Miami Nature BiotechnologyShort Reports, Volume 11

Advances in Gene Technology:

DNA, RNA and CANCER

February 5-9,2000

Proceedings of the 2000

Miami Nature Biotechnology Winter SymposiumPublished by Oxford University Press

Editors

Sandra Black, Paul Boehmer, Kermit L. Carraway,

Murray P. Deutscher, Susan Hassler, Frans Huijing,Richard S. Myers and William J. Whelan

Sponsored by

The University of Miami and Nature Publishing Company

Organized byThe University Biochemistry and Molecular Biology Foundation, Inc.,

The Sylvester Comprehensive Cancer Center and

Nature BiotechnologyUB/TIB Hannover 89

__^__ 120 534 045

CONTENTS

Preface in

Acknowledgments v

GENETIC INSTABILITY

GENETICS OF HUMAN CANCER 3

Carlo M. Croce

ROLE OF THE COMMON FRAGILE SITES IN CANCER DEVELOPMENT 4

David I. Smith, Erik Thorland, Kurt Krummel, Masako Kawakami, Shannon Myersand Lewis Roberts

THE SACCHAROMYCESPiflp AND Rrm3p HELICASES HAVE ANTAGONISTIC 5

EFFECTS ON REPLICATION OF TLOMERIC AND RDBOSOMAL DNA

Virginia A. Zakian

TELOMERES STABILIZE THE GENOME AND ARE THE MOLECULAR 6

DETERMINANTS OF HUMAN CELLULAR AGING

Jerry W. Shay

ETIOLOGY OF CANCER: MISMATCH REPAIR ENZYMES THAT ACT ON 7

MODIFIED BASES IN HUMAN DNA

Josef Jiricny

DNA NUCLEOTIDE EXCISION REPAIR AND CANCER 8

Richard D. Wood

GENETIC CHANGES IN RHABDOMYOSARCOMA INTERFERE WITH p53 9

FUNCTION IN TRANS

Catherine Degnin, Jing Huang and Mathew Thayer

A ROLE FOR NUCLEASE ACTIVITY IN RecBCD RECOMBINATION 10

Maria-Elena Jockovich and Richard Myers

DENATURING HPLC ANALYSIS: ANEW METHOD TO EXAMINE 10

ALLELIC LOSS IN CANCERS

Akira Yokomizo, Donald J. Tindall, Ken Taniguchi, Peter J. Oemer,

David I. Smith and Wanguo Liu

VII

RECONSTITUTED TELOMERASE ACTIVITY OF PURIFIED 11

Htert and Htr IN VITRO

Kenkichi Masutomi, Shuichi Kaneko, Naoyuki Hayashi, Tatsuya Yamashita,Yukihiro Shirota, Ken-ichi Kobayashi and Seishi Murakami

