Life Imaging Center (LIC) - miap.eu · Leica STEDUV Stereo 3 room 00.024 LSM -I ... dualview FRET, EM-CCD Imaging 3 with definite focus, big incubator, LED illumination, 2 cameras

Life Imaging Center (LIC)

in the

Centre for Biological Systems Analysis (ZBSA) Albert-Ludwigs-University Freiburg

START LIC TOUR

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LIC Booking Calender

http://booking.zbsa.uni-freiburg.de/booking/cgi-bin/AGS_Login.exe?LaunchApp=EZ




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Infrastructure overview

Interactive Map of LIC (microscopes, lab and staff-offices)

The LIC in the ZBSA is app. 330 m² in 14 rooms. All lab space has biosafety level S2.

LIC – Office I LIC – Office II

Tissue Culture

room 00.025

Imaging 1

Imaging 2

LSM-U2

SD-TILL

LSM-I-2

room 00.017

LSM-I-

DuoLive

room 00.023

Leica

STED Stereo 3

room 00.024

LSM-I-

NLO2

Stereo 2

room 00.022

Develo

pm

ent

room

00.0

29

Imaging 3

room 00.030

Biostation 1 and 2

IncuCyte FLR

Imaging 4 room 00.028

LSM-I-

UV

AxioZoom

room 00.021

Wet Lab

molecular biology

preparation of

solutions

room 00.028

LIC-Office I

R. Nitschke

room 00.034

LIC-Office II Bierschenk,

Bodurova,Haxelmans, Jin,

Naumann

room 00.033 side entrance ZBSA

from Bio I

http://www.imaging.uni-freiburg.de/team.php











next back 5 Back to overview all Back to overview confocal


Microscopes for image recording

Inverted wide-field time-lapse / ratio imaging microscopes

Imaging 1 for calcium imaging or single color XFP, cooled CCD

Imaging 2 structured illumination (ApoTome), dualview FRET, EM-CCD

Imaging 3 with definite focus, big incubator, LED illumination, 2 cameras

Imaging 4 TIRF, FRET, multiple XFPs, dual camera

next back 6 Back to overview all Back to overview wide-field



Confocal microscopes (time-lapse, spectral, multi-location rec.)

LSM-I-2 Zeiss LSM 510 (inverted)

LSM-I-UV Zeiss LSM 510 META UV (inverted)

LSM-I-NLO2 Zeiss LSM 510 META NLO (inverted) 2-P laser

LSM-I-DUO-Live Zeiss LSM 5 Live DUO (inverted) high speed confocal

LSM-U-2 Zeiss LSM 510 (upright)

A1-CLEM Nikon LSM A1 CLEM (inverted)

LSM-U Zeiss LSM 510 NLO (upright) – in Bio I

LSM-I-NLO Zeiss LSM 510 META NLO (inv.) 2P laser – in Bio I

SD-I-ABL Zeiss Spinning disk with laser ablation - (inv.) - in Bio I

STED Leica Super-Resolution gated STED Leica TCS 8ST-WS




Special microscopes – long time-lapse machines up to 14 days

Biostation I Nikon Biostation IM with perfusion chamber

Biostation II Nikon Biostation IM

InCuCyte FLR Essen Instrument microscope inside incubator

Stereo fluorescence microscopes

AxioZoom Zeiss Axio Zoom V16 with B/W camera

Stereo 2 Leica MZ FL III with color camera

Stereo 3 Leica MZ FL III with color camera



Computerlab for data analysis

12 high-end PC DELL workstations for analysis and visualization

7 dual core PCs Office applications, E-mail

Software Imaris, Metamorph, Volocity, ZEN, Videomach and more

Servers for data storage and deconvolution

Huygens Core Server for deconvolution (128 GB RAM, 16 Processors)

SAN server storage capacity currently app. 60 TB


Computerlab for Data Analysis

12 high-end PCs (DELL) with Software for

Offline Analysis

•Server-based workstations with dual or quad

processors, 32 GB RAM,

•768 MB NVidia Quadro 4600 graphic card,

each with storage capacity of 4 TB

and 30 inch monitor (2560 x 1600)

7 dual core PCs for Office applications, E-

mail


List Of Installed Software On Workstations


Huygens Core Server for Deconvolution

To Hugens Deconvolution Server

http://cletus.lic.zbsa.privat/hrm/







live cell ratio imaging set-up for

• calcium, pH and GFP imaging with

monochromator (Till Photonics)

