LLTech atlas of images

7/30/2019 LLTech atlas of images

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LLTech

Light-CT Scanner

Atlas of images


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TABLE OF CONTENTS

BREAST Page 5

SKIN Page 11

CORNEA Page 15

PROSTATE Page 18

LUNG Page 23

KIDNEY Page 27

BRAIN Page 30

HEAD & NECK Page 35

COSMETOLOGY Page 39

CELLS Page 44

DEVELOPMENTAL BIOLOGY Page 49

PLANTS Page 61

CLINICAL APPLICATIONS RESEARCH APPLICATIONS


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CLINICAL APPLICATIONS

Back to Table of Contents


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BREAST AND LYMPH NODES- Extemporaneous analysis for cancer surgery

- Biobanking selection



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Grainy aspect of normal

fibrous tissue

Duct with

calcification

Lobule Adipocytes Vessel

Healthy breast tissue

Courtesy of Hpital Tenon, Paris, FranceAssayag et al, TCRT Express 1(1):e600254


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Ductal Carcinoma In Situ (DCIS)

Enlarged lobule

Duct with necrosis

Histology and LightCT images reveal the enlarged abnormal lobules and ducts characteristic of DCIS

Assayag et al, TCRT Express 1(1):e600254


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Lobular carcinoma

In-situ carcinoma cells

Invasive carcinoma cells

1 mm100 m

100 m

Tumorous cells are visible in in-situ and invasive carcinoma


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100m

Highly scattering

thin trabeculae

aspect: fibrous

tissue in the

tumourous part

Grainy medium

scattering aspect:

healthy fibrous

tissue

Invasive adenocarcinoma: nodular tumor

Interface of the tumourous and normal

appearing part :

Grey zones corresponding to foci

of carcinomatous cells

Tumour margin

500m

500m

Courtesy of Hpital Tenon, Paris, France

Assayag et al, TCRT Express 1(1):e600254


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Nodules containing

lymphoid tissue

Normal Sentinel Node

Adipocytes in hilum

Capsule Grieve et al Proc SPIE 2013


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Lymphoid zone

Invaded zone

Invaded Sentinel Node

Bright white aspect,

highly scattering dense

collagen meshwork

Grieve et al Proc SPIE 2013


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SKIN

- Extemporaneous margin analysis for cancersurgery




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Epidermis

Collagen

Pilosebaceous

unit

Sweat gland

Adipocytes

b

a

c

e

d

500 m

Normal skin morphology: vertical section

Dalimier and Salomon Dermatology 224, 84-92 (2012)


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Skin pathologies: basal cell carcinoma discrimination

at structural level

Dense peritumoral stroma



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Zoom on basal cell carcinoma:

discrimination at cellular level

200 m

100 m

Peritumoral stroma

High cell density

Courtesy of Hopitaux Universitaires de Genve, Genve, Switzerland


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CORNEA




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Surface epithelium Intermediary epithelium Basal epithelium

Bowmans layer Stromal keratocytes Endothelial cells

Cross sectional view En face views

Normal human cornea

100 m 100 m

LightCT imaging reveals the structures of normal cornea: epithelium, Bowmans layer, stroma, Descemets membrane,

and endothelium.

Use of LightCT in Eye Banks would allow graft cornea integrity to be assessed prior to transplant.Current practice examines only the epithelial surface and endothelial cells under a traditional specular microscope.


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Subepithelial fibrosis

Intraepithelial edema

LightCT cross section LightCT en face

Cornea with bullous keratopathy

Sub-epithelial

fibrosis

Optovue OCT in vivo on patientHistology

Histology, conventional OCT and Light CT views of the same region are compared.

The improvement in resolution with LightCT compared to conventional OCT is appreciated.

Pathological areas of subepithelial fibrosis and intraepithelial edema are identified.

Bowmans layer is clearly visible under the area of fibrosis.

150 m 150 m 100 m

Ghouali et alARVO (2013)


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PROSTATE

- Biobanking selection- Biopsy quality assessment



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1 mm

Human prostatic glands with hyperplasia


Zoom on hyperplasic gland

100 m


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3D imaging of glands

Stack of 150 images with 1 m step

50 m

50 m

50 m

sagittal

coronal

oblique

Sagittal reconstruction

Coronal reconstruction

Oblique reconstruction



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1 mm

1 mm

Biopsy imaging

Courtesy of Hpital Cochin, Paris, France


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Tumorous glands

200 m

Courtesy of Hpital Cochin, Paris, France


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LUNG




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Cross section of rat lung

Jain et al.J Pathol Inform 2011, 2:28.Courtesy of Weill Medical College, NY, USA

500 m


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Non-neoplastic human lung tissue

Alveoli

Blood vessel

Collapsed lung

Jain et al. Pathology Visions 2013.

