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Development of a mission-based national facility for microbial entomopathogens
National Chemical LaboratoryPune-411008
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PROFORMA – X
PROFORMA FOR SUBMISSION OF PROJECT PROPOSALS ON CREATIONOF
INFRASTRUCTURAL FACILITIES (To be filled by the applicant)
PART I: GENERAL INFORMATION
1. Name of the Institute/University/Organisation submitting the Project Proposal:National Chemical laboratory, Pune –411008
2. State: ..Maharashtra............................... 3. Status of the Institute: R&D (CSIR)(Please see Annexure-I)
4. Name and designation of the Executive Authority of the Institute/Universityforwarding the application: Director, National Chemical laboratory, Pune -411008
5. Project Title :........ Development of a mission-based national facility for microbialentomopathogens at NCIM Resource Centre, NCL
6. Specific Area (Please see Annexure - II): 1. AGRICULTURE & ALLIED AREAS(1.5 Biological Control of Plant Pests, Diseases and Weeds Biopesticides
7. Duration: ...Five........ Years
8. Total Cost (Rs.): 208.58 lakhs.
9. Project Summary (Not to exceed one page. Please use separate sheet).
Control of insect pests, particularly by their natural enemies comprising of
parasites, predators and microbes including fungi in agro-ecosystem is a continuous
process. Unlike bacteria and viruses, fungal agents act by contact and do not need
ingestion. Furthermore, fungi can be mass-produced very easily and are host-specific.
The genera such as Metarhizium, Beauveria, Verticillium, Nomuraea, Entomophthora,
Neozygites, etc. are commonly encountered in nature. Adequate development of
entomopathogenic fungi as biocontrol agents requires a selection schedule of species and
strains adapted to specific pests, environmental conditions and crops. Both biology and
physiology of entomopathogenic fungi depend on environmental parameters such as
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temperature and relative humidity or nutrient availability. For practical use of these
entomopathogens a sound knowledge of their behaviour in respect to environmental
factors is then of great significance. Having selected a virulent isolate for development as
a mycoinsecticide, an essential step is the maintenance of virulence and morphological
homogeneity. Many insect-pathogenic fungi are known to attenuate during successive
sub-culturing, resulting not only in a loss of virulence but also variation in conidiation
characteristics. The biochemical and molecular aspects of fungus-insect interaction,
importance of minimum sub-culturing and regular passage through insect host, molecular
markers for identification, horizontal transfer, etc., short and long term storage,
deposition in authentic culture collection center and maintenance in the natural habitat,
etc issues are with respect to the development of a commercially viable mycoinsecticide.
The mission-based culture collection can also provide a range of strains which have
different host specificities, virulent nature and different mechanisms for insect
interaction. Furthermore, such a facility can be a “single stop shop” for agriculture
researchers, entrepreneurs and practitioners too.
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PART II: PARTICULARS OF COORDINATORS (INVESTIGATORS)10. Name: .Dr.J.M.Khire.................................................................................
Date of Birth: .16-2-1955..........................................Sex (M/F): .M...................
Indicate whether Principal Investigator/Co-Investigator: Co-I
Designation :. Sci.F ....................................................................... Department :. NCIM Resource Center
Institute/University: . National Chemical Laboratory....................................................
Address:....Homi Bhabha Road., Pune-411008..................…………..
PIN: 411008
Telephone: 20 25902452........ Telex: ....Fax: 020 25902671........………………..........E-mail: [email protected]
11. Name:..Dr.M.V.Deshpande.............
Date of Birth: ..September 12, 1952..... Sex (M/F): ..M...................
Indicate whether Principal Investigator/Co-Investigator:. Principal Investigator
Designation:...Scientist G................................…………
Department:....Biochemical SciencesDivision........................................................................................................………..
Institute/University:..National Chemical Laboratory..............................
Address:....Homi Bhabha Road., Pune-411008.........................................…………..
PIN: 411008
Telephone: 20 25902246........ Telex: ....... Fax: 020 25902648...E-mail: [email protected].......
12. --
Note : Use separate page, if more investigators are involved
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PART III : TECHNICAL DETAILS OF PROJECT(Under the following heads on separate sheets)
13. Introduction (not to exceed 2 pages)
13.1 Origin of the proposal
Under the DBT and Swiss Agency for Development and Cooperation supportedproject (2000- 2010) on the use of entomopathogenic fungi to control Helicoverpaarmigera infested on pulses more than 100 isolates of Metarhizium, Beauveria andNomuraea were collected, identified, biochemically characterized, and tested in thelaboratory and selected were tested in field for their performance too. Specificmicrosatellite markers were developed to study persistence in soil, horizontal movement,if any. Nevertheless main concern is the virulence of isolates which has prompted us todevelop a mission mode culture collection, first of this kind in India.
The project was submitted to DBT as a “single stop shop” facility for agricultureresearchers, entrepreneurs and practitioners too in 2009. The then “Biopesticide and CropManagement” Task Force approved the concept, however, recommended to submitrevised proposal emphasizing repository and in vitro and in situ preservation aspects.
13.2 Rationale for the establishment of mission mode culture collectionfacility supported by cited literature
. Culture collections have long served as foci for biological science research. Theyserve a number of functions to support the advancement of research including:
the provision of uniform biological materials timely sharing of new materials maintenance of key materials under natural environment training and HRD for research groups quality and IPR maintenance of biotechnology industry Additionally gene libraries, cloned genes, cloning vectors, novel genes
from unculturable microbes
India has a long history of studying fungi and research with fungi which is beingcarried out in a different institutes. General culture collections in India include theNational Collection of Industrial Microorganisms (NCIM), NCL, Pune as well as thecollection of the 2,500 fungal strains in Indian Agricultural Research Institute, New Delhiand Microbial Type Culture Collection (MTCC), Chandigarh. In addition, the NationalBureau of Agriculturally Important Microorganisms have also been established.
A mission- based collection. This typically has a variety of strains whosebiological characteristics have been cataloged without carrying out traditional geneticson the strains.
The advantages of mission mode culture collection, however supplyingcollaborators worldwide with isolates of fungi presents unique challenges. One of the
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mission mode collections is of USDA-ARS, USA. The ARS Entomopathogenic Fungalculture collection is the world's largest and most diverse collection of cultures for fungalpathogens of insects, mites, spiders, nematodes, and other invertebrates. The collectioncurrently comprises nearly 6000 isolates from more than 400 fungal taxa and ca. 930diverse hosts, with isolates coming from every continent of the globe. No other culturecollection offers either such diversity or depth of holdings as does the ARSEF collection.
In addition to the preservation of entomopathogens as mycosed insects reared inthe laboratory, the preservation of organisms in their natural environment is one ofthe novel features especially for the mycoparasitic and insect pathogenic fungi whichthus can maintain their virulent nature for further exploitation.
Control of insect pests, particularly by their natural enemies comprising ofparasites, predators and microbes including fungi in agro-ecosystem is a continuousprocess. Unlike bacteria and viruses, fungal agents act by contact and do not needingestion. Furthermore, fungi can be mass-produced very easily and are host-specific.The genera such as Metarhizium, Beauveria, Verticillium, Nomuraea, Entomophthora,Neozygites, etc. are commonly encountered in nature. Adequate development ofentomopathogenic fungi as biocontrol agents requires- a selection schedule of species and strains adapted to specific pests,
environmental conditions and crops. Both biology and physiology of entomopathogenic fungi depend on
environmental parameters such as temperature and relative humidity or nutrientavailability.
For practical use of these entomopathogens a sound knowledge of their behaviourin respect to environmental factors is then of great significance.
Having selected a virulent isolate for development as a mycoinsecticide, anessential step is the maintenance of virulence and morphological homogeneity.Many insect-pathogenic fungi are known to attenuate during successive sub-culturing, resulting not only in a loss of virulence but also variation in conidiationcharacteristics (Nahar et al 2008).
The biochemical and molecular aspects of fungus-insect interaction, importanceof minimum sub-culturing and regular passage through insect host, short andlong storage, deposition in authentic culture collection center and maintenance inthe natural habitat, etc issues are important with respect to the development of acommercially viable mycoinsecticide.
