MicroFill Operator's Manual - Pera Medikalperamed.com/peramed/docs/microfill operator manual.pdf · vi | Preface BioTek Instruments, Inc. Decontamination Procedure ..... 59 Daily/Overnight

Operator’s Manual

Microplate Dispenser

MicroFill™

.

MicroFill™

Microplate Dispenser, All Models Operator’s Manual

January 2013 © 2013 Part Number 7141000 Revision G BioTek® Instruments, Inc.

ii | Preface

BioTek Instruments, Inc.

Notices

BioTek® Instruments, Inc. Highland Park, P.O. Box 998 Winooski, Vermont 05404-0998 USA

All Rights Reserved

© 2013, BioTek® Instruments, Inc. All rights are reserved. No part of this manual may be reproduced or transmitted in any form, or by any means electronic or mechanical, including photocopying and recording for any purpose other than the purchaser’s personal use without written permission of BioTek® Instruments, Inc.

Trademarks

BioTek is a registered trademark, and MicroFill and BioStack are trademarks of BioTek Instruments, Inc. All other trademarks are the property of their respective holders.

Restrictions and Liabilities

Information in this document is subject to change and does not represent a commitment by BioTek. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.

Contents | iii

MicroFill Operator's Manual

Contents

Notices ................................................................................. ii Contact Information ............................................................... ii Revision History .....................................................................ix Document Conventions ...........................................................xi Intended Use Statement ........................................................ xii Quality Control ...................................................................... xii Warranty and Product Registration........................................... xii Repackaging and Shipping ...................................................... xii Warnings ............................................................................ xiii Hazards .............................................................................. xiii Precautions ......................................................................... xiv CE Mark .............................................................................. xvi Electromagnetic Interference and Susceptibility ....................... xvii User Safety ....................................................................... xviii Safety Symbols .................................................................... xix Chapter 1: Introduction ........................................................ 1

The MicroFill Microplate Dispenser ........................................ 2 Models ......................................................................... 2 Manifolds ..................................................................... 3 Software ...................................................................... 3 Compatibility with the BioStack™ Microplate Stacker .......... 4

Hardware Features ............................................................. 5 Package Contents .............................................................. 6 Optional Accessories .......................................................... 7 Specifications .................................................................... 8

Chapter 2: Installation ....................................................... 11

1: Unpack and Inspect the Instrument ................................ 12 2: Select an Appropriate Location ....................................... 12 3: Install the MicroFill’s Components .................................. 13

Microplate Carrier ........................................................ 13 Microplate Adaptor ...................................................... 13 Priming Plate .............................................................. 13 Manifold ..................................................................... 14 Syringe Head .............................................................. 15 Check Valves, Tubes, and Bottles .................................. 16 Check Valves .............................................................. 16

iv | Preface


Supply Tubes .............................................................. 17 Bottles ....................................................................... 17 Final Check ................................................................ 17 Prime Trough Drain Kit ................................................. 19 Mist Shield ................................................................. 20 Power Supply and Cords ............................................... 21 Data Communication ................................................... 21 Serial Port Communications with Other Devices ............... 21 Operating the MicroFill with the BioStack ........................ 21

4: Verify Performance ...................................................... 22 Repackaging and Shipping Instructions ............................... 23

Before shipping the instrument: .................................... 23 Repackaging the instrument and its accessories: ............. 23

Chapter 3: Operation .......................................................... 25

Turn On/Off the MicroFill ................................................... 26 Keypad Description .......................................................... 26 Operation with the BioStack .............................................. 28 Main Menu ...................................................................... 28 Program Types ................................................................ 29 Prime Programs ............................................................... 29

Priming Considerations ................................................. 29 Define Prime Program .................................................. 31 Define Prime Program Options ...................................... 31 Run Prime Program ..................................................... 31 Edit Prime Program...................................................... 32 Copy Prime Program .................................................... 32 Copy Prime Program Options ........................................ 32 Delete Prime Program .................................................. 32 Delete Prime Program Options ...................................... 32

Dispense Programs .......................................................... 33 Define Dispense Program ............................................. 33

Define Dispense Program Options ............................. 33 Run Dispense Program ................................................. 35

Run Dispense Program Options ................................. 35 Soak Programs ................................................................ 35 Link Programs ................................................................. 36

Define Link Program ............................................................. 36 Define Link Program Options .................................................. 36

Utility Menu Options ......................................................... 37 TESTS ....................................................................... 37

Self-Check ............................................................. 37

Contents | v


Configuration Information (CHKSUM) ......................... 37 SETUP ....................................................................... 37

Language .............................................................. 37 Manifold ................................................................ 37

BIOSTACK .................................................................. 38 AUTOPRIME ................................................................ 38

Editing the AutoPrime Program ................................. 38 AutoPrime Program Options ..................................... 38 AutoRinse .............................................................. 39

Chapter 4: Preventive Maintenance .................................... 41

Overview ........................................................................ 42 Recommended Maintenance Schedule for the MicroFill .......... 43 Tools and Supplies ........................................................... 43 Maintenance and Prime Programs ...................................... 44 Daily Maintenance ............................................................ 45

Rinse and Soak ........................................................... 45 Removing Protein Residuals and Fungi Growth ................. 46

Daily ..................................................................... 46 Weekly/Monthly ...................................................... 47

AUTOPRIME ................................................................ 48 Overnight Maintenance ......................................................... 49 Monthly Maintenance ............................................................ 50

Clean the Bottles and Tubes .............................................. 50 Clean the Manifold ........................................................... 50 Clean (“Stylize”) the Dispense Tubes .................................. 50 Clean the Plate Carrier...................................................... 51 Clean the Plate Carrier Transport Rail ................................. 52 Clean/Replace the Check Valves......................................... 52 Calibrating the Backlash ................................................... 53

Equipment Required .................................................... 53 Setup ........................................................................ 53 Select the Manifold (AF1000A Dispensers Only) ............... 53 Create a Dispense Program ........................................... 54 Prime the Plate ........................................................... 54 Calibrate the Backlash ................................................. 54

Autoclaving the Syringe Head ............................................ 56 Long-Term Storage Maintenance ............................................ 57 Storing the MicroFill Dispenser ............................................... 57 Decontamination .................................................................. 58

Purpose .......................................................................... 58 Tools and Supplies ........................................................... 59

vi | Preface


Decontamination Procedure ............................................... 59 Daily/Overnight Maintenance Log Monthly Maintenance Log

Chapter 5: Instrument Qualification ................................... 61

IQ/OQ/PQ ....................................................................... 62 Recommended Qualification Schedule ................................. 63 System Self-Check and Checksum Test ............................... 64

System Self-Check ...................................................... 64 Checksum Test ........................................................... 64

Dispense Precision and Accuracy Tests ................................ 64 Overview ................................................................... 64 Specifications ............................................................. 65 Precision .................................................................... 65 Accuracy .................................................................... 65 Materials .................................................................... 66 Test Solutions ............................................................. 67

For 16-Channel Manifold Testing: .............................. 67 For “8-96” Manifold Testing: ..................................... 67 For “8-24” Manifold Testing: ..................................... 67

Dispense Precision & Accuracy Test: 16-Channel Manifold . 69 Dispense Precision & Accuracy Test: “8-96” Manifold ........ 70 Dispense Precision & Accuracy Test: “8-24” Manifold ........ 72 Documenting Test Results ............................................ 74 Liquid Test Worksheets Performance Verification Logs

Chapter 6: Troubleshooting ................................................ 75

Overview ........................................................................ 76 General Usage Guidelines ................................................. 77

Before Running Any Program: ....................................... 77 Preventive Maintenance ............................................... 77 Programming considerations ......................................... 77

Product Support & Service ................................................ 78 Returning Instruments for Service/Repair ....................... 78 Contacting BioTek for Applications Support ..................... 78

Troubleshooting Charts ..................................................... 79 Dispenser Start-Up ...................................................... 79 Syringe Movement ...................................................... 79 Fluid Delivery ............................................................. 80 Fluid Leakage ............................................................. 81 Microplate Carrier Movement ........................................ 82

Contents | vii


Dispense Manifold Movement ........................................ 82 Appendix A: Error Codes ..................................................... 83

Overview ........................................................................ 84 General Errors ................................................................. 84 BioStack Errors ............................................................... 87 Fatal Errors ..................................................................... 88

Appendix B: Chemical Compatibility ................................... 89 Autoclavable MicroFill ....................................................... 90

Table 1. Material/Where Used List .................................. 90 Table 2. Chemical Compatibility Ratings ......................... 91

Non-Autoclavable MicroFill ................................................ 92 Table 3. Material/Where Used List .................................. 92 Table 4. Chemical Compatibility Ratings ......................... 93

Appendix C: Dispense Flow Rates ....................................... 95

Standard (Non-Autoclavable) Versions of the MicroFill™ ........ 96 Autoclavable Versions of the MicroFill ................................. 97

16-Channel Manifold .................................................... 97 “8-96” Manifold ........................................................... 97 “8-24” Manifold ........................................................... 97

Appendix D: Packing Instructions ...................................... 99

viii | Preface


Contact Information

See also “Product Support & Service” in Chapter 6.

BioTek® Instruments, Inc. Highland Park, P.O. Box 998 Winooski, Vermont 05404-0998 USA

Customer Service and Sales

Internet: www.biotek.com

Phone: 888-451-5171 (toll free in the U.S.) 802-655-4740 (outside the U.S.)

Fax: 802-655-7941

E-Mail: [email protected]

Service/TAC

Phone: 800-242-4685 (toll free in the U.S.) 802-655-4740 (outside the U.S.)

Fax: 802-654-0638


European Coordination Center/ Authorized European Representative

BioTek® Instruments GmbH Kocherwaldstrasse 34 D-74177 Bad Friedrichshall Germany

Internet: www.biotek.de

Phone: +49 (0) 7136 9680

Fax: +49 (0) 7136 968 111


Revision History | ix


Revision History

Rev Date Changes

A 08/2001 First Issue

B

01/2002

Added new chemical compatibility caution, page v. Inserted new text throughout manual concerning autoclavable version of the instrument. Changed “priming trough” to “priming plate” and “pre-priming trough” to “priming trough.” Updated Package Contents and Optional Accessories list. Revised basecode software to include a “repeat” feature, pages 3-4 and 3-7. Added new Figures 2-2, 2-4, 4-1 and D-1. Revised range of prime and dispense flow rates, Chapters 3 and 4. Added three new appendices C, D, and E.

C 04/2003 Updated contact information and safety symbol text in Preface. Added information concerning the 24-channel manifold optional accessory to: Chapters 1, 3, 4; Appendices A and B; Glossary. Added information regarding the compatibility of µFill Microplate Dispenser models with the BioStack™ Automated Microplate Stacking System to: Chapters 1-5, Appendix A, and the Index. Moved the reference to the serial cable (part no. 75053) from Optional Accessories to Package Contents, page 1-4. In Optional Accessories list, indicated which accessories are only used with the AF1000A model, page 1-4. Clarified callouts in Figure 2-3, page 2-5. Under Prime Volume (page 3-7), changed “The volume range is 5 to 1500 µl/well/sec” to “…5 to 1500 µl/well.” Added a reference to the 8-channel manifold as an option for AF1000A model users, page 4-8. Updated error codes in Appendix A. Added information for blue dye test solution to: Performance Verification Testing section (page 4-16), and Appendix B, Dye Preparation for Performance Testing. Added 8-channel manifold information to: Appendix E, Dispense Flow Rates.

D 10/2003 Updated Intended Use statement.

E 11/2006 General: Applied new company standard template and logo. Incorporated contents of Manual Update revision D1. Preface: Added WEEE directive and symbol. Added pinch point Safety Symbol. Updated product description under Intended Use. Removed Warranty. Ch 1 Introduction: Clarified manifold descriptions (AF1000 and AF1000A models). Added BioTek part numbers under Package Contents. Moved Priming Trough Drain Kit from Optional Accessories to

x | Preface


Rev Date Changes

Package Contents. Ch 2 Installation: Restructured sequence of steps to better reflect actual practice. Added Prime Trough Drain Kit installation instructions (from former Appx D). Ch 3 Operation: Added Utility Menu Options section (moved from original Ch 4). Ch 4 Preventive Maintenance: Removed information on Performance Verification (moved to new Ch 5). Added instructions for Removing Protein Residuals and Fungi Growth. Moved AutoPrime to Daily Maintenance section. Enhanced Decontamination procedure and added step to put priming plate in microplate adaptor. Moved Utility section to Ch 3. Moved Performance Verification to Ch 5. Ch 5 Instrument Qualification. New chapter containing previous Performance Verification information. Enhanced test procedures in line with µFill IQOQPQ procedure. Added Liquid Test worksheets. Ch 6 Troubleshooting. Formerly Ch 5. Minor enhancements to Troubleshooting Charts. Removed original Appendix B, Dye Preparation for Performance Testing. Moved this information to Ch 5 Instrument Qualification. Appx B Chemical Compatibility (formerly Appx C). Updated with latest information available via BioTek’s website. Removed original Appendix D, Priming Trough Drain Kit Assembly. Moved this information to Ch 2. Appx C Dispense Flow Rates (formerly Appx E). Removed redundant/inappropriate section “For 8-Channel Manifolds”.

F 2/2008 Throughout: Changed all instances of “BioStack” to “BioStack” and “µFill” to “MicroFill”. Preface: Updated the Intended Use Statement. Updated Directives information and Safety Symbols.

G 1/2013 Preface: Added Repackaging and Shipping statement. Under “Hazards” added Service, Accessories, Software Quality Control, and Pinch Hazard warnings. Under “Precautions” added Bovine Serum Albumin, Power Supply, Electromagnetic Environment, and Electromagnetic Compatibility precautions. Updated Low Voltage (Safety) Directive and added EN 61010-2-081. Added EN 61010-2-101 to IVD Directive. Updated Safety Symbols. Chapter 2, Installation: Replaced outdated packing instructions with a reference to new Appendix D. Added Appendix D, Packing Instructions.

Document Conventions | xi


Document Conventions

See also Safety Symbols on page xix:

Example Description

This icon calls attention to important safety notes.

Warning! A Warning indicates the potential for bodily harm and tells you how to avoid the problem.

Caution A Caution indicates potential damage to the instrument and tells you how to avoid the problem.

DEFINE Text in COURIER font represents menu options as they appear on the instrument’s display.

Note: Bold text is primarily used for emphasis.

This icon calls attention to important information.

xii | Preface


Intended Use Statement

BioTek’s MicroFill Microplate Dispenser accommodates 96-, 384- and 24-well standard microplates and deep well blocks for bulk reagent dispensing applications. An autoclavable model is available for those applications requiring a sterile fluid path. The MicroFill can operate with standard robotic systems, such as BioTek’s BioStack Microplate Stacker.

The intended use of this instrument is dependent on the instrument’s labeling. If there is an IVD label, then the instrument may be used for clinical, research and development, or other nonclinical purposes. If there is no such label, then the instrument may only be used for research and development, or for other nonclinical purposes.

Quality Control

It is considered good laboratory practice to run laboratory samples according to instructions and specific recommendations included in the assay package insert for the test to be conducted. Failure to conduct Quality Control checks could result in erroneous test data.

Warranty and Product Registration

Take a moment to review the Warranty information that shipped with your product. Please also register your product with BioTek to ensure that you receive important information and updates about the product(s) you have purchased. You can register online through the Customer Resource Center at www.biotek.com or by calling 888/451-5171 or 802/655-4740.

Repackaging and Shipping

If you need to ship the instrument to BioTek for service or repair, contact BioTek for a Return Materials Authorization (RMA) number, and be sure to use the original packing materials. Other forms of commercially available packaging are not recommended and can void the warranty. If the original packing materials have been damaged or lost, contact BioTek for replacement packing.

Warnings | xiii


Warnings

Operate the instrument on a level, stable surface away from excessive humidity.

When operated in a safe environment according to the instructions in this document, there are no known hazards associated with the instrument. However, the operator should be aware of certain situations that could result in serious injury. See Hazards and Precautions.

Strict adherence to instrument maintenance and qualification procedures is required to ensure accurate dispense volumes and risk-free operation.

Hazards

The following hazard warnings are provided to help avoid injury:

Warning! Power Rating. The instrument’s power supply must be connected to a power receptacle that provides voltage and current within the specified rating for the system. Use of an incompatible power receptacle may produce electrical shock and fire hazards.

Warning! Electrical Grounding. Never use a plug adapter to connect primary power to the power supply. Use of an adapter disconnects the utility ground, creating a severe shock hazard. Always connect the system power cord directly to an appropriate receptacle with a functional ground.

