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Genomes to Life Facilities for 21st Century Biology
Facility IV Analysis and Modeling of Cellular Systems
Measure & predict dynamic events within cells.
Spatial & temporal variations in cell “state” – individual microbes to multi-cellular systems.
Facility I Production and Characterization of Proteins
Facility II Whole Proteome
Analysis
Facility IV Modeling and
Analysis of Cellular Systems
Facility III Characterization and Imaging of Molecular Machines
Molecular Machine Dynamics Reveal Function
dynamic changes in cellular localization of the DivKprotein reveal insights into the cell cycle of
Caulobacter crescentus
Capabilities Required for Measuring Individual Cells
Single microbe populations exhibit variation
Can arise from noise in gene expression
Microbial communities will be more complicated
Elowitz et al. 16 AUGUST 2002
Genomesequence
High-ThroughputCultivation
Data AnalysisData Interpretation
Data ArchivalModeling
Facility I
Facility III
Facility II
•New tools & infrastructure for unprecedented analyses of cell state
•Cell & multi-cell models
•Systems level understanding of microbes & communities
Proteomics,Regulatory
networks
Reagents,probes
Machinesfor dynamic
analysisCultures,communities
Cultures
Facility IV: Final Component of an Integrated Network for Systems Biology
Spatial, TemporalAnalyses (imaging)
Small MoleculeAnalyses
Controlled Experimental
Systems
Cultures,genes
Single Cell Expression
Spatially Resolved NMR Microspectroscopy
Spatially localized
spectroscopy
3-D µ-MRI of water distribution
in a 600-µm- diameter tumor
spheroidCombined
optical/ MR microscope
Environmentally regulated sample chamber
In situ analysis of environment, metabolites in living cells
Facility IV: Analysis and Modeling of Cellular Systems
Enable the final ascent to a systems level understanding of microbes, communities & multi-cellular organisms
Next generation biology facility to measure dynamic state of living cells – machines, metabolites, signaling…
Couple experiment, analysis, and theory in a recursive fashion to reveal networks, function