Biacore Insight Evaluation Software · 2018-11-06 · gelifesciences.com Data file, KA1995180518DF Label-free interaction analysis Biacore™ Insight Evaluation Software Biacore™

Data file, KA1995180518DFgelifesciences.com

Label-free interaction analysis

Biacore™ Insight Evaluation SoftwareBiacore™ Insight Evaluation Software offers intuitive workflow and application-specific evaluation software packages, tailored for Biacore systems (Fig 1). The modular design of the software packages allows a more cost-efficient configuration to evaluate screening and characterization data from surface plasmon resonance (SPR) systems including Biacore 8K, Biacore 8K+, Biacore T200, and Biacore S200.

Biacore Insight Evaluation Software offers:

• Efficient method definition, operation, and evaluation: rapid data overview and analysis combined with easy data handling shortens time to decision.

• Cost-efficient software: pay for what you need starting with basic functionality that expands to more advanced extensions as your research evolves.

• Convenient license control: floating e-licenses enable software access between users and computers.

• Multisystem support and easy data handling: one data analysis solution for Biacore T200, Biacore S200, Biacore 8K, and Biacore 8K+ systems reduces training requirements.

• Easily export and share results: transfer large data sets or editable figures with ease and benefit from a presentation generator to rapidly share your results.

The basic screening and characterization functionality of Biacore Insight Evaluation Software ensures faster, data-driven decisions in SPR screening and characterization. The Extended Screening and Characterization package is available as an add-on extension package, with dedicated software tools for screening of LMW fragments. The extension package speeds up assay setup and streamlines evaluation, providing greater flexibility and tools to analyze complex biomolecular interactions.

Concentration functionality is available as the Concentration and Potency Extension package with dedicated software tools for concentration analysis, parallel line analysis (PLA), and EC50 evaluation. This add-on allows for streamlined

evaluation of concentration determinations and enables potency determinations.

Biacore Insight Evaluation Software can be equipped will full support for working in regulated environment through the GxP extension package. This add-on provides validated software supporting GLP/GCP/GMP and 21 CFR Part 11 compliance.

Basic screening and characterization functionalityBiacore Insight Evaluation Software offers a range of powerful tools for confident and reliable interaction analysis suitable for users of all levels of experience. The Basic Screening and Characterization package is the foundation of Biacore Insight Evaluation Software and supports many applications with the most commonly used tools. Functionality of the software may be further enhanced by a growing number of extensions.

Fig 1. Biacore Insight Evaluation Software is a modular design software for evaluation of interaction analysis data, screening, kinetics, affinity, concentration and relative potency data.

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Efficient evaluation of affinity and kinetic dataBiacore Insight Evaluation Software enables kinetic and affinity evaluation with a few simple clicks. The software is equally suited for the rapid analysis of large screening campaigns as well as deep kinetic characterization of a single interaction. Generic tools scale with the size of your experiment rendering fast results you can trust, irrespective of the number of samples analyzed.

The flexible evaluation interface (Fig 2) is configurable to maximize the space for your most important tasks at any time:

• Get a rapid overview and qualify your data fast

• Utilize flexible tools for customized data analysis

• Easily export and share results

Fig 2. The flat interface of Biacore Insight Evaluation Software provides full overview while offering flexible tools for customized data analysis. The overview allows convenient simultaneous visualization of kinetics and affinity data providing Result table, On-off rate chart, and KD chart.

Fig 3. The result export feature allows easy export of selected results to Microsoft Excel format for further evaluation.

Simultaneous visualization of results from thousands of samples

Thousands of samples from multiple runs can be visualized simultaneously and the results presented in a Result plot. The Result plot provides efficient, flexible, easy-to-use tools to process the data of interest. Absolute or referenced data can be plotted and double-referenced by applying Blank subtraction. Sample responses can easily be harmonized with respect to the molecular weight of the analyte or the level of captured ligand respectively, making the entire data set comparable.

Adjustment for controls normalizes sample responses to the selected positive (and optional negative) controls, to correct for drift in surface activity during the course of the assay. Cutoff/Ranking is manually set. Alternatively, control-based automatic cutoff allows a cutoff to be set at the selected number of standard deviations in relation to the control.

