Ning ILA1 Figs suppl - Plant Cell · Complementation Test of ila1. ( A ) Constructs for complementation and biochemical assays. ( B ) Leaf angle comparison among the complementation

Supplemental Figure 1. ila1 Shows Increased Leaf Angle.(A) Co-segregation analysis of the phenotype and genotype in the T-DNA insertion mutants. 1-20 are twenty F2 plants generated by crossing an ila1 with a wild-type plant. h, hybrid; w, wild type; m, mutant. The plants having the h and w genotypes show the wild-type appearance and the ones having m genotype show the increased leaf angle. (B) Kinetic analysis of leaf angle between the first and the second leaves of ila1 and wild-type plants (mean of 12 plants ± SE).(C) Leaf angle between the first and the second leaves during leaf development. The developmental stages are defined by the ratio of the first leaf length to that of the second leaves (mean of 12 plants ± SE).

APrimer

h w m m w w w m m m m m mww w w wh h

a+bb+c

1819 201 2 3 4 5 6 7 8 9 10 11 12 13 14 1516 17

B C

0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.60

20

40

60

80

100

Ratio of the leaf length (1st /2nd)

WTila1

2 4 6 8 100

20

40

60

80

100WTila1

Days

Leaf

ang

le (°

)

Leaf

ang

le (°

)

Supplemental Data. Ning et al. Plant Cell. (2011). 10.1105/tpc.111.093419

1

Supplemental Figure 2. ila1 Shows Indistinguishable BR Responses compared to

the Wild Type.(A) and (B) The seedlings of ila1 and wild type were germinated and grown on 1/2 MS medium containing different concentration of BR. The lengths of primary root (A) and coleoptile (B) were examined after seven days of germination. Values are the means ±SE (n = 30).(C) to (H) Scanning electron microscopic examination of leaf lamina joint. (C) and (D) Adaxial surface of the wild-type (C) and ila1 (D) leaf lamina joint. (E) and (F) Longitudinally sectioning of the wild-type (E) and ila1 (F) leaf lamina. The outlines of longitudinal sections are shown by broken lines, indicating the increased leaf angle in ila1(arrow heads). (G) and (H) The regions shown are denoted by rectangles in (E) and (F), respectively. Bars = 0.5 mm in (C) to (F), 0.1 mm in (G) and (H). Ad, adaxial; Ab, abaxial.

BA

2

4

6

8

0 0.5 1 2BR (µM)

WTila1

Roo

t len

gth

(cm

)

0 0

2

4

6

8

0 0.5 1 2BR (µM)

Col

eopt

ilele

ngth

(mm

)

WTila1

Ad Ad

Ad Ad

AdAd

AbAb

C D E F

G H


2

Supplemental Figure 3. Complementation Test of ila1.(A) Constructs for complementation and biochemical assays.(B) Leaf angle comparison among the complementation lines (pILA1-1 and pILA1-2), ila1, and wild-type plants at the tillering stage (mean of 15 plants ±SE). **P <0.01 (t-test).(C) Phenotypic observation among the complementation lines (pILA1-1 and pILA1-2), ila1, and wild-type plants at the tillering stage.

ila1 WT pILA1-1 pILA1-2

C

B

ila1WT pILA1-1 pILA1-20

10

20

30

40

50

60

**

Leaf

ang

le (°

)

A

ATG TAA

21350 8059pILA1

8800

ATG 3xFLAG

0 1692pILA1cF


3

Supplemental Figure 4. Sequence Alignment of ILA1 with Identified Plant MAPKKKs.Shaded boxes indicate identical residues. The eleven subdomains of protein kinase are indicated with roman numerals. A conserved lysine in the ATP binding site is indicated by an arrow. Prefixes on protein names indicate species of origin. At, Arabidopsis thaliana; Le, Solanum lycopersicum var. lycopersicum; Os, Oryza sativa.


4

Supplemental Figure 5. Identification of IIP-ILA1

Interactions by Yeast Two-Hybrid Assay.The interaction were verified by growing yeast on a selective medium (SC/-Leu-Trp-His with 3-AT) and by conducting β-Gal assays.

IIP1-GAL4-AD

IIP2-GAL4-AD

IIP3-GAL4-AD

IIP4-GAL4-AD

IIP5-GAL4-AD

IIP6-GAL4-AD

GAL4-AD

ILA1-GAL4-DB GAL4-DB

20 mM 3-ATSC/-Leu-Trp SC/-Leu-Trp-His β-Gal assay


5

Supplemental Figure 6. Sequence Alignment and Phylogenetic Tree of the IIP Members.(A) Multiple sequence alignment of IIPs in rice (IIP1-6), the homologs from Arabidopsis (At1g64140, At5g09670, and At5g64550), and At WRKY19. Shaded characters indicate identical residues. The black box indicates the conserved domain.(B) Phylogenetic tree of the proteins shown in (A).

A

B

Subfamily B

Subfamily AAt1g64140

IIP2

100 IIP1

86

At5g09670

At5g64550

100

IIP5IIP3

IIP4100

76

100

74At WRKY19

0.1

IIP6


6

A

B

C

Zhenshan 97

Minghui 63

Rel

ativ

e in

tens

ityR

elat

ive

inte

nsity

Rel

ativ

e ex

p. le

vel t

o U

BQ

5

Leaf sheathe

InternodeNode

Lamina jointLeaf

Flower

IIP1IIP2IIP3IIP4IIP5IIP6

0

0.1

0.2

0.3

0.4

0.5IIP1IIP2

IIP3IIP4IIP5IIP6


7

Supplemental Figure 7. Expression Profiles of IIPs.(A) qRT-PCR analysis of IIPs expression in different rice organs. The error bars represent the standard errors (SEs) of the mean values of two biological replicates.(B) and (C) The microarray data from different organs of Zhenshan 97 (A) and Minghui 63(B) at various development stages (1-15), showing the expression patterns of IIPs. No probe is available for IIP3.

1: Imbibitional seeds; 2: Embryo and radicle; 3: Roots of seedling with two tillers; 4: Shoots of seedling with two tillers; 5: Stems before heading; 6: Stems at the heading stage; 7: Sheathes while young panicle appears; 8: Sheathes while young panicle is 4-5 cm long; 9: Leaves while young panicle appears; 10: Leaves while young panicle is 4-5 cm long; 11: Hulls before flowering; 12: Stamens before flowering; 13: Spikelets after pollination; 14: Panicles at the heading stage; 15: Young panicles (4-5 cm long).



9

Supplemental Table 1. Compositional Analysis of Wall Residues from Wild-Type and ila1

Leaf Blades

Samples Rhamnose Fucose Arabinose Xylose Mannose Galactose Glucose Cellulose

WT 3.4+0.2 1.3+0.1 57.8+2.7 199.2+4.6 2.1+0.0 24.1+1.6 45.4+2.1 272.9+20.2

ila1 3.7+0.2* 1.3+0.1 59.0+1.7 195.4+2.1 2.1+0.1 24.4+0.9 40.9+2.6 250.2+21.4

Alcohol insoluble residues (AIR) were prepared from the ila1 and wild-type leaf blades as described in

Methods. The alditol acetate derivatives were analyzed by GC-MS. The results are given as the means (mg/g of

AIR) of three replicates ± SD.

