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Introducing …Introducing …The World’s Most Sensitive The World’s Most Sensitive
Ion Trap!Ion Trap!
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Universal Source …Universal Source …
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The Ion Max AdvantageThe Ion Max AdvantageReduces chemical noise No accumulation of API exhaust within chamberImproves S/N!
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2D Linear Trap Instrument
400 L/sec300 L/sec
Detector1
Source
Tube Lens
Skimmer
SquareQuadrupole Octopole
BackLens
BackSection
FrontSection
Ion TransferTube 3x10-3
Torr HeInter-multipole
Lens 1
CenterSection
Detector2
FrontLens
Quadrupole
8.3 L/sec 25 L/sec
Ion TransferTube
Square
Tube Lens
Electrospray
Split Gate Lens
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LTQ Top Cover Plate – Ion Optics
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LTQ Top Cover Plate – Ion Optics
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LTQ Vacuum Chamber – Linear Trap and Dynodes
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LTQ Vacuum Chamber with Top CoverView from Back – Dual Detectors
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LTQ Vacuum Chamber – Linear Trap and Dynodes
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LTQ Performance LTQ Performance
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5 fmol5 fmol Myoglobin Digest On LTQMyoglobin Digest On LTQC:\Xcalibur\data\Mar03\032003_14 03/20/2003 06:58:44 PM 1.1e-1/7.0e-4/80thou offset (repeat)500um cap, 2pum ps, 3.75 m m theo skim m ing dis t
RT: 0.00 - 30.01 SM: 7G
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30Tim e (m in)
0
10
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30
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100
Rel
ativ
e Ab
unda
nce
537.15425.46
432.71
461.18
473.01462.75
NL:4.71E7Base Peak F: + c ESI Full m s [ 400.00-1200.00] MS 032003_14
032003_14 #3305 RT: 9.46 AV: 1 NL: 3.70E7F: + c ESI Full m s [ 400.00-1200.00]
400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200m /z
0
10
20
30
40
50
60
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90
100
Rel
ativ
e Ab
unda
nce
425.4
637.2
C:\Xcalibur\data\Mar03\032003_14 03/20/2003 06:58:44 PM 1.1e-1/7.0e-4/80thou offset (repeat)500um cap, 2pum ps, 3.75 m m theo skim m ing dis t
RT: 0.00 - 30.01 SM: 7G
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30Tim e (m in)
0
10
20
30
40
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90
100
Rel
ativ
e Ab
unda
nce
537.15425.46
432.71
461.18
473.01462.75
NL:4.71E7Base Peak F: + c ESI Full m s [ 400.00-1200.00] MS 032003_14
032003_14 #3305 RT: 9.46 AV: 1 NL: 3.70E7F: + c ESI Full m s [ 400.00-1200.00]
400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200m /z
0
10
20
30
40
50
60
70
80
90
100
Rel
ativ
e Ab
unda
nce
425.4
637.2
150um column, 10um tip
Spectral S/N >> Spectral S/N >> 100!!100!!
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Improve Your Routine SensitivityImprove Your Routine SensitivityRT: 4.07 - 19.12
5 6 7 8 9 10 11 12 13 14 15 16 17 18 19Time (min)
10
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60
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80
90
100
10
20
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100
Rel
ativ
e Ab
unda
nce
908.7
925.4
760.5
908.6 629.5490.9767.1972.6636.0590.8 650.1
804.3 690.3771.1774.5 690.3852.3 462.1748.6 953.3435.9 537.6636.6 436.4567.4 462.0
536.7
629.5
909.0460.8
460.6424.9
NL:1.23E7Base Peak F: + c NSI Full ms [ 400.00-1000.00] MS 011003_08
NL:7.85E6Base Peak F: + c ESI Full ms [ 400.00-1000.00] MS myoglobin_02
1 fmol 1 fmol myoglobinmyoglobin digest injected (150um ID column)digest injected (150um ID column)
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Improve Your Routine SensitivityImprove Your Routine Sensitivity1 fmol 1 fmol myoglobinmyoglobin digest injected (150um ID column)digest injected (150um ID column)
\\re-server\tomcat\...\xpplus\011003_08 1/10/2003 3:58:51 PM1 fmol
011003_08 # 2326 R T : 11.13 A V: 1 N L: 3 .72E 6F : + c N S I F u ll m s [ 400 .00 -1000 .00 ]
400 500 600 700 800 900 1000m /z
0
10
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100
Rel
ativ
e Ab
unda
nce
