Jasco Fp920 Manual 1995 Eng

7/22/2019 Jasco Fp920 Manual 1995 Eng

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MODEL FP-920Intel l igentFluorescence Detector

Operation Manual

MODE S OttM. REBPGN9E

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1 I2

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P ar t N o . 0 3 0 2 0 2 0 5 B R e v . 1 9 9 5 . 0 21 9 9 4 . 0 1


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Operation M anual

WARRANTYProducts sold by JASCO, unless otherwise specif ied, are warranted for a periodof one year from the date of shipment to be free of defects in materials andworkmanship. If any defects should occur in the product during this period ofwarranty, JASCO will repair or replace the defective part(s) or product free ofcharge.Products and parts may not be returned which are contaminated and mayconstitute health hazards to JASC O em ployees.This warranty does not apply to defects resulting from the following:1) Improper or inadequate operation, maintenance, adjustment or calibration.2) Una uthorized modification or misuse.3) Use of consum able parts that are not supplied by JA SCO .4) A ccident beyond JA SCO's control.5) Disaster.6) Corrosion due to the use of improper solvents or samp les.This warranty does not cover the consumable parts listed below:1) Deuterium lamp, tungsten lamp, xeno n lamp and other light sources .2) Mirrors in the light source section, and cell window s.3) Fuses, batteries, glassware, chart paper and ink.4) Plunger seals, needle seals, cell window gaske ts, valve seals, disk seals andother sea l materials.5) T ubing and fitt ings such as ferrules and nuts, f i lters such as inlet f i lters, linefilters and other solvent f i lters.6) Pre-columns and guard columns.The warranty period for all parts and repairs supplied under this warranty expireswith the warranty period of the original product. For inquiries concerning repairservice, contact your JASCO agent after confirming the model name and serialnumber of your instrument.

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Operation Manual

INTRODUCTIONThis instruction manual describes the operation of the JASCO Model FP-920Intelligent Spectrofluorometer. Carefully read this manual to obtain a thoroughunderstanding of the software before using it in conjunction with the MD-910Detector.Special considerations and precautions for safe and efficient use are alsodescribed throughout the manual. These appear in the following forms:WARNING Warns of potentially hazardous situations and outlines the correctprocedures or practices required to prevent personal injury.CAUTION: Alerts the operator to the correct operating or maintenanceprocedures required to prevent instrument failure or damage.Note: Provides additional information to aid the operator in obtaining optimalinstrument performance.Pressurized, hazardous solvents are used in high performance liquidchromatography. Take care to follow proper laboratory procedures to ensureoperator safety. A lways wear eye, skin and clothing protection when operatingthe instrument, especially during sample injection, valve opening, etc.

NOTICEThis instruction manual is believed to be complete and accurate at the time ofpublication. In no event shall JASCO be liable for incidental or consequentialdamages in connectionwith,or a rising from, the use of this manual.The contents of this manual are subject to change without notice in accordancewith product improvements.

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Table of Contents1 . Unpa cking and installation 1

1-1 Unpacking and instrument self-check 11-1-1 Ma in body 11-1-2 Stand ard A ccess ories 1

1-2 Installation 21-2-1 Installation conditions 21-2-2 Installa tion 3

1-3 Turning on the power 41-4 Operation check 51-5 Abo ut safety 6

1-5-1 Insta llation 61-5-2 G rounding 61-5-3 Light source cooling fan 61-5-4 Fu se .... 6

2. Nam es and Functions of Parts 82-1 Front panel 8

2-1-1 Switches and keys 82-1-2 Keypa d 102-1-3 Flowcell section 142-2 Back panel 15

3. Key Operations in Normal Operation Mode 183-1 Parameter sett ings 183-1-1 Wa velength sett ing 203-1-2 G ain setting 203-1-3 A ttenuation setting 213-1-4 R esponse sett ing 223-2 Special param eter settings 22

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4. Spe cial Key Operations ([SHIFT][n] operations) 234-1 Preampiified output ([SHIFT][1] setting) . . .234-2 Lamp off timer ([SHIFT ][2] setting) 24

4-2-1 T imer set 244-2-2 R elighting the lamp 254-3 Signal filter meth od ([SHIFT][3] setting) 264-3-1 Setting method 264-3-2 Method of use 274-4 Autozero operation ([SHIFT][4] setting) 294-4-1 Opera tion method 294-4-2 Setting method 294-4-3 Metho d of use 304-5 Integrator output zero point shift ([SH FT][5] settin g)... 32

4-5-1 Setting method 324-5-2 Method of use 344-6 Record er outpu t polarity {[SHIFT][6] setting) 35

4-6-1 Setting method 354-6-2 Method of use 364-7 Xenon lamp use time ([SHIFT][7] setting) 374-8 Spectrum bandwidth (ISHIFT][9] setting) 384-8-1 Setting method 384-8-2 Method of use .394-9 Program number ([SHIFT][PRGM ] setting) 404-1 0 Wavelength range ([SHIFT][CLEAR ] setting) 414-1 1 Temperature correction ([SHIFT][MAR KER] setting) 43

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5. Key Operations in the Program Mode 445-1 Outline 445-2 Switch to program mode 445-3 Program mode mon itor screen 455-4 Input method for initial program parameters 465-5 Input metho d for program step 1 and after 49

5-5-1 N ew input (used if step is blank) 505-5-2 Edit method(for changing time and parame ters) 545-5-3 Edit method (for chang ing only values ) 555-6 Program editing metho d 565-6-1 Step insertion 56

5-6-2 Step deletion 585-6-3 Section deletion (delete thedesign ated step and all steps thereafter) 605-7 Program examp le 625-8 Spectrum measurem ent in the program mo de 645-8-1 Outline 645-8-2 Setting method 655-8-3 Execution exam ple 685-8-4 Spectrum output 69

6. Operation 706-1 Operation in normal operation mod e 706-1-1 Parameter settings 706-1-2 Error mess ages 706-1 -3 Measures to take i f the [AU T OZER O] keylamp is f lashing or the "W A R N INGA UT OZER O" message is d isp layed 726-2 Operation in the program mod e 736-2-1 Initial settings 736-2-2 Starting the program 756-2-3 Chan ging parame ters during a program run 766-2-4 Stopping the program 77

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7. Wavelength Scan Mode 787-1 Outline of functions 787-2 Outline of operation. 787-3 Notes for use 797-4 Spectrum measurement 807-5 Spectrum output 81

7-5-1 Key opera tions 817-5-2 Output form 837-6 Difference spectrum outpu t 847-6-1 Key operations 847-6-2 Output form 867-6-3 Method of use 867-7 Spectrum measurement parameter settings 877-7-1 Key operations 877-7-2 Settings 89

8. Specifications and Options 908-1 Specifications 908-2 Options (Optional accesso ries) 92

9 . Optiona l Accesso ries 939-1 Filter types and uses 939-2 Square cell holder 959-3 High-sensitivity flowce ll 959-4 Micro-flowcell 959-5 Fluorescent photom ultiplier types 95

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1 . Unpacking and InstallationThis sect ion descr ibes the proper procedure for unpacking and installing theinstrument.

1-1 Unpackingandinstrumen t self-checkThis sect ion descr ibes the unpacking and self-check procedures of theinstrument.

1-1-1 Main bodyOpen thecardboardbox and carefully remove the instrument. Confirm thatthe serial numberon theplateon thesideof themain bodyis thesameasthe serial number in the inspection table, andthat the power voltage is asdesignated See Fig. 1-1).

MODELS E R I A LNo.D A T EL I N E VTG.

PROTECTJ S O Corporator

F P- 920A398777OCT.1993

AC100V~120VAC200V~240V

CLASS I192 JHMAZ**

50/60HZ50/60HZ

MADG2

IN

5 A. 5 AJAPAN

Figure1-1 Serial Number Plate

1-1-2 Standard AccessoriesTakeout the accessories and check each part against the detailed list ofstandard accessories. If there is a missing or damaged part, contact yournearest Jasco representative.

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1-2 InstallationT his section describes the installation of the instrument.

1 -2-1 Installation conditionsSince the high speed liquid chromatograph uses a large quantity of solventcompared to most analytical instruments, its installation location must be selectedwith consideration for possible solvent spills and solvent evaporation. Payparticular attention to the points listed below.

1) Install the instrument in a well ventilated area2) Be aware of fire hazards3) Have a sink nearby4) Shield the instrument from direct sunlight5) Choose a location with little temperature fluctuation6) Prevent air conditioning and heating from directly hitting the instrument7) Use a location with little vibration8) Use a power voltage within +10% of the designated voltage(+5% is preferable)9) Use a location with no strong magnetic fields or high frequency waves10) Use a location with little dust11) Be sure a firm grounding is m aintained

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1-2-2 InstallationInstallthenstrumenton abase that ca n withstand itsweight, and that ismatchedtothewidth and depthofthe unit. Fig .1-2showsanexample of anapp ropr iatearrangement.

1 1A

( o ) 1vO O

^Clearance at least 10mmFigure1-2 Arrangement Example

Note: SincetheFP-920 givesoffheat,do notplace other devicesoneither sideof the instrument. Inparticular, besuretom aintainaclearance of at least10mmonhe left side.

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1-3 Turningon thepowerCAUTION: Before connecting the power cable, confirm that the main power

switch is OFF, and that the power voltage selector located on therear panel of the instrument is set in accordance with the voltagesupplied by the power source.

Turn the power on using the sequence shown below:1) Turnthe"POW ER " swi tchto"OFF".2) Insert the receptacle end of the power cable into the power input terminal(seeFig. 2-4) on therearof the instrument.3) Inserttheplugend of thepower cable intothepower socket.4 ) Turnthe"POW ER " swi tchto"ON".

WARNING(1 ) Useeitherathree-pronged grounded socket, or atwo-pronged socketinconnection with a ground adaptor. Securely ground the wire on theadaptor. If it is notpossible toground theinsturment ineither of theseways, use the GND ground terminal on therearof theinstrument toground theunit.(2 ) Do notattempt toground theunitto water piping, since thepiping usedis often madeof anon-co nducting material (vinyl chloride).(3 ) For safety reasons, do not use gaspipesas a ground.