AimTo establish a “state of the art” facility for entomopathogens catering to all the aspects ofmission based collection, in vitro and in situ maintenance, field performance studies ofselected isolates and training under one roof
Present situation•Non availability of the focussed collection of organisms for research laboratories andagriculture practitioners•No facility for the evaluation of the biocontrol agents on different pests
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•No integrated training facility for isolation and characterization•No facility for the maintenance of entomopathogens in their natural habitats
14. Specific objectives
In house isolation programme of novel entomopathogens/ mycoparasites/ dualpathogens
Researchers can have facilities for patent deposits (IDA under Budapest Treaty) Extension of the facility of maintenance, preservation and distribution of
entamopathogens to research organizations and industries Biochemical and molecular characterization of the entomopathogens/
mycoparasites/ dual pathogens Development of biochemical and molecular markers for persistence, horizontal
transfer studies and for strain protection Maintenance of biocontrol organisms in their natural environment
15. Previous experience in the field
15.1. Work already done by the Department/Institute in this field (supported bypapers published and grants received in the field)
Collection of different isolates
At present Co-PI has collection of different insect pathogenic fungi and bacteriawhich show antagonism against plant pathogenic fungi. For instance insectpathogenic fungi of the genera Metarhizium (63), Nomuraea (15), Beauveria (7),Others (not yet identified) (16) and 105 bacterial isolates screened for theirantagonistic behaviour, CDE/ME production, and production of chitin metabolisminhibitors. The tentative identification of bacterial isolates is: 26 Bacillus sp., 4Actinomycetes sp, and 4 Pseudomonas sp., remaining isolates are beingidentified. The organisms involved in biocontrol as well as in the bioremediationof pesticide residues are also being studied.
The 3 rd group of organisms is of plant pathogenic fungi. We are working onSclerotium rolfsii (ground nut); Rhizoctonia solani (Potato, Rice); Drechsleraoryzae (Rice); Fusarium oxysporum (Cotton, Tomato); Magnaporthe grisea(Rice); Colletotrichum capsici (Capsicum); Pythium sp (Papaya).
One of the important methods to identify target specific molecules for thebiocontrol is haploinsufficiency assay. This has been standardized usingSaccharomyces mutants for different targets.
Study of microbial diversity of the fields involved in bioremediation of oil spillsand for potential biocontrol agents.
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The fungi (143) isolated from grapes and involved in the wine spoilage are beingstudied to understand the storage and market diseases and to design controlstrategies.
Maintenance and preservation
PI, is working as a scientist in National Collection of Industrial Microorganisms(NCIM), since 1981 and maintaining most of the bacterial and fungal cultures andstudy their performances regularly.
In addition to regular subculture, all the cultures are maintained by glycerolstocks, paraffin oil overlay method, soil cultures and lyophilization.
At present we are maintaining entamopathogenic fungi by regular subculturingand storage at -80 C as glycerol stocks and also as mycosed insects.
All the cultures are being maintained by regular subculturing and by storage asglycerol stocks, and lyophilized cultures. The purity and viability as well asgrowth tests – specific metabolites, cell wall analysis, extra-cellular enzymeprofiling and molecular markers are being carried out regularly.
Facility for the collection and rearing of insects
The Co-PI has already established the facility to collect insects from the field forbioassays and their maintenance. These include, Helicoverpa, Spodoptera, mealy bug,woolly aphids, rice moth, Galleria to name a few. The rearing of Helicoverpa armigera,Spodoptera litura, and mealy bugs is being carried out.
Laboratory to market
Co-I has already established a ‘proof of concept’ with different isolates
Human resource development
Training of researchers and agriculture practitioners and farmers’ education wereimportant aspects with earlier DBT programme with Co-I.
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LIST OF BIOCONTROL PUBLICATIONS from P-I and Co-I (2011)
Books
Deshpande M.V. (1998) Novel biopesticides. A popular science book. National Instituteof Science Communication, New Delhi.
Deshpande M.V. (2004) Entomopathogenic fungi- a valuable alternative to fightagainst insect pests. A Proceeding of the International Workshop organized at NCL,Pune in Sept. 2002.
Research papers
1.Kumar A., Kulye M., Deshpande M.V. and Rao M. (2010) The inhibition of moltingfluid enzymes of Pod borer, Helicoverpa armigera (Hubner) by an aspartic proteaseinhibitor: Its effects on insect development. Entomol. Res. 40: 113–121
2.Chavan S., Kolomiets E., Kuptsov V., Mandrik M., Kulkarni M. and Deshpande M.V.(2009)Significance of cuticle degrading enzymes with special reference to lipase inbiocontrol of sugarcane woolly aphids. J. Mycol. Plant Pathol. 39:118-123
3.Chaudhary P.M., Chavan S.R., Shirazi F., Razdan M., Nimkar P., Maybhate S.P.,Likhite A.P., Gonnade R., Hazara B.G., Deshpande M.V., and Deshpande S.R. (2009)Exploration of click reaction for the synthesis of modified nucleosides as chitin synthaseinhibitors. Bioorg. Med. Chem. 17: 2433-2440 (IF 2.662)
4.Kulkarni S.A., Ghormade V., Kulkarni G., Kapoor M, Chavan S.B., Rajendran A., PatilS.K., Shouche Y., and Deshpande M.V. (2008)Comparison of Metarhizium isolates forbiocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae) in chickpea. BiocontrolSci. Technol. 18: 809-828. (IF 1.087)
5.Nahar P.B., Kulkarni S.A., Kulye M.S., Chavan S.B.,Kulkarni G., Rajendran A.,YadavP.D., Shouche Y. and Deshpande M.V. (2008) Effect of repeated in vitro sub-culturingon the virulence of Metarhizium anisopliae against Helicoverpa armigera (Lepidoptera:Noctuidae). Biocontrol Sci. Technol.18: 337-355 .
6.Gholap A.R., Toti K.S., Shirazi F., Ratna Kumari, Bhat M.K., Deshpande M.V. andSrinivasan K.V. (2007) Synthesis and evaluation of antifungal properties of a series ofthe novel 2-amino-5-oxo-4-phenyl-5, 6, 7, 8-tetrahydroquinoline-3-carbonitrile and itsanalogues. Bioorg. Medicinal Chem. 15:6705-6715
7.Shirazi F., Kulkarni M. and Deshpande M.V. (2007) A rapid and sensitive method forscreening of chitinase inhibitors using Ostazin Brilliant Red labeled chitin as a substratefor chitinase assay. Lett. Appl. Microbiol. 44:660-665.
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8. Salunke D.B., Hazra B.G., Pore V.S., Bhat M.K., Nahar P.B. and Deshpande M.V.(2004) New steroidal dimers with antifungal and antiproliferative activity. J. Med. Chem.47: 1591-1594
9. Nahar P.B., Ghormade V. and Deshpande M.V. (2004) The extracellular constitutiveproduction of chitin deacetylase in Metarhizium ansiopliae: Possible edge to entomo-pathogenic fungi in the bio-control of insect pest. J. Invertebr. Pathol. 85: 80-88(Featured in Newsindia, a Nature's science & policy round-up for India, June 2004).
10. Nahar P.B., Kulye M., Yadav P., Hassani M., Tuor U., Keller S. and DeshpandeM.V. (2003) Comparative evaluation of indigenous fungal isolates, Metarhiziumanisopliae M34412, Beauveria bassiana B3301 and Nomuraea rileyi N812 for thecontrol of Helicoverpa armigera (Hüb.) on chickpea. Indian J. Mycol. Plant Pathol. 33:372-377.