Mtsl (CAPL) EXPRESSION LEAD TO GENOME INSTABILITY 12

A.M. Onischenko and S. Stromblad

LOSS OF MLH1 DUE TO PROMOTER HYPERMETHYLATION IS 12

ASSOCIATED WITH DRUG RESISTANCE, BUT NOT A

METHYLATOR PHENOTYPE, IN OVARIAN CANCER

Gordon Strathdee, Jane Plumb and Robert Brown

CELL CYCLE AND DNA REPLICATION

REGULATING CHROMOSOMAL DNA REPLICATION 15

JohnF.X. Diffley

DIFFERENT ROLES IN DNA REPLICATION FOR DAMAGE-SPECDTC 16

POLYMERASES t\ and £ IN HUMAN XERODERMA PIGMENTOSUM

(XP) CELLS

James E. Cleaver

CONTROL OF DNA REPLICATION IN NORMAL AND NEOPLASTIC CELLS 17

Ronald A. Laskey, Nick Coleman, Dawn Coverley, Torsten Krude, Tony Mills,

Kai Stoeber and Gareth Williams

S. POMBE CELLS LACKING THE CATALYTIC DOMAINS OF DNA 18

POLYMERASE EPSDLON ARE VIABLE, BUT CELL GROWTH IS

DEPENDENT ON THE INTACT MITOTIC CHECKPOINT CONTROL

Wenyi Feng and Gennaro D'Urso

BREAKING CELL CYCLE ARREST IN OOCYTES 18

Joan V. Ruderman, Thorkell Andresson and Laurie Littlepage

BIOSENSORS DESIGNED FOR MEASURING THE 19

POST-TRANSLATIONAL REGULATION OF DNA TOPOISOMERASE H

Liliane Assairi

viii

CYSTEINE 111 AFFECTS COUPLING OF SINGLE-STRAND DNA-BINDING 19

TO ATP HYDROLYSIS TN THE HERPES SIMPLEX VIRUS TYPE-1

UL9 PROTEIN

Deborah A. Sampson, Mercedes E. Arana and Paul E. Boehmer

A NOVEL RAD24 CHECKPOINT PROTEIN COMPLEX CLOSELY 20

RELATED TO REPLICATION FACTOR C

Catherine M. Green, Hediye Erdjument-Bromage, Paul Tempst and Noel F. Lowndes

ENGINEERED ZINC FINGERS THAT BIND TELOMERIC DNA 20

SEQUENCESSachin D. Patel, Mark Islan, Yen Choo and Shankar Balasubramanian

EXPRESSION OF THE HUMAN PCNA GENE IS MODULATED 21

THROUGH SYNTHESIS OF AN ANTISENSE RNA AND S-PHASE

DEPENDENT BINDING OF E2F COMPLEXES IN TNTRON 1

Stella Tommasi and Gerd P. Pfeifer

LFM-1 CHROMOSOMAL-SCAFFOLD PROTEIN. EVIDENCE FOR ITS 21

INVOLVEMENT IN CELL PROLIFERATION

Doral E. Vega-Salas, Manuel Fernandez, III, and Elisa Oltra

GROWTH INHIBITORYEFFECT OF PEPTIDES DERIVED FROM 22

THE C-TERMINAL DOMAIN OF P21WAF IS MEDIATED BY CYCLTN

D1/CDK4INHD3ITION: PCNA BINDING IS NOT ESSENTIAL

Nikolai Z. Zhelev, Daniella I. Zheleva, Peter M. Fischer, Susan V. Duff,

Anna-Lisa Gavine and David P. Lane

GENE EXPRESSION

CHROMATIN REMODELING: MECHANISMS AND REGULATION 25

Zhaohui Shao, Gavin Schnitzler, Said Sif, Michael Phelan, Florian Raible, Ramin

Mollaaghababa, Chao-Ting Wu, Welcome Bender and Robert E. Kingston

DESIGN OF CELL-PERMEABLE PEPTD3ES INHIBITOR OF 25

CDK4/CYCLINDl COMPLEX

Daniella I. Zheleva, Peter M. Fischer, Nikolai Z. Zhelev, Jean E. Melville,

Anna-Lisa Gavine and David P. Lane

IX

STRUCTURE AND COACTTVATOR FUNCTIONS OF THE HUMAN 26

TRAP/SCC/MEDIATOR COMPLEX

Robert G. Roeder, J.D. Fondell, W. Gu, M. Guermah, J. Qin, M. Ito,

S. Malik, S. Yamamura and C.-X. Yuan

REGULATION OF SIALOMUCTN COMPLEX/MUC4 27

EXPRESSION IN MAMMARY EPITHELIAL CELLS

Xiaoyun Zhu, Shari A. Price-Schiavi and Kermit L. Carraway

TRANSLATION CONTROL AND CELL GROWTH 28

Nahum Sonenberg, Brian Raught, Stephanie Pyronnet, Hiroaki Imataka, Shigenobu Morino,

Vitaly Polunovsky, Peter Bitterman and Anne-Claude Gingras

elF5 IS A CORE COMPONENT OF TRANSLATION INITIATION 29

FACTOR ASSEMBLY FORMet-tRNA,Met BINDING TO RTBOSOMESKatsura Asano, Jason Clayton, Lon Phan, Anath Shalev and Alan G. Hinnebusch