If theinstrument is notgrounded, thereis theposs ibility of an electricshock in theunlikely eventof abreakin theunit's insulation.Note: The voltage measured by a voltage meter with high input impedancebetween theground terminal GND on therearof theinstrument and theearthmay becloseto thepower voltage w ithouta ground.

This phenomenon is caused by the noise filter connected between theground terminaland thepower lineand s not an electrical short circuit.Thecurrent flowing through thenoise filteris 1mA orlessand is not dangerousto thehumanbody.

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1-4 Operation checkW hen the FP-920 power is turned on, the message shown below wil l be displayedon the LCD for about 1 second , after which the instrument will beg in a s elf-checkprocedure.

FP-920JASCO Ver .Lxx

Figure 1-1 Screen when Power is T urned On

The items covered in the self-check are listed below:(1) R OM, RA M, BA CK UP (Memory check)(2) +/- 15V, +5V, (DC power check)(3) LA MP (Light source lamp check)(4) G , DRIVE (Wavelength dr ive sect ion check)If the self-check for a given item reveals no abn ormality, "OK " will be displa yedfor 0.5 seconds and the instrument will continue to the next item. If an error isdetected, a message will be displayed corresponding to the item in which theerror occurred.Press [SHIFT][V] to move from the item where the error occurred to the next item.The instrument will proceed with the next self-check. At the end of the self-check,the monochromator spectrum bandwidth and the lamp use time will be displayed.If the self-check ends normally, the monitor screen for the normal operation modewill be displayed. The setting values such as wavelength and sensitivity will bethe sam e as those set when the pow er was last turned off.

NORM 0.0000 STD300 500 1000 16

Figure 1-2 Monitor Screen for the N ormal Operation M ode

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1-5 About safetyT his section contains information regarding the safe operation of the instrument.

1 -5-1 InstallationCompared to other analytical instruments, the high speed liquidchromatograph uses a large quantity of solvent. Some of these solvents arevolatile, toxic or flammable. While care should be taken to find a wellventilated location for the instrument, it is also necessary to be prepared forpossible solvent spills or leaks from piping joints.

1-5-2 GroundingBecause solutions norma lly flow through the internal parts of the high speedliquid chromatograph, there is the danger of an e lectrical short circuit in theunlikely event of a fluid leak within the instrument.For the safety of the operator, even in the event of an e lectrical short circuit,be sure to ground the instrument before use. Refer to section 1-3 for detailsabout grounding the unit.

1 -5-3 Light source cooling fanCheck that the light source cooling fan on the back of the instrument (seeFig.2-4) is turning properly when the power is turned on. If the lamp is onwhen the fan has stopped, the glass portion of the lamp will fatigue rapidly.

1-5-4 FuseThe following warning label is attached to the instrument. Do not remove orobscure the label.

Note: If i t is not possible to read the label, contact your nearest Jascorepresentative.

For protection from dangers such as fire, use fuses of the designated currentvalue.

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LJ

W A R N I N GFOR CONTINUED PROTECTIONAGAINST RISK OF FIRE,REPLACE ONLY WITH FUSEOF THE SPECIFIED TYPEAND CURRENT RATINGS.

Figure1-5 Warning Labelon theBack Panel

Warning: Toprotect peopleand theinstrument from dangers such as fire,use only fusesof thedesignated current value. Also, to preventelectric shocks, besure to turn the "POWER" switch to OFFand unplugthepowe r cable before replacing fuses.

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2 Names and Functions of PartsThis chapter containsthenamesandfunct ionsoftheunit 's parts.

2-1 Front panelT his sec t ion describesthe ront paneloftheunit.

2-1-1 Switchesandkeys

FLowcelIsection LCDdisplay

fclCDCD

''PO WER ' s w i t c h E d i t k e y s / C o n t r oI k e y s /Figure 2-1 Front Panel

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Par t Name

LCD Display

"POWE R " sw itchFiowcell sectionEdit keys

Control keys

Descr ipt ion

Displays operating conditions, f luorescent intensity,parameters, setting values, and various messages.Used to turn the instrument on or off.This is the cassette type fiowcell section.Used to select parameters, set numerical values and createprogramsUsed to operate detector fuctions such as autozero, markerand time program

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2-1-2 KeypadT he keypadisillustratedin Fig. 2-2.

M O D E S IG N A L R E S P O N S E

EX nm) EM nm) G AIN A TT E NSHIFT SCAN PRGM

|E M SP.l

A T T E N

4W.L.11

EN D0

EX SP .8RSPNS

5W.L.22

SLIT9A . Z E R O

6G A I N3

A

CLEAR

EDIT/ENTER

PRGMRUNAUTOZERO

MARKER

MONITORFigure2-2 Keypad

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Key Function

[PR G M RUN ] - Used to start and stop the t ime program. If pressed when theupper left lamp is off, the time program will start and the lampwill l ight. If pressed again the time program will stop and thelamp will go out.- Used for spectrum measurement and output.

[AU TO ZER O] -Sets the integrator and recorder outpt its to zero.

[MA RKER]

[MONIT OR]

[ P R G M ]

[SC A N ]

[SHIFT]

-A marker will be added to the recorder output (about +3mV).

- Used to return to or change the monitor screen.

Note: [MO NITO R] displays the instrument operationconditions such as wavelength, fluorescentintensity and program running time.

-Us ed to switch between the normal operation m ode and t imeprogram m ode.

-Used to switch to the wavelength scan mode (press[MON ITOR ] to return to the or iginal mode).

- Used in combination with the numeric keys to set parametersthat are not frequently changed.

[EN D/0] [ .] - [SLIT/9] -N umer ic keys

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Key

[W.L1/1]

[W.L2/2]

[GAIN/3]

[ATTEN/4]

[RSPNS/5]

[A.ZERO/ 6]

[EMSP./7]

Function

- N umeric 1 key-Wavelength change key in the normal operation mode-Use d for "EX, EM, W.L" (W.L is short for wavelength)functions in time program mode

- Numeric " 2" key

- Numeric "3" Key- Gain change key in the normal operation mode-Used for the "G A IN" function in time program mode

- N umeric " 4" key-A ttenuation change key in the normal operation mode- Used for the "AT TEN " (attenuation) function in time programmode

- N umeric "5" key- Response (equivalent speed) change key in the normaloperation mode

-Use d for the "R SPN S" (response) function in time programmode

- Numeric "6" key- Used for the "A .Z" (autozero) function in time program mode

-Num eric "7 " key-Used for the "EM SP" (emission spectrum) function in timeprogram mode

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Key

[EXSP./8]

[SLIT/9]

[A] fV]

[EDIT/ENTER]

[CLEAR]

Function

-Numeric "8 " key- Used for the "EX SP" (excitation spectrum) function in timeprogram mode

- Numeric "9 " key- Used for the "SLIT " (fluorescence spectral bandwidth)functions in time program mode

- Used to select setting values (ATTEN for example)- Used to increase or decrease step numbers in the timeprogram.

- Used to check or change setting values.

- Used to cancel input values-Used to clear problems (LEAK IN CELL for example).

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2-1-3 F lowce l l section

Ce I I p an e IFigure 2-3 Flowcell Cassette (Standard)

NameCell bodyFlowcellCell panelLock screw

ExplanationFlowcell support bodySilica glass f lowcell

Cell panel attachment screw

Cau tion: Wh en attaching the flowcell section, press the cell panel in byhand so that the guide pins go through the back holes. Next,alternately tighten the lock screws to attach the p anel. If the panelis inserted with the lock screws tightened down from thebeginning, the panel may not be positioned properly.

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2-2 B ack panelT he external appearance of the back panel is shown in Fig. 2-4.

Input/output R S -232Cterm inal L C-N et termin al

Light sourcecooing fan

\ G round \ L ine voltagePower inlet\termina \selector Power fuseFigure 2-4 Back Panel

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Name DescriptionInput/output terminal

Signal output terminal

REC

INT

M A R KOUT

LEAKOUT

G(GND)

G ( G N D )

Signal input terminalMA R K IN +G N D

A / Z I NIN

PRGM RST /STG N D

Recorder output terminal (The output level orsensitivity is determined by the A T T EN setting)

Integrator output terminal

Marker output terminal (Normally "OPEN", thepoint of contact is "CLOSED" if the [MARK] key ispressed or if "MAR K IN " is input.)Fluid leak warning output terminal (Normally"OPEN", the point of contact is "CLOSED" if a f luidleak occurs in the flowcell.)

Note: O nly the buffer solution fluid leaks canbe detected.

Marker input terminal (A marker input signal isadded to the recorder output if the point of contactsignal is "CLOSED"). Refer to section 4-4 fordetailsAutozero input terminal (The recorder output andintegrator output are zeroed if the point ofconnection signal is "CLOSED").Time program input terminal(If the point of contact is "CLOSED", the programstarts immediately after time program is reset(return to initial conditions)

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N a m e Description

Light source cooling fan

Power inlet

Power fuse

Ground terminal

Line voltage selector

RS-232C terminal

Fan for cooling the xenon lamp

Power cable connection.

Warning: For continued protection againstthe risk of fire, replace old fuseswith fuses of the specified typeand current ratings only.

T his is the ground terminal for the power l ine. T hisis not a ground terminal for the input/output signal.(Do not use this in place of the input/outputterminal ground.)

Switches the l ine voltage between 115V and 23 0V115V : 85V- 132V230V : 187V- 264V

Instrument control and digital output of signals ispossible through the RS-232C channel.

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Operation Manual

3. Key Operations in Normal Operation ModeIn the normal operat ion mode, chromatograms are taken at a f ixed wavelengthand range.

3-1 Param eter settingsThe screen shown in Fig. 3-1 wil l appear af ter turning the power on, when theself-check is complete.