11. Nahar P.B., Yadav P., Kulye M., Hadapad A., Hassani M., Tuor U., Keller S.Chandele A., Thomas B. and Deshpande M.V. (2004) Evaluation of indigenous fungalisolates, Metarhizium anisopliae M34412, Beauveria bassiana B3301 and Nomuraearileyi N812 for the control of Helicoverpa armigera (Hübner) in pigeon pea field. J. Biol.Control 18:1-7
12 Patil R.S., Deshpande A.M., Natu A.A, Nahar P., Chitnis M., Ghormade V.,Laxman R.S., Rokade S. and Deshpande M.V. (2001) Biocontrol of root infectingplant pathogenic fungus, Sclerotium rolfsii using mycolytic enzymes and chitinmetabolism inhibitors singly and in combination. J. Biol. Control . 15:157-164
13. Mendonsa E. S., Vartak P. H., Rao J. U. and Deshpande M. V. (1996) An enzymefrom Myrothecium verrucaria that degrades insect cuticle for biocontrol of Aedes aegyptimosquito. Biotechnol. Lett. 18: 373-376.
14. Vyas P. R. and Deshpande M. V. (1993) Purification and characterization of anendochitinase from Myrothecium verrucaria. J. Gen. Appl. Microbiol. 39: 91-99.
15. Vyas P. R. and Deshpande M. V. (1989) Chitinase production by Myrotheciumverrucaria and its significance for fungal mycelia degradation. J.Gen.Appl.Microbiol.35: 343-350.
Reviews
1.Ghormade V., Deshpande M.V. and Paknikar K.M (2011) Perspectives for nano-biotechnology in plant protection and nutrition. Biotechnol. Adv. 29: 792–803 (IF 8.25)
2.Yadav P and Deshpande M.V. (2010) Fungus- fungus and fungus–insect interactions.Biopestic. Int. 6:21-35
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3. Patil R.S., Ghormade V. and Deshpande M.V. (2000) Chitinolytic enzymes: Anexploration. Enzyme Microbial Technol. 26 473-483.
4. Deshpande M.V. (1999) Mycopesticide production by fermentation:Potential and challenges. Critical Reviews in Microbiology 25: 229-243.
5. Deshpande M. V. (1996) The effect of morphological changes in fungalpathogenesis. Indian J. Med. Microbiol. 14: 123-131.
6. Shaikh S. A. and Deshpande M. V. (1993) Chitinolytic enzymes: Their contributionin basic and applied research. World J. Microbiol. Biotechnol. 9: 468-475.
7. Deshpande M. V. (1992) Proteinases in fungal morphogenesis. World J. Microbiol.Biotechnol. 8: 242-250.
8. Deshpande M. V. (1986) Enzymatic degradation of chitin and its biologicalapplications; J. Sci. & Ind. Res. 45: 273-281.
Chapters in Books/ Proceedings
1.Ghormade V., Kulkarni S., Doiphode N., Rajamohanan P.R. and Deshpande M.V.(2010) Chitin deacetylase: A comprehensive account on its role in nature and itsbiotechnological applications. Current Research, Technology and Education Topics inApplied Microbiology and Microbial Biotechnology (A. Mendez-Vilas, ed) pp.1054-1066, Formatex Research Center, Badajoz, Spain
2. Chavan S., Kulkarni M. and Deshpande M. V.(2008) Status of microbial pesticides inIndia In: Review of Plant Pathology Vol. 4 (Ed. Prof. S. M. Reddy and H.N. Gour)pp.393-420, Sci. Publishers, Jodhpur
3. Deshpande M.V. (2005) Formulations and applications of mycopathogens. In:Microbial biopesticide formulations and application. Tech.Document No. 55. Eds.R.J.Rabindra, S.S.Hussaini and B.Ramanujam. pp.150-158, PDBC, Bangalore.
2. Deshpande M.V. (2005) Chitosan production by fungi. In: Chitin and chitosan,opportunities and challenges. Ed. P.K.Dutta, pp.59-68, SSM International Publication,Contai, India.
4. Chavan S. , Ghormade V. , Nahar P. and Deshpande M.V. (2005) Entomopathogenicfungi: A valuable tool to fight against insect pests. In: Plant Protection for the NewMillennium. Eds. Ashok V Gadewar, B P Singh and I D Garg, pp.815-830, Satish SerialPublishing House, New Delhi
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5* Chavan S., Kulye M., Kulkarni M. and Deshpande M.V. Myrothecium verrucaria:A potential biocontrol agent for the control of insect pest. In: Proceedings of the NationalSymposium of biomanagement of Insect Pests, Annamalainagar, 2003 [In Press].
6. Deshpande M.V. (2005) Mycopesticides: Their potential and challenges In :Fungi:Diversity and Biotechnology. Ed. M.K.Rai and S.K.Deshmukh, pp.375-390.Scientific Publishers, Rajasthan.
7. Hassani M., Tuor U., Deshpande M., Hadapad A., Nahar P., Bucher T., Kulye M.,Yadav P., Enkerli J. and Keller S. (2004) Mass production of entomogenous fungusMetarhizium anisopliae (Deuteromycotina: Hyphomycetes) for biological control ofhelicoverpa armigera (Lepidoptera: Noctuidae)on pulses. In: Proceeding of theinternational workshop on entomopathogenic fungi - a valuable alternative to fightagainst insect pests ( September 16-18, 2002). Ed. M.V.Deshpande, pp. 37-49, NationalChemical Laboratory, Pune, India.
8. Deshpande M.V., Keller S., Hassani M., Chandele A., Nahar P., Hadapad A., KulyeM., Yadav P. Bucher T. and Tuor U. (2004) Comparative evaluation of indigenous fungalisolates , Metarhizium anisopliae M34412, Beauveria bassiana B3301 and Nomuraearileyi N812 for the control of Helicoverpa armigera (Hüb.) on pulses. In: Proceeding ofthe international workshop on entomopathogenic fungi - a valuable alternative to fightagainst insect pests ( September 16-18, 2002). Ed. M.V.Deshpande, pp. 51-59, NationalChemical Laboratory, Pune, India.
9. M. V. Deshpande, A.G. Chandele, P. Nahar, A. Hadapad, G. Patil, V. Ghormade,S.Keller, and U. Tuor (2003) Entomopathogenic fungi: Mycoinsecticides useful againstlepidopteran pest in pulses... IOBC/WPRS Bull. 26: 27-30.
10. Natu A.A. and Deshpande M.V. (2003) Combinatorial chemistry and highthroughput screening: a tool for the faster discovery of fungicides. Proceedings of the IVAsia Pacific Crop Protection Conference-2003. Pesticides Manufacturers & FormulatorsAssociation of India, Mumbai. pp. .
11. Deshpande M.V. (2002) Fungi and fungal products in insect pest management:Entomopathogenic fungi as mycoinsecticides. In: Proceedings of National trainingCourse on Production and use of bio-agents in plant protection in agriculture. pp. 110-117.
12. Deshpande M.V. and Tuor U. (2001) Microbial control of pests: Entomopathogenicfungi as mycoinsecticides. Proceedings of the III Asia Pacific Crop ProtectionConference-2001. Pesticides Manufacturers & Formulators Association of India,Mumbai.pp. 56-59.
13. Nahar P., Patil A.S., Gooday G.W., Ghormade V. and Deshpande M.V. (2001)Theconstitutive production of chitin deacetylase and chitosanase activities in Metarhiziumanisopliae: Possible role in the biocontrol of insect pest. In: Proc. 3rd International
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Symposium on Chitin Enzymology (R.A.A.Muzzarelli, ed.) European Chitin Society,Ancona, Italy, pp.511-519 [Peer Reviewed].
14. Ghormade V. and Deshpande M.V.(2000) Dimorphic germination of spores and itssignificance in designing biocontrol strategies. Nat. Bot. Soc. 54:19-23 [Peer Reviewed].
15. Deshpande M.V. (2000) Mycopesticide production: Contribution of fungalmorphologies in the biopesticide formulations. Nat. Bot. Soc. 55:11-17 [Peer Reviewed].
16. Deshpande M.V. (1999) Mycopesticide production: Potential andchallenges. In: Proc. National Seminar on Advances in Plant Pathology, University ofPune, Pune. pp. 21-30.
17. Deshpande M.V. (1998) Biochemical basis of fungal differentation. In: Wealth andSustainable Environment (A.Varma, ed) 241-252, Malhotra Publishing House, NewDelhi.
18. Deshpande M.V. (1998) Chitin metabolism: A target for antifungal and insecticidalagents. In: Wealth and Sustainable Environment (A.Varma, ed) 281-291, MalhotraPublishing House, New Delhi.