Warning! Service. Only qualified technical personnel should perform service procedures on internal components.

Warning! Accessories. Only accessories which meet the manufacturer’s specifications shall be used with the instrument.

Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage into internal components creates a potential for shock hazard or instrument damage. If a spill occurs while a program is running, abort the program and turn the instrument off. Wipe up all spills immediately. Do not operate the instrument if internal components have been exposed to fluid.

Warning! Unspecified Use. Failure to operate the equipment according to the guidelines and safeguards specified in this manual could result in a hazardous condition.

Warning! Software Quality Control. The operator must follow the manufacturer’s assay package insert when modifying software parameters and establishing dispensing methods. Failure to conduct quality control checks could result in erroneous test data.

Warning! Internal Voltage. Always turn off the power switch and unplug the power supply before cleaning the outer surface of the instrument.

xiv | Preface


Warning! Potential Biohazards. Some assays or specimens may pose a biohazard. Adequate safety precautions should be taken as outlined in the assay’s package insert. Always wear safety glasses and appropriate protective equipment, such as chemically resistant rubber gloves and apron.

Warning! Pinch Hazard. Do not reach under the instrument during operation; the syringe pump may pinch your fingers.

Precautions

The following precautions are provided to help avoid damage to the instrument:

Caution: Service. The instrument should be serviced by BioTek authorized service personnel. Only qualified technical personnel should perform troubleshooting and service procedures on internal components.

Caution: Spare Parts. Only approved spare parts should be used for maintenance. The use of unapproved spare parts and accessories may result in a loss of warranty and potentially impair instrument performance or cause damage to the instrument.

Caution: Environmental Conditions. Do not expose the instrument to temperature extremes. For proper operation, ambient temperatures should remain withing the range listed in the Specification section. Performance may be adversely affected if temperatures fluctuate above or below this range. Storage temperature limits are broader.

Caution: Sodium Hypochlorite. Do not expose any part of the instrument to sodium hypochlorite solution (bleach) for more than 20 minutes. Prolonged contact may damage the instrument surfaces. Be certain to rinse and thoroughly wipe all surfaces.

Caution: Buffer Solution. Although many precautions have been taken to ensure that the instrument is as corrosion-proof as possible, the dispenser is not sealed and liquids can seep into sensitive components. Make sure that any spilled buffer solution is wiped off the dispenser. Continuous exposure to salt solution may corrode parts of the microplate carrier, movement rail, springs, and other hardware.

Caution: Chemical Compatibility. Some chemicals may cause irreparable damage to this instrument. The following chemicals have been deemed safe for use in this instrument: buffer solutions (such as PBS), saline, surfactants, deionized water, 70% ethyl, isopropyl, or methyl alcohol, 40% formaldehyde, and 20% sodium hydroxide. Other chemicals may cause severe damage to the instrument. Contact BioTek Instruments if you require further assistance. Refer to Appendix B, Chemical Compatibility.

Precautions | xv


Caution: Bovine Serum Albumin. Solutions containing proteins, such as bovine serum albumin (BSA), will compromise the instrument’s performance over time unless a strict maintenance protocol is adhered to.

Caution: Power Supply. Only use the power supply shipped with the instrument, and operate it within the range of line voltages listed on it.

Caution: Disposal. This instrument contains printed circuit boards and wiring with lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on waste electrical and electronic equipment (WEEE),” or local ordinances.

Caution: Warranty. Failure to follow preventive maintenance protocols may void the warranty. See Chapter 4, Preventive Maintenance.

Caution: Electromagnetic Environment. Per EN 61326-2-6 it is the user’s responsibility to ensure that a compatible electromagnetic environment for this instrument is provided and maintained in order that the device will perform as intended.

Caution: Electromagnetic Compatibility. Do not use this device in close proximity to sources of strong electromagnetic radiation (e.g., unshielded intentional RF sources), because these may interfere with the proper operation.

xvi | Preface


CE Mark

Based on the testing described below and information contained herein, this instrument bears the CE mark.

See the Declaration of Conformity for more specific information.

Directive 2004/108/EC Electromagnetic Compatibility

Emissions - CLASS A EN50081-1 and IEC 61326-1 EN 55022 Class A

This equipment has been designed and tested to CISPR 11 Class A. In a domestic environment it may cause radio interference, in which case, you may need to mitigate the interference.

Immunity EN 50082-1 and IEC 61326-1 IEC 1000-4-2 Electrostatic Discharge IEC 1000-4-3 Radiated EM Fields IEC 1000-4-4 Electrical Fast Transient/Burst IEC 1000-4-5 Surge Immunity EN 61000-4-6 Conducted Disturbances EN 61000-4-11 Voltage Dips, Short Interruptions and Variations

Directive 2006/95/EC Low Voltage (Safety)

The system has been type-tested by an independent testing laboratory and was found to meet the requirements of this Directive. Verification of compliance was conducted to the limits and methods of the following:

EN 61010-1, “Safety requirement for electrical equipment for measurement, control and laboratory use. Part 1, General requirements.”

EN 61010-2-081, “Particular requirements for automatic and semi-automatic laboratory equipment for analysis and other purposes.”

Directive 2002/96/EC Waste Electrical and Electronic Equipment

Disposal Notice: This instrument contains printed circuit boards and wiring with lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on waste electrical and electronic equipment (WEEE)” or local ordinances.

Electromagnetic Interference and Susceptibility | xvii


Directive 98/79/EC In Vitro Diagnostics (if labeled for this use)

Product registration with competent authorities.

EN 61010-2-101, “Particular requirements for in vitro diagnostic (IVD) medical equipment.”

Electromagnetic Interference and Susceptibility

USA FCC CLASS A

RADIO AND TELEVISION INTERFERENCE

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference, in which case the user will be required to correct the interference at their own expense.

In order to maintain compliance with FCC regulations shielded cables must be used with this equipment. Operation with non-approved equipment or unshielded cables is likely to result in interference to radio and television reception.

Canadian Department of Communications Class A

This digital apparatus does not exceed Class A limits for radio emissions from digital apparatus set out in the Radio Interference Regulations of the Canadian Department of Communications.

Le present appareil numerique n'émet pas de bruits radioélectriques depassant les limites applicables aux appareils numérique de la Class A prescrites dans le Réglement sur le brouillage radioélectrique édicté par le ministère des Communications du Canada.

xviii | Preface


User Safety

This device has been type-tested by an independent laboratory and found to meet the requirements of the following:

• Underwriters Laboratories UL 61010-1, “Safety requirements for electrical equipment for measurement, control and laboratory use; Part 1: General requirements.”

• Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety requirements for electrical equipment for measurement, control and laboratory use; Part 1: General requirements.”

• EN 61010 Standards, see CE Mark starting on page xvi.

Safety Symbols | xix


Safety Symbols

Some of these symbols may appear on the instrument or accessories:

Alternating current Courant alternatif Wechselstrom Corriente alterna Corrente alternata

Both direct and alternating current Courant continu et courant alternatif Gleich - und Wechselstrom Corriente continua y corriente alterna Corrente continua e corrente alternata

Direct current Courant continu Gleichstrom Corriente continua Corrente continua

Earth ground terminal Borne de terre Erde (Betriebserde) Borne de tierra Terra (di funzionamento)

On (Supply) Marche (alimentation) Ein (Verbindung mit dem Netz) Conectado Chiuso

Protective conductor terminal Borne de terre de protection Schutzleiteranschluss Borne de tierra de protección Terra di protezione

Off (Supply) Arrêt (alimentation) Aus (Trennung vom Netz) Desconectado Aperto (sconnessione dalla rete di alimentazione)

Caution (refer to accompanying documents) Attention (voir documents d’accompagnement) Achtung siehe Begleitpapiere Atención (vease los documentos incluidos) Attenzione, consultare la doc annessa

Warning, risk of electric shock Attention, risque de choc électrique Gefährliche elektrische schlag Precaución, riesgo de sacudida eléctrica Attenzione, rischio di scossa elettrica

Warning, risk of crushing or pinching Attention, risque d’écrasement et pincement Warnen, Gefahr des Zerquetschens und Klemmen Precaución, riesgo del machacamiento y sejeción Attenzione, rischio di schiacciare ed intrappolarsi

Warning, hot surface Attention, surface chaude Warnen, heiße Oberfläche Precaución, superficie caliente Attenzione, superficie calda

Warning, potential biohazards Attention, risques biologiques potentiels Warnung! Moegliche biologische Giftstoffe Atención, riesgos biológicos Attenzione, rischio biologico

xx | Preface


In vitro diagnostic medical device Dispositif médical de diagnostic in vitro Medizinisches In-Vitro-Diagnostikum Dispositivo médico de diagnóstico in vitro Dispositivo medico diagnostico in vitro

Separate collection for electrical and electronic equipment Les équipements électriques et électroniques font l’objet d’une collecte sélective Getrennte Sammlung von Elektro- und Elektronikgeräten Recogida selectiva de aparatos eléctricos y electrónicos Raccolta separata delle apparecchiature elettriche ed elettroniche

Consult instructions for use Consulter la notice d’emploi Gebrauchsanweisung beachten Consultar las instrucciones de uso Consultare le istruzioni per uso

Chapter 1

Introduction

This chapter introduces the MicroFill™ Microplate Dispenser, and describes its hardware and software features.

The MicroFill Microplate Dispenser ............................................ 2 Models ............................................................................. 2 Manifolds .......................................................................... 3 Software .......................................................................... 3 Compatibility with the BioStack™ Microplate Stacker .............. 4

Hardware Features ................................................................. 5 Package Contents ................................................................... 6 Optional Accessories ............................................................... 7 Specifications ........................................................................ 8

2 | Chapter 1: Introduction


The MicroFill Microplate Dispenser

The MicroFill™ is a self-contained, user-programmable, universal microplate dispenser. The following unique combination of features expands applications far beyond the typical microplate dispenser:

• The microprocessor-controlled syringe pump with its long-lasting seal ensures precise and accurate fluid delivery, as well as reproducibility for repeated dispenses.

• The low-profile, robot-compatible carrier accommodates deep-well microplates and microtubes in 24-, 96-, and 384-well formats. A microplate adaptor accommodates standard height plates.

• User-programmable dispense rates and depths provide for a wide range of dispensing capabilities.

• The horizontal dispense position provides for precise positioning of the wells under the dispense tubes.

• The MicroFill™ can be used to dispense a variety of solutions, including buffered saline and reagent solutions.

• The intuitive menu-driven software allows you to create and store up to 100 dispense programs. When you are ready to run a program, simply select the program name and enter the starting strip and number of strips. The MicroFill will then process the plate according to the program parameters.

• Several predefined programs are provided to simplify preventive maintenance, which should be performed regularly to ensure optimum dispenser performance.

• The MicroFill™ has a low-maintenance design, the result of BioTek’s long history with liquid-handling instruments. The MicroFill™ does not require recalibration.

• In addition to operating on a stand-alone basis, the MicroFill™ is compatible with BioTek’s BioStack™ Microplate Stacker and BioStack Twister II Microplate Handler.

Models

• The AF1000 supports the 16-channel manifold, for standard 96- and 384-well plates. It is not autoclavable and should not be used with organic solvents.

• The AF1000A is autoclavable and can be used with organic solvents. It supports the following manifolds:

16-channel, for standard 96-/384-well plates

8-channel for 96-well standard, half-area, and PCR plates (“8-96”)

8-channel for standard 24-well plates (“8-24”)

The MicroFill Microplate Dispenser | 3


Manifolds

The manifold is the component that delivers fluid to the microwells. Each dispenser is equipped with a manifold containing 16 dispense tubes for dispensing into 96- and 384-well plates. There is one tube per well for 384-well plates and two tubes per well for 96-well plates.

For the autoclavable model (AF1000A), an optional “8-96” manifold is available for dispensing into 96-well half-area and PCR plates. An optional “8-24” manifold is available for dispensing into standard 24-well plates.

See page 8 for information on microplate types and dimensions supported by the different manifolds.

Software

The dispenser is operated by onboard software, controlled through the dispenser’s keypad. This onboard software, referred to as the “basecode,” has an identifying part number.

Your dispenser is installed with basecode part number 7140200. The basecode also has an identifying version number. If you need to contact BioTek Instruments for technical assistance, you will be asked for the part number and version of the basecode currently residing on your dispenser. To locate this information, press these keys from the Main Menu: UTIL > TESTS > CHKSUM.

The basecode part number and accompanying version number will appear for a few seconds, followed by additional configuration information. The display will automatically return to the main menu.

The 7140200 basecode supports the following features:

• An easy to use, menu-driven interface.

• Ability to create Prime, Dispense, and Linked programs for automated dispensing to microplates.

• Storage of up to 100 programs.

• User-definable program names, for rapid recall at program run time.

• Configurable program parameters such as variable dispense flow rates, dispense heights, and starting strip.

• Predefined Maintenance programs, including AutoPrime, that are used to keep the manifold tubes moist and free from clogging.

• Support for the following languages: English, French, German, Italian, and Spanish. To switch to a different language, press these keys from the Main Menu: UTIL > SETUP > LANGUAGE.



Compatibility with the BioStack™ Microplate Stacker

The MicroFill™ is compatible with BioTek’s BioStack Microplate Stacker. The basecode software of the MicroFill has been modified so that its normal operating displays include the BioStack options. Upgrade kits are available for customers who purchased their MicroFill before modifications to the basecode. (The MicroFill’s basecode version must be 1.10 or greater to operate with the BioStack.)

Controlled serially by the MicroFill, the BioStack can rapidly and systematically transfer microplates one at a time, to and from the dispenser, and includes:

• Removable stacks (one input and one output) that can hold up to 30 microplates each.

• Optional restacking of plates to maintain correct sequencing.

• The ability to continue processing a stack of plates following the aborting/ failure of one plate.

• The ability to pause processing to add more plates to the input stack or to remove some from the output stack.

If you have purchased the BioStack to operate with the MicroFill, refer to the BioStack Operator’s Manual for instructions on configuring the dispenser to run with the BioStack. If you are interested in purchasing the BioStack, contact your local BioTek dealer for more information.

Hardware Features | 5


Hardware Features

• 2-line x 24-character LCD display.

• Membrane keypad with 25 alphanumeric keys.

• Dispenses to 96-well microplates with 0.355" (9 mm) well centers.

• Dispenses to 384-well microplates with 0.177" (4.5 mm) well centers.

• Dispenses to 24-well microplates with 0.582" (14.78 mm) well centers.

• External 24-volt power-supply is compatible with 100 – 240 V~; 50 - 60 Hz.

• One serial RS-232 COM port (25-pin male connector).

• Two 2-liter supply bottles; the autoclavable instrument has two 1-liter glass supply bottles.

• Controllable flow rates and volumes provided by a positive displacement syringe pump. Settings are adjustable via the onboard software, for low- to high-velocity dispensing.

• Stepper motors provide accurate and repeatable carrier and manifold positioning.

• Dispenses to flat, round and “V” bottomed strips and plates in standard and deep-well depths.

• Carrier is easily removed for cleaning.

• Priming/soaking trough built into the carrier. A prime trough drain kit is supplied as an accessory.

• Mist shield protects the user from aerosols.



Package Contents

Package contents and part numbers are subject to change. Please contact BioTek Customer Care with any questions.

• Power supply (PN 61062)

• Power cord (PN varies according to country of use)

• Priming plate (PN 7142002)

• RS-232 serial cable (PN 75053)

• Operator’s Manual (PN 7141000)

• 16-channel manifold (PN 7140519 for nonautoclavable model; PN 7140508 for autoclavable model)

• Two thumbscrews for attaching the manifold (PN 12174)

• Microplate carrier (PN 7140501)

• Microplate adaptor (PN 7140504)

• Two 2-liter supply bottles (PN 4070515) (autoclavable instrument includes two 1-liter glass supply bottles, PN 7140511)

• Supply tubing set (PN 7140505 for non-autoclavable; 7140510 for autoclavable)

• Mist shield (PN 4070517)

• Two thumbscrews for attaching the mist shield (PN 19965)

• Stylus (PN 2872304)

• Prime trough drain kit (PN 7140006)

• Autoclavable instrument includes 3/32” (2.38 mm) hex wrench (PN 48570)

Optional Accessories | 7


Optional Accessories

Accessory availability and part numbers are subject to change. Please contact BioTek Customer Care if you have any questions, or visit www.biotek.com and use the Accessories search tool.

