Garlic Armageddon

BioSat

Lauren Persons, Ryder Whitmire, Lenny Komow, Kirstyn Johnson,

Jon Pfohl, Dave Borncamp

October 6th 2009

Mission Overview

Mission Statement- Analyze effect of radiation and atmosphere on growth rate of bacteria

Mission Objectives- Send bacteria up with Geiger counter, pressure sensor, temperature sensor,

heater- Compare bacteria to control ground group (quantitatively and

spectrophotometrically)

Mission Expectations- Bacterial recovery rates = low compared to control

- - Radiation, (heat can be a factor)

Implications- Accidentally carrying bacteria into space

Requirements Flow Down• 2.1 Level 0 Requirements:

1. Weigh less than 850 grams, budget of $100- contains Geiger counter, Petri dish and tube (with E. coli bacteria)2. Measure amounts of radiation in near space, the effects it has on the bacteria- fulfill mission objective of documenting survival rate of bacteria exposed to radiation

• 2.2 Level 1 Requirements:1. Geiger counter = counts of radiation as function of altitude2. Petri dish and tube of e. coli placed in front of glass window and exposed to radiation from atmosphere3. Temperature sensor measures how much impact temperature has on the e. coli

Design• Two plates of bacteria:

1) will be a control

2) will be exposed to radiation of upper atmosphere

- The plates test quantifiable data

• Tubes of bacteria (in broth)

- Test spectrophotometrically

• How it will be recorded

- Radiation recorded by Geiger counter every 5 seconds (pressure recorded too)

- Will be used to plot radiation as a function of altitude

Parts List

Satellite Parts

Geiger counter Petri dish

Agar Nutrient broth

Tube + cap Glass filter

Bacteria Batteries

Dry Ice Polyolefin film

Insulation HOBO Datalogger

AVR Board Camera

Heater

Diagram

Functional Block Diagram

Switch 3Power

(Batteries)

Camera

AVR Controller

Heater

Petri Temp Sensor 1

Geiger Counter

Switch HOBO datalogger

Internal Temperature

External Temperature

Humidity

Power

Power

Power Power

Weight Budget

Component Weight (g)

Geiger Counter 102

FoamCore 75

Petri Dish 25

Pressure tube 15.2

Pressure tube teflon cap ORPressure tube nylon cap*

13.23.9

Geiger counter battery 40

Structure 25

Camera filter 30

Total 325.4

CostsMaterial/Device Cost Source

Batteries $20 Grocery Store

Geiger Counter Donated Images Co.

Polyolefin film Donated Europack Inc.

Petri Dishes Donated Biology Dept at CU

Bacteria Donated Carolina Biological

Temperature Sensor $5 Electronics Center, ITLL

Agar Donated MCDB

Dry Ice $20 Grocery Store

Pressure tube Donated Ace Glass Inc.

Insulation $10 Home Depot

Camera filter Donated B&H Photo Video

Total 65

ScheduleDate Tasks9/22/09 Conceptual Design Review9/29/09 Prototyping design complete

Authority to Proceed and Hardware Ordering

10/1/09 Design completeOrder or acquire all hardwareCompletion of Vacuum Test

10/6/09 Design Document Revisions A/BCritical Design Review

10/15/09 Testing final structure CompleteCold test

10/22/09 Completion of Geiger counter/ Radiation Test

Completion of Board and Sensors Test

10/27/09 Software Test10/29/09 In Class Mission Simulation Test10/31/09 Grow cultures both as control and

for flight11/3/09 Design Document Revision C

Launch Readiness Review11/5/09 Pre-launch Testing of Bacteria

Completed11/6/09 BalloonSat Weigh-in and Turn in11/7/09 Launch Day11/8/09 Post-launch Testing of Bacteria

Completed12/1/09 Design Document Revision D

Final Presentation12/5/09 Design Expo12/8/09 BalloonSat Hardware Turn In

Proposal Requirements

• The Geiger counter does not have an output that exceeds five volts

• The heater and insulation will keep the internal temperature above -10 degrees C

• The combined components will not exceed 850 grams (if it does exceed, modifications will need to be made)

• The team will maintain a safe environment, and take precautions to protect the electrical components

Testing• Structural Tests: Drop Test, Whip Test, Cold Test– Completed by 10/15/09

• Scientific Tests: Pressure and Temperature Testing– Bacterial Response– Geiger Counter– Pressure Tubes– Completed by 10/22/09

• Software Testing– Geiger Counter– Camera– Completed by 10/22/09

Expected Results

• Cell death by radiation– Decreased colony growth• Replica Plating

– Slower propagation in nutrient broth• Spectrophotometric Analysis

Organizational ChartProject Management:

Kirstyn Johnson, lead

Ryder Whitmire, assistant

Systems Management:

Ryder Whitmire, lead

Jonathan Pfohl, assistant

Structures:

David Borncamp, lead

Leonard Komow, assistant

Programming:

Leonard Komow, lead

Lauren Persons, assistant

Electrical:

Lauren Persons, lead

David Borncamp, assistant

Scientific:

Jonathan Pfohl, lead

Kirstyn Johnson, assistant

Biggest Worries

• Preventing bacterial death• Agar in a vacuum • Building the Geiger counter• Weight