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THE MICROSCOPE

Use of the Microscope

Lab Manual

Exercise # 2

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THE MICROSCOPE

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THE COMPOUND MICROSCOPE

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BASE

• It contains:• Off on switch• Voltage regulator• illuminator

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STAGE

• It supports the microscope slides and contains slide clips and mechanical stage

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MECHANICAL STAGE• It has 2 knobs for

movement of the slides from left to right and front to back

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SUBSTANCE CONDENSOR

• It has 3 parts:• blue filter• iris diaphragm• condenser lens

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• Blue filter:• the short wavelength of

the blue light provides maximum resolution

• Iris diaphragm:• controls the amount of

light passing from the slide source

• Condenser lens:• collets and focuses the

light from the illuminator onto the slide

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LENSES

• They magnify the image• Objectives: they have 3

or 4 objectives • 4X scanner• 10X low power• 40X high dry• 100 X oil emersion lens• Ocular lens or eyepiece:• it is 10X and it can have

a scale

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ADJUSTMENT KNOBS

• They are for focusing• Coarse adjustment knob:• for locating and focusing

objects (DO NOT USE for high magnification)

• Fine adjustment knob: (small)• for focusing on higher powers

knobs

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ARM• Supports the objective

lens

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CARES OF THE MICROSCOPE

• When moving the microscope, carry it with 2 hands (one hand to grip the arm and the other under the base

• Lenses have to be clean with lens paper (to keep them free of oil and dust).

• Do not use the coarse adjustment when focusing with the higher power objectives

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PROCEDURES• 1- Turn on the illuminator using the on/off switch

• 2- Turn the nosepiece to bring the 4X objective (scanner) into position

• 3- Raise the stage into its highest position

• 4- Place a slide of the letter “e” in the slide clamp on the stage

• 5- Turn the coarse adjustment knob to bring the “e” into focus

• 6- Measure the field (the brightly lighted circle that you see when you look through the ocular lens)

• 7- Center the ”e” in your field of view and then rotate the nosepiece to 10X

• 8- Use the fine adjustment knob to focus until the image is sharp. Draw the image. Do not use the coarse adjustment

• 10- Rotate the nosepiece until the 40X. Draw the image

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SQUAMOUS CELLS

• Simple squamous peritoneum

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CUBOIDAL CELLS

• Simple cuboidal kidney

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COLUMNAR CELLS• Simple columnar small intestine

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REMEMBER!

GO TO THE TUTORING ROOM AND PRACTICE WITH MODELS