Nikon A1R service manual

CONFOCAL MICROSCOPE A1

Setup Manual

This manual is intended to provide setup instructions for Nikon representatives who have attended the lecture on laser safety and have been trained in setup operations. Attempts by others to set up the product may lead to accidents or equipment failure. NIKON is not responsible for personal injury or equipment damage resulting from equipment setup performed by unqualified persons. Be sure to observe the warnings and cautions described in this manual to ensure safety during setup.

M465E 13.2.Nx.13 (1/2)

WARNING

[Confidential]

i

Introduction

This setup manual is written for service personnel who is installing the Nikon Confocal Microscope A1.

To ensure correct installation, read this manual carefully before installing the product.

No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.

The contents of this manual are subject to change without notice.

This manual contains confidential information. Do not disclose this manual to a third party other than the authorized Nikon personnel.

Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you note any points that are unclear or incorrect, please contact a Nikon Service Department.

Some of the equipment described in this manual may not be included in the set you are installing.

If you intend to use any other equipment with this product, read the manual for that equipment too.

If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Microsoft, Windows, and Internet Explorer are registered trademarks of Microsoft Corporation in the U.S. and other countries. Other product and company names mentioned in this manual are trademarks or registered trademarks of their respective owners.

ii

Contents Introduction............................................................................................................................................................. i Safety Precautions .............................................................................................................................................. vii

WARNING and CAUTION Symbols Used in This Manual ......................................................................vii WARNING........................................................................................................................................ viii CAUTION........................................................................................................................................... x

Chapter 1 Laser Safety..................................................................................................................................... 1 1.1 Laser Paths ................................................................................................................................... 1

1.1.1 Laser Units..................................................................................................................... 1 1.1.2 Scan Heads ................................................................................................................... 3 1.1.3 A1-DU4 4 Detector Unit [without VAAS Detector Unit]................................................... 5 1.1.4 A1-DU4 4 Detector Unit [with VAAS Detector Unit]........................................................ 6 1.1.5 A1-DUG GaAsP Multi Detector Unit............................................................................... 7 1.1.6 A1-DUS Spectral Detector Unit...................................................................................... 8 1.1.7 Upright Microscope ........................................................................................................ 9 1.1.8 Inverted Microscope....................................................................................................... 9

1.2 Safety Labels and Components with Apertures........................................................................... 10 1.2.1 Safety Labels ............................................................................................................... 10 1.2.2 Scan Head ................................................................................................................... 11 1.2.3 Controller ..................................................................................................................... 12 1.2.4 Scan Head and Ni-E with NI-TT-E Motorized Quadrocular Tilting Tube ...................... 13 1.2.5 Scan Head and FN1 with NI-TT Quadrocular Tilting Tube........................................... 14 1.2.6 Scan Head and Ti-E..................................................................................................... 15 1.2.7 C-LU3EX 3 Laser Unit (Optional)................................................................................. 16 1.2.8 LU4A 4 Laser Unit A .................................................................................................... 17 1.2.9 LU-LR 4 Laser PS Rack 100V/120V/230V (Optional) .................................................. 18 1.2.10 A1-DU4 4 Detector Unit [without VAAS Detector Unit]................................................. 19 1.2.11 A1-DU4 4 Detector Unit [with VAAS Detector Unit]...................................................... 20 1.2.12 A1-DUG GaAsP Multi Detector Unit............................................................................. 21 1.2.13 A1-DUS Spectral Detector Unit.................................................................................... 22 1.2.14 A1-DUT Diascopic Detector Unit (Optional) ................................................................. 23

Chapter 2 System ........................................................................................................................................... 24 2.1 System Overview......................................................................................................................... 24

Chapter 3 Part Descriptions .......................................................................................................................... 34 3.1 Scan Head................................................................................................................................... 34 3.2 Controller ..................................................................................................................................... 36 3.3 Laser Units .................................................................................................................................. 38

3.3.1 C-LU3EX 3 Laser Unit (Optional)................................................................................. 38 3.3.2 LU4A 4 Laser Unit A .................................................................................................... 40 3.3.3 LU-LR 4 Laser PS Rack 100V/120V/230V (Optional) .................................................. 41

3.4 Detector Units .............................................................................................................................. 43 3.4.1 A1-DU4 4 Detector Unit [without VAAS Detector Unit]................................................. 43 3.4.2 A1-DU4 4 Detector Unit [with VAAS Detector Unit]...................................................... 45 3.4.3 A1-DUG GaAsP Multi Detector Unit............................................................................. 49 3.4.4 A1-DUS Spectral Detector Unit.................................................................................... 51 3.4.5 A1-DUT Diascopic Detector Unit (Optional) ................................................................. 52

Contents

iii

3.4.6 Transmitted Light Detector (Optional) .......................................................................... 53 3.5 Microscopes ................................................................................................................................ 54

Chapter 4 Setup Work Procedures ............................................................................................................... 58 4.1 Setup Work Procedures............................................................................................................... 58

Chapter 5 Checking Items and Tools............................................................................................................ 60 5.1 Required Items ............................................................................................................................ 60 5.2 Tools............................................................................................................................................ 60

Chapter 6 Setting up Controller .................................................................................................................... 62 6.1 Installing Controller ...................................................................................................................... 62 6.2 Installing Auxiliary Legs (for Preventing Overturn)....................................................................... 63

Chapter 7 Setting up Microscopes................................................................................................................ 64 7.1 Preparation for Attaching Scan Head to Ni-E.................................................................................. 65 7.2 Preparation for Attaching Scan Head to FN1.................................................................................. 69 7.3 Preparation for Attaching Scan Head to Ti-E .................................................................................. 72

7.3.1 Attaching Laser Safety Kit............................................................................................ 72 7.3.2 Preparation for Attaching Scan Head to Ti-E and Interlock Cable Connection ............ 74

Chapter 8 Setting up Diascopic Detector Unit ............................................................................................. 75 8.1 Attaching Diascopic Detector Unit to Microscope ........................................................................ 75

8.1.1 Ni-E or FN1 Used......................................................................................................... 77 8.1.2 Ti-E Used..................................................................................................................... 78

8.2 Attaching Diascopic Detector Unit to 4 Detector Unit................................................................... 80 8.2.1 Attaching Transmitted Light Detector........................................................................... 80 8.2.2 Attaching Light Guide and Electric Cable..................................................................... 81

Chapter 9 Setting up Detector Unit ............................................................................................................... 82 9.1 Installing 4 Detector Unit [without Spectral Detector Unit] ........................................................... 82

9.1.1 Attaching Anchor Brackets to Controller ...................................................................... 82 9.1.2 Installing 4 Detector Unit .............................................................................................. 82

9.2 Installing 4 Detector Unit [with Spectral Detector Unit] ................................................................ 83 9.2.1 Attaching Anchor Brackets to Controller and Installing Spectral Detector Unit ............ 83 9.2.2 Attaching Anchor Brackets to Spectral Detector Unit and Installing 4 Detector Unit .......... 84 9.2.3 Setting up Spectral Detector Unit................................................................................. 84

9.3 Connecting Fiber-optic Cables to Detector Unit........................................................................... 88 9.4 Attaching CLEM Hard Key........................................................................................................... 90

Chapter 10 Setting up Scan Head ................................................................................................................... 91 10.1 Attaching Ring Adapter................................................................................................................ 91 10.2 Temporarily Fixing Scan Head in Place....................................................................................... 94

10.2.1 Upright Microscope Ni-E or FN1 .................................................................................. 94 10.2.2 Inverted Microscope Ti-E ............................................................................................. 98

10.3 Connecting Fiber-optic Cables .................................................................................................... 99 10.4 Redirecting Cover of External Port ............................................................................................ 101

Chapter 11 Setting up Laser Unit .................................................................................................................. 102 11.1 Setting up C-LU3EX 3 Laser Unit .............................................................................................. 103

11.1.1 Preparation ................................................................................................................ 104

Contents

iv

11.1.2 Laser Installation........................................................................................................ 106 11.1.3 Laser Rough Adjustment 1 ........................................................................................ 109 11.1.4 Laser Rough Adjustment 2 ........................................................................................ 121 11.1.5 Final Adjustment for Ar Laser Light with Optical Fiber ............................................... 128 11.1.6 Final Adjustment for Transmitting HeNe Laser and

