATGT

AACATTGCCGGATGGCTCCTAGGAAACCCAGAGTGCGAATTACTATT

Sequence 1b

TGAGTAAAATGAAGGCAATACTATTAGTCTTGCTATGCACACTTGCAGCAGCAAAT

GCAGACACACTGTGTATAGGTTATCATGCAAATAATTCAACTGACACTGTTGATAC

AGTATTAGAAAAGAATGTTACAGTAACACACTAGTTAACCTTTTAGAAGACAGACA

CAATGGAAAACTATGTAACCTAAGGGGGAAGGCCCCACTGCATTTGGGTAAATGTA

ACATTGCCGGATGGCTCCTAGGAAACCCAGAGTGCGAATTACTATT

Sequence 2a

TTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCGAACAATTCAACA

GACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCT

TCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGAGGGGTAGCCCCATTGC

ATTTGGGTAAATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCA

CTCTCCACAGCAAGCTCATGGTCCTACATTTGGAAACATCTAGTTCA

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Sequence 2b

TTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCGAACAATTCAACA

GACACTGTAGACACAGTACTAGAAAAGAATGTAACTCACACAATGACTGTTAACCT

TCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGAGGGGTAGCCCCATTGC

ATTTGGGTAAATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCA

CTCTCCACAGCAAGCTCATGGTCCTACATTTGGAAACATCTAGTTCA

Sequence 3a

AACACACTCAAATGAATCCAAACCAAAAGATAATAACCATTGGTTCGGTCTGTATG

ACAATTGGAATGGCTAACTTAATATTACAAATTGGAAACATAATCTCAATATGGAT

TAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGCAATCAAAGCG

TCATTACTTATGAAAACAACACTTGGGTAAATCAGACATATGTTAACATCAGCAAC

ACCAACTTTGCTGCTGGACAGTCAGTGGTTTCCGTGAAATTAGCGGGCA

Sequence 3a

AACACACTCAAATGAATCCAAACCAAAAGATAATAACCATTGGTTCGGTCTGTATG

ACAATTGGAATGGCTAACTAATATTACAAATTGGAAACATAATCTCAATATGGATT

AGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGCAATCAAAGCGT

CATTACTTATGAAAACAACACTTGGGTAAATCAGACATATGTTAACATCAGCAACA

CCAACTTTGCTGCTGGACAGTCAGTGGTTTCCGTGAAATTAGCGGGCA

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UNIVERSAL GENETIC CODETRANSLATION TO AMINO ACID SEQUENCE

U C A G

U UUUUUC

Phe UCUUCCUCAUCG

Ser UAUUAC

Tyr UGUUGC

Cys UCAG

UUAUUG

Leu UAAUAG

Ter/stop UGA Ter/stop

UGG Trp

C CUUCUCCUACUG

Leu CCUCCCCCACCG

Pro CAUCAC

His CGUCGCCGACGG

Arg UCAGCAA

CAGGln

A AUUAUCAUA

Ile ACUACCACAACG

Thr AAUAAC

Asn AGUAGC

Ser UCAG

AAA

AAG

Lys AGA

AGG

Arg

AUG MetG GUU

GUCGUAGUG

Val GCUGCCGCAGCG

Ala GAUGAC

Asp GGUGGCGGAGGG

Gly UCAGGAA

GAGGlu

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Marking Schedule for Assignment (Lab 2)

Area Mark Comment

Sense to Anti-sense

Correct alignment and assignment of bases 5 marks

Transcription

Correct alignment and assignment of bases 5 marks

Translation

Identification of an appropriate start and stop triplet s 5 marks

Translation of amino acid sequence 5 marks

Identification of amino acid sequence against the originalDNA/RNA sequence 5 marks

Identification of the change between sequences 5 marks

Total 30 marks (100%)

GRADE

Assessor: .. Signature:

Date:

NSCI 5104, Lab 2, Lab assessments 2011. Unitec Institute of Technology, March 2011. 9

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