19. Deshpande M.V. (1998) Cuticle degrading and mycolytic enzymes withspecial reference to chitinases as biopesticides: status, potential and challenges. In:Symposium on Biotechnology- a challenge for 2005. All India Biotech Association, NewDelhi. pp.1-4.
20. Deshpande M.V. (1998) Biopesticide production by fermentation: Scope andlimitations. In: Microbial Pesticides & Insect Pest Management (H.D.Rananavare,S.R.Naik and T.K.Dongre, eds) 75-80, BARC, Mumbai and HAL, Pune
21. Deshpande M.V. (1998) Cuticle degrading and mycolytic enzymes with specialreference to chitinases as biopesticides: status, potential and challenges. In: MicrobialPesticides & Insect Pest Management (H.D.Rananavare, S.R.Naik and T.K.Dongre, eds)142-146, BARC, Mumbai and HAL, Pune
22. Deshpande M.V. (1997) Novel molecules for biocontrol of plant-pathogenic fungiand insects. Rayat Res. J. 5: 83-95 (Published in 1998).
Report
1. Chidambaranathan, Soraya Verjee, Smita Premchander and M.V. Deshpande. 2004.Assessment of an Indo-Swiss Research Partnership Project: Microbial Control of InsectPest: Entomopathogenic Fungi as Mycoinsecticides, A Southern Perspective, to be put onwebsite of KFPE, Berne and incorporated in elaborating guidelines for north southresearch and development partnerships. www.sampark.org/articles.htm
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Patents
1.A method for preparation of mycolytic enzymes containing mainly chitinase.(M.Deshpande, M. Desai, M.Chitnis, and R.Patil). Indian patent filed (March 2001).
2.A process for the preparation of mycolytic enzymes containing mainly chitosanase(M.Deshpande, R.Patil, M.Chitnis, and R.Seeta Laxman). Indian patent 193300 datedJuly 17, 2004
3.New steroidal dimers with antifungal and antiproliferative activity (Salunke D.B.,Hazra B.G., Pore V.S., Bhat M.K., Nahar P.B. and Deshpande M.V.). US patent applied(February 2004)
4. New substituted 1,4-dioxa-8-azaspiro(4,5)decane compounds used as fungicide forwide range of fungi such as Pyricularia oryazae, Rhizoctonia solani, Pythiumaphanidematum, Dreshlera oryazae, Collatrotricum capsicum, and Fusarium oxysporum(Deshpande M V, Deshpande S R, Shirazi F, Chaudhary P M, Rao N M, Mohanty B,Sharma N N, Bachhawat A K, Kaliannan G, Paul S, Kumar R, Rao B V, Gawali B B,Reddy V V N, Yadav J S, Nelavelli M R, Raj K, Bommena V R, Vaddu V N R) PatentNumber(s).:WO2010109299-A2; WO2010109299-A3; IN200900612-I1
5. A composition for controlling woolly aphid infestation and a method of treatment(M.Deshpande, M.Kulkarni and S. Chavan) Indian patent filed (January 2004)
Technical brochures
1. For a DBT sponsored project "Development of Biocontrol Agent using Chitin as aTarget", a technical brochure was released by NCL, Pune, September 2001.
2. For a Indo-Swiss Collaboration in Biotechnology (ISCB) project “Microbial control ofpests: Entomopathogenic fungi as mycoinsecticides” published a Technical InformationBrochure of the project entitled: “Mycoinsecticides New Products in the fight againstHelicoverpa armigera Pest in Pulses” in September 2002
Popularisation of Biopesticides
The farmers meetings are organised before field demonstrations with the help ofAgric. Universities and NGOs for their education and the promotion of eco-friendly organic farming.
The 3 credit course for M.Sc. Microbiology on biopesticides in Pune University(2005-06)
Lectures in Ornithology course of Pune University. The role of birds asenvironment indicators and as a component in Integrated Pest Management.
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Research projects undertaken (National and International):
On-going
1.Development of sustainable pest management strategies basedon entomopathogenicfungi and cuticle degrading enzyme complex against mealy bug, Maconellicoccushirsutus (Green) on grapes in India (April2009- March2012, DBT,New Delhi)
2.Development of environmentally benign nanomaterial-based enzyme formulations forbiocontrol of plant pathogens and pests (Nov.2009- Oct. 2012, DBT, New Delhi)
3.Transferring mycoinsecticide technology to the private sector (Nov.2009- March 2011,Indo-Swiss Collaborative programme of DBT, New Delhi and SDC, Berne, Switzerland;SBIRI project approved)
*****1. Development of a mycoinsecticide against Helicoverpa armigera in pluses: from thelaboratory to the market (September 2004- march 2008, Indo-Swiss Collaborativeprogramme of DBT, New Delhi)
2. Exploration & exploitation of microbial wealth of India for novel compounds andbiotransformation processes (c) Chitosan production by fermentation using the fungusBenjaminiella poitrasii & applications in chitosan-based drug carriers and human healthcare items (April2003-March 2006; CSIR Network project)
3. Development of novel fungicides (Oct-2004- September 2007; CSIR NMITLIprogramme).
4. Fungi and fungal enzymes to combat the white woolly aphid menance on sugarcane(January 2004- December 2005; CSIR, New Delhi)
5.Scale-up, field demonstration and techno-economics of enzyme complex fromMyrothecium verrucaria as a biocontrol agent (June 2003- May 2006; DBT, New Delhi)
7.Development of novel biopesticides based on fungi/ bacteria exhibiting dualantagonistic activity against plant pathogenic fungal and insect pests (April 2002-March2005; Indo-Belarus Collaboration in Biotechnology, DBT, New Delhi)
8.Microbial control of pests:Entomopathogenic fungi as mycoinsecticides"(April 2000-March 2004; Indo-Swiss Collaborative programme)
9.Development of biocontrol agents for the control of plant pathogenic fungi and insectsusing chitin as a target (August 1997- July 2000; DBT, New Delhi)
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10.Biological control agents: Chitin as a target for control of pathogens and pests inagriculture and medicine (April 1996- March 2000; Higher Education Link Programmeof British Council)
11.Significance of chitinase/chitin synthetase complex in fungal differentiation andstudies on its role in plant, fungus and insect interaction (June 1993- May 1997; DST,New Delhi)
15.2. Whether the PI/Co-I have previous track record of running such facilityearlier? If yes…
P-I, is working as a scientist in National Collection of Industrial Microorganisms(NCIM), since 1981 and maintaining most of the bacterial and fungal cultures andstudy their performances regularly.
By training PI is microbiologist and actively collaborates with National Collectionof Industrial Microorganisms group and with industry for proof of concept tocommercialization activities.
Is routinely maintaining and preserving variety of isolates (400 plus) whichinclude: insect pathogenic fungi, bacteria, plant pathogens, wine spoilage yeasts,and other.
PI has developed different bioassays for antifungal agents for screening.
Actively involved in a strain improvement programme for hyper-cellulaseproducers (Sclerotium rolfsii, Penicillium funiculosum); protease minus mutant(Conidiobolus coronatus); form -specific monomorphic mutants (Benjaminiellapoitrasii); ura minus mutant (Yarrowia lipolytica).
Evaluation of the microbial resources in the states of Maharashtra, Gujarat and Rajasthan (April 2001-March 2002, DBT, New Delhi).
Exploration & exploitation of microbial wealth of India for novel compounds andbiotransformation processes (c) Chitosan production by fermentation using thefungus Benjaminiella poitrasii & applications in chitosan-based drug carriers andhuman health care items (April2003-March 2006; CSIR Network project).
16. Status of the other investigators of the cluster (within and outside the institute)
As mentioned under 15.2
17
17. List of prospective users of the facility
Three workshops were organized in NCL to identify potential users for the said facility.
