High quality costs less - Biocenter.hu

savings

Save on Thermo Scientific products

•AccellsiRNAReagents

•2nmolpre-designedsiRNA

•GenomeScaleLentviralLibrary

•GeneJETPurificationKits

•Maxima&RevertAidReverseTranscriptases

•Maxima&RevertAidcDNASynthesisKits

•PhusionHigh-FidelityDNAPolymerases

•PhireHotStartIIDNAPolymerase

•Maxima&DreamTaqDNAPolymerases

•FastAPAlkalinePhosphatase&ExonucleaseI

•GeneRulerDNALadders

High quality costs less www.thermoscientific.com/onebio

A quarterly publication containing special offers for significant savings on a variety of molecular biology products.

Molecular Biology MarketplaceThermo Scientific

Individual siRNAs are now offered at a smaller quantity to provide better value for your gene silencing needs.

• Available in Thermo Scientific ON-TARGETplus and siGENOME product lines

• Genome-wide targets for human, mouse and rat• Minimum order of 4 siRNAs at the 2nmol size,

any gene, any species

Search for your gene of interest and place your order at: www.thermoscientific.com/accell

Accell siRNA reagents for difficult-to-transfect cellsThermo Scientific Accell siRNA achieves what no other product can claim: delivery into difficult-to-transfect cells without transfection reagents, virus or electroporation.

• Patented siRNA modifications facilitate uptake, stability and efficient knockdown• Proven performance in neuronal, immunological and primary cells• Appropriate for use in vivo systems

Description Cat. No. **These are agnostic product identifiers. Actual catalog numbers are gene specific

Accell SMARTpool siRNA - 5, 10, 20 or 50 nmol E-HUMAN-XX, E-MOUSE-XX, E-RAT-XX

Accell Set of 4 siRNA - 2, 5, 10 or 20 nmol EQ-HUMAN-XX, EQ-MOUSE-XX, EQ-RAT-XX

*To redeem, please add two or more qualifying Accell siRNA reagents to your order and enter the promotion code ACCELLBG. Offer does not apply to Set of 4 Upgrade. Offers cannot be combined, see website for more details. Offers are valid until December 31, 2012.

(A) Efficient silencing and low cytotoxicity of CD4+ T cells upon Accell siRNA delivery. Primary CD4+ T cells stimulated with anti CD3/CD28 beads and 30 u/mL of rIL-2 overnight. Accell siRNA delivered at 1 µM (100,000 cells/well, 2% FBS RPMI and 30 u/mL of rIL-2). Analyzed at 96 hrs. (B and C) Efficient delivery of Accell siRNA in primary rat cortical neurons. Confocal microscopy (Zeiss LSM 510) reveals virtually all neurons are positive for uptake of Accell GAPD Red Control siRNA. Scale bar 10 μm.

B C1.25

1.00

0.75

0.50

0.25

0UT NTC GAPDH UT NTC ITK UT NTC LCK

target KDcell viability

GAPDH ITK LCK

Rela

tive

targ

et e

xpre

ssio

n (N

TC=1

)Re

lativ

e ce

ll vi

abili

ty (U

T=1)

A

NEW! 2 nmol pre-designed siRNAGood things now come in smaller packages

Learn more at: www.thermoscientific.com/new-2nmol-siRNA

Buy 1, Get 1 Free*

Description Cat. No. **These are agnostic product identifiers. Actual catalog numbers are gene specific

Accell SMARTpool siRNA - 5, 10, 20 or 50 nmol E-HUMAN-XX, E-MOUSE-XX, E-RAT-XX

Accell Set of 4 siRNA - 2, 5, 10 or 20 nmol EQ-HUMAN-XX, EQ-MOUSE-XX, EQ-RAT-XX

Coming Soon! Genome Scale Lentiviral Library

The CCSB– Broad Lentiviral Expression Library A genome-scale collection of sequence-confirmed human open reading frames (ORFs) in an expression-ready lentiviral vector. Developed by researchers at the Center for Cancer Systems Biology and The Broad Institute, this library enables large-scale overexpression screening in virtually any cell type.

• Lentiviral expression vector – permits transduction of almost any mammalian cell

• CMV promoter drives strong expression of the ORF construct (hCMV)

• Blasticidin resistance allows for multiplex experiments in cells already expressing Puromycin or Neomycin resistance

• V5 tag facilitates pull-downs and labeling of expressed ORFs via immunofluorescence

The Human ORFeome v8.1 (hORFeome v8.1) A fully-sequenced, Gateway-adapted library of human open reading frames (ORFs). Developed at the Dana-Farber Cancer Institute Center for Cancer Systems Biology, the hORFeome v8.1 contains over 11,000 unique genes, and can be used to easily transfer thousands of open reading frames into your choice of expression systems.

• Fully sequenced human open reading frames• Gateway-adapted ORFs can be transferred to any

compatible expression system• Stop codons are removed to allow for C-terminal

fusion tags

Amp R F1 oripUC ori

hCMV

BsdRRRE WPRE

5' LT

R

3' SIN LTR

Ψ

pLX304-Blast-V5

PGK

attR2

pUC Origin

ORFattR1

SpectR

pDONR223

v5ORF stopv5

Amp R F1 oripUC ori

hCMV

BsdRRRE WPRE

5' LT

R

3' SIN LTR

Ψ

pLX304-Blast-V5

PGK

attR2

pUC Origin

ORFattR1

SpectR

pDONR223

v5ORF stopv5

Available formats:• Genome-scale Library• Custom Libraries• Individual Clones

Learn more at www.thermoscientific.com/HORFeomeV8.1

The molecular cloning workflow encompasses several steps: from isolation of the starting material through the creation of a recombinant plasmid DNA. Thermo Scientific products provide the best genetic content, superior enzymes and advanced PCR tools to support your cloning workflow on every step of the way.

This Marketplace offering includes some of the key products required in cloning. For more information and additional products for the cloning workflow please visit www.thermoscientific.com/cloningtools.

Accelerate your molecular cloning workflow with high quality Thermo Scientific products!

LigationElectrophoresisModification of DNA Ends

Restriction Digestion

PCR andRT-PCR

DNA & RNAPurification Kits

Pre-madecDNA & ORFs

25% OFF*

*Please quote promotion code MPD36. Offers valid until December 31, 2012. Not to be combined with any other promotions or quotes.