RNA ANALYSIS BY ION-PAIR REVERSED-PHASE HPLC 29

Arezou Azarani, Robert Haefele and Karl H. Hecker

USE OF HOUSEKEEPING GENES FOR EQUALIZATION OF SAMPLES 30

IN A NOVEL METHOD FOR THE RAPID ANALYSIS OF GENE

EXPRESSION (RAGE)Indira Carey, Anna M. Smith, Teri Jones-Heiland, Jill M. Ray, Gregory B. Carey,David W. Scott and Michael C. MacLeod

THE SECOND AND THIRD WW DOMAINS OF THE HUMAN NEDD4 31

INTERACT WITH THE CARBOXY TERMINAL DOMAIN OF HUMAN

RNA POLYMERASE H

Alex Chang, Xavier Espanel and Marius Sudol

DOWN REGULATION OF UROGUANYLIN AND GUANYLIN mRNA 32

TRANSCRIPTS IN COLON POLYPS AND ADENOCARCINOMAS

Leonard R. Forte, Kunwar Shailubhai, Sammy L. Eber, Yuan Wang,Brent W. Miedema and Hyun DjuKim

ANOTHER JOB FOR CK2p 32

Barbara Guerra and Olaf-Georg Issinger

REGULATION OF SIALOMUCIN COMPLEX BY TRANSFORMING 33

GROWTH FACTOR p (TGF-p) IN THE RAT UTERUSNebila Idris and Kermit L. Carraway

x

INCREASED EXPRESSION OF SWE1 mRNA SUGGESTS AN 33

ACTIVATION OF A MORPHOGENESIS CHECKPOINT IN

TYPE H MYOSIN DEFICDZNT SACCHAROMYCES CEREVISIAE

Wilson Rios, Margarita Irizarry-Ramirez, Diana Gomez, Felix Rivera,Lilliam Villanueva, Sahily Gonzalez and Jose Rodriguez-Medina

SCREENING AND CLONING OF NOVEL TRANSCRD7TION FACTORS 34

INVOLVED IN BLOOD CELL DEVELOPMENTAND LEUKEMOGENESIS

Ronald G. Nachtman, James M. Abdullah, Jorge L. Infante and Roland Jurecic

ANALYSIS OF STEROID HORMONE RECEPTOR-DNA TRANSCRD?TION 34

COMPLEXES BYELECTROSPRAY MASS SPECTROMETRY

Rajiv Kumar and Stephen Naylor

EAPl/Daxx, ETS1 ASSOCIATE PROTEIN, REPRESSES 35

TRANSCRIPTIONAL ACTIVATION OF ETS1 TARGET GENES

Runzhao Li, Huiping Pei, Dennis K. Watson and Takis S. Papas

CHARACTERIZATION OF SATELLITE 2 TRTMETHYLATION 35

Steven H. Miller and Lloyd M. Epstein

THE HUMAN "DEAD-BOX" PROTEIN p72 INTERACTS WITH THE 36

HIGHLY RELATED PROTEINp68Varrie C. Ogilvie and Frances V. Fuller-Pace

SEQUENCE ANALYSIS OF THE HIES REGION OF HEPATITIS C VHtUS 36

Takayoshi Sasano, Jun-Ichi Sagara, Shugo Nakamura, Mitsunori Dceguchiand Kentaro Shimizu

