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Cord Blood“ The Hidden Treasure of Life ”
Presented By
Asma I. Al-Asad
Laboratory Manager
Damascus, 2009
Dubai Cord Blood & Research Centre (DCRC)
Established in 2006 as a turnkey project
Dubai Cord Blood & Research Centre (DCRC)
Total Cost 17.5 million AED• 5.5 m Equipment.
• 12 m construction.
Total Built area 1303 m2• Ground Floor area 777 m2
• First Floor area 526 m2
Mission
To provide high quality HLA-typed units for transplantation to patients in need.
Goals of DCRC
To build a Cord-Blood Bank that captures the rich ethnic diversity of the Arab population & the Middle East.
To develop a programme which results in the highest quality units exceeding National and International standards.
Engage with the International Registries to include DCRC units.
DCRC Services
Collect, Process, Test and Store Umbilical Cord Blood stem cell to be used for future therapy.
Perform the Molecular HLA Typing test for patients in need.
Perform a search for possible match for the patients worldwide.
Organization Structure
DCRC
Administrative Service Unit
Laboratory Unit
Stem Cell Lab HLA LabFlocytometry
LabCord Blood
Registry Collection
Subunit
Donor Counseling
office
Total No. of stuff (18)
Medical Director : 1 Lab Manager : 1 Registration : 2 Collection & Counseling : 3 Stem Cell Lab : 4 Specialized Lab : 3 Lab Attendance : 2 Cleaner : 2
DCRC international Recognition & agreements
Nov 2006 : Member of International Cord Blood Society (ICBS).
Jan 2007: signed a contract with KSA (Dallah Group) to collect CBU from GCC countries.
June 2008: Registered in Netcord Virtual Office. January 2009: Associate member of FACT-NetCord
Foundation. March 2009: Opened a collection center that is Orient
Stem Cell Center to Collect CBU.
Mixed Cord Blood Bank
Collection Method“In-Utero ”
the placenta is still in Uterus
•Directed Cord Blood Donation= 1365 CBU
•Volunteer Cord Blood Donation= 199 CBU
Statistics(2006-2008)
343
709512
0
100
200
300
400
500
600
700
800
Year 2006 Year 2007 Year 2008
Cord Blood Collections 2006-2008
Year 2006
Year 2007
Year 2008
217126
509
200
424
880
100
200
300
400
500
600
Year 2006 Year 2007 Year 2008
Accepted & Rejected CBUs 2006-2008
Accepted
Rejected
Related & Volunteer Percentage
358
454
141665578
0
100
200
300
400
500
Year 2006 Year 2007 Year 2008
Related
Volunteer
Total CBU stored 1564UAE
BahrainOman
EygptLebanon
CyprusItaly
0
200
400
600
800
1000
1200
1400
1600
Series1 1500 35 15 4 3 2 1
UAE Bahrain Oman Eygpt Lebanon Cyprus Italy
3 Units → Transplants
_ University of Minnesota Cancer Centre/USA.
_ King Hussein Cancer Centre/ Jordan.
_ Royal Marsden Hospital/ UK.
47 Units → Allocated for patients.
Used & Allocated Units
Cord-Blood Processing2006- 2009: Manually
April 2009: Automated By Sepax System
Method: Volume reduction by depletion. Clean room Temp=14-19˚Celcius Pressure = 25 Pascals Humidity = 45%
Processing Criteria Volume of accepted cord blood ≥ 40 ml. Nucleated cell count ≥ 6.0 x 106 cells ml-1. Viability of cells ≥ 90%. Pre-CD34 ≥ 106 cells ml-1. Recovery rate ≥ 60%.
CryopreservationThe Final Volume stored= 25ml
The CBU is stored in monitored liquid Nitrogen tanks in -196˚C, for ~ 15-20 years.
Extremely low temperatures inhibit the cellular activities when a cryo-preservative is used.
Cord blood has been successfully used to treat over 70 different diseases to date.
Don’t Throw it Away…
Cord Blood“ The Hidden Treasure of Life ”
Presented By
Asma I. Al-Asad
Laboratory Manager
Damascus, 2009
