��•  P2 •  P8 •  CsCl Preparation of Plasmid DNA P14� Biacore T200 14F P18 �

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BioMed Resource Core

1.  R1438 & R1439

2.  9:00~17:00

3.  ( ) 4. A. B. C. D.

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(1) (2) …(3) CFP GFP YFP RFP…(4) (5) siRNA miRNA(6) Adenoviral lentiviral retroviral vectors (7)

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74 E.coli competent cell Staphylococcus Streptococcus

Yeast two-hybrid cDNA library 9

310

(gene construction)

stable clone

Yeast two hybrid assay

cDNA library

primary culture

cDNA library Genomic DNA library

mini- /midi- kit CsCl banding

BL21 (DE3) Baculovirus

1. 2. 3. 4.

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Yeast Core Facility for Human Disease Model��

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C. elegans Core Facility for Human Disease Model

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• Drosophila Core Facility for Human Disease Model

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Mouse Core Facility for Human Disease Model �

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(e-mail: ltjang@ntu.edu.tw)

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•  •  strain 2000 •  500

•  NT$ 600/strain

•  RNAi knock down ( ) NT$ 800/clone or gene

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DNA(plasmid) DNA DNA

DNA DNA

DNA DNA

1998 DNA

”CsCl banding”

CsCl banding DNA

supercoiled form DNA

(transfection)

�  CsCl gradient & DNA banding :

CsCl (

1.91g/ml) Cs

Cs

Cs gradient

DNA CsCl

78000 rpm DNA

CsCl DNA

1.7g/ml 1.6g/ml RNA

1.8g/ml banding DNA

CsCl ultracentrifuge DNA

�  Ethidium Bromide & DNA banding

DNA DNA DNA

EtBr

AT/ GC

EtBr

(unwind) DNA

supercoiled form DNA linear form EtBr

EtBr

CsCl DNA

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