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1. Tomographic non-invasive imaging of C. Elegans worm:
LLTech Light-CT scanner
Sample preparation:
Imaging:
coverslip
Encapsulated worms
Microscope slide
5. Light-CT allows fast identification of anatomy worm 1
Image plane 1 @ 15m depth
Image plane 2 @ 30m depth
800m
800m
Corpus
pharynx
Isthmus
Posterior bulb
Excretory canal
spermatheca1
vulva
eggs
ovocytes
spermatheca2
gonad arm
intestine
ovocytes
6. Light-CT allows fast identification of anatomy worm 2
Image plane 1 @ 15m depth
Image plane 2 @ 30m depth
800m
800m
ovocytes
intestine
Body wall - cuticle
gonad arm
uterus
7. Light-CT allows full volumetric imaging in a few minutes
Light-CT image: allows fine
separation of structures
Standard microscope image
Optical sectioning:
1m thickness image
Light-CT images at different depths reveal fine structural
details
8. Light-CT allows fast, precise 3D rendering and volume
exploration
9. Images with 40x objective
Much higherresolution
10. Zoom on different parts
11. Scanning through the sample
Movieobtainedwith 3 microns steps
12. 3D viewing