*Significance between the wild type and mutant is determined by the least significant difference t test at P <

0.05.


10

Supplemental Table 2. Genes Upregulated Four-Fold in Microarray Analysis of the ila1

Leaf Lamina Joints Compared to That of the Wild Type

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os01g51610 B3 DNA binding domain containing protein 4.5 0.00 LOC_Os09g04624 GDSL-like lipase/acylhydrolase 4.2 0.01 LOC_Os07g29310 OsSAUR30-Auxin-responsive SAUR gene family member 3.4 0.05 LOC_Os03g22620 Terpene synthase family 3.3 0.03 LOC_Os03g10150 DUF623 domain containing protein 3.2 0.01 LOC_Os10g38050 HOTHEAD precursor 3.2 0.03 LOC_Os02g44108 Expansin precursor 3.2 0.03 LOC_Os12g06440 Expressed protein 3 0.02 LOC_Os01g69960 Expressed protein 2.9 0.02 LOC_Os08g02110 Peroxidase precursor 2.8 0.02 LOC_Os01g36294 Cytochrome P450 2.6 0.00 LOC_Os07g03900 Lectin-like receptor kinase 2.6 0.01 LOC_Os04g39150 Pathogenesis-related Bet v I family protein 2.5 0.00 LOC_Os02g44080 Aquaporin protein 2.5 0.03 LOC_Os08g06280 LSD1 zinc finger domain containing protein 2.4 0.02 LOC_Os06g40570 GRAM and C2 domains containing protein 2.4 0.02 LOC_Os02g11640 UDP-glucoronosyl/UDP-glucosyl transferase 2.4 0.03 LOC_Os09g15350 Expressed protein 2.3 0.00 LOC_Os03g40540 Cytochrome P450 2.3 0.03 LOC_Os12g43750 Expressed protein 2.3 0.04 LOC_Os03g52690 CBS domain containing membrane protein 2.3 0.05 LOC_Os06g10510 Oxidoreductase/ transition metal ion binding protein 2.3 0.05 LOC_Os07g04350 Expressed protein 2.2 0.00 LOC_Os01g59550 Senescence-induced receptor-like protein kinase 2.2 0.01 LOC_Os06g49040 Myb-like DNA-binding domain containing protein 2.2 0.03 LOC_Os10g40934 Flavonol synthase/flavanone 3-hydroxylase 2.2 0.03 LOC_Os04g45290 Glycosyl hydrolases 2.1 0.00 LOC_Os07g04350 Expressed protein 2.1 0.00 LOC_Os12g03240 MATE efflux family protein 2.1 0.02 LOC_Os02g55400 ATPase 8 2.1 0.05 LOC_Os06g44320 Expressed protein 2.1 0.05 LOC_Os02g38410 Ubiquitin family protein 2 0.00 LOC_Os11g02840 Protein kinase 2 0.00 LOC_Os02g46660 SNARE associated Golgi protein 2 0.00 LOC_Os10g36580 UP-9A 2 0.01 LOC_Os11g47500 Glycosyl hydrolase 2 0.01 LOC_Os01g10450 Protein kinase 2 0.05 LOC_Os05g50640 Transposon protein 2 0.05

Fold change of the ila1 mutant compared with the wild type. Values are showed as log2 of fold changes.


11

Supplemental Table 3. Genes Downregulated Four-Fold in Microarray Analysis of the ila1

Leaf Lamina Joints Compared to That of the Wild Type

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os01g73770 Dehydration-responsive element-binding protein -7.2 0.02 LOC_Os12g05440 Cytochrome P450 -6.2 0.00 LOC_Os09g31040 EF hand family protein -5.9 0.05 LOC_Os12g39310 Cytochrome P450 -5.8 0.04 LOC_Os09g34230 UDP-glucoronosyl/UDP-glucosyl transferase -5.6 0.00 LOC_Os05g07010 Myb-like DNA-binding domain containing protein -5.4 0.00 LOC_Os11g05380 Cytochrome P450 -5.2 0.00 LOC_Os05g31970 Expressed protein -5.1 0.00 LOC_Os01g65830 Acyl-desaturase -5 0.04 LOC_Os08g28710 Receptor protein kinase -4.8 0.01 LOC_Os04g45730 Protein kinase -4.8 0.01 LOC_Os02g47230 Disease resistance/zinc finger protein -4.8 0.02 LOC_Os07g37730 NADH-ubiquinone oxidoreductase -4.6 0.01 LOC_Os02g26810 Cytochrome P450 -4.5 0.00 LOC_Os03g08520 DUF581 domain containing protein -4.5 0.00 LOC_Os08g06210 Expressed protein -4.5 0.02 LOC_Os08g06210 Expressed protein -4.5 0.02 LOC_Os03g60880 Nucleobase-ascorbate transporter -4.4 0.02 LOC_Os08g36920 AP2 domain containing protein -4.3 0.00 LOC_Os01g50400 STE_MEKK_ste11_MAP3K.5 - STE kinases -4.3 0.00 LOC_Os06g14670 ODORANT1 -4.3 0.01 LOC_Os02g33590 U-box domain-containing protein -4.2 0.00 LOC_Os04g49550 RING-H2 finger protein ATL2A -4.2 0.00 LOC_Os08g06210 Expressed protein -4.2 0.02 LOC_Os08g17160 plastocyanin-like domain containing protein -4.1 0.00 LOC_Os08g17160 Plastocyanin-like domain containing protein -4.1 0.00 LOC_Os05g46750 STE_MEKK_ste11_MAP3K.18 - STE kinases -4.1 0.00 LOC_Os05g29810 AP2 domain containing protein -4.1 0.00 LOC_Os02g54050 Ethylene-responsive transcription factor -4.1 0.00 LOC_Os01g08500 Transposon protein -4.1 0.00 LOC_Os03g46440 Tramtrack, Broad Complex BTB domain -4.1 0.01 LOC_Os08g35600 Tyrosine protein kinase domain containing protein -3.9 0.00 LOC_Os02g14840 Potassium channel KAT1, -3.9 0.00 LOC_Os08g42750 Calmodulin depedent protein kinases -3.9 0.02 LOC_Os12g18560 Invertase/pectin methylesterase inhibitor protein -3.8 0.00 LOC_Os08g39840 lipoxygenase -3.8 0.01 LOC_Os10g28240 Calcium-transporting ATPase -3.8 0.01 LOC_Os07g34260 Chalcone and stilbene synthases -3.8 0.01 LOC_Os01g66860 Serine/threonine protein kinase -3.8 0.01 LOC_Os02g45420 AP2 domain containing protein -3.7 0.00


