Learning how to use the microscope

There are 2 kinds of

microscopes:

Simple: one lens (magnifying

glass)

Compound: 2 or more lenses

Parts of the Microscope

1. Revolving

Nosepiece

Parts of the Microscope2. Lens/Objective

Parts of the Microscope

3. Stage

Parts of the Microscope4. Diaphragm

(controls amount

of light)

1 = lowest setting/

5 = highest setting

Parts of the Microscope

5. Light

source

Parts of the Microscope

6. Base

Parts of the Microscope

7. Eyepiece

Parts of the Microscope

8. Arm

Parts of the Microscope

9. Stage Clips

Parts of the Microscope10. Course

Focus/

Adjustment

Parts of the Microscope

11. Fine

Focus/

Adjustment

12. Body

tube

Calculating Magnification

Multiply lens powers together!

• Eyepiece: 10 X by itself

• Scanning lens:

• 4X; red color

• Eyepiece X Scanning lens = total

• TOTAL magnification of 40 X

• mainly used for large objects/scanning

• Eyepiece: 10 X by itself

• Low Power: 10 X

• yellow color

• TOTAL magnification of 100 X

• Eyepiece: 10 X by itself

• High Power: 40 X;

• blue color

• TOTAL magnification of 400 X

• mainly used for smaller objects/details

Parts of the Microscope Clip

When you are ready to start:

Make sure it is on SCANNING power!The stage should be all the

way down (you focus by slowly moving it up!)Check the light setting

(should be at 1-2 to start)

Other Things to Consider:

Carrying the Microscope: Always use 2 hands!

Other Things to Consider:

Never use the COARSE FOCUS KNOB while on HIGH power.

When putting the microscope away (at the end of the day):

It should be on SCANNING power.The stage should be all the way

DOWN (do this with the course focus knob!)

The light switch should be OFF

TROUBLESHOOTINGWhat if you can’t get your object into focus?

• Check that you have one of the lenses clicked into place

• Check that your disc diaphragm is in place (not IN BETWEEN settings)

TROUBLESHOOTINGWhat if you can’t get your object into focus?

• Clean off your lenses using LENS PAPER. (Also, dirty slides are hard to focus on; so clean them, too!)

• GO BACK TO LOW POWER AND START OVER!

• Use a MAGNETIC field to bend beams o of electrons; used to see things at much higher level of detail

• Can magnify images up to 1,000,000times!

There are 2 kinds:

• SCANNING ELECTRON MICROSCOPES (SEM):Allows you to see the SURFACES of objects

There are 2 kinds:

• TRANSMISSION ELECTRON MICROSCOPES (TEM):Allow you to see THROUGH an object.

Electron Microscope Images:

http://www.pbrc.hawaii.edu/bemf/microangela/

Images through an electron microscope

Simple vs. Compound VideoClip

Microscopes by Brainpop

Roll

Assignment 10/13

ONLY WRITE THE QUESTION!

1. Cell Theory states all of the following except:a. Cells are the basic units of life

b. All organisms are made up of one or more cells

c. Atoms and molecules are the smallest forms of life

d. All cells come from cells that already exist.

2. List the 5 Scientists we learned in class and their contribution to

Science.

(DO NOT USE YOUR NOTES OR JOURNAL.. USE YOUR

MEMORY!)

Zaccharias Janssen- Eyeglass maker/ first compound microscope

Anton Van Leewenhoek- looked at pond water with a tiny glass bead

Robert Hooke- Discovered that cork was made up of little boxes called cells

Mattias Schlieden- Said all plants were made of cells

Theodor Schwann- Said all animals were made of cells

Rudolph Virchow- Said all cells divide to make new cells