N OR M 0.0000 STD300 500 1000 16

Figure 3-1 LCD Screen after Com pletion of Self-ch eck

The sett ing values for range, wavelength, and response t ime wil l be the same asthose set when the power was last turned off.

N ormal operation F luorescent R esponse speed (RES PON SE)mode intensity 0.0013 Standarc

Excitationwavelength300nm

EX (nm )/ \ EM(nm)

Emissionwavelength500nm

Gain1000

At tenuat ion16

Figure 3-2 Descr iption of the Normal Operat ion Mode Screen18 Jasco FP-920


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T he m ethod for setting parameters is shown below:1) W he n the key designa ting the parameter to be chan ged is press ed, the setvalue w il l flash.2) Input the settings using the [A]/[V] keys or the numeric keys. If an incorrectvalue is input, press [CLEAR] and input the value again.3) Press [EDIT/EN TE R ] and the f lashing wil l stop.

A list of key operations for setting parameters is shown in Tab le 3- 1.Sections 3-1-1 to 3-1-4 show actual examples.

T able 3-1 . Key Operat ions for Sett ing Parameters

Parameter

Wavelength(EX, EM)

Ga in

Attenuation

Response

Setting key

[W.L1/1]

[GA IN /3 ]

[ A T T EN /4 ]

[RSPNS/5 ]

Change method

Numeric keys

N umer ic or[A]/[V] keysNumeric or[A]/[V] keys

[A]/[V] keys

Sett ing range

200 - 900 (nm)

1, 10, 100, 1000

S, 256 , 128, 4, 2, 1

FST, STD, SLW, or3S ,5S , 10S, 20S, 40S

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3-1-1 Wavelength settingSet the excitat ion wavelength to 230nm and the emission wavelength to 350nm[W.L1/1] -> [W.L2/2][GA IN/3] [EN D/0] -> [EDIT/ENT ER ]Param eter selection Input numbers Conf irm

-> [GA IN/3][RSPN S/5][END/0] -> [EDIT/ENT ER ]Input numbe rs Confirm

Note 1: When [SHIFJJ[W.L1/1] is pressed, the screen w ill change to the preamp.output screen. When [MO NITO R] is pressed, the screen will return to thescreen shown in Fig. 3-1.

Note 2: The wavelength setting range can be broadened using special settings(refer to section 4-10).

3-1-2 G ain set t in gChange the gain to 10[GAIN /3 ]Parameterselect ion

[W.L1/1] [END/0]Input numbers

Select numbers

[ED IT /ENT ER ]Conf irm

Note: When [SHIFT][GAIN/3] is pressed, the screen w ill change to the signal filtersetting screen.When [MO NITO R] is pressed the screen will return to the screen shown inFig. 3-1.

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3-1-3 Attenuation settingSet the attenuation to 256

[A T T EN /4 ]Parameterselect ion

[W.L2/2] [RSPNS/5] [A.Z/6]Input numbers

[ED IT /ENT ER ]Conf irm

Select numbers

Note: W hen [SHIFT][A TT EN /4] is pressed, the screen wil l change to the autozerooperat ion screen.When [MONITOR] is pressed, the screen wil l return to the screen shown inFig.3 - 1 .

The d i f ference between GAIN and ATTEN:The magnitude of the voltage added to the f luorescence detectorphotomultiplier tube is called gain. Larger gain values mean that a largervoltage is added, and higher sensit ivi ty is obtained. Adjustment using gainaf fords a wide dynamic range.

On the other hand, a decrease in the recorder output is called attenuation.Smaller attenuation values produce smaller decreases and higher sensitivity.Adjustment using attenuation enables the decrease in recorder output to befinely and accurately set.

Note: Attenuation h as no influence on integrator output, integrator output can beconsidered as recorder output with the attenuation fixed at 1. Recorderoutput is zero when attenuation is set to "S".

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3-1 -4 Response settingChange the response setting to SLOW

[RSPN S/5] ^ [A] /[V ] [EDIT/ENT ER ]Param eter Select setting Confirmselect ion

Note 1 : Whe n [SHIFT][RSPN S/5] is pressed, th e screen w ill change to the zero-point shift screen. When [MONITOR] is pressed, the screen will return tothe screen shown in Fig. 3-1.

Note 2: O nly values can be set here. The response method (single filter method)is set in the screen shown in Fig. 4-3 (see section 4-3).

3-2 Spec ial parameter settingsFunction parameters that are not frequently used, and parameters that are rarelychan ged o nce set, cannot be set in the screen shown in Fig. 3- 1.The special parameter settings screens can be accessed from the screen shownin Fig. 3-1 by pressing [SHIFT] and a numeric key. After setting in the specialscreen, press [MONITOR] to return to the screen shown in Fig. 3-1 (refer tochapter 4 for a more detailed explanation).

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4. Special Key Operations ([SHIFT][n] operations)The functions described in this chapter can be executed from the screensdisplayed by pressing the [SHIFT] key together with a numeric key from themonitor screen. (These screens wil l be framed with double l ines in the figures ofthis manual)After making the settings, press the [MONITOR] key to return to the monitorscreen.

Note: There are also functions that use keys other than the numenc keys.

4-1 Pream plified outpu t ([SHIFT ][1] setting)The preamplified output wil l be displayed on this screen. This is used tocheck for deterioration of the lamp or light source mirror and for theexistence of bubbles in the cell. The output is also used for re-alignmentafter replacing the Xe lamp.

Preamplified output screen EX: 2.603VEM: 1.865V

T[SHIFT][W.L1/1]

EX: Indicates the excitationlight intensity

EM: Indicates the intensityof f luorescence fromthe f lowcel l

[M ON ITOR ]

Monitor screen NORM 0.0000 STD300 500 1000 16

Figure 4-1 Displaying the Preamplified Output

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4-2 Lamp off timer ([SHIFT ][2] setting)T his section outlines the use of the lamp off timer

4-2-1 Timer setThe lamp will be turned off after the time set on this screen has elapsed.

Lamp off timer screenLAMP OFF TIMER

1.0 HOUR(S)

[SHIFT ][W.L/2] [MON ITOR ]

Monitor screen NORM 0.0000 STD300 500 1000 16

[CLEAR]LAMP

TIMER OFF

This message will bedisplayed and thelamp will be turnedoff after the set timehas elapsed.

Figure 4-2 Operation of Lamp Off Timer

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The method for setting the timer is shown below:1) Press [EDIT/EN TE R ] and the number wil l f lash.

{Example} OFF ( f lashing)2) Input a num eric value. If an incorrect nume ric value is ente red, press [CLE A R ]

and re-enter the input.{Example} Change to [1] -> 1.0 HOUR (S) (Flashing)

3) Press [EDIT/ENT ER ] and the number wil l stop f lashing.{Example} 1.0HOUR(S)

Note: The setting range for the lamp off timer is from 0 (OFF) to 99.9 H (in 0.1 Hincrements).The lamp off timer is automatically set to "OFF" when the power is turnedon .

4-2-2 Relighting the lampT urn the power of f then on again.

Note: It is sometimes difficult to relight the lamp imm ediately after the lamp hasbeen turned off. If the lamp will not light, wait for at least one minute, andturn the power on again.

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4-3 Signal Filter Method ([SHIFT][3] setting)This section describes the signal f i lter method.

4-3-1 Sett ing methodThe noise reduction method (Note) for the fluorescence signal is set on thisscreen.

Signal f i lter method screen

[SHIFT] [GAIN/3]

SIGNAL F ILTERCR FILTER

[ M O N I T O R ]

Monitor screen NORM 0 .0000 STD300 500 1000 16

Figure 4-3. Signal Filter Metho d Display

Note: O nly the signal filter method can be set in this display. Values are set in themon itor screen (screen displayed in Fig. 3-1) (refer to section 3-1-4).

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The setting method is described below:1) Press [EDIT/EN TE R ] and the selected method wil l f lash.

{Example} CR FILTER ( f lashing)2) Use the [A ]/[V] keys to select the desired method .

{Example} CR FILTER ( f lashing) -> DIG ITA L FILTE R ( f lashing)3) Press [EDIT/EN TE R ] and the selected method wil l stop f lashing,indicating that it has been entered.

{Example} DIG ITA L FILTER

4-3-2 Method of useThe signal f i l ter method is normally set at "CR FILTER"; FST, STD, and SLW canbe selected depending on the peak width and degree of noise.If a high noise level makes analysis using the "CR FILTER" method diff icult,switch to the "DIGITAL FILTER" method.The DIGITAL FILTER method reduces baseline noise more than the CR FILTERmethod.The CR FILTER response speed when set at STD is approximately equivalent tothe speed of the DIGITAL FILTER set at 3S; and the CR FILTER response speedwhen set at SLW is approximately equivalent to that of the DIG ITA L FILT ER set at5S.

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Operation Manual

Caution:(1 )

(2)

A period of approximately three times the set value is requiredbefore the effect of the digital filter is seen. For example, whenthe sett ing value is 5S, about 15 seconds are required toprocess the signal.If a parameter were changed ( i .e. wavelength or gain) no effectwould be seen for about 15 seconds in the above exam ple.It is therefore advisable to avoid changing parametersimmediately before a peak.Due to the operating principle of the digital filter, signals aredelayed by approximately 1.5 times the setting value. Forexample, when the setting value is 5S, the peak elution time willbe delayed by about 8 seconds. Whe n detecting peaks using theintegrator, it is therefore necessary to keep the setting valuesconstant.

Setting value

3S5S

10S20S40S

Approximate signalprocessing time(seconds)

8153060

120

Approximate delaytime (seconds)

48

153060

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Operation Manual

4-4 Autozero operation ([SHIFT][4] setting)This section describes the use of the autozero function

4-4-1 Operation methodW hen the parameters shown below are changed, this screen can be used toselect whether or not to autozero the fluorescence signal.When set to "AUTO", the autozero will occur immediately after the parameterchange.W he n set to "MAN UA L", no autozero wil l occur.