• “8-96” autoclavable manifold for 96-well half-area and PCR plates (BioTek part number 7140515S)*

• “8-24” autoclavable manifold for 24-well plates (BioTek part number 7140518S)*

• PCR plate adaptor (BioTek part number 6002076)**

• Supply tubing set for dispensing organic solvents, such as DMSO and Acetonitrile (BioTek part number 7140514)*

• Spare autoclavable syringe head with piston (BioTek part number 7140516)*

• Spare standard 16-channel manifold (BioTek part number 7140519S)

• Spare autoclavable 16-channel manifold (BioTek part number 7140508S)*

• Spare standard tubing set (BioTek part number 7140505)

• Spare autoclavable tubing set (BioTek part number 7140510)*

• ActiveX® programmer’s package (BioTek part number 7140202)

* For use with autoclavable model AF1000A only

** For use in conjunction with part number 7140515S



Specifications

Labware

Microplates: 96-well, 384-well, standard, low profile, or deep-well depth Standard 24-well, with outside dimensions of 5.030” ± 0.010” x 3.365” ± 0.010” (127.76 mm ± 0.25 mm x 85.47 mm ± 0.25 mm)

Microstrips: 1 x 8

Microwells: Flat, round, “V” bottom, PCR, or half-area

Dispense Precision and Accuracy Performance

The MicroFill™ Microplate Dispenser is factory-tested using dispense volumes of 80 µl and 20 µl.

Precision: ≤ 2%CV typical when dispensing 100 µl of deionized water with FD&C #1 blue or #5 yellow dye, at dual wavelength using the appropriate wavelength for the dye < 5%CV (typical precision at 20 µl per tube) < 10%CV (typical precision at 5 µl per tube)

Accuracy, 16-Channel Manifolds:

384-Well Dispense 1 µl for volumes of 5 to 100 µl, or 1% for all other volumes 96-Well Dispense 2 µl for volumes of 10 to 200 µl, or 1% for all other volumes

Accuracy, 8-Channel Manifolds:

96-Well Dispense 2 µl for volumes of 10 to 20 µl 1.5 µl for volumes of 21 to 40 µl 1 µl for volumes of 41 to 100 µl 1% typical for all other volumes when dispensing deionized water into 96-well plates 24-Well Dispense 4 µl for volumes of 20 to 40 µl 3 µl for volumes of 41 to 80 µl 2 µl for volumes of 81 to 200 µl 1% typical for all other volumes when dispensing deionized water into 24-well plates

Specifications | 9


Hardware & Environmental

Manifold Types:

16-channel non-autoclavable 16-channel autoclavable 8-channel autoclavable for dispensing to 96-well plates (“8/96”) 8-channel autoclavable for dispensing to 24-well plates (“8/24”)

User Interface:

2-line x 24 character LCD screen 25 alphanumeric soft keys

Power Supply:

The instrument is operated from a 24-volt external power supply that is compatible with 100-240 V~; 50-60 Hz.

Dimensions: Instrument only: 18 in. x 15 in. x 7 in. (46 cm x 38 cm x 18 cm)

Weight: 20 lbs. (8.9 kg)

Operating Conditions:

10° - 40°C (50° - 104°F)

Relative Humidity:

The instrument should be operated in a noncondensing humid environment having a maximum relative humidity of 80% at temperatures up to 31° decreasing linearly to 50% relative humidity at 40°C.



Chapter 2

Installation

This chapter includes instructions for setting up the MicroFill™ Microplate Dispenser, and for installing its components.

1: Unpack and Inspect the Instrument .................................... 12 2: Select an Appropriate Location ........................................... 12 3: Install the MicroFill’s Components ....................................... 13

Microplate Carrier ............................................................ 13 Microplate Adaptor ........................................................... 13 Manifold ......................................................................... 14 Syringe Head .................................................................. 15 Check Valves, Tubes, and Bottles ....................................... 16 Prime Trough Drain Kit ..................................................... 19 Mist Shield ...................................................................... 20 Power Supply and Cords ................................................... 21 Data Communication ........................................................ 21 Operating the MicroFill™ with the BioStack .......................... 21

4: Verify Performance ........................................................... 22 Repackaging and Shipping Instructions ................................... 23

Before shipping the instrument: ......................................... 23 Repackaging the instrument and its accessories: .................. 23

12 | Chapter 2: Installation


1: Unpack and Inspect the Instrument

Important! Save all packaging materials. If you need to ship the instrument to BioTek for repair or replacement, you must use the original packing materials. Using other forms of commercially available packing materials, or failing to follow the repackaging instructions, may void your warranty.

If the original packing materials have been damaged, replacements are available from BioTek.

Inspect the shipping box, packaging, instrument, and accessories for signs of damage.

If the MicroFill is damaged, notify the carrier and your manufacturer’s representative. Keep the shipping cartons and packing material for the carrier’s inspection. BioTek will arrange for repair or replacement of your instrument immediately, before the shipping-related claim is settled.

See page 23 for complete packaging and shipping instructions.

2: Select an Appropriate Location

Install the Dispenser on a level surface in an area where ambient temperatures between 10°C (50°F) and 40°C (104°F) can be maintained.

The Dispenser is sensitive to extreme environmental conditions. Avoid excessive humidity. Condensation directly on the sensitive electronic circuits can cause the instrument to fail internal self-checks. See the environmental specifications in Chapter 1.

3: Install the MicroFill’s Components | 13


3: Install the MicroFill’s Components

Important! Refer to Figure 1 on page 14 when installing the microplate carrier, microplate adaptor, and dispense manifold.

Microplate Carrier

• Hold the microplate carrier over the rail so that its priming trough is closest to the rear of the dispenser.

Note: The priming trough is used if a short prime is programmed as a part of a dispense program, catching any drips from the manifold that may fall when the syringe pump is homed. It also serves as an alternate priming location when the MicroFill™ is integrated into a robotic system.

• Line up the pin on the underside of the carrier with the slot on the carrier transport.

• Snap the two carrier clips onto the carrier transport rail. The pin should sit in the slot.

Microplate Adaptor

• Insert the microplate adaptor into the carrier. Ensure that the adaptor is fully seated in the carrier.

Note: The microplate adaptor must be in place when using standard height microplates and when priming the dispenser. For dispensing into deep-well plates, the microplate adaptor must be removed.

Priming Plate The supplied priming plate is used when running Prime and Maintenance programs (see “Prime Programs” in Chapter 3). The priming plate sits inside the microplate adaptor, and it has a partition that divides the plate into two compartments. See Figure 9 in Chapter 3.

• When priming the instrument, the priming plate should be oriented with the large compartment under the manifold.

• When running a maintenance program, the priming plate should be oriented with the small compartment under the manifold.



Manifold

• Orient the manifold so that the tubes are pointed down, and the fitting is pointed toward the rear of the dispenser.

• Slide the manifold gently down over the two support pins closest to the front of the dispenser.

• Insert the thumbscrews (2). Do not over-tighten.

Figure 1: Installing the MicroFill’s components

Rail

Keypad

Priming Trough

LevelingScrews

Glide Strip (Do not remove)

Carrier Transport

MicroplateCarrier

Manifold

Microplate Adaptor

Thumbscrews (2)

FRONT

REAR



Syringe Head

For autoclavable versions of the instrument, model AF1000A, only.

To install the syringe head onto the syringe drive at the rear of the instrument:

1. With the flat side of the shaft facing up, slide the syringe piston shaft into the piston holder until it stops.

2. Use the 3/32” (2.38 mm) hex wrench to tighten the setscrew down onto the flat side of the shaft.

3. Push the syringe head onto the alignment pins and use the hex wrench to attach the two mounting screws.

4. A small magnet is provided for mounting the hex wrench to the base of the Dispenser for storage. Choose a convenient location for the magnet and attach the wrench to it.

Figure 2: Dispenser rear panel, syringe head installation (autoclavable version)

Setscrew

Piston holder (Syringe drive component)

Syringe head

Mounting screws (2)

Syringe piston shaft, flat side up



Check Valves, Tubes, and Bottles

Important! Review Figure 3 through Figure 5 on the following pages, as well as the diagram on the back of the dispenser. Identify the various tubes and bottles that came with your dispenser.

The supply bottles have Luer fittings. Finger-tighten only!

Check Valves Two check valves are supplied; note the flow direction arrows. Connect the check valves to the syringe pump’s top and bottom ports. Compare the flow direction arrows with those shown below.

Figure 3: Dispenser rear panel, check valve installation

Note: If the check valves are replaced, it may be necessary to recalibrate the syringe backlash to achieve optimum accuracy performance. See “Calibrating the Backlash” in Chapter 4 for more information.

Syringe pump top port, supply outlet

Serial port connector

Power cord connector

Syringe pump bottom port, supply inlet

Lower check valve; note direction of flow

Upper check valve; note direction of flow



Note: It is difficult to see the flow direction arrows on the autoclavable check valves. The valves are made of a translucent plastic and the flow direction can be determined by looking through the wall of the outer shell of the check valve. One end of the check valve has two red/orange o-rings; the other end has one red/orange o-ring and a gray spring. The direction of flow is from the end having one o-ring to the end having two o-rings. See below.

Figure 4: Autoclavable check valve, detail

Supply Tubes Connect the check valves as shown in Figure 3. The upper check valve attaches to the dispense manifold (shorter tube); the lower check valve attaches to the fluid supply bottle (longer tube). See Figure 5.

Bottles • Rinse all bottles with deionized or distilled water before using. This rinsing

eliminates particles that may have entered during packing or unpacking.

• Place the supply bottles on the same horizontal plane as the dispenser. This ensures optimum pump performance.

Final Check • Verify that the tubing was not crimped during installation.

• If the mounted manifold tilts forward or backward, make sure that the tubing to the manifold is not being pulled tight or off to the side.

Direction of flowon check valves

One o-ring on this side, red/orange in color

Two o-rings on this side, red/orange in color



Luer Fitting

Figure 5: Tubing connections

Note: The dispenser’s top shroud is removed from this figure for clarity only. While it is acceptable to remove the mist shield (see page 20), do not attempt to remove the top shroud which protects the dispenser’s internal components.

Short Supply Tube

Long Supply Tube

Supply Bottle

On/Off Switch Microplate Carrier



Prime Trough Drain Kit

Refer to the figure below when installing the prime trough drain kit.

1 . Remove drain plug and install the threaded fitting.

2. Move carrier all the way forward and install tubing onto the barbed fitting on the back of the priming trough.

3. Route tubing as shown with a little spare tubing in the back. Apply the clamp approximately as shown. Then place the tubing into the clamp.

Priming Trough

Drain Plug

ClampTubing

Priming Trough Drain Instructions

ThreadedFitting

Figure 6: Priming Trough Drain Kit, installation instructions



Mist Shield

1. Insert the two thumbscrews into the slots on the dispenser’s left side. Do not tighten completely.

2. Hold the mist shield parallel to the dispenser’s left side, with the door toward the front of the dispenser.

3. Slide the mist shield down so that the two slots fit over the thumbscrew shafts.

4. Fit the two pins into the slots on the dispenser’s top left side.

5. Tighten the thumbscrews.

Figure 7: Attaching the mist shield

Pins (2)

Mist shield

Thumbscrews (2)

Top shroud (do not remove)



Power Supply and Cords

The MicroFill™ uses an external 24-volt power supply that is compatible with 100-250 V~; 50-60 Hz. Connect the power cord to the power supply, then connect the power supply’s output plug to the 24-VDC connector on the rear of the instrument. Plug the 3-prong end of the power cord into an appropriate power receptacle.

Warning! Power Rating. The MicroFill™ must be connected to a power receptacle that provides voltage and current within the specified rating for the system. Use of an incompatible power receptacle may produce electrical shock and fire hazards

Data Communication

Serial Port Communications with Other Devices The MicroFill™ has a 25-pin serial (RS-232) port located on the rear panel of the instrument (see Figure 3). The serial port allows the dispenser to communicate with the BioStack™ Microplate Stacker, to receive data from a computer for purposes of factory or dealer service, and/or to be operated using an ActiveX® interface (contact your BioTek representative for more information on obtaining a programmer’s package).

Operating the MicroFill™ with the BioStack

Alignment hardware and serial cable connections need to be installed to operate the MicroFill with the BioStack™ Microplate Stacker. Refer to your BioStack Operator’s Manual for installation instructions.



4: Verify Performance

Before using the MicroFill™ for the first time, verify that it is operating properly by turning the dispenser on. The power switch is located on the dispenser’s left side.

Each time the dispenser is turned on, it automatically performs a system self-test. This test checks the carrier, manifold lift positioning, and syringe pump positioning and operation. If the test passes, the Main Menu appears and the dispenser is ready for use.

If the self-test fails, the dispenser will “chirp” and display an error code. Press the Stop button on the keypad and then look up the code in Appendix A, Error Codes.

Note: You can also run this test from the dispenser’s front panel, by selecting UTIL > TESTS > SELFCHK.

For information on performance verification and IQ/OQ/PQ procedures, see Chapter 5, Instrument Qualification.

Important! Before operating the dispenser, review the General Usage Guidelines in Chapter 6. These guidelines include necessary steps to perform before running any program, information on performing preventive maintenance,

Repackaging and Shipping Instructions | 23


Repackaging and Shipping Instructions

If you need to ship the MicroFill to BioTek for service or repair, be sure to use the original packing materials. Other forms of commercially available packing are not recommended and can void the warranty.

If the original packing materials have been damaged or lost, contact BioTek Customer Service for replacement materials.

If the MicroFill has been exposed to potentially hazardous material, decontaminate it to minimize the risk to all who come in contact with the Dispenser during shipping, handling and servicing.

Decontamination prior to shipping is required by the U.S. Department of Transportation regulations.

Before shipping the instrument:

• Decontaminate the dispenser and its accessories as necessary (see Chapter 4).

• Obtain a Return Materials Authorization (RMA) number from BioTek TAC. Mark this number on the outside of the shipping box.

• Include instructions regarding what is required of the BioTek Service Department. Clearly state whether the unit requires calibration, cleaning, periodic maintenance, warranty work, and/or repair.

• Provide BioTek with the name and telephone number of a person who may be contacted if questions arise.

• Insure the instrument for full value.

Repackaging the instrument and its accessories:

Refer to the Customer Packing Instructions in Appendix D.



Chapter 3

Operation

This chapter includes instructions for operating the MicroFill™ Microplate Dispenser and its on-board software.

Turn On/Off the MicroFill™ .................................................... 26 Keypad Description ............................................................... 26 Operation with the BioStack ................................................... 28 Main Menu .......................................................................... 28 Program Types .................................................................... 29 Prime Programs ................................................................... 29

Priming Considerations ..................................................... 29 Define Prime Program ...................................................... 31 Run Prime Program .......................................................... 31 Edit Prime Program .......................................................... 32 Copy Prime Program ........................................................ 32 Delete Prime Program ...................................................... 32

Dispense Programs ............................................................... 33 Define Dispense Program .................................................. 33 Run Dispense Program ..................................................... 35

Soak Programs .................................................................... 35 Link Programs ..................................................................... 36

Define Link Program ......................................................... 36 Utility Menu Options ............................................................. 37

TESTS ............................................................................ 37 SETUP ............................................................................ 37 AUTOPRIME .................................................................... 38

26 | Chapter 3: Operation


Turn On/Off the MicroFill™

To turn the MicroFill™ on, press the On/Off switch on the dispenser’s right side. The keypad will remain inactive while the dispenser performs a self-test. If the test passes, the Main Menu will appear and the dispenser is ready for use.

If the self-test fails, the dispenser will “chirp” and display an error code. Press the Stop key and look up the code in Appendix A, Error Codes. See also Chapter 6, Troubleshooting.

To turn the MicroFill™ off, press the On/Off switch on the dispenser’s right side.

Note: To rinse, soak, or decontaminate the dispenser before turning it off, see Chapter 4, Preventive Maintenance.

Keypad Description

The MicroFill features a 25-key keypad and a 2-line x 24-character LCD display as the user interface. The keypad is shown below.

Figure 8: Keypad

µFill Reagent Dispenser RUN DEFINE MAINT UTIL LCD

Softkeys: Press a key to choose a menu function

Press the left and right arrows to move the cursor in the LCD display. Press Options to scroll through options or the alphabet. Press Shift+Options to scroll backward through options or the alphabet.

Menu functions

Keypad Description | 27


Select Menu Functions

The menu functions appear in the bottom (second) line of the display. To make a selection, press the SoftKey on the keypad below the menu function.

Scroll Through Options

Certain functions, such as entering a program name or selecting a program type, offer a set of options from which to make a selection. To view the different options, press the Options key or the Shift + Options key combination. Press the ENTER key to select the current option.