Thermo Scientific GeneJET Plasmid Miniprep Kit• Efficient – high yields of up to 20 µg of high quality plasmid DNA• Fast – procedure takes less than 14 minutesThermo Scientific GeneJET PCR Purification Kit• DNA recovery up to 100% (25 bp – 20 kb)• Effective removal of primers, unincorporated nucleotides, enzymes and saltsThermo Scientific GeneJET Gel Extraction Kit• DNA recovery up to 95% (25 bp – 20 kb)• Purify DNA fragments from standard or low-melt agarose gels run with TAE or TBE buffer

High yields of purified plasmid DNA and DNA fragments

A pBluescript-based high copy number plasmid was purified from E. coli culture with 6 different GeneJET™ spin columns.

M – Thermo Scientific FastRuler DNA Ladder, High Range, ready-to-use (#SM1123)1-6 – plasmid DNA isolated with 6 different GeneJET spin columns

Consistent and Reproducible Miniprep Yields

RelaxedSupercoiled

M 1 2 3 4 5 6

Cat. No. Description Size

K0502GeneJET Plasmid Miniprep Kit

50 preps

K0503 250 preps

K0701GeneJET PCR Purification Kit

50 preps

K0702 250 preps

K0691GeneJET Gel Extraction Kit

50 preps

K0692 250 preps

Discover new generation reverse transcription enzymes for enhanced performance!

Thermo Scientific reverse transcription product line offers enzymes for your every need. Whether you require highest thermal stability and increased sensitivity or just a routine reverse transcriptase, we can offer you a high quality product.

Test our new Thermo Scientific Maxima H Minus Reverse Transcriptase developed via in vitro evolution and the Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit** for additional convenience. Due to its high processivity, thermostability and eliminated RNase H activity, Maxima™ H Minus Reverse Transcriptase is capable of full length cDNA synthesis even from very long RNA transcripts making it ideal for cloning experiments. Combine Maxima H Minus RT with Thermo Scientific Phusion High-Fidelity DNA Polymerase for best results in DNA fragment generation. Use Thermo Scientific RiboLock RNase Inhibitor with our RT enzymes for reliable RNA sample protection and efficient inhibition of RNAses A, B and C.


**NOTE: RevertAid Premium First Strand cDNA Synthesis Kit is now Maxima H Minus First Strand cDNA Synthesis Kit.

25% OFF*

LigationElectrophoresisModification of DNA ends


PCR andRT-PCR


Pre-madecDNA & ORFs



PCR andRT-PCR


Pre-madecDNA & ORFs

M-MuLV RTMaxima H Minus RT

M 0.5 µg total RNA 1 µg total RNA M 0.5 µg total RNA 1 µg total RNA M

Maxima H Minus RT is capable of full-length cDNA synthesis even from 13 kb RNA targets. 13.3 kb RNA target was amplified in two-step RT-PCR. 1 µg and 500 ng of total RNA from mouse were used in a reverse transcription reaction using Maxima H Minus RT and M-MuLV RT in their optimal conditions. Synthesized cDNA was used as a template in PCR with Phusion DNA Polymerase and primers specific for mouse dystrophin gene.M – Thermo Scientific ZipRuler Express DNA Ladder 2 (#SM1373)

FormoreinformationaboutthefeaturesandcharacteristicsofthedifferentThermoScientificRTenzymes,pleaserefertothePCRandqPCRProductGuideorwww.thermoscientific.com/rt

NEW


EP0751Maxima H Minus Reverse Transcriptase

2000 U

EP0752 10000 U

EP0753 4x10000 U

K1651 Maxima H Minus First Strand cDNA Synthesis Kit**

20 rxns

K1652 100 rxns

EP0741

Maxima Reverse Transcriptase

2000 U

EP0742 10000 U

EP0743 4x10000 U

K1641 Maxima First Strand cDNA Synthesis Kit for RT-qPCR

50 rxns

K1642 200 rxns

EP0451 RevertAid™ H Minus Reverse Transcriptase

10000 U

EP0452 5x10000 U

K1631 RevertAid H Minus First Strand cDNA Synthesis Kit

20 rxns

K1632 100 rxns

EP0441RevertAid Reverse Transcriptase

10000 U

EP0442 5x10000 U

K1621 RevertAid First Strand cDNA Synthesis Kit

20 rxns

K1622 100 rxns

EO0381

RiboLock™ RNase Inhibitor, 40 U/µL

2500 U

EO0382 4x2500 U

EO0384 24x2500 U

Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit• Reliable synthesis of full-length double-stranded cDNA• Fast – procedure completed in less than 2 hours• Convenient – premixed components• Complete – includes primers, controls and residual RNA removal reagent

Efficient synthesis of double-stranded cDNA

Total Jurkat RNA was used in a ds cDNA synthesis reaction with the Maxima H Minus Double-Stranded cDNA Synthesis Kit and kits from other vendors.

Efficient synthesis of ds cDNA with the Maxima H Minus Double-Stranded cDNA Synthesis Kit

Thermo ScientificVendor AVendor B

100908070605040302010

0Thermo

Scientificvendor

Avendor

B

Yiel

d, %

NOTE: RevertAid Premium Double-Stranded cDNA Synthesis Kit is now called Maxima H Minus Double-Stranded cDNA Synthesis Kit.


Thermo Scientific Phusion High-Fidelity DNA Polymerases • A golden standard for DNA fragment generation requiring high fidelity• Unparalleled speed and yields with less enzyme• Excellent choice for 2-step RT-PCR with Maxima H Minus RTThermo Scientific Phire Hot Start II DNA Polymerase• Provides speed and allows longer amplicons for sensitive detection of your target• Minimal reaction optimization due to high inhibitor tolerance and high yieldsThermo Scientific Maxima Hot Start Taq DNA Polymerase• Specificity from hot start modification to target checking• Convenient master mix format for easier reaction set-upThermo Scientific DreamTaq DNA Polymerase• Routine target checking reactions for your cloning experiments• Longer amplicons than with Taq polymerase

PCR enzymes for DNA fragment generation and target checking!

