National report

Republic of Serbia

presented by Tatjana Labus

Regional Workshop 2: Improving Capacity for Diagnosis of Fish and Molluscan Diseases

Banja Luka, Bosnia and Herzegovina, 20-24 October 2013, Hotel Palas

Water Water resources of resources of

SerbiaSerbia

Water Water resources of resources of

SerbiaSerbia

DunavDunav (Danube) (Danube)

SavaSava

TisaTisa

Morava (Velika, Zapadna, JMorava (Velika, Zapadna, Južna)užna)

DrinaDrina

KolubaraKolubara

TimokTimok

Channel system DTDChannel system DTD

Aquaculture in Serbia

Production in Serbia, tonnesProduction in Serbia, tonnesCommon name Scientific name 2010 2011 2012

Carp Cyprinus carpio 4309.311 5517.826 5760.469

Catfish Silurus glanis 16.579 13.621 19.973

Silver carp Hypophthalmichthys molitrix 307.201 846.418 801.542

Grass carp Ctenopharyngodon idella 147.203 207.860 268.136

Pike Esox lucius 1.733 1.013 4.916

Zander Stizostedion lucioperca 8.431 13.073 8.813

Other 162.466 126.687 510.362

Trout Salmo gairdneri 607.795 641.947 1051.966

TOTAL 5560.719 7368.445 8426.177

Estimated catch in rivers and lakes – near Estimated catch in rivers and lakes – near 66000 t000 t

Aquaculture in Serbia

Production of fish and fish fry for human consumption in ponds

2009 2010 2011 2012

number of workers in the ponds No 878 934 798 751

Aquaculture in SerbiaCarp ponds

measure unit 2009 2010 2011 2012

Area in use ha 8524 8940 8517 8704

Production of fry-total t 4966 4463 3411 4883

Production of fish for human consumption t 7549 8331 7817 7462

Catched T 6560 7322 6833 6853

thereof:

Шаран/carp - /total T 6366 7016 6603 6082

Catched t 5428 6156 6071 5959

Сом/catfish - total t 37 45 22 31

Catched t 32 34 21 29

толстолобик /silver carp- total T 837 747 373 546

Catched T 817 724 357 534

амур бели/grass carp total T 209 403 253 193

Catched t 142 306 219 183

Aquaculture in Serbiatrout ponds

Measure unit 2009 2010 2011 2012

Area in use m2 35792 36791 33255 36302

Production of fry - total t 155 155 201 268Production of fish for human consumption - total t 1068 1066 971 983

Catched t 880 873 796 808

thereof:

калифорнијска пастрмка/rainbow trout - total t 1068 1066 971 983 catched t 880 873 796 808

RIBNJACI U SRBIJIPONDS IN SERBIA

ŠARANSKI RIBNJACICARP PONDS

PASTRMSKI RIBNJACITROUT PONDS

Aquaculture in SerbiaУлов рибе у рекама, језерима и каналима/

Catch fish in the rivers, lakes and canals

Мeasure unit 2009 2010 2011 2012

number of fish guard No 291 244 209 242

fisherman – total No 667 502 493 488

thereof: full time work No 375 318 353 366

number of issued licence for sport and recreative fishing No 106559 84875 78945 80919

Movement/transfersBetween epizootic regions in Serbia –

health certificates; veterinary certificates for import/export

Importation of eggs/fry/live fishImport licence + quarantin in SerbiaVeterinary certificates with consigmentsFrom registered ponds