Evaluation methods to maximize speed

The data evaluation process may be standardized and speed up by applying predefined evaluation methods, shortening time to decision. The software comes with application-specific Evaluation methods that may be applied as is or used as template for optimized user-defined evaluation methods. Relevant evaluation parameters may be saved in Evaluation methods, avoiding repetitive tasks. When opening a run file with the selected Evaluation method, the first results are obtained in minutes.

Fast export of selected or comprehensive data

The flexible result export feature in Biacore Insight Evaluation Software provides the means to export selected or comprehensive data for continued data processing, result reporting, or storage in the company database. Data is exported in a Microsoft® Excel® format. Evaluation items included in the export may easily be selected and detailed settings for each item defined. Sensorgrams or plots may be included in the export as raster images in three different sizes. Images may also be exported as vector graphics. Result tables are exported either using a comprehensive format or using the same format as defined in the evaluation data. Results are therefore always presented in a preferred format to facilitate reporting or adoption into other software.

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Fig 4. (A) Presentation preparation in Biacore 8K Evaluation Software (above) vs the new way of working using the built-in Presentation generator (below). (B) Presentation generator promotes a quick and easy way to share data in a presentable format.

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(B)

Data export

Presentation export

Chart formatting Layout formattingChart creation Chart import Present Biacore data

Present Biacore data

Evaluation

Evaluation

Biacore Insight Evaluation Software

Biacore Insight Evaluation Software

Microsoft Excel Microsoft PowerPoint

Microsoft PowerPoint

Biacore 8K Evaluation SoftwareCreate presentation using data export

Biacore Insight Evaluation SoftwareReady-to-use slides

Presentation preparation is just “a click away”

Data may be easily transferred into Microsoft PowerPoint® format (Fig 4). Simply switch to the Presentation generator to select evaluation items and export them as “native” PowerPoint charts and tables.

Data may be further modified using PowerPoint extensive tool set and layouts. This new way of working with Biacore data sets will make it even easier to share data with your colleagues and peers.

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Binding level screen

Clean screenNew library

Affinity screen

Validation

Structural Medicinal chemistry

Competition screen

Fig 6. Selecting a kinetic fit model in the Extended Screening and Characterization package.

Fig 7. Typical analysis workflow for FBDD and small-molecule screening using Biacore systems. The workflow generates reliable and conclusive results prior to structure analyses and transfer to medicinal chemistry.

Fig 8. LMW fragment library cleanup using Clean screen evaluation. Sticky, residual binding is evaluated by assessing the difference in absolute baseline response between adjacent cycles in a run.

Fig 5. Unlocking the Extended Screening and Characterization package, which provides advanced features for LMW fragment screening beyond those available from the basic software package.

Bring greater flexibility to the software with extensionsBuilding on the framework of the Basic Screening and Characterization package, the Extended Screening and Characterization package may be selected upon startup and unlocked for more advanced features or dedicated application support for screening of LMW fragments to speed up assay setup and streamline evaluation (Fig 5).

With a growing number of extension packages, you will be able to use Biacore Insight Evaluation Software as your research expands and evolves.

Efficiently analyze challenging interactions

• Characterize a wider range of compound-target interactions using additional kinetic fitting models and evaluation settings.

• Evaluate challenging interactions such as LMW fragment binding to transmembrane G protein-coupled receptors (GPCRs) and bivalent protein interactions with relative ease.

• Dedicated software tools for screening of LMW fragments to speed up assay setup and streamline evaluation.

The Extended Screening and Characterization package enables a wider variety of fitting models (Fig 6) using maximum response (Rmax) control and Blank subtraction, and gives access to more customization options (grouping of data channel vs channel, color by curve markers). The extension package also has three kinetic and/or evaluation templates—Clean screen, Binding level screen, and Affinity screen. These evaluation methods guide the user and shorten time to results for the entire SPR workflow in fragment-based drug discovery, FBDD (Fig 7).

Clean screen (Fig 8) enables efficient LMW fragment library cleanup by identifying sticky, residual binding to the sensor chip surface that masks subsequent cycles. Sticky, residual binding is evaluated by assessing the difference in absolute baseline response between adjacent cycles in a run.

Binding level screen (Fig 9) identifies primary hits to the target to find and exclude fragments with atypical binding behavior. Fragment hits at this stage are identified by both binding response and by curve shape, which gives much deeper insight into the binding characteristics of the fragments.