-Whe n changing wavelength (EX, EM)-When changing gain-When MARKER IN (on the back panel) is input-When changing spectrum bandwidth (see section 4-8)

Note: The [MA RKER] key function on the front panel adds a marker regardless ofthis setting.

4-4-2 Setting methodThe autozero operation method is set on this screen.

Autozero screen

[SHIFT] [ATTEN/4] [MONIT OR]

Monitor screen NORM 0 .0000 STD300 500 1000 16

Figure 4-4 Setting the A utozero Ope ration MethodJasco FP-920 29


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Operation Manual

T he se tt ing method is shown below:1) Press [EDIT/EN TE R ] and the selected operat ion method wil l f lash

{Example} MANUAL ( f lashing)

2) Select using the [A]/[V] keys{Example} MA N UA L ( f lashing) -> A UT O ( f lashing)

3) Press [EDIT/EN TE R ] and the selected method will stop f lashin g, indicat ingthat it has been entered{Example} AUTO

4-4-3 Me thod of useIt is convenient to use the AUTO sett ing for analysis and the MANUAL sett ing forseparat ion.

- Autozero is automatical ly executed when the wavelength or gain is changed

either manually or via a time program.- W he n performing repeated analyses using the autosampler, autozero isautomatical ly executed with the addit ion of a marker when the MARKER INsignal is input.

- Since no autozero is executed when the wavelength or gain is changed as apeak is being detected, the signal does not return to zero, and therefore,gives a more natural chromatogram.- Since no autoz ero is executed with the add ition of a m arker whe n theMARKER IN signal is input as a peak is being detected, the signal does notreturn to zero causing the chromatogram to h ave no steps.- W he n searching for the wavelength corresponding to a peak maximum

during peak detection, the wavelength is easy to f ind since autozero is notexecuted.

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Table4 -1 Autozero Operation Example

Operation Manual

^Chromatogramchange

tting

Wavelengthchange MARKER INinput

AUTO

MANUAL

AUTO

MANUAL

MARKER IN

MARKER IN

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Operation Manual

4-5 Integrator output zero point shift ([SHIF7][5] setting)T his section describes the procedure for shifting the integrator output zero point.

4-5-1 Setting methodThe approved input voltage for the integrator is in the range of -10mV to+1V. In other words, the dynamic range on the minus side is small comparedto that on the plus side. With the zero point shift function, the voltage isshifted beforehand to the plus side, thereby preventing the integrator outputfrom falling below -10mV.Shift quantity can be set at 0, 5, 10, 50, or 100mV.

Zero pointshift screen

[SHIFT][RSPNS/5]

Monitor Screen

A . Z POSITION(IN TE OUT) 50 mVINORM 0.0000 STD300 500 1000 16

[MONITOR]

Figure 4-5 Setting the Zero Point Shift

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T he sett ing method is shown below:

1) Press [EDIT/EN TE R ] and the selected number wil l f lash.{Exam ple} 0 mV (flashing)

2) Select using the [A]/[V] keys.{Examp le} 0 mV (flashing) -> 50 mV (flashing)

3) Press [EDIT/EN TE R ] and the number wil l stop f lashing.{Example} 50 mV

Note 1: Zero point shift does not apply to recorder output.

Note 2: The extent of integrator output noise is not changed by the zero pointshift setting. However, as the input voltage becomes larger, the signalresolution of the integrator becomes smaller and the visible baselinenoise becomes larger. Therefore, do not set values that are larger thannecessary.

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Operation Manual

4-5-2 Method of use{Example 1} The baseline has a negative drift over a long time period.

co+ JdLCD aC 3 o

AO V

1 0 m V

oC3

Q_c D / | \ o

OV

Z e r o pot t s hiftO m V

Zer o poin t shift+ 1O m V

Figure 4-6 Metho d of Using Zero Point Shift

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Operation Manual

4-6 R ecorder output polarity ([SHIFT ][6] setting)This section outlines the recorder output polarity.

4-6 -1 Setting methodT he p olarity of the recorder output (Note) is set on this screen.

Note: The output polarity change is not applied to integrator output.

Recorder outputpolarity screen POLARITY(R E COUT) +[SHIFT][A.Z/6]

Monitor screen

[M ON ITOR ]

NORM 0.0000 STD300 500 1000 16

Figure 4-8 Setting of R ecorder Output Polarity

The setting method is shown below:1) Press [EDIT /EN T ER ] and the polarity wil l f lash.{Example} + (flashing)2) Select the desired polarity using the [A]/[V] keys.{Example} + (flashing) -> - (flashing)

3) Press [EDIT/EN T ER ] and the polarity wil l stop flashing, indicating that it hasbeen entered.{Example} -

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Operation M anual

4-6-2 Method of use{Example} R ecording in parallel with another detector

FP Polarity: +

UV _ A A

FP

UV

Polarity: -

Figure 4-9 Method of Using Recorder Output Polarity

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Operation Manual

4-7 Xenon lamp use time ([SHIFT ][7] setting)The total time the lamp has been lit will be displayed on this screen, and is usedto determine w hen to replace the lamp.The life of a xenon lamp is about 1,000 hours; and even though their emissionmay still be good, lamps that have accumulated 1,000 hours of use should bereplaced, since the glass port ion of the lamp is fatigued and mechan ically weak.

Xenon lampuse t ime screenLAMP OPERATION

120.5 HOUR(S)

[SHIFT][EMSP./7]

Monitor screenNORM 0 .0000 STD300 500 1000 16

r [MON IT OR]

Figure 4-10 Displaying the Lamp Use T ime

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Operation Manual

4-8 Spectrum b andwidth ([SHIFT][9] setting)This section outlines the procedures for the setting and use of the spectrumbandwidth.

4-8-1 Setting methodThe fluorescence spectrum bandwidth is set on this screen.

Spectrumbandwidthscreen[SHIFT][SLIT/9]

Monitor screen

B A N DEMt

WID T H: 18

(nm)

I [MONITOR]

NORM 0.0000 STD300 500 1000 16

Figure 4-11 Setting the Spectrum B andw idth

The setting method is explained below:1) Press [EDIT/EN TER ] and the selected spectral bandwidth will f lash.

{Example} 18 (f lashing)2) Select the desired valu e using the [A ]/[V] keys.

{Example} [40] (f lashing) -> 40 (f lashing)3) Press [EDIT/ENTER] and the selected spectral bandwidth wil l stopf lashing, indicating that it has been entered.

{Example} 404) Slit switching will begin immediately and the message below will bedisplayed until the operation is f inished (16 seconds at most).

NOW SET T ING

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Operation Manual

Note: While the NO W SETT ING message is displayed, the integrator ou tput andrecorder output will be maintained at the values set imm ediately p rior to theslit switching operation. When switching is complete, operation will continueaccording to the autozero operation method settings (see section 4-4).

4-8-2 Method of useSensit ivity and wavelength select ivity can be improved by changing the spectrumbandwidth.Example 1: By narrowing the spectrum bandwidth w hen the emissionwavelength is close to the excitation wavelength, the excitation lightand scattered light can be decreased, thereby improving sensitivity.Example 2: Wh en a single component is suf f icient ly separated from other peaks,f luorescence can be strengthened and sensit ivi ty increased bywidening the spectrum bandwidth.

Ta ble 4-2 Using the Spectrum B andwidth Sett ings

Setting (nm)

101840

Use

Used to improve wavelength selectivityStandard setting valueUsed to enlarge the f luorescence signal

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Operation Manual

4-9 Program number ([SHIFT][PRGM] setting)The program number (0 - 9) is set on this screen. Set the program number whenoperat ing in the program mode.

Program number screen(No. 5 is set in this example)PROGRA M F ILE NO.

[SHIFT] [PRGM] tMonitor screen

NORM 0 .0000 STD300 500 1000 16

Program numberPRCProgram m odemonitor screen I [ P R G M ]

[MONIT OR]

R G 5 MON T :L 0 .0300 500 1000 16

Figure 4-12 Sett ing the Program N umber

The method for setting the program number is shown below:1) Press [EDIT/EN TE R ] and the selected program number wil l f lash .

{Example} 0 (f lashing)2) Input the desired value using the numeric keys, or select it using the [A ]/[V]keys.

{Examp le} [5] becomes -> 5 (f lashing)3) Press [EDIT/EN TE R ] and the number will stop f lashing, indicat ing that it has

been selected.{Example} 5

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Operation Manual

4-1 0 Wavelength range ([SHIFT][CLEAR] sett ing)T he EX and EM wavelength range is set on this screen.

Setting

EM > EX + 10

NOT REST R IC T ED

Wavelength range

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Operation Manual

T he method for sett ing the wavelength range is shown below:1) Press [EDIT/EN TE R ] and the selected wavelength range wil l f lash.

{Example} N OT REST R ICTED (f lashing)

2) Select the desired wav eleng th range using the [A ]/[V] keys.{Example} N OT R EST R ICTED ( f lashing) -> EM > EX + 100 (f lashing)

3) Press [EDIT/EN TE R ] and the selected value will stop f lashing, indicatingthat it has been selected.{Example} EM > E X + 100

Caution: When the wavelength range is set to NOT RES TRICTE D, a sett ingof EX = EM is poss ible. In this situation scattered light stronge rthan the fluorescent light is incident on the light detectorphotomultiplier tube. To prevent deterioration of thephotomultiplier tube caused by the strong light, preset the gain toa small value (1 or 10 ).

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Operation Manual

4-11 Temperature correction ([SHIFT][N IARKER] setting)The internal temperature of the instrument is displayed on this screen.

Note: This fluorescence detector senses the temperature inside the instrumentand suppresses temperaturedrift.

Temperaturecorrect ion

[SHIFT] [MARKER]

Monitor screen

T EMP.COF.

t44.91.07959731

1NORM 0 .0000 STD300 500 1000 16

It is normal forthe last 4 or 5digits to changein this display.

