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A PowerPoint review of photomicrographs depicting the various histological features of compact bone. By Timothy Ballard, UNC Wilmington. Licensed under a Creative Commons License: Attribution Non-Commercial-NoDerivs. From http://www.lifescitrc.org/resource.cfm?submissionID=8987.
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HISTOLOGY REVIEWHISTOLOGY REVIEWBone TissueBone Tissue
Dr. Tim BallardDr. Tim Ballard
Department of Biology and Marine BiologyDepartment of Biology and Marine Biology
Human bone – ground – cross section -- India ink – 4x objective
The arrowheads indicate osteons or Haversian systems. An osteon is that bony tissue surrounding and a part of a single Haversian canal.
Compact boneCompact bone
This is a section of dried and long dead ground compact bone. The living cells are gone, leaving behind spaces that can be back-filled with India ink.
Human bone – ground – cross section -- India ink – 10x objective
Interstitial lamellae are layers of old bone tissue from an osteon that is bring destroyed by remodeling.
Compact boneCompact bone
osteon
interstitial lamellae
Human bone – ground – cross section -- India ink – 20x objective
Each osteon runs more-or-less parallel to the long axis of the bone. If you see a canal that is perpendicular, it is a Volkmann’s canal.
Compact boneCompact bone
Haversian canal
osteocytic lacuna
lamellae
interstitial
lamellaeinterstiti
al lamellae
Human bone – ground – cross section -- India ink – 40x objective
Canaliculi are tiny canals passing through the bone matrix, connecting one osteocyte to another, ultimately connecting them all to the Haversian canal.
Compact boneCompact bone
Haversian canal
osteocytic lacuna
lamellae canalicul
i
end