The genus Aplastodiscus Lutz, 1950 occurs from central and southeastern Brazil to adjacent Argentina (Frost, 2011). Currently, the genus is composed of 15 species, which are divided in three groups: A. albofrenatus, A. perviridis, and A. albosignatus group with six, two and seven species respectively (Faivovich et al., 2005; Frost, 2011). The latter includes Aplastodiscus eugenioi (Carvalho-e-Silva and Carvalho-e-Silva, 2005), an endemic species from the Atlantic rainforest domain, highly associated with epiphytic bromeliads.

This species has been recorded from the state of São Paulo, in the municipalities of Ubatuba and Caraguatatuba (Hartmann, Hartmann and Haddad, 2004; Carvalho-e-Silva and Carvalho-e-Silva, 2005) and to the state of Rio de Janeiro, in the municipalities of Mangaratiba (type locality), Angra dos Reis, Duque de Caxias, Engenheiro Paulo de Frontin, Magé, Nova Iguaçu and Parati (Carvalho-e-Silva and Carvalho-e-Silva, 2005; Carvalho-e-Silva, Silva and Carvalho-e-Silva, 2008; Salles, Weber and Silva-Soares, 2009). According to Carvalho-e-Silva (2006) and Stuart et al. (2008), the conservation status of A. eugenioi is Near Threatened. The species has been signed to this category since its former geographical distribution was less than ten localities, in an area smaller than 5,000 km2. Also the species is likely to be suffering a continuing decline in extent and quality of its habitat (Carvalho-e-Silva, 2006). However the author suggests that further surveys were very likely to reveal A. eugenioi occurring in other locations.

In this study we i) present new records of Aplastodiscus eugenioi in the state of Rio de Janeiro, ii) provide an updated distribution map for this species and iii) comment on the exact location of a paratype specimen.

During a field expedition in October 2010 at Reserva

Ecológica de Guapiaçu (REGUA), municipality of Cachoeiras de Macacu, state of Rio de Janeiro, southeastern Brazil (22º25’S, 42º44’W, 140 m above sea level), we found an adult male of A. eugenioi (Figure 1; MNRJ 72577), which was calling from an epiphyte bromeliad fixed at a fallen tree. At least six other males were calling around the area. Although this species uses bromeliads as calling site, eggs masses are deposited in canopied forest streams (Hartmann, Hartmann and Haddad, 2004). Aplastodiscus eugenioi is therefore classified as habitual bromelicolous species (sensu Peixoto, 1985) for using bromeliad as shelter or calling site as previously suggested (Carvalho-e-Silva, Carvalho-e-Silva and Silva, 2008).

The specimen of Aplastodiscus eugenioi was collected and housed at the Coleção de Anfíbios do Museu Nacional, Universidade Federal do Rio de Janeiro (MNRJ). Moreover, we examined all the other specimens of A. eugenioi that are housed at the MNRJ (Appendix 1). In its description, two paratypes (ZUFRJ 6932-3) are cited to Raiz da Serra, municipality of Duque de Caxias (Carvalho-e-Silva and Carvalho-e-Silva, 2005). However, a correction should be made regarding the locality of this specimen because Raiz da Serra is actually located in the municipality of Magé.

Herpetology Notes, volume 5: 431-433 (2012) (published online on 11 September 2012)

New records and geographic distribution of Aplastodiscus eugenioi (Anura: Hylidae) in southeastern Brazil

Rodrigo de Oliveira Lula Salles*, Rafael Cunha Pontes, Thiago Silva-Soares

Museu Nacional, Departamento de Vertebrados, Universidade Federal do Rio de Janeiro. Quinta da Boa Vista s/n. CEP 20940-040. Rio de Janeiro, RJ, Brazil

*Corresponding author: sallesbio@gmail.com

Figure 2. Aplastodiscus eugenioi (MNRJ 72577) from Reserva Ecológica de Guapiaçu, municipality of Cachoeira de Macacu, state of Rio de Janeiro. Photograph by T. Silva-Soares.

Rodrigo de Oliveira Lula Salles et al.432

Our study adds two new records to the former geographical distribution of A. eugenioi; one at REGUA, municipality of Cachoeiras de Macacu (collected specimen) and another to the municipality of Guapimirim (22º 31’S , 42º 59’W) (specimens found housed at the MNRJ). The record at REGUA (Figure 2; red star) extends the known geographical distribution of A. eugenioi ca. 50 km east from the nearest record in Raiz da Serra (Figure 2; blue circle 1), becoming the easternmost record for this treefrog. Now, its geographical range goes from the northern coast of Serra do Mar region, state of São Paulo, to the state of Rio de Janeiro at the south-central region, entering the southern portion of Serra dos Órgãos (regional name for that part of the Serra do Mar).