FormoreinformationaboutthefeaturesandcharacteristicsofthedifferentThermoScientificDNApolymerases,pleaserefertothePCRandqPCRProductGuideorwww.thermoscientific.com/pcr


25% OFF*

30% OFF*

Cat. No. Description SizeF-530S

Phusion™ High-Fidelity DNA Polymerase100 U

F-530L 500 U

F-531S Phusion High-Fidelity PCR Master Mix with HF Buffer

100x50 µL rxns

F-531L 500x50 µL rxns

F-548SPhusion Flash High-Fidelity PCR Master Mix

100x20 µL rxns


F-549S Phusion Hot Start II High-Fidelity DNA Polymerase

100 U

F-549L 500 U

F-122SPhire™ Hot Start II DNA Polymerase

500x20 µL rxns


EP0602 Maxima Hot Start Taq DNA Polymerase

500 U

EP0603 5x500 U

K1052 Maxima Hot Start PCR Master Mix 500x50 µL rxns

K1062 Maxima Hot Start Green PCR Master Mix 500x50 µL rxns

EP0702 DreamTaq™ DNA Polymerase

500 U

EP0703 5x500 U

EP0712 DreamTaq Green DNA Polymerase

500 U

EP0713 5x500 U

K1072 DreamTaq PCR Master Mix 1000x50 µL rxns

K1082 DreamTaq Green PCR Master Mix 1000x50 µL rxns


K2561Maxima H Minus Double-Stranded cDNA Synthesis Kit

10 rxns

Thermo Scientific FastAP Alkaline Phosphatase and Exonuclease I• Fast – FastAP dephosphorylates all types of DNA ends in 10 min• Efficient – FastAP and ExoI remove the excess of primers and unincorporated dNTPs without any sample loss• Convenient – one tube, 30 min enzymatic procedure• Economical – 70% less expensive than spin column-based clean-up kits• Compatible – following clean-up PCR products can be used for sequencing without further purification steps

Fast dephosphorylation and PCR clean-up

Searching for the perfect ladder? – Look no further!Thermo Scientific GeneRuler DNA Ladders• Broad range and sharp bands• Bright reference bands• Easy DNA quantification• Supplied with 6X loading dye

Sequencing

Column based PCR product purification

Enzymatic PCR product clean up

PCR mixAdd FastAP & Exo I

Add Binding Buffer

Bind

Centrifuge

Wash

Elute

Centrifuge

Sequencing

PCR mixture 5 µlExonuclease I 0.5 µl (10 U)FastAP Thermosensitive Alkaline Phosphatase

1 µl (1 U)

Comparison of procedures

Incubate 15 min at 37° CIncubate 15 min at 85° C

25% OFF*

25% OFF*



PCR andRT-PCR


Pre-madecDNA & ORFs



PCR andRT-PCR


Pre-madecDNA & ORFs

GeneRuler 1 kb Plus DNA Ladder SM1331/2/3/4

0.5 µg/lane, 1X TAE, 7 V/cm

bp ng/0.5 µg

7000 2010000 20

4000 20

700 25500 75

300 25

75 25

400 25

1000 25

2000 20

3000 20

5000 75

1500 80

20000 20

200 25

1% a

garo

se

Efficient synthesis of double-stranded cDNA


EF0651 FastAP™ Thermosensitive Alkaline Phosphatase

1000 U

EF0652 5000 U

EN0581Exonuclease I (ExoI)

4000 U

EN0582 20000 U

Cat. No. Description Size Range, bpSM1331 GeneRuler 1 kb Plus DNA

Ladder500 appl. 75-20000

SM1332 2500 appl. 75-20000

SM1333 GeneRuler 1 kb Plus DNA Ladder, ready-to-use

500 appl. 75-20000

SM1334 100 appl. 75-20000

SM0311GeneRuler 1 kb DNA Ladder

500 appl. 250-10000

SM0312 2500 appl. 250-10000

SM0313 GeneRuler 1 kb DNA Ladder, ready-to-use

500 appl. 250-10000

SM0314 100 appl. 250-10000

SM0321 GeneRuler 100 bp Plus DNA Ladder

100 appl. 100-3000

SM0322 500 appl. 100-3000

SM0323 GeneRuler 100 bp Plus DNA Ladder, ready-to-use

100 appl. 100-3000

SM0324 500 appl. 100-3000

SM0241 GeneRuler 100 bpDNA Ladder

100 appl. 100-1000

SM0242 500 appl. 100-1000

SM0243 GeneRuler 100 bp DNA Ladder, ready-to-use

100 appl. 100-1000

SM0244 500 appl. 100-1000



• State-of-the-artproductsfromtheThermoScientificABgene,FinnzymesandFermentasproductlines.

• ContainsacompleteworkflowofPCRandqPCRproducts,includingreagents,instrumentsandplasticconsumables.

• Featuresproductselectiontablestoassistinfindingthebestsolutionforyourapplication.

© 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

Life Science Research

• Find information about state-of-the-art Thermo Scientific Molecular Biology products.

• Contains details for cloning workflow solutions, FastDigest restriction enzymes, GeneJET nucleic acid purification kits, GeneRuler DNA ladders and more...

• Supplied with a Free FastDigest restriction enzyme reaction conditions poster.

Single letter code B = C, G or T K = G or T R = G or A W = A or T

D = A, G or T M = A or C S = C or G Y = C or T

H = A, C or T N = G, A, T or C V = A, C or G

Cleavage blocked or impaired by Dam methylation within the recognition sequence

Cleavage blocked or impaired by Dcm methylation within the recognition sequence

Cleavage blocked or impaired by CpG methylation within the recognition sequence

Overlapping Dam methylation may block or impair DNA cleavage

Overlapping Dcm methylation may block or impair DNA cleavage

Overlapping CpG methylation may block or impair DNA cleavage

Methylation sensitivity

For detailed information, see Certificate of Analysis supplied with each product.