CRITICAL ANALYSIS OF CDNA FOR GENE EXPRESSION STUDIES 37

Anna M. Smith, Indira Carey, Teri Jones and Jill Ray

REGULATORY SUB-COMPLEX OF PROTEASOME 19S IS INVOLVED 37

IN THE TRANSCRTPTION

Liping Sun, Steven Russell, Anwarul Ferdous, Fernando Gonzalez,

Stephen Johnston and Thomas Kodadek

BIOCHEMICALAND CYTOLOGICAL STUDIES ON TWO 38

DOUBLE-STRANDED RNA BINDING DOMAINS OF THE

DOUBLE-STRANDED RNA ACTIVATED PROTEIN KINASE, PKR

Bin Tian and Michael B. Mathews

XI

ESF, A NOVEL ETS GENE WHOSE EXPRESSION IS INVERSELY 38

CORRELATED WITH CELLULAR TRANSFORMATION

Victor I. Sementchenko, Takis S. Papas and Dennis K. Watson

REQUIREMENT OF STAT3-C-JUN COOPERATION AND SERINE 39

PHOSPHORYLATION FOR MAXIMAL ACTIVATION DRIVEN BY

THE a2-MACROGLOBULTN ENHANCER

Xiaokui Zhang and James E. Darnell

GENE EXPRESSION AND TUMOR CELLS

PROMOTER HYPERMETHYLATION AND GENE SILENCING IN CANCER 43

Stephen B. Baylin and James G. Herman

VISUALIZATION OF ALTERNATIVE EBV EXPRESSION PROGRAMS 44

BY FISH AT CELL LEVEL

Anna Szeles, Kerstin I. Falk, Stephan Imreh and George Klein

A NOVEL CHROMOSOMAL PROTEIN BELONGING TO THE 45

BROMODOMATN SUPERFAMELY REGULATES MULTIPLE STAGES

OF CELL CYCLE

Anup Dey, Tetsuo Maruyama, Jae-Hun Cheong, Farideh Chitsaz, Andrea Farina

and Keiko Ozato

CELL REGULATION THROUGH THE p53 PATHWAY 46

Moshe Oren, Gil Blander, Alexander Damalas, Tanya Gottlieb, Tamar Juven-Gershon,

Juan Fernando Martinez Leal, Ruth Maya, Ohad Shifman, Tamar Unger,Rami Khosravi, Dganit Shkedi, Josef Shilo, Ygal Haupt, Avri Ben-Ze'ev

and Benjamin Geiger

BFGF AS SURVIVAL FACTOR ACTS UPSTREAM AND DOWN STREAM 47

OF CASPASE-3

Chantal Bayer and Christa Cerni

YEAST SACCHAROMYCES CEREVISIAEAS A MODEL ORGANISM FOR 47

UNDERSTANDING THE FUNCTIONS OF THE HUMAN BREAST CANCER

SUPPRESSOR GENE PTEN/TEP1

Ludmilla Berenfeld, Jennifer Collins, Stephen Dove, JoAnne Englebrecht,Jennifer Heymont, Alexandra Kaganovich, Bradford Maynes, Aaron Moulin,

Gregory A. Petsko and Hong Sun

REGULATION OF HER2/NEU EXPRESSION BY ER81 48

Denis G. Bosc and Ralf Janknecht

XII

CHARACTERIZATION OF THE "DEAD-BOX" RNA HELICASE, p68, 48

IN COLON TUMORS

Mirsada Causevic, Neil M. Kernohan, Francis A. Carey and Frances V. Fuller-Pace

C-MYC-MEDIATED TELOMERASEACTIVATION IS NOT ESSENTIAL 49

FORMALIGNANT TRANSFORMATION OF RAT CELLS

Soleman Sasgary, Matthias Wieser and Christa Cerni

SPONTANEOUS MELANOMA DEVELOPMENT IN TRANSGENIC MICE 49

Suzie Chen, Steven M. Crespo-Carbone, Karine Cohen-Solal, Kevin Ryanand Aruna Koganti