Enter Program Names

NAME: Dispense 96

- / : _

Each MicroFill™ program requires the entry of a unique name, using up to 16 alphanumeric characters. To enter a program name via the Dispenser keypad, press Shift + key A-H, or scroll through the alphabet with the Options key for A-Z. Shift + Options reverses direction. Use the right (forward) or left (back) arrows to move the cursor within the display.

Press the ENTER key to store the completed program name and continue. If the program name already exists, the dispenser software will require you to enter a unique name. This protects dispenser programs from inadvertently being overwritten.

The NAME display offers four symbols that can be used in a program name: dash (hyphen), forward slash, colon, and underscore. These symbols appear in the LCD as SoftKey choices (see “Select Menu Functions” above). To include a symbol within a program name, press its corresponding SoftKey.

Move Backward Through the Menus

To move to a previous menu, press the Previous Screen key.

Clear Entry

To delete an entry, press the CLEAR key.

Save Program

After defining a dispenser program, press the Main Menu key, and then press YES to save the program.

Start/Stop Program

Press the START key to begin running a dispenser program. Press the STOP key to stop running (abort) a program.



Operation with the BioStack

If you have purchased BioTek’s BioStack Microplate Stacker to operate with the MicroFill, refer to the instructions in your BioStack Operator’s Manual for setting up and configuring the dispenser to operate with the BioStack.

You can run Dispense and Link programs on the MicroFill with or without the BioStack attached. The Prime and Maintenance programs can only be run on the MicroFill without the BioStack.

Main Menu

Following successful power-up of the MicroFill™, the Main Menu appears:

µFill Reagent Dispenser

RUN DEFINE MAINT UTIL

The following options are available through the Main Menu:

Under RUN:

SELECT PROGRAM TYPE:

DISP PRIME SOAK LINK

Under DEFINE:

SELECT ACTION:

CREATE EDIT COPY DELETE

Under MAINT:

SELECT MAINT PROGRAM: XX

<program name>

Under UTIL:

SELECT UTILITY:

TESTS SETUP AUTOPRM

The RUN, DEFINE, and UTILITY options are described in this chapter.

See Chapter 4, Preventive Maintenance for information on the MAINTenance options.

Program Types | 29


Program Types

The following program types can be defined and run through the MicroFill™’s keypad:

• Dispense: Define parameters such as Number of Strips, Flow Rate, and Dispense Volume, for dispensing fluid to the microwells. See page 33.

• Prime: Define routines for priming the fluid lines and manifold before dispensing begins. See page 29.

• Soak: Define a soak duration for inclusion in a Link program. See page 35.

• Link: A series of Dispense and/or Soak programs “linked” together to run sequentially and automatically. See page 36.

Prime Programs

Priming Considerations

Adequate priming is necessary to consistently achieve within-specification accuracy and precision from the dispenser. We recommend that you use a two-step technique to prime the dispenser. This requires running a Prime program and then a Dispense program. First, insert the priming plate and run a Prime program that primes with 20 ml of the solution to be dispensed. We recommend using a prime Rate of 5. Next, with the priming plate still in place, run a Dispense program that dispenses 20 μl per well to 24 columns of a 384-well microplate at rate 2. This will pump any residual air out of the check valves. The dispenser is now ready to run a dispense program. The default programs New_Buffer_Prime and accuracy_test may be used for this purpose, or you may create your own programs.

When a Prime program is run, the priming plate must be in place in the microplate adaptor. The priming plate has an internal partition that divides its interior into two compartments (see Figure 9 on the next page).

• When priming the instrument, the priming plate should be oriented with the large compartment under the manifold.

• When running a Maintenance program, the priming plate should be oriented with the small compartment under the manifold.



Figure 9: Priming plate

The following sections discuss how to define, run, edit, copy, and delete Prime programs.

A prime trough drain kit is supplied (BioTek part number 7140006) for the installed priming trough. With the drain kit in place, it is possible to perform non-soak primes using the installed (fixed) priming trough instead of the removable priming plate. The prime program, TROUGH_PRIME, must be run for standalone primes using this trough. It is possible to edit the parameters of this program.

Internal partition Small compartment – place under the manifold while running a maintenance program

Large compartment – place under the manifold while priming

Prime Programs | 31


Define Prime Program

To define a Prime program, follow the menu path shown here. Some of these items are options and some are prompts. The options are described below.

DEFINE > CREATE > PRIME > NAME > PRIME VOLUME > PRIME FLOW RATE > SOAK AFTER PRIME? > SOAK DURATION > OK TO SAVE PROGRAM?

Define Prime Program Options Options Actions

Name Enter a unique name for the Prime program. See “Enter Program Names” on page 27 for instructions.

Prime Volume Specify the volume of the solution or rinse used to prime the dispense tubes. The volume range is 1 to 200 ml.

Prime Flow Rate Specify the rate at which the priming fluid is pumped into the dispense tubes. The flow rate options range from 1 to 5, where 1 = Slowest, 5 = Fastest. The dispenser display shows the corresponding µl/well/sec value for each option.

Soak After Prime Yes/No moves the manifold to a lower position where the dispense tubes can be soaked in the small compartment of the priming plate. Useful for overnight maintenance and decontamination programs. See Chapter 4.

Soak Duration Specify the number of minutes and hours to soak. The range is 1 minute to 18 hours. This option is useful for Maintenance programs; see Chapter 4.

OK to Save Program? Select YES to save the program.

Run Prime Program

To run a Prime program, follow the menu path shown here. Some of these items are options and some are prompts. The options are described below.

RUN > PRIME > SELECT PRIME PROGRAM > INSERT PRIMING PLATE AND PRESS <START> KEY > PRIME PROGRAM RUNNING. PRESS <STOP> KEY TO QUIT > PRIME PROGRAM COMPLETE.* NEW REPEAT

*If NEW is pressed at the PRIME PROGRAM COMPLETE screen, a new program can be selected and run. If REPEAT is pressed, the same program will be rerun.



Edit Prime Program

To change a Prime program, follow the menu path shown here. Some of these items are options (see the previous page), and some are prompts.

DEFINE > EDIT > PRIME > SELECT PRIME PROGRAM > Edit the Program Parameters > MAIN MENU KEY > OK TO SAVE PROGRAM?

Copy Prime Program

To copy a Prime program, follow the menu path shown here. Some of these items are options and some are prompts. The options are described below.

DEFINE > COPY > PRIME > SELECT PRIME PROGRAM > NAME > OK TO COPY?

Copy Prime Program Options Options Actions

Select Prime Program Select the Prime program you wish to copy.

Name Enter a unique name for the new Prime program.

OK to Copy? Select YES to copy the program.

Delete Prime Program

To delete a Prime program, follow the menu path shown here. Some of these items are options and some are prompts. The options are described below.

DEFINE > DELETE > PRIME > SELECT PRIME PROGRAM > OK TO DELETE?

Delete Prime Program Options Options Actions

Select Prime Program Select the Prime program you wish to delete.

OK to Delete? Select YES to delete the program.

Dispense Programs | 33


Dispense Programs

Before running a Dispense program, be sure to adequately prime the dispenser. Failure to adequately prime the dispenser may leave residual air in the fluid lines and check valves that will be dispensed into the wells. See “Priming Considerations” on page 29.

This section discusses how to define and run Dispense programs. Dispense programs may also be edited, copied, and deleted. These actions are identical to those used for editing, copying, and deleting Prime programs and are not repeated here (see page 32).

Define Dispense Program

To define a Dispense program, follow the menu path shown here. Some of these items are options and some are prompts. The options are described below.

DEFINE > CREATE > DISP > NAME > PLATE TYPE > FIRST STRIP TO DISPENSE > NUMBER OF STRIPS > DISPENSE VOLUME > DISPENSE FLOW RATE > DISPENSE HEIGHT > PLATE CLEAR HEIGHT > HORIZONTAL DISP POS > PRIME BEFORE START? > PRIME VOLUME > PRIME FLOW RATE > OK TO SAVE PROGRAM?

Define Dispense Program Options Options Actions

Name Enter a unique name for the Dispense program, using up to 16 alphanumeric characters. See “Enter Program Names” on page 27 for instructions.

Plate Type (16-channel manifold only) Select a Plate Type of 96 if dispensing into 8-well strips or 96-well plates, or 384 if dispensing into 384-well plates.

First Strip to Dispense Specify the first strip to dispense into. The range is 1 to 6 for 24-well plates, 1 to 12 for 96-well plates, and 1 to 24 for 384-well plates.

Number of Strips Specify the number of strips to dispense into. The range is 1 to 6 for 24-well plates, 1 to 12 for 96-well plates, and 1 to 24 for 384-well plates.

Dispense Volume Specify the volume of solution to dispense per microwell, per dispense cycle. The volume range is 10 to 3000 µl/well for 96-well plates, 5 to 1500 µl/well for 384-well plates, and 20 to 6000 µl/well for 24-well plates.



Dispense Flow Rate Specify the rate at which the fluid is dispensed from the tubes. The flow rate options range from 1 to 5, where 1 = Slowest, 5 = Fastest. The Dispenser display shows the corresponding µl/well/sec value for each option. Not all dispense rates are valid for all dispense volumes. Only valid dispense flow rates will be displayed for the volume selected. Tables for valid flow rates per volume are contained in Appendix C, Dispense Flow Rates. The user must choose a valid flow rate to exit this screen.

Dispense Height Dispense Height represents the height between the bottom of the dispense tubes and the microplate adaptor surface on which the plate rests, during a dispense. The Dispense Height options range from 51 to 195. The Dispenser display shows the corresponding measurement in millimeters for each option. 51 is the closest to the adaptor surface, and 195 is the furthest from this surface.

Plate Clear Height The Plate Clear Height represents the height between the bottom of the dispense tubes and the adaptor surface on which the plate rests, after a dispense and whenever the carrier is moving. The Plate Clear Height options range from 51 to 195. The Dispenser display shows the corresponding measurement in millimeters for each option.

Horizontal Disp Pos Horizontal Dispense Position represents the position of the dispense tube in relation to the center of the microwell. The Horizontal Disp Pos options range from: -80 to 80. The Dispenser display shows the corresponding measurement in millimeters for each option. A negative offset (-80 to -1) moves the dispense tubes away from the front edge of the well. A positive offset (1 to 80) moves the dispense tubes toward the front edge of the well. A setting of 0 indicates no offset (0.000 mm).

Prime Before Start? Select YES to prime the dispense tubes before starting the Dispense program. Note: Prime Before Start is intended to be a small (2 ml or less) volume pre-prime. It occurs in the priming trough that is integral to the carrier. The maximum volume of this trough is 16 ml. Unless the optional prime trough drain kit is installed, the trough must be emptied by the user when it becomes full.

Prime Volume Specify the volume of the buffer or rinse used to prime the dispense tubes. The volume range is 5 to 1500 μl/well.


Press Main Menu to end Select YES to save the program.

Soak Programs | 35


Run Dispense Program

To run a Dispense program, follow the menu path shown here. Some of these items are options and some are prompts. The options are described below.

RUN > DISPENSE > SELECT DISPENSE PROGRAM > MANIFOLD NOT PRIMED?* > FIRST STRIP TO DISPENSE > NUMBER OF STRIPS > INSERT MICROPLATE AND PRESS <START> KEY > DISPENSE PROGRAM RUNNING. PRESS <STOP> KEY TO QUIT > DISPENSE PROGRAM COMPLETE.** NEW REPEAT

* MANIFOLD NOT PRIMED? appears only after instrument power-up or after a Maintenance program has been run. Choose ABORT to prime or PROCEED to continue without priming.

** If NEW is pressed at the DISPENSE PROGRAM COMPLETE screen, a new program can be selected and run. If REPEAT is pressed, the same program will be rerun.

Run Dispense Program Options Options Actions

Dispense program Select a Dispense program.

Prime? Select a Prime program to run before starting the Dispense program.

First Strip to Dispense Specify the number of the microwell strip to start dispensing: 1 to 12 for 96-well plates 1 to 24 for 384-well plates 1 to 6 for 24-well plates

Number of Strips Specify the total number of microwell strips to be dispensed: 1 to 12 for 96-well plates 1 to 24 for 384-well plates 1 to 6 for 24-well plates

Soak Programs

Soak programs are defined, edited, copied, and deleted in the same way as Prime and Dispense programs. Soak programs are unique, however, because they cannot be run as stand-alone programs. A Soak program can only be run as one of a series of programs in a Link program.

• A Soak program cannot be run independently; it must be selected as part of a Link program (see next section).

• A Soak program is essentially a delay built into a Link program.



Link Programs

A Link program is a series of Dispense and/or Soak programs linked together, and is run and deleted in the same way as other program types. A Link program cannot be copied or edited, but its contents can be viewed by selecting:

DEFINE > EDIT > PRIME > SELECT PRIME PROGRAM > View the Program Parameters

• Predefined Dispense and Soak programs can be “linked” together and run sequentially and automatically.

• A Link program can contain up to 30 pre-defined programs, all of which must be defined for the same manifold type and plate type (“96” or “384”, for 16-channel manifold only).

• Once defined, a Link program can be viewed, run, or deleted. It cannot be edited or copied.

Define Link Program

To define a Link program, follow the menu path shown here. Some of these items are options and some are prompts. The options are described below.

DEFINE > CREATE > LINK > NAME > PLATE TYPE > SELECT LINK 01 (02, 03, up to 30) TYPE > SELECT PROGRAM > OK TO SAVE PROGRAM?

Define Link Program Options Options Actions

Name Enter a unique name for the Link program. See “Enter Program Names” on page 27 for instructions.

Plate Type (Applies to 16-channel manifold only) Select a Plate Type of 96 to link programs defined for 8-well strips or 96-well plates, or 384 to link programs defined for 384-well plates.

Select Link (n) Type Select either Dispense or Soak.

Select Program Select each Dispense and/or Soak program to be included in the Link program. Programs will run in the order in which they are selected. Note: Only programs with the selected Plate Type will be accessible for inclusion in the Link program.

Press Main Menu to end Select YES to save the program.

Utility Menu Options | 37


Utility Menu Options TESTS

Self-Check Each time it is turned on, the MicroFill™ automatically runs a self-check to verify system components. The self-check can also be run from the dispenser’s front panel by pressing UTIL > TESTS > SLFCHK. The self-check includes verification of the syringe, manifold, and carrier movement; and the software checksum.

If the test completes successfully, the Main Menu appears and the dispenser is ready for use. If the test fails, the dispenser will “chirp” and display an error code. Press the STOP key and then look up the error code in Appendix A, Error Codes.

Configuration Information (CHKSUM) The MicroFill stores the basecode software part number, version number, and checksum value. This information is important if you need to contact BioTek’s Technical Assistance Center. To view this information, start at the Main Menu and select UTIL > TESTS > CHKSUM.

SETUP

Language Your MicroFill’s display may be configured for one of several languages, including English, French, German, Italian, and Spanish. To change the language, follow the menu path shown below:

UTIL > SETUP > LANGUAGE > ENGLISH, FRENCH, GERMAN, ITALIAN, or SPANISH > MAIN MENU KEY

Manifold Your autoclavable MicroFill may be configured for one of three manifolds: the standard 16-channel manifold, the “8-96” manifold, or the “8-24” manifold.

When physically changing manifolds, you must specify the manifold in use in the software. To change the manifold setting, follow the menu path shown below:

UTIL > SETUP > MANIFOLD > 16ch, 8-96ch, or 8-24ch > MAIN MENU KEY



BIOSTACK UTIL > SETUP > BIOSTACK > CONF, ALIGN, or VERIFY

The BIOSTACK UTILITIES screen will appear when BIOSTACK is selected. The MicroFill™ can then be configured for proper operation with the BioStack by selecting the CONF (Configuration), ALIGN (Alignment), and VERIFY (Verification) utilities. Refer to your BioStack Operator’s Manual for detailed instructions.

AUTOPRIME

Frequent priming keeps the dispenser’s manifold tubes moist and helps to prevent them from clogging. The MicroFill provides an AUTOPRIME self-priming program, which you can enable, disable, or edit (see below). If enabled, the program will run automatically after the dispenser is idle for the specified period of time. See “AutoPrime” in Chapter 4 for more information.

• To enable the AutoPrime feature, follow the menu path shown below and enter an autoprime interval from 1 to 600 minutes.

UTIL > AUTOPRIME > ENABLE AUTOPRIME? > YES

• To disable the AutoPrime feature, follow the menu path shown below:

UTIL > AUTOPRIME > ENABLE AUTOPRIME? > NO

• To interrupt AutoPrime, press the STOP key.

Editing the AutoPrime Program The AutoPrime Prime program can be edited to change the length of the soak period, eliminate the soak period, or to change the interval between AutoPrime cycles. To edit this program, follow the menu path shown below.

