Embed Size (px)
Citation preview
LIMOR MAN M.D, M.MED.SC
ASSISTANT PROFESSOR OF RESEARCH IN OBSTETRICS AND GYNECOLOGY
RONALD O. PERELMAN AND CLAUDIA COHEN CENTER FOR REPRODUCTIVE MEDICINE
WEILL CORNELL MEDICINE, NYC, NY, USA
•
•
•
• MINIMIZE ISCHEMIC INJURY TO THE
TRANSPLANT BY REDUCING THE LATENCY
PERIOD BEFORE NEOVASCULARIZATION
IMPROVE SURVIVAL
•
-Baird DT, Campbell B, de Souza C, Telfer E. Long-term ovarian function in sheep after ovariectomy and autotransplantation of cryopreserved cortical strips. Eur J Obstet Gynecol Reprod Biol. 2004 Apr 5;113 Suppl 1:S55-9.
•
-Dolmans MM, Martinez-Madrid B, Gadisseux E, Guiot Y, Yuan WY, Torre A, Camboni A, Van Langendonckt A, Donnez J. Short-term transplantation of isolated human ovarian follicles and
cortical tissue into nude mice. Reproduction. 2007 Aug;134(2):253-62.
0
20
40
60
80
100
InVIVO Postgra ing
%Primordialfollicles
primordial primary preantral antral
AMH AMH
FSH FSH
Metabolic demands
-Gougeon et al. Human Reproduction 1986
In vitro
activation
protocols
? Controlled
Ovarian
Hyperstimulation
(COH)
-Kawamura et al. PNAS. 2013.
•
•
•
•
ROBUST ENRICHMENT OF IGF-1 IN CULTURE
ng
/mL
P<0.001
Vectors-IGF-1
Human Endothelial Cells
0
200
400
600
800
1000
1200
1400
1600
ECs IGF-ECs
IGF-1
EXPERIMENTAL DESIGN
Human Ovarian Tissue
Fibrin clot
CONTROL GROUP STUDY GROUP
END POINT 3 WEEKS n=8 n=6
0
20
40
60
80
PrimordialPrimarySecondary
Blank
IGF
P=0.41 P=0.03 P=0.05
%
0
5
10
15
20
25
30
SecSimple Secpre-antral
Blank
IGF
P=0.22 P<0.001
%
CELL-BASED STRATEGY
-Gougeon et al. Human Reproduction 1986
In Vitro
Activation
Protocols
IGF-1
Co-
Transplantation COH
Daylon James
Laura Park
Jovana Lekovich
Glenn Schattman
Zev Rosenwaks
Nikica Zaninovic
Richard Bodine
Michael Ginsberg