RING3, A BROMODOMATN PROTEIN LOCALIZES TO CHROMOSOMES 50

DURING MITOSIS

Farideh Chitsaz, Anup Dey, Andrea Farina and Keiko Ozato

ROLE OF INTERSTITIAL COLLAGENASES IN HUMAN OVARIAN 50

NEOPLASMS

Z. Gunja-Smith, Y. Liu, M. Del Compo, P. Soto and J.F. Woessner, Jr.

REGULATION OF TRANSCRIPTION FACTOR AP-2y EXPRESSION 51

IN BREAST CANCER CELLS

Mark D. Hasleton, Ann Skinner and Helen C. Hurst

HEMIZYGOUS GYRO (Gy/Y) MOUSE FIBROBLASTS, WHICH HAVE A 51

PARTIALLYDELETED SPERMINE SYNTHASE GENE, EXHD3IT

INCREASED RESISTANCE TO OXIDATIVE STRESS

Jonas A. Nilsson, Amel Gritli-Linde, Anders Linde and Olle Heby

ACTIVATION AND REGULATION OF THE HIF-4 TRANSCRTPTION 52

FACTORIN HUMAN T CELL LEUKEMIA VIRUS (HTLV-1)INFECTRED CELLS

John Hiscott, Yael Mamane, Sonia Sharma and Rongtuan Lin

HIGH THROUGHPUT SCREENING OF DIFFERENTIALLY EXPRESSED 52

GENES BY COMBING SUPPRESSION SUBTRACTTVE HYBRIDIZATION

(SSH) AND CDNA EXPRESSION ARRAY TECHNOLOGY

Sejal H. Desai, Sai W. Htun, Luda Diatchenko, Karim Hyder, Jason E. Hill,and Paul D. Siebert

xiii

USE OF SUPPRESSION SUBTRACTTVE HYBRIDIZATION (SSH) AND 53

CDNA EXPRESSION ARRAYS TO IDENTIFY GENES DIFFERENTIALLY

EXPRESSED IN RESPONSE TO CELLULAR TRANSFORMATION BY

ONCOGENES

S. Desai, K. Hyder, L. Diatchenko, S. Htun and P. Seibert

THE ELIMINATION TEST IDENTIFIED A ONE MEGABASE COMMON 53

ELIMINATEDREGION (CER1) AT 3 p 21.3

Ying Yang, Hajnalka Kiss, Maria Kost-Alimova, Anna Szeles, Irina Kholodnyuk,Darek Kedra, George Klein, Jan P. Dumanski and Stephan Imreh

TNHD3ITION OF APOPTOSIS BY OVEREXPRESSION OF SIALOMUCIN 54

COMPLEX (MUC4) IN TRANSFECTED A375 MELANOMA CELLS

Scott Jepson and Kermit L. Carraway

CHARACTERIZATION OF THE EXPRESSION OF THE ZINC 54

METALLOPROTEASE MEPRTN IN PANCREATIC CARCINOMAS

Sarah K. Johnson and Randy S, Huan

COMPARISON OF GENE EXPRESSION PATTERNS IN NEOPLASTIC 55

AND NORMAL HUMAN MAMMARYEPITHELIAL CELLLINES

USING cDNA MICROARRAY

Gregory T. Klus and Zoltan Szallasi

AN ESSAY ON TEN YEARS OF STUDIES OF CTCF: THE UNIQUE 55

CONSERVED TRANSCRIPTION FACTORTHAT PERFORMS MULTIPLE

FUNCTIONAL ROLES BY EMPLOYING MULTDPLE ZINC FINGERS

MEDIATING MULTIPLE DNA SEQUENCE SPECD3TCITY

Victor V. Lobanenkov

BASIC MECHANISMS OF SMC/MUC4 TRANSCRIPTIONAL REGULATION 56

Aymee Perez, Shari A. Price-Schiavi, Roy Barco and Kermit L. Carraway

TELOMERE LENGTH DYNAMICS IN HUMAN SOMATIC CELL HYBRIDS: 56

EVIDENCE FOR A TELOMERE LENGTH FEEDBACKCONTROL

MECHANISM

Kilian Perrem and Roger R. Reddel

NPM/ALK IMMORTALIZES AND IN COOPERATION WITH RAS 57

TRANSFORMS PRIMARY EMBRYONIC CELLS

D. Polgar, P. Duchek, S. Fassl, A. Lamprecht, I. Simonitsch, G. Krupitza and C. Cerni

xiv

EFFECT OF CURCUMIN ON CASPASE 3 AND TELOMERASE ACTIVITY 57

IN HUMAN MAMMARY EPITHELIALAND BREAST CARCINOMA

CELL LINES

Cheppail Ramachandran, Hugo B. Fonseca, Perseus Jhabvala, Steven J. Melnick

and Enrique Escalon

EFFECTS OF SIALOMUCIN COMPLEX (MUC4) ON CELL-CELL 58

ADHESION IN MCF-7 CELLS

D. Ratovondrahona-Ballarin, M. Komatsu and C. Carraway

IDENTDjICATION OF DEFERENTIALLY EXPRESSED GENES IN A 58

MMTV-NEU/S100A4 TRANSGENIC MOUSE MODEL OF METASTATIC

BREAST CANCER

Peter T. Simpson, Roger Burrachlough, Nigel Ualliwell, Philip S. Rudland,Balvinder S. Shoker, D. Ross Sibson and Michael P.A. Davies