International Workshop Organised a workshop on Entomopathogenic fungi- avaluable alternative to fight against insect pests at National Chemical Laboratory,Pune, India, from September 16-18,2002 (DBT and SDC support)
Seed industry meet at National Chemical Laboratory, September 22, 2005 ( NCL)
Public Private partnership in plant protection for sustainable agriculture meetingorganized at National Chemical Laboratory, Pune, India from May 12-13, 2006 (DBT;supported by Biopesticide Task Force)
The list of potential users is as follows:
Mrs. Vasudha Sardar (NGO)Nava Nirman NyasPargaon (Aalo Malo)Tal. Daund- 412 203
Dr. C. S. Pawar (NGO)Shree Vivekanand Research and TrainingInstitute103 Rajashree Apt. 7-1-29/4, AmeerpetHyderabad – 500 016.
Bafna Farm Krishi Udyog,At Post : Pathethan,Devkarwadi Phata, Pune Rahu Road,Tal. : Daund, Dist. : Pune
Maharashtra State Grape GrowersAssociation‘Draksha Bhavan’, E/4,Market yard, Pune – 411 037
Bio-Control Research Laboratories,A division of Pest Control India Ltd.P. O. Box 3228, RT Nagar,Bangalore 560 032, INDIA
HiTech Bio Sciences Pvt.Ltd,S.No.297/298, Plot 6&8, Sutarwadi, PostPaud, Gadadawane Road, Behind DuttaMandir, tal.Mulshi, Pune-412108.
Development ExecutiveSyngenta India Ltd.Seeds DivisionR&D Centre, Gat No. 2347Wagholi, Pune-Nagar RoadPune 412 207, INDIA
Rallis India LimitedRallis Research Centre21 & 22 Peenya Industrial Area Phase IIBangalore 560 058, INDIA
BIOTECH International Ltd.“VIPPS CENTRE”, 2, Local ShoppingCentre,Block – EFGH, Masjid Moth,Greater Kailash –IINew Delhi – 110 048
Sri Biotech,112, Sree Arcade, Bata Complex,Erragadda, Hyderabad – 500 018
Biotech DivisionNirmal Seeds Pvt. Ltd.A-2, Vallab Apartment, Sejal Park, LinkRoad,Goregaon (W),Mumbai – 400 014. India
Jay Biotech32, Market Yard, Gultekdi,Pune – 411 037
18
18. Timelines:
Period of study Achievable targets
12 Months Recruitment of manpower, purchase and installation ofequipment, training of manpower,
24 Months Standardization of preservation techniques and other protocols;biochemical and molecular identification of existing isolates;identification of biochemical and molecular markers, ESTs forthe protection of the strain and other studies; Documentationfor IPR related issues; Collection of biocontrol organisms
36 Months Some of the activities continued: Biochemical and molecularidentification; identification of biochemical and molecularmarkers for the protection of the strain and other studies;collection of biocontrol organisms; field studies; preservationin the natural environment
48 Months Maintenance of the strains; functional genomics studies usingchitinase, protease and other genes; proof of concept tocommercialization studies; field studies; preservation in thenatural environment
60 Months field studies for selected isolates for non-target effects, etc.;preservation in the natural environment
19
Novel approaches- Expressed Sequence Tags for the important strains; functionalgenomics; preservation in the natural environment; liaison between researchers andindustry
Trans sectoral issues including farmers’ education, awareness, do it yourselfstrategies etc. will be implemented with the help of NGO’s, Agric. Universities andothers. The involvement of media will be one of the features for these issues.
19. Timelines for making the facility financially viable
Culture collections perform an important job of preserving biodiversity. They canbe partially self sustained. Culture characterization and maintenance form important partof activities and also because large portions of collections are rarely requested, adequaterevenues can not be earned through fees and services. However considering the fact thatsuch collections
1. serve important national interest2. necessary for the purpose of health, education, commerce3. deposition of cultures at such collections is requirement for scientific publications
and for patents and hence scientific community (academic as well as industrial)depend on such collections, it is important to assure continuous and sustainedfunding for such collections.
20. List of 5 experts in India in the proposed subject area
Sr.No.
Name Designation
Address
1 Dr. T.Satyanarayana Professor Dept. of MicrobiologyUniversity of Delhi SouthCampusBenito Juarez Road, [email protected]: 01124112008
2 Dr. Appa Rao Podile Professor Dept. Plant SciencesUniversity of HyderabadHyderabad 500 [email protected]: 040-23134503
3 Dr. Rajendra Prasad Professor School of Life SciencesJawaharlal Nehru UniversityNew Delhi-110067Tel.: 0091 11 26717676 Ext.2509E-mail: rajendra [email protected]
20
4 Dr. K.P. Jayanth Researchscientist
Bio-Control ResearchLaboratories,A division of Pest ControlIndia Ltd.P. O. Box 3228, RT Nagar,Bangalore 560 032, INDIATel +91 80 8468839-43Fax [email protected]@vsnl.com
5 Dr. K.R.K.Reddy Director Sri Biotech,112, Sree Arcade, BataComplex,Erragadda, Hyderabad – 500018Phone: - 040-23702823/23701153e-mail : [email protected]
21
PART IV : BUDGET PARTICULARS(In Rupees)
A. Non-Recurring (e.g. equipments, accessories, etc.)S.No.
Item Year 1 Year 2 Year 3 Year 4 Year 5 Total
1
2
3
4
5
6
InfrastructureInsect rearing
Glass house1000 sq.ft withmaintenancefacilityFreeze dryer
Thermocyclar
Incubators (3)
Laminar hoods
4.00
-----
10.00
4.00
2.00
3.00
Nil
Nil
Nil
Nil
Nil
Nil
Nil
Nil
Nil
Nil
Nil
Nil
Nil
30.00
Nil
Nil
Nil
Nil
Nil
Nil
Nil
Nil
Nil
Nil
4.00
30.00
10.00
4.00
2.00
3.00
Sub-Total (A) Rs. 53 lakhs
B. Recurring
B.1 Manpower (See guidelines at Annexure-III)
S.No.
PositionNo.
ConsolidatedEmolument
Year 1 Year 2 Year 3 Year 4 Year 5 Total
RA (1)
JRF (2)
Rs. 20000/m3rd Yr onwardRs. 25000/mRs. 12000/m3rd Yr onwardRs. 16000/m
3.6
4.32
3.6
4.32
4.5
5.76
4.5
5.76
4.5
5.76
20.70
25.92
Sub-Total (B.1) = Rs. 46.62 lakhs
22
B.2 ConsumablesItem Year 1 Year 2 Year 3 Year 4 Year 5 Total
Chemicals andglassware, plasticware for the growthand preservation ofcultures. Growthmedia,preservatives,Reagents formolecular andbiochemicalstudies; chemicalsfor glass housestudies
10.00 10.00 10.00 20.00 20.00 70.00
Sub-Total (B.2) =Rs. 70 lakhs
Other items Year 1 Year 2 Year 3 Year 4 Year 5 TotalB.3 Travel 1.00 1.00 1.00 1.00 1.00 5.00
B.4 Contingencies for dataprocessing, access to climaticdata, field trials, patentand IPR related issues, trans-sectoral issues
1.00 1.00 1.00 1.00 1.00 5.00
B.5 Overhead Charges 2.98 2.98 3.34 4.83 4.83 18.96
B.6 Workshop/Training* - - - 10.00 - 10.00
Sub-Total (B = B.1 + B.2 +B.3 + B.4 + B.5 + B.6)
22.90 22.90 25.60 47.09 37.09 155.58
Grand Total (A + B) 45.90 22.90 25.59 77.09 37.09 208.57
Note: Please give justification for each head and sub-head separately mentioned in theabove table. (Financial Year: April – March)* The grant for workshop/training will be released only after submission of a detailedproposal indicating different heads of the budget, participants, duration of training etc.
23
PART V : EXISTING FACILITIES
Information on existing resources such as laboratory, manpower, equipments, and otherinfrastructure facilities.
1. Workshop- Yes 2. Water and Electricity- Yes
3. Standby power supply- Yes 4. Air-conditioned room- Yes
5. Laboratory space and Furniture- Yes 6. Transportation- Yes
7. Telecommunication- Yes 8. Admin. Staff- Yes
9. Library- Yes 10.Cold room- Yes
11.Shaker room- Yes 12.Computers-Yes
Instruments
UV/visible and fluorescence spectrophotometer, Rotavapor unit, electrophoresis unit,brightfield and fluorescence microscope, Inverted microscope, isoelectric focusing unit,high speed centrifuge, ultracentrifuge, lyophilizer, incubation shakers, SEM, HPLC, GC,thermocyclar, RT-PCR, Gel documentation system, fermenter, etc.