405-nm Laser Light through Optical Fiber.................................................................. 135 11.1.7 Adjusting AOM Controller (only when AOM Unit Is Used) ......................................... 137 11.1.8 Attaching Sheet Metal Cover ..................................................................................... 138

11.2 Setting up LU4A 4 Laser Unit A (or LU4 4 Laser Unit)............................................................... 139 11.2.1 Preparation ................................................................................................................ 140 11.2.2 Laser Installation........................................................................................................ 144 11.2.3 Laser Rough Adjustment 1 ........................................................................................ 150 11.2.4 Laser Rough Adjustment 2 ........................................................................................ 163 11.2.5 Final Adjustment for Ar Laser Light with Optical Fiber ............................................... 169 11.2.6 Final Adjustment for Other Laser Light with Optical Fiber.......................................... 174 11.2.7 Detaching AOTF Driver Remote Controller................................................................ 176 11.2.8 Operation Check ........................................................................................................ 177 11.2.9 Attaching Sheet Metal Cover ..................................................................................... 178

11.3 Setting up LU-LR 4 Laser PS Rack ........................................................................................... 179 11.3.1 Description................................................................................................................. 179 11.3.2 Mounting Laser Power Supply ................................................................................... 182 11.3.3 Details on Mounting Laser Supplies........................................................................... 188

Chapter 12 Setting up PC .............................................................................................................................. 194 12.1 Preparations .............................................................................................................................. 194

12.1.1 Tools Used................................................................................................................. 194 12.1.2 PC Performance Requirements ................................................................................. 195

12.2 Installing Giga Channel Board ................................................................................................... 197 12.2.1 Installing Giga Channel Board in PC.......................................................................... 197 12.2.2 Installing Device Driver .............................................................................................. 200 12.2.3 Tuning Memory .......................................................................................................... 206 12.2.4 Restarting PC............................................................................................................. 207

12.3 Specifying IP Address................................................................................................................ 208 12.4 Installing NIS-Elements C.......................................................................................................... 211 12.5 Installing A1 Setup Tool Software.............................................................................................. 212

12.5.1 Installing A1Tool ........................................................................................................ 213 12.5.2 Installing Application Configuration Tool .................................................................... 217 12.5.3 Installing Filter Block Setting...................................................................................... 218

12.6 Main Screen and Functions of A1Tool....................................................................................... 219 12.6.1 Main Menu ................................................................................................................. 220 12.6.2 System Parameters Management Menu.................................................................... 225 12.6.3 Advanced Window ..................................................................................................... 229

12.7 Copying and Merging System Parameter Files ......................................................................... 231 12.8 Specifying Hardware Option Information ................................................................................... 240

12.8.1 4 Laser Unit (LU4A/LU4) Settings [A1Tool before Ver. 3.22 Installed]....................... 241 12.8.2 4 Laser Unit A (LU4A) Settings [A1Tool Ver. 3.22 or Later Installed]......................... 243 12.8.3 4 Laser Unit (LU4) Settings [A1Tool Ver. 3.22 or Later Installed] .............................. 245

Contents

v

12.8.4 3 Laser Unit Settings [LU4A or LU4 Configured]........................................................ 247 12.8.5 3 Laser Unit Settings [LU4A or LU4 Unconfigured].................................................... 249 12.8.6 Detector Settings ....................................................................................................... 251 12.8.7 Other Optional Settings.............................................................................................. 253

12.9 A1 SDK Log Viewer ................................................................................................................... 254 Chapter 13 Cable Connections between Units ............................................................................................ 255

13.1 Procedures for Cable Connections............................................................................................ 255 13.2 Details of Trigger Cable ............................................................................................................. 266

13.2.1 A1 External Trigger Cable (Optional) ......................................................................... 266 Chapter 14 General Adjustment .................................................................................................................... 267

14.1 Forwarding System Parameters to Controller............................................................................ 269 14.2 Scan Head Adjustment .............................................................................................................. 272

14.2.1 Upright Microscope Ni-E or FN1 ................................................................................ 272 14.2.2 Inverted Microscope Ti-E ........................................................................................... 275

14.3 Acquiring Calibration Data Offset for Condenser Lens Position ................................................ 278 14.3.1 Explanation of Screen for Measuring and

Registering Condenser Lens Positioning Offset......................................................... 278 14.3.2 Installing Filter Cubes ................................................................................................ 282 14.3.3 Acquiring Offset Data................................................................................................. 287 14.3.4 When VAAS Detector Unit Is Equipped ..................................................................... 294

14.4 Adjustment and Checking with Fluorescent Images .................................................................. 296 14.4.1 Laser Port 2 Adjustment ............................................................................................ 296 14.4.2 Matching Image of Laser Port 2 to That of Laser Port 1 ............................................ 304

14.5 Adjusting AOM Driver with A1Tool............................................................................................. 309 14.5.1 Adjusting AOM Controller Bias and Power (Old AOM) .............................................. 309 14.5.2 Adjusting AOM Controller Bias and Power (New AOM)............................................. 312

14.6 Microscope Settings for Diascopic DIC Microscopy................................................................... 315 14.6.1 Accessories Required for Microscopes...................................................................... 315 14.6.2 Adjusting Microscope................................................................................................. 316

14.7 Measurement of Actual Wavelength.......................................................................................... 317 14.8 Accuracy Check of Spectral Detector Unit................................................................................. 326 14.9 Linear Correction of Laser Power (Manual) ............................................................................... 327 14.10 Point Simultaneous Stimulation Correction................................................................................ 330

14.10.1 Required Items........................................................................................................... 330 14.10.2 Precautions on Correction ......................................................................................... 331 14.10.3 Procedures of Correction ........................................................................................... 332 14.10.4 Performing Correction Parameter Computation and Evaluation Separately .............. 334

14.11 Setting up CLEM System Parameters ....................................................................................... 335 14.12 Merging A1 System Parameters with Configuration Data Used by NIS-Elements..................... 340

14.12.1 Creating ConfocalConfig.xml ..................................................................................... 340 (Reference) Other uses for the Application Configuration Tool ................................................. 347 14.12.2 Filter Block Setting ..................................................................................................... 349

14.13 Automatic Editing of System Parameter Files............................................................................ 355 14.14 Backing up System Parameter XML.......................................................................................... 362 14.15 Firmware Version Management................................................................................................. 363

Contents

vi

14.15.1 Firmware Version Management Menu ....................................................................... 364 14.15.2 Updating Main Control Firmware & FPGA by Using A1Tool ...................................... 367 14.15.3 Updating Other Firmware and FPGA......................................................................... 378 14.15.4 Updating Main Control Firmware & FPGA by Using A1Update ................................. 386 14.15.5 Updating DU4 Unit FPGA by Using Flash.exe........................................................... 390 14.15.6 Updating Giga Channel Board FPGA ........................................................................ 397

14.16 Dealing with System Changes................................................................................................... 398 14.16.1 Replacing PC with Other One.................................................................................... 398 14.16.2 Relocating Installation Site......................................................................................... 399 14.16.3 Adding Spectral Detector Unit (Si) ............................................................................. 399 14.16.4 Adding Lasers or Laser Unit ...................................................................................... 400 14.16.5 Adding Diascopic Detector Unit ................................................................................. 405 14.16.6 Adding Piezo Z to Microscope ................................................................................... 408 14.16.7 Removing Optional Components ............................................................................... 414

Chapter 15 Confirmation and Check Sheet Entry........................................................................................ 415 15.1 Deleting A1 Setup Tool Icon ...................................................................................................... 415 15.2 Checking Images....................................................................................................................... 415 15.3 Check Sheet .............................................................................................................................. 416

Chapter 16 Troubleshooting.......................................................................................................................... 419 Chapter 17 Maintenance ................................................................................................................................ 424 Chapter 18 Specifications.............................................................................................................................. 425

18.1 System Configurations............................................................................................................... 425 18.2 Light Sources............................................................................................................................. 426 18.3 Detectors ................................................................................................................................... 427 18.4 Scan Head................................................................................................................................. 429 18.5 Controller ................................................................................................................................... 431 18.6 Power Supply ............................................................................................................................ 432 18.7 Dimensions................................................................................................................................ 432

18.7.1 System Configurations............................................................................................... 433 18.8 Environmental Conditions.......................................................................................................... 434 18.9 Standards .................................................................................................................................. 435 18.10 Export Regulations .................................................................................................................... 435

vii

Safety Precautions

To ensure correct and safe operation, read this manual before using the product.