Aquatic animal diseases

National pathogen listFish diseases 1) Epizootska hematopoetska nekroza - Epizootic

haematopoietic necrosis (EHN)2) Zarazna hematopoetska nekroza - Infectious

haematopoietic necrosis (IHN)3) Oncorhynchus masou virusna bolest-

Oncorhynchus masou virus disease 4) Prolećna viremija šarana - Spring viraemia of

carp (SVC) 5) Virusna hemoragijska septikemija pastrmki - Viral

haemorrhagic septicaemia (VHS)Red- OIE listRed and bold- EU list

Aquatic animal diseases

National pathogen list Other significant diseases of fish 1) Virusna encefalopatija i retinopatija - Viral encephalopathy and

retinopathy 2) Virusna bolest lososa - Viral salmon disease 3) Zarazna anemija lososa - Invectious salmon anaemia (ISA) 4) Zarazna nekroza gušterače - Infectious pancreatic necrosis (IPN) 5) Girodaktiloza - Gyrodactylosis (Infection with Gyrodactylus salaris) 6) Edvardsieloza - Edwardsiella ictaluri 7) Iridoviroza kečige - Red sea bream iridoviral disease

Red- OIE listRed and/or bold- EU list

Aquatic animal diseases

Not on national list but are on OIE list- Koi herpesvirus disease, - Epizootic ulcerative syndromeNot on national list but are on EU list - Bacterial kidney disease (BKD) 

   (Renibacterium salmonidarum) - Furunculosis (Aeromonas salmonicida) - Enteric redmouth disease (ERM) 

   (Yersinia ruckeri)

Diseases recorded in the country

Viral diseasesZarazna nekroza gušterače - Infectious

pancreatic necrosis (IPN)

Bacterial diseasesFurunculosis, Yersiniosis, BKD, MAS, Columnaris…

Parasitic diseasesIchtiophtiriosis, Bothriocephalosis...

Fungal diseasesSaprolegnia

Other health problemsRMS (red mark sindrom)

Diagnostic capacity in the country

Aquatic animal diseases laboratory within country

National reference laboratory for fish diseases- NIVS

Description (diagnostic, research, education)YES -diagnostic, research and education

Scope and species interestCyprinids

Salmonids

Level of diagnostic

Level of diagnostic:Level 1: Gross observation, general necropsy

(external, internal)Level II: Bacteriology, Parasitology, Mycology,

HistologyLevel III: Cell lines – EPC, BF2, CCB, RTG-2,

FHMImmunological tests – SN, ELISA, FAT Molecular techniques – PCR, RT-PCR, qPCR,

LAMP, sequencing

Analysis of diagnostic capacity

List of diseases and diagnostic methods used (samples VHS, furunculosis)

VHS, IHN, SVC, KHV, IPN, EHN – clinical observation, detection of gross changes, histological observation, isolation of virus on cell culture, identification by ELISA, IFAT, RT-PCR, qRT-PCR, gene sequencing

Furunculosis , BKD, – Isolation of bacteria on bacteriological media, identification of genus by selective media and general properties, identification of the species by API or any other kit for biochemical identification, serological identification, PCR, sequencing etc...

Surveillance of nationally listed diseases

Diagnostic methods IPN: Virus isolation – cell culture, ELISA, RT-PCR, qPCR, sequencing VHS: Virus isolation – cell culture, ELISA, RT-PCR, qPCR, sequencing IHN: Virus isolation – cell culture, ELISA, RT-PCR, qPCR, sequencing SVCV: Virus isolation – cell culture, ELISA, RT-PCR, qPCR,

sequencing KHV: Virus isolation – cell culture, PCR, qPCR, sequencing BKD: Isolation, FAT, PCR, sequencing

Sampling Active surveillance (restricted trade according to control status of the

farm, trout 2x year clinical + sampling, carp 2x year clinical + sampling)

Results IPN positive

Surveillance and monitoring of other pathogens

Targeted pathogens and aquaculture species

(IHN, VHS, KHV + other (national relevance, OIE/EU listed

diseases: IPN, SVC, BKD)

Conclusions and perspectives

Diagnostics are harmonized with EU legislation

(good education of laboratory people, good testing methods,lab and methods are acredited)

We do not have diseases from the OIE list

Future needs

LegislationCategorisation according 2006/88More education of all people in the chain

More money for education, diagnostics...

THANK YOU FOR YOUR ATTENTION