DISCRIMINATION OF DRUG SENSITIVITY OF CANCER USING cDNA 59

MICROARRAY AND MULTIVARIATE STATISTICAL ANALYSIS

Tatsuhiko Tsunoda, Yoshiaki Hojo, Chikashi Kihara, Norihiko Shiraishi,Osamu Kitahara, Kenji Ono, Toshihiro Tanaka, Toshihisa Takagi and

Yusuke Nanakmura

PRODUCTION OF SOLUBLE CD44ISOFORMS BY HUMAN CANCER CELLS 59

Jeanine Ward, Qin Yu, Lei Huang and Bryan Toole

IDENTIFICATION OF ACTIN-BINDTNG MOTIFS OF p58, A MEMBRANE- 60

AND MICROFDLAMENT-ASSOCIATED PROTEIN, VIA DELETION

MUTANTS ANALYSIS

Baotong Zhang, Jiaqi Huang, Kermil L. Carraway and Coralie A. Carothers-Carraway

CANCER DIAGNOSTICS

THE APPLICATION OF GENOMICS TO THE DISCOVERY OF CANCER 63

CAUSING GENES

Jeffrey M. Trent

TRANSCRD?TIONAL GENE EXPRESSION PROFILING OF COLORECTAL 64

TUMORS AND CELL LINES: TOWARDS A MOLECULAR TAXONOMY OF

CANCER

Daniel A. Notterman, U. Alon, A.J. Sierk and A.J. Levine

xv

SMART PCR-GENERATED CDNA FOR DEFERENTIAL GENE 65

EXPRESSION STUDIES IN TUMOR AND NORMAL TISSUES

Bakhyt Zhumabayeva, Luda Diatchenko and Paul D. Siebert

BRCA1 AND BRCA2 MUTATION ANALYSIS IN AT-RISK 66

AFRICAN-AMERICAN FAMILD2S: RESULTS AND IMPLICATIONS

Lisa Baumbach, Luis Gayol, Tom Scholl, Hugo Basterrechea, Ingrid Pfeifer,Jennifer Davies, Erasmo Perera, Selina Smith and J. Fernando Arena