WARNING and CAUTION Symbols Used in This Manual

Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct installation, read the setup manual carefully and thoroughly before installing the product.

Safety instructions in this manual are marked with the following symbols to highlight their importance. For your safety, always follow the instructions marked with these symbols.

Symbol Description

WARNING

Disregarding instructions marked with this symbol may lead to serious injury or death.

CAUTION

Disregarding instructions marked with this symbol may lead to injury or property damage.

Safety Precautions

viii

! WARNING

WARNING

Use of controls or adjustments or performance of procedures other than those specified in this manual may result in hazardous radiation exposure.

1. Laser safety This laser product was designed and manufactured in compliance with the Performance Standardsfor Light-Emitting Products stipulated by the FDA in the USA and Safety of Laser Products (IEC 60825-1:2007) stipulated by the IEC. Compliance with FDA standards is in accordance with Laser Notice No. 50. Users are requested to take all appropriate safety measures specified by the preceding standards, in accordance with local laws and regulations. Use of this laser product must be supervised by a laser safety officer (LSO) and users must follow the LSOs instructions.

IEC Class 3B Laser Product

Wavelength:

400 to 700 nm (CW (continuous wave) visible laser) Beam divergence: 0.26 to 2.74 rad Maximum power or energy output:

Total power of 500 mW Max. (CW visible laser)

2. Intended product use This product is used only for microscopic observations. It must be attached to the specified microscope model. Do not use this product for any other purpose.

3. Scan head installation onto the microscope This product must be installed to a specified microscope following the installation procedure shownbelow. Failure to do so may result in blindness from inadvertent exposure to laser light emitted fromthe eyepiece section. After installing the scan head onto a microscope on a tabletop, do not move the unit around by pushing the scan head or the microscope.

Specified microscope Installation location / method

Upright microscope Ni-E, FN1 Front port of dedicated quadrocular tube

Inverted microscope Ti-E with laser safety kit Left side port via dedicated mount adapter

4. Arrangement of the PC and the remote controller The monitor, keyboard, and mouse should be placed within a distance of 1.7 m from the scan head.Secure the remote controller cable to the back of the A1 scan head using the supplied screw and place the remote controller body within a distance of 2.0 m from the scan head.

C o m p l i e s w i t h F D A p e r f o r m a n c e s t a n d a r d sf o r l a s e r p r o d u c t s e x c e p t f o rd e v i a t i o n s p u r s u a n t t o L a s e r N o t i c eN o . 5 0 , d a t e d J u n e 2 4 , 2 0 0 7 .

LASER RADIATIONAVOID EXPOSURE TO BEAMCLASS 3B LASER PRODUCTTotal Power 500mW MAX.CW 400~700nmIEC/EN60825-1: 2007

Safety Precautions

ix

WARNING

5. Pre-operation check items Before using the system, always confirm the following.

An objective or a cap is in place on each of the nosepiece sockets. A dia-illumination lamphouse or an epi-illumination lamphouse is attached. There are no mirrors or other highly reflective pieces of metal in the area surrounding the

lens aperture.

The provided cap is in place on the rear port of the quadrocular tube when the upright microscope Ni-E or FN1 is used without the DSC zooming port for quadrocular tube.

A cap or a TV camera is in place on the right side port when the inverted microscope Ti-Eis used.

6. Never attempt to disassemble This product uses lasers. Use only in the configuration set up by a Nikon representative. To ensure safety, never attempt to remove any part of the system. Before beginning operations, confirm that all of the parts listed below are in place. Removing any

part during use may result in unintended exposure of eyes or skin to laser light. Be sure to follow the LSOs instructions when replacing an objective.

A1 scan head, eyepiece tube, nosepiece, objective, epi-fluorescence attachment, stage, lamphouse, a cap on the rear port of the quadrocular tube for the upright microscope Ni-E or FN1 (when the dedicated DSC zooming port for quadrocular tube is not used), and a cap or a TV camera on the right side port of the inverted microscope Ti-E

7. Never look into the laser beam When the laser is turned on, a powerful beam of light is emitted through the objective and the external port. Never look into this beam or reflected beams. Always wear laser-protection goggles ifyou are moving within the Nominal Ocular Hazard Distance (NOHD)*1 while the laser is on. To prevent reflection of the laser beam emitted from the objective and the external port and resulting inadvertent exposure of eyes or skin to laser light, never place reflective objects on the stage or in the diascopic optical path.*2

*1: NOHD (Nominal Ocular Hazard Distance): 1.60 m

*2: MPE (Maximum Permissible Exposure) at the cornea for a laser beam: 1 W/m2 (when the CW visible laser of 400 to 700 nm is used)

MPE (Maximum Permissible Exposure) at the skin for a laser beam: 2000 W/m2 (when the CW visible laser of 400 to 700 nm is used)

8. Manuals and accessories provided by the distributor Deliver the all manuals and accessories used in this laser product and provided by the distributor tothe user.

Safety Precautions

x

! CAUTION

CAUTION

1. Setting up the system Only trained service personnel should set up this system. Assigning this task to other personnel may result in improper setup, impairing system performance and exposing eyes or skin to laser beam, resulting in injury.

2. Do not disassemble Disassembling the system may result in electric shock or other hazards. Never attempt to disassemble any piece of equipment included in the system. In particular, disassembling any of the components when the laser is on may cause the laser beamto be improperly emitted from the system. If you discover that the system is not functioning properly,immediately stop use, turn off the power switches to all pieces of equipment, disconnect the power cables from the outlets, and contact your nearest Nikon representative.

3. Power supply ratings and power supply cords Before connecting power cords to wall outlets, check your power-line ratings to make sure that the input voltage and current-carrying capacity requirements of equipment are met. Use of a non-conforming power line may result in equipment malfunction, failure, or fire. Always use power cords that satisfy local safety regulations. Always use three-pole grounded outlets.

4. Cable connections Always turn off the power switches to all equipment before connecting or disconnecting cables. To prevent wires from breaking, do not pull cables by the sheath; instead, firmly grasp the plug whenconnecting or disconnecting a cable.

5. Countermeasures against problems If you notice that the system is not working properly, quickly abort the software on the PC, turn off allpower switches, and disconnect power cords from the AC outlets. When the LU-LR 4 laser PS rackhas a problem, disconnect both of two power cords from the AC outlets.

6. Avoid contact with water Exposure of any system equipment to liquids may cause a short circuit, resulting in malfunction or abnormal heating. If water is splashed onto, immediately turn off the power switches for the equipment, and then wipe off moisture with a piece of dry cloth or something similar. If water entersthe equipment, stop using this system and remove the power cord from the receptacle, and contactyour nearest Nikon representative.

7. Do not place any objects on system equipment Never place any objects (heavy ones in particular) on the equipment, except for mounting the laserunit onto the 4 laser PS rack (see the next caution item 8). Object can injure the human body when dropped. A heavy object may cause the deformation, breakage or malfunction of the equipment.

8. The laser unit and LU-LR 4 laser PS rack setup The laser unit is designed to be used on top of the LU-LR 4 laser PS rack. The laser unit is so heavythat it can injure the human body when dropped. To avoid injury, be careful not to accidentally pushthe laser unit off the LU-LR 4 laser PS rack.

Safety Precautions

xi

CAUTION

9. Handling 1. Handle with care

This system is a precision optical instrument. Make sure that the system is not subject to shock;handle equipment gently. Also be sure to handle cables with care. Optical-fiber cables, in particular, must not be bent or pulled with excessive force to prevent failure, malfunction, or break. Both the microscope and the scan head are precisely adjusted. Subjecting these instruments toshock or forced transfer may result in the unevenness of acquired images.

2. Installation conditions

To ensure safe and reliable operations, check the following conditions when installing the system:

To prevent equipment from falling and to avoid eventual failure, install each component of thesystem on a level surface.