Courtesy of Weill Medical College, NY, USA

500 m


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Adenocarcinoma of lung with lepidic & invasive

components

Lepidic

Invasive Invasive

Lepidic

InvasiveInvasive

500 m

100 m 100 m



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KIDNEY




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Non-neoplastic kidney tissue

Glomerulus

Tubules

Blood

vessel

Caron et al. Proc SPIE 2013

Blood vesselGlomerulus

Tubules

200 m


100 m

100 m

100 m


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Chromophobe renal cell carcinoma

Sheets of cells

Normal

architecture is

replaced by

sheets of cells

Sheets of cells


100 m

50 m


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BRAIN AND SPINAL CORD- Biobank selection

- In-vivo potential for surgical guidance


LightCT imaging of human epileptic brain and cerebellum identifies myelinated


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Neuron cell bodies (dark dots)

Axons/myelin fibers

Capillary

Cortex

White matter

Axon bundles

LightCT imaging of human epileptic brain and cerebellum identifies myelinated

axon fibers, a subpopulation of neuronal cell bodies and CNS vasculature.

Varlet et al 2013(submitted)

50 m

50 m

80 m

500 m

50 m

80 m

50 m

Hippocampus: Coronal section (left side)


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CA4 field

Hippocampal sulcus

Alveus

Sub-Ependymal

zone

CA1 field of the cornus ammonis

Stratum radiatus

of the CA1 field

Stratum granulosum of the dentate gyrus

Pyramidal neurons of the CA4 field

responsible for memory

Hippocampus: Coronal section (left side)

900 m

40 m

40 m

80 m

80 m


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Choroid Plexus Papilloma : Xanthomatous changes

Characteristic cauliflower-like masses

Empty papilla

Papilla filled with blood

Single layer of plexus cellsRare (0.5%) benign tumors

Diagnosis possible directly on FF-OCT imageVarlet et al 2013 (submitted)

50 m

50 m

20 m150 m

50 m

50 m

20 m


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LightCT images allow the distinction of meningiomas

from meningeal haemangiopericytoma

Collagen bundles

Whorls

Calcification

Collagen balls

Meningioma psammoma diagnosis possible directly on FF-OCT image

80% of meningiomas are benign.

Histologically, meningioma cells are relatively uniform, with a tendency to encircle one another, forming

whorls and psammoma bodies (laminated calcific concretions). They have a tendency to calcify and are

highly vascularized. Areas of fibrosis may be present.Varlet et al 2013 (submitted)

50 m

10 m

10 m500 m


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HEAD AND NECK TISSUE

- Biobanking selection- Extemporaneous margin analysis for

cancer surgery

- In-vivo potential for surgical guidance


Gl t500 m


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Glossectomy

Tumorous tongue tissue

Courtesy of Institut Gustave Roussy, Villejuif, France

Thin trabeculae aspect of

the fibrous tissue

Cell nuclei1 mm

500 m 500 m

Gl t


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Glossectomy

Mouth floor

Courtesy of Institut Gustave Roussy, Villejuif, France

Epithelial cell

nuclei

Spinous cells

1 mm

500 m 500 m

500 m


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RESEARCH APPLICATIONS



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COSMETOLOGY- In-vivo layer thickness measurement, cell count,

epidermis/dermis junction analysis, for ageing

and hydration evaluation

- Skin model analysis


En face skin slicing shows structural and cellular details


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Stratum corneum Stratum spinosum

Stratum basale Reticular region

Epidermis

Dermis

En-face skin slicing shows structural and cellular details

Melanin

papillary caps

Keratinocyte

nuclei

Blood vessels

Collagen

Corneocytes

100 m 100 m

100 m100 m

LLTech 2012 Courtesy of Hopitaux Universitaires de Genve, Genve, Switzerland

V ti l t ti f f li i


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Vertical reconstructions from en-face slicing:

Layer count and measurement

50 m

Stratum corneum

Stratum spinosum

Dermis

Human skin (in-vivo)