The occurrence area of Aplastodiscus eugenioi reaches now ca. 5590 km2 and, also, it is known to occur in three protected reserves: Reserva Ecológica de Rio das Pedras (Carvalho-e-Silva, Silva and Carvalho-e-Silva, 2008), Parque Natural Municipal da Taquara (Salles, Weber and Silva-Soares, 2009), and Reserva Ecológica de Guapiaçu (presente work). Although these new information could move this species to a lower threatened category, it might be more appropriate to maintain the current conservation status due to the undergoing deforestation and disturbance of its habitat. However, for the reasons outlined above, we recommend that the conservation status of A. eugenioi should be reassessed in IUCN Red List in a short future. We still look forward to find the species occurring in several other localities in

Figure 2. Distribution map of Aplastodiscus eugenioi. Red star: Reserva Ecológica de Guapiaçu, municipality of Cachoeiras de Macacu, Rio de Janeiro (collected specimens). Red circle (collection record): municipality of Guapimirim, Rio de Janeiro. Blue circle (literature records): 1 – Raiz da Serra, municipality of Magé, Rio de Janeiro; 2 – Parque Natural Municipal da Taquara, municipality of Duque de Caxias, Rio de Janeiro; 3 – Tinguá, municipality of Nova Iguaçu, Rio de Janeiro; 4 – Sacra da Família Tinguá, municipality of Engenheiro Paulo de Frontin, Rio de Janeiro; 5 – Serra do Piloto, municipality of Mangaratiba, Rio de Janeiro (Type locality); 6 – Reserva Ecológica Rio das Pedras, municipality of Mangaratiba, Rio de Janeiro; 7 – Ilha Grande, municipality of Angra dos Reis, Rio de Janeiro; 8 – municipality of Parati, Rio de Janeiro; 9 – Picinguaba, municipality of Ubatuba, São Paulo; 10 – municipality of Caraguatatuba, São Paulo.

New records and geographic distribution map of Aplastodiscus eugenioi 433

the southeastern of Brazil expanding and/or filling the gaps of its distribution.

Acknowledgements. We are especially grateful to Nicholas Locke and Raquel Rissopatron de Locke who invited us to carry out biological investigations within the Reserva Ecológica de Guapiaçu, a protected reserve of its own implementation and responsibility. We are thankful to Jéssica Fratani, Nathalia Gonzaga, Raphael Jonas, Rômulo Rodrigues and Thamires Benício for all assistance during fieldwork. To Luiz N. Weber who kindly revised the manuscript. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) provided financial support.

References

Carvalho-e-Silva, A.M.P.T., Carvalho-e-Silva, S.P. (2005): New species of the Hyla albofrenata group, from the states of Rio de Janeiro and São Paulo, Brazil (Anura, Hylidae). Journal of Herpetology 39: 73-81.

Carvalho-e-Silva, S.P. (2006): Aplastodiscus eugenioi. In: IUCN 2011. IUCN Red List of Threatened Species. Version 2011.2. <www.iucnredlist.org>. Downloaded on 24 April 2012.

Carvalho-e-Silva, A.M.T., Silva, G.R., Carvalho-e-Silva, S.P. (2008): Anuros da Reserva Rio das Pedras, Mangaratiba, RJ, Brasil. Biota Neotropica 8: 199-209.

Faivovich, J., Haddad, C.F.B., Garcia, P.C.A., Frost, D.R., Camp-bell, J.A., Wheeler, W.C. (2005): Systematic review of the frog family Hylidae, with special reference to Hylinae: a phyloge-netic analysis and taxonomic revision. Bulletin of the Ameri-can Museum of Natural History 294: 1-240.

Frost, D.R. (2011): Amphibian Species of the World. Version 5.5. (31 January, 2011). Electronic Database accessible at: http://re-search.amnh.org/herpetology/amphibia/ American Museum of Natural History, New York, USA.

Hartmann, M.T., Hartmann, P.A., Haddad, C.F.B. (2004): Visual signaling and reproductive biology in a nocturnal treefrog, ge-nus Hyla (Anura: Hylidae). Amphibia-Reptilia 25: 395-406.

Peixoto, O.L. (1995): Associação de anuros a bromeliáceas na Mata Atlântica. Revista da Universidade Rural, Série Ciência da Vida 17 (2): 75-83.

Salles, R.O.L., Weber, L.N., Silva-Soares, T. (2009): Amphibia, Anura, Parque Natural Municipal da Taquara, municipality of Duque de Caxias, state of Rio de Janeiro, southeastern Brazil. Check List 5(4): 840-854.

Stuart, S.N., Hoffmann, M., Chanson, J.S., Cox, N.A., Berridge, R.J., Ramani, P., Young, B.E. (2008). Threatened Amphibians of the World. Barcelona, Spain, Lynx Edicions; Gland, Swit-zerland, IUCN; and Arlington, Virginia, USA, Conservation International.

Appendix 1. Material examined

BRAZIL: RIO DE JANEIRO: Municipality of Angra dos Reis: Ilha Grande, RJ (MNRJ 48502–48504, 62324–62325, 63936–63937). Municipality of Cachoeiras de Macacu: Reserva Ecológica de Gua-piaçu (MNRJ 45783, 57950, 60197-60198, 72577). Municipality of Duque de Caxias: Parque Natural Municipal da Taquara (MNRJ 47486); Sítio 13, road Cachoeira das Dores, Taquara (MNRJ 54586–54587). Municipality of Guapimirim: (MNRJ 59583–59584); Es-tação Ecológica do Paraíso (MNRJ 45786–45789). Municipality of Parati: Martins de Sá (MNRJ 63926–63927). Municipality of Paulo de Frontin: Morro Azul (MNRJ 21658–21668); Sítio Pau Ferro (MNRJ 66237). Municipality of Silva Jardim: border with Cach-oeiras de Macacu, Serra dos Gaviões (MNRJ 53753-53754). SÃO PAULO: Municipality of Ubatuba: Picinguaba (MNRJ 48414).

Accepted by Zoltan T. Nagy; Managing Editor: Sandra Flechas