To prevent performance degradation caused by dust or other foreign particles, use the systemin a dirt-free environment.

Use the system in the environment specified in Chapter 18, Specifications. To prevent condensation and failure, never attempt to use the system under conditions of high temperature and humidity.

Make sure that the microscope and the scan head are installed on a solid top, such as a vibration-free table or a vibration-reduced steel sheet, since vibrations may degrade image quality.

To prevent degradation, malfunction, or failure of your laser unit and PC, always use power supply that is free from electric noise or sudden voltage fluctuations.

To prevent failure, do not block the air flow of the cooling fan and do not place any obstacles near the cooling fans of the controller, PC, LU-LR 4 laser PS rack, or laser units. If a particularpiece of equipment is installed close to a wall, be sure to provide at least 15 cm of space between the wall and the equipment.

3. Transporting

Do not push the laser unit when transferring the LU-LR 4 laser PS rack with laser unit mounted.Doing so may have the laser unit fall off the LU-LR 4 laser PS rack. Be sure to push the LU-LR 4laser PS rack when transferring.

4. Cleaning

Use a soft dry cloth to clean painted, plastic, and printed parts. If necessary, wipe these parts with a cloth moistened with diluted neutral detergent, then wipe off the moisture with a soft dry cloth. To prevent discoloration or deformation of painted or plastic parts or removal of the printedletters/figures, do not use organic solvents such as paint thinner or alcohol.

5. Other precautions

Be sure to follow the instructions specified in this manual and in the manual supplied with the software. Failure to do so may result in impaired system performance, malfunction, failure, or unexpected hazards.

Safety Precautions

xii

1

Laser Safety 1

1.1 Laser Paths

1.1.1 Laser Units

Figure 1.1-1

(1) L1 (See the table below.) (4) L4 (See the table below.) (7) Laser outlet (single-mode fiber-optic cable)

(2) L2 (See the table below.) (5) Motorized shutter (8) Wavelength selection filter

(3) L3 (See the table below.) (6) Laser coupling mirror (9) AOTF brightness adjustment part Table 1.1-1

L1 L2 L3 L4

C-LU3EX 3 laser unit *1, 3

405-nm laser *2

440-nm laser *2

638-nm laser *2

Ar laser (single line or multiple lines, 488 nm only) or 488-nm solid-state laser

G-HeNe laser 561-nm solid-state laser 594-nm solid-state laser *3, 4

---

LU4A

4 laser unit A *5 638-nm laser

405-nm laser

440-nm laser *3

Ar laser (single line or multiple lines) or 488-nm solid-state laser

G-HeNe laser 561-nm solid-state laser 594-nm solid-state laser *3

(6)

(1)

(7)

(4)

(3) (2)

(5)

(6)

(2)

(3)

(1)

(5)(8)

(6)

(7)

LU4A 4 laser unit A C-LU3EX 3 laser unit

(5)

(9)

1 Laser Safety >> 1.1 Laser Paths

2

*1: Connect this unit to the laser port 2 of the scan head. See Section 1.1.2, Scan Heads. *2: Use a Melles Griot product only. *3: Only manual mode is allowed for filter setting. *4: The filters in the C-LU3EX 3 laser unit need to be changed. *5: Contact your nearest Nikon representative for the setups other than shown in the table.

The 647-nm laser (installed when the LU4A 4 laser unit A with the laser beam path switching unit attached is shared between the N-STORM system and the A1/A1R system) cannot be used for the A1/A1R system.


3

1.1.2 Scan Heads

Scan Head (A1-SHS A1 Scan Head & Controller)

Figure 1.1-2

(1) Laser port 1 (Single-mode fiber-optic cable)


(3) Collimator lens

(4) 1st dichroic mirror

(5) Scanner mirror

(6) Scanner lens

(7) Condenser lens

(8) Pinhole

(9) Fluorescence outlet to the 4 detector unit or GaAsP multi detector unit (High-efficiency, multi-mode fiber-optic cable)

(10) External port for optional use (FC connector)

(11) Fluorescence outlet to the spectral detector unit (High-efficiency, multi-mode fiber-optic cable)

(12) Fluorescence outlet to the 4 detector unit (High-efficiency, multi-mode fiber-optic cable for VAAS)

(13) VAAS pinhole

(8)

(4) (3)(7)

(6)

(1)

(5)

(2)

(9)

(10)

(11)

(12)

(13)


4

High-speed Scan Head (A1-SHR A1 R Scan Head & Controller / A1-SHRB A1 R Scan Head & Controller)

Figure 1.1-3



(3) Collimator lens

(4) 1st dichroic mirror

(5) Scanner mirror (Galvano pair)

(6) Scanner lens

(7) Condenser lens

(8) Pinhole

(9) Fluorescence outlet to the 4 detector unit or GaAsP multi detector unit (High-efficiency, multi-mode fiber-optic cable)

(10) External port for optional use (FC connector)

(11) Fluorescence outlet to the spectral detector unit (High-efficiency, multi-mode fiber-optic cable)

(12) Fluorescence outlet to the 4 detector unit (High-efficiency, multi-mode fiber-optic cable for VAAS)

(13) VAAS pinhole

(14) Optical path switch mirror

(15) Scanner mirror (High-speed pair)

(15)

(6)

(4) (3)(7)

(1)

(14)

(2)

(9)

(10)

(11)

(5)

(8)

(12)

(13)


5

1.1.3 A1-DU4 4 Detector Unit [without VAAS Detector Unit*]

PM

T3

WH

EE

L3 FB

PM

T4

PM

T2

WH

EE

L2 FB

PM

T1

WH

EE

L1 FB

Figure 1.1-4

(1) Collimator lens

(2) Shutter

(3) Filter cube for fluorescence separation

(4) Condenser lens

(5) Photodetector (PMT)

(6) Filter wheel

(7) Fluorescence inlet from the scan head (High-efficiency, multi-mode fiber-optic cable)

(8) Fiber-optic cable for the diascopic detector unit (optional)

(9) Transmitted light detector (optional) * The VAAS detector unit is optional.

(7)

(8) (9)

(6)

(3)

(6)

(3)

(1)

(4)

(5)

(6)

(2)

(3)

(5)

(4)

(5)

(5)


6

1.1.4 A1-DU4 4 Detector Unit [with VAAS Detector Unit*]

PM

T3

WH

EE

L3 FB

PM

T4

PM

T2

WH

EE

L2 FB

PM

T1

WH

EE

L1 FB

PM

T3

WH

EE

L6 FB

PM

T4

PM

T2

WH

EE

L5 FB

PM

T1

WH

EE

L4 FB

Figure 1.1-5

(1) Standard and VAAS1 (In-focus) detectors

(2) VAAS2 (Near-focus) detector

(3) Collimator lens

(4) Optical path switching

(5) Shutter


(7) Condenser lens


(9) Filter wheel

(10) Fluorescence inlet from the scan head (Standard: High-efficiency, multi-mode fiber-optic cable)

(11) Fluorescence inlet from the scan head (VAAS1: High-efficiency, multi-mode fiber-optic cable for In-focus detection)

(12) Fluorescence inlet from the scan head (VAAS2: High-efficiency, multi-mode fiber-optic cable for Near-focus detection)


(14) Transmitted light detector (optional)

* The VAAS detector unit is optional.

(10)

(14)

(5)

(6)

(5)

(6)

(8)

(9) (8)

(7)

(8)

(8)

(9)

(6)

(7)

(6)

(12)

(11)

(7) (8) (8)

(6)

(6)

(8)

(8)

(9)

(9)

(2) (3) (9) (1) (4) (3) (13)


7

1.1.5 A1-DUG GaAsP Multi Detector Unit

Figure 1.1-6

(1) Collimator lens

(2) Shutter


(4) Condenser lens


(6) Photodetector (GaAsP PMT)

(7) Filter wheel



(10) Transmitted light detector (optional) Note: The VAAS detector unit cannot be incorporated.