EnzymeSpecificity 5’→ 3’ Reaction temperature, °C

Digestion time with 1 µl of FastDigest enzyme, min

bp from end of DNA required for complete digestionThermal inactivation

Incubation time without star activity, hours

Methylation sensitivity Catalog #Package size,reactions

Lambda DNA Plasmid DNA PCR product Genomic DNA

1 µg/20 µl1 µg/20 µl ~0.2 µg/30 µl 1 µg/10 µl

FastDigest AatIIGACGT↓C

3715

2015

15

580°C, 5 min

16

FD099450

FastDigest AccI (XmiI)GT↓MKAC

375

560

5

265°C, 5 min

16

FD148450

FastDigest Acc65IG↓GTACC

375

55

5

365°C, 5 min

16

FD0904100

FastDigest AciI (SsiI)CCGC(-3/-1)↓

375

55

5

265°C, 5 min

4

FD179420

FastDigest AclI (Psp1406I)AA↓CGTT

375

55

5

365°C, 5 min

16

FD094420

FastDigest AcuI (Eco57I)*

CTGAAG(16/14)↓

3715

3015

15

265°C, 5 min

16no effect

FD034420

FastDigest AfeI (Eco47III)AGC↓GCT

375

55

5

465°C, 5 min

16

FD032420

FastDigest AflII (BspTI)C↓TTAAG

375

55

5

4

No16

no effectFD0834

150

FastDigest AgeI (BshTI)A↓CCGGT

375

55

5

380°C, 5 min

16

FD146420

FastDigest AjuI *

↓(7/12)GAA(N)7TTGG(11/6)↓37

55

105

ND65°C, 5 min

16no effect

FD195420

FastDigest AleI (OliI)CACNN↓NNGTG

375

510

5

365°C, 5 min

6

FD163420

FastDigest AluI

AG↓CT

3715

1515

15

465°C, 5 min

16no effect

FD0014100

FastDigest Alw21IGWGCW↓C

375

55

5

180°C, 20 min

16no effect

FD0024100

FastDigest Alw26IGTCTC(1/5)↓

375

55

5

165°C, 5 min

16

FD0034100

FastDigest AlwNI (CaiI)CAGNNN↓CTG

375

55

5

465°C, 10 min

16

FD139450

FastDigest ApaIGGGCC↓C

375

520

10

265°C, 5 min

16

FD1414300

FastDigest ApaLI (Alw44I)G↓TGCAC

375

6010

5

480°C, 5 min

16

FD0044200

FastDigest AscI (SgsI)GG↓CGCGCC

375

55

5

265°C, 20 min

16

FD1894100

FastDigest AseI (VspI)AT↓TAAT

375

55

30

265°C, 5 min

16no effect

FD0914200

FastDigest AsiSI (SfaAI)GCGAT↓CGC

37no sites

560

5

580°C, 5 min

6

FD2094100

FastDigest AvaI (Eco88I)C↓YCGRG

375

55

5

265°C, 5 min

16

FD0384200

FastDigest AvaII (Eco47I)G↓GWCC

375

55

5

180°C, 20 min

16

FD0314200

FastDigest AvrII (XmaJI)C↓CTAGG

375

105

10

2

No16

no effectFD1564

20

FastDigest BamHIG↓GATCC

375

55

5

280°C, 5 min

1no effect FD0054/5 800/2500

FastDigest BanI (BshNI)G↓GYRCC

375

605

15

265°C, 10 min

16

FD1004300

FastDigest BbsI (BpiI)GAAGAC(2/6)↓

375

55

5

165°C, 10 min

16no effect

FD101420

FastDigest BbvI (Lsp1109I)GCAGC(8/12)↓

375

55

10

265°C, 5 min

0.5no effect

FD2074100

FastDigest BclI

T↓GATCA

375

515

5

380°C, 20 min

16 FD0724

300

FastDigest BfaI (FspBI)C↓TAG

375

155

20

380°C, 5 min

16no effect

FD176450

FastDigest BglI

GCCNNNN↓NGGC

375

55

5

365°C, 5 min

2

FD0074200

FastDigest BglIIA↓GATCT

375

2030

20

3

No16

no effectFD0084

200

FastDigest BlpI (Bpu1102I)GC↓TNAGC

375

55

5

280°C, 5 min

16no effect

FD009450

FastDigest Bme1580I (BseSI) GKGCM↓C

375

55

30

380°C, 15 min

16no effect

FD144450

FastDigest BmtI (BspOI)GCTAG↓C

375

515

5

380°C, 5 min

1no effect

FD204420

FastDigest BplI *

↓(8/13)GAG(N)5CTC(13/8)↓37

1520

3030

ND65°C, 5 min

16no effect

FD131420

FastDigest BpmI (GsuI)CTGGAG(16/14)↓

3015

3015

15

265°C, 5 min

16

FD046420

FastDigest Bpu10ICCTNAGC(-5/-2)↓

3715

1530

15

380°C, 5 min

1no effect

FD118420

FastDigest BsaAI (Ppu21I)YAC↓GTR

375

55

5

165°C, 5 min

6

FD197450

FastDigest BsaBI (BseJI)GATNN↓NNATC

655

55

5

3

No1

FD1714200

FastDigest BsaHI (Hin1I)GR↓CGYC

375

55

5

165°C, 5 min

16

FD0474100

FastDigest BsaJI (BseDI)C↓CNNGG

375

55

5

380°C, 5 min

16no effect

FD108420

FastDigest BseGIGGATG(2/0)↓

375

55

5

280°C, 5 min

16no effect

FD0874100

FastDigest BseNIACTGG(1/-1)↓

655

55

5

280°C, 5 min

16no effect

FD0884100

FastDigest BseXIGCAGC(8/12)↓

6515

1515

15

380°C, 15 min

16no effect

FD145420

FastDigest Bsh1236ICG↓CG

375

55

5

180°C, 10 min

16

FD0924100

FastDigest BsiEI (Bsh1285I)CGRY↓CG

3715

1515

15

380°C, 15 min

1 FD0894

100

FastDigest BsiWI (Pfl23II)C↓GTACG

3715

1515

15

365°C, 5 min

16

FD085450

FastDigest BslI (BseLI)CCNNNNN↓NNGG

375

55

5

2

No16

FD1204

100

FastDigest BsmBI (Esp3I)*

CGTCTC(1/5)↓

375

55

5

165°C, 10 min

6

FD045420

FastDigest BsmFI (FaqI)*GGGAC(10/14)↓

3715

1515

15

365°C, 5 min

16

FD181420

FastDigest Bsp119ITT↓CGAA

375

55

5

180°C, 5 min

16

FD0124200

FastDigest Bsp120IG↓GGCCC

375

515

5

380°C, 10 min

16

FD0134200

FastDigest Bsp1286I (SduI)GDGCH↓C

375

55

5

280°C, 15 min

1no effect

FD065450

FastDigest Bsp1407IT↓GTACA

375

55

5

380°C, 10 min

16no effect

FD0934100

FastDigest BspCNI (BseMII) *CTCAG(10/8)↓

5515

3015

15

280°C, 5 min

16no effect

FD140420

FastDigest BspHI (PagI)T↓CATGA

375

105

10

380°C, 5 min