536.7
804 .6
537 .7528 .8 895 .3632 .3 767 .4461.9 827 .7726 .1572 .2493 .6 639 .9 836 .0
938 .1 961.0
011003_08 # 3607 R T : 17 .23 A V: 1 N L: 6 .74E 6F : + c N S I F ull m s [ 400 .00 -1000 .00]
400 500 600 700 800 900 1000m /z
0
10
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Rel
ativ
e Ab
unda
nce
629.5
944 .0
943 .1
536 .5989 .6606 .4
532 .0 669 .4 816 .5561.2 724 .4486 .8 767 .2 939 .5879 .8696 .3457 .1
m yo glo bin_02 # 3378 R T : 11.74 A V: 1 N L: 7 .85E 4F : + c E S I F ull m s [ 400 .00 -1000 .00]
400 500 600 700 800 900 1000m /z
0
10
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Rel
ativ
e Ab
unda
nce
536 .7
m yo glo bin_02 # 5229 R T : 17 .61 A V: 1 N L: 3 .92E 4F : + c E S I F ull m s [ 400 .00-1000 .00]
400 500 600 700 800 900 1000m /z
0
10
20
30
40
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100
Rel
ativ
e Ab
unda
nce
629.5
472 .4
485 .6 943 .7536 .0 647 .0461.8 597 .5 665 .5 701.1 856 .4765 .5 879 .5 994 .6
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0.00
1.002.00
3.004.00
5.00
6.007.00
8.009.00
10.00
Run 1 Run 2 Run 3 Run 4Prot
eins
IDConsistent ResultsConsistent Results
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0
20
40
60
80
100
120
Tot
al N
umbe
r of
Yea
stPr
otei
ns (C
lust
ers
Top 3 Top 5 Top 7 Top 10MS/MS Scan Events
Proteins (LCQ)Clusters (LCQ)
0
50
100
150
200
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300
Tot
al N
umbe
r of
Yea
stPr
otei
ns/C
lust
ers
Top 3 Top 5 Top 7 Top 10
MS/MS Scan Events
ProteinsClusters
Rameh Hafezi1, Andrew Guzzetta2, Mark Friedrichs1, Rohan Thakur2 , Ashok Dongre1
1Bristol-Myers Squibb Company, Pennington, NJ 2ThermoElectron, San Jose, CA
Using the Yeast Proteome to Characterize Linear Ion Trap Performance
ASMS Poster # TPX 453
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Alprazolam Quantification 1 pg on column, 2.1mmx50, 400 uL/min, 10 uL inj
RT: 0.00 - 1.00
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0Time (min)
0
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40
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100
0
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80
100
Rel
ativ
e Ab
unda
nce
930.51
940.5292
0.51
910.50
950.52
900.50
960.53
890.49 98
0.54 1030.57
810.53
820.54
800.53
840.55 87
0.5779
0.5272
0.471030.68
1450.96
1290.85
190.12
470.31
600.39
90.06
12 scans
5 scans
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Alprazolam Quantification 1 pg on column, 2.1mmx50, 400 uL/min, 10 uL inj
RT: 0.00 - 1.00
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0Time (min)
0
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RT: 0.51AA: 253107SN: 224RMS
RT: 0.53AA: 76541SN: 50RMS
No smoothing !
100
80
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High Sensitivity High Sensitivity QuantitationQuantitation
345704335327273535483356914934551201.4651187.69350990420000
3717893829703571113759793363625.0818518.893648422000
1773712036851914971878741821805.3310046.801885211000
998699964910361198619906254.854778.6898475500
45783399174656841959457056.582894.6743986200
21684239692363121590226094.801089.6922697100
100021118911066104611464616.011837.271147350
inj #5inj #4inj #3inj #2inj #1%rsdstdevavgfg
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High Sensitivity High Sensitivity QuantitationQuantitationAlprazolam Quantification – 6 orders of linear dynamic range
0.00E+00
1.00E+09
2.00E+09
3.00E+09
4.00E+09
5.00E+09
6.00E+09
7.00E+09
8.00E+09
0.0E+00 2.0E+06 4.0E+06 6.0E+06 8.0E+06 1.0E+07 1.2E+07
R2= 1
0.00E+00
2.00E+05
4.00E+05
6.00E+05
8.00E+05
1.00E+06
0.0E+002.0E+024.0E+026.0E+028.0E+021.0E+031.2E+03
R2= 0.9997
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Two Detectors Two Detectors -- 2x Signal2x Signal
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5Time (min)
020
40
60
Rel
ativ
e Ab
unda
nce NL: 9.38E11
TIC MS
NL: 6.92E9
200 400 600 800 1000 1200 1400 1600 1800 2000
204060
100
Rel
ativ
e Ab
unda
nce 1522 1622.1422
17221322
18221222
19225241122195
First Detector Second DetectorBoth Detectors
Both Detectors
100
80
80
m/z
NL: 3.46E9
200 400 600 800 1000 1200 1400 1600 1800 2000m/z
204060
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Rel
ativ
e Ab
unda
nce 1522 16221422
172213221822