DEFINE > EDIT > PRIME > SELECT PRIME PROGRAM > Edit the Program Parameters (see below) > MAIN MENU KEY > OK TO SAVE PROGRAM?

AutoPrime Program Options Options Actions Prime Volume Specify the volume of the buffer or rinse used to prime the

manifold. The volume range is 1 to 200 ml. Do not specify more volume than can be safely contained in the priming plate during idle periods.


Utility Menu Options | 39


Options Actions Soak after Prime? Select YES to soak the manifold tubes after they have

been primed. The priming solution is dispensed to the small compartment of the priming plate. The manifold is lowered into the plate and the tubes are submerged in the priming fluid. After soaking, the manifold is raised to its home position.

Soak Duration Soak Duration represents the time to allow the dispense tubes to be soaked in priming solution, rinse, or disinfectant. The range is from 1 minute to 18 hours.

AutoRinse By connecting a rinse bottle to the dispenser, the AutoPrime feature can be used to automatically rinse the dispenser.



Chapter 4

Preventive Maintenance

This chapter provides step-by-step instructions for maintaining the MicroFill™ Microplate Dispenser in top condition, to ensure that it continues to perform to specification.

Overview ............................................................................ 42 Recommended Maintenance Schedule ..................................... 43 Tools and Supplies ............................................................... 43 Maintenance and Prime Programs ........................................... 44 Daily Maintenance ................................................................ 45 Overnight Maintenance ......................................................... 49 Monthly Maintenance ............................................................ 50 Long-Term Storage Maintenance ............................................ 57 Storing the MicroFill Dispenser ............................................... 57 Decontamination .................................................................. 58 Daily/Overnight Maintenance Log Monthly Maintenance Log

42 | Chapter 4: Preventive Maintenance


Overview

Preventive Maintenance (PM) represents a set of procedures that should be performed regularly to maintain equipment in top condition. For example, during normal operation, salt crystals or protein can build up and clog the valves and tubes. Daily and periodic maintenance reduces this problem, maximizes running time, and extends the life of the dispenser.

A routine maintenance schedule consists of:

• Daily Maintenance

• Overnight Maintenance

• Monthly Maintenance

• Long-Term Storage Maintenance

• Decontamination

The MicroFill’s on-board software contains some predefined Maintenance programs, including:

• M_Day_Rinse

• M_Rinse_And_Soak

• M_Overnight_Loop

• M_Long_Shutdown

• M_Decontaminate

These programs are accessible from the dispenser’s Main Menu, under the MAINT option.

The following chart lists preventive maintenance tasks, the frequency with which each task should be performed, the predefined program that should be run, and where to find more information. Maintenance logs are provided at the end of this chapter.

Recommended Maintenance Schedule | 43


Recommended Maintenance Schedule

Tasks Page Purpose/Frequency

Daily Monthly/ As Needed

Before Storage/ Shipment

Daily maintenance Program: M_Rinse_And_Soak 45

Remove protein residuals and fungi growth (if necessary) 46

AutoPrime 48

Overnight maintenance Program: M_Overnight_Loop 49

Clean bottles 50

Clean manifold channels and chambers 50

Clean dispense tubes with stylus 50

Clean plate adaptor, carrier, and transport rail 51

Clean priming plate and trough 51

Clean tubing and check valves 52

Remove liquid from fluid path Program: M_Long_Shutdown 57

Decontaminate the instrument Program: M_Decontaminate 58

Tools and Supplies

• Stylus • Dish soap or other mild cleaner

• ¼” straight screwdriver • Deionized or distilled water

• Lint-free disposable towels • (2) Check valves (PN 68080)

• 70% isopropyl alcohol • (2) Manifold plugs (PN 45090)



Maintenance and Prime Programs

Each predefined Maintenance program runs a predefined Prime program. The charts below show the default prime program parameters. These parameters can be modified.

Maintenance Program M_Day_Rinse

Prime Program: P_Day_Rinse

Prime Volume: 40 ml

Flow Rate: 5

Soak After Prime? No

Maintenance Program M_Rinse_And_Soak

Prime Program: P_Rinse_And_Soak

Prime Volume: 40 ml

Flow Rate: 5

Soak After Prime? Yes

Soak Duration: 5 minutes

Maintenance Program M_Overnight_Loop

Prime Program: P_Rinse_Loop

Prime Volume: 10 ml

Flow Rate: 5


Soak Duration: 12 hours

Daily Maintenance | 45


Daily Maintenance

For all Daily Maintenance tasks, ensure that the:

• supply bottle contains at least 400 ml of rinse solution

• waste bottle is empty

• priming plate is properly inserted in the microplate adaptor.

Rinse and Soak

Daily Maintenance involves flushing the dispenser with an appropriate reagent at the beginning of the day (BioTek recommends using deionized water in the morning).

The M_Rinse_And_Soak program is recommended for the morning. This should be performed on the same day the microplates are dispensed. This procedure helps prevent the dispense tubes from clogging between uses.

The default parameters for the M_Rinse_And_Soak program are listed below.

Maintenance Program M_Rinse_And_Soak

Prime Program: P_Rinse_And_Soak

Prime Volume: 40 ml

Flow Rate: 5


Soak Duration: 5 minutes

To run the M_Rinse_And_Soak program, follow the menu path shown below.

MAINT > Press Options until M_Rinse_And_Soak appears > CONNECT RINSE AND PRESS <START> KEY > MAINTENANCE RUNNING PRESS <STOP> TO QUIT



Removing Protein Residuals and Fungi Growth

Important! Solutions containing proteins, such as bovine serum albumin (BSA), will compromise the dispenser’s performance over time unless a strict maintenance regime is adhered to.

Do not use isopropyl alcohol to flush out BSA.

BioTek recommends performing the following additional Maintenance procedures to thoroughly flush out protein particles and other contaminants from the dispenser’s fluid path, if necessary:

Daily Using an Enzyme-Active Detergent:

1. Mix an enzyme-active detergent according to the manufacturer’s directions to fill a two-liter supply bottle (or a one-liter bottle, for an autoclavable MicroFill™).

2. Run the M_Decontaminate Maintenance program. When the program pauses and displays CONNECT RINSE PRESS <START> KEY, leave the detergent bottle connected and press the Start key to continue. Repeat this sequence until the bottle is empty.

3. Connect a bottle full of deionized water and run the P_Day_Rinse Prime program three times to flush the system.

Using Ethanol:

At the end of the day:

1. Prepare a two-liter solution of 10% ethanol and deionized water (or a one-liter solution for an autoclavable MicroFill™). Do not use isopropyl alcohol.

2. Run the M_Decontaminate Maintenance program.

3. Connect a bottle full of deionized water and flush the system by running either the P_Day_Rinse Prime program (three times), or the M_Overnight_Loop Maintenance program.

Daily Maintenance | 47


Using Deionized Water:

If the dispenser will be idle between plates for longer than 45 minutes, flush the proteins from the system:

1. Fill a supply bottle with deionized water. Connect the bottle to the dispenser.

2. Run the P_Day_Rinse Prime program.

3. Enable AUTOPRIME for 60-minute intervals. At the end of the day:

1. Fill a supply bottle with deionized water. Connect the bottle to the dispenser.

2. Run the P_Day_Rinse Prime program three times.

3. Run the M_Overnight_Loop Maintenance program.

Weekly/Monthly 1. Flush the system with 0.1 – 0.5 N NaOH, followed by neutralization with an

equivalent normality (0.1 – 0.5 N) of HCl.

2. Rinse well with deionized water to remove the HCl.

3. Run the M_Overnight_Loop Maintenance program with deionized water, or run the P_Day_Rinse Prime program three times with deionized water if you plan to use the dispenser immediately.



AUTOPRIME

BioTek recommends enabling the AutoPrime feature to automatically prime (and optionally soak) the dispenser after it has been idle for a user-specified amount of time. The volume, flow rate, and soaking parameters specified in the AUTOPRIME Prime program are used. AutoPrime is particularly useful if the dispenser is used intermittently throughout the day, to keep the fluid lines and manifold in a wetted condition. If you prefer not to enable the AutoPrime feature, BioTek recommends running the M_Rinse_And_Soak program periodically during the day.

If AutoPrime is enabled, the priming plate must be left in the microplate adaptor when the user finishes dispensing a plate. The partition dividing the trough must be placed toward the rear of the instrument.

The AutoPrime feature is disabled by default. To enable it:

1. Select UTIL > AUTOPRIME > YES.

2. Enter the interval from 10 to 600 minutes, to indicate how often you want the AutoPrime Prime program to run.

3. Press ENTER and then return to the Main Menu. AutoPrime is now enabled and priming will occur each time the dispenser has been idle for <interval> minutes.

• To disable the AutoPrime feature, select UTIL > AUTOPRIME > NO.

• To halt the AUTOPRIME program when it is running, press the STOP key.

• To view or edit the AUTOPRIME program parameters, select DEFINE > EDIT > PRIME > AUTOPRIME and then press the Options key to scroll through the parameters you wish to view or edit. The table below shows the AUTOPRIME program parameters and ranges.

Parameter Default Selectable Range

Interval 10 minutes 10 to 600 minutes

Prime Volume 10 ml 1 to 999 ml

Prime Flow Rate 5 1 to 5

Soak After Prime? Yes Yes/No

Soak Duration 11 hours, 50 minutes 1 minute to 18 hours

See “Prime Programs” in Chapter 3 for more information on Prime programs and their parameters.

Overnight Maintenance | 49


Overnight Maintenance

When the dispenser is left overnight or for the weekend, we recommend you run the M_Overnight_Loop Maintenance program. Overnight/multi-day maintenance involves flushing all dispense solution out of the instrument, and then periodically rinsing and soaking the tubes to keep them moist.

• Connect a supply bottle containing deionized water and run M_Day_Rinse to flush any solution out of the dispenser. Run the M_Overnight_Loop program to prime and soak the tubes.

Important! Keep the manifold in a wetted condition to ensure proper operation. BioTek recommends changing the Soak Duration to 18 hours for overnights and weekends when the dispenser is not in use. This will decrease flow problems and is a more trouble-free way to operate the dispenser.

• The M_Overnight_Loop program requires the dispenser to be kept plugged in and turned on. As an alternative, run the M_Rinse_And_Soak Maintenance program and turn off the instrument after the soak begins. This leaves the tubes soaking in the priming plate until the instrument is turned on again.

• The M_Overnight_Loop program will run continuously until the STOP key is pressed. After pressing the STOP key, select YES to home all axes.

To run the M_Overnight_Loop program, follow the menu path shown below.

MAINT > press Options until M_Overnight_Loop appears > CONNECT RINSE AND PRESS <START> KEY > MAINTENANCE RUNNING PRESS <STOP> TO QUIT



Monthly Maintenance

Clean the Bottles and Tubes

• Clean the dispense and rinse bottles and supply tubes with deionized water before the first use, before each refill, and if they have been idle for any length of time.

• Accumulated algae, fungi, or mold may require decontamination; see page 58.

Clean the Manifold

Regular rinsing helps to keep the manifold clean and the dispense tubing clear, and will increase the life of the tubing. Follow the Decontamination procedure starting on page 58 to disinfect the manifold and tubing.

If you suspect a particular problem is related to the manifold (for example, clogged tubes can result in uneven dispensing), you should perform a thorough cleaning of the manifold. To clean the manifold:

1. Turn off and unplug the instrument.

2. Remove the mist shield. See Figure 7 in Chapter 2.

3. Disconnect the tubing from the back of the manifold. See Figure 5 in Chapter 2.

4. Remove the thumbscrews and the manifold. Using a lint-free disposable towel, thoroughly clean the outside of the dispense tubes.

5. Run hot water through the inlet fitting. Check to see if water comes out of all of the dispense tubes. If not, soak the manifold in hot soapy water and repeat. Clean the tubes with the stylus if necessary (see below).

Clean (“Stylize”) the Dispense Tubes

Note: The autoclavable manifold has removable dispense tubes. We do not recommend routinely removing these tubes. In the case of a particularly difficult problem with any one channel, however, a tube may be removed and cleaned individually, or replaced.

The manifold dispense tubes do not need to be “stylized” unless there is a problem. Periodic rinsing helps prevent the need for stylizing. If the previous steps are not completely successful, try the following:

1. Remove the two gray plugs from the ends of the manifold.

2. Tip the manifold on end and flush hot water through this open channel.

3. Using the supplied stylus (PN 2872304), clean the insides of all of the dispense tubes.

Monthly Maintenance | 51


4. Tip the manifold on end and flush hot water through the open channel.

5. Rinse the manifold with deionized or distilled water. Reinsert the gray plugs into the ends of the manifold. Check to see if water comes out of all of the dispense tubes.

6. Remount the manifold and replace the tubing. See Figures 1 and 5 in Chapter 2.

7. Replace the mist shield.

8. Run a Prime program using 40 ml of deionized water.

9. Verify dispense performance visually, or by performing the appropriate Dispense Precision and Accuracy Tests in Chapter 5.

Clean the Plate Carrier

Note: Each plate carrier is specifically aligned to each MicroFill™. Do not adjust the carrier’s nylon screws or interchange the carrier with another.

If liquid has overflowed onto the plate carrier, some crystal buildup may occur and prevent the plate adaptor from seating correctly onto the carrier.

1. Remove the carrier:

• Move it to its full “out” position (turn the dispenser on, wait for the system test to complete, and then turn the dispenser off). Make sure the manifold and tubes are over the priming trough (“home” position).

• Carefully lift the carrier front off the rail, avoiding contact with the manifold tubes.

• Carefully lift the carrier rear off the rail, avoiding contact with the manifold tubes.

2. Clean the carrier using soap and water or 70% isopropyl alcohol.

3. Replace the carrier:

• Hold the microplate carrier over the rail so that the trough is to the rear of the dispenser.

• Line up the pin on the underside of the carrier with the slot on the carrier transport.

• Snap the two carrier clips onto the rail. The pin must sit in the slot.



Clean the Plate Carrier Transport Rail

Important! Do not allow the alcohol or water to seep in between the rail and the plate carrier transport mechanism that rides on the rail. Follow the instructions carefully to prevent this.

If liquid has overflowed onto the stainless steel plate carrier transport rail, some crystal buildup may occur, preventing the carrier from moving properly.

To clean the plate carrier transport rail:

1. Turn off the instrument.

2. Carefully lift the microplate carrier off of its transport mechanism.

3. Moisten (do not soak) a cloth with alcohol and wipe the back half of the rail. After several minutes, wipe the rail with a cloth moistened (not soaked) with deionized water. Wipe with a dry cloth.

4. When the back half of the rail is completely dry, slide the plate carrier transport mechanism to the back of the rail.

5. Repeat step 3 for the front half of the rail.

6. When the front half of the rail is completely dry, replace the microplate carrier and turn on the instrument.

Clean/Replace the Check Valves

Note: The check valves for autoclavable dispensers do not twist open.

If the check valves leak or become stuck, you can either clean or replace them. To order replacment check valves, contact BioTek Customer Service.

To clean a check valve:

1. Pull the tubing off the check valve and pull the valve off the syringe pump port. See Figure 3 in Chapter 2.

2. Insert the stylus into the feed end of the valve to hold it open (observe arrow on valve indicating flow direction).

3. Flush with hot water. If necessary, for the non-autoclavable model, the check valve body twists open to allow disassembly and cleaning of internal components. Note proper orientation for reassembly.

4. Replace the valve and the tubing.



After replacing a check valve, we recommend that you recalibrate the backlash (see below) to ensure optimum accuracy performance.

Calibrating the Backlash

Equipment Required • A minimum 3-place precision balance (all data from 0 to 99 grams)

• Supply bottle

• Priming plate

• Supply Tubing Sets:

For AF1000 Dispensers (PN 7140505)

For AF1000A Autoclavable Dispensers, use PN 7140510, or use the Organic Solvent Tubing Set, PN 7140514 .

Setup 1. Connect the dispenser to a supply bottle that is half-filled with deionized

water. The deionized water container must be at the same level as the dispenser. The liquid level should be 3” (102 mm) from the bench top. While calibrating and testing, maintain the liquid level in the supply bottle at half the 3-inch column height, but start with more fluid to allow for priming.

2. Attach the fitting of the long tube to the supply bottle. Push the opposite end of this tubing onto the bottom port of the syringe pump (see Figure 3 in Chapter 2). The arrow of the check valve must point toward the syringe pump.

Note: For AF1000 dispensers, the check valve will be closer to the syringe pump than to the manifold. For AF1000A dispensers, the check valve will be closer to the manifold.

3. Push one end of the short supply tube onto the top port of the syringe pump. Push the other end over the barbed fitting on the manifold.

