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COVID 19/WARN Informational Webinar June 3, 2020 Presented by: VA WARN Program

COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

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Page 1: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

COVID 19/WARNInformational Webinar

June 3, 2020Presented by: VA WARN Program

Page 2: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Welcome• State Agency Partners• VA WARN Members• VA Utilities• Additional VA Partners

Page 3: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Special Guests

• Dr. Marcia Degen, Ph.D, PEEnvironmental Technical Services ManagerVirginia Department of HealthDivision of Water and Wastewater [email protected]

• Dr. Raul Gonzalez, Ph.DEnvironmental ScientistHampton Roads Sanitation District (HRSD)[email protected]

Page 4: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

10

Wastewater Epidemiology

Wastewater epidemiology uses markers in raw wastewater to gather data on the population within a wastewater catchment.

Molecular Source Tracking

Wastewater Surveillance

Page 5: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

11

Sewersheds – Similar to Watersheds

• Similar to watersheds• Sample further

‘downstream’ to get big picture

• Can isolate ‘branches’ to locate hot spots

Page 6: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

12

Sampling COVID-19

o COVID-19 is shed in feceso Most studies suggest the shed virus is not viableo (the virus is highly susceptible to standard disinfection methods)o Data on rate of shedding over course of disease limited

oKey parameter used to convert viral concentration in sewage to approximate number of infections in area (population projection)

oProjections are being refined as new data become availableo Sampling viral RNA using a PCR method

o No ‘standard’ method, but working toward best practiceso Data on the effect of the wastewater matrix on the virus is limited.o Data on any transformations of the virus in-sewer transport limited.

Page 7: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

13

Gertjan Medema, KWRWater Research Institute, 2020, Virtual International Research Summit

Page 8: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

14

Data in grey incomplete

Why Conduct WW Surveillance?Human Testing WW Testing

Individual testing is limited Testing at WWTP captures a large population

It takes 2-14 days for a person to become symptomatic Infected persons begin shedding virus in feces within 2-3 days

Page 9: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

15

Knowledge Gaps

• Rate of fecal shedding – being refined• Consistent methods for sample collection, storage and analysis• Unique challenges working in wastewater – low recovery rates,

interferences• Consistent method to translate viral load to infected population counts

CDC is conducting a Phase One project to answer most of these questions. Water Research Foundation & EPA – seeking labs to participate in study of methods assessment.

Page 10: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

16

Summary

Tracking disease prevalence through wastewater surveillance is a relatively new science and COVID-19 is a new virus.

Surveilling wastewater for COVID 19 may provide some additional information to inform re-opening decisions or signal a resurgence of the disease.

In order for the wastewater surveillance to be useful, it has to be widespread, frequent and long term.

VDH is monitoring the CDC progress in developing consistent guidelines.

Page 11: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

COVID‐19 Surveillance using Wastewater‐Based Epidemiology

Page 12: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

18

HRSD’s Molecular Pathogen Program

• Core Focus Area– Microbial Source Tracking– Quantitative Microbial Risk

Assessment– Pathogen Quantification

• Matrices– Stormwater, Biosolids, Wastewater,

Water Reuse, Shellfish, Sediment

• Capabilities– Digital PCR– Quantitative PCR– Next generation sequencing

Illumina iSeq 100 Nanopore MinION

– Culture: Traditional FIB Coliphage GB-124

Page 13: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Dec 8 First patient

develops symptoms

Jan 11 1st known

death due to new

coronavirus

Jan 30Public Health Emergency of International

Concern

Feb 29 US reports

first COVID-19 death

March 91st HRSD sample

March 11COVID-19 declared pandemic

COVID‐19 Timeline

19

Page 14: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Goal: Regional study that fully captures the rise and fall of COVID‐19 cases

Objectives:1. describe the trends in SARS‐CoV‐2 occurrence 2. assess the ‘true’ prevalence of COVID‐19

HRSD COVID‐19 Surveillance

20

Page 15: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

21

HRSD Monitoring

21

Source: https://commons.wikimedia.org/wiki/File:3D_medical_animation_corona_virus.jpg

• Weekly monitoring of 9 major facilities– Final effluent samples– SWIFT Final samples

• Quantification of CDC’s molecular assays

• VDH engagement from the beginning

Page 16: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

SARS‐CoV‐2 Detection

22

Page 17: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Population Normalized Load 

23

Page 18: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Ability to Observe Differing Trends

24

Page 19: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

A Spatial Look at SARS‐CoV‐2

25

Page 20: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

A Closer Look

Page 21: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Regional Loads

27

Page 22: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Regional Percent Carriers

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Page 23: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

29

Questions

Would you be interested in a broader discussion of wastewater surveillance with VDH?

If CDC completes guidance on sample collection and analysis, would you consider following that guidance and sharing your sample results with VDH?

Page 24: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Questions?

30

Dr. Marcia Degen, Ph.D, PEEnvironmental Technical Services ManagerVirginia Department of HealthDivision of Water and Wastewater [email protected]

Dr. Raul Gonzalez, Ph.DEnvironmental ScientistHampton Roads Sanitation District (HRSD)[email protected]

Page 25: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Situation Report/Updates• Current status of pandemic

Page 26: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

VDH COVID‐19 Map (as of June 3rd)

Page 27: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

VDH COVID‐19 Map (as of June 3rd)

Page 28: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

COVID‐19 Surveillance Dashboard ‐ UVA Model

Page 29: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Institute for Health Metrics and Evaluation (IHME)

Page 30: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

COVIDActNow.org

Page 31: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

WARN Updates• Still no Emergency requests to date• New WARN Members

– New River Regional Water Authority– Urbanna Waterworks

• Supply list information– Personal/Household items– Meat/Food shortages

Page 32: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Phase 1 Updates• Face coverings mandated in inside public buildings

• How are Utilities approaching this new mandate?– Will you provide masks for visitors? – How are you informing/enforcing your policies?

Page 33: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Phase 1 Updates• Specific Guidance for Phase 1 for various 

industries can be found here:  virginia.gov/coronavirus/forwardvirginia.

• Preparation for Phase 2– Surveillance of other industries is helpful for advanced planning

– https://www.governor.virginia.gov/media/governorvirginiagov/governor‐of‐virginia/pdf/Virginia‐Forward‐Phase‐Two‐Guidelines.pdf

Page 34: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Best Practices• Use of technology to ease employer burden

• Determine when contractors/visitors should be allowed back in your facilities– Use of billing system to track who might have been in the facility

– Use of computer surveillance to monitor visitors in and around the building

Page 35: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Continuing Education Credit• Water and Wastewater Operator CPE, along 

with Engineering CE credits will be provided for today’s webinar. 

• To receive a transcript for today’s webinar, please answer the final poll question. 

• Transcripts certificates were emailed for all past webinars.  The email would have come from [email protected]‐us.com

• If you didn’t receive your transcripts, please email [email protected]

Page 36: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Questions?• Contact one of the following WARN leaders

– Ike Eisenhart, Iron Hart Consultingike.Eisenhart@ironhart‐consulting.com

– Mike Lynn, Albemarle County Service [email protected]

– Scott Shirley, Western VA Water [email protected]

Page 37: COVID 19/WARN Informational Webinar COVID 19...Mar 20, 2006  · 12 Sampling COVID-19 o COVID-19 is shed in feces ... – Stormwater, Biosolids, Wastewater, Water Reuse, Shellfish,

Thank you for attending and keep the waters flowing!

• Please check the VA WARN website – For a link to today’s presentation– For updated resources and information – To learn how to join VA WARN