Skin model


Stack of images

-> vertical sections

Keratinocyte nuclei

Melanin caps at the

epidermis/dermis

junction

Blood vessel50 m

Evaluation of stratumcorneum thickness:

10 m


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Naevus imaging

500 microns

Dermal papillae

Hair follicule

100 m

50 m


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3D views

Wrinkle

Stratum corneum Stratum spinosum

Epidermis (separated by sodium bromide)

50 m

Reconstructed vertical slice


100 m

Corneocytes

Fingerprints (in-vivo)

Sweat duct


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CELL IMAGING- Cell count in native tissues and scaffolds

- Volume and shape analysis



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Adipocytes in human tissue

9 m depth 27 m depth 60 m depth

Reconstructed

depth slice

200 m

50 m

200 m 200 m


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Effect of fibrosis on adipocyte shape

Reconstructed vertical views of adipocytes in gel 3D modelling of adipocyte

No fibrosis

With fibrosis

50 m

50 m


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Cell scaffold

Scaffold en-face view

Scaffold reconstructed depth slice

100 m

100 m

Courtesy of University of Toronto, Canada

Hepatocytes

nuclei are visible


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Individual cells

100 m

Courtesy of Universit de Reims, France

Isolate collagen cells


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DEVELOPMENTAL BIOLOGY

- Time series analysis- Reconstructed depth slices and 3D imaging



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Developmental biology: Drosophila

200 m

Courtesy of ESPCI, Paris, France

In vivo imaging of drosophila melanogaster:


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automated time series over 72 hours

Prepupa (0-2 h)Transition to

pupal stage (24 h)

Pupal stage (48 h)Advanced pupal phase

before eclosion (72 h)


In vivo imaging of drosophila melanogaster


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100 um

4 days


Xenopus Laevis


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Otic vesicle

Cartilage

Xenopus Laevis

100 um

Pigmented cells

Courtesy of Muse National dHistoire Naturelle, Paris, France

Xenopus Laevis: back/tail


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Xenopus Laevis: back/tail

100 um

30 m depth 60 m depth

MyoseptumEpithelial

cells

Neural

cells


Xenopus Laevis


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Xenopus Laevis

50 um

Reconstructed vertical slices from stacks of images


Xenopus Laevis eye en face slices


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Xenopus Laevis eye: en face slices

Surface 10 m depth 20 m depth 30 m depth

40 m depth 50 m depth 60 m depth 70 m depth

80 m depth200 um

230 m depth140 m depth 180 m depth



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Copepod

100 um

Courtesy of Laboratoire de Rgulation des Signaux de Division, EA4479, University of Lille


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Zebra fish eye

100 m

100 m

En face slices

Reconstructed depth slice

Courtesy of Inserm, Paris, France

10 m depth 20 m depth 45 m depth


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Zebrafish brain female, 1 year old

50 um

Courtesy of Laboratoire Hubert Curien, Saint Etienne

Reconstructed view from stack of images

C Elegans Fast identification of anatomy


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30 m depth

ovocytes

intestine

100 m

C. Elegans Fast identification of anatomy

Body wall - cuticle

gonad arm

uterus100 m

15 m depth

LLTech 2012 Courtesy of ENS, Paris, France

3D reconstruction


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PLANTS

- High resolution reconstructed depthslices and 3D imaging


Leaf veins fresh leaf sample


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Leaf veins fresh leaf sample

Fibers

Veins

100 m

500 m


Apple


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Apple

100 m

Wax

Skin

Flesh

500 m

Water


Sorghum : cross section


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g

Wide field en face

14 mm x 15 mm

Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV), Montpellier RIO Imaging

Sorghum cross section: native field view


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800 m x 800 m en face field

Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV)Montpellier RIO Imaging

Sorghum cross section: native field view

Reconstructed

depth slices

500 m sample

thickness

Rice: hypocotyle


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Rice: hypocotyle

Wide field en face

5mm x 2mm

Reconstructed depth slice, 200um penetration depth

Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV), Montpellier RIO Imaging


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Contacts

LLTech, Inc.

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Princeton, NJ 08540, USA

Phone: +1 609 995 3506

LLTech

Ppinire Paris Sant Cochin

29 rue du Faubourg Saint Jacques

75014 Paris

Phone: +33 1 82 72 61 25

www.lltechimaging.com

[email protected]

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LLTech atlas of images