Select the Manifold (AF1000A Dispensers Only) Before setting the backlash, it is necessary to select the manifold being tested. Follow the menu path shown below:

UTIL > SETUP > MAN > 16ch or 8ch-96 or 8ch-24 > MAIN MENU KEY



Create a Dispense Program The backlash is adjusted for a 20 μl dispense volume. Create a program that dispenses 20 μl per well at dispense rate 2. Follow the menu path shown below:

DEFINE > CREATE > DISP > key in a program name > set the DISPENSE VOLUME to 0020 and the FLOW RATE to 2 > MAIN MENU KEY > YES

Prime the Plate 1. Place the microplate adaptor into the carrier, and place the priming plate into

the adaptor.

2. Follow the two menu paths shown below:

RUN > PRIME > OPTIONS key until New_Buffer_Prime is displayed > ENTER KEY to select the program > ENTER KEY to start the program > When the prime is complete, press the REPEAT soft key and prime again > MAIN MENU KEY

RUN > DISP > OPTIONS key until the newly created Dispense program is displayed > ENTER KEY to start this program > ENTER KEY to select the defaults for the first strip to dispense and the last strip to dispense > ENTER KEY again to start the program

The MicroFill™ will dispense 20 μl per tube into the priming trough.

3. Empty the Priming Plate as needed.

Calibrate the Backlash 1. Empty/dry the priming plate, place it on a three-place balance, and then tare

the balance.

2. Place the priming plate onto the adaptor and run the 20 μl Dispense program created above.

3. When the program is complete, carefully remove and reweigh the plate, to determine the Actual Weight.

The Expected Weight is:

• 7.680 g for 16-channel manifolds

• 1.920 g for 8-channel manifolds (AF1000A versions only)



4. Calculate the volume (weight) error and the backlash adjustment that needs to be made.

• Subtract the Actual Weight from the Expected Weight and divide by the number of columns dispensed.

• Divide the result by 0.0033 for AF1000A instruments, or 0.0031 for AF1000 instruments, and round to the nearest whole number.

Example: 7.680 - 7.550 = 0.130, 0.130/24 = 0.00542, 0.00542/0.0033 = 1.64 rounded to 2. The backlash setting needs to be adjusted by 2.

Example: 1.920 - 2.010 = -0.09, -0.09/12 = - 0.0075, -0.0075/0.0033 = -2.27 rounded to -2. The backlash setting needs to be adjusted by -2.

5. Adjust the backlash as necessary:

UTIL TESTS press the SHIFT key while simultaneously pressing the HIDDEN KEY #2 (space between the Main Menu and Previous Screen keys) SYR BKLSH enter the new number ENTER KEY MAIN MENU KEY

6. Repeat steps 1 through 5 until the volume dispensed is within one backlash unit of being exact:

• AF1000 dispenser: ± 0.112 ml

• AF1000A dispenser w/16-channel manifold: ± 0.119 ml

• AF1000A dispenser w/8-channel manifold: ± 0.059 ml



Autoclaving the Syringe Head

This section applies to autoclavable versions of the MicroFill™ only.

Note: Refer to Figure 2 in Chapter 2 when performing steps 1-3.

1. Use the supplied 3/32” (2.39 mm) hex wrench to remove the two mounting screws that hold the syringe head to the rear of the instrument.

2. Pull the syringe head straight back and off of the piston.

3. Use the hex wrench to loosen the setscrew and then remove the piston.

Important! The piston and syringe head must be autoclaved separately from one another (see Figure 10 below). Ensure that the piston is removed from the syringe head before autoclaving. If a mistake is made and the piston is set in the syringe head through the autoclave cycle, remove the piston from the syringe head and run the piston (or both piston and syringe head) through the autoclave again.

4. Autoclave at 134°C and 216 kPa for 3 minutes, or 121°C and 115 kPa for 30 minutes. The manifold, tubing, check valves, and supply bottles may also be sterilized in the autoclave.

5. Replace the components as described in Chapter 2.

Figure 10: Autoclave the piston (left) and syringe head (right) separately

Long-Term Storage Maintenance | 57


Long-Term Storage Maintenance

Before leaving the MicroFill™ unused for a long period of time, run the M_Long_Shutdown Maintenance program. This program performs three steps:

• Flushes and soaks the supply tubing and manifold with disinfectant

• Flushes the system with rinse

• Purges the system of fluid Prepare one supply bottle with at least 100 ml of disinfectant (70% isopropanol) and one with at least 100 ml of rinse (deionized water). You also need one empty supply bottle (this is the “air” bottle).

To run the M_Long_Shutdown program, follow the menu path shown below.

MAINT > press the Option key until M_Long_Shutdown appears > CONNECT DISINFECTANT AND PRESS <START> KEY > MAINTENANCE RUNNING PRESS <STOP> TO QUIT

Note: While this program is running, you will need to periodically check the display and follow the instructions for changing the supply bottles.

Storing the MicroFill Dispenser

1. Perform the Long-Term Storage Maintenance as described above.

2. Disconnect the dispenser from its power source.

3. Store the dispenser in a dust- and particle-free environment as described in Chapter 1.

4. Protect the dispenser from temperature extremes that can cause condensation within the unit and from corrosive fumes and vapors.



Decontamination

Purpose

Any laboratory instrument that has been used for research or clinical analysis is considered a biohazard and requires decontamination prior to handling.

Decontamination minimizes the risk to all who come into contact with the instrument during shipping, handling, and servicing. Decontamination is required by the U.S. Department of Transportation regulations.

Persons performing the decontamination process must be familiar with the basic setup and operation of the instrument.

Important! BioTek Instruments, Inc. recommends the use of the following decontamination solutions and methods based on our knowledge of the instrument and recommendations of the Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the Biohazard(s) they handle.

Wear prophylactic gloves when handling contaminated instruments. Gloved hands should be considered contaminated at all times; keep gloved hands away from eyes, mouth, nose, and ears. Eating and drinking while decontaminating instruments is not advised.

Mucous membranes are considered prime entry routes for infectious agents. Wear eye protection and a surgical mask when there is a possibility of aerosol contamination. Intact skin is generally considered an effective barrier against infectious organisms; however, small abrasions and cuts may not always be visible. Wear protective gloves when performing the decontamination procedure.

Decontamination | 59


Tools and Supplies

• 0.5% sodium hypochlorite (NaClO, or bleach) • Surgical mask • 70% isopropyl alcohol (as an alternative to bleach) • Protective gloves • Deionized or distilled water • Lab coat • Priming plate • Biohazard trash bags • Safety glasses • Clean cotton cloths

Decontamination Procedure

Warning! Internal Voltage. Always turn off and unplug the instrument for all decontamination and cleaning operations.

The bleach solution is caustic; wear gloves and eye protection when handling this solution.

Do not immerse the instrument, spray it with liquid, or use a “wet” cloth. Do not allow the cleaning solution to run into the interior of the instrument. If this happens, contact the BioTek Service Department.

Do not soak the keypad. This will cause damage. Wipe the keypad with a damp cloth.

1. Turn off and unplug the instrument.

2. Prepare an aqueous solution of 0.5% sodium hypochlorite (bleach). As an alternative, 70% isopropyl alcohol may be used if the effects of bleach are a concern.

Note: Be sure to check the % NaClO of the bleach you are using; this information is printed on the side of the bottle. Commercial bleach is typically 10% NaClO; if this is the case, use a 1:20 dilution. Household bleach is typically 5% NaClO; if this is the case use a 1:10 dilution.

Isopropyl alcohol is not recommended for removing proteins (such as bovine serum albumin).

3. Remove the dispenser’s mist shield.



4. Moisten a cloth with the bleach solution or alcohol. Do not soak the cloth.

5. Wipe the inside of the mist shield, the plate carrier, microplate adaptor, priming trough, priming plate, and the top surface of the base.

6. Wait 20 minutes. Moisten a cloth with DI or distilled water and wipe all surfaces of the instrument that have been cleaned with the bleach solution or alcohol.

7. Use a clean, dry cloth to dry all wet surfaces.

8. Place a microplate adaptor into the carrier, and place the priming plate into the adaptor. Ensure that the partition dividing the trough is placed toward the rear of the instrument.

9. Run the M_Decontaminate program to decontaminate the internal tubing and manifold parts. Follow the menu path shown below:

MAINT > M_Decontaminate > CONNECT DISINFECTANT AND PRESS <START> KEY RUNNING: DECONTAMINATION/DISINFECTING CONNECT RINSE BOTTLE AND PRESS <START> KEY RUNNING: DECONTAMINATION/RINSING

Note: While this program is running, you will need to periodically check the display and follow the instructions for changing the supply bottles.

10. Reassemble the dispenser as necessary.

11. Discard the used gloves and cloths using a Biohazard trash bag and an approved Biohazard container.

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Chapter 5

Instrument Qualification

Testing is conducted on every MicroFill™ before it leaves BioTek, to verify dispense accuracy and precision.

Note: Disable the AutoPrime feature before conducting the tests.

IQ/OQ/PQ ........................................................................... 62 Recommended Qualification Schedule ..................................... 63 System Self-Check and Checksum Test ................................... 64

System Self-Check ........................................................... 64 Checksum Test ................................................................ 64

Dispense Precision and Accuracy Tests .................................... 64 Overview ........................................................................ 64 Specifications .................................................................. 65 Materials ........................................................................ 66 Test Solutions ................................................................. 67 Dispense Precision & Accuracy Test: 16-Channel Manifold ...... 68 Dispense Precision & Accuracy Test: “8-96” Manifold ............ 70 Dispense Precision & Accuracy Test: “8-24” Manifold ............ 72 Documenting Test Results ................................................. 74 Liquid Test Worksheets Performance Verification Logs

62 | Chapter 5: Instrument Qualification


IQ/OQ/PQ

Installation Qualification (IQ) confirms that the dispenser and its components have been supplied as ordered and ensures that they are assembled and configured properly for your lab environment.

• The recommended IQ procedure consists of setting up the instrument as described in Chapter 2, Installation and then performing the System Self-Check and the Checksum Test.

• The IQ procedure should be performed initially (before the dispenser is used for the first time).

• The successful completion of the IQ procedure verifies that the instrument is installed correctly. The Operational Qualification procedure (see below) should be performed immediately following the successful IQ.

Operational Qualification (OQ) confirms that the dispenser operates according to specification initially and over time.

• The recommended OQ procedure consists of performing the System Self-Check, the Checksum Test, and the appropriate Dispense Precision and Accuracy Tests.

• The OQ procedure should be performed initially (before first use) and then routinely; the recommended interval is annually. It should also be performed after any major repair or upgrade to the hardware or software.

• Although out-of-tolerance failures will be detected by the OQ tests, results should be compared with those from the monthly Performance Qualification tests and previous OQ tests to monitor for trends.

• The successful completion of the OQ procedure, in combination with results that are comparable to previous PQ and OQ tests, confirms that the dispenser is performing consistently over time.

Recommended Qualification Schedule | 63


Performance Qualification (PQ) confirms that the dispenser consistently meets the requirements of the tests performed at your laboratory.

• The recommended PQ procedure consists of performing the System Self-Check, the Checksum Test, and the Dispense Precision and Accuracy Test.

• Your facility’s operating policies may also require that you routinely perform an actual assay, to confirm that the dispenser will consistently give adequate results for the assays to be run with it.

• These tests should be performed routinely; the recommended interval is monthly. This frequency may be adjusted depending on the trends observed over time.

• The successful completion of the PQ procedure confirms that the dispenser is performing consistently under normal operating conditions.

Recommended Qualification Schedule

Task/Test IQ OQ PQ

Initially Initially & Annually Monthly

Installation, Setup, & Configuration

System Self-Check

Checksum Test

Dispense Precision and Accuracy Test



System Self-Check and Checksum Test

System Self-Check

Each time the MicroFill™ is turned on, the System Self-Check is automatically performed to ensure proper instrument operation.

If the self-check passes, the Main Menu will appear and the dispenser is ready for use. If there is a failure, the dispenser will “chirp” and display an error code. Press the Stop key and then look up the error code in Appendix A. If the dispenser fails the self-check, it will not perform a program operation until the error condition is cleared.

The System Self-Check can also be performed manually, by selecting UTIL > TESTS > SLFCHK.

Checksum Test

If the Checksum Test is performed, the initial display will show the on-board (basecode) software part number, version number, and checksum. The second display shows the configuration software information. The Main Menu reappears at the conclusion of the test.

To run the Checksum Test, start at the Main Menu and select UTIL > TESTS > CHKSUM.

Dispense Precision and Accuracy Tests

Overview

Note: Disable the AutoPrime feature before conducting these tests.

Dispense precision is a measure of the variability of volumes dispensed from tube to tube across the manifold. The optical density of the solution in a well is proportional to the total volume of the solution in the well. If the % Coefficient of Variation (%CV) is calculated, the result is a measure of the uniformity of the distribution of dispensed volumes across the manifold. It is the ratio, expressed in percent, of the standard deviation of the distribution of fluid volumes in the wells to the mean value of volume per well. The uniformity of distribution across the manifold improves as the %CV is lowered.

Dispense accuracy is a measure of the average volume dispensed per well. It is independent of precision. The volume per well may vary greatly over a plate, yet the accuracy may be exact because it is an average of the volumes.

Dispense Precision and Accuracy Tests | 65


Specifications

Precision

Note: The specifications listed here are for typical performance. For IQ/PQ/OQ testing purposes, add 1.0% additional tolerance to the %CV to accommodate various test solutions, off-peak wavelengths, reader errors, and pipette errors.

Typical 384-well dispense precision is:

• < 2 %CV when dispensing 80 μl/well

• < 5 %CV when dispensing 20 μl/well Typical 96-well dispense precision is:

• < 2 %CV when dispensing 160 μl/well using the 16-channel manifold

• < 5 %CV when dispensing 40 μl/well using the 16-channel manifold

• < 2 %CV when dispensing 80 μl/well using the 8-96 manifold

• < 5 %CV when dispensing 20 μl/well using the 8-96 manifold Typical 24-well dispense precision is:



Accuracy Typical 384-well dispense accuracy is:

• ± 1.25% when dispensing 80 μl/well

• ± 5% when dispensing 20 μl/well Typical 96-well dispense accuracy is:

• ± 1.25% when dispensing 160 μl/well using the 16-channel manifold

• ± 5% when dispensing 40 μl/well using the 16-channel manifold

• ± 1.25% when dispensing 80 μl/well using the 8-96 manifold

• ± 10% when dispensing 20 μl/well using the 8-96 manifold Typical 24-well dispense accuracy is:

• ± 1.25% when dispensing 160 μl/well using the 8-24 manifold

• ± 10% when dispensing 40 μl/well using the 8-24 manifold



Materials

• Corning Costar #3590 96-well plates, or equivalent (16-channel and “8-96” manifold testing)

• 384-well plates (16-channel manifold testing)

• 24-well plates (“8-24” manifold testing)

• Precision balance with capacity of 100 g minimum and readability of 0.01 g resolution

• Pipettes and graduated beakers

• Microplate absorbance reader capable of dual wavelength reading at 630 and 405 (or 450) nm

If you are using one of BioTek’s keypad-based absorbance readers, such as the ELx800™ or ELx808™, ensure that the reader is not running in Rapid mode. To check this, select UTIL > READ and cycle through the prompts until you see READ IN RAPID MODE?”

• The test solutions described on the following page. See also the important notes below:

The absorbance of blue dye solutions should be measured at 630/450 (or 405) nm. The BioTek blue dye solution part number is 7773001.

The absorbance of yellow dye solutions should be measured at 450 (or 405)/630 nm. The BioTek yellow dye solution part number is 7120782. Note: This solution cannot be used when testing an 8-24 manifold.

The final absorbance for all dye solution concentrations should be in a range between 0.700 and 1.300 OD.

The factory determined the pass/fail specifications for the following tests using the test solutions described. You may choose to use your own solutions. If any tests fail using your own solutions, however, retry the tests using the suggested solutions.

Using pure DI water in place of these solutions is not recommended and will likely result in the failure of the unit to meet specifications.

Liquid Test Worksheet templates can be found at the end of this chapter for recording data reduction results. If your tests are failing, this information will be useful for BioTek TAC to help diagnose any problems.



Test Solutions

For 16-Channel Manifold Testing: 20 µl Solution

• If using BioTek’s blue dye solution, mix 100 ml of DI H2O with 10 ml of dye solution to create a dilution of the concentrate. Mix 160 ml of DI H2O with 20 ml of the diluted concentrate.

• If using BioTek’s yellow dye solution, mix 940 ml of DI H2O with 60 ml of dye solution.