Affinity screen (Fig 10 and 11) verifies target binding and enables an estimate of steady-state affinities of fragments selected in previous screens. Affinities for fragments are often in the millimolar to high micromolar range. Figure 11 shows Affinity screen sensorgram evaluations.

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Fig 9. Binding level screen performed by assessing reports point values and binding behavior based on curve shapes after reference subtraction and solvent correction. Fragments often bind very rapidly to the primary site followed by binding to secondary and tertiary sites. By using report point Binding_early placed early during the fragment injection, the impact of such secondary and tertiary binding is reduced. An ideal fragment is expected to bind rapidly to steady state followed by rapid dissociation.

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(B)

Fig 10. With Affinity screen, affinity determinations are evaluated by plotting the response at equilibrium of a concentration series (A) and then fitting the data to a steady-state affinity model (B). Affinity is normally estimated by fitting the data to a one-site model, but selected data can easily be fitted with a multisite model where appropriate (B).

Fig 11. Evaluation of Affinity screen sensorgrams.

While providing guidance, the templates are fully flexible, may be easily adjusted to fit specific assay needs, and may be saved upon customization to speed up future analyses.

Confident concentration and potency analysis

• Determine active protein concentration with confidence, short time to results with automation and minimum hands-on time.

• Increase assay precision and reproducibility over a wide dynamic range.

• Reliably determine drug product amount and activity using surrogate potency assays, reducing costs and saving time

• Eliminate errors in data transfer with built-in Parallel Line Analysis tool

The Concentration and Potency Extension package enables measurement of the biologically active concentration, not only the total protein amount that would be obtained from an A280 determination. The precision and automation of the system reduces hands-on time and generates highly reproducible data over a wide dynamic range with CV typically below 5%. The software supports both parallel and serial run modes allowing you to select the optimal mode based on your needs.

Calibration curves may be generated using four-parameter or linear-fitting models. The software supports evaluation of the samples using single and average calibration curves. For long runs in serial mode, interpolation of calibration curves to compensate for drifts in the assay may be performed to generate highly reliable data. The possibility to include control samples allows you to ensure a rigorous quality control of your assay.

Drug product amount and activity are reliably determined using surrogate potency assays, reducing costs and saving time. Potency assays can be easily evaluated using the built-in software tools of the Concentration and Potency Extension package. This allow you to determine the potency of your sample relative to a reference sample. This streamlines the analysis workflow and lowers the risk for user mistakes and data manipulation when transferring data between different software. Potency analyses are performed using Parallel Line Analysis (PLA) or half-maximal response (EC50) determinations. The PLA functionality makes the assessment of potency based on a linear fit to the linear part of the response vs logarithmic concentration assuming a common slope (Fig 12). The EC50 determination is based on a four-parameter equation being fitted to the response vs concentration.

Fig 12. Evaluation of potency data using tool for parallel line analysis.

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Multisystem support and easy data handlingBiacore Insight Evaluation Software analyzes data derived from Biacore 8K+, Biacore 8K, Biacore T200, and Biacore S200 systems for the supported applications (Table 1). Users analyze data from different sources using the same familiar interfaces, features, workflows, and graphical presentation of data. This reduces training needs if multiple and different Biacore instruments are used.

A detailed list of basic features as well as the content of the advanced Extended Screening and Characterization extension are listed in Table 2.

Table 1. Biacore Insight Evaluation Software compatibility for different Biacore instrument models

Extension package

Biacore 8K Biacore 8K+

Biacore T200 Biacore S200

Basic Screening and Characterization

Extended Screening and Characterization

Concentration and Potency

GxP

Table 2. Features of the available screening and characterization packages available for Biacore Insight Evaluation Software

Feature Basic Screening and Characterization Extended Screening and Characterization

Fitting models • 1:1 binding• Steady-state affinity

• 1:1 dissociation• Bivalent analyte• Heterogenous ligand• Two-state reaction

• Steady-state affinity (constant Rmax)

• Steady-state affinity (constant Rmax and multisite)

Max. response (Rmax) – • Purpose Rmax control • Adjust Rmax for ligand decay

Grouping • Channel• Analyte solution• Analyte concentration

• Cycle• Analysis step purpose• Analysis step name

• Channel vs Channel

Adjustments • Molecular weight adjustment

• Capture/ligand adjustment• Adjustment for controls

• Blank subtraction

Sensorgrams • Sensorgram subtraction • Sensorgram normalization

Evaluation methods • LMW screen• Antibody/general screen • LMW kinetics and affinity