MICROSATELLITE INSTABDLITY IN GASTRIC CARCINOMAS FROM 66

SLOVENIAN PATIENTS USING AUTOMATED FLUORESCENT

PCR - MICROSATELLITE ANALYSIS

Barbara Gazvoda, Stojan Repse, Robert Juvan and Radovan Komel

ELECTRONIC ACCESS TO DATA FROM MOUSE CANCER MODELS: 67

THE MOUSE TUMOR BIOLOGY (MTB) DATABASE

Debra M. Krupke, Carol J. Bult, John P. Sundberg and Janan T. Eppig

PTEN/MMAC1 MUTATIONS CORRELATE INVERSELY WITH AN 67

ALTEREDp53 GENE IN GYNECOLOGICAL TUMORS

Deborah Lyn, Thomas Murphy, Nicole A. Bennett, Ruey S. Lin,

Chang-Yao Hsieh, Emmanuel Soyoola and Roland Pattillo

IDENTIFICATION OF FRIZZLED-LDXE CYSTEINE RICH DOMAINS IN THE 68

EXTRACELLULAR REGIONS OF TYROSINE KINASES: POTENTIAL FOR

ANTIBODYMEDIATED THERAPEUTICS

Daruka Mahadevan, Jus Singh and Jose Saldanha

PROSTATE SPECIFIC MEMBRANE ANTIGEN (PSMA) AND TRANSFERRIN 68

RECEPTOR (TfR) HAVE AN AMINOPEPTIDASE DOMAIN: IMPLICATIONS

FOR DRUGDESIGN

Daruka Mahadevan, Jus Singh and Jose Saldanha

MUC4 (SIALOMUCIN COMPLEX) EXPRESSION SQUAMOUS CELL 69

CARCINOMA OF THE UPPERAERODIGESTTVE TRACT

Donald T. Weed, Carmen Gomez-Fernandez, Esteban Bonfante, Thomas D. Lee,

Jeffrey Pacheco, Maria E. Carvajal, W. Jarrard Goodwin, Kermit L. Carraway

xvi

CANCER THERAPEUTICS

OLIGONUCLEOTIDE-BASED APPROACHES FOR THE TREATMENT 73

OF CANCER

C. Frank Bennett

CONSTRUCTING CANCER THERAPEUTICS FROM PROTEINS 74

Richard J. Youle

LOCAL PRODRUG-INDUCED GENE THERAPY OF PANCREATIC 75

CANCER: INTRA-ARTERIAL APPLICATION OF CYTCOCHROME P450

EXPRESSING ENCAPSULATED CELLS: APHASE I/H CLINICAL TRIAL

Walter H. Gunzburg, J.M. Lohr, P. Karle, J. Hain, H. Bergmeister, W. Henninger,A. Hoffmeyer, J-C. Kroger, U. Losert, P. Muller, A. Probst, M. Renner, R.M. Sailer

and B. Salmons

MOLECULAR GENETIC INTERVENTIONS FOR CANCER 76

Gary J. Nabel

REPLICATION BYPASS OF CISPLATIN LESIONS 77

Nicolas Tanguy Le Gac, Giuseppe Villani and Paul E. Boehmer

CISPLATIN - FROM DNA DAMAGE TO CANCER THERAPY 78

Stephen J. Lippard

CELLULAR DELIVERY OF PNA VIA DENOVO DESIGNED TEMPLATE 79

ASSEMBLED PORATING PEPTIDES (MOLECULAR TAPP):TARGETING TELOMERASE?

Eric T. Grumpier, Ganesh Venkataraman and Robert Langer

A NOVEL ANTITUMOR DRUG CMT-3 ALTERS MITOCHONDRIAL 80

PERMEABILITY AND ACTIVATES CASPASES IN PROSTATE

TUMOR CELLS

Eva Escatel, Baoqian Zhu and Bal L. Lokeshwar

LABELING PNA THROUGH ITS C-TERMINUS AND INHIBITION 80

OF HUMAN TELOMERASE BY PNA-PEPTIDE CONJUGATES

Xiaohai Liu and Shankar Balasubramanian

xvii

COMBINED CHEMOTHERAPY OF MURINE MAMMARY TUMORS 81

BY LOCAL ACTIVATION OF THE PRODRUGS D70SFAMIDE AND

5-FLUOROCYTOSINE

Brian Salmons, Wolfgang Gelbmann, Thomas Kammertons, Peter Karle,Robert Sailer, Matthias Renner, Wolfgang Uckert and Walter H. Gunzburg

POSTOPERATIVE IMMUNO-GENE THERAPY OF MURINE 81

BLADDER TUMOR BYIN VIVO ADMINISTRATION OF

RETROVIRAL VECTORS EXPRESSING MOUSE INTERFERON-yAi-Li Shiau, Chih-Yun Lin, Men-Ya Chang, Tzong-Shin Tzai, Nai-San Wangand Chao-Liang Wu

GENE THERAPY IN COMBINATION WITH CHEMOTHERAPY 82

OR SURGERY FOR MURINE LUNG CANCER USING ADENOVIRAL

VECTORS ENCODING INTERLEUKIN-12

Nan-San Wang, Ai-Li Shiau, Jo-Lin Lo, Meng-Ya Chang and Chao-Liang Wu

GENE THERAPY FOR MURINE BLADDER CANCER USING 82

TRANSFORMING GROWTH FACTOR-P ANTISENSE

Chao-Liang Wu, Li-Ling Liu, Tzong-Shin Tzai, Nai-San Wang and Ai-Li Shiau

AFFINITY CAPTURE METHOD FOR IDENTIFICATION OF PEPTIDE 83

INHIBITORS OF CYCLIN DEPENDENT KINASES

Daniella I. Zheleva, Nikolai Z. Zhelev, Susan V. Duff, Weng Chan,

Adam Goldsworthy, Peter M. Fischer and David P. Lane

Subject Index 85

Author Index 91

xviii