12221922524 1122195
Single Detector80
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Better CoverageBetter Coverage withwith 22 DetectorsDetectors200 200 amolsamols of Myoglobin Digest Injectedof Myoglobin Digest Injected
0.00%5.00%
10.00%15.00%20.00%25.00%
1 Det 2 Det
Number of Detectors
% C
over
age
(AA)
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3D Trap - Myoglobin
200 400 600 800 1000 1200 1400 1600 1800 2000m/z
0102030405060708090
100
Rel
ativ
e Ab
unda
nce
893.1
1327.8
1008.8808.5837.9 1134.71220.1 1417.3942.4 1020.1
1426.7
1230.1772.5 1060.3
1479.5 1762.81707.51550.9707.9 1884.11671.7
1879.8689.8
5000 6000 7000 8000 9000 10000 11000 12000 13000 14000 15000 16000 17000 18000 19000mass
0102030405060
Rel
ativ
e Ab
unda
nce
16950.0
Deconvolution
Single Scan Scan Time = .50 sec
100908070
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3D-Trap Myoglobin
200 400 600 800 1000 1200 1400 1600 1800 2000m/z
Rel
ativ
e Ab
unda
nce
848.7 998.1 1211.81061.4
1304.7
808.31413.4
1953.8
772.7 1543.9 1695.71769.8
0102030405060
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100
70
90 20 Spectra Avg.Scan Time = 10.2 sec
5000 6000 7000 8000 9000 10000 11000 12000 13000 14000 15000 16000 17000 18000 19000mass
102030405060708090
100
Rel
ativ
e Ab
unda
nce
16953.0
Deconvolution
942.6
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2D Linear Trap - Myoglobin
200 400 600 800 1000 1200 1400 1600 1800 20000102030405060
Rel
ativ
e Ab
unda
nce
942.8998.3893.3 1060.3
1211.81131.0
848.6
1304.9808.3 1413.6
771.6
738.1681.3 1695.9
652.9 1549.9
Single ScanScan Time = .18 sec
100908070
m/z
5000 6000 7000 8000 9000 10000 11000 12000 13000 14000 15000 16000 17000 18000 19000mass
0102030405060708090
100
Rel
ativ
e Ab
unda
nce
16951.0
Deconvolution
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Even Higher Resolution !!!- Scan rate of 27 amu/sec Single Scan Data!!!
195.1250 1522.0524.3
1520 1522 1524 1526m/z
1523.0
1524.01525.0
∆M =.05 amu
M/∆M = 30440
522.0 524.0 526.0 528.0m/z
525.3
526.3527.3
528.3
∆M =.05 amu
192 194 196 198
196.1
197.1
∆M = .05 amu
M/∆M = 3900 M/∆M = 10500
m/z
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MSn on Low Abundance Ions
500 1000 1500 2000m/z
0
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Rel
ativ
e Ab
unda
nce
1521.81321.9
1221.8 1621.9524.3
1121.9 1721.91107.91021.9540.3195.1 907.9 1821.6
500 1000 1500 2000
524.1
200 250 300 350 400 450 500 550 600m/z
0
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Rel
ativ
e Ab
unda
nce
288.0
271.0
453.0
489.0418.0376.0
229.0 507.0407.0352.0237.0181.0 306.0
200 250 300 350 400 450 500 550 600m/z
306.0
m/z510 515 520 525 530 535
524.1
m/z
295 300 305 310 315 320m/z
306.0
MS
MS/MS m/z 524
m/z 524 Isolation
m/z 306 Isolation
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MSn on Low Abundance Ions
100 120 140 160 180 200 220 240 260 280 300 320m/z
0
20
40
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Rel
ativ
e Ab
unda
nce
289.0
175.0263.0158.0 247.0 271.0
140.0112.0 243.1 305.9
168 170 172 174 176 178 180 182m/z
175.0
174.0
180.1175.7 177.9
MS3
524 306MS3
524 306
168 170 172 174 176 178 180 182m/z
0
20
40
60
80
100
Rel
ativ
e Ab
unda
nce
175.1
60 80 100 120 140 160 180 200m/z
0
20
40
60
80
100R
elat
ive
Abun
danc
e146.0
126.098.0 148.655.9 103.9
m/z 175 Isolation
MS4
306 175524
168 170 172 174 176 178 180 182m/z
0
20
40
60
80
100
Rel
ativ
e Ab
unda
nce
174.0
60 80 100 120 140 160 180 200m/z
0
20
40
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80
100
Rel
ativ
e Ab
unda
nce
157.9
157.160.0
70.0 115.072.0
m/z 174 Isolation
MS4
306 174524
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MSn on Low Abundance Ions
60 80 100 120 140 160 180 200m/z
0
20
40
60
80
100
Rel
ativ
e Ab
unda
nce
146.0
126.098.0 148.655.9 103.9
60 80 100 120 140 160 180 200m/z
0
20
40
60
80
100
Rel
ativ
e Ab
unda
nce
146.0
126.098.0103.9 146.855.9 180.4
MS4
306 175524
Single Scan
20 Scans Avg
60 80 100 120 140 160 180 2000
20
40
60
80
100
Rel
ativ
e Ab
unda
nce
157.9
60.0
70.0 115.072.0
158.8
60 80 100 120 140 160 180 200m/z
0
20
40
60
80
100
Rel
ativ
e Ab
unda
nce
158.0
59.9 116.0
130.169.9 111.9
83.4 119.4
m/z
306 174524MS4
20 Scans Avg
Single Scan
29
Trapping of Injected Ions
3D Trap
2D Trap