• If using FD&C #5 yellow dye powder, mix 1 liter of DI H2O with 0.105 grams of dye powder.

80 µl Solution

• Mix 120 ml of DI H2O with 40 ml of the 20 μl solution.

For “8-96” Manifold Testing: 20 µl Solution

• If using BioTek’s blue dye solution, mix 100 ml of DI H2O with 10 ml of dye solution to create a dilution of the concentrate. Then mix 140 ml of DI H2O with 80 ml of the diluted concentrate.

• If using BioTek’s yellow dye solution, mix 400 ml of DI H2O with 100 ml of dye solution.


80 µl Solution


For “8-24” Manifold Testing: 40 µl Solution

• If using BioTek’s blue dye solution, mix 140 ml of DI H2O with 12 ml of dye solution.


• Note: BioTek’s yellow dye solution is not concentrated enough to use for this test.

160 µl Solution




Dispense Precision & Accuracy Test: 16-Channel Manifold

Perform the following steps a total of four times to test two plate types (96 and 384), each with two dye solutions.

1. Edit the Dispense program Accuracy_Test as follows (set FLOW RATE to 2 for all tests):

Test 1: PLATE TYPE = 96, VOLUME = 40 μl/well




2. Place the priming plate onto the microplate adaptor of the MicroFill™.

3. Prime the MicroFill™ two times with the test solution.

Tests 1 and 2: Use the 20 μl solution

Tests 3 and 4: Use the 80 μl solution

This will pump any residual air out of the check valves. Remove and empty the priming plate.

4. Place a clean, empty (96- or 384-well) microplate on the balance and then tare the balance.

5. Place the microplate on the MicroFill™ and run the Accuracy_Test program modified in step 1.

6. Place the plate on the balance and record the Total Dispense Weight. This value will be used to calculate the % Accuracy Error in step 11.

7. Using a calibrated hand pipette, dispense deionized water on top of the dye solution in the wells. (Tests 1 and 2 only.)

Test 1: Pipette 120 μl/well (resulting in 160 μl/well)


8. Shake the plate on an orbital shaker or in a microplate reader for 15 seconds, or lightly tap the side of the plate with your finger to agitate the contents of the wells. This will result in a uniformly mixed solution and create a more uniform meniscus in each of the wells.

If you are using one of BioTek’s keypad-based absorbance readers, such as the ELx800™ or ELx808™, ensure that the reader is not running in Rapid mode. To check this, select UTIL > READ and cycle through the prompts.

9. Read the plate in an absorbance reader using the dual-wavelength method, to reduce the influence of scratches and foreign particles that could be in the well. See the recommended wavelengths on page 66. Print or export the results.



For steps 10 and 11, enter the results in copies of the Liquid Test Worksheets at the end of this chapter.

10. Calculate and report the Mean absorbance, Standard Deviation, and the %CV for the wells under test. %CV = (Standard Deviation ÷ Mean) * 100.

11. The % Accuracy Error calculation is:

(Actual Weight – Expected Weight) ÷ Expected Weight x 100

Subtract the expected dispense weight (see below) from the Actual (Total) Dispense Weight (from step 6), and divide the result by the expected weight. Multiply the result by 100.

Example for 96-well plate, 40 μl test, Total Dispense Weight = 3.73 g: (3.73 – 3.84) ÷ 3.84 x 100 = -2.87% = PASS (must be < ±5.0%)

The Expected Dispense Weight is the volume dispensed per well in ml multiplied by the number of wells dispensed. For example, if 40 μl is dispensed to 96 wells, the expected weight is 0.040 x 96 = 3.84 grams. We have calculated some expected dispense weights for you:

Test 1 (96 wells, 40 μl/well): 3.84 grams




12. Analyze your test results. The following is the Pass criteria for each test:

Test 1: %CV < 6.0%* % Accuracy Error < ± 5.0%




* The specifications listed on page 65 are for typical performance. For IQ/PQ/OQ purposes, we have added 1.0% additional tolerance to the %CV to accommodate various test solutions, off-peak wavelengths, reader errors, and pipette errors.

If one or more of your tests are failing, clean the dispense tubes with stylus, re-prime the manifold, and repeat the test(s). If your tests continue to fail, contact BioTek’s Technical Assistance Center.



Dispense Precision & Accuracy Test: “8-96” Manifold

Perform the following steps two times, using two different 96-well plates:


Test 1: VOLUME = 20 μl/well


2. Place the priming plate onto the microplate adaptor of the μFill.


Test 1: Use the 20 μl solution



4. Place a clean, empty 96-well microplate on the balance and then tare the balance.



7. Using a calibrated hand pipette, dispense deionized water on top of the dye solution in the wells.








For steps 10 and 11, enter the results in copies of the Liquid Test Worksheets at the end of this section.





Example for 96-well plate, 20 μl test, Total Dispense Weight = 1.85 g: (1.85 – 1.92) ÷ 1.92 x 100 = -3.64% = PASS (must be < ± 10.0%)

The Expected Dispense Weight is the volume dispensed per well in ml multiplied by the number of wells dispensed. For example, if 20 μl is dispensed to 96 wells, the expected weight is 0.020 x 96 = 1.92 grams. We have calculated some expected weights for you:










Dispense Precision & Accuracy Test: “8-24” Manifold

Perform the following steps two times, using two different 24-well plates:




2. Place the priming plate onto the microplate adaptor of the MicroFill™.





4. Place a clean, empty 24-well microplate on the balance and then tare the balance.



7. Using a calibrated hand pipette, dispense deionized water on top of the dye solution in the wells.








For steps 10 and 11, enter the results in the Liquid Test Worksheets at the end of this section.





Example for 24-well plate, 160μl test, Total Dispense Weight = 3.64 g: (3.64 – 3.84) ÷ 3.84 x 100 = -5.21% = PASS (must be < ± 10.0%)

The Expected Dispense Weight is the volume dispensed per well in ml multiplied by the number of wells dispensed. For example, if 40μl is dispensed to 24 wells, the expected weight is 0.040 x 24 = 0.96 grams. We have calculated some expected weights for you:










Documenting Test Results

The following pages contain Dispense Precision & Accuracy Test Worksheets and Performance Verification Logs. We recommend you make copies of the appropriate pages and use them to record your calculations and test results.

• Each Worksheet records calculations and pass/fail test results for an individual test (e.g., “40 µl / 16-Channel Manifold / 96-Well Plate).

• Each Performance Verification Log records all pass/fail test results for a particular manifold type over time.

© 2008, BioTek Instruments, Inc.

MicroFill™ Dispense Precision & Accuracy Test Worksheet 16-Channel Manifold / 40 µl / 96-Well Microplate

40 µl Dispense Precision Test

Standard Deviation (SD):

Mean Absorbance (sum of all wells ÷ 96)

% CV (SD ÷ Mean x 100) %

% CV must be < 6.0% Pass Fail

40 µl Dispense Accuracy Test

Total Dispense (Actual) Weight: grams

Expected Weight: (ml/well x number of wells dispensed) grams

% Accuracy Error: (Actual Weight – Expected Weight) ÷ Expected Weight x 100 %

% Accuracy Error must be < 5.0% Pass Fail

Visual verification that no well varies considerably from the others Pass Fail

MicroFill Serial Number:

Tests Performed By:

Date:

Reviewed/Approved By:

Date:






% CV (SD ÷ Mean x 100) %









Tests Performed By:

Date:


Date:






% CV (SD ÷ Mean x 100) %









Tests Performed By:

Date:


Date:






% CV (SD ÷ Mean x 100) %









Tests Performed By:

Date:


Date:


MicroFill™ Dispense Precision & Accuracy Test Worksheet “8-96” Manifold / 20 µl / 96-Well Microplate




% CV (SD ÷ Mean x 100) %









Tests Performed By:

Date:


Date:






% CV (SD ÷ Mean x 100) %









Tests Performed By:

Date:


Date:






% CV (SD ÷ Mean x 100) %









Tests Performed By:

Date:


Date:






% CV (SD ÷ Mean x 100) %









Tests Performed By:

Date:


Date:

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Chapter 6

Troubleshooting

This chapter lists problems that you may experience with the MicroFill™, suggests possible solutions for these problems, and provides instructions for receiving technical assistance.

Overview ............................................................................ 76 General Usage Guidelines ...................................................... 77

Before Running Any Program ............................................. 77 Preventive Maintenance .................................................... 77 Programming considerations ............................................. 77

Product Support & Service ..................................................... 78 Returning Instruments for Service/Repair ............................ 78 Contacting BioTek for Applications Support .......................... 78

Troubleshooting Charts ......................................................... 79 Dispenser Start-Up .......................................................... 79 Syringe Movement ........................................................... 79 Fluid Delivery .................................................................. 80 Fluid Leakage .................................................................. 81 Microplate Carrier Movement ............................................. 82 Dispense Manifold Movement ............................................. 82

76 | Chapter 6: Troubleshooting


Overview

Every effort has been made to ensure that the MicroFill™ is extremely reliable and easy to use. Nevertheless, you could experience problems with the dispenser hardware, software, and/or accessories. This chapter offers information to help resolve these problems. This chapter is divided into three major sections:

1. General Usage Guidelines. When operating the dispenser, follow these guidelines to ensure optimal performance and to prevent the problems described in the Troubleshooting Charts. See page 77.

2. Technical Support. If the dispenser fails to function properly, try to solve the problem(s) by following the Troubleshooting Charts in this chapter. If the problem persists, contact BioTek’s Technical Assistance Center (TAC). See page 78 for contact information.

3. Troubleshooting Charts. The charts list problems, their possible causes, and possible solutions. The charts are categorized as follows:

• Dispenser Start-Up, page 79

• Syringe Movement, page 79

• Fluid Delivery, page 80

• Fluid Leakage, page 81

• Microplate Carrier Movement, page 82

• Dispense Manifold Movement, page 82

Note: If you are operating the MicroFill™ with BioTek’s BioStack™ Microplate Stacker and encounter problems during plate transfers between the BioStack and the dispenser, or during communication between the two instruments, refer to the Troubleshooting and Error Codes section of your BioStack Operator’s Manual.

General Usage Guidelines | 77


General Usage Guidelines

Before Running Any Program

• Fill the supply bottles with sufficient fluid. Make sure the supply tube is in the liquid.

• The supply tube should extend to the bottom of the bottle and be cut at the end (see drawing to the right) to ensure free flow of liquid at the bottom of the supply bottle.

• Check the external tubing connections for kinks and clogs.

• Make sure the bottles are clean and do not contain any particles or mold.

• If there are large air pockets in the tubing, run a Prime program using 20 ml before running another program.

• After running the Prime program, leave the priming plate in place and run a Dispense program that dispenses 20 μl per well to 24 columns. This will pump out any additional air that is still in the check valves.

• To avoid creating air bubbles every time the supply bottle is filled, make a mark halfway down the bottle and refill when the fluid level has dropped to that point. Unscrew the cap and let it hang over the side just enough to avoid emptying the inside tube and enough to refill the bottle.

• When placing a microplate into the plate adaptor, make sure that well A1 is in the right rear corner as you face the front of the dispenser. Make sure that the plate is firmly seated in the adaptor and that the adaptor is firmly seated in the carrier.

Preventive Maintenance

Perform Daily/Overnight/Monthly maintenance as described in Chapter 4. Preventive Maintenance includes:

• Cleaning the dispense manifold tubes and chambers

• Cleaning the plate adaptor and carrier

• Cleaning the supply bottles

• Rinsing and checking the tubing and the check valves

Programming considerations

• Ensure that the correct number of strips is specified in Dispense programs. See “Define Dispense Program Options” in Chapter 3.



Product Support & Service

A superior support staff backs all of BioTek’s products. If your instrument or software ever fails to function perfectly, if you have questions about how to use or maintain them, or if you need to send an instrument to BioTek for service or repair, please contact our Technical Assistance Center (TAC).

Our Technical Assistance Center is open from 8:30 AM to 5:30 PM (EST), Monday through Friday, excluding standard U.S. holidays. You can send a fax or an e-mail any time.

Phone: (800) 242-4685 or (802) 655-4740 Fax: (802) 654-0638


You can also request technical assistance from our website: www.biotek.com

Please be prepared to provide the following information:

• Your name and company information, along with a daytime phone or fax number, and/or an e-mail address

• The product name, model, and serial number

• The software configuration information. To see this information, start at the Main Menu and select UTIL > TESTS > CHKSUM.

• For troubleshooting assistance or instruments needing repair, the specific steps that produce your problem and any error codes displayed on the screen.

Returning Instruments for Service/Repair If you need to return an instrument to BioTek for service or repair, please contact the TAC for a Return Materials Authorization (RMA) number and the shipping address. Repackage the instrument properly (see Chapter 2, Installation), write the RMA number on the shipping box, and ship the address provided.

Contacting BioTek for Applications Support BioTek’s fully equipped Application Laboratory provides our on-staff scientists with the means to assist you with the integration of our instrumentation and software with your unique scientific applications.

Phone: (888) 451-5171 E-Mail: [email protected]

Troubleshooting Charts | 79


Troubleshooting Charts

Dispenser Start-Up

Problem Possible Cause Possible Solution

Display (LCD) not on.

Power cord not plugged in. Check power connection. Power inlet pin pushed together. Gently split power inlet pin apart.

Syringe, carrier, or manifold position error.

Syringe, manifold, or carrier is being obstructed. Remove obstruction.

Motor, sensor, or electrical problem.

Turn Dispenser off, wait at least 15 seconds, then turn back on. If Dispenser does not pass its self-test, contact BioTek TAC.

Misaligned carrier or manifold. Contact BioTek TAC.

Syringe piston not seated all the way to the bottom before fastening set screw (autoclavable only).

Reinstall the syringe head (see Figure 2 in Chapter 2).

Carrier or carrier rail dirty. Clean carrier and/or rail.

Syringe Movement


Syringe position error.

Syringe movement is blocked.

Check underneath the dispenser to see if something is interfering with the movement of the syringe. Ensure that the syringe power cable is connected to the PCB. Contact BioTek TAC.

Syringe piston not seated all the way to the bottom before being “setscrewed” into place (autoclavable model only).

Reinstall the syringe piston and syringe head (see Figure 2 in Chapter 2).



Fluid Delivery


Unable to dispense fluid.

Inlet tube not connected at manifold or at bottle.

Check all tubing (see “Tubes and Bottles” in Chapter 2).

Supply tube inside the supply bottle is kinked or disconnected.

Straighten or connect supply tube (see Figure 5 in Chapter 2). Make sure the end is cut as shown on page 77.

Clogged dispense tubes on the Dispenser manifold.

Remove and clean the manifold (see “Clean the Manifold” in Chapter 4).

Inlet tube is not connected to the bottom port of the syringe.

Connect the inlet tube to the lower port of the syringe pump (see Figure 3 in Chapter 2).

Outlet tube is not connected to the top port of the syringe.

Connect the outlet tube to the top port of the syringe pump (see Figure 3 in Chapter 2).

Check valve flow direction is incorrect.

Compare the flow direction of the check valves with Figure 3 in Chapter 2. Switch or replace if necessary.

Check valves are stuck closed.

Clean check valves (see “Clean/ Replace the Check Valves” in Chapter 4).

No fluid. Fill bottles with appropriate fluid. System not primed. Run a Prime program using 20 ml. Faulty syringe pump. Contact BioTek TAC. Set screw not tightened on the syringe pump piston (autoclavable model only).


Plate overfills (floods).

Dispense height too high. Change the Dispense Height in the program (see “Define Dispense Program Options” in Chapter 3).

Volume too large for the vessel. Program a smaller volume.

Dispense rate too fast for volume selected.

Use slower dispense rate or lower volume (see “Define Dispense Program Options” in Chapter 3).

Continued on the next page.

Troubleshooting Charts | 81


(Fluid Delivery, continued)


Uneven dispensing of fluid; wells not filled.

Clogged dispense tubes on the Dispenser manifold.

Remove and clean the manifold (see “Clean the Manifold” in Chapter 4).

Manifold or tubing not adequately primed (air in fluid lines).

Run a Prime program using 20 ml. Follow this with a dispense program that dispenses 20 μl per well for 24 strips.

Dispense flow rate too low. Select a higher flow rate (see “Define Dispense Program Options” in Chapter 3).

Setscrew not tightened on the syringe pump piston (autoclavable only).


Dripping dispense tubes.

Dispense tubing routed incorrectly.

The supply bottle tube must enter the syringe at the bottom.

Fluid Leakage


Fluid leaking from manifold.

Defective seals.

Replace plugs (see “Clean (“Stylize”) the Dispense Tubes” in Chapter 4). Replace o-ring on Dispenser manifold inlet fitting. Contact BioTek TAC.