0.5 FD1284

50

FastDigest BspMI (BveI)*

ACCTGC(4/8)↓

3715

1560

20

565°C, 5 min

16

FD174450

FastDigest BsrBI (MbiI)CCGCTC(-3/-3)↓

375

55

5

365°C, 5 min

16

FD1274100

FastDigest BsrDI (BseMI)GCAATG(2/0)↓

5515

1515

15

380°C, 5 min

16no effect

FD126420

FastDigest BsrFI (Cfr10I)R↓CCGGY

375

510

5

2

No2

FD0184

50

FastDigest BssHII (PteI)G↓CGCGC

375

55

5

380°C, 5 min

16

FD213420

FastDigest BstXICCANNNNN↓NTGG

375

55

5

480°C, 5 min

0.5

FD1024100

FastDigest BstZ17I (Bst1107I) GTA↓TAC

375

55

10

3

No6

FD0704

100

FastDigest Bsu36I (Eco81I)CC↓TNAGG

375

55

5

280°C, 10 min

16no effect

FD037450

FastDigest ClaI (Bsu15I)AT↓CGAT

375

55

5

265°C, 15 min

16 FD0144

100

FastDigest Csp6IG↓TAC

375

55

5

280°C, 10 min

16no effect

FD0214100

FastDigest DdeI (HpyF3I)C↓TNAG

375

55

10

365°C, 5 min

16no effect

FD188450

FastDigest DpnIGm6A↓TC

37ND

5not applicable

10

180°C, 5 min

16 does not cut dam– DNA FD1704100

FastDigest DraITTT↓AAA

375

55

5

465°C, 5 min

16no effect

FD0224200

FastDigest DraIII (AdeI)CACNNN↓GTG

375

55

5

280°C, 5 min

1

FD123450

FastDigest DrdI (AasI)GACNNNN↓NNGTC

375

55

5

180°C, 10 min

1

FD172425

FastDigest EagI (Eco52I)C↓GGCCG

375

2020

5

365°C, 5 min

16

FD033450

FastDigest Eam1105IGACNNN↓NNGTC

375

55

5

365°C, 5 min

1no effect

FD0244100

FastDigest EarI (Eam1104I)CTCTTC(1/4)↓

375

55

5

380°C, 5 min

6no effect

FD023450

FastDigest Ecl136IIGAG↓CTC

375

55

5

165°C, 5 min

6

FD0254100

FastDigest Eco31IGGTCTC(1/5)↓

375

55

5

365°C, 5 min

16

FD0294100

FastDigest Eco91IG↓GTNACC

375

55

5

265°C, 10 min

2no effect

FD0394200

FastDigest EcoNI (XagI)CCTNN↓NNNAGG

375

55

20

265°C, 5 min

16no effect

FD1304100

FastDigest EcoO109IRG↓GNCCY

375

1015

5

265°C, 5 min

16

FD0264200

FastDigest EcoRIG↓AATTC

375

520

5

280°C, 5min

0.5

FD0274/5 800/2500

FastDigest EcoRV (Eco32I)GAT↓ATC

375

55

5

2

No16

no effectFD0304

400

FastDigest EheIGGC↓GCC

375

55

5

265°C, 5 min

6

FD044450

FastDigest Fnu4HI (SatI)GC↓NGC

375

510

5

365°C, 5 min

16

FD164420

FastDigest FokI

GGATG(9/13)↓

375

55

5

165°C, 5 min

1no effect

FD2144100

FastDigest FspI (NsbI)TGC↓GCA

375

55

5

365°C, 15 min

16

FD122450

FastDigest FspAIRTGC↓GCAY

375

55

5

465°C, 5 min

16

FD166420

FastDigest HaeII (BfoI)RGCGC↓Y

375

55

5

265°C, 10 min

1

FD2184200

FastDigest HaeIII (BsuRI)GG↓CC

375

55

5

3

No16

no effectFD0154

400

FastDigest HgaI (CseI)GACGC(5/10)↓

3715

1530

15

280°C, 5 min

16

FD190420

FastDigest HhaIGCG↓C

375

55

5

1

No16

FD1854

200

FastDigest HincIIGTY↓RAC

375

55

10

165°C, 5 min

16

FD0494100

FastDigest HindIIIA↓AGCTT

375

520

10

380°C, 10 min

16no effect FD0504/5 800/2500

FastDigest HinfIG↓ANTC

375

55

5

265°C, 20 min

16

FD0804400

FastDigest HinP1I (Hin6I)G↓CGC

375

105

5

280°C, 10 min

16

FD0484200

FastDigest HpaI (KspAI)GTT↓AAC

375

55

5

365°C, 20 min

0.5

FD103450

FastDigest HpaIIC↓CGG

375

55

5

365°C, 5 min

16

FD0514200

FastDigest Hpy8IGTN↓NAC

375

55

10

280°C, 5 min

16

FD157420

FastDigest HpyF10VIGCNNNNN↓NNGC

375

55

5

280°C, 5 min

16

FD173420

FastDigest KpnIGGTAC↓C

375

55

5

380°C, 5 min

16no effect

FD0524300

FastDigest Kpn2IT↓CCGGA

375

55

5

280°C, 5 min

16

FD0534100

FastDigest MauBICG↓CGCGCG

37no sites

510

5

565°C, 5 min

16

FD208420

FastDigest MboI↓GATC

375

55

10

165°C, 15 min

16 FD0814

50

FastDigest MboIIGAAGA(8/7)↓

375

55

5

265°C, 5 min

16 FD0824

50

FastDigest MfeI (MunI)C↓AATTG

375

55

5

3

No16

no effectFD0754

50

FastDigest MluIA↓CGCGT

375

55

15

380°C, 5 min

16

FD0564100

FastDigest MlyI (SchI)GAGTC(5/5)↓

375

530

5

280°C, 5 min

1no effect

FD1374100

FastDigest MnlICCTC(7/6)↓

375

55

5

265°C, 5 min

16no effect

FD107450

FastDigest MreICG↓CCGGCG

37no sites

55

5

380°C, 5 min

16

FD202420

FastDigest MscI (MlsI)TGG↓CCA

375

530

20

380°C, 20 min

16

FD121450

FastDigest MseI (SaqAI)T↓TAA

375

55

5

465°C, 5 min

16no effect

FD217450

FastDigest MslI (RseI)CAYNN↓NNRTG

375

55

5

465°C, 20 min

16no effect

FD200420

FastDigest MspIC↓CGG

375

55

5

3

No16

no effectFD0544

400

FastDigest MssIGTTT↓AAAC

375

55

5

265°C, 10 min

16no effect

FD1344100

FastDigest MvaICC↓WGG

375

55

10

4

No1

no effectFD0554

200

FastDigest Mva1269IGAATGC(1/-1)↓

375

55

5

365°C, 5 min

16no effect

FD096450

FastDigest NaeI (PdiI)GCC↓GGC

37ND

55

5

365°C, 15 min

16

FD152450

FastDigest NciI (BcnI)CC↓SGG

375

55

5

380°C, 20 min

16

FD0064200

FastDigest NcoIC↓CATGG

375

1010

5

365°C, 15 min

16no effect FD0574/5 100/300

FastDigest NdeICA↓TATG

375

560

30

365°C, 5 min

6no effect FD0584/5 300/1000