Culture collection facilities which can be shared
1. Lyophilizer2. Fermenter3. HPLC4. GC5. Deep freeze6. Cold room7. Sonicator8. Shaker incubator
24
PART VI : DECLARATION/CERTIFICATIONIt is certified that
a) the same project has not been submitted to any other agency/agencies for financialsupport.
b) the emoluments for the manpower proposed are those admissible to persons ofcorresponding status employed in the institute/university or as per the Ministry ofScience & Technology guidelines (Annexure-III)
c) necessary provision for the scheme/project will be made in theInstitute/University/State budget in anticipation of the sanction of the scheme/project.
d) if the project involves the utilization of genetically engineered organism, it is agreedthat we will ensure that an application will be submitted through our InstitutionalBiosafety Committee and we will declare that while conducting experiments, theBiosafety Guidelines of the Department of Biotechnology would be followed in toto.
e) if the project involves field trials/experiments/exchange of specimens, etc. we willensure that ethical clearances would be taken from concerned ethicalCommittees/Competent authorities and the same would be conveyed to theDepartment of Biotechnology before implementing the project.
f) it is agreed that any research outcome or intellectual property right(s) on theinvention(s) arising out of the project shall be taken in accordance with theinstructions issued with the approval of the Ministry of Finance, Department ofExpenditure, as contained in Annexure-V.
g) we agree to accept the terms and conditions as enclosed in Annexure-IV. The same issigned and enclosed.
h) the institute/university agrees that the equipment, other basic facilities and such otheradministrative facilities as per terms and conditions of the grant will be extended toinvestigator(s) throughout the duration of the project.
i) the Institute assumes to undertake the financial and other management responsibilitiesof the project.
Signature of Executive Authority(of Institute/University with sealDate :
Signature of Principal InvestigatorDate :
Signature of Co-InvestigatorDate :
25
PART VII : PROFORMA FOR BIOGRAPHICAL SKETCH OFINVESTIGATORS (COORDINATORS)
Provide the following information for the key personnel in the order listed on PART II.Follow this format for each person. DO NOT EXCEED THREE PAGES
Bio-data
Name : Dr. J. M. KhireDesignation : Scientist FDepartment : NCIM Resource Center Division of Biochemical Sciences
National Chemical Laboratory Pune 411 008
Institute : National Chemical Laboratory, Pune 411 008Address : Division of Biochemical Sciences, National Chemical
Laboratory, Dr. Homi Bhabha Road, Pune 411 008Telephone : 020-2590 2452 Fax: 020-2590 2671E mail : [email protected] of Birth : 16.2.1955
Recognition as Post-graduate teacher for M. Phil. and Ph.D. in Microbiology andBiotechnology, Pune University.
Academic Qualifications
Sr.No.
Institute/Place Degree awarded Year
1 University of Pennsylvania,Philadelphia, USA
Post-doctoral researchFellow
1988-1990
2 University of Pune Ph. D. (Biochemistry) 19873 University of Pune(ARI, Pune) M. Sc. (Microbiology) 19784 University of Pune (Garware College) B. Sc. (Microbiology) 1975
Ph. D. thesis title: Studies on Extracellular, specific D-xylose isomerase fromChiania sp.
Research Specialization: Microbiology, Fermentation Technology, Strainimprovement, Biochemistry (Enzyme chemistry-Structure function relationship)
Number of Ph. D. students completed: 2Number of Ph. D. students at working: 4
Professional Career: Working as Scientist, NCIM Resource Centre, Division of Biochemical Sciences, National Chemical Laboratory,
Pune 411 008 since Feb. 1981
26
Research Experience : (30 years)
Project(s) submitted/being persued/carried out
Title of Project Fundingagency
Duration
1. Microbial Enhanced Oil Recovery2.-Galactosidase from Humicola sp3. Strain improvement for lactic acid production4.Characterization of highly thermostable - Galactosidase from B. stearothermophilus and its application in soymilk and animal feed5.Screening of marine Acinetobacter for production of biosurfactant6.Studies on novel highly acidic Phytase from
Aspergillus niger: Up scaling, partial characterization and its application in poultry feed7. Studies on novel highly acidic Phytase from
Aspergillus niger: Purification, characterization and its application in animal feed and myo-inositol production
DBTDSTCargill, USADST
DBT
DBT
DST
1992-19951995-19981998-20011999-2003
2003-2006
2005-2008
2007-2010
A. Position and HonorsPosition and Employment (Starting with the most recent employment)
Sl No. InstitutionPlace
Position From (Date) To (date)
1 National Chemical Laboratory, Pune Sci.F 1-2-2009 Onwards2 National Chemical Laboratory, Pune Sci.EII 1-3- 2002 31-1-2009
Honors/Awards
Best Poster paper presentation award (1987) at Annual meeting of Society of BiologicalChemists, India held at Trivendrum
Awarded Post doctoral research fellowship from University of Pennsylvania,Philadelphia, USA (Jan 1988 to Jan 1990).
Elected as a Fellow of Maharashtra Academy of Sciences in the year 2010
27
Citations:Paper published in JBC 264: 11699-11705 (1989) has highest citation of 154.
Total Impact Factor : 98.694 (Average IF 2.407) in Nov 2011
Total Citation :621 ; Scripts on Akashwani, Pune for “Nate Nisargashi” rogrammein Marathi - 50
Professional Experience and Training relevant to the Project
1. Experience in maintenanace and preservation of microbial cultures for last 30 years2. Animal feed enzymes: Phytase and alpha-galactosidase.3. Strain improvement and upscaling of solid state and submerged fermentation.4. Structure-function relationship of microbial enzymes5. Lactic acid: Strain improvement and upscaling.
B. Publications (Numbers only)
42 (Research papers, 41; Chapters, 1)Patents :9 (8 Indian and 1 US) and 1 Chapter in Book
Popular articles, 2 &
Chapter in Book:
“Bacterial Biosurfactants and their role in Microbial Enhanced Oil Recovery(MEOR)” in the book “Biosurfactants” edited by Dr. Ramakrishna Sen andpublished by Landes Biosciences/Eureka Publications, George Town, USA (2010)
Patents1. Khire, J. M. and Pant, A. (1995) Process for the production of extra cellular salt
tolerant amylase from Bacillus sp. Indian Patent Application No. 288/DEL/1991
2. Khire, J. M. and Pant, A. (1996) Process for the production of extraacellular salttolerant protease from Bacillus sp. Indian Patent Application No. 287/DEL/1991
3. Khire, J. M., Kotwal S. M. and Khan M. I. (2002) An improved process forpreparation of thermostable α-galactosidase. Indian patent Application No.1193/DEL/1994
4. Khire J. M., Khan M. I. and Shaikh S. A. (1996) A process for the preparation ofthermostable β-galactosidase using thermophilic fungus Mucor sp. Indian PatentApplication No. 504/DEL/1996).
28
5. Mahadik N. D., Gokhale D. V., Bastawde K. B., Khire J. M. and PuntambekarU. S. (2001) An improved process for the preparation of acidic lipase. IndianPatent Application No. NF 49/2001
6. Mahadik, N. D., Gokhale D. V., Bastawde K. B., Khire J. M. and PuntambekarU. S. (2003) Process for the preparation of acidic lipase.
U. S. Patent 6,534,303
7. Khire, Jayant Malhar; Kotwal, Sabirahmed Mehboob; Khan, Mohammed IslamFermentative production of a-galactosidase using a strain of Humicola. IndianIN 186962 A 22 Dec 2001, 10 pp.