[ M O N I T O R ]

Figure 4-14 Displaying the Te mperature

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Operation Manual

5. Key Operations in the Program ModeThis section outlines the key operations in the program mode.

5-1 OutlineThe program mode is used to change the detect ion wavelength dependingon the elut ion t ime and to use the autozero funct ion dur ing an an alysis. T hefol lowing parameters can be programmed in the program m ode.-Wavelength (EX and EM)-Sensi tiv ity (GA IN and A TT EN )-Response speed (RESPONSE)-Autozero-Wavelength scan (EX and EM spectrum m easurement)-Spectrum bandwidth (EM)There are 10 program f i les, each of which can contain a 64 step program.Set the program number (see section 4-9), and then proceed to use theoperations described in this chapter.

5-2 Switch to program modeWhen the [PRGM] key is pressed while in the normal operat ion modescreen, the mode will change to the program mode. To return to the normaloperat ion mode from the program mode screen, press the [PRGM] keyagain.

N ormal operat ionmode m onitorscreenNORM 0.0000 STD300 500 1000 16

[PGRM]PR G 5 MON T : 0 .0300 500 1000 16 [MONIT OR]

PRG 5 0.0000 STD300 500 1000 16

Program m ode monitor screensFigure 5-1 Switch between N ormal Operat ion Mode and Program Mode

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Operation Manual

5-3 Program mode monitor screenT here a re 2 types of monitor screens in the program mode.Use the [MON ITOR ] key to change between screens 1 and 2.

1 A screen displaying the time, etc.2 A screen displaying the range, waveleng th, and f luorescen t intensity.

Program mode #5

P R G 5 ) M O N300 500 100tf

Current timeO.Omin

[MONIT OR]

Program mode #5 Fluorescentintensity 0.0000 R esponse speed (R ESPON SE)Standard

EX(nm) / \ EM(nm)

Excitat ion'wavelength300 nmEmissionwavelength500 nm

Gain1000 At tenuat ion16

Figure 5-2 Explana tion of the Program Mon itor Screens

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Operation Manual

5-4 Input method for initial program param etersWhen the monitor screen is displayed, press the [V] key to enter the initialparameter settings screen.

PRG5 MON T: 0.0300 500 1000 16 [MONITOR]

Monitor Screens

PRG 5 0.0000 STD300 500 1000 16

P5300

[V]ST

r0

500INIT1000

[A]STD16

*[MON IT]

Initial parameter settings screenFigure 5-3 Initial Parameter Settings Screen

T ime program mode Initial parameter (step 0) R esponse#5 \ I STD

Excitation wavelength Emission wavelength G AIN A TTEN300nm 500nm 1000 16

Figure 5-4 Explanation of the Initial Parameter Settings Screen

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T he parameters are set as shown below:

Operation Manual

Screen Explanation of key o perat ion

EDIT/ENTEF

KPKS5 W .LI W.L22 EDIT/ENTEF

VP 5 S T 0 I N I T S T D\ i '3 5 5 5 1 2 1 0 0 0 1 6' I ^

EDIVE N T E F

(press twice) (See note)Continued on next page

Sets the excitat ion wave length

Input 355nmOnce the excitat ion wavelength isset, the instrument automaticallyproceeds to the emission wa velengthsetting

Input 512nmOnce the emission wavelength isset, the instrument automaticallyproceeds to GAIN sett ing

Input 10

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Operation Manual

From previous page

E D I VE N T E F

(Press 4 times) (See note)

Once the G AIN is set, theinstrument automatically proceedsto AT TEN setting

Input 1Once the ATTEN is set, theinstrument automatically proceedsto the response setting

Input FST

Figure 5-5 Initial Parameters Input Method

Note: Thenumerickeys can be used as w ell as the[A]/[V\keys.

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Operation Manual

5-5 Input method for program step 1 and afterA n input example is shown in Fig. 5-6. The input procedure is described insections 5-5-1 through 5-5-3.Screen

n to step 1)

P 5 ST 0 I N I T F S T3 5 5 5 1 2 1 0 1

\ /A

P 5 S T 1 T : 1 . 0E X : 3 0 0 EM : 5 0 0

A\/

P 5 S T 2 . T : 2 . 0G A I N 1

\lA

P 5 S T 3 T : 3 . 0R E S P O N S E S T D

\tA

P 5 S T 4 T : 4 . 0A U T O Z E R O

\lA

P 5 S T 5 T : 5 . 07 W : 0 . 1 L : 5 . 0 M : 2

\lA

P 5 S T 6 T : 0 . 0

ExplanationInitial parameter setting screen

After 1 minute, the excitationwavelength is set to 300nm and theemission wavelength to 500nm

After 2 m inutes the G AIN is set to 1

After 3 minutes, the response is setto STD

After 4 minutes, the autozerooperation is performed

After 5 minutes, the fluorescencespectrum is measuredR efer to section 5-8 for details

Figure 5-6 A n Example of InputJasco FP-920 49


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Operation Manual

5-5-1 New input (used if step is left blank)The input descr ibed here fol lows the sequence used when there is no input instep 1 (ST 1) or later steps. A summary of the sequence is il lustrated below.1) Time setting2) Func tion setting3) Value setting4) Procee d to the next step5) R eturn to monitor screen after input is complete

Screen Explanation of key operation

P5 ST 03 5 5 5 1 2

\ 1 TP5 ST 1

\\ 1E D I VE N T E F

P5 ST 1

\ iW.LI1

P5 ST 1

I N I T10

STD1

T: 0.0

T: -

E D I T /E N T E J

T:N O . l EX , EM W

\ /V.L1

P5 ST 1EX : - 2 0 0 -

\ l /0 . 0 -' 1 N

1 .0 L

IST :

EM :1 .0480

Move from step 0 (ST 0)to step 1 (ST 1)Input number value(1 is input in this example)

Display time

Wa it ing for time input

Input number value(1 is input in this exam ple)Wa it ing for funct ion input

Display wavelength

Wait ing for excitat ion wavelengthinput

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From previous page

P 5 S T 1 T : 1 . 0\ l EX : - 2 0 0 - EM : 48 0

/ I s

CfilN\ /

END EN D0 EDIVENTEfP 5 S T 1 T : 1 . 0EX : 3 0 0 EM :-\ 6

R S P N S\ /

EN D0 EN D0 EDIVENTEfP 5 S T 1 T : 1 . 0E X : 3 0 0 EM : 5 0 0

\ /P S S T 2 T : 0 . 0

\ /E D I T /E N T E f

\ I /P 5 S T 2 T : - 0 . 0 -

W.L22 E D I VE N T E FP 5 S T 2 T : 2 . 0N 0 . 1 E X , E M W . L .

C f l I N\\ E D I VE N T E R

Continued on next page

Operation Manual

Wait ing for excitat ion wavelengthinputInput number value (300nm is inputin this example)Wait ing for emission wavelengthinputInput number value (500nm is inputin this example)

Move from step 1 (ST 1)to step 2 (ST 2)

Display time

Wa it ing for t ime input

Input number value(2 minutes is input in this example)

Wait ing for funct ion input

Display G AIN

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Operation Manual

From previous page

iP R G S T 2 T : - 2 . 0 -G A I N 10 I

W lEDIl\l (See note)

P 5 S T 2 T : 2 . 0G A I N 1

Wait ing for GAIN inputInput number value(1 is input in this example)

Note: The numeric keys can be used instead of the [V]/[A] k eys.

Figure 5-7 Input Method

Repeat the same key operations for input in step 3 and later.The method for select ing a sett ing value dif fers depending on the parameter.Refer to Table 5-1 for a list of these differences.Once input is complete, press the [MON ITOR ] key to return to the m onitor screen.The program will be arranged chronologically at this point.

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Table 5-1 Functions in the Program Mode Function

Function

WavelengthExcitation (EX)Emission (EM)GAIN

ATTEN

Response

AutozeroEM spectrummeasurement(see note)EX spectrummeasurement (%)(see note)Spectrumbandwidth

Setting key

[W.L1/1]

[GAIN/3]

[ATTEN/4]

[RSPNS/5]

[A.Z/6][EMSP./7]

[EXSP. /8]

[SLIT/9]

Keys used to change

Numeric keys

Numeric or[A]/[V] keysN umeric or[A]/[V] keys[A]/[V] keys

/Numeric keys

Numeric keys

Numeric or[A]/[V] keys

Setting range

200 - 900 (nm)

1,10, 100, 1000

S, 256, 128, ... 2, 1

FST, ST D, SLW3S, 5S,...40S/W: 0.0-9.9(min)L: 0.0 - 99.9 (%)M: 0 -9W: 0.0 - 9.9 (min)L: 0.0 - 99.9 (%)M: 0 -910,18, 40 (nm)

Note: For more detailednformation refer to section 5-8, Spectrum M easurementin the Program M ode.

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Operation Manual

5-5-2 Edit method (for chang ing time and parameters)The input sequence used when a program has already been input is describedhere. In this example, the desired correspondence between time and parametersettings differs from that already set. In the example in section 5-5-3, the desiredcorrespondence is the same and only the values are changed.

ScreenP 5 S T 1

7 W : 0 . 1 L

\ (P 5 S T 1

7 11 : 0 . 1 L

\ 1P 5 S T 1N o . 1 E X ,

IP 5 S T 1EX : - 3 5 5 -


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Operation Manual

5-5-3 Edit method (for changing only values)The input sequence when a program has already been input is described here. Inthis example, the desired correspondence between time and parameter settingsremains the same and only the values are changed. In section 5-5-2, the desiredcorrespondence also differs from that already set.

Screen Explanation of key operation

\

E N T E F

P 5 S T 1EX : 3 5 5 - EM

' I NT : 1 . 0

5 1 2

C A I N EN D, 0\lEN D0 EDIVENTLF

P 5EX :

\P 5EX :

ST 13 0 0

IST 13 0 0

T : 1 . 0EM :- 5 1 2

R S P N S5 EN D0

T : 1 . 0EM : 5 00

EN D0 EDI I /E N T E F

Display time

Waiting for time input

Since there is no need to change thetime setting, simply press[EDIT /ENTER]

Wait ing for excitation w avelengthinput

Number input (300nm is input in thisexample)

Wa it ing for emission w avelengthinputN umber input(500nm is input in this example)

Figure 5-9 Edit MethodJasco FP-920 55


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Operation M anual

5-6 Program editing methodThis section describes the program editing method.