• cooled CCD camera (Roper CoolSnap

1300 x 1000),

• Software Metamorph / Metafluor

(Visitron, Molecular Devices)

(funded by DFG to Roland Nitschke)

Imaging 1 - inverted microscope Zeiss Axiovert 100

To Imaging 1 objectives and filters


Imaging 1 - objectives and optical filters

To Imaging 1


live cell imaging time-lapse imaging set-up for

• multi-location 3D-XFP and ratio imaging with

monochromator (Cairn instruments) or HBO

• excitation filter wheel (Ludl)

• Dual view for FRET imaging

• motorized xy-table for multi location and tile-image

recording

• Zeiss Apotome structured illumination for confocal

imaging

• two cooled CCD cameras (Roper Scientific

CoolSnap HQ 1300 x 1000) and (Axiocam

Rev.3, 1300x1000

• Software Metamorph/Metafluor (Visitron, Molecular

Devices) + Software Axiovision (Zeiss)

(funded by DFG to SFB 592 project Z2 + bioss Exc 294)

Imaging 2 - inverted microscope Zeiss Axiovert 200

To Imaging 2 objectives & filters


Imaging 2 - objectives

To Imaging 2 optical filter


Imaging 2 - optical filters

To Imaging 2 additional filters



To Imaging 2


live cell time-lapse imaging set-up for

• 3D-XFP ratio imaging with HXP

lamp or monochromator (Till Photon.)

• definite focus device

• fast Z-table (ASI)

• motorized xy- table for multi location

and tile-image recording

• two cooled CCD cameras (Axiocam

Rev.3, 1300x1000, and Axiocam

HS 640x480)

• Software Axiovision (Win-XP) or ZEN

blue (Windows 7, all Zeiss)

(industry collaboration R.N. and own funding

from R.N.)

Imaging 3 - inverted microscope Zeiss AxioObserver



Imaging 3 - objectives

To Imaging 3 optical filters



To Imaging 3


live cell time-lapse imaging set-up for

• multi-location 3D-XFP ratio imaging with TIRF

illumination or Sutter DG-4 wavelength switcher

• definite focus device

• motorized xy-table for multi location and tile-image

recording

• 2 cooled CCD cameras (Axiocam Rev.3, 1300x1000,

Software Axiovision (Zeiss)

• 1 Roper Scientific EM-CCD Cascade (512 x 512)

(FRISYS funding to M. Simons, TIRF from SFB592 and AG

Walz, colaboration with R.N.)

Imaging 4 - inverted microscope with TIRF



Imaging 4 - objectives and optical filters

To Imaging 4 more optical filters



To Imaging 4


4-channel confocal microscope with

• 458, 488, 514, 543 and 633 nm laser

lines,

• motorized xy-table for multi location

and tile-image recording,

• Software LSM 4.2 + ZEN 2010 (Zeiss),

NOTE !:

not useful for XFP combinations CFP + GFP,

GFP + YFP and CFP + YFP. Combination of

these XFPs alone with DsRed or similar one

however are possible.

(loan of Hautklinik – Prof. Bruckner-Tudermann

to the LIC)

LSM-I-2 - ZEISS LSM 510 inverted microscope Axiovert 200

To LSM-I-2 objectives & filters


LSM-I-2 - objectives

To LSM-I-2 filters


LSM-I-2 - optical filters

To LSM-I-2


3-channel + META detector spectral

confocal microscope with

• 351, 364, 458, 488, 514, 543 and 633

nm laser lines,



• Software LSM 4.2 + ZEN 2010 (Zeiss)

(funded by industry collaboration to R.N.)

LSM-I-UV - ZEISS LSM 510 inverted microscope Axiovert 200

To LSM-I-UV objectives & filters


LSM-I-UV - objectives

To LSM-I-UV filters


LSM-I-UV - optical filters

To LSM-I-UV




• 458, 488, 514, 543, 635 nm laser

lines

• tunable 2-photon laser Cameleon

Vision I, tuning range 690 - 1040

nm (Coherent),

• motorized xy-table for multi- location


• Software LSM 4.2 + ZEN 2010

(Zeiss)

(funded by DFG-HBFG to M. Frotscher and

R.N. + ZBSA)

LSM-I-NLO2 - ZEISS LSM 510 inverted microscope Axiovert 200

To LSM-I-NLO2 objectives & filters


LSM-I-NLO2 - objectives

To LSM-I-NLO2 filters


LSM-I-NLO2 - optical filters

To LSM-I-NLO2



confocal with

• 405, 458, 488, 489, 514, 532, 561,

635 nm laser lines

• two confocal scan heads LSM 510

Meta and LSM 5 Live for high-

speed imaging and specialized

for photo-activation and

conversion experiments,




(Zeiss)