8. Soni S. K. and Khire J. M. (2005) A process for preparation of novel acidicphytase (pH 2.5) by Aspergillus niger NCIM 563 under submerged fermentationcondition. Indian Patent CSIR No. NF 180/2005
9. Soni S. K., Depen D., Singh R. P. and Khire J. M. (2008) Novel biocatalyticsynthesis of nanosized hydroxyapetite, myo-inositol and several myoinositolphosphates simultaneously from agricultural residues. CSIR no. NF 0049/2008
Selected peer-reviewed publications (Ten best publications in chronological order)
1. Bhavsar K, Ravi Kumar V and Khire J. M. (2011) High level phytase production byAspergillus niger NCIM 563 in solid state culture: response surface optimization,up-scaling, and its partial chartacterization. J. Ind. Microbiol. Biotechnol. 38: 1407-1417
2. Soni S K, Magdum A & Khire J M (2010) Purification and characterization of twodistinct acidic phytases with broad pH stability from Aspergillus niger NCIM 563.World Journal of Microbiology and Biotechnology 26: 2009-2018 (IF-1.082) C1
2. Joshi D. S, Singhvi M S, Khire J M & Gokhale D V (2010) Strain improvement ofLactobacillus lactis for D-lactic acid production. Biotechnol Lett. 32: 517-520 (IF1.636)
3. Shah P, Bhavsar K, Soni S. K. and Khire J. M. (2009) Strain improvement and up scaling of phytase production by Aspergillus niger NCIM 563 under submerged fermentation condition. J. Ind. Microbiol. Biotechnol. 36, 373-380 (IF 1.798)
4. Dumbrepatil A, Adsul M, Chaudhari S, Khire J and Gokhale D. V. (2008)Utilization of molasses sugar for lactic acid production by Lactobacillusdelbrieckii subsp delbrueckii mutant Uc-3 in batch fermentation. Appl. Environ.Microbiol. 74, 333-335 (IF 4.004)
5. Adsul M., Khire J., Bastawde, K and Gokhale, D. (2007) Production of lactic acidfrom cellobiose and cellotriose by Lactobacillus delbrueckii Mutant Uc-3. Appl.Enviorn. Microbiol. 73, 5055-5057 (IF 4.004)
6. Gote, M. M., Khan M. I. and Khire J. M. (2007) Active site directed chemical
29
modificationof α-Galactosidase from Bacillus stearothermophilus (NCIM 5146): Involvement of lysine, tryptophan and carboxylate residues in the catalytic site. Enzyme Microbial Technology 40, 1312-1320 (IF 1.969)
7. Gote, M., Umalkar, H., Khan, I. and Khire, J. M. (2004) Thermostable - galactosidase from Bacillus stearothermophilus (NCIM 5146) and its application in the removal of flatulence causing factors from soymilk. Process Biochem. 39, 1723-1729 (IF 2.336)
8.. Mandviwala, T. and Khire, J. M. (2000) Production of high activity thermostable phytase from thermotolerant Aspergillus nigerin solid state fermentation. J. Industrial Microbiol. and Biotechnol. 24, 237-243. (IF 1.681)9.. Kotwal, S. M. , Gote, M. M., Sainkar, S. R., Khan M. I. and Khire, J. M. (1998) Production of -galactosidase by thermophilic fungus Humicola sp in solid-state
fermentation and its application in soymilk hydrolysis. Process Biochemistry 33,337-343. (IF 2.336)
10. Bishayee, S., Majumdar, S., Khire, J. M. and Das, M. (1989) Ligand-induceddimerization of platelet-derived growth factor receptor. J. Biol. Chem. 264, 11699-11705 (IF 5.581)
30
Name : .Dr.Mukund V.Deshpande.
Designation :Scientist G
Department/Institute/University : …Biochemical Sciences
Date of Birth : 12-9-1952….... Sex (M/F) …M SC/ST : ..NA..
Education (Post-Graduation onwards & Professional Career)
Sl No. InstitutionPlace
DegreeAwarded
Year Field of Study
1 University of Pune M.Sc. 1975 Microbiology2 University of Pune Ph D 1982 Biochemistry*3 University of Pune D Sc 1994 Microbiology**
*Title: Studies on Cellulases and Hemicellulases; **Title: Fungal Differentiation andBiochemical Correlates.
C. Position and HonorsPosition and Employment (Starting with the most recent employment)
Sl No. InstitutionPlace
Position From (Date) To (date)
1 National Chemical Laboratory, Pune Sci.G 1-3-2009 Onwards2 National Chemical Laboratory, Pune Sci.F 1-3- 2004 28-2-20093 National Chemical Laboratory, Pune Sci.EII 1-3- 1998 28-2-2004
Honors/Awards
Doctor of Science degree has been awarded by the University of Pune, 1994. Elected as Fellow of Maharashtra (State) Academy of Sciences in November 1994.
Awarded Department of Biotechnology Overseas (Short-term) Associateship (1995). Awarded Commonwealth Science Council Fellowship (1998)
Citations:The research papers published in Anal. Biochem. (138:481-, 1984) is one of the
highly cited (98 citations) papers published from NCL (SMIS, October 8, 2001).
The research paper published in Naturwissenschaften 87: 236-240 (2000) featuredin Newsindia, a Nature's science & policy round-up for India, September 2000
The research paper published in J. Invertebr. Pathol. 85:80-88 (2004) featured inNewsindia, a Nature's science & policy round-up for India, June 2004
Professional Experience and Training relevant to the Project
1. Fungal morphogenesis: Dimorphism in Benjaminiella poitrasii and Yarrowia
31
lipolytica; Conidiation in Conidiobolus coronatus2. Carbohydrate based enzymes: Cellulases, chitinases and amylolytic enzymes.4. Other enzymes: Proteinases, nucleases, glucose isomerase and polyphenol oxidase.
1. Chitin metabolism as a target for biocontrol agents/ antifungal drugs2. Biosensors.
D. Publications (Numbers only)
111 (Research papers, 68; Reviews, 12; Chapters, 31)Popular articles, 20 (English, Hindi and Marathi); Report, 3; Books, 5; Patents, 5;Technical brochures, 2
Total IF 120.447 (Ave.1.52) Total citations: 975 (as on July 2011) (Ave. citations12.34)
Patents
1.A method for preparation of mycolytic enzymes containing mainly chitinase.(M.Deshpande, M. Desai, M.Chitnis, and R.Patil). Indian patent filed (March 2001).
2.A process for the preparation of mycolytic enzymes containing mainly chitosanase(M.Deshpande, R.Patil, M.Chitnis, and R.Seeta Laxman). Indian patent 193300 datedJuly 17, 2004
3.Bile acid derived steroidal dimers with novel amphiphilic topology having antifungalactivity (Salunke D.B., Hazra B.G., Pore V.S., Bhat M.K., Nahar P.B. and DeshpandeM.V.). US patent 7,235,678, June 26, 2007
4. New substituted 1,4-dioxa-8-azaspiro(4,5)decane compounds used as fungicide forwide range of fungi such as Pyricularia oryazae, Rhizoctonia solani, Pythiumaphanidematum, Dreshlera oryazae, Collatrotricum capsicum, and Fusarium oxysporum(Deshpande M V, Deshpande S R, Shirazi F, Chaudhary P M, Rao N M, Mohanty B,Sharma N N, Bachhawat A K, Kaliannan G, Paul S, Kumar R, Rao B V, Gawali B B,Reddy V V N, Yadav J S, Nelavelli M R, Raj K, Bommena V R, Vaddu V N R) PatentNumber(s).:WO2010109299-A2; WO2010109299-A3; IN200900612-I1
4. A composition for controlling woolly aphid infestation and a method of treatment(M.Deshpande, M.Kulkarni and S. Chavan) Indian patent filed (January 2004)
Selected peer-reviewed publications (Ten best publications in chronological order)
Ghormade V., Deshpande M.V. and Paknikar K.M (2011) Perspectives for nano-biotechnology in plant protection and nutrition. Biotechnol. Adv. 29: 792–803 (IF 8.25)
32
Yadav P and Deshpande M.V. (2010) Fungus- fungus and fungus–insect interactions.Biopestic. Int. 6:21-35
Chaudhary P.M., Chavan S.R., Shirazi F., Razdan M., Nimkar P., Maybhate S.P., LikhiteA.P., Gonnade R., Hazara B.G., Deshpande M.V., and Deshpande S.R. (2009)Exploration of click reaction for the synthesis of modified nucleosides as chitin synthaseinhibitors. Bioorg. Med. Chem. 17: 2433-2440 (IF 2.662)
Kulkarni S.A., Ghormade V., Kulkarni G., Kapoor M, Chavan S.B., Rajendran A., PatilS.K., Shouche Y., and Deshpande M.V. (2008)Comparison of Metarhizium isolates forbiocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae) in chickpea. BiocontrolSci. Technol. 18: 809-828. (IF 1.087)
Nahar P.B., Kulkarni S.A., Kulye M.S., Chavan S.B.,Kulkarni G., Rajendran A.,YadavP.D., Shouche Y. and Deshpande M.V. (2008) Effect of repeated in vitro sub-culturingon the virulence of Metarhizium anisopliae against Helicoverpa armigera (Lepidoptera:Noctuidae). Biocontrol Sci. Technol.18: 337-355 .
