NanoCellect® WOLF® Cell Sorter Cell Sorting to go
WOLF® Technology
This sterile microfluidics cartridge provides an efficient and
gentle way of cell sorting using 5 parameters:
• forward scattering • back scattering and • 3 colours.
It allows 2-way bulk cell sorting in 1.5 or 5 mL tubes.
The WOLF® technology utilizes a low voltage Piezoelectric actuator.
This feature reduces shear stress and eliminates aerosols.
Flipbook
Happy cells with the WOLF® Cell Sorter
Whether you are focused on biopharma, genomics, or basic research,
NanoCellect® delivers sterile, portable and intuitive solutions for
gentle cell sorting.
The WOLF® Cell Sorter and N1 Single Cell Dispenser are de- signed
to empower biomedical scientists and accelerate re- search. With
its compact size, you can bring the convenience of cell sorting to
YOUR benchtop.
> The WOLF® enables faster workflow and better results.
V E N TSH EA TH
SA MP LE
A B C
High viability: The industry leader for gentle cell sorting at
<2 psi
Small Footprint: fits in a hood easily
100% sterile & disposable fluidics path and cartridge.
Simple and intuitive software. Ready to use after 1 day of
training.
Safe. Reduced aerosols, no sample carryover.
Air Damper
Piezo actuator
Applications
Gene Editing
The WOLF® recovers more clones and is providing you with an
enhanced percentage of a normal karyotype.
Fewer, clear peaks Good quality total RNA
Partially degraded total RNA Many Peaks, degraded total RNA
Low HighPressure and shock
Basic Research
The WOLF® has the highest post-sort viability, especially with
sensitive cells
Quality of nucleic acid samples
Outgrowth of single CHO GFP+ cellsSingle cell cloning and
karyotypes
Competitor: 3 clones, 0% Normal WOLF®: 90 clones, 75% Normal
CHO GFP+ cells
Cell Line Development
Gentle cell sorting and high density monoclonal plating results in
impro- ved cell line development.
100
Postsort viability after 4 hours
K562 cells. PI for dead and Calcein AM for live normalized to
unsorted controls. iPSCs
Postsort viability after 24 hours Colonies <1 week Colonies
>1 week
100%
95%
90%
85%
80%
20
15
10
5
Genomics
High Pressure and Shock Decompres- sion can affect RIN score for
Genomic Sample Analysis
Viability of cells
N1 Cell Dispenser
The optional N1 Cell Dispenser with the possibility to sort into
various plates is a great extra and makes the WOLF® comple- te.
Single cell sorting is now easy and reliable.
100
0
80
60
40
90.60%
Single cell dispensing accuracy
Germany | Netherlands | France | United Kingdom | Nordic | Ireland
| Sweden
I&L Biosystems GmbH Königswinterer Straße 409 53639
Königswinter • Germany Tel.: +49 2223 9192-0 Fax: +49 2223 9192-48
[email protected] www.il-biosystems.com
OPTICS Excitation 488 nm; 30 mW diode laser
(rated at 45.000 h life time) Laser profile 25 x 75 µm Scatter
detection Forward: 0°, +/-15
Back: 180°, +/-15 Emission detection 3 colors,
525 ± 25 nm (FITC, Calcein, GFP, Alexa 488) 585 ± 20 nm (PE, PI;
Alexa 568) 665 nm Longpass (PE-Cy5/-Cy7, PerCP/Cy5.5, 7AAD)
Optical alignment Fixed alignment, no maintenance required
PERFORMANCE Scatter resolution resolves lympho-, mono- and
granulocytes Fluorescence resolution 8 peak separation with the
Spherotech Rainbow calibration beads
in the 3 fluorescence channels Analysis speed > 5000/s Sorting 1
and 2 way Back-to-back Sorting speed 300 events/s Absolute counts
yes Volumetric Analysis yes Warm-up time less than 1 minute Sorting
Output 1.5 and 5 mL, 96- and 384-well plates Sorting purity up to
99%
FLUIDICS Sample Input: 1.5 and 5 mL tubes
Flow rate: 24 µL/min Minimal volume: 150 µL
Sheath (PBS or buffer of choice)
Input: 50 mL conical tubes Flow rate: 160 µL/min Usage: 7.5
mL/h
Dead volume 50 µL Tubing diameter (inner)
200 – 500 µm
50 µm