5-6-1 Step insertionA new step can be added to the program. The program will be arrangedchronologically when returning to the monitor screen after input.1) Display a step with no parameter input2) Refer to section 5-5-1 and input parameters

Screen Explanation of key operationP R G 53

P 53 5 5

P 5E X:

P5

M O N5

\1S T0

5 1

\1S T13

\1S T2

G A I N

T : 01

TI N I T

2 10

ST : 1

E M : 500

T : 2

1

1 6

F S T1

0

0

iP 5 S T 5 T : 5 . 0

7 W : 0 . 1 L : 5 . 0 M : 2

(Move directly to last step)


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Operation Manual

From previous page

am mode

P 57 W :

P 5

ST 50 . 1

\ 1ST 6

\ (P5 ST 6

ATTEN

PRG53 0 0

\MON

5 0 0

T :L : 5 .

T:

T :2 5 6

ro wT :

1 0 0 0

50

0

3

0

. 0M : 2

. 0

. 5

016

R efer to section 5-5-1 and input

Input procedure is omitted

R eturn to monitor screen.A t this time, the correspondencebetween step number (ST n) andtime (T) will be arrangedchronologically.

Figure 5-10 Step Insertion Method

Table 5-2

ST n

123456

Arrangement after Step InsertionArrangementat time ofaddition

123453.5

Arrangementwhenreturning tomonitorscreen1233.545 T able arrangedchronologically.

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Operation Manual

5-6-2 Step deletionW he n 0 is input for time (T ), the step will be deleted.

1) Display the step to be deleted2) Input 0 as the time of that step

Screen Explanation of key operationPRG5 MON T :

3 0 0 5 0 0 1 0 0 0

\ /P5 ST 0 I N I T3 5 5 5 1 2 1 0

\ /P5 ST 1 T:EX : 3 0 0 EM :

\ / P5 S T 2 T :

GAIN 1

\ / E D I T /E N T E RP5 ST 2 T : -

GAIN 1

\o

/P5 S T 2 T :

RESPONSE ST D

0 . 016

FST1

1 . 05 0 0

2 . 0

s 1 /2.0 -' 1 N

E D I 1 / -E N T E f

3 . 0


Display step to be deleted

Input a time of 0 to delete the step

The program in step 3 will move upto step 2

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From previous pag e

Operation Manual

Program modemonitor screen

P5 . ST 2 T: 3 .0RESPON SE STD

\ /PRG5 MON T: 0.0

300 500 1000 16

R eturn to monitor screen

Figure 5-11 Step Deletion Method

T able 5-3 A rrangement af ter Step Delet ion

S T n

123445

Arrangementbeforedelet ion

12->03445

Arrangementwhenreturning tomonitorscreen

1343.55

Unnecessarystep is deleted.

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Operation M anual

5-6-3 Section deletion (delete the designated step and a ll steps thereafter)1) Display the first step of the series to be deleted2) Input [SHIFT][CLEAR]

ScreenP R G 5 MON T: 0 0

3 500 1000 16

P 5 ST 0 I N I T FST3 5 5 512 10 1

P 5 ST 1 T: 1 0EX : 300 EM : 500

\/P 5 ST 2 T: 2 0

G A I N 1

P 5 ST 3 T: 3 0R E S P O N S E STD

\/P 5 ST 3 T: 0 0

Explanation of key operation

Display the first step of the series tobe deleted (In this example step 3and after will be deleted.)

Steps 3 and after are deleted

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Operation Manual

From previous pageP 5 S T 3 T : 0 . 0

V

Steps 3 and above are deleted

R eturn to monitor screenPRG5 MO N T: 0.0

300 500 1000 16

Figure 5-12 Section Deletion Method

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Operation Manual

5-7 Program exampleA chromatogram to help explain the time program is shown in Fig. 5-13. The 5peaks are detected under the condit ions shown in Table 5-4. An example of atime program under these same conditions is then shown in Fig. 5-14.

Fluorescence intensity

A Time (min)t 1 0 20t 3 0 5 0EM W av elen gth 300 nm 370 nm 500 nm

A ttenua tion 32 16Response ST D SLOW

AutozeroFigure 5-13 Chrom atogram for T ime Program Explan ation

T able 5-4 Detection Conditions for Each Peaks \ C o n d i t i o n

Peak number *12345

EM W avelength(nm)

300370500samesame

Attenuation

32samesame162

Response

STDsamesameSLOWsame

Comments

Autozero justbefore peak

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P3250

ST 0300

I N I T10

STD32

InitialEXEMG A I NA T T E

condit ions: 250nm: 300nm: 10: 32RESPONSE: ST D

P 3E X :

P 3E X :

P 3

ST 12 5 0

ST 22 5 0

ST 3ATTEN

P 3 S T 4R E S P O N S E

P 3

P 3

ST 5ATTEN

ST 6AUTO

T : 8 . 0EM: 370

T : 2 0 . 0EM: 500

T : 2 7 . 016

T : 2 7 . 0SLW

T : 4 1 . 02

T : 4 4 . 0ZERO

Change EM wavelength to 370nmafter 8 minutes

Change EM wavelength to 500nmafter 20 minutes

Change A ttenuation to 16 after 27minutes

Change response to SLOW after27 minutes

Change Attenuation to 2 af ter 41minutes

Perform an autozero after 44minutes

Figure 5-14 Program Example

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Operation Manual

5-8 Spectrum measurement in the program modeT his section describes spectrum measurement in the program mode.

5-8-1 OutlineA wave length scan can be executed when the fluorescent intensity exceedsthe set level within a set time period during a time program execution.The spectrum obtained by the wavelength scan can be saved in memory andthen output in the wavelength scan mode.

Note: Scan range and Scan speed are automatically set in the program mode;refer to section7-7-2.

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5-8-2 Setting methodT he fol lowing 4 parameters are set for spectrum measurement.

1 Tim e (" T on the screen)2 A llowed time interval ("W" on the setting screen)3 T hresh old value for f luoresce nt intensity ("L" on the setting screen )4 Memory number where spectrum is saved ("M" on the screen)

The sett ing method is explained here using the chromatogram shown in Fig. 5-16as an example.

FluorescentIntensityA 100%--

70% --

0 7 . 4 Time (min)Figure 5-16 Chromatogram for Spectrum Measurement Explanation

The peak elution time for the spectrum to be measured is 7.4 minutes and thepeak height is 90% of the maximum f luorescent intensity.A setting example for spectrum measurement with regard to this chromatogram isshown in Fig. 5-17, the input method is shown in Fig. 5-18.

Note: The numeric value shown on the "SIGNAL" column of the LCD indicatesthe fluorescent intensity, and has amaximun value of (+)1.0000.

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Fluorescent speptrum indicator(T he num ber "8" is displayedwith the excitation spectrum) Peak elut ion t ime

A llowed t ime interval(W stands for window) Fluorescent intensitythreshold value(L stands for level)Memory number(M stands for memory)

Figure 5-17 Example of Fluorescent Spectrum Mea surem ent Settings

Explanations of the example settings in Fig. 5-17 are provided below:Minimum f luorescent intensity for measur ing spectrum : 70 % of maximumvalue (Th reshold value "L " of f luorescent inensity)The setting range of "L" is from 0 to 99.9. This value shows the minimumfluorescent intensity at which spectrum measurement will be performed, as aproportion of the maximum fluorescent intensity. In Fig. 5-17 for example, thesetting (L: 70.0) means that spectrum measurement will occur only atfluorescent intensities of (+)0.7000 and above.

T ime for mea suring spectrum ("T"): 7.4 minutesA llowe d time interval ("W"): 0.2 minutes (+0.1 minute)The setting range of "W " is from 0.0 to 9.9 (min). This is the interval w ithregard to time T over which the spectrum is measured. In other words, thespectrum will only be measured between T -W/2 and T + W/2.Mem ory number whe re spectrum is saved ("M"): 5The setting range of "M " is from 0 to 9.

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ScreenP 5 S T 3 T : 0 . 0

\ /EDIVENTEF

P 5 S T 3 T : - 0 . 0 -

EMSP.M fllTB"4 E D I VENTEfP 5 S T 3 T : 7 . 4N O 1 E X , E M W . L .

\ /EMSP. EDIT/ENTEF

P 5 S T 3 T : 7 . 4^ \ ,7 W : 0 . 0 L : 0 . 0 M : 0' I N

\ /EN D0 . W.L2 :DIVE N T E F

P RG S T 3 T : 7 . 4\ I /7 W : 0 . 2 L : - 0 . 0 - M : 0

\ /EMSP.7 EN D0 E D I VE N T E F

P R G S T 3 T : 7 . 4S P W : 0 . 2 L : 7 0 . 0 M : - 0 -' I

R S P K S

\ /E D I VE N T E F

P RG S T 3 T : 7 . 4S P W : 0 . 2 L : 7 0 . 0 M : 5

Operation Manual

Explanation of key operat ion

Set time

Input 7.4 minutes

Input 7 to set f luorescent spectrummeasurement

Input 0.2 minutes

Input 70%

Input 5

Figure 5-18 Input for Fluorescent Spectrum Me asure men tJasco FP-920 67


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5-8-3 Execution exampleWith the example settings shown in Fig. 5-17, when the fluorescent intensity is70% or more of the maximum value between 7.3 and 7.5 minutes, the spectrumwill be measured and saved in memory number 5. The spectrum will not bemeasured if any of the conditions is not met.

FluorescentIntensity

70%

The chromatogram is fixedduring spectrum measurement

0 5 7 . 4With spectrum measurement

T ime (min)

FluorescentIntensityA

70% --

0 5 7 . 4Without spectrum measurement

Figure 5-19 Chromatogram with and without Spectrum Measurement

Time (min)

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5-8-4 Spectrum outputThe spectrum is output from the wavelength scan mode. Refer tosection 7-5 for the detailed operation procedure.