Gholap A.R., Toti K.S., Shirazi F., Ratna Kumari, Bhat M.K., Deshpande M.V. andSrinivasan K.V. (2007) Synthesis and evaluation of antifungal properties of a series ofthe novel 2-amino-5-oxo-4-phenyl-5, 6, 7, 8-tetrahydroquinoline-3-carbonitrile and itsanalogues. Bioorg. Medicinal Chem. 15:6705-6715
Ghormade V., Shastry P., Chiplunkar J. and Deshpande M.V. (2005) Determination ofploidy of a dimorphic zygomycete Benjaminiella poitrasii and the occurrence of meioticdivision during zygospore germination. J. Agric. Technol. 1:97-112.
Amin A., Joshi M. and Deshpande M.V. (2004) Morphology-associated expression ofNADP-dependent glutamate dehydrogenases during yeast-mycelium transition of adimorphic fungus Benjaminiella poitrasii. Antonie van Leeuwenhoek 85: 327-334
Nahar P.B., Ghormade V. and Deshpande M.V. (2004) The extracellular constitutiveproduction of chitin deacetylase in Metarhizium ansiopliae: Possible edge to entomo-pathogenic fungi in the bio-control of insect pest. J. Invertebr. Pathol. 85: 80-88(Featured in Newsindia, a Nature's science & policy round-up for India, June 2004).
Chitnis M., Munro C.A., Brown A.J.P., Gooday G.W., Gow N.A.R. and Deshpande M.V.(2002) The zygomycetous fungus, Benjaminiella poitrasii contains a large family ofdifferentially regulated chitin synthase genes. Fungal Genetics Biol. 36:215-223
Chitnis M. and Deshpande M.V. (2002) Isolation and regeneration of protoplastsfrom the yeast and mycelial form of a dimorphic fungus Benjaminiella poitrasii : Roleof chitin metabolism for morphogenesis during regeneration. Microbiol Res. 157: 29-37.
Patil R.S., Deshpande A.M., Natu A.A, Nahar P., Chitnis M., Ghormade V.,
33
Laxman R.S., Rokade S. and Deshpande M.V. (2001) Biocontrol of root infectingplant pathogenic fungus, Sclerotium rolfsii using mycolytic enzymes and chitinmetabolism inhibitors singly and in combination. J. Biol. Control . 15:157-164
Ghormade V. and Deshpande M.V. (2000) Fungal spore germinationinto yeast or mycelium : Possible implications of dimorphism in evolution and humanpathogenesis. Naturwissenschaften 87: 236-240. (Featured in Newsindia, a Nature'sscience & policy round-up for India, September 2000).
List maximum of five recent publications relevant to the proposed area of work.
Ghormade V., Deshpande M.V. and Paknikar K.M (2011) Perspectives for nano-biotechnology in plant protection and nutrition. Biotechnol. Adv. 29: 792–803 (IF 8.25)
Yadav P and Deshpande M.V. (2010) Fungus- fungus and fungus–insect interactions.Biopestic. Int. 6:21-35
Chaudhary P.M., Chavan S.R., Shirazi F., Razdan M., Nimkar P., Maybhate S.P., LikhiteA.P., Gonnade R., Hazara B.G., Deshpande M.V., and Deshpande S.R. (2009)Exploration of click reaction for the synthesis of modified nucleosides as chitin synthaseinhibitors. Bioorg. Med. Chem. 17: 2433-2440 (IF 2.662)
Kulkarni S.A., Ghormade V., Kulkarni G., Kapoor M, Chavan S.B., Rajendran A., PatilS.K., Shouche Y., and Deshpande M.V. (2008)Comparison of Metarhizium isolates forbiocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae) in chickpea. BiocontrolSci. Technol. 18: 809-828. (IF 1.087)
Nahar P.B., Kulkarni S.A., Kulye M.S., Chavan S.B.,Kulkarni G., Rajendran A.,YadavP.D., Shouche Y. and Deshpande M.V. (2008) Effect of repeated in vitro sub-culturingon the virulence of Metarhizium anisopliae against Helicoverpa armigera (Lepidoptera:Noctuidae). Biocontrol Sci. Technol.18: 337-355 .
Patil R.S., Ghormade V. and Deshpande M.V. (2000) Chitinolytic enzymes: Anexploration. Enzyme Microbial Technol. 26 473-483.
C. Research SupportOngoing Research Projects
1.Development of sustainable pest management strategies basedon entomopathogenicfungi and cuticle degrading enzyme complex against mealy bug, Maconellicoccushirsutus (Green) on grapes in India (April2009- March2012, DBT,New Delhi)2.Development of environmentally benign nanomaterial-based enzyme formulations forbiocontrol of plant pathogens and pests (Nov.2009- Oct. 2012, DBT, New Delhi)3.Transferring mycoinsecticide technology to the private sector (Nov.2009- March 2011,Indo-Swiss Collaborative programme of DBT, New Delhi and SDC, Berne, Switzerland;SBIRI project approved)
34
Completed projects
Sl No. Title of Project Funding Agency Amount Date of sanctionand Duration
1 Development of amycoinsecticide againstHelicoverpa armigera inpluses: from the laboratory tothe market
SDC and DBT Rs.38,00,000/-
September20043 years
2 Polyamines, dimorphism inYarrowia lipolytica and thepetroleum oil degradation inmarine environment
DST Rs.9,13,500/-
July20053 years
3 Development of novelfungicides
CSIR Rs.50,72,000/-
October 2004Extended up toMarch 2008
4 Scale-up, field demonstrationand techno-economics ofenzyme complex fromMyrothecium verrucaria as abiocontrol agent
DBT Rs.18,10,000/-
June 20034 years
5 The Biochemical andmolecular profile of the winespoilage yeasts and filamentousfungi
DBT Rs.18,17,000/-
April-20072 years
Completed Research Projects (State only major projects of last 3 years)Sl No. Title of Project Funding Agency Amount Date of
completion1 Development of novel
biopesticides based on fungi/bacteria exhibiting dualantagonistic activity againstplant pathogenic fungal andinsect pests
DBT Rs.15,23,000/-
(April 2002-March 2005;Indo-BelarusCollaborationinBiotechnology,DBT, NewDelhi)
2 Microbial control ofpests:Entomopathogenicfungi asmycoinsecticides"(April2000- March 2004; Indo-Swiss Collaborative
DBT/SDC Rs.68,00,000/-
(2000-2004)
35
programme)3 NAD- and NADP-dependent
glutamate dehydrogenasesfrom a dimorphic fungus,Benjaminiella poitrasii:Purification andcharacterization (May 1999-April 2003; DST, New Delhi)
DST Rs.15,00,000/- (2000-2004)
4 Development of biocontrolagents for the control of plantpathogenic fungi and insectsusing chitin as a target(August 1997- July 2000;DBT, New Delhi)
DBT (1997-2001)
5 Exploration & exploitation ofmicrobial wealth of India fornovel compounds andbiotransformation processes(c) Chitosan production byfermentation using the fungusBenjaminiella poitrasii &applications in chitosan-based drug carriers andhuman health care items
CSIR Rs. 4,90,850/- April20034 years
Place :Date : Signature of Investigator