FastDigest NheIG↓CTAGC

375

155

5

565°C, 5 min

6

FD0974100

FastDigest NlaIII (Hin1II)CATG↓

375

105

10

480°C, 5 min

16no effect

FD1834100

FastDigest NlaIV (BspLI)GGN↓NCC

375

55

5

265°C, 20 min

16

FD115420

FastDigest NmuCI↓GTSAC

375

55

10

265°C, 5 min

16

FD151420

FastDigest NotIGC↓GGCCGC

37no sites

305

10

280°C, 5 min

16

FD0594/5 50/150

FastDigest NruI (RruI)TCG↓CGA

375

55

5

5

No4

FD2154

100

FastDigest NsiI (Mph1103I)ATGCA↓T

375

55

10

365°C, 15 min

6no effect

FD0734100

FastDigest NspI (XceI)RCATG↓Y

375

55

5

265°C, 5 min

16no effect

FD147450

FastDigest PacITTAAT↓TAA

37no sites

55

5

265°C, 10 min

16no effect

FD220425

FastDigest PdmIGAANN↓NNTTC

375

55

5

265°C, 5 min

16

FD1534100

FastDigest PflMI (Van91I)CCANNNN↓NTGG

375

55

5

365°C, 10 min

6

FD0714100

FastDigest PfoI

T↓CCNGGA

375

55

5

365°C, 5 min

16 FD1754

20

FastDigest PmlI (Eco72I)CAC↓GTG

375

55

5

280°C, 10 min

1

FD0364200

FastDigest PpuMI (Psp5II)RG↓GWCCY

375

55

20

280°C, 5 min

1

FD076450

FastDigest PshAI (BoxI)GACNN↓NNGTC

375

55

5

380°C, 20 min

16

FD1434100

FastDigest PsiI (AanI)TTA↓TAA

375

55

5

565°C, 5 min

16no effect

FD206420

FastDigest PspFICCCAGC(-1/-5)↓

375

55

5

480°C, 5 min

16

FD222420

FastDigest PstI

CTGCA↓G

375

530

5

3

No16

no effect FD0614/5 800/2500

FastDigest PsuIR↓GATCY

375

55

5

280°C, 5 min

16no effect

FD1554150

FastDigest PsyIGACN↓NNGTC

375

55

5

280°C, 5 min

4no effect

FD1334100

FastDigest PvuICGAT↓CG

375

155

5

480°C, 5 min

16

FD062420

FastDigest PvuIICAG↓CTG

375

55

5

1

No0.5

no effectFD0634

400

FastDigest RsaIGT↓AC

375

55

5

3

No16

FD1124

100

FastDigest RsrII (CpoI)CG↓GWCCG

375

55

5

165°C, 5 min

16

FD074420

FastDigest SacIGAGCT↓C

375

1530

10

165°C, 5 min

16no effect

FD1134200

FastDigest SalI

G↓TCGAC

375

560

5

365°C, 10 min

16

FD0644200

FastDigest SanDI (KflI)GG↓GWCCC

375

55

10

5

No16

FD216420

FastDigest SapI (LguI)GCTCTTC(1/4)↓

375

55

5

265°C, 5 min

16no effect

FD193420

FastDigest Sau3AI (Bsp143I) ↓GATC

375

510

5

465°C, 20 min

16

FD078440

FastDigest Sau96I (Cfr13I)G↓GNCC

375

55

5

2

No16

FD0194100

FastDigest SbfI (SdaI)CCTGCA↓GG

375

55

5

3

No1

no effectFD1194

20

FastDigest ScaIAGT↓ACT

375

55

5

465°C, 10 min

16no effect

FD0434100

FastDigest ScrFI (Bme1390I) CC↓NGG

375

55

5

4

No16

FD1424

100

FastDigest SexAI (CsiI)A↓CCWGGT

375

105

15

465°C, 5 min

16no effect

FD211450

FastDigest SfaNI (BmsI)GCATC(5/9)↓

375

55

5

165°C, 5 min

16

FD212420

FastDigest SfcI (BfmI)C↓TRYAG

375

55

5

265°C, 20 min

16no effect

FD116420

FastDigest SfiI

GGCCNNNN↓NGGCC

50no sites

1515

15

5

No16

FD1824

150

FastDigest SmaICCC↓GGG

375

55

5

165°C, 5 min

16

FD0664200

FastDigest SnaBI (Eco105I)TAC↓GTA

375

55

5

365°C, 5 min

16

FD040450

FastDigest SpeI (BcuI)A↓CTAGT

37no sites

55

5

1

No16

no effectFD1254

50

FastDigest SphI (PaeI)GCATG↓C

375

55

5

565°C, 5 min

16no effect

FD060420

FastDigest SspIAAT↓ATT

375

55

30

265°C, 5 min

1no effect

FD0774100

FastDigest StuI (Eco147I)AGG↓CCT

375

55

5

380°C, 10 min

16

FD0424100

FastDigest StyI (Eco130I)C↓CWWGG

375

520

20

365°C, 5 min

16no effect

FD0414200

FastDigest SwaI (SmiI)ATTT↓AAAT

37no sites

2030

30

265°C, 15 min

1no effect

FD1244200

FastDigest TaaIACN↓GT

655

55

5

3

No16

FD1364

20

FastDigest TaiI

ACGT↓

655

55

5

2

No6

FD1144

50

FastDigest TaqIT↓CGA

655

55

5

4

No16

FD0674400

FastDigest TatI

W↓GTACW

6515

1515

20

5

No1

no effectFD1294

20

FastDigest TauIGCSG↓C

5515

6015

30

5

No16

FD1654

20

FastDigest Tfil (PfeI)G↓AWTC

375

510

5

265°C, 5 min

16

FD178450

FastDigest Tru1IT↓TAA

655

55

10

3

No2

no effectFD0984

50

FastDigest Tsp509I (TasI)↓AATT

655

55

5

3

No6

no effectFD1354

100

FastDigest TspRI (TscAI)CASTG(2/-7)↓

655

55

5

2

No6

no effectFD2104

100

FastDigest XapIR↓AATTY

375

55

10

480°C, 5 min

6no effect

FD1384100

FastDigest XbaIT↓CTAGA

375

55

10

265°C, 20 min

16 FD0684/5 300/750

FastDigest XhoIC↓TCGAG

375

55

10

280°C, 5 min

16

FD0694/5 400/1200

Activity of DNA/RNA modifying enzymes in

FastDigest Green Buffer and FastDigest Buffer

DNA/RNA modifying enzymeActivity in FastDigest Green Buffer/ FastDigest Buffer

DNA Polymerase I (#EP0041)

100%

Klenow Fragment (#EP0051)

100%

Klenow Fragment, exo – (#EP0421)

100%

T4 DNA Polymerase (#EP0061)

100%

T7 DNA Polymerase (#EP0081)

100%

T4 DNA Ligase* (#EL0011)

75-100%

Thermo Scientific FastAP Alkaline

Phosphatase (#EF0651)

100%

T4 Polynucleotide Kinase (#EK0031)

100%

*0.5 mM ATP is required for T4 DNA Ligase activity.