(8)

(7)

(3)

(4)

(2)

(6)

(7)

(3)

(7)

(3)

(10) (9) (1)

(5)

(4)

(6)

(5)


8

1.1.6 A1-DUS Spectral Detector Unit

Figure 1.1-7


(2) Collimator lens

(3) Total reflection mirror

(4) Polarized beam splitter

(5) Fresnel rhomb wave plate

(6) Diffraction grating

(7) Concave mirror

(8) Optical detector array

(5)

(8)

(7)

(1)

(4)

(3)

(6)

(2)


9

1.1.7 Upright Microscope

Figure 1.1-8

1.1.8 Inverted Microscope

Figure 1.1-9

Laser inlet

Fiber-optic cable (high-efficiency, multi-mode)

Fiber-optic cable

4 detector unit or GaAsP multi detector unit

Diascopic detector unit

Laser beam

Scan headFiber-optic cable

(single-mode)

Spectral detector unit

External port for optional use (laser aperture)

Fiber-optic cable (high-efficiency, multi-mode)

Fiber-optic cable

4 detector unit or GaAsP multi detector unit

Diascopic detector unit

Laser beam

Scan head


External port for optional use (laser aperture)

Laser inlet

Fiber-optic cable (single-mode)

1 Laser Safety >> 1.2 Safety Labels and Components with Apertures

10

1.2 Safety Labels and Components with Apertures

1.2.1 Safety Labels

(1) IEC warning label Laser

hazard symbol

(2) IEC explanatory label



(3) Safety standard label

Figure 1.2-1 Figure 1.2-2 Figure 1.2-3

(4) IEC aperture label


(6) IEC caution label

Class 3B areas CAUTION CLASS 3BLASER RADIATION WHEN OPENAVOID EXPOSURE TO THE BEAM

VORSICHT LASERSTRAHLUNGKLASSE 3B,WENN ABDECKUNG GEFFNETNICHT DEM STRAHL AUSSETZEN


(7) Caution label

(8) IEC caution label

Class 4 areas

(9) Caution label



11

1.2.2 Scan Head


(6) IEC caution label Class 3B areas CAUTION CLASS 3BLASER RADIATION WHEN OPENAVOID EXPOSURE TO THE BEAM


(9) Caution label


Figure 1.2-13 A. Component with aperture

B. Component with aperture

Figure 1.2-14 Figure 1.2-15

* For the laser path inside the scan head, see Section 1.1.2, Scan Heads.

Aperture

Aperture

(6)

(6)

(6)*A

B

B

(9)

(6)

(6)

(5) Aperture


12

1.2.3 Controller

(1) IEC warning label Laser

hazard symbol

(2) IEC explanatory label



(3) Safety standard label




Figure 1.2-19

(1)

(2)

(3)


13

1.2.4 Scan Head and Ni-E with NI-TT-E Motorized Quadrocular Tilting Tube


Figure 1.2-20

(6) IEC caution label Class 3B areas

CAUTION CLASS 3BLASER RADIATION WHEN OPENAVOID EXPOSURE TO THE BEAM


Figure 1.2-21

Figure 1.2-22

A. Component with aperture


C. Component with aperture

D. Component with aperture

Figure 1.2-23 Figure 1.2-24 Figure 1.2-25 Figure 1.2-26

(6)(4)

A

B

C D

Aperture Aperture Aperture

Aperture

(6) (6) (6)

Aperture


14

1.2.5 Scan Head and FN1 with NI-TT Quadrocular Tilting Tube


Figure 1.2-27

(6) IEC caution label Class 3B areas

CAUTION CLASS 3BLASER RADIATION WHEN OPENAVOID EXPOSURE TO THE BEAM


Figure 1.2-28

Figure 1.2-29

A. Component with aperture


C. Component with aperture

D. Component with aperture

Figure 1.2-30 Figure 1.2-31 Figure 1.2-32 Figure 1.2-33

(4)

B

Aperture Aperture Aperture

Aperture

(6)

A

C

D

Aperture

(6) (6) (6)


15

1.2.6 Scan Head and Ti-E

(1) IEC warning label Laser hazard symbol

(4) IEC aperture label (5) IEC aperture label




Figure 1.2-37

Figure 1.2-38 A. Component with aperture



(1)

(4)

B

Aperture

(6)(6)

Aperture

A

(6)*(5)*

Aperture of analyzer point(6)

Objective aperture

(6)

(5)

* Another pieces of labels (5) and (6) are in the same location on the other side of the microscope.


16

1.2.7 C-LU3EX 3 Laser Unit (Optional)


(8) IEC caution label Class 4 areas


Figure 1.2-43

* For the laser path inside the laser unit, see Section 1.1.1, Laser Units. Affix the laser class 4 label supplied with the laser unit on this label (the factory affixed laser class 3B label) at the setup.

(1)

(8) *


17

1.2.8 LU4A 4 Laser Unit A


(8) IEC caution label Class 4 areas


Figure 1.2-46

* For the laser path inside the laser unit, see Section 1.1.1, Laser Units.

(1) (8) *


18

1.2.9 LU-LR 4 Laser PS Rack 100V/120V/230V (Optional)

(7) Caution label

Figure 1.2-47

Figure 1.2-48

(7)

Front view

Rear view


19

1.2.10 A1-DU4 4 Detector Unit [without VAAS Detector Unit]



Figure 1.2-49

Figure 1.2-50

For the laser path inside the detector unit, see Section 1.1.3, A1-DU4 4 Detector Unit [without VAAS Detector Unit].

(6) (6)

(6)


20

1.2.11 A1-DU4 4 Detector Unit [with VAAS Detector Unit]



Figure 1.2-51

Figure 1.2-52

For the laser path inside the detector unit, see Section 1.1.4, A1-DU4 4 Detector Unit [with VAAS Detector Unit].

(6) (6)

(6)

(6)


21

1.2.12 A1-DUG GaAsP Multi Detector Unit



Figure 1.2-53

Figure 1.2-54

For the laser path inside the GaAsP multi detector unit, refer to Section 1.1.5, A1-DUG GaAsP Multi Detector Unit.

(6)

(6)


22

1.2.13 A1-DUS Spectral Detector Unit



Figure 1.2-55

Figure 1.2-56 For the laser path inside the spectral detector unit, see Section 1.1.6, A1-DUS Spectral Detector Unit.

(6)


23

1.2.14 A1-DUT Diascopic Detector Unit (Optional)



Figure 1.2-57

1 2 3 4EXT IN

15

Figure 1.2-58

(6)

(6)

24

System 2

2.1 System Overview

LU4-A1 connection kit A

LU-LR 4 laser PS rack 100V/120V/230V

LU4A 4 laser unit A

C1-AOM AOM unit

A1-SHS A1 scan head & controller

A1/A1R scan head & controller

Laser units

Microscopes

Use the standard (A1-SHS) or optional (A1-SHR) scan head & controller.

A1-LU3EX AOM connection cable

C1-AREX EX changer

C-LU3EX 3 laser unit *1

A1-SHR A1 R scan head & controller /

A1-SHRB A1 R scan head & controller

ControllerScan head

Figure 2.1-1

LU-SF single-mode fiber

Ni-E FN1

Controller Scan head

*1: Connect this unit to the laser port 2 of the scan head.

(1)

NI-TT-E motorized quadrocular tilting tube

A1-TII interlock cable A1-U-TT FN1/Ni adapter

(5) (1)

NI-TT quadrocular tilting tube A1-TII interlock cable A1-U-TT FN1/Ni adapter Remote focus drive NIKRFK (Prior) *2 Remote focus adapter kit for FN1

NIKFN1MA (Prior) *2

(5)

(1)

(2)

(3)(4)(6)

A1-TI Ti adapter set A1-TII interlock cable

Ti-E with laser safety kit

2 System >> 2.1 System Overview

25

*2: Required only for acquiring the Z-stack image. *3: Required only for acquiring the high-speed Z-stack image. A piezo adapter is necessary for attaching this piezo positioner. *4: Required only for acquiring the high-speed Z-stack image. No piezo adapter is necessary for attaching this piezo

positioner.

