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Multiscale model of scaffold for heart valve for in situtissue engineeringArgento, G.; Oomens, C.W.J.; Baaijens, F.P.T.
Published: 01/01/2010
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Citation for published version (APA):Argento, G., Oomens, C. W. J., & Baaijens, F. P. T. (2010). Multiscale model of scaffold for heart valve for in situtissue engineering. Poster session presented at conference; Mate Poster Award 2010 : 15th Annual PosterContest, .
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Download date: 11. Nov. 2018
Multiscale model of scaffold for heart
valve for in situ tissue engineering
G. Argento, C.W.J. Oomens, F.P.T. Baaijens
Eindhoven University of Technology, Department of Biomedical Engineering
/ Soft tissue Biomechanics & Engineering
IntroductionThe traditional tissue engineering approach requires the
growth of tissue on a scaffold in a bioreactor before
implantation (1). An attractive alternative is in situ tissue
engineering by designing an instructive electrospun
scaffold, able to meet the hemodynamic demand when it is
implanted, being sufficiently strong and durable, and able
to promote cellular ingrowth, proliferation and
differentiation, and in-vivo tissue maturation.
Aim of the projectThe aim of the present work is to develop a computational
model of electrospun scaffolds based on microstructural
parameters (fiber diameter, anisotropy, fibers bonding, void
volume fraction). The model aims at being useful to
optimize the micro- and macroscopic properties of the
electrospun scaffolds.
Materials and methodsThe Driessen’s constitutive model (2) describes the
angular fibers distribution in cardiovascular tissue through
a periodic version of the normal probability distribution
where α indicates the main fibers angle and β indicates the
dispersity of fibers distribution.
A representative volume element (RVE) of electrospun
scaffold is built by processing the model through an
algorithm that generates a distance-based fiber dispersion
based on microstructural parameters (Fig 1).
Figure 1: Process of modeling of the scaffold fibrous network
cos 2 1expi iA
f
A continuum layer with relatively low stiffness overlayed to
the discrete network represents the neo-tissue that is
formed in-situ.
Ties between the discrete and the continuum layer mimic
the in vivo status (Fig 2). Periodic boundary conditions
describing a biaxial deformation are applied to the
continuum layer with the aim of performing the complete
mechanical characterization of the network (Fig 3).
Figure 2: Model of scaffold RVE with description of microstructural parameters
ResultsThe described model represents a tool for designing a
structural model of a scaffold for in situ tissue engineering.
The output of a multi-scale analysis of the in-vivo behavior
of the scaffold is suitable for addressing the process of
spinning of an electrospun scaffold .
Figure 3: Scaffold RVE in the deformed configuration
Future workThe coupling of the micro- and macromechanical behavior
of the scaffold will be performed to fit the micromodel to the
macromechanical behavior of the natural heart valve.
Fiber diameter
Porosity
OrientationFiber bonding
References
(1) Mol A, Driessen NJB, Rutten MCM, Hoerstrup SP, Bouten CVC, Baaijens FTP.
Tissue Engineering of Human Heart Valve Leaflets: A Novel Bioreactor for a
Strain-Based Conditioning Approach. Ann Biomed Eng 2005; 12(33):1778-1788.
(2) Driessen N, Modeling and remodeling of the collagen architecture in
cardiovascular tissue, Thesis.