Move over Stem Cells – Exosomes are Here! What are Exosomes & their role in Regenerative Medicine? Author/Presenter: Amartej Singh Deol MD4 Advisor: Shivinder S. Deol MD •MSC-Ex responsible for many beneficial physiological actions •Ex from Older Damaged or Cancer Cells cause expansion & progression of diseases including neurodegenerative diseases INGREDIENTS OF AN EXOSOME PHYSIOLOGICAL & PATHOLOGICAL FUNCTIONS WHAT CONDITIONS MAY EXOSOMES HELP? Cells releasing EVs (Exosomes & Microvesicles) EVs are secretions released from all cells in all bodily Əluids: •Saliva,blood, urine, milk, semen, sweat, tears, etc. EVs include: •Apoptotic Bodies or Blebs (ABs): Large cell fragments from cells undergoing apoptosis •Intraluminal Vesicles (ILVs): early endosomes invaginate within cell •Multivesicular Body (MVB): Derived when endosomes invaginate to ILVs •Microvesicles or Ectosomes: Larger particles 50-300 µm •Exosomes (Ex): Derived as biological vesicles originate as ILVs in MVBs. Released outside as EVs when MVB fuses with plasma membrane (bioactive chemicals released by human cells) EXTRACELLULAR VESICLES (EVs) •Ex are nanoparticles measuring only 30-150 nm •Ex are secreted by all cells including mesenchymal stem cells (MSC-Ex) •MSC-Ex carry Packets of Information including lipids, proteins, enzymes, micro & messenger RNA, & even DNA out of cells •MSC-Ex have multiple benefits including cell-to-cell communications, surveillance & immune functions •MSC mediate their inƏluence on distal tissues through paracrine secretions of multiple factors & messenger WHAT ARE EXOSOMES (Ex)? (carrier of many bioactive chemicals to target cells)

EXTRACELLULAR VESICLES (EVs)

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Move over Stem Cells – Exosomes are Here! What are Exosomes & their role in Regenerative Medicine?

Author/Presenter: Amartej Singh Deol MD4Advisor: Shivinder S. Deol MD

•MSC-Ex responsible for many beneficial physiological actions•Ex from Older Damaged or Cancer Cells cause expansion & progression ofdiseases including neurodegenerative diseases

INGREDIENTS OF AN EXOSOME

PHYSIOLOGICAL & PATHOLOGICAL FUNCTIONS

WHAT CONDITIONS MAY EXOSOMES HELP?

Cells releasing EVs (Exosomes & Microvesicles)

EVs are secretions released from all cells in all bodily �luids: •Saliva,blood, urine, milk, semen, sweat, tears, etc.EVs include:•Apoptotic Bodies or Blebs (ABs): Large cell fragments from cells undergoing apoptosis•Intraluminal Vesicles (ILVs): early endosomes invaginate within cell•Multivesicular Body (MVB): Derived when endosomes invaginate to ILVs•Microvesicles or Ectosomes: Larger particles 50-300 µm•Exosomes (Ex): Derived as biological vesicles originate as ILVs in MVBs. Releasedoutside as EVs when MVB fuses with plasma membrane

(bioactive chemicals released by human cells)

EXTRACELLULAR VESICLES (EVs)

•Ex are nanoparticles measuring only 30-150 nm•Ex are secreted by all cells including mesenchymal stem cells (MSC-Ex)•MSC-Ex carry Packets of Information including lipids, proteins, enzymes, micro &messenger RNA, & even DNA out of cells•MSC-Ex have multiple benefits including cell-to-cell communications, surveillance &immune functions•MSC mediate their in�luence on distal tissues through paracrine secretions ofmultiple factors & messenger

WHAT ARE EXOSOMES (Ex)?(carrier of many bioactive chemicals to target cells)