(funded by DFG-Highlight to R.N. +

collaborators)

LSM-I-DUO-LIVE - ZEISS LSM 510 inverted microscope Axiovert 200

To LSM-I-DUO-Live objectives &filters


LSM-I-DUO-LIVE - objectives

To LSM-I-DUO-Live optical filters


LSM-I-DUO-LIVE - optical filters

To LSM-I-DUO-Live




• 405, 458, 488, 514, 561, 633 nm

laser lines,

• motorized xy-table for multi-location



(Zeiss)

(funded by DFG-Highlight to R.N. and SFB

592 project Z2

LSM-U-2 - ZEISS LSM 510 upright microscope AxioImager

To LSM-U-2 objectives & filters


LSM-U-2 - objectives and optical filters

To LSM-U-2


3-channel + spectral detector confocal

microscope with

• 405, 458, 488, 514, 561, 635 nm laser

lines,

• special dedicated to long time live cell

imaging by using the CLEM technology

and perfect focus system (PFS),

• motorized xy-table for multi-location and

tile-image recording,

• NIS 3.0 software (Nikon)

(funding by DFG to R. Baumeister)

A1 CLEM - Nikon inverted microscope

To A1 CLEM objectives and filters


A1 CLEM - objectives and optical filters

To A1 CLEM


4-channel confocal microscope with

458, 488, 514, 543, 633 nm laser lines,

motorized xy-table for multi location and tile-

image recording,

Software LSM 4.2 + ZEN 2010 (Zeiss)

Microscope located in Bio I 2nd floor Room

2055

(funded by grant to W. Driever + DFG to SFB

592 TP Z2)

LSM-U - ZEISS LSM 510 upright microscope Axioskop2 imaging

To LSM-U objectives & filters


LSM-U - objectives

To LSM-U optical filters


LSM-U - optical filters

To LSM-U


3-channel, + META detector

spectral confocal microscope with

• 458, 488, 514, 543, 635 nm laser

lines

• tunable 2-photon Cameleon laser

690 – 1020 nm (Coherent),




(Zeiss)

Microscope located in Bio I 2nd floor

Room 2055

(funded by DFG-HBFG to SFB 592 project

Z2)

LSM-I-NLO - ZEISS LSM 510 META inverted microscope Axiovert 200

To LSM-I-NLO objectives & filters


LSM-I-NLO - objectives

To LSM-I-NLO optical filters


LSM-I-NLO - optical filters

To LSM-I-NLO


Live cell time-lapse imaging set-up for

• multi-location 3D-imaging with Spinning

disk unit (CSU-22, Yokogawa),

• definite focus device for stable image

recording,

• motorized xy-table for multi-location and

tile-image recording,

• 2 cooled CCD cameras (Axiocam MRm

Rev.3, 1300 x 1040, Software

Axiovision (Zeiss)

• Ablation and photoactivation unit (Rapp

OptoElectronic) with 355 nm and 405

nm lasers

Microscope located in Bio I 2nd floor Room

2055

(funding by DFG-HBFG to W. Driever and R.N. and

BIOSS Exc 294)

SD-I-ABL ZEISS Spinning disk with inverted microscope AxioObserver

To SDI-I-ABL objectives


SD-I-ABL - objectives

To SD-I-ABL optical filters


SD-I-ABL - optical filters

To SD-I-ABL

next back 49 Back to overview all Back to overview special

dedicated long-time live cell time-lapse imaging

set-up ( 37

C +5 % CO2 ) for

• multi-location 2-channel fluorescence plus phase

contrast imaging,

• for multi-location and tile image recording

• Magnification 10 x, 20x, 40x and 80x

• Fluorescence filters for UV (Hoechst, DAPI), CYAN

(CFP), BLUE (GFP, Alexa 488), BLUE II (YFP),

GREEN (DsRED, rhodamine)

• Up to 4 independent experiment conditions

simultaneously possible,

• Maximal detection area 6 mm x 6 mm Flow chamber

add on for IBIDI flow slides or open glass bottom

or special IBIDI petri dishes

(funded by industry collaboration to R.N.)