12

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os04g45590 Glyoxalase family protein -3.7 0.00 LOC_Os12g08130 Amino acid transporter -3.7 0.00 LOC_Os01g60600 WRKY DNA-binding domain containing protein -3.7 0.01 LOC_Os01g70820 Lumenal PsbP -3.7 0.02 LOC_Os04g49370 Expressed protein -3.7 0.02 LOC_Os03g18030 Lucoanthocyanidin dioxygenase -3.7 0.05 LOC_Os08g36920 AP2 domain containing protein -3.6 0.00 LOC_Os03g15270 Gibberellin receptor GID1L2 -3.6 0.00 LOC_Os01g67950 Ubiquitin family protein -3.6 0.01 LOC_Os04g52780 Leucine-rich repeat receptor protein -3.6 0.03 LOC_Os03g10640 calcium-transporting ATPase -3.6 0.03 LOC_Os11g29290 Cytochrome P450 -3.6 0.04 LOC_Os02g48320 DNA binding protein -3.5 0.00 LOC_Os06g11650 Phosphate-induced protein -3.5 0.00 LOC_Os05g41610 Glycosyl hydrolases family 17 -3.5 0.00 LOC_Os01g60600 WRKY DNA-binding domain containing protein -3.5 0.01 LOC_Os02g08270 Class I glutamine amidotransferase -3.5 0.01 LOC_Os02g48770 SAM dependent carboxyl methyltransferase -3.5 0.01 LOC_Os01g50370 STE_MEKK_ste11_MAP3K.4 - STE kinases -3.5 0.01 LOC_Os01g63930 Cytochrome P450 -3.5 0.02 LOC_Os04g57350 EH domain-containing protein 1 -3.5 0.03 LOC_Os03g13740 Immediate-early fungal elicitor protein CMPG1 -3.4 0.00 LOC_Os03g47280 VQ domain containing protein -3.4 0.00 LOC_Os01g50940 Helix-loop-helix DNA-binding domain protein -3.4 0.00 LOC_Os01g13930 Expressed protein -3.4 0.00 LOC_Os03g57640 Gibberellin receptor GID1L2 -3.4 0.00 LOC_Os08g28710 Receptor protein kinase CRINKLY4 -3.4 0.01 LOC_Os08g39290 EF hand family protein -3.4 0.01 LOC_Os05g39250 Phosphatidylethanolamine-binding protein -3.4 0.01 LOC_Os05g46840 Proline-rich protein -3.4 0.01 LOC_Os08g36910 Alpha-amylase precursor -3.4 0.02 LOC_Os05g37660 Transferase family protein -3.4 0.03 LOC_Os03g56260 Kinesin motor domain containing protein -3.4 0.04 LOC_Os07g09670 Galactosyltransferase family protein -3.3 0.00 LOC_Os03g32420 Expressed protein -3.3 0.00 LOC_Os01g72360 Expressed protein -3.3 0.00 LOC_Os07g34640 Pyruvate Pi dikinase regulatory protein -3.3 0.00 LOC_Os02g41670 Phenylalanine ammonia-lyase -3.3 0.00 LOC_Os12g35610 Respiratory burst oxidase -3.3 0.00 LOC_Os09g20000 Heavy metal-associated protein -3.3 0.00 LOC_Os12g09640 Protein phosphatase 2C -3.3 0.00 LOC_Os01g03980 Expressed protein -3.3 0.00 LOC_Os09g17146 Expressed protein -3.3 0.00


13

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os03g57880 Glucan endo-1,3-β-glucosidase -3.3 0.01 LOC_Os01g66860 Serine/threonine protein kinase -3.3 0.01 LOC_Os01g13950 OsGrx_A1-glutaredoxin subgroup III -3.3 0.01 LOC_Os03g58900 Galactosyltransferase family protein -3.3 0.01 LOC_Os07g09190 Transketolase -3.3 0.02 LOC_Os09g21290 Expressed protein -3.3 0.02 LOC_Os02g34410 U-box domain-containing protein -3.3 0.04 LOC_Os04g46980 cis-zeatin O-glucosyltransferase -3.3 0.04 LOC_Os07g36590 Serine/threonine-protein kinase -3.3 0.05 LOC_Os02g56380 OsWAK21-OsWAK receptor-like cytoplasmic kinase -3.2 0.00 LOC_Os09g25060 OsWRKY76 -3.2 0.00 LOC_Os08g34510 Hypothetical protein -3.2 0.00 LOC_Os09g36320 Tyrosine protein kinase -3.2 0.00 LOC_Os01g08500 Transposon protein -3.2 0.00 LOC_Os01g50410 STE_MEKK_ste11_MAP3K.6 - STE kinases -3.2 0.01 LOC_Os10g40640 Glycosyl transferase 8 -3.2 0.01 LOC_Os05g46350 Calmodulin-binding protein -3.2 0.01 LOC_Os02g48190 Expressed protein -3.2 0.01 LOC_Os02g45160 Aluminum-activated malate transporter -3.2 0.02 LOC_Os06g46980 Hypothetical protein -3.2 0.02 LOC_Os01g47580 Lipid phosphatase protein -3.2 0.02 LOC_Os04g33640 Glycosyl hydrolases family 17 -3.2 0.02 LOC_Os03g56260 Kinesin motor protein -3.2 0.04 LOC_Os07g05940 9-cis-epoxycarotenoid dioxygenase 1, -3.2 0.04 LOC_Os06g03670 Dehydration-responsive element-binding protein -3.1 0.00 LOC_Os06g10210 Expressed protein -3.1 0.00 LOC_Os01g61690 OsSCP5 - putative serine carboxypeptidase -3.1 0.00 LOC_Os07g37400 OsFBX257 - F-box -3.1 0.00 LOC_Os03g09070 Leucine rich protein -3.1 0.00 LOC_Os06g13870 U-box protein CMPG1 -3.1 0.01 LOC_Os01g11150 Gibberellin 2-beta-dioxygenase -3.1 0.01 LOC_Os06g11200 12-oxophytodienoate reductase -3.1 0.01 LOC_Os03g57880 Glucan endo-1,3-β-glucosidase -3.1 0.01 LOC_Os06g05470 Expressed protein -3.1 0.01 LOC_Os05g33400 Basic 7S globulin -3.1 0.01 LOC_Os02g32060 Hydrolase, NUDIX family -3.1 0.03 LOC_Os04g51460 Glycosyl hydrolases family 16, -3 0.00 LOC_Os01g09770 Expressed protein -3 0.00 LOC_Os10g25230 ZIM domain containing protein -3 0.00 LOC_Os03g12820 ATP8 -3 0.00 LOC_Os08g31860 Expressed protein -3 0.00 LOC_Os03g03550 bZIP family transcription factor -3 0.00 LOC_Os01g64620 Zinc finger, C3HC4 protein -3 0.00