Standard reaction set-upComponent

VolumePlasmid DNA PCR product

Genomic DNA

unpurified purified

Nuclease-free Water15 µl

17 µl up to 30 µl 30 µl

10X FastDigest Green Buffer or 10X FastDigest Buffer 2 µl

2 µl3 µl

5 µl

DNA

2 µl (up to 1 µg)10 µl (~0.2 µg) ~0.2 μg 10 µl

(5 µg)

FastDigest enzyme1 µl

1 µl1 µl

5 µl

TOTAL volume20 µl

30 µl30 µl

50 µl

The volume of DNA can be scaled up to 10 µl or down to 0.5 µl depending on the DNA concentration.

The volume of water should be corrected to keep the indicated total reaction volume.

DNA

1 µg 2 µg 3 µg 4 µg 5 µg

FastDigest Enzyme1 µl 2 µl 3 µl 4 µl 5 µl

10X FastDigest Green Buffer or 10X FastDigest

Buffer2 µl 2 µl 3 µl 4 µl 5 µl

TOTAL volume

20 µl 20 µl 30 µl 40 µl 50 µl

Scaling up DNA digestion reaction

• 100% activity of all Thermo Scientific

FastDigest Enzymes in the universal buffer

• 100% buffer compatibility with

downstream applications• Direct loading on gel

• No star activity • Complete digestion in 5 to 15 minutes

www.thermoscientificbio.com/fastdigest

*For digestion with FastDigest BsmBI (Esp3I) add 1 mM DTT. For digestion with FastDigest AcuI (Eco57I), FastDigest AjuI and FastDigest BspCNI (BseMII) add 0.01 mM SAM. For digestion with FastDigest BpII and Fastdigest BsmFI (FaqI) add 0.05 mM SAM.

For digestion with FastDigest BspMI (BveI) add 0.5 µM oligonucleotide. No - thermal inactivation is not possible, spin column purification or phenol/chloroform extration is recommended. ND - not determined.

FastDigest™ Green Reaction Buffer

3-5 kb

10 bp

Five minute triple digestion in the

FastDigest Green Reaction Buffer

M 1 2 3 4 M

1. plasmid - undigested control

2. plasmid - digested with FastDigest EcoRI

3. plasmid - double digested with FastDigest EcoRI and FastDigest KpnI

4. plasmid - triple digested with FastDigest EcoRI, FastDigest KpnI

and FastDigest SmaIM. marker - Thermo Scientific GeneRuler Express Ladder #SM1553

Molecular BiologyTel: 1 800 340 9026Fax: 1 800 472 8322 [email protected]

www.thermoscientific.com/onebio

© 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Literature # M0018412C01U

GeneRuler 1kb Plus DNA Ladder,

#SM1331/2/3/4O’GeneRuler 1kb Plus DNA Ladder, #SM1343

GeneRuler 1kb DNA Ladder,#SM0311/2/3/4O’GeneRuler 1kb DNA Ladder,

#SM1163

1% A

garo

se

0.5µg/lane, 8cm gel1X TAE, 7V/cm, 45min

8000 30.0

10000 30.0

5000 30.0

2000 25.0

1000 60.0

500 25.0250 25.0

750 25.0

2500 25.0

3500 30.0

4000 30.0

6000 70.0

1500 25.0

3000 70.0

bp ng/0.5µg

0.5µg/lane, 8cm gel1X TAE, 7V/cm, 45min

bp ng/0.5µg

7000 20.0

10000 20.0

4000 20.0

700 25.0500 75.0300 25.0

75 25.0

400 25.0

1000 25.0

2000 20.0

3000 20.0

5000 75.0

1500 80.0

20000 20.0

200 25.0

1% A

garo

se

1.7%

Aga

rose

0.5µg/lane, 8cm gel1X TBE, 5V/cm, 1h

bp ng/0.5µg

2000 28.01500 28.01200 28.0

3000 28.0

900 27.0

500 80.0400 30.0

200 30.0100 30.0

300 30.0

600 27.0

700 27.0

800 27.0

1000 80.0

GeneRuler 100bp Plus DNA Ladder, #SM0321/2/3/4

O’GeneRuler 100bp Plus DNA Ladder, #SM1153

50 80

4020

80 40

10 124 62

20 µl/lane, 1X TBE, 7 V/cm

bp ng/20 µl ng/10 µl200 80 40

100 80 40

FastRuler Ultra Low Range DNA Ladder,

ready-to-use #SM1233

400 80 40

200 80 4050

80 40

20 µl/lane, 1X TBE, 7 V/cm

FastRuler Low Range DNA Ladder,

ready-to-use #SM1103bp ng/20 µl ng/10 µl

1500 80 40

850 80 40

FastRuler Middle Range DNA Ladder,

ready-to-use #SM1113

20 µl/lane, 1X TAE, 7 V/cm

bp ng/20 µl ng/10 µl 5000 80

40850 80

40

2000 80 40

400 80 40100 80

40

FastRuler High Range DNA Ladder,

ready-to-use #SM1123bp ng/20 µl ng/10 µl10000 80

402000 80 40

4000 80 401000 80

40500 80

4020 µl/lane, 1X TAE, 7 V/cm

4% A

garo

se

1% A

garo

se

1% A

garo

se

2% A

garo

se

1 buffer • 100% digestion • all DNA templates

Thermo Scientific FastDigest Restriction Enzymes

one place for all your molecular biology needs

A complete set of Thermo Scientific product guides NoW available!

Thermo Scientific PCR and qPCR product guide

Thermo Scientific Molecular Biology Tools product guide

Thermo Scientific RNAi and Gene Expression product guideRNAi & gene expression product guide

Thermo Scientific Molecular Biology Solutions

2012 - 2013• Findindustry-leadingsiRNA,shRNA,microRNA,cDNAand

ORFreagentsaswellasacompletesuiteofproductsfordeliveryandcontrols.