Filter cube

A1-DU4 4 detector unit

Host PC & operation software NIS-Elements C

(1) C1-TD-J transmit adapter J

(2) A1-DUT-TI DUT mounting adapter

A1-DUT diascopic detector unit

A1 1STDM EX457 A1 1STDM EX405/488/543 A1 1STDM EX457/514/561 A1 1STDM EX440/514/594

NIS-A CLEM *6

A1-TIP piezo stage connection cable

Figure 2.1-2

A1-DUS spectral detector unit A1-F 450/50 A1-F 482/35 A1-F 525/50 A1-F 595/50 700/75 A1-F 540/30 A1-F 515/30 A1-F 585/65 700/75 A1-F 550/49 594LP

(3)

Optional 1st dichroic mirrors

PZT single nosepiece for Ti (4)

Detector units

A1-DUV VAAS detector set *5

A1-DUV VAAS detector set *5

A1-DUG GaAsP multi detector unit *5

(5)

(6)

200-m objective piezo positioner (MCL) *3

D-PA6 piezo adapter CFI60 D-PA7 piezo adapter CFI75 450-m objective piezo positioner

(MCL) *4

100-m stage piezo positioner (MCL)


26

*5: The A1-DUV VAAS detector set is incorporated into the scan head and the A1-DU4 4 detector unit. It cannot be incorporated into the A1-DUG GaAsP multi detector unit.

*6: The A1 CLEM hard key of the NIS-A CLEM is to be attached to the controller.


27

Table 2.1-1: System overview (sheet 1 of 5) Unit Name Code No. Main Component

A1-SHS A1 scan head & controller: Scan head, controller, additional laser port, detection port, A1-RC remote controller, PC Giga channel board, and A1 1STDM EX

(1) 405/488 (2) 405/488/561

(3) 405/488/561/638 (4) 457/514

(5) 405/488/543/638 (6) BS 20/80

MHA50000

- Head (x 1) - Controller (x 1) - Remote controller (x 1) - PC interface board (x 1) - Single-mode fiber-optic cable (x 1) - LAN cable (x 1) - Fiber-optic cables for communication (x 2) - Head controlling cable (x 1) - Scanner cable (x 1) - System constant setup CD-ROM (x 1)

A1-SHR A1 R scan head & controller: High-speed scan head, controller, additional laser port, detection port, A1-RC remote controller, PC Giga channel board, and A1 1STDM EX

(1) 405/488 (2) 405/488/561

(3) 405/488/561/638 (4) 457/514

(5) 405/488/543/638 (6) BS 20/80

MHA50100


Scan head & controller units

A1-SHRB A1 R scan head & controller:High-speed scan head, controller, additional laser port, detection port, A1-RC remote controller, PC Giga channel board, and A1 1STDM EX

(1) 405/488 (2) 405/488/561

(3) 405/488/561/638 (4) 457/514

(5) 405/488/543/638 (6) BS 20/80

MHA50110


A1 1STDM EX457 MHE56000 - Dichroic mirror (x 1) - Clamp (x 1) - Mirror frame (x 1)

A1 1STDM EX405/488/543 MHE56010 - Dichroic mirror (x 1) - Clamp (x 1) - Mirror frame (x 1)


1st dichroic mirrors


A1-DU4 4 detector unit MHE50000

- Main body (x 1) - Fiber-optic cable for fluorescence (x 1)- Anchor brackets (x 4) - Electric cable (x 1) - System constant setup CD-ROM (x 1)

Detector units

A1-DUG GaAsP multi detector unit *1 MHE50000

- Main body (x 1) - Fiber-optic cable for fluorescence (x 1)- Anchor brackets (x 4) - Electric cable (x 1) - System constant setup CD-ROM (x 1)


28


A1-DUT diascopic detector unit MHE50300

- Main body (optical path switching) (x 1)- Light guide (x 1) - PMT unit (x 1) - Electric board (x 1) - Electric board mounting adapter (x 1) - Electric cables (x 3)

C1-TD-J transmit adapter J (for Ni-E or FN1)

MHE47700 - Adapter (x 1)

Diascopic detector & adapters

A1-DUT-TI DUT mounting adapter (for Ti-E)

MHE50330 - Adapter (x 1)


A1-DUS spectral detector unit MHE50100

- Main body (x 1) - Capture board (x 1) - Electric cable (x 1) - Anchor brackets (x 4) - Supporting legs for controller (x 4) - System constant setup CD-ROM (x 1)

VAAS detector set *1

A1-DUV VAAS detector set MHE50200

- Main body (optical path switching) (x 1)- Main body (detector) (x 1) - Main body (detection box) (x 1) - Electric boards (x 3) - Electric board mounting panel (x 1) - Electric cables (x 5)

CLEM NIS-A CLEM MHS50100 - Hard key (x 1)

LU4A 4 laser unit A MHF41402

- Main body (x 1) - 561 laser adapter (x 1) - 561 laser adapter cover (x 1) - AOTF data sheet (x 1)

LU4-A1 connection kit A MHF41421 - Cables (x 3) - Shorting pin jack (x 1) - Rectangular shorting connector (x 1)

LU-LR 4 laser PS rack 100V MHF44000 - Main body (x 1) - Key switches (x 2) - DPSS cables (x 2)



C-LU3EX 3 laser unit *2 MHF42305 - Main body (x 1) - Controller connecting cable (x 1) *3 - Laser wavelength indicator label (x 1)

A1-LU3EX AOM connection cable MXA22096 - Cables (x 2)

C1-AOM AOM unit MHE48000

- AOM (x 2) - AOM Controller (x 1) - AOM cable (x 1) - PC cable (x 1) *3 - RF cable (x 1) - SYNC cable (x 1) *3 - SYNC panel (x 1) *3 - Cover boards (x 2)

C1-AREX EX changer MHF43000 - Main body (x 1) - Display board (x 1)

Laser units & accessories

LU-SF single-mode fiber *4 MHF44010 - Single-mode fiber-optic cable (x 1)


29


Laser See Table 2.1-2 ---

Filter cube See Table 2.1-3 ---

Host PC ---

NIS-Elements C MHS50000 ---

A1-TIP piezo stage connection cable MXA22093 - Electric cable (x 1)

PC & etc.

A1 external trigger cable MXA22108 - Electric cable (x 1) *1: The A1-DUV VAAS detector set cannot be incorporated into the A1-DUG GaAsP multi detector unit. *2: Connect this unit to the laser port 2 of the scan head by using the supplied fiber in case this unit is the only

laser unit in the system. Use the LU-SF single-mode fiber in case this unit and the LU4A 4 laser unit A are both used in the system.

*3: Not to be used in A1. *4: This cable is required only when both the C-LU3EX 3 laser unit and the LU4A 4 laser unit A are used in the

system.


30


NI-TT-E motorized quadrocular tilting tube

MBB94510 - Main body (x 1)

NI-RPZ-E motorized DSC zooming port for quadrocular tube

MBB96710 - Main body (x 1) - Lubber sticker (x 1)

NI-RPZ DSC zooming port for quadrocular tube


NI-FLT6-E motorized epi-fluorescence cube turret

MBE94300

- Main body (x 1) - UV light shield (x 1) - Light shielding tube (x 1) - Label (x 1)

NI-FLT6-I intelligent epi-fluorescence cube turret

MBE94400


NI-FLEI epi-fluorescence attachment for Ni

MBE94100 - Main body (x 1) - Label (x 1)

NI-BAW-E motorized barrier filter wheel

MBE94620 - Main body (x 1) - Wavelength label (x 1) - Dust-proof cap (x 1)

NI-PAU Ni photoactivation unit

MBE94800 - Main body (x 1) - Lens for stage Z drive (x 1)

A1-U-TT FN1/Ni adapter

MHV55070

- Pillar with screws (x 1 set) - Cable clamps (x 4) - Attaching brackets (x 2) - Fixing parts (x 4) - Dedicated feet (x 4) - Mount adapter (x 1) - Ring adapters (x 7) - Support bar (x 1) - Head spacers (x 2)

NI-AST analyzer slider stopper

MXA22120 - Main body (x 1) - Safety label (x 1)

A1-TII interlock cable MXA22094 - Electric cable (x 1) (Applicable to A1/C2)

D-PA6 piezo adapter CFI60

MXB22357 - Main body (x 1) - Attaching tool (x 1)

Mount adapters for microscope (1 of 2)

For Ni-E




31


NI-TT quadrocular tilting tube

MBB94521 - Main body (x 1)