14

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os01g64360 MYB family transcription factor -3 0.00 LOC_Os06g44250 Haemolysin-III -3 0.00 LOC_Os01g66010 Amino acid transporter -3 0.00 LOC_Os03g50280 GLTP domain containing protein -3 0.00 LOC_Os01g66530 ARGOS -3 0.00 LOC_Os03g32230 ZOS3-12-C2H2 zinc finger protein -3 0.01 LOC_Os09g31120 Pirin -3 0.01 LOC_Os02g45390 RING-H2 finger protein -3 0.01 LOC_Os10g39920 Expressed protein -3 0.02 LOC_Os04g49690 FERONIA receptor-like kinase -2.9 0.00 LOC_Os09g35850 RNA recognition protein -2.9 0.00 LOC_Os04g23550 Basic helix-loop-helix family protein -2.9 0.00 LOC_Os02g46650 Ubiquitin carboxyl-terminal hydrolase -2.9 0.00 LOC_Os02g52670 AP2 domain containing protein -2.9 0.00 LOC_Os01g57420 Diacylglycerol kinase -2.9 0.00 LOC_Os06g03670 Dehydration-responsive element-binding protein -2.9 0.00 LOC_Os11g10760 NBS-LRR disease resistance protein -2.9 0.00 LOC_Os03g08330 ZIM domain containing protein -2.9 0.00 LOC_Os08g37660 Plastocyanin-like protein -2.9 0.00 LOC_Os02g50110 Hypothetical protein -2.9 0.00 LOC_Os03g03200 Hydrolase -2.9 0.01 LOC_Os01g06320 MYB family transcription factor -2.9 0.01 LOC_Os03g08840 Zinc finger protein -2.9 0.01 LOC_Os08g36910 Alpha-amylase -2.9 0.02 LOC_Os01g63750 Glycine-rich protein -2.9 0.02 LOC_Os01g50350 Expressed protein -2.9 0.02 LOC_Os06g11290 12-oxophytodienoate reductase -2.9 0.02 LOC_Os07g37920 No apical meristem protein -2.8 0.00 LOC_Os12g07550 Expressed protein -2.8 0.00 LOC_Os02g51890 RNA recognition protein -2.8 0.00 LOC_Os02g40530 MYB transcription factor -2.8 0.00 LOC_Os04g48290 MATE efflux family protein -2.8 0.00 LOC_Os01g51890 Endonuclease/exonuclease/phosphatase protein -2.8 0.00 LOC_Os03g06520 Sulfate transporter -2.8 0.00 LOC_Os02g52040 Phosphate-induced protein 1 -2.8 0.00 LOC_Os04g57550 Amine oxidase -2.8 0.00 LOC_Os06g48040 Zinc finger, C3HC4 domain containing protein -2.8 0.00 LOC_Os03g49380 Lipoxygenase -2.8 0.01 LOC_Os05g50610 OsWRKY8v2 -2.8 0.01 LOC_Os07g36570 KI domain interacting kinase 1 -2.8 0.01 LOC_Os06g49890 Coiled-coil domain-containing protein 90A -2.8 0.02 LOC_Os05g50580 OsSCP30- putative aerine carboxypeptidase -2.8 0.02 LOC_Os02g08420 Cinnamoyl CoA reductase -2.8 0.02


15

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os10g35460 COBRA -2.7 0.00 LOC_Os10g39640 Expansin precursor -2.7 0.00 LOC_Os04g53990 Ethylene-responsive protein -2.7 0.00 LOC_Os01g67820 Exo70 exocyst domain containing protein -2.7 0.00 LOC_Os03g02330 AAA-type ATPase family protein -2.7 0.00 LOC_Os01g10640 Expressed protein -2.7 0.00 LOC_Os02g52010 Phosphate-induced protein 1 -2.7 0.00 LOC_Os05g30500 Expressed protein -2.7 0.00 LOC_Os05g11810 Gibberellin 2-β-dioxygenase 1 -2.7 0.00 LOC_Os11g35274 Protein kinase -2.7 0.00 LOC_Os06g14490 Calmodulin-binding heat-shock protein -2.7 0.00 LOC_Os07g44140 Cytochrome P450 -2.7 0.00 LOC_Os05g49140 MAPK, GSK3, and CLKC kinases -2.7 0.00 LOC_Os02g39000 Remorin C-terminal domain containing protein -2.7 0.00 LOC_Os01g50720 MYB transcription factor -2.7 0.00 LOC_Os01g60110 E2F-related protein -2.7 0.00 LOC_Os07g01820 OsMADS15 -2.7 0.01 LOC_Os08g38210 Transcription factor BIM2 -2.7 0.01 LOC_Os01g47680 60S ribosomal protein -2.7 0.01 LOC_Os11g39990 Patatin -2.7 0.01 LOC_Os01g54670 Coiled-coil domain-containing protein -2.7 0.01 LOC_Os01g43280 Hydroquinone glucosyltransferase -2.7 0.01 LOC_Os05g49370 DUF1645 domain containing protein -2.7 0.02 LOC_Os09g34150 NBS-LRR disease resistance protein -2.7 0.02 LOC_Os03g03790 AMP-binding protein -2.7 0.02 LOC_Os02g28170 Transferase family protein -2.7 0.03 LOC_Os07g37454 Urate anion exchanger -2.7 0.03 LOC_Os09g34110 Expressed protein -2.7 0.03 LOC_Os05g30760 Esterase -2.7 0.04 LOC_Os04g46980 cis-zeatin O-glucosyltransferase -2.7 0.04 LOC_Os10g39640 Expansin -2.6 0.00 LOC_Os01g48290 DOF zinc finger domain containing protein -2.6 0.00 LOC_Os01g55370 Expressed protein -2.6 0.00 LOC_Os06g14370 Caleosin related protein -2.6 0.00 LOC_Os02g32590 HSF-type DNA-binding protein -2.6 0.00 LOC_Os02g38120 BTBN3 - Bric-a-Brac -2.6 0.00 LOC_Os02g52210 Zinc finger, C3HC4 domain containing protein -2.6 0.00 LOC_Os02g03430 Expressed protein -2.6 0.00 LOC_Os06g44010 OsWRKY28 -2.6 0.00 LOC_Os03g28940 ZIM domain containing protein -2.6 0.00 LOC_Os03g51650 Membrane protein -2.6 0.00 LOC_Os05g37250 Cytochrome P450 -2.6 0.00 LOC_Os11g04830 Embryogenesis transmembrane protein -2.6 0.00


16

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os10g39660 Expressed protein -2.6 0.00 LOC_Os01g66510 MLO domain containing protein -2.6 0.01 LOC_Os07g01820 OsMADS15 -2.6 0.01 LOC_Os02g38392 NBS-LRR disease resistance protein -2.6 0.01 LOC_Os01g11460 Zinc finger, C3HC4 type domain containing protein -2.6 0.01 LOC_Os07g41310 COBRA -2.6 0.02 LOC_Os04g33640 Glycosyl hydrolases family 17 -2.6 0.02 LOC_Os05g48060 Phosphatidylserine synthase -2.6 0.02 LOC_Os03g03164 Homeobox protein knotted-1 -2.6 0.04 LOC_Os10g30790 Inorganic phosphate transporter -2.6 0.05 LOC_Os11g05950 Expressed protein -2.6 0.05 LOC_Os01g64110 Glycosyl hydrolase -2.6 0.01 LOC_Os05g04960 Phosphoglycerate mutase -2.5 0.00 LOC_Os12g06780 Expressed protein -2.5 0.00 LOC_Os04g33820 OsFBX132 - F-box domain containing protein -2.5 0.00 LOC_Os06g36180 Amino acid transporter -2.5 0.00 LOC_Os02g11070 3-ketoacyl-CoA synthase -2.5 0.00 LOC_Os05g16824 SHR5-receptor-like kinase -2.5 0.00 LOC_Os03g14370 ACT domain containing protein -2.5 0.00 LOC_Os05g11810 Gibberellin 2-β-dioxygenase 1 -2.5 0.00 LOC_Os01g67810 Transposon protein -2.5 0.00 LOC_Os03g19200 DNAJ heat shock protein -2.5 0.00 LOC_Os01g56300 Expressed protein -2.5 0.00 LOC_Os09g30490 EF hand family protein -2.5 0.00 LOC_Os09g25070 OsWRKY62 -2.5 0.00 LOC_Os10g33040 Receptor-like protein kinase -2.5 0.00 LOC_Os05g44060 Expressed protein -2.5 0.00 LOC_Os09g05030 Armadillo/β-catenin-like repeat family protein -2.5 0.00 LOC_Os08g34790 AMP-binding domain containing protein -2.5 0.01 LOC_Os03g57450 Calcium/calmodulin depedent protein kinases -2.5 0.01 LOC_Os08g34580 Trehalose-6-phosphate synthase -2.5 0.01 LOC_Os05g41590 Glycerol-3-phosphate dehydrogenase -2.5 0.01 LOC_Os10g36360 Expressed protein -2.5 0.01 LOC_Os01g60020 NAC domain transcription factor -2.5 0.01 LOC_Os01g02300 Receptor kinase ORK10 -2.5 0.01 LOC_Os04g43680 MYB family transcription factor -2.5 0.01 LOC_Os03g60210 DUF567 domain containing protein -2.5 0.01 LOC_Os04g41540 OsCML22-Calmodulin-related calcium sensor protein -2.5 0.01 LOC_Os03g60220 DUF567 domain containing protein -2.5 0.01 LOC_Os05g40770 Leucine-rich repeat family protein -2.5 0.01 LOC_Os09g02770 Translation initiation factor IF-2 -2.5 0.01 LOC_Os01g67010 Expressed protein -2.5 0.02 LOC_Os10g37570 OsFBDUF49 -2.5 0.02