Check valves are leaking. Clean or replace check valves (see “Clean/Replace the Check Valves” in Chapter 4).

Fittings to manifold are leaking.

Reconnect/reseat the fittings (see Figure 5 in Chapter 2).

Fluid leaking from underneath the instrument.

Defective syringe cup.

Contact BioTek TAC. Leaking o-ring seal(s) (autoclavable model only). Leaking syringe seal. Defective syringe piston.

Fluid leaking from external tubing connector.

Defective connector. Replace fitting (see Figure 5 in Chapter 2).

Worn tubing. Replace tubing (see Figures 3 and 5 in Chapter 2).



Microplate Carrier Movement


Dispense tubes not entering well correctly.

Microplate not properly seated or strips not level.

Reseat microplate in adaptor, or the adaptor in the carrier, or the strips in holder. Make sure the carrier is clean (see “Clean the Plate Carrier” in Ch. 4).

Manifold not compatible with plate.

“8-24” manifold is not compatible with 96-well plates. Use the “8-96” manifold.

Horizontal Dispense Position does not align the tubes in the wells.

Change the Horizontal Disp Pos value in the program (see “Define Dispense Program Options” in Chapter 3).

Dispense tube(s) bent.

Push the supplied stylus into the tube and then gently attempt to straighten the tube using your fingers. If it remains bent, contact BioTek TAC.

Manifold tilted. Check tubing for twists; add manifold screws (p/n 12174).

Carrier position error.

Carrier movement is blocked. Check for/remove any obstruction.

Plate Clear Height set incorrectly.

Check the Plate Clear Height in the program, reset as required.

Dirty carrier or carrier rail. Clean carrier and/or carrier rail.

Dispense Manifold Movement


Manifold position error.

Manifold movement is blocked.

Check orientation of microplate; A1 should be in the right rear corner of the plate carrier as you face the front of the instrument.

Check for/remove any obstructions. Ensure that the manifold is resting on the manifold support bracket (see Figure 1 in Chapter 2). Contact BioTek TAC.

Appendix A

Error Codes

This appendix lists Error Codes that may appear on the MicroFill™. If an error is displayed, locate it in this Appendix and then review the charts in Chapter 6, Troubleshooting.

Overview ............................................................................ 84 General Errors ..................................................................... 84 BioStack Errors .................................................................... 87 Fatal Errors ......................................................................... 88

84 | Appendix A: Error Codes


Overview

An error code is displayed on the MicroFill’s display as a four-digit identifier. The first character will be 0, 1, or A. Both 0 and 1 indicate a non-critical error, in which case the instrument will respond to keypad input. An A as the first character indicates a more serious error. In this case, turn off the dispenser. Upon restarting, you should be able to enter commands using the keypad.

Note: Error codes prefixed with a “B-“ that are displayed on the MicroFill™during operation with the BioStack™ Microplate Stacking System indicate errors generated by the BioStack, or problems with communication between the two instruments. Refer to the “Troubleshooting and Error Codes” section of your BioStack Operator’s Manual for a list of these types of error codes and their descriptions.

General Errors

General Errors indicate non-fatal conditions that require attention. If an error is displayed, locate it in the table and then refer to the charts in Chapter 6, Troubleshooting. Contact BioTek’s Technical Assistance Center if further instructions are necessary.

Note: For the codes that represent motor errors, the fourth digit of the code represents the affected motor: 0 = manifold motor, 1 = carrier motor, 2 = syringe motor. For example, error code 0201 means that the plate carrier motor could not find its optical sensor.

General Errors | 85


Error Define Error Code Brief Description

ABORT_ERR 0x0100 Current Run function aborted

NO_SENSOR_ERR 0x0200 Manifold lift motor could not find its home optical-sensor

NO_SENSOR_ERR 0x0201 Carrier motor could not find its home optical-sensor

NO_SENSOR_ERR 0x0202 Syringe motor could not find its home optical-sensor

AUTOCAL_JIG_ERR 0x0300 Jig sensor state incorrect while performing Manifold motor Autocal

AUTOCAL_JIG_ERR 0x0301 Jig sensor state incorrect while performing Carrier motor Autocal

MOTOR_VERIFY_ERR 0x0400 Manifold motor failed positional verify with home sensor

MOTOR_VERIFY_ERR 0x0401 Carrier motor failed positional verify with home sensor

MOTOR_VERIFY_ERR 0x0402 Syringe motor failed positional verify with home sensor

UNDEF_PROG_TYPE_ERR 0x0500 Undefined program type specified for Instrument

PROGRAM_NUMBER_ERR 0x0600 Program with given number and type does not exist

PROGRAM_SET_ERR 0x0700 No program space available,100 programs currently stored

PROGRAM_NAME_ERR 0x0800 Program with given name and type does not exist

PROGRAM_MISSING_ERR 0x0900 Prime program required for maintenance program missing

PROGRAM_CHECKSUM_ERR 0x0A00 Program Checksum failure

MANIFOLD_AUTOCAL_ERR 0x0B00 Invalid manifold defined in Instrument configuration data

PROGRAM_CREATED_ONBOARD 0x0C00 Error used for serial control for programs created on board

PROGRAM_LOCKED 0x0D00 Program locked so operation denied

PROGRAM_NONERASABLE 0x0E00 Program non-erasable so delete denied

DISPENSE_VOLUME_ERR 0x0F00 Dispense volume not within specified limits



Error Define Error Code Brief Description

INSTR_CNFG_DATA_ERR 0x1000 Instrument configuration data corrupted

INVALID_SUPPLY_ERR 0x1100 Invalid Supply index specified in program

MOTOR_NOT_HOMED_ERR 0x1300 Manifold motor not at home position

MOTOR_NOT_HOMED_ERR 0x1301 Carrier motor not at home position

MOTOR_NOT_HOMED_ERR 0x1302 Syringe motor not at home position

AUTOCAL_ABORT_ERR 0x1400 Mechanical auto-calibration was aborted

MALLOC_ERR 0x1900 Memory allocation failure

SYR_VOLUME_ERR 0x1A00 Syringe volume requested out of range

DISPENSE_POS_ERR 0x1B00 Carrier offset position is not within defined limits

SYR_FMEA_ERR 0x1C01 Sensor not found at start of syringe aspirate stroke

SYR_FMEA_ERR 0x1C02 Sensor not open at start of dispense stroke

SYR_FMEA_ERR 0x1C03 Syringe aspirate stroke failure

SYR_FMEA_ERR 0x1C04 Sensor verify after last syringe stroke in program failed

SYR_FMEA_ERR 0x1C05 Sensor clear from home position too many steps

SYR_ULPERSTEP_ERR 0x1D00 Syringe μl/step factor is not within specified limits

AUTOCAL_CHECKSUM_ERR 0x1E00 Autocal data corrupted or not currently set

DISP_CAL_DATA_ERR 0x1F00 Dispense cal data checksum error

DISP_CAL_DATA_ERR 0x1F01 Dispense cal data not set

DISP_CAL_DATA_ERR 0x1F02 Dispense cal data element out of range

INVALID_LINK_ERR 0x2400 Link program invalid

BioStack Errors | 87


BioStack Errors

Error codes that may be displayed on the MicroFill™ when communicating with the BioStack™:

Error Define Error Code Description

INVALID_PASSWORD 0x8300 An invalid password was sent during a protected operation.

NO_MATCHING_ITEM 0x8301 The BioStack was requested to send some configuration data to the controlling software, and the BioStack did not know about the requested data.

BAD_CHECKSUM 0x8302 A checksum was generated following a code download, which did not match what was expected.

NOT_IN_DOWNLOAD_MODE 0x8303 The BioStack is in an invalid mode for receiving a message.

NAK_RECEIVED 0x8304 The BioStack didn’t properly acknowledge a message sent to it.

INVALID_RESPONSE 0x8305 Invalid message response received from BioStack.

TIMEOUT 0x8306 The serial communications timed out.



Fatal Errors

Fatal errors indicate conditions that require immediate attention. If a fatal error is displayed, contact BioTek’s Technical Assistance Center for further instructions.

Note: For the codes that represent device errors, the fourth digit of the code represents the affected device: 0 = manifold motor, 1 = carrier motor, 2 = syringe motor, 4 = software timer, 5 = display, 6 = quick flash memory.

Error Error Code Brief Description

TCB_NOT_AVAIL_ERR 0xA100 Task control block not available

Undefined 0xA200 Error is currently undefined

NOT_AVAIL_ERR 0xA300 Manifold motor device not available

NOT_AVAIL_ERR 0xA301 Carrier motor device not available

NOT_AVAIL_ERR 0xA302 Syringe motor device not available


NOT_AVAIL_ERR 0xA304 Timer device not available

NOT_AVAIL_ERR 0xA305 Display device not available

NOT_AVAIL_ERR 0xA306 Configuration flash device not available

CHECKSUM_ERR 0xA400 Failed basecode checksum test on power up

POWER_ERR 0xA500 Logic supply voltage below safe level

QFLASH_TIMEOUT_ERR 0xA600 Configuration flash timed out

QFLASH_ERR 0xA700 Configuration flash read didn’t match write


HEAP_CORRUPTED_ERR 0xA900 Memory heap corrupted

Appendix B

Chemical Compatibility

This Appendix lists the materials used in the autoclavable and standard versions of the MicroFill™, and provides information to assist with determining the compatibility of many dispensed chemicals that will come in contact with the system.

Autoclavable MicroFill ............................................................ 90 Table 1. Material/Where Used List ...................................... 90 Table 2. Chemical Compatibility Ratings .............................. 91

Non-Autoclavable MicroFill ..................................................... 92 Table 3. Material/Where Used List ...................................... 92 Table 4. Chemical Compatibility Ratings .............................. 93

90 | Appendix B: Chemical Compatibility


Autoclavable MicroFill

The following materials are used in the autoclavable version of the MicroFill™. Materials listed as 1-12 in Table 1 are cross-referenced as headings 1-12 in Table 2.

Table 1. Material/Where Used List

# Material Where Used

1 Borosilicate Glass Syringe cylinder, supply bottles

2 Polypropylene Bottle cap and fittings, pre-priming trough fitting, check valves (organic solvent tubing set)

3 Silicone Tubing, manifold o-rings, check valve o-rings (autoclavable tubing set)

4 Ultem (polyetherimide) Check valves (autoclavable tubing set)

5 316 Stainless Steel Dispense tubes, syringe piston, cylinder head and fittings, check valve spring

6 PTFE (polytetrafluoroethylene) Syringe seal

7 ETFE (ethylene tetrafluoroethylene)

Manifold fitting

8 PPS (polyphenilene sulfide) Manifold, manifold plugs

9 EP (ethylene propylene) Check valve o-rings (organic solvent tubing set)

10 CPVC (chlorinated polyvinyl chloride)

Priming trough, pre-priming trough

11 Aluminum Carrier, microplate adaptor

12 Polystyrene Assay plates

Autoclavable MicroFill | 91


Table 2. Chemical Compatibility Ratings

Chemical Material

(1-12 represent materials listed in Table 1)

1 2 3 4 5 6 7 8 9 10 11 12

Isopropyl alcohol 70% A A A A A A A A A B A A

Ethyl alcohol 70% A A B A A A A A A B A A

Methyl alcohol 70% A A A A A A A A A A A ND

Formaldehyde 40% A A B A A A A A A A B ND

Hydrocholoric acid 20% A B D A D A A D A A D C

Hydrogen peroxide 10% A A A A B A A A A A A A

Sodium hydroxide 20% A A A A B A A A A A D A

Sodium hypochlorite <20% A A B B C A A C B A D A

Sodium hypochlorite 0.5% A A B A B A A A B A D A

Sulfuric acid <10% A A C A B A A A B A B A

Ethylene oxide A D D ND B A A D C C D C

Detergents 1% A A A A A A A A A A B A

10% Virkon A A A ND A ND ND ND A A D A

Acetonitrile A A C D A A A A C D B D

DMSO dimethylsulfoxide A A C D A A A A B D A D

Key: A = No effect; B = Slight effect; C = Moderate effect; D = Severe effect; ND = No data

Note: We advise against continuous contact with harsh chemicals. It is best if the dispenser is rinsed with deionized water after contact with any strong acid, base, or solvent.

Please contact BioTek with questions about compatibility of any chemicals not described here.



Non-Autoclavable MicroFill

The following materials are used in the standard version of the MicroFill™. Materials listed as 1-10 in Table 3 are cross-referenced as headings 1-10 in Table 4.

Table 3. Material/Where Used List

# Material Where Used

1 Polyethylene Syringe seal, buffer bottle

2 Polypropylene Fittings in bottles, inline fittings

3 Kynar (polyvinilidinefluoride, PVDF)

Check valves

4 316 SS Dispense tubes, syringe piston, check valve spring

5 Noryl (polyphenylene ether + polystyrene)

Syringe cylinder

6 CPVC (chlorinated polyvinyl chloride)

Manifold, microplate adaptor legs, manifold plugs

7 Aluminum Carrier, microplate reader

8 Polystyrene Assay plates

9 Silicone Tubing, manifold seal o-ring, manifold plug o-rings

10 EP (ethylene propylene) Check valve o-rings

Non-Autoclavable MicroFill | 93


Table 4. Chemical Compatibility Ratings

Chemical Material

(1-10 represent materials listed in Table 3)

1 2 3 4 5 6 7 8 9 10

Isopropyl alcohol 70% B A A A A B A A A A

Ethyl alcohol 70% B A A A A B A A B A

Methyl alcohol 70% A A A A A A A ND A A

Formaldehyde 40% D A A A A A B ND ND A

Hydrochloric acid 20% A B A D A A D C D A

Hydrogen peroxide 10% A A A B A A A A A A

Sodium hydroxide 20% D A A B A A D A A B

Sodium hypochlorite <20% A A A C A A D A B B

Sodium hypochlorite 0.5% A A A B A A D A B B

Sulfuric acid <10% A A A B A A B A C A

Ethylene oxide A D A B A C D C D C

Detergents 1% B A A A A A B A A A

10% Virkon A A ND A ND A D A A A

Acetonitrile A A D A D D B D C C

DMSO dimethyl sulfoxide A A D A A D A D C B

Key: A = No effect; B = Slight effect; C = Moderate effect; D = Severe effect; ND = No data

Note: We advise against continuous contact with harsh chemicals. It is best if the dispenser is rinsed with deionized water after contact with any strong acid, base, or solvent.

Please contact BioTek with questions about compatibility of any chemicals not described here.



Appendix C

Dispense Flow Rates

This Appendix lists valid dispense flow rates throughout the selectable volume range.

Standard (Non-Autoclavable) Versions of the MicroFill™ ............. 96 Autoclavable Versions of the MicroFill ...................................... 97

16-Channel Manifold ........................................................ 97 “8-96” Manifold ............................................................... 97 “8-24” Manifold ............................................................... 97

96 | Appendix C: Dispense Flow Rates


Standard (Non-Autoclavable) Versions of the MicroFill™

Volume Range Plate Type Valid Rate(s)

5 – 1500 μl 384 1

10 – 3000 μl 96 1

20 – 1500 μl 384 1-2

40 – 3000 μl 96 1-2

30 – 1500 μl 384 1-3

60 – 3000 μl 96 1-3

40 – 1500 μl 384 1-4

80 – 3000 μl 96 1-4

45 – 1500 μl 384 1-5

90 – 3000 μl 96 1-5

Autoclavable Versions of the MicroFill | 97


Autoclavable Versions of the MicroFill

16-Channel Manifold


5 – 1500 μl 384 1

10 – 3000 μl 96 1

10 – 1500 μl 384 1-2

20 – 3000 μl 96 1-2

25 – 1500 μl 384 1-3

50 – 3000 μl 96 1-3

30 – 1500 μl 384 1-4

60 – 3000 μl 96 1-4

40 – 1500 μl 384 1-5

80 – 3000 μl 96 1-5

“8-96” Manifold


10 – 3000 μl 96 1-2

20 – 3000 μl 96 1-3

40 – 3000 μl 96 1-4

60 – 3000 μl 96 1-5

“8-24” Manifold


20 – 3000 μl 24 1-2

40 – 3000 μl 24 1-3

80 – 3000 μl 24 1-4

120 – 3000 μl 24 1-5

98 | Appendix C: Dispense Flow Rates


Appendix D

Packing Instructions

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Documents

MicroFill Operator's Manual - Pera Medikalperamed.com/peramed/docs/microfill operator manual.pdf · vi | Preface BioTek Instruments, Inc. Decontamination Procedure ..... 59 Daily/Overnight