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From time to time, an area of scientificendeavour will become such a focalpoint of attention that it warrantsspecial treatment in the literature.Trends Guides are a unique serviceto our readers aimed at addressingthese emerging information needs.
Trends Guides are supplementarypublications produced in associationwith one or more Trends journals. Eachguide focuses on a topical subjectarea, often touching on fields that arenot covered extensively or exclusivelyby any one Trends journal.
Proteomics is one such topic thathas moved rapidly to the forefront ofscientific thinking, with interestspanning traditional life scienceboundaries. We are indebted to ourGuest Editors Walter Blackstockand Matthias Mann, and to all theauthors who have worked extremelyhard over recent months to ensure thatProteomics: A Trends Guide is asinformative and timely as possible.
Proteomics: A Trends Guide isbeing circulated with the August issuesof Trends in Biotechnology, Trends inBiochemical Sciences, Trends inGenetics, Trends in Microbiology andDrug Discovery Today.
We hope that you enjoy reading thissupplement and welcome yourfeedback and ideas.
Rachel WoodManaging [email protected]
1 Editorial – Proteomics comes of age: the quest for functionby Walter Blackstock and Matthias Mann
3 Advances in 2D gel techniquesby Angelika Görg
7 Experimentalism in proteomicsby Jeffrey D. Thomas and Ruth A. VanBogelen
12 Trends in automation and mass spectrometry for proteomicsby Walter Blackstock
17 Databases and knowledge resources for proteomics researchby Matthew E. Crawford, Michael E. Cusick and James I. Garrels
22 Database searching with mass-spectrometric informationby Ronald C. Beavis and David Fenyö
27 New methods of proteome analysis: multidimensional chromatography and mass spectrometryby Michael P. Washburn and John R. Yates, III
31 Using mass spectrometry for quantitative proteomicsby Steven P. Gygi and Ruedi Aebersold
36 Modification-specific proteomics: systematic strategies foranalysing post-translationally modified proteinsby Ole Nørregaard Jensen
43 Protein-interaction mapping for functional proteomicsby Ole Vorm, Angus King, Keiryn L. Bennett, Thomas Leber and Matthias Mann
47 Protein arrays: a high-throughput solution for proteomics research?by Dolores J. Cahill
XII Company profileOxford Glycosciences
XIV Company profileProteome Systems
Cover imageThe cover image depicts a multi-function tool from aproteomics workstation built to the specifications of theCell Map Project, Glaxo Wellcome, by KayeBeeEngineering, Basildon. The unit incorporates imaging,gel-cutting, bar code reading and nanolitre volume liquiddispensing, and cuts at up to 1020 gel cores per hour.
Editors Walter Blackstock and Matthias Mann Assistant Editor John Pettigrew Editorial Assistant Joanna Milburn Production Mick Ayres Managing Editor Rachel WoodPublishing Manager Rob Brines
Contents
Proteomics: A Trends Guide
-PROTEOMICS supp. [final] 14/7/00 11:59 am Page V