17

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os04g37430 Lipoxygenase protein -2.5 0.02 LOC_Os07g30760 UDP-glucoronosyl and UDP-glucosyl transferase -2.5 0.02 LOC_Os07g42994 Hydrolase -2.5 0.02 LOC_Os03g03034 Flavonol synthase -2.5 0.02 LOC_Os04g32480 Zinc-finger protein -2.5 0.02 LOC_Os09g38040 Hydrolase -2.5 0.02 LOC_Os08g17500 Cinnamoyl-CoA reductase -2.5 0.03 LOC_Os01g19330 MYB family transcription factor -2.5 0.03 LOC_Os01g64170 Glycosyl hydrolases family 17 -2.5 0.03 LOC_Os01g55370 Expressed protein -2.4 0.00 LOC_Os01g21120 AP2 domain containing protein -2.4 0.00 LOC_Os01g55560 ABIL3 -2.4 0.00 LOC_Os01g63230 Growth regulator related protein -2.4 0.00 LOC_Os02g41840 DUF584 domain containing protein -2.4 0.00 LOC_Os01g72900 Abscisic stress-ripening -2.4 0.00 LOC_Os03g58740 Expressed protein -2.4 0.00 LOC_Os01g57420 Diacylglycerol kinase -2.4 0.00 LOC_Os04g44900 Lectin-like receptor kinase -2.4 0.00 LOC_Os01g56300 Expressed protein -2.4 0.00 LOC_Os07g47790 AP2 domain containing protein -2.4 0.00 LOC_Os06g51360 lysM domain containing protein -2.4 0.00 LOC_Os06g51360 LysM domain containing protein -2.4 0.00 LOC_Os01g06280 CrRLK1L homolog -2.4 0.00 LOC_Os07g47670 Hypoxia-responsive family protein -2.4 0.00 LOC_Os04g45340 Expressed protein -2.4 0.00 LOC_Os01g62190 C2H2 zinc finger protein -2.4 0.00 LOC_Os02g02730 Expressed protein -2.4 0.00 LOC_Os03g10240 DUF677 domain containing protein -2.4 0.01 LOC_Os01g55510 Dynein light chain type 1 domain containing protein -2.4 0.01 LOC_Os10g40650 Holliday junction resolvase -2.4 0.01 LOC_Os03g04890 Zinc finger family protein -2.4 0.01 LOC_Os01g54700 Retrotransposon protein -2.4 0.01 LOC_Os05g37190 ZOS5-08 - C2H2 zinc finger protein -2.4 0.01 LOC_Os07g37320 Transporter family protein -2.4 0.01 LOC_Os01g61080 OsWRKY24 -2.4 0.01 LOC_Os02g17000 OsSub14 - Putative Subtilisin homologue -2.4 0.01 LOC_Os03g55800 Cytochrome P450 -2.4 0.01 LOC_Os01g11414 Sodium/calcium exchanger protein -2.4 0.01 LOC_Os06g40500 Expressed protein -2.4 0.01 LOC_Os09g22530 Expressed protein -2.4 0.01 LOC_Os02g41510 MYB family transcription factor -2.4 0.02 LOC_Os03g19780 Expressed protein -2.4 0.02 LOC_Os07g41340 B12D protein -2.4 0.02


18

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os04g49210 Naringenin,2-oxoglutarate 3-dioxygenase -2.4 0.03 LOC_Os07g48630 Ethylene-insensitive 3 -2.4 0.03 LOC_Os05g29030 Protein phosphatase 2C -2.4 0.03 LOC_Os04g34270 Serine/threonine-protein kinase -2.4 0.03 LOC_Os01g14630 Polyprenyl synthetase -2.4 0.04 LOC_Os06g05510 Expressed protein -2.4 0.04 LOC_Os07g40290 Indole-3-acetic acid-amido synthetase -2.3 0.00 LOC_Os10g35460 COBRA -2.3 0.00 LOC_Os08g31850 Expressed protein -2.3 0.00 LOC_Os01g51920 Phosphotransferase -2.3 0.00 LOC_Os01g43950 Expressed protein -2.3 0.00 LOC_Os01g50420 STE kinases -2.3 0.00 LOC_Os01g50100 ABC transporter -2.3 0.00 LOC_Os01g60420 Expressed protein -2.3 0.00 LOC_Os03g10950 Protein phosphatase 2C -2.3 0.00 LOC_Os01g63690 Hs1 -2.3 0.00 LOC_Os01g41530 OsFBL2 - F-box domain and LRR containing protein -2.3 0.00 LOC_Os01g53920 Receptor-like protein kinase -2.3 0.00 LOC_Os12g12260 Diacylglycerol kinase 1 -2.3 0.00 LOC_Os12g12260 Diacylglycerol kinase 1 -2.3 0.00 LOC_Os07g36560 Transferase family protein -2.3 0.00 LOC_Os03g02650 LigA -2.3 0.00 LOC_Os09g23620 MYB family transcription factor -2.3 0.00 LOC_Os01g40630 Uncharacterized protein PA4923 -2.3 0.00 LOC_Os03g21140 RNA-binding zinc finger protein, -2.3 0.00 LOC_Os03g05460 Expressed protein -2.3 0.00 LOC_Os08g39850 Lipoxygenase -2.3 0.01 LOC_Os07g33910 Transporter family protein -2.3 0.01 LOC_Os07g07040 Erythrocyte binding protein 3 -2.3 0.01 LOC_Os11g02290 Expressed protein -2.3 0.01 LOC_Os01g54700 Retrotransposon protein -2.3 0.01 LOC_Os01g52530 Phosphoribosyl transferase -2.3 0.01 LOC_Os02g53200 glucan endo-1,3-β-glucosidase -2.3 0.01 LOC_Os09g19970 Expressed protein -2.3 0.01 LOC_Os03g18070 ω-3 fatty acid desaturase -2.3 0.01 LOC_Os02g43170 B-box zinc finger family protein -2.3 0.01 LOC_Os06g30710 DGCR14 -2.3 0.01 LOC_Os03g21970 Basic helix-loop-helix domain containing protein -2.3 0.01 LOC_Os06g48160 Glycosyl hydrolases family 16 -2.3 0.02 LOC_Os10g42410 Zinc-binding protein -2.3 0.02 LOC_Os04g43800 Phenylalanine ammonia-lyase -2.3 0.02 LOC_Os03g03034 Flavonol synthase/flavanone 3-hydroxylase -2.3 0.02 LOC_Os07g23120 Expressed protein -2.3 0.02