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6. OperationT his chap ter out lines the operat ing procedures.

6-1 Operation in normal operation modeT his sect ion descr ibes op erat ion in the normal operat ion mod e.

6-1-1 Parameter sett ings.Refer to section 3-1 for an explanation of the parameter settings.

6-1-2 Error messagesThe error messages that may appear dur ing operat ion are shown inFig.6- 1. LAMP T IMER OFF however, is not an error (see sect ion 4-2).When an error message appears, press [CLEAR] to return to themonitor screen. If the lamp has been turned off by the LAMP OFFtimer, the power must be turned off then on again.

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N O R M 0 . 0 0 0 0 S T D3 0 0 5 0 0 1 0 0 0 1 6

AC L E t R

LAMPTIMER OFF

o r

T R O U B L EL E A K IN C E L L

Press [CLEAR] to return to themonitor screen

The LAMP OFF timer has turned thelamp off

There is a leak in the flowce ll

T R O U B L EE X D R I V E R E R R O R

orW A R N I N G

A U T O Z E R O

There is a problem in thewavelength driver section

Fluorescence (light emitted from theflowcell in general) is excessive

Figure 6-1 Possible Error Messages Displayed During Operation

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Operation M anual

6-1 -3 Measu res to take if the [AUT OZER O] key lamp is flashing or if the"WARNING AUTOZERO" message is displayed.When the intensity of l ight emitted from the flowcell is excessive, "WARNINGAUTOZERO" wi l l be displayed on the LCD, or the [AUTOZERO] key lamp wi l lf lash.In addition to high fluorescent intensity, causes of excessive l ight emission fromthe flowcell include excessive scattered l ight from the solvent, sample or from airbubbles in the flowce ll. Countermeasures are shown in T able 6 -1 .

T able 6-1 Countermeasures for Excessive Light Emitted from the F lowcell

Cause

Fluorescent intensity is highScattered l ight from thesolvent or sampleScattered l ight causedby air bubbles

Countermeasure

Decrease the "GA IN "Check solventCheck detected W avelengthRemove the air bubbles

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Operation Manual

6-2 Operation in the program modeT his sect ion descr ibes operat ing procedures in the program m ode

6-2-1 Init ial se tt ing sInitial settings

POWER ON

\lF P - 9 2 0JASCO V e r 1 . x x

B egin self -check

N O R M 0.0000 S TD300 500 1000 16

VP R O G R A M F I L ENO

5

VN O R M 0.0000 S TD300 500 1000 16

PRG 5 M0N T: 0.0300 500 1000 16Continued on next page

End self-check

Select program num ber(Only necessary when using anumber different from the programnumber last set when the power wasturned "OFF")

To program mode

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Operation Manual

Continued from previous page

PRG300

P5355

5

ST

MON500

\/0512

1000

I N I T10

0.016

FST1 Program creation

P R G 5 M O N T : 0 . 03 0 0 5 0 0 1 0 0 0 1 6 To program monitor screen

Note: When [PRGM ] is pressed, the status of the instrument does not changeeven though the mode displayed on the screen has changed. For example,when the mode is changed from NORMAL OPERATION to TIMEPROGRAM, the sensitivity and wavelength values set in the NORMALO PERATIO N m ode are stil l in effect, ( i.e., the initial conditions set in theTIME PROGRAM are not recognized). Use the following steps to changethe instrument settings to the initial conditions.(1) Set the sensitivity and wavelength parameters using the TIM E PRO GRAMmode MONITOR display.(2) Press [PRGM/RUN] once to advance the program proceed, then press itagain to stop the program.

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Operation Manual

6-2-2 Starting the program

( Start the program

Wa it for stable baseline inprogram monitor screen

[PRGM/RUN]Start TIME PR OGR AM

A small lamp lit on the top left cornerof the [PRGM/RUN] Key indicatesthat the program is in progress)[PRGM/RUN]

Figure 6-3 Procedure for Starting a Time Program

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Operation Manual

6-2-3 Changing parameters during a program runThe values set for attenuation,gain,response, wavelength, and lamp off timer canbe changed during program excecution. These changes can be made in thedisplay screens shown boxed with double lines. The old settings a re valid until theprogram progresses and new values are incorporated.

Setting changes during program execution

PR G 5 MON T: 10.1

300 500 1000 16

P R G 5 0.0008 FS T

300 500 1000 16

Lapsed time, w avelength,and range display screen

W . L I1

W.L2l j

Fluorescent intensity,response, display screen A

LAMP OFF TIMER

2 . 0 HOUR(.S)

Lamp off timer screen

E X : 2 . 6 0 3 V

E M : 1 . 8 6 5 V

r W N I T

Preamplified output screen

Figure 6-4 Changing Parameters During Program Execution76 Jasco FP-920


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Operation Manual

6-2-4 Stopping the programThe program can be stopped at any time during execution by pressing [PRGMRUN].Once a time program has reached the final step, the detector will continueto operate under the conditions of that step while time lapse alone progresses,until [PRG M RUN ] is pressed.

Stopping the program

During program execution

[PRGM/RUN]Stop program

The key lamp w ill go out, indicatingthat the program has stopped [PRGM/RUN]

Figure 6-5 Stopping a Time Program

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Operation Manual

7 Wavelength Scan ModeThis chapter outlinesthe use of thewavelength scan mode.

7-1 OutlineoffunctionsThe funct ionsof thewavelength scan modearel isted be low:-Spectrum measurement-Spectrum measurement parameter settings-Spectrum output-Spectrum dif ference outputWh e naspectrum is measured,theresultsarestored in memory. Thereare10 memory locationsfor storingEX and EMspectra.Thewavelength rangeover whichthespectrum wil lbemeasuredand other parameters can be setin detail,and the measured spectrum can be output to the integrator andrecorder.The spectrum dif ferencescan beoutput inaddit ionto the EX andEM spectra.

7-2 OutlineofoperationWhen enteringthe wavelength scan mode,the EMspectrum measurementwill f irstbedisplayedas a menu. Displaythefunctionsto be executed usingthe [A]/[V] keysordirectly us ingthenumeric keys. Toexecutethe d isplayedfunctions, press the [EDIT /ENTER] key and perform the key operat ionsaccordingto theguide messages.

Number1234560

Tab le7-1DisplayEM SPECTEX SPECTEMD.OUTEX D.OUTDIFFER EMDIFFER EXS C A N P A R A M

Wavelength Scanning MenuFunctionEM spectrum measurementEX spectrum measurementEM spectrum outputEX spectrum outputEM spectrum dif ference outputEX spectrum dif ference outputSpectrum measurement conditions

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7-3 N otes for use(1) Press the [MONITOR] key repeatedly to exit the wavelength scan mode.(2) Spectrum measurements can be performed using the wavelength scan modedescribed in this chapter, or using the time program. T he wave length scanmode has the ability to measure a spectrum under specif ied measurement

conditions, whereas the time program offers the advantage of automaticallymeasuring multiple spectra.(3) Spectrum output is performed in the wavelength sca n mode regardless ofwhether the spectrum was measured in the wavelength scan mode or the timeprogram.(4) Storage memory numbers 0 to 9 can be designated when measuring emissionspectra or excitation spectra, thereby allowing 10 spectra to be saved. Beaware that when choosing memory numbers two types of spectra can besaved using the same number. For example, the emission spectrum output #5and the excitation spectrum output #5 are completely dif ferent even thoughtheir numbers areident ica l.

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7-4 Spectrum measurementT he procedures for spectrum measurement are explained in this section.Since the procedures for both the EM and EX methods are the same, only EMspectrum measurement is used as an example.

N ormal operation mode T ime program modeL . . - - . _ - - _ - - - - - _ - _ - , _ _ _ - _ _ ,

S P E C T R U M M E A S U R EN O . 1 EMS P E C T

E D I VE N T H

E M S P E C T M E A S U R ES E T M E M O R Y N 0 . 0

\W L l l J E O I VE N T E f

E M S P E C T R U M ,\I /I N P U T E X : 5 0 0

COIN END0\(

E M S P E C T . M E A S U R EP U S H P R G M R UN

E M S P E C T . M E A S U R ER U N N I N G

\(

Wavelength Scanning Menu

Note: To measu re EX spectrumpress[W L2>2][EDIT/ENTER]

Select memory #1

END E D I VE N T E F Set the EX wavelength(300nm in this example)

Waiting for Scan

Execute scan

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7-5 Spectrum outputSpectrum output is described in this section. Since the procedures for both theEM and EX methods are the same, only EM spectrum output is used as anexample.

7-5-1 Key operations

Normal operation mode Time program mode

\ /SPECTRUM MEASUREN O . 1 E M SPECT

\[ G A I N 3 ] (o rp ress [V ] twice) nowifl

SPECTRUM MEASUREN o . 3 EM D.OUT

\l E D I VE N T E fEM SPE CT. NO. -l~

EX : 3 5 0 nm

vW.L22

EM SP EC T. N O.. - ^ 2 ^ -EX : 3 8 0 n m

V

Select memory #2

Display EX of memory #2

E D I VE N T U


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AContinued from previous page

\tE M S P E C T . EX : 3 8 0s IS T R T : - 3 9 0 - END: 760

9 T T E N4 E N D ]0 END0 E O l t /E N T E F

\lSetthe EMwavelengthatwh ichtostart output (400nm in this example)

EM SP E CT . EX : 38 0S T R T : 400 END: 7 6 0

T fl 26 S\JDo

E M S P E C T . D A T A 0 U TP U S H P R G MRUN

E M S P E C T .EX : 200E M : 461 S: 0.013

\l

ENDo

E D I VE N T E F

Se tthe EMwavelengthatwhichtostop output(600nminthis exam ple)

Wait ingforoutput

Execute outputDisplayEMwavelengthandf luorescent intensity

Returnsto base screen afteroutputhasf in ished

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7-5-2 Outpu t formThe spectrum is sent to the integrator output and recorder output. The recorderoutput intensity can be set using "AT T EN " (see "N ormal O peration" for settingprocedure). Wavelength markers will be added to the recorder output at 100nmintervals (ie: 200, 300..., 700, 800nm).The speed of the spectrum output is 100nm/30sec (about 3.3nm/sec), thereforewith a chart speed of 100mm/min, a 100nm spectrum will yield a chart 50mm long.

