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Sony Biotechnology Inc. Data Sheet, De Novo FCS Express Software 6.0 Page 1 <1.00 0 2 4 6 8 10 12 14 16 18 20 22 24 3.00 >259064.00 25 24 23 22 21 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0 CD3 : BV785 - Area (# of Events) <11.94 98.11 >184.29 tSNE Y tSNE X -26.02 -13.99 -1.95 10.09 22.13 26.95 12.96 -1.04 -15.04 -29.04 Intensity Height (Wavelength) Grans 420.0 515.0 610.0 705.0 800.0 10 5 10 4 10 3 10 2 Monos Lymphs 10 5 10 6 FOXP3 : PE-Dazzle594 - Area CD4 : BV421 - Area 10 4 10 3 -10 2 10 2 -10 3 10 3 10 4 10 5 10 6 Intensity Height (Wavelength) FOXP3 + FOXP3- 420.0 515.0 610.0 705.0 800.0 10 7 10 6 10 5 10 4 10 3 10 2 10 1 Intensity Height (Wavelength) FOXP3 + FOXP3- 420.0 515.0 610.0 705.0 800.0 10 7 10 6 10 5 10 4 10 3 10 2 10 1 10 5 FOXP3 : PE-Dazzle594 - Area CD4 : BV421 - Area 10 4 10 3 10 2 10 2 -10 2 10 3 10 4 10 5 10 6 SSC - Area (x 1000) CD45 : BV711 - Area Lymphs 262.1 196.6 131 65.5 10 2 -0.1 -10 3 10 3 10 4 10 5 10 6 Intensity Height (Wavelength) FOXP3 + FOXP3- 420.0 515.0 610.0 705.0 800.0 10 7 10 6 10 5 10 4 10 3 10 2 10 1 De Novo FCS Express Software 6.0 for SA3800 and SP6800 Spectral Analyzers Spectral Analyzers from Sony now include FCS Express, version 6 from De Novo Software. FCS Express version 6 supports direct loading of Sony Spectral data files to provide customers with new capabilities and analysis tools. Native support for Sony spectral data files ensures tight integration with Sony software for sophisticated data transformations and visualizations. Expanded capabilities now available to Sony users also include for batch processing, custom calculations and file export into a variety of popular formats. Real Time Feedback FCS Express 6.0 lets users create dot and spectral plots with live gating. Live gating allows users to find positive and negative populations quickly and easily for added assurance about your target populations. FCS Express Software 6.0 supports the SA3800 and the SP6800 cell analyzers. Expanded Data Visualization Native support for Sony spectral data files in FCS Express 6.0 allows for tight integration with De Novo data visualization features. Spectral, FCS and CSV files are directly read by the software to enable more in-depth visualization of your data. FCS Express 6.0 supports a range of visualizations including spectral overlays, tSNE, Spade, and heat maps. These data visualizations can be used to identify a number of challenges with flow data allowing you to cluster or isolate rare populations quickly and confidently.

De Novo FCS Express Software 6.0 for SA3800 and SP6800 …€¦ · Data Sheet, De Novo FCS Express Software 6.0 Page 1 259064.00

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Page 1: De Novo FCS Express Software 6.0 for SA3800 and SP6800 …€¦ · Data Sheet, De Novo FCS Express Software 6.0 Page 1 259064.00

Sony Biotechnology Inc.

Data Sheet, De Novo FCS Express Software 6.0 Page 1

<1.00

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4

6

8

10

12

14

16

18

20

22

24

3.00 >259064.00

25 24 23 22 21 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 0

CD

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of

Even

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SPADE Cluster ID

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-29.04

Inte

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Height (Wavelength)Grans

420.0 515.0 610.0 705.0 800.0

105

104

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Monos Lymphs

105

106

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102

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103 104 105 106

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Height (Wavelength)

FOXP3 +

FOXP3-

420.0 515.0 610.0 705.0 800.0

107

106

105

104

103

102

101

Inte

nsi

ty

Height (Wavelength)

FOXP3 +

FOXP3-

420.0 515.0 610.0 705.0 800.0

107

106

105

104

103

102

101

105

FOX

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104

103

102

102

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103 104 105 106

SSC

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(x 1

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CD45 : BV711 - Area

Lymphs

262.1

196.6

131

65.5

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-103 103 104 105 106

Inte

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Height (Wavelength)

FOXP3 +

FOXP3-

420.0 515.0 610.0 705.0 800.0

107

106

105

104

103

102

101

De Novo FCS Express Software 6.0 for SA3800 and SP6800 Spectral Analyzers

Spectral Analyzers from Sony now include FCS Express, version 6 from De Novo Software. FCS Express version 6 supports direct loading of Sony Spectral data files to provide customers with new capabilities and analysis tools.

Native support for Sony spectral data files ensures tight integration with Sony software for sophisticated data transformations and visualizations. Expanded capabilities now available to Sony users also include for batch processing, custom calculations and file export into a variety of popular formats.

Real Time FeedbackFCS Express 6.0 lets users create dot and spectral plots with live gating.

Live gating allows users to find positive and negative populations quickly and easily for added assurance about your target populations.

FCS Express Software 6.0 supports the SA3800 and the SP6800 cell analyzers.

Expanded Data VisualizationNative support for Sony spectral data files in FCS Express 6.0 allows for tight integration with De Novo data visualization features. Spectral, FCS and CSV files are directly read by the software to enable more in-depth visualization of your data.

FCS Express 6.0 supports a range of visualizations including spectral overlays, tSNE, Spade, and heat maps. These data visualizations can be used to identify a number of challenges with flow data allowing you to cluster or isolate rare populations quickly and confidently.

Page 2: De Novo FCS Express Software 6.0 for SA3800 and SP6800 …€¦ · Data Sheet, De Novo FCS Express Software 6.0 Page 1 259064.00

Sony Biotechnology Inc.

Data Sheet, De Novo FCS Express Software 6.0 Page 2

Plate and High Content AnalysisWith FCS Express 6 you can import plate or high content screening data and transform that data to a variety of analytical formats. This includes heat maps, overlays with radius visualization, and parameter statistics.

Custom CalculationsQuickly and easily perform data analysis with integrated spreadsheets, custom calculations, charting and regression analysis. FCS Express 6.0 gives you ultimate flexibility to understand and interpret your data.

Report and BatchFCS Express 6.0 lets you batch process any number of samples with easy and fast only one-click . To support pre-sentation of your data FCS Express 6.0 also lets you export your findings directly to directly to PowerPoint, PDF, and Excel in high quality.

©2017 Sony Biotechnology Inc. All rights reserved. Sony and the Sony logo are trademarks of Sony Corporation. De Novo Software and FCS Express are trademarks of De Novo Software. For non-clinical research use only. Not for use in diagnostic or therapeutic procedures, or for any other clinical purpose The SA3800 and SP6800 spectral analyzers are class 1 laser products. 12.04.050217

FCS Express Professional 6.0 is a product of De Novo Software, licensed to Sony Biotechnology