NI-RPZ DSC zooming port for quadrocular tube


NI-FLT6 epi-fluorescence cube turret

MBE94500


NI-FLEI epi-fluorescence attachment for Ni

MBE94100 - Main body (x 1) - Label (x 1)

A1-U-TT FN1/Ni adapter

MHV55070

- Pillar with screws (x 1 set) - Cable clamps (x 4) - Attaching brackets (x 2) - Fixing parts (x 4) - Dedicated feet (x 4) - Mount adapter (x 1) - Ring adapters (x 7) - Support bar (x 1) - Head spacers (x 2)

NI-AST analyzer slider stopper

MXA22120 - Main body (x 1) - Safety label (x 1)

A1-TII interlock cable MXA22094 - Electric cable (x 1) (Applicable to A1/C2)



For FN1



A1-TI Ti adapter set MHV55010 - Mount adapter (x 1) - Ring adapter (x 1)

A1-TII interlock cable MXA22094 - Electric cable (x 1)

Mount adapters for microscope (2 of 3)

For Ti-E

PZT single nosepiece for Ti

MXB22318 - Main body (x 1)


32

Table 2.1-2: Recommended lasers Type Product Specifications

CW violet diode laser Manufacturer: Melles Griot *1 Head: 56 RCS series 56 RCS/S 2780 Power supply: 56 IMA series (56 IMA021)

400 to 410 nm (36 mW)

Manufacturer: Melles Griot *1 Head: 56 RCS series 56 RCS/S 2781 Power supply: 56 IMA series (56 IMA021)

440 to 445 nm (20 mW)

Manufacturer: Coherent *2, 3 Head: CUBE series (CUBE405) Power supply: CUBE series

4055 nm (50, 100 mW)


4455 nm (40 mW)

CW air-cooled argon ion laser

Manufacturer: Melles Griot Head: IMA101 series IMA 101040 ALS, IMA 101065 ALSPower supply: 300 series

457 to 514 nm (40, 65 mW)

Manufacturer: Spectra Physics Head: 163 series (163-02, 163-12) Power supply: 263C series

457 to 514 nm (25, 40 mW)

CW blue solid-state laser Manufacturer: Melles Griot *2 Head: 85BCD series (85BCD010, 85BCD020, 85BCD030)Power supply: 85BCD series

4880.5 nm (10, 20, 30 mW)

Manufacturer: Coherent *2 Head: Sapphire 488 LP series Power supply: Sapphire LP USB CDRH Driver

4882 nm (10, 20, 25, 30, 50, 75, 100, 150 mW)

Manufacturer: Coherent *2, 3 Head: Sapphire 488HP series (with heat sink) Power supply: CDRH model

4882 nm (100, 200 mW)

CW green solid-state laser

Manufacturer: Coherent *5 Head: Sapphire 514 LP series Power supply: Sapphire CDRH Driver

5142 nm (20, 50, 75, 100 mW)

Manufacturer: Coherent *5 Head: Sapphire 532 LP series Power supply: Sapphire CDRH Driver

5322 nm (20, 50, 75, 100 mW)

CW green helium-neon laser

Manufacturer: Melles Griot Head: 05LGP193 Power supply: 05LPL951-065: for 100V

05LPL915-065: for 115/230V

543.5 nm (1 mW)

CW yellow solid-state laser Manufacturer: Melles Griot *1 Head: 85YCA series (85YCA010, 85YCA020, 85YCA025)Power supply: 85YCA series (accessory)

5610.5 nm (10, 20, 25 mW)

Manufacturer: Coherent *2 Head: Sapphire 561LP series Power supply: Sapphire LP USB CDRH Driver

5602 nm (20, 50, 75, 100 mW)

CW orange solid-state laser

Manufacturer: Spectra-Physics *2, 4 Head: Excelsior 594 (CDRH model) Power supply: Excelsior-PS-XC-CDRH

593.70.5 nm (30, 50 mW)

CW red diode laser Manufacturer: Melles Griot *1 Head: 56 RCS series (56 RCS/S2782) Power supply: 56 IMA series (56 IMA020)

638-3/+2 nm (20 mW)


640+2/-5 nm (40 mW)


33

*1: To mount this product on the C-LU3EX 3 laser unit, an adapter is necessary. *2: To use this product, an adapter is necessary. *3: This product cannot be mounted on the C-LU3EX 3 laser unit. *4: To mount this product on the L3 position of the C-LU3EX 3 laser unit, the C-LU3EX594 DM set (MHV55100)

is necessary. *5: Contact your nearest Nikon representative when mounting this product.

For the beam divergence and the maximum power, see the manual supplied with each laser. Table 2.1-3: Recommended filters

1st wheel 2nd wheel 3rd wheel Excitation wavelength 1st DM

ch1 ch2 ch3 ch4

405/488 ---

405/488/561 ---

405/488/561/638

A1-F 450/50 (MHE57010)

position 1

A1-F 525/50 (MHE57030)

position 1 A1-F 595/50 (MHE57040)

position 1 A1-F 700/75 (MHE57040)

position 1

405/488/543 ---

405/488/543/638

A1-F 450/50 (MHE57010)

position 1

A1-F 515/30 (MHE57060)

position 3

A1-F 585/65 (MHE57070)

position 2 A1-F 700/75 (MHE57070)

position 2

457/514 A1-F 482/35 (MHE57020)

position 2

A1-F 540/30 (MHE57050)

position 2 --- ---

457/514/561 A1-F 482/35 (MHE57020)

position 2

A1-F 540/30 (MHE57050)

position 2

A1-F 595/50 (MHE57040)

position 1 ---

440/514/594 * A1-F 482/35 (MHE57020)

position 2

A1-F 550/49 594LP(MXB22376)

position 4 --- ---

* Transmitted light of 594 LP is received at ch4. Only manual mode is allowed for filter setting.

34

Part Descriptions3

3.1 Scan Head

The scan head is the main optical unit of the system. It is made up of various components, including the galvano-scanner, high-speed scanner (only in A1R), pinhole unit, 1st dichroic mirror, and optical system components.

The excitation light from the laser unit is led into the laser port 1 on the scan head via the two fiber-optic cables for excitation light. The fluorescence from the specimen is led into the connected detector units via fiber-optic cables.

The selection of excitation light and detector unit is automatically made by the command from the PC.

With the A1-SHR A1R scan head & controller, an observation using the high-speed scanner with 405-nm laser stimulation and laser excitation with 488 nm or longer laser can be performed. With the A1-SHRB A1R scan head & controller, an observation using the high-speed scanner with 488-nm laser stimulation and 488-nm laser excitation can be performed.

WARNING

Do not remove the scan head from the microscope. Removing the scan head will result in unintended exposure to hazardous laser light emitted through the scan head mount.

When the laser is being fired, a powerful laser light is emitted from the external port. Do not peer through the external port.

Figure 3.1-1

A B

View of arrow A View of arrow B

(3)

(2)

(1)

(6)

(4)

(5)

(8)(7)

3 Part Descriptions >> 3.1 Scan Head

35

(1) Laser port 1 This port is used for letting the excitation light (laser beam) from the 4 laser unit A into the scan head. Connect the standard fiber-optic cable (single-mode) for excitation light.

(2) Laser port 2 This port is used for letting the excitation light (laser beam) from the 3 laser unit EX into the scan head. Connect the optional fiber-optic cable (single-mode) for excitation light.

(3) Standard detection port This port is used for transmitting the fluorescence coming through the pinhole to the 4 detector unit or GaAsP multi detector unit equipped with filters. Connect the fiber-optic cable (high-efficiency, multi-mode) supplied with the 4 detector unit or GaAsP multi detector unit.

(4) External port for optional use This port is used to transmit the fluorescence coming through the pinhole to the optional external detector unit. You can use an FC connector fiber with a flat end face to connect the port and the detector unit. To mate thefiber, remove the screw which secures the flap cover of the port, open the cover and then remove the cap from the FC connector inside the port.

(5) Spectral detection port This port is used for transmitting the fluorescence coming through the pinhole to the spectral detector unit with a diffraction grating. Connect the fiber-optic cable (high-efficiency, multi-mode) for the spectral detector unit.