19

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os06g16640 Carboxyl-terminal peptidase -2.3 0.02 LOC_Os02g46460 Peptide transporter PTR2 -2.3 0.02 LOC_Os02g54600 STE kinases -2.3 0.03 LOC_Os10g37240 Expressed protein -2.3 0.03 LOC_Os09g38320 Phytoene synthase -2.3 0.04 LOC_Os07g17010 Chalcone synthase -2.3 0.04 LOC_Os01g57470 EF hand family protein -2.3 0.04 LOC_Os06g11090 CXE carboxylesterase -2.2 0.00 LOC_Os06g48060 ABC transporter -2.2 0.00 LOC_Os09g23084 Endoglucanase -2.2 0.00 LOC_Os02g38120 BTBN3-Bric-a-Brac -2.2 0.00 LOC_Os09g27010 Tyrosine protein kinase domain containing protein -2.2 0.00 LOC_Os04g47990 DOF zinc finger domain containing protein -2.2 0.00 LOC_Os01g72910 Abscisic stress-ripening -2.2 0.00 LOC_Os12g36920 Calmodulin binding protein -2.2 0.00 LOC_Os10g43060 Expressed protein -2.2 0.00 LOC_Os02g48210 Lectin-like protein kinase -2.2 0.00 LOC_Os04g41960 NADP-dependent oxidoreductase -2.2 0.00 LOC_Os01g54560 Trehalose synthase -2.2 0.00 LOC_Os02g57000 C2 domain containing protein -2.2 0.00 LOC_Os03g21640 Expressed protein -2.2 0.00 LOC_Os03g13300 Glutamate decarboxylase -2.2 0.00 LOC_Os01g51890 Endonuclease/exonuclease/phosphatase protein -2.2 0.00 LOC_Os02g28720 Spotted leaf 11 -2.2 0.00 LOC_Os04g54200 Diacylglycerol kinase -2.2 0.00 LOC_Os03g53020 helix-loop-helix DNA-binding protein -2.2 0.00 LOC_Os02g39910 B4-BTB-Bric-a-Brac -2.2 0.00 LOC_Os12g08090 Amino acid transporter -2.2 0.01 LOC_Os07g45100 Endonuclease/exonuclease/phosphatase protein -2.2 0.01 LOC_Os01g55510 Dynein light chain containing protein -2.2 0.01 LOC_Os07g01820 OsMADS15 -2.2 0.01 LOC_Os09g17152 OsFBX319 - F-box protein -2.2 0.01 LOC_Os03g13590 Potyvirus VPg interacting protein -2.2 0.01 LOC_Os08g04630 External NADH-ubiquinone oxidoreductase 1 -2.2 0.01 LOC_Os11g02290 Expressed protein -2.2 0.01 LOC_Os10g33050 Expressed protein -2.2 0.01 LOC_Os01g65370 MYB family transcription factor -2.2 0.01 LOC_Os01g02300 Receptor kinase ORK10 -2.2 0.01 LOC_Os12g04240 Peflin -2.2 0.01 LOC_Os09g38910 OsWAK92 - OsWAK receptor-like protein kinase -2.2 0.01 LOC_Os01g74370 DUF966 domain containing protein -2.2 0.01 LOC_Os03g26620 3-ketoacyl-CoA synthase -2.2 0.01 LOC_Os03g32220 ZOS3-11 - C2H2 zinc finger protein -2.2 0.01


20

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os02g53410 Expressed protein -2.2 0.01 LOC_Os01g62950 Ras-related protein -2.2 0.01 LOC_Os05g10310 Acid phosphatase -2.2 0.01 LOC_Os02g45170 Helix-loop-helix DNA-binding protein -2.2 0.01 LOC_Os02g51860 Dehydration response related protein -2.2 0.01 LOC_Os02g51860 Dehydration response related protein -2.2 0.01 LOC_Os01g40260 OsWRKY77 -2.2 0.01 LOC_Os01g11414 Sodium/calcium exchanger protein -2.2 0.01 LOC_Os08g34900 Pectinesterase -2.2 0.01 LOC_Os04g54620 Expressed protein -2.2 0.01 LOC_Os03g19260 Expressed protein -2.2 0.02 LOC_Os01g65510 OsFBX31 - F-box domain containing protein -2.2 0.02 LOC_Os05g50700 WRKY DNA-binding domain containing protein -2.2 0.02 LOC_Os01g31370 Glycosyltransferase -2.2 0.02 LOC_Os05g32660 Leucine-rich repeat family protein -2.2 0.02 LOC_Os03g06360 Late embryogenesis abundant protein D-34 -2.2 0.02 LOC_Os09g36500 Zinc finger, C3HC4 type domain containing protein -2.2 0.02 LOC_Os03g08930 Helix-loop-helix DNA-binding protein -2.2 0.03 LOC_Os01g43610 DUF623 domain containing protein -2.2 0.03 LOC_Os03g45730 DNA-binding protein-like -2.2 0.03 LOC_Os03g06570 IQ calmodulin-binding motif family protein -2.2 0.03 LOC_Os12g40180 Expressed protein -2.2 0.04 LOC_Os09g35790 HSF-type DNA-binding protein -2.2 0.04 LOC_Os09g27230 Brevis radix -2.2 0.04 LOC_Os06g50950 GDSL-like lipase -2.2 0.05 LOC_Os01g48290 DOF zinc finger domain containing protein -2.1 0.00 LOC_Os04g52750 Expressed protein -2.1 0.00 LOC_Os05g11840 Expressed protein -2.1 0.00 LOC_Os03g08310 ZIM domain containing protein -2.1 0.00 LOC_Os01g02290 Ser/Thr receptor-like kinase -2.1 0.00 LOC_Os09g26999 Keratin-associated protein -2.1 0.00 LOC_Os01g32380 Expressed protein -2.1 0.00 LOC_Os01g48300 ATP synthase protein I -2.1 0.00 LOC_Os04g39100 Peroxidase precursor -2.1 0.00 LOC_Os11g35120 OsWAK116 -2.1 0.00 LOC_Os01g12440 AP2 domain containing protein -2.1 0.00 LOC_Os02g11870 Expressed protein -2.1 0.00 LOC_Os01g50100 ABC transporter -2.1 0.00 LOC_Os05g37970 Universal stress protein -2.1 0.00 LOC_Os02g45450 Dehydration-responsive element-binding protein -2.1 0.00 LOC_Os01g56220 Expressed protein -2.1 0.00 LOC_Os05g41370 DUF26 kinases -2.1 0.00 LOC_Os03g21640 Expressed protein -2.1 0.00