Order 1, 2 or all 3 online from www.thermoscientific.com/molbio-guides

NEW

[email protected]@thermofisher.comwww.thermoscientific.com/onebio

Literature # FMW17

DNS tisztító kitekKód Megnevezés Kiszerelés Nettó ár Akciós árK0502 GeneJET™ Plasmid Miniprep Kit 50 preps 15 400 11 150K0503 GeneJET™ Plasmid Miniprep Kit 250 preps 62 300 46 725K0691 GeneJET™ Gel Extraction Kit 50 preps 19 500 14 625K0692 GeneJET™ Gel Extraction Kit 250 preps 81 300 60 975K0701 GeneJET™ PCR Purification Kit 50 preps 19 500 14 625K0702 GeneJET™ PCR Purification Kit 250 preps 81 300 60 975

Reverz transzkriptázok, cDNS szintézis kitek, RNáz inhibítorEP0751 Maxima H Minus Reverse Transcriptase 2000 units 14 600 10 950EP0752 Maxima H Minus Reverse Transcriptase 10000 units 47 000 35 250EP0753 Maxima H Minus Reverse Transcriptase 4x10000 units 149 200 111 900K1651 Maxima H Minus First Strand cDNA synthesis kit 20 react 31 200 23 400K1652 Maxima H Minus First Strand cDNA synthesis kit 100 react 124 700 93 525EP0741 Maxima® Reverse Transcriptase 2000 units 14 600 10 950EP0742 Maxima® Reverse Transcriptase 10000 units 47 000 35 250EP0743 Maxima® Reverse Transcriptase 4x10000 units 149 200 111 900K1641 Maxima® First Strand cDNA Synthesis Kit 50 react 80 300 60 225K1642 Maxima® First Strand cDNA Synthesis Kit 200 react 241 000 180 750EP0451 RevertAid™ H Minus Reverse Transcriptase 10000 units 37 600 28 200EP0452 RevertAid™ H Minus Reverse Transcriptase 5x10000 units 150 500 112 875K1631 RevertAid™ H Minus First Strand cDNA Synthesis

Kit20 react 25 100 18 825

K1632 RevertAid™ H Minus First Strand cDNA Synthesis Kit

100 react 100 200 75 150

EP0441 RevertAid™ Reverse Transcriptase 10000 units 16 500 12 375EP0442 RevertAid™ Reverse Transcriptase 5x10000 units 66 200 49 650K1621 RevertAid™ First Strand cDNA Synthesis Kit 20 react 17 000 12 750K1622 RevertAid™ First Strand cDNA Synthesis Kit 100 react 68 100 51 075EO0381 RiboLock™ RNase Inhibitor 2500 units 15 400 11 550EO0382 RiboLock™ RNase Inhibitor 4x2500 units 54 900 41 175EO0384 RiboLock™ RNase Inhibitor 24x2500 units 242 900 182 175

Kettős szálú cDNS szintézis kitK2561 Maxima H Minus Double-strand cDNA synthesis kit 10 react 126 000 94 500

Polimerázok PCR-hezF-530L Phusion High-Fidelity DNA Polymerase 500U 128 200 89 740F-530S Phusion High-Fidelity DNA Polymerase 100U 29 000 20 300F-531L Phusion High-Fidelity PCR Master Mix with HF Buffer 500 x 50ul 196 600 137 620F-531S Phusion High-Fidelity PCR Master Mix with HF Buffer 100 x 50ul 49 100 34 370F-548L Phusion Flash High-Fidelity PCR Master Mix 500 Rxns 92 000 64 400F-548S Phusion Flash High-Fidelity PCR Master Mix 100 Rxns 23 000 16 100F-549L Phusion Hot Start II High-Fidelity DNA Polymerase 500U 157 200 110 040F-549S Phusion Hot Start II High-Fidelity DNA Polymerase 100U 34 300 24 010F-122L Phire Hot Start II DNA Polymerase 1000 x 50ul 127 300 89 110F-122S Phire Hot Start II DNA Polymerase 200 x 50ul 27 700 19 390EP0602 Maxima® Hot Start Taq DNA Polymerase 500 units 37 400 26 180EP0603 Maxima® Hot Start Taq DNA Polymerase 5x500 units 149 700 104 790K1052 Maxima® Hot Start PCR Master Mix (2X) 500 react 93 600 65 520K1062 Maxima® Hot Start Green PCR Master Mix (2X) 500 react 93 600 65 520EP0702 DreamTaq™ DNA Polymerase 500 units 19 800 13 860EP0703 DreamTaq™ DNA Polymerase 5x500 units 79 400 55 580EP0712 DreamTaq™ Green DNA Polymerase 500 units 19 800 13 860EP0713 DreamTaq™ Green DNA Polymerase 5x500 units 79 400 55 580K1072 DreamTaq™ PCR Master Mix (2X) 1000 react 105 800 74 060

Gyors defoszforilálás és enzimatikus PCR-termék tisztítás szekvenáláshozEF0651 FastAP™ Thermosensitive Alkaline Phosphatase 1000 units 17 300 12 975EF0652 FastAP™ Thermosensitive Alkaline Phosphatase 5x1000 units 69 300 51 975EN0581 Exonuclease I 4000 units 20 800 15 600EN0582 Exonuclease I 20000 units 83 400 62 550

DNS létrákSM1331 GeneRuler™ 1 kb Plus DNA Ladder 5x50 µg 29 000 21 750SM1332 GeneRuler™ 1 kb Plus DNA Ladder 25x50 µg 115 900 86 925SM1333 GeneRuler™ 1 kb Plus DNA Ladder, ready-to-use 5x50 µg 36 200 27 150SM1334 GeneRuler™ 1 kb Plus DNA Ladder, ready-to-use 50 µg 14 500 10 875SM0311 GeneRuler™ 1 kb DNA Ladder 5x50 µg 22 900 17 175SM0312 GeneRuler™ 1 kb DNA Ladder 25x50 µg 91 500 68 625SM0313 GeneRuler™ 1 kb DNA Ladder, ready-to-use 5x50 µg 26 800 20 100SM0314 GeneRuler™ 1 kb DNA Ladder, ready-to-use 50 µg 14 500 10 875SM0321 GeneRuler™ 100 bp Plus DNA Ladder 50 µg 13 800 10 350SM0322 GeneRuler™ 100 bp Plus DNA Ladder 5x50 µg 55 300 41 475SM0323 GeneRuler™ 100 bp Plus DNA Ladder, ready-to-use 50 µg 17 200 12 900SM0324 GeneRuler™ 100 bp Plus DNA Ladder, ready-to-use 5x50 µg 68 800 51 600SM0241 GeneRuler™ 100 bp DNA Ladder 50 µg 13 800 10 350SM0242 GeneRuler™ 100 bp DNA Ladder 5x50 µg 55 300 41 475SM0243 GeneRuler™ 100 bp DNA Ladder, ready-to-use 50 µg 17 200 12 900SM0244 GeneRuler™ 100 bp DNA Ladder, ready-to-use 5x50 µg 68 800 51 600

Thermo Scientific akciós árlista

Érvényes: 2012. december 17-ig

Documents

High quality costs less - Biocenter.hu