(6) Connectors The following connectors are used to connect the control cables to the scan head:

Rectangular-shaped connector: for control of the scan head motor GX connector: for galvano pair signal for X-axis galvano scanner control GY connector: for galvano pair signal for Y-axis galvano scanner control HX connector: for high-speed pair signal for X-axis resonant scanner control (equipped only on the A1R model) HY connector: for high-speed pair signal for Y-axis galvano scanner control (equipped only on the A1R model)

(7) Laser indicator The green indicator lamp lights up when the laser is ready to emit beam through the objective and the external port. The laser does not emit beam when the indicator lamp is not lit.

(8) VAAS detection port This port is used for transmitting the fluorescence coming through the In-focus pinhole and Near-focus pinhole to the 4 detector unit. Connect the fiber-optic cable for VAAS detection.

Table 3.1-1: 1st DM positions

Position Selected wavelength Position Selected wavelength

1 405/488 6 BS 20/80

2 405/488/561 405/488/543

3 405/488/561/638 457

4 457/514 457/514/561

5 405/488/543/638

7, 8 (optional)

440/514/594

3 Part Descriptions >> 3.2 Controller

36

3.2 Controller

The controller is used to control this system and has been adjusted with a set of the scan head.

The controller controls each unit and modulated laser, and generates images. See Chapter 13, Cable Connections between Units for information on wiring.

CAUTION

Always use power cords that satisfy the appropriate ratings. Make sure your power cords meetthe local safety requirements.



Figure 3.2-1

Figure 3.2-2

(9)

(2)(2)

(3)

A

(1)

Standard configuration (5)

(6)

(9) (7) (10) (8) (10) (4) (4)

(11)

View of arrow A

3 Part Descriptions >> 3.2 Controller

37

(1) Indicator Three-color (red, green and orange) LED to display an operating state of the controller.

(2) Cooling fan (3) Connectors

GX connector: for galvano pair signal for X-axis galvano scanner control GY connector: for galvano pair signal for Y-axis galvano scanner control HX connector: for high-speed pair signal for X-axis resonant scanner control (equipped only on the A1R model) HY connector: for high-speed pair signal for Y-axis galvano scanner control (equipped only on the A1R model)

(4) Air intake (5) Main power switch with breaker

This is the main power switch of this unit. When this switch is pressed, power is supplied to the primary source only, rendering the secondary source in a stand-by state.

(6) AC inlet (7) Front-side cable duct (8) Rear-side cable duct (9) Front-side wheels with adjusters (10) Rear-side wheels (11) Remote power switch

This is the remote power switch of this unit. When this switch is pressed, power is supplied to the secondarysource and the controller unit starts up.

3 Part Descriptions >> 3.3 Laser Units

38

3.3 Laser Units

The laser unit is designed to lead the laser beam into the scan head through a single-mode optical fiber. The following units are available. Use one of them with a single-mode fiber-optic cable.

CAUTION

The laser unit is designed to be used on top of the LU-LR 4 laser PS rack. Refer to Section 18.7.1, System Configurations. The laser unit is so heavy that it can cause injury when dropped. To avoid injury, be careful not to accidentally push the laser unit off the LU-LR 4 laser PS rack.

3.3.1 C-LU3EX 3 Laser Unit (Optional)

This laser unit is attached with the violet diode lasers (405 nm and 440 nm), the air-cooled argon ion laser (457 to 514 nm), the blue solid-state laser (488 nm), the green helium-neon laser (543.5 nm), the yellow solid-state laser (561 nm), the orange solid-state laser (594 nm) and the red diode laser (638 nm only) listed in Table 2.1-2, Recommended lasers in Chapter 2, System. To adjust laser intensity, attach the AOM and control it with software. Each laser is equipped with a software-controlled motorized shutter. For multiple-line Ar laser, use of the optional C1-AREX EX changer enables selection of 488-nm wavelength output. In case this laser unit is the only laser unit in this system, only manual mode is allowed for filter setting. When using this laser unit in combination with the LU4A 4 laser unit A to use 561-nm and 543.5-nm lasers, mount the 561-nm laser on the LU4A 4 laser unit A and the 543.5-nm laser on this laser unit.

Figure 3.3-1 C-LU3EX 3 laser unit

Figure 3.3-2 AOM controller

(3) (4)

(1)

(2)

(7)

(8)

(5)

(12)

(11)

(10)

(9)

(6)


39

(1) Fiber-optic cable inlet (single-mode) This inlet provides a connection for the single-mode fiber-optic cable.

(2) C1-AREX EX changer This is an optional switch unit for selecting the air-cooled argon ion laser. Use this unit to select Ar laser wavelength (488 nm only).

(3) AOM connector This connector is used for driving the AOM.

(4) Shutter connector This connector is used to control the shutters inside this unit.

(5) POWER switch This is the main power switch of the AOM controller. When this switch is on, power is supplied to the primary source only. Power supply to the secondary sourceis subject to the state of the REMOTE switch (6).

(6) REMOTE switch This is the remote power switch of the AOM controller. When this switch is on, power is supplied to the secondary source in conjunction with the power supply to theA1 controller. When this switch is off, power is supplied to the secondary source when the POWER switch (5) is on, independently of the power supply to the A1 controller.

(7) AC inlet (8) C1-SYNC connector

This connector is used to input sync signal. (Not for use in this system)

(9) PC connector This connector is used to control the AOM.

(10) LD-MOD connector (not used) (Not for use in this system)

(11) LD-PWR connector This connector is used for the LD power control.

(12) AOM connector This connector is used for driving the AOM.


40

3.3.2 LU4A 4 Laser Unit A

This laser unit is mounted with four kinds of lasers (with one laser product per color) selected from those listed in Table 2.1-2, Recommended lasers in Chapter 2, System. Contact your nearest Nikon representative when mounting the green solid-state laser (514 mm or 532 mm). To adjust laser intensity, control the AOTF with software. Each laser on this unit is equipped with a software-controlled motorized shutter. Each one of the four orange LEDs on the front of the unit turns on when the motorized shutter is opened and the corresponding laser is emitted into the scan head. When the motorized shutter is closed, the corresponding LED is turned off. Laser light is emitted only when capturing images. A return mask and both-end masks are used to shield laser light.

Figure 3.3-3

Figure 3.3-4 (1) POWER switch

This is the main power switch of this unit. When this switch is on, power is supplied to the primary source only. Power supply to the secondary sourceis subject to the state of the REMOTE switch (3).

(2) AC inlet (3) REMOTE switch

This is the remote power switch of this unit. When this switch is on, power is supplied to the secondary source in conjunction with the power supply to theA1 controller. When this switch is off, power is supplied to the secondary source when the POWER switch (1) is on, independently of the power supply to the A1 controller.

(4) CONTROLLER connector This connector is used to control the shutters inside this unit.

(5) AOTF/PC connector This connector is used to control the AOTF.

(6) Interlock cable connectors These connectors are for the cables to connect this unit to the controller and the microscope. The TIRF connector is for the shorting pin jack supplied with the LU4-A1 connection kit A.

(7) LUSU connector This connector is for future expansion. In this system, however, the rectangular shorting connector supplied with the LU4-A1 connection kit A is connected to this connector.

(8) USB connector This connector is for future expansion. (Not for use in this system)

(9) LD connector This connector is for future expansion. (Not for use in this system. A malfunction occurs if connected.)

Precautions when handling the laser unit The laser head and the fiber-optic cable are precisely aligned in these units.

Avoid subjecting the laser head or fiber-optic cable to vibration or shock. Contact your nearest Nikon representative if you need to remove the laser head from the unit due to laser malfunction or other reasons.

Indicating shutter conditions

(1)

(3)

(9) (6)

(5) (4) (8)

(2)

(7)


41

3.3.3 LU-LR 4 Laser PS Rack 100V/120V/230V (Optional)

The EMISSION key switch (3) on the front panel turns on/off the laser power. The power switch (4) on the rear panel supplies the power to the system. The laser is emitted when the power switch (4) on the rear panel and the EMISSION key switch (3) on the front panel are both set on. In the normal usage condition, the power switch (4) and the 15A breaker (7), and the 5A breaker (8) are set on.

Figure 3.3-5 (1) EMISSION la

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Nikon A1R service manual