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Operation Manual

7-6 Difference spectrum outputDifference spectrum output is described in this section. Since the procedures inboth the EM and EX methods are the same, only EM spectrum output is outlined.

Note: As il lustrated n the following exam ple, the EXwavelengthof both spectramust be the sam e in order to output a difference spectrum. An errormessag e appears if the EX of the spec tradiffer. Similarly, the EM of thespectra must also be the same upon EXspectrumoutput.

7-6-1 Key operations

N ormal operation mode Time program mode

VS P E C T R U M M E A S U R EN O . 1 E M S P E C T

[RSPNS 5](or press [V \4 times) n o w n\lS P E C T R U M M E A S U R EN o . 5 D I F F E R E M

E D I Vi / E N T E J

S U B . 3 F R O M 2E X : 500 nm

T E N


Designate the spectrum of memorylocation #4

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R 3 > N S5 R 3 > N S5

V

EMSK71 ENDi0 I

S U B . - 4 - FROM 2 I sEX : 4 0 0 nra

\ 1 E D I T /S U B . 4 FROM - 2

EX : 5 0 0 nm

\ iW . L 11

S U B . - 4 F R O M ^ lEX : 4 0 0 nm

\\E D I VE N T E f

/_

/

D . EM EX : 4 0 0S T R T : - 5 1 0 - E N D : 6 5 0

T he EX wavelength when the EMspectrum of memory location #4 wasmeasured will be displayed on thelower part of the LC D.

Designate the spectrum of memorylocation # 1The EX wavelength when the EMspectrum of memory location #1 wasmeasured will be displayed on thelower part of the LC D.In this example, "Spectrum # 1 " -"Spectrum #4" wil l be output

EN Do EDIV;E N T E fSet the EM wavelength at which tostart output(550nm in this example)

D.S T R T :

EM55 0

EX :E N D :

400s I '- 6 5 0 -' I s

EN Do E D I VENTER\ /

Continued on fol lowing page

Set the EM wavelength at which tostop output(700nm in this example)

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D . EM D A T A O U TP U S H P R G M R UN

D . EM EX : 400E M : 553 S : 0 . 7 0 7 7

Waiting for output

Execute outputDisplay EM w avelength an d thedif ference in EM spectra intensites

Returns to base screen afteroutput has f inished

7-6-2 Output formSince the output form is exactly the same as the spectrum output, refer to section7-5-2.

7-6-3 Method of useIn addition to obtaining dif ference spectra for dif ferent samples, the dif ferencespectrum is also used to eliminate the inf luence of emission and scattering fromthe mo bile phase solvent itself.86 Jasco FP-920


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7-7 Spectrum measurement parameter settingsB oth the wavelength scan range and the speed for spectrum measurement can beset.

7-7-1 Key operationsN ormal operation mode Time program mode

\S P E C T R U M M E A S U R EN O . 1 E M S P E C T

V [EN D/0](or press [V] 6 times)now

S P E C T R U M M E A S U R ENO . 0 S C A N P A R A M .

VE D I VE N T E F

SCAN PARAMETERSRNG: STD SP D: 1

\lEDIV

SCAN PARAMETERS\ I /R NG: - S T D - S P D : 1

\l A EDIVENTEfS C A N P A R A M E T E R S

R N G : W I D E S P D :-l-

VContinued o nnext page

Set scan range to "WIDE"

Set scan speed to 2

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A iS C A N P A R A M E T E R S

R N G :W I D E SPD : 2

V Returns to base screen

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7-7-2 SettingsSettings and their corresponding descriptions are listed in the tab les below.

T able 7-1 EM Wavelength Scan RangeSettingWIDEST DNARROW

Wave length scan rangeOnm -> 900nmEX + 10nm->900nmEX + 10nm->2XEXnm(or 650 nm, whichever isshorter)

For example, the EM wavelength scan range is 260nm -> 500nm for theN AR R OW setting at EX 250nm.

T able 7-2 EX Wavelength Scan RangeSettingWIDEST DNARROW

Wavelength scan rangeOnm -> 900nm200nm -> EM -10 nmEM/2-> EM-10(or 220nm, whichever islonger)

T able 7-3 Wavelength Scan SpeedSetting

12

Wavelength scan range10Onm/sec30nm/sec

Note: Scan range and speed a re autom atically set to NARR O W and 1,respectively, when using the time program spectrum measurem ent.

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8. Specifications and OptionsThis section outlines the specif ications and options of the instrument.

8-1 SpecificationsMode l name: Model FP-920 Intelligent Fluoresce nce DetectorOptical system:Monochroma tor: Holographic concav e diffraction grating (for Ex

and Em)Light source: 150W xenon lamp (mounted horizontally)Wa velength range: 220nm - 650nm ( for EX and EM)

(Setting range: Onm - 900nm)Spectrum bandw idth: Excitation monoch romator: 18nmEmission monochrom ator: 10, 18, 40nm(can be switched by keypad input)

Wa veleng th accuracy: +2nmWa veleng th repeatability: +0.3nmDetectors: Excitation monoch romator: Pho todiode ,

Emission monoch ramator: Photom ultiplierFlow cell Capa city: 16jal (standa rd)Solvent wetted materials: Synthetic silica, f luorore sin, PEEK ,

stainless steel SUS-316Control system:Sensit ivity: S/N for the R AM AN peak of water is200 or g reater;where Ex = 350nm and thetime constant is 1.5 secondsMeasu rement range: 10 steps in total:

1,2, 4, 8, 16, 32, 64, 12 8, 256 , and SGain : x 1000, x 100, x 10, x 1Response : FAST, STA NDA RD, SLOW,

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Signal processing:

Output:

Input:

Program funct ions:

Operation Manual

Digital processing by A/D and D/A converters(with ambient temperaturecompensation circuits)R ecorder output: 10mV/FS(polarity change is possible)I ntegra tor outpu t: 1V/F SOne circuit each for marker and leak outputOne circuit each for marker and autozero inputProgram reset run inputT ime program possible with Ex wavelength,EM wavelength,gain, attenuation,wavelength scan, etc.

Wavelength scan functions: Excitat ion spectrum and emission spectrummeasurement (Manual and t ime program)Spectrum storage (10 excitat ion and 10emission spectra) and spectrum(difference spectrum) output

Sel f-check funct ion:

Lamp off t imer:Lamp use t imecalculation funct ion:Others:Dimensions and weight :Required power:

Am bient Temperature:

Memory (RO M and RA M), DC power, Ex energydecrease, leak incell, lamp use t ime99.9 hour maximum (0.1 hour increments).Internal timer for recording the total num ber ofhours that the Xe lamp has been used.

300(W) x 150(H) x 460mm(D), about 19kg11 5 V A C230 V A C selectable50/60HZ, maximum 300 W+10 to 35C for operation- 30 to 60C for storage

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8-2 Options (Optional accessories)Photomu ltiplier: High Sensitivity (200 - 700nm)Long wavelength (220 - 900nm)Flowce ll: High capacity (50^1)Micro-capacity (5jil)Square cell holder: For 10mm square cellsSecondary light filter: For excitation or emission

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9. Optional AccessoriesT his section describes optional accessories for the unit.

9-1 Filter types and usesThe Model FP-920 Fluorescence Detector is equipped with high efficiencydual monochrometer optics which make it unnecessary to use filters duringnormal measurement. However, when measuring emission at wavelengthsclose to 2 or 3 times the excitation wavelength, a filter is necessary toremove the second and third order light from the excitation wavelength. If theemission wavelength is set at 2 or 3 times the excitation wavelength, thesecond and third order light of the excitation light will stray into the emissionmonochrometer and become incident on the detector along with the lightemission of the sample. This creates a high background and noise, makinggood analytical results impossible to obtain.For example, with an excitation wavelength of 250nm, if the emissionwavelength is set at 500nm, the secondary light of the 250nm excitationwavelength (500nm) that has been scattered by the cell will also becontained in the 500nm emission wavelength. To remove this secondarylight, a UV-32, UV-36, L-39 or similar filter (the filter shape depends on theshape of the flow cell cassette) (see fig 9-4 and table 9-2), which cuts 250nmlight and allows 500nm light to pass, should be attached at the emissionoutlet of the flow cell cassette.

T able 9-1 Characteristics of Filters for the Model FP-920 Fluorescence DetectorTypeUV-D36CC-39BB-460UV-32UV-36L-39Y-46

UseExcitationExcitationExcitationEmissionEmissionEmissionEmission

CharacteristicsBand pass filter transmits light near 360nmBand pass filter transmits light near 390nmBand pass filter transmits light near 460nmSharp cut low pass filter, 50% transmittance320nmSharp cut low pass filter, 50% transmittance360nmSharp cut low pass filter, 50% transmittance390nmSharp cut low pass filter, 50% transmittance460nm

nearnear

nearnear

The spectral characteristics of the filters above are shown in Figs. 9-1 and 9-2.Jasco FP-920


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100

CD

ra

50

B-460

UV-D36Cj J \\ \c-39B\ B - 4 6 O

\ |

200 300 400 500 600 700

Wavelength (nm)Figure 9-1 Characteristics of Excitation Filters

100

831toI 50

UV-32

OV-36ifI 1r i Y-46^200 300 400 500 600 700

Wave length (nm)Figure 9-2 Characteristics of Emission Filters

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9-2 Square cell holderA 10mm square cell holder is designed to permit the Model FP-920 FluorescenceDete

Documents

Jasco Fp920 Manual 1995 Eng