Biostation I Nikon Biostation IM with flow chamber

To Biostation I & II optical filters


dedicated long-time live cell time-lapse

imaging set-up ( 37

C +5 % CO2 ) for

• multi-location 2-channel fluorescence plus

phase contrast imaging,

• multi-location and tile image recording

• Magnification 10 x, 20x, 40x and 80x

• Fluorescence filters for UV (Hoechst,

DAPI), CYAN (CFP), BLUE (GFP,

Alexa 488), BLUE II (YFP), GREEN

(DsRed, rhodamine)

• Up to 4 independent experiment

conditions simultaneously possible,

Maximal detection area 6 mm x 6 mm

• Usable for IBIDI flow slides (used with no

flow) or open glass bottom or special

IBIDI petri dishes

(funding by Ralf Baumeister group)

Biostation II Nikon Biostation IM with flow chamber

To Biostation I & II optical filters


Biostation I & II - optical filters

To Biostation I To Biostation II


InCuCyte FLR (Essen Instruments)

Fluorescence and phase contrast

microscope inside an incubator,

takes many different culture flasks.

Allows long time observation of your cultures.

The ideal tool for growth curve

measurements, confluence and transfection

rate measurements.

Two channel recording (480 nm excitation

and phase contrast).

Contact the LIC personal for further details of

usage.

(funding by Bioss Exc 294)

InCuCyte FLR - microscope inside conventional incubator


AxioZoom V16 - Fluorescence

Stereo-microscope with

• B/W camera AxioCam (1300x1000)

• Fluorescence excitation, transmitted

light and reflected light

(BF,BF+,RC,DF)



• PlanNeoFluar Z 1x0,25 FWD

53,1mm

• PlanNeoFluar Z 2,3 x0,57 FWD

10,6mm

(industry cooperation with ZEISS by R.N.)

AxioZoom - V16 Fluorescence Stereo-microscope with B/W camera


LEICA MZ FLIIII Stereo-Microscope

with

• Fluorescence excitation,

transmitted light and reflected

light

• Magn. 1 – 1000 x

• color camera AxioCamCRM Rev.3

1300 x1000 pixel)

• image recording software Axiovision

4.8.1

• timelapse option

• CO2 available at the microscope.

Contact the LIC personal for further details

of usage

Stereo 2 - LEICA MZ FLIIII Fluorescence Stereo-Microscope


LEICA MZ FL III Stereo-Microscope

with

• Fluorescence excitation, transmitted

light and reflected light

•Magn. 1 – 1000 x

•Color camera Idea 1300 x1000 pixel),

•mage recording software

VISIVIEW, time-lapse option

•no CO2 available at the microscope.

Contact the LIC personal for further details of

usage

(SFB 592 Z2 funding)

Stereo 3 - LEICA MZ FLIIII Fluorescence Stereo-Microscope


Lab Space Tissue Culture Room


Lab Space Tissue Culture Room


Lab Space Wet Lab I


Lab Space Wet Lab II


Lab Space Wet Lab III


End

We hope you enjoyed the tour.

The LIC Team

We are looking forward to help you with your research.


To STED objectives & filters

Super-Resolution gated STED Microscope Leica TCS 8ST-WS

Specifications: Inverted Leica DMI6000 Anti-Vibration table Z-Galvanometer Continuous wave lasers: VIS - Ar (65mW: 458, 476, 488, 496, 514nm) and UV - Diode 50mW:405nm) Pulsed laser (VIS - WLL avg.power 1.5mW: 470 - 670nm with integrated pulse picker = 78, 39, 19.5, 9.75MHz); Super resolution gated STED laser (Sub 50nm FWMH lateral resolution), Time Gated Detection Spectral Detection Range (400 - 720nm) Detectors (GsAsP hybrid detectors and high sensitive low noise, selected PMTs), Excitation Modulation (AOTF VIS: up to 8 channel and AOTF UV: up to 3 channel), Resonant Scanner 8kHz (Maximal Frame Resolution: 1024x1024 pixel; Scan-Zoom: 1.3 - 48x)

(funding via a grant to the Faculty of Medicine, Institute for Anatomy and Cellbiology, Department of Molecular Embryology, Prof. Dr. K. Krieglstein)

LEICA - Super-Resolution gated STED Microscope Leica TCS 8ST-WS


LEICA STED - objectives

To STED optical filters


LEICA STED - optical filters

To STED

Documents

Life Imaging Center (LIC) - miap.eu · Leica STEDUV Stereo 3 room 00.024 LSM -I ... dualview FRET, EM-CCD Imaging 3 with definite focus, big incubator, LED illumination, 2 cameras