• Antibody/general kinetics and affinity

• Clean screen• Binding level screen

• Affinity screen

Color by • Cycle• Channel• Sensorgram ID

• Immobilized ligand level• Analyte solution• Analyte concentration

• Curve markers

Work in GxP-regulated environments - optional software for GxP compliance

An optional GxP Extension package allows Biacore 8K and Biacore 8K+ to integrate seamlessly into GxP-regulated workflows. The package provides validated software supporting GLP/GCP/GMP and 21 CFR Part 11 compliance, and includes validation support. For full validation support of the system, the package can be supplemented with GE Qualification Service.

Features in the GxP Extension package include:

• Data integrity – access control and enforced version handling

• User authorization levels – administrator, developer and user levels set access rights to software functions

• Procedures for operational control – enables assay run and evaluation settings to be locked together in routine assays

• Audit trail – tracks record modifications and maintains complete version histories for published procedures

The software has been developed in accordance with an accepted development model to ensure adequate validation.

SQL

SQL

SQL

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Fig 13. (A) Software installed on different computers connecting to a shared network database and/or network e-licensing server. (B) Software installed on system controller (computer connected to Biacore 8K) and additonal computers that all connect to a shared network database and/or network e-licensing server. (C) Installation on local database and e-licensing server on a standalone/portable computer.

(A)

(B)

(C)

e-LicensingBiacore Insight Evaluation Software introduces floating licenses that allow sharing of access rights between users and computers and removes restrictions on the number of simultaneously installed copies of the software. Users are able to electronically secure a machine license for one computer, or a concurrent license for networks. In addition, time-limited licenses are available, allowing you to scale the number of licenses needed for more flexibility.

Networking capabilitiesBiacore Insight Evaluation Software may be deployed in several different ways to take advantage of the functionality available in a networked configuration (Fig 13).

Advantages of network setup include:

• Data sharing

• Floating licenses for optimized usage

• Centralized administration

Technical specificationsOperating system: Windows® 7 (Professional with SP1, 64-bit) or Windows 10 (64-bit).

Database: Biacore Insight Evaluation Software includes SQL Server Express 2014 Release 2. Performance improvements are seen with SQL Server Standard, SQL Server Enterprise, or SQL Data Warehouse (available separately from Microsoft).

Contact your local GE representative for full technical specifications.

Ordering informationProduct Product code

Biacore Insight Evaluation Software, Permanent Single License 29310602

Biacore Insight Evaluation Software, Permanent 5 License Pack 29310603



Biacore Insight Evaluation Software, 1 yr Single License 29310606

Biacore Insight Evaluation Software, 1 yr 5 License Pack 29310607



Screening and Characterization extension packages

Biacore Insight Evaluation Software Screening and Characterization Extension, Permanent Single License 29310610

Biacore Insight Evaluation Software Screening and Characterization Extension, Permanent 5 License Pack 29310611



Biacore Insight Evaluation Software Screening and Characterization Extension, 1 yr Single License 29310614

Biacore Insight Evaluation Software Screening and Characterization Extension, 1 yr 5 License Pack 29310615



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KA1995180518DF

Concentration and Potency extension packages Product code

Biacore Insight Evaluation Software, Concentration and Potency Extension, Permanent Single License 29332204

Biacore Insight Evaluation Software, Concentration and Potency Extension, Permanent 5 License Pack 29332205



Biacore Insight Evaluation Software, Concentration and Potency Extension, 1 yr Single License 29332208

Biacore Insight Evaluation Software, Concentration and Potency Extension, 1 yr 5 License Pack 29332209



GxP extension packages Product code

Biacore Insight Evaluation Software, GxP Extension, Permanent Single License 29332212

Biacore Insight Evaluation Software, GxP Extension, Permanent 5 License Pack 29332213



Biacore Insight Evaluation Software, GxP Extension, 1 yr Single License 29332216

Biacore Insight Evaluation Software, GxP Extension, 1 yr 5 License Pack 29332217



Biacore Insight Evaluation Software, GxP Extension, Trial License 29332221

Documents

Biacore Insight Evaluation Software · 2018-11-06 · gelifesciences.com Data file, KA1995180518DF Label-free interaction analysis Biacore™ Insight Evaluation Software Biacore™