May24,2018

IntroductiontoGenomics

Malvika Pillai

• WhatisGenomics?• GenomeSequencing• Genotyping

OutlineforIntroductiontoGenomics

WhatisGenomics?

Collection ofinformation

Subsetofinformationtobeexpressed

Problem

DefinitionofGenomics

Geno

mics

Functional

Comparative

Structural

etc.

• Large-scalestudyofgroupsofgeneswithinoneorganismorbetweenmultipleorganisms

GenomeSequencing

>NC_000017.11:c43125483-43044295 Homosapienschromosome17,GRCh38.p12PrimaryAssemblyGTACCTTGATTTCGTATTCTGAGAGGCTGCTGCTTAGCGGTAGCCCCTTGGTTTCCGTGGCAACGGAAAAGCGCGGGAATTACAGATAAATTAAAACTGCGACTGCGCGGCGTGAGCTCGCTGAGACTTCCTGGACGGGGGACAGGCTGTGGGGTTTCTCAGATAACTGGGCCCCTGCGCTCAGGAGGCCTTCACCCTCTGCTCTGGGTAAAGGTAGTAGAGTCCCGGGAAAGGGACAGGGGGCCCAAGTGATGCTCTGGGGTACTGGCGTGGGAGAGTGGATTTCCGAAGCTGACAGATGGGTATTCTTTGACGGGGGGTAGGGGCGGAACCTGAGAGGCGTAAGGCGTTGTGAACCCTGGGGAGGGGGGCAGTTTGTAGGTCGCGAGGGAAGCGCTGAGGATCAGGAAGGGGGCACTGAGTGTCCGTGGGGGAATCCTCGTGATAGGAACTGGAATATGCCTTGAGGGGGACACTATGTCTTTAAAAACGTCGGCTGGTCATGAGGTCAGGAGTTCCAGACCAGCCTGACCAACGTGGTGAAACTCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGGTGCCGCTCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTAGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCGCGCCATTGCACTCCAGCCTGGGCGACAGAGCGAGACTGTCTCAAAACAAAACAAAACAAAACAAAACAAAAAACACCGGCTGGTATGTATGAGAGGATGGGACCTTGTGGAAGAAGAGGTGCCAGGAATATGTCTGGGAAGGGGAGGAGACAGGATTTTGTGGGAGGGAGAACTTAAGAACTGGATCCATTTGCGCCATTGAGAAAGCGCAAGAGGGAAGTAGAGGAGCGTCAGTAGTAACAGATGCTGCCGGCAGGGATGTGCTTGAGGAGGATCCAGAGATGAGAGCAGGTCACTGGGAAAGGTTAGGGGCGGGGAGGCCTTGATTGGTGTTGGTTTGGTCGTTGTTGATTTTGGTTTTATGCAAGAAAAAGAAAACAACCAGAAACATTGGAGAAAGCTAAGGCTACCACCACCTACCCGGTCAGTCACTCCTCTGTAGCTTTCTCTTTCTT

Whatisagenome?

• Sangersequencing• NextGenerationSequencing(NGS)– IlluminaSequencingbySynthesis

Howdowesequenceagenome?

Genotyping

Genotyping

AnyQuestions?

IntroductiontoGenomics:Demo+Hands-OnExercise

NextClass

• MutatingRNAinMatlab– VisualizingRNAfolds– Comparingmutatedstrandstowildtypestrands

Demo