21

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os11g35274 Protein kinase domain containing protein -2.1 0.00 LOC_Os03g56250 LRR receptor-like protein kinase -2.1 0.00 LOC_Os03g02280 DUF584 domain containing protein -2.1 0.00 LOC_Os10g40360 Proline oxidase -2.1 0.00 LOC_Os01g66940 kinase, pfkB family -2.1 0.00 LOC_Os02g21040 Aspartic proteinase nepenthesin precursor -2.1 0.00 LOC_Os04g30420 Dehydrogenase -2.1 0.01 LOC_Os09g37080 Expressed protein -2.1 0.01 LOC_Os08g34460 Tetraspanin family protein -2.1 0.01 LOC_Os05g50570 OsSCP29-putative serine carboxypeptidase homologue -2.1 0.01 LOC_Os02g47280 Growth-regulating factor -2.1 0.01 LOC_Os03g03370 Fatty acid hydroxylase -2.1 0.01 LOC_Os01g63060 Phosphatidic acid phosphatase -2.1 0.01 LOC_Os01g63060 Phosphatidic acid phosphatase -2.1 0.01 LOC_Os04g44510 GEM -2.1 0.01 LOC_Os02g43770 DUF630/DUF632 domains containing protein -2.1 0.01 LOC_Os08g01670 Invertase/pectin methylesterase inhibitor protein -2.1 0.01 LOC_Os01g09220 Transposon protein -2.1 0.01 LOC_Os09g30414 Aspartic proteinase nepenthesin-2 -2.1 0.01 LOC_Os11g04480 Peflin -2.1 0.01 LOC_Os03g02530 OsRhmbd7 - Putative Rhomboid homologue -2.1 0.01 LOC_Os03g02530 OsRhmbd7 - putative rhomboid homologue -2.1 0.01 LOC_Os04g38880 Dynein light chain protein -2.1 0.01 LOC_Os01g61620 Protein kinase family protein -2.1 0.01 LOC_Os01g61620 Protein kinase family protein -2.1 0.01 LOC_Os08g38890 Expressed protein -2.1 0.01 LOC_Os03g20090 MYB family transcription factor -2.1 0.01 LOC_Os03g46440 BTBA4 - Bric-a-Brac -2.1 0.01 LOC_Os03g15000 Zinc finger, C3HC4 type domain containing protein -2.1 0.01 LOC_Os02g06930 Protein kinase -2.1 0.01 LOC_Os02g51860 Dehydration response related protein -2.1 0.01 LOC_Os03g50160 Plastocyanin-like domain containing protein -2.1 0.01 LOC_Os01g13050 Long cell-linked locus protein -2.1 0.01 LOC_Os02g51970 Phosphate-induced protein 1 -2.1 0.01 LOC_Os08g35110 OsSAUR33 -2.1 0.01 LOC_Os07g42740 DUF1645 domain containing protein -2.1 0.01 LOC_Os03g09880 AIR12 -2.1 0.01 LOC_Os01g63930 Cytochrome P450 -2.1 0.02 LOC_Os02g32520 ERD1 protein -2.1 0.02 LOC_Os04g33640 Glycosyl hydrolases family 17 -2.1 0.02 LOC_Os04g33640 Glycosyl hydrolases family 17 -2.1 0.02 LOC_Os09g02710 CBS domain containing membrane protein -2.1 0.02 LOC_Os07g37454 Urate anion exchanger -2.1 0.03


22

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os01g46800 OsWRKY15 -2.1 0.03 LOC_Os10g38880 DUF623 domain containing protein -2.1 0.03 LOC_Os09g31130 Citrate transporter -2.1 0.03 LOC_Os10g37240 Expressed protein -2.1 0.03 LOC_Os01g64170 Glycosyl hydrolases family 17 -2.1 0.03 LOC_Os06g03520 DUF581 domain containing protein -2.1 0.03 LOC_Os04g40700 Steroid nuclear receptor -2.1 0.04 LOC_Os09g35790 HSF-type DNA-binding domain containing protein -2.1 0.04 LOC_Os06g11660 Phosphate-induced protein 1 -2.1 0.04 LOC_Os10g41130 AP2 domain containing protein -2 0.00 LOC_Os09g29940 Auxin-independent growth promoter protein -2 0.00 LOC_Os05g37450 Starch binding domain containing protein -2 0.00 LOC_Os08g29570 Pleiotropic drug resistance protein 3 -2 0.00 LOC_Os03g03510 Calcium/calmodulin depedent protein -2 0.00 LOC_Os03g13840 Senescence-associated protein -2 0.00 LOC_Os03g01740 Expressed protein -2 0.00 LOC_Os01g63230 Growth regulator related protein -2 0.00 LOC_Os11g05614 No apical meristem protein -2 0.00 LOC_Os04g47450 Expressed protein -2 0.00 LOC_Os05g35260 PB1 domain containing protein -2 0.00 LOC_Os03g14370 ACT domain containing protein -2 0.00 LOC_Os01g41660 Phosphoethanolamine/phosphocholine phosphatase -2 0.00 LOC_Os09g26780 Zinc-finger protein -2 0.00 LOC_Os03g55120 Plastocyanin-like domain containing protein -2 0.00 LOC_Os04g57690 Expressed protein -2 0.00 LOC_Os03g19400 Expressed protein -2 0.00 LOC_Os08g38270 Fasciclin domain containing protein -2 0.00 LOC_Os01g61760 Myosin heavy chain -2 0.00 LOC_Os01g41990 OsCML12 -Calmodulin-related calcium sensor protein -2 0.00 LOC_Os02g51350 OsFBK10 - F-box and kelch repeat containing protein -2 0.00 LOC_Os03g15570 STE kinases -2 0.00 LOC_Os03g08390 Expressed protein -2 0.00 LOC_Os02g31080 Hydroxyproline-rich glycoprotein family protein -2 0.00 LOC_Os09g35030 Dehydration-responsive element-binding protein -2 0.00 LOC_Os06g05130 Myristoyl-acyl carrier protein thioesterase -2 0.00 LOC_Os08g31090 Expressed protein -2 0.01 LOC_Os08g31090 Expressed protein -2 0.01 LOC_Os10g42430 Transcription factor MYC7E -2 0.01 LOC_Os10g37230 Expressed protein -2 0.01 LOC_Os01g41550 Aspartic proteinase -2 0.01 LOC_Os05g33000 E3 ubiquitin-protein ligase MGRN1 -2 0.01 LOC_Os01g63060 Phosphatidic acid phosphatase -2 0.01 LOC_Os12g17430 NBS-LRR disease resistance protein -2 0.01


23

TIGR Locus number Annotation in the TIGR database Fold (log2) P valueLOC_Os02g45780 Zinc finger, C3HC4 type domain containing protein -2 0.01 LOC_Os02g45780 Zinc finger, C3HC4 type domain containing protein -2 0.01 LOC_Os04g50216 SNARE associated Golgi protein -2 0.01 LOC_Os01g61620 Protein kinase family protein -2 0.01 LOC_Os03g19920 Expressed protein -2 0.01 LOC_Os04g46220 Ethylene-responsive transcription factor -2 0.01 LOC_Os04g24140 Ribose-5-phosphate isomerase A -2 0.01 LOC_Os01g64230 Long cell-linked locus protein -2 0.01 LOC_Os01g64230 Long cell-linked locus protein -2 0.01 LOC_Os03g01684 Retrotransposon protein -2 0.01 LOC_Os04g51450 Glycosyl hydrolases family 16 -2 0.02 LOC_Os05g46340 Expressed protein -2 0.02 LOC_Os01g62860 Oxidoreductase -2 0.02 LOC_Os09g28560 Protein phosphotase protein -2 0.02 LOC_Os07g07030 Expressed protein -2 0.02 LOC_Os07g07030 Expressed protein -2 0.02 LOC_Os01g62430 C2 domain containing protein -2 0.02 LOC_Os06g05160 Sulfate transporter -2 0.02 LOC_Os04g37430 Lipoxygenase protein -2 0.02 LOC_Os10g39010 TKL_IRAK_CrRLK1L -2 0.02 LOC_Os03g16390 Avr9/Cf-9 rapidly elicited protein -2 0.02 LOC_Os01g19940 Expressed protein -2 0.02 LOC_Os08g31250 C1-like domain containing protein -2 0.02 LOC_Os01g55500 Nucleobase-ascorbate transporter -2 0.02 LOC_Os07g48630 Ethylene-insensitive 3, -2 0.03 LOC_Os03g45280 Dehydrin -2 0.03 LOC_Os02g49950 Spotted leaf 11 -2 0.03 LOC_Os04g40700 Steroid nuclear receptor -2 0.04 LOC_Os09g31000 EF hand family protein -2 0.04 LOC_Os11g34910 Expressed protein -2 0.04 LOC_Os06g47130 C2 domain containing protein -2 0.04 LOC_Os02g39330 CHIT1 - Chitinase family protein -2 0.04 LOC_Os07g04320 Hypothetical protein -2 0.04 LOC_Os11g44340 Calmodulin binding protein -2 0.04 LOC_Os05g10650 6-phosphofructokinase -2 0.05 Fold change of the ila1 mutant compared with the wild type. Values are showed as log2 of fold changes.


24

Supplemental Table 4. Primer Sequences Used in This Article

Primer name Forward primer Reverse primer ila1 for cosegregation (a)ACACCAGCGTATGTCCTT; (c) AATCCAGATCCCCCGAATTA (b)ATGCTCCTCCCCAGCTTTTT

ila1for RT-PCR CCGATTCACAGTCAAACAT TTCTTCGTTAAATTCCTCC

Actin1 for RT-PCR GCGTCGACTCCACTCTCGC CCATGAAACAAATCCAACA

ILA1cF CCAGGTACCAGCGTTTGCAGTCGTTG CCAGGATCC TTTATGGGGATGGGTAAGA

ILA1-pDEST32 GGGGACAAGTTTGTACAAAAAAGCAGGCTACCACGGCGGTCAGGTCTC GGGGACCACTTTGTACAAGAAAGCTGGGTTTATGGGGATGGGTAAGA

ILA1-GST TGGATCCATGGACCACGGCGGTC GCTCAATGGACCTTCCCAA

ILA1-GFP GTCGACTATGGACCACGGCGGTCAG GGTACCATATGGACCTTACCCAAAAGTAAAG

ILA1R-pDEST32 GGGGACAAGTTTGTACAAAAAAGCAGGCTACCACGGCGGTCAGGTCTC GGGGACCACTTTGTACAAGAAAGCTGGGTTACGTCGAAGTTAATTTCCC

ILA1K-pDEST32 GGGGACAAGTTTGTACAAAAAAGCAGGCTGGGAAATTAACTTCGACGTA GGGGACCACTTTGTACAAGAAAGCTGGGTTTATGGGGATGGGTAAGA

ILA1-pDX2181 CCAGTCGACCACTACGACGATCAATCACG CCAGGATCCACAGCAAAGGCGCAACTCTT

ILA-pH7WGF2,0 GGGGACAAGTTTGACAAAAAAGCAGGCTACCACGGCGGTCAGGTCTC GGGGACCACTTTGTACAAGAAAGCTGGGTTTATGGGGATGGGTAAGA

IIP2-sublocalization AGGTACCATGGACCCCAGACTTGG TACTAGTCTACAGCCAGCTGCCC

IIP4-sublocalization AGGTACCATGGATAACAGCTTTGTGG TACTAGTCAGAGCTTATCCATGTCG

ILA1-pVYCE CCAGGATCCCAGCGTTTGCAGTCGTTGT CCAGGTACCATGGACCTTCCCAAAAGTAA

IIP2-pVYNE CCAGGATCCATGGACCCCAGACTTGGG CCAGGTACCCAGCCAGCTGCCCCGCAC

IIP4-pVYNE CCAGGATCCATGGATAACAGCTTTGTG CCAGGTACCGAGCTTATCCATGTCGGCGTT

IIP2-GATEWAY GGGGACAAGTTTGTACAAAAAAGCAGGCTTGGACCCCAGACTTGGGCGG GGGGACCACTTTGTACAAGAAAGCTGGGTGCAGAAGGCGGTTCAAGGCT

IIP4-GATEWAY GGGGACAAGTTTGTACAAAAAAGCAGGCTTGGATAACAGCTTTGTGGAT GGGGACCACTTTGTACAAGAAAGCTGGGTTCGGCAGCCGGAGAAGCAAAT

IIP5-RNAi CCAACTAGTGGTACCTAGCTTCTGCGTCGCTCAT CCAGAGCTCGGATCCTCCTGGCTCCCTCCGTTCT

CESA6 for qRT-PCR GAGCTTACTTGGTGGAAG AATAAACAATTGGCATCTTC

CSLC7 for qRT-PCR GAAGAAGCACAATAGGAT AGAAGAAAGTAGAAATGGAT

IRX10L for qRT-PCR GCAGGCTCATCTTATTCC TGCTGGTATCGTAGAACA

GUX1L for qRT-PCR ACTGTAACTGGAATAACC AAGACAATAGTGCTGAAG

CSLF6 for qRT-PCR GGTGTGGTGTATGATTGT CAGCCTCTCTTTCTCTCA

GT8 for qRT-PCR AGATTGATTCGTTAGGCTTC AAATGAAACCTTTATCCTATCCA

GT43 for qRT-PCR CACACGAATGGAAAATTG ACCTTCAGTATTCAATAGC


25

Primer name Forward primer Reverse primer UGA4E for qRT-PCR ATTGTCCTTGGAGGCTAG AATTCAGCATGGTTGATTG

UBQ5 for qRT-PCR ACCACTTCGACCGCCACTACT ACGCCTAAGCCTGCTGGTT

IIP1 for qRT-PCR ATACCTGTTACCAACTGTTC TCAGAGGCATACGAAGAT

IIP2 for qRT-PCR GCTCTCTCCATTTCAGTAA AATCAGGACACAACAGTT

IIP3 for qRT-PCR GCTCGTGTGAGAAAATCAGTGT AAGTTTCGTACCAACCTAACATCT

IIP4 for qRT-PCR TTGCTTTAGTCCAGTCATT TTTATTGCCAGTTCCTCC

IIP5 for qRT-PCR CATAAGATTTCAGATCACCATTG CTGCTCTTTGTGTATGTAAGT

IIP6 for qRT-PCR TCTTCATCATGTTCAATCA CGGTGCTAAAGTTCAATT

Documents

Ning ILA1 Figs suppl - Plant Cell · Complementation Test of ila1. ( A ) Constructs for complementation and biochemical assays. ( B